CN115736145A - Anti-fatigue functional beverage and preparation method thereof - Google Patents
Anti-fatigue functional beverage and preparation method thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to an anti-fatigue functional beverage and a preparation method thereof, wherein the beverage comprises the following formula in parts by weight: 10-30 parts of polygonatum polysaccharide, 0.1-1 part of ginseng polysaccharide, 20-60 parts of erythritol, 0.1-0.5 part of taurine, 0.05-0.15 part of caffeine, 0.01-0.05 part of mint ice and 0.1 x 10 parts of vitamin B ‑3 ‑0.8×10 ‑3 Part(s) of vitamin B12.1 × 10 ‑6 ‑1.0×10 ‑6 200-1000 parts of water.
Description
Technical Field
The invention belongs to the field of functional beverages, and particularly relates to an anti-fatigue functional beverage and a preparation method thereof.
Background
Fatigue is mainly classified into exercise-induced fatigue and chronic fatigue. Wherein, exercise fatigue is a phenomenon of temporary decline of body functions caused by exercise, and can be relieved by supplementing amino acids, traditional Chinese medicines or vitamin functional foods. Delaying the generation of fatigue and promoting the elimination of fatigue, namely resisting fatigue, is a hot problem in the research of the sports medicine and the health care medicine at present. Health care products with the effects of resisting fatigue, enhancing immunity, resisting aging, helping sleep and the like are increasingly concerned by consumers, and particularly, functional beverages with the function of resisting fatigue are favored.
The rhizoma Polygonati contains main chemical components such as polysaccharide, steroid saponin, anthraquinone, alkaloid, etc., and has effects of reducing blood sugar, reducing blood lipid, resisting aging, resisting inflammation, resisting tumor, resisting osteoporosis, resisting atherosclerosis, and resisting fatigue. Ginseng is a traditional Chinese medicinal material in China, commonly known as the king of hundred grass. The ginseng polysaccharide is one of effective components in ginseng, and has biological activities of immunoregulation, tumor resistance, radiation resistance, blood sugar reduction, hematopoietic function regulation and the like.
With the acceleration of the pace of life of modern people, social activities such as work, study and the like cause people to be increasingly tired. Therefore, the research and development of the anti-fatigue functional beverage have quite important significance. The applicant previously developed a polygonatum enzyme beverage (CN 202011240679.3) which shows a certain anti-fatigue effect; the invention further uses the polygonatum polysaccharide and the ginseng polysaccharide as functional components, and provides a functional beverage with more remarkable anti-fatigue function and a preparation method thereof.
Disclosure of Invention
The invention provides an anti-fatigue functional beverage, which is characterized by comprising the following components in parts by weight: 10-30 parts of polygonatum polysaccharide, 0.1-1 part of ginseng polysaccharide, 20-60 parts of erythritol, 0.1-0.5 part of taurine, 0.05-0.15 part of caffeine, 0.01-0.05 part of mint ice and 0.1 x 10 parts of vitamin B -3 -0.8×10 -3 The weight portion of vitamin B12.1 is multiplied by 10 -6 -1.0×10 -6 200-1000 parts of water.
Another embodiment of the present invention provides a method for preparing the above anti-fatigue functional beverage, characterized by comprising the steps of:
(1) Weighing rhizoma Polygonati polysaccharide, ginseng radix polysaccharide, erythritol, taurine, caffeine, mentholum, vitamin B6, vitamin B12, and water according to formula ratio, and homogenizing in a mixing tank;
(2) Pumping the homogenized mixed solution into a filling machine, performing hot filling at 90 ℃, and sealing after filling to obtain a finished product;
(3) And (3) putting the finished product obtained in the step (2) into a sterilization kettle, heating to 130-140 ℃, sterilizing for 10-20min, cooling to 45 ℃, taking out the finished product, spraying codes on the finished product, and packaging to obtain the anti-fatigue functional beverage.
The homogenizing in the step (1) is specifically operated as follows: setting the preheating temperature to 65 ℃, homogenizing twice, wherein the first homogenizing pressure is 30MPa, and the second homogenizing pressure is 20MPa, and standing the homogenized mixture for 2h without layering and precipitation.
Another embodiment of the present invention provides the use of the above anti-fatigue functional beverage as a food or beverage for relieving fatigue and supplementing energy.
Compared with the prior art, the invention has the advantages that: the sealwort-ginseng polysaccharide beverage can obviously improve the suffocation and movement abilities of zebra fish and has the function of delaying fatigue; when the concentration exceeds 400 mug/mL, the asphyxia time can be prolonged; in the suffocation behavior experiment, 400-800 mu g/mL can obviously improve the swimming distance and speed of the embryo. Compared with the previously developed polygonatum enzyme beverage (CN 202011240679.3), the method has the advantages of obviously prolonging the asphyxia time and the like.
Drawings
Figure 1 is a graph of the effect of duration of asphyxia under hypoxic conditions in various groups of zebrafish (. <0.05 compared to the blank group);
figure 2 is a graph of the motor effect of groups of zebrafish embryos after hypoxic stimulation (p <0.05 compared to the blank group).
Detailed Description
Example 1
The formula is as follows: 10 parts of polygonatum polysaccharide, 0.1 part of ginseng polysaccharide, 20 parts of erythritol, 0.1 part of taurine, 0.05 part of caffeine, 0.01 part of mint ice and 0.1 multiplied by 10 parts of vitamin B6 -3 The weight portion of vitamin B12.1 is multiplied by 10 -6 200 parts of water.
Example 2
The formula is as follows: 20 parts of polygonatum polysaccharide, 0.5 part of ginseng polysaccharide, 40 parts of erythritol, 0.3 part of taurine, 0.1 part of caffeine, 0.03 part of mint ice and 0.5 multiplied by 10 parts of vitamin B6 -3 The weight portion of vitamin B12.5 is multiplied by 10 -6 600 parts of water.
Example 3
The formula is as follows: 30 parts of polygonatum polysaccharide, 1 part of ginseng polysaccharide, 60 parts of erythritol, 0.5 part of taurine, 0.15 part of caffeine, 0.05 part of menthol and 0.8 multiplied by 10 parts of vitamin B6 -3 The weight portion of vitamin B12.0 is multiplied by 10 -6 1000 parts of water.
An anti-fatigue functional beverage was prepared according to the formulation of examples 1-3 above by the following method:
the method comprises the following steps: weighing polygonatum polysaccharide, ginseng polysaccharide, erythritol, taurine, caffeine, menthol, vitamin B6, vitamin B12 and water according to the formula ratio, placing the mixture in a mixing tank for homogenization, setting the preheating temperature to be 65 ℃, and homogenizing for two times, wherein the first homogenization pressure is 30MPa, and the second homogenization pressure is 20MPa, and after homogenization, placing the mixture for 2 hours without layering and precipitation;
step two: pumping the homogenized mixed solution into a filling machine, performing hot filling at 90 ℃, and sealing after filling to obtain a finished product;
step three: and (3) putting the filled and sealed finished product into a sterilization kettle, heating to 130-140 ℃, sterilizing for 10-20min, cooling to 45 ℃, taking out the finished product, spraying codes on the finished product, and packaging to obtain the anti-fatigue functional beverage (marked as the finished product in the embodiment 1, the finished product in the embodiment 2 and the finished product in the embodiment 3 respectively).
Example 4 evaluation of anti-fatigue Effect of the beverage of the present invention Using Zebra Fish model
9hpf (horse post fertilization) AB type zebra fish is used as an experimental animal model to study the influence of the components of the polysaccharide drink with different concentrations on the hypoxia tolerance and swimming capability of the zebra fish. The drug and the embryo are incubated for 72h, the asphyxia time of the embryo and the influence on the swimming ability are observed under the extreme hypoxia condition, and statistical analysis is carried out.
1. Materials and methods
(1) Main reagent and its preparation
The polysaccharide beverage is prepared into 80mg/mL mother solution by embryo culture water and placed at the temperature of minus 4 ℃ for standby; salidroside is purchased from Sigma, and prepared into 1mg/mL mother liquor for later use.
(2) Laboratory animal
The experimental zebra fish is AB-series zebra fish, and the fish species are selected from zebra fish drug screening center of Shandong academy of sciences. The culture and propagation of zebra fish are referred to the Westerfield method. The day before egg taking, the male and female fish are separated, the male and female fish (1. Cleaning the fertilized eggs with clear water, and then putting the fertilized eggs into an incubator at 28 ℃ for culture.
(3) Dosing treatment
Eggs that developed for 24h were pipetted onto 12-well plates, and 2000. Mu.L of embryo culture was added to each well, 10 eggs per group. The blank group was not treated, the rhodiola rosea group was treated at 100. Mu.g/mL for 72 hours, and the test group (finished product of example 1) was treated at final concentrations of 100, 200, 400, 800. Mu.g/mL per well for 72 hours.
(4) Study of hypoxic asphyxia
According to comparison, the asphyxiation time (accurate to minute) of the experimental fish which can endure anoxia is accurately measured by taking a mammal model technology as a reference and adopting a 25mL small-mouth glass bottle as an asphyxia chamber in an incubator at 25 ℃ and adding culture water containing 0.03mg/L of oxygen.
(5) Study of behaviours
After the zebra fish asphyxia experiment is finished, a 48-pore plate is used as a ethological trajectory for analysis, 8 embryos in each experimental group are placed in a 24-pore plate manned incubator and incubated for 1h, then the 24-pore plate is placed in a dark box of a zebra fish ethological analysis system, zeblab software is used for collecting the total wandering distance, the wandering time and the circling value of each group within 20min respectively, wherein the wandering speed is obtained from the total distance and the wandering speed, and the values are counted respectively.
(6) Statistical method
Statistical analysis is carried out on experimental data by adopting data statistical software SPSS, the difference between groups is judged by adopting one-factor variance analysis, and the tested difference is considered to have statistical significance if P is less than or equal to 0.05.
2. Results of the experiment
(1) EXAMPLE 1 Effect of the product on the duration of asphyxia in zebra fish in anoxic conditions (FIG. 1)
And (3) conventional observation: all groups of zebra fish have no death and deformity in the process of drug incubation for 3 days, normal groups of zebra fish have no abnormal growth, and compared with blank groups, the drug groups have the characteristics of reduced yolk and increased body length. 400-800 g/L of the polysaccharide beverage component has the capability of obviously prolonging the exercise time under the anoxic condition (p is less than 0.05) compared with a blank group.
(2) EXAMPLE 1 Effect of the product on the locomotion of zebra fish after hypoxic stimulation (FIG. 2)
Counting the behavior tracks of each group of fish bodies within 20 minutes, wherein the statistical result shows that the rhodiola rosea group has significant difference (p is less than 0.05) compared with a blank group, and the motion distance and the motion average speed (p is less than 0.05); compared with a control group, 400-800 mug/mL of the polysaccharide beverage component has significant difference in movement distance (p < 0.05) and movement speed (p < 0.05), and shows dose dependence.
Example 5
The finished product of example 2, example 3 and example 3 of the prior patent application (CN 202011240679.3) of the present invention were tested according to the method of example 4, wherein the formulation is 50 parts of a Taishan sealwort ferment stock solution, 60 parts of erythritol, 0.5 part of taurine, 0.15 part of caffeine, 0.05 part of menthol, 0.8X 10 parts of vitamin B6 -3 The weight portion of vitamin B12.0 is multiplied by 10 -6 1000 parts of water. ) The prepared polygonatum sibiricum enzyme anti-fatigue beverage (hereinafter referred to as a patent product) has the effects on the suffocation duration of zebra fish under the anoxic condition and the movement of the zebra fish after anoxic stimulation; the results show that the finished products of example 2 and example 3 of the invention have the ability to significantly prolong exercise time under anoxic conditions (p) in the concentration range of 200-800 mug/mL compared with the blank group<0.05 ); especially when the concentration is 400-800 mu g/mL, the oxygen-deficient condition is obviously prolongedThe dynamic time capability is slightly better than that of the finished product of the example 1 (the extension is about 210-268 min). On the contrary, the patent product has the effect on the asphyxia duration of zebra fish under the anoxic condition at the concentration of 100, 200 and 400 mu g/mL, is basically consistent with the blank group, has no obvious difference, and has certain prolongation of the movement time under the anoxic condition (the prolongation is 58 min) compared with the blank group only when the concentration reaches 800 mu g/mL.
The experimental conclusion is that: the polysaccharide beverage (anti-fatigue beverage) can obviously improve the suffocation and movement abilities of zebra fish and has the function of delaying fatigue.
Claims (7)
1. The anti-fatigue functional beverage is characterized by comprising the following components in parts by weight: 10-30 parts of polygonatum polysaccharide, 0.1-1 part of ginseng polysaccharide, 20-60 parts of erythritol, 0.1-0.5 part of taurine, 0.05-0.15 part of caffeine, 0.01-0.05 part of menthol, and 0.1X 10 parts of vitamin B6 -3 -0.8×10 -3 The weight portion of vitamin B12.1 is multiplied by 10 -6 -1.0×10 -6 200-1000 parts of water.
2. The fatigue-resistant functional beverage according to claim 1, wherein the beverage has the following formula in parts by weight: 10 parts of polygonatum polysaccharide, 0.1 part of ginseng polysaccharide, 20 parts of erythritol, 0.1 part of taurine, 0.05 part of caffeine, 0.01 part of mint ice and 0.1 multiplied by 10 parts of vitamin B6 -3 The weight portion of vitamin B12.1 is multiplied by 10 -6 200 parts of water.
3. The fatigue-resistant functional beverage according to claim 1, wherein the beverage has the following formula in parts by weight: 20 parts of polygonatum polysaccharide, 0.5 part of ginseng polysaccharide, 40 parts of erythritol, 0.3 part of taurine, 0.1 part of caffeine, 0.03 part of mint ice and 0.5 multiplied by 10 parts of vitamin B6 -3 The weight portion of vitamin B12.5 is multiplied by 10 -6 Parts by weight, and 600 parts by weight of water.
4. The fatigue-resistant functional beverage according to claim 1, wherein the beverage is formulated as follows in parts by weight: 30 parts of polygonatum polysaccharide, 1 part of ginseng polysaccharide, 60 parts of erythritol, 0.5 part of taurine, 0.15 part of caffeine and 0 part of menthol.05 parts of vitamin B6, 0.8 multiplied by 10 -3 The weight portion of vitamin B12.0 is multiplied by 10 -6 1000 parts of water.
5. The method for preparing an antifatigue functional beverage according to any one of claims 1-4, characterized by comprising the steps of:
(1) Weighing rhizoma Polygonati polysaccharide, ginseng radix polysaccharide, erythritol, taurine, caffeine, mentholum, vitamin B6, vitamin B12, and water according to formula ratio, and homogenizing in a mixing tank;
(2) Pumping the homogenized mixed solution into a filling machine, performing hot filling at 90 ℃, and sealing after filling to obtain a finished product;
(3) And (3) putting the finished product obtained in the step (2) into a sterilization kettle, heating to 130-140 ℃, sterilizing for 10-20min, cooling to 45 ℃, taking out the finished product, spraying codes on the finished product, and packaging to obtain the anti-fatigue functional beverage.
6. The method of claim 5, wherein the homogenizing in step (1) is carried out by: setting the preheating temperature to 65 ℃, homogenizing twice, wherein the first homogenizing pressure is 30MPa, and the second homogenizing pressure is 20MPa, and standing the homogenized mixture for 2h without layering and precipitation.
7. Use of the anti-fatigue functional beverage according to any one of claims 1 to 4 as a food or drink for alleviating fatigue and supplementing energy.
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| CN112426479A (en) * | 2020-11-17 | 2021-03-02 | 成都三勒浆药业集团四川华美制药有限公司 | Composition with effects of resisting fatigue and enhancing immunity and application thereof |
| CN112438353A (en) * | 2020-11-09 | 2021-03-05 | 山东省科学院生物研究所 | Rhizoma polygonati enzyme anti-fatigue beverage and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112438353A (en) * | 2020-11-09 | 2021-03-05 | 山东省科学院生物研究所 | Rhizoma polygonati enzyme anti-fatigue beverage and preparation method thereof |
| CN112426479A (en) * | 2020-11-17 | 2021-03-02 | 成都三勒浆药业集团四川华美制药有限公司 | Composition with effects of resisting fatigue and enhancing immunity and application thereof |
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