CN115735822A - Method for breeding parent Litopenaeus vannamei of Guangtai No. 1 with high reproductive capacity - Google Patents

Method for breeding parent Litopenaeus vannamei of Guangtai No. 1 with high reproductive capacity Download PDF

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CN115735822A
CN115735822A CN202211519650.8A CN202211519650A CN115735822A CN 115735822 A CN115735822 A CN 115735822A CN 202211519650 A CN202211519650 A CN 202211519650A CN 115735822 A CN115735822 A CN 115735822A
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parent
shrimp
weight
female
feeding
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黄皓
蔡重志
何强
黄昊鹏
王芬彬
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Bohai Aquatic Breeding Hainan Co ltd
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Abstract

The invention provides a method for breeding parent litopenaeus vannamei 'Guangtai No. 1' with high reproductive capacity. The method comprises the following steps: (1) Selecting a culture pond, wherein natural light is adopted for illumination, the salinity of water is 28-30 per mill, the water temperature is 28-30 ℃, and the pH value is 8-8.5; selecting parent shrimp and female shrimp with the specification of not less than 55 g/tail, male shrimp with the specification of not less than 35 g/tail, and parent shrimp cultivation density of 10-20 tails/m 2 And (2) during the parent shrimp cultivation period, the feed is clamworm, oyster and squid, and the specific feeding proportion and feeding amount are as follows: in the stage I-II of the ovaries of female shrimps, the weight ratio of the clamworm to the oyster to the squid is 50-60: 20 to 30:15 to 25; in the female shrimp ovary stage III-IV, the feeding weight ratio of the clamworm, the oyster and the squid is 30-40: 35 to 45 parts of: 20 to 30 percent; in the V-VI stage of female shrimp ovary, the feeding weight ratio of clamworm, oyster and squid is 5-15: 65 to 75:15 to 25; the compound bacterial powder is added and fed to female shrimps in the ovary V-VI stage. By adopting the breeding method, the reproductive capacity of parent shrimps is effectively improved, the egg laying amount and egg laying quality are improved, and the hatching rate is improved。

Description

Method for breeding parent litopenaeus vannamei 'Guangtai No. 1' with high reproductive capacity
Technical Field
The invention relates to the field of prawn culture, in particular to a method for culturing parent shrimps of Litopenaeus vannamei 'Guangtai No. 1' with high reproductive capacity.
Background
The new variety of the penaeus vannamei boone, namely Guangtai No. 1, is a variety which is jointly developed by the patent applicant, a marine institute of Chinese academy of sciences and northwest agriculture and forestry science and technology university, and is certified by a new variety of Ministry of agriculture in 2017 in 4 months, wherein the variety has a registration number: GS-01-003-2016. The Litopenaeus vannamei 'Guangtai No. 1' adopts the traditional parent shrimp cultivation method of the Litopenaeus vannamei variety, has small egg laying amount and hatching rate, and is not beneficial to the enlargement of seed production scale. Therefore, a parent shrimp cultivation method is urgently needed, aiming at the 'Guangtai No. 1' of the litopenaeus vannamei, the problems are overcome.
Disclosure of Invention
In view of the above, the invention provides a parent shrimp cultivation method of the litopenaeus vannamei 'guangtai No. 1' with high reproductive capacity, which effectively improves the reproductive capacity of the parent shrimps, improves the egg laying amount and the egg laying quality, and improves the hatching rate.
The technical scheme of the invention is realized as follows:
a method for breeding parent Litopenaeus vannamei of Guangtai No. 1 with high reproductive capacity comprises the following steps:
(1) Selecting a culture pond, wherein natural light is adopted for illumination, the salinity of water is 28-30 per mill, the water temperature is 28-30 ℃, and the pH value is 8-8.5; selecting parent shrimp female shrimp with specification not less than 55 g/tail and male shrimp with specification not less than 35 g/tail, and performing virus detection without WSV, IHHNV and TSV, wherein the parent shrimp culture density is 10-20 tail/m 2
(2) During parent shrimp cultivation, the feed is clamworm, oyster and squid, the feed feeding proportion and the feeding amount are controlled according to different development periods of gonads of female shrimps, and the specific feeding proportion and the feeding amount are as follows: in the ovary I-II stage of female shrimps, the weight ratio of the clamworm, the oyster and the squid fed by the feed is 50-60: 20 to 30:15 to 25 percent, wherein the daily feeding amount is 1 to 2 percent of the weight of the parent shrimps; in the female shrimp ovary stage III-IV, the feeding weight ratio of the clamworm, the oyster and the squid is 30-40: 35 to 45 parts by weight: 20-30 percent, and the daily feeding amount is 2-3 percent of the weight of the parent shrimps; in the V-VI stage of female shrimp ovary, the feeding weight ratio of clamworm, oyster and squid is 5-15: 65-75: 15 to 25 percent, the daily feeding amount is 4 to 5 percent of the weight of the parent shrimps; feeding composite bacteria powder in an increased amount of 0.8-1.2% of the weight of parent shrimps in the V-VI stage of ovaries of female shrimps, wherein the composite bacteria powder is prepared from black glutinous rice flour, seaweed powder, jackfruit seed powder and lactobacillus plantarum HNU082 bacteria powder. The fungus powder is fed in the V-VI stage of the ovaries of female shrimps, the black glutinous rice flour and the jackfruit seed powder are matched to better improve the reproductive capacity of the female shrimps, and the egg laying quality of the female shrimps is improved together with the seaweed powder and the lactobacillus plantarum HNU082 fungus powder.
The female shrimp ovary I-VI stage is female shrimp ovary forming stage, breeding stage, small growth stage, large growth stage, mature stage and recovery stage.
Further, the composite bacterial powder is prepared from 50-60 parts by weight of black glutinous rice flour, 10-20 parts by weight of seaweed meal, 15-25 parts by weight of jackfruit seed meal and 8-12 parts by weight of lactobacillus plantarum HNU082 bacterial powder. Preferably, the composite bacterial powder is prepared from 55 parts by weight of black glutinous rice powder, 15 parts by weight of seaweed meal, 20 parts by weight of jackfruit seed powder and 10 parts by weight of lactobacillus plantarum HNU082 bacterial powder.
Preferably, in the ovary I-II stage of female shrimps, the feeding weight ratio of the clamworms, the oysters and the squids is 55:25:20; in the stage III-IV of female shrimp ovary, the feeding weight ratio of clamworm, oyster and squid is 35:40:25; in the V-VI stage of female shrimp ovary, the feeding weight proportion of clamworm, oyster and squid is 10:70:20.
further, the parent shrimp breeding density is 15 tails/m 2
Furthermore, each pond water body of the parent shrimp breeding pond is larger than 24-35m 3 (ii) a 28-35 120 meshes of air stones are arranged in each pool.
Furthermore, water is changed once a day, and the water change amount is 2/3.
Further, the feed is fed for 2-4 times a day.
Compared with the prior art, the invention has the beneficial effects that:
(1) Aiming at the Guangtai No. 1 of the penaeus vannamei, the parent shrimp breeding method is adopted, the egg laying period of the parent shrimp is controlled to be 7-8 days, the egg laying amount is over 32 ten thousand per tail, the hatching rate is over 93 percent, and the seed production scale of the Guangtai No. 1 of the penaeus vannamei is favorably expanded.
(2) The parent shrimp cultivation method aims at the situation that the south America white shrimps are Guangtai No. 1, during the parent shrimp cultivation period, the feed is clamworm, oyster and squid, the feeding proportion and the feeding amount of the clamworm, the oyster and the squid are controlled according to different development periods of gonads of female shrimps, and the feeding of the female shrimps is increased in the V-VI period, and the compound bacterium powder prepared from black glutinous rice flour, seaweed powder, jackfruit seed powder and lactobacillus plantarum HNU082 bacterium powder is fed, so that the parent shrimp reproductive capacity is further improved, the disease resistance of the parent shrimps is improved, the egg laying amount and the egg laying quality are improved, and the hatching rate is improved.
(3) According to the invention, the compound bacteria powder is fed in the female shrimp ovary V-VI stage, the black glutinous rice powder and the jackfruit seed powder are matched to better improve the reproductive capacity of the 'Guangtai No. 1' parent shrimps of the penaeus vannamei, and the compound bacteria powder, the seaweed powder and the lactobacillus plantarum HNU082 bacteria powder jointly improve the egg laying quality of the 'Guangtai No. 1' parent shrimps of the penaeus vannamei.
Drawings
FIG. 1 is a technical route of seed production of Litopenaeus vannamei Guangtai No. 1.
Detailed Description
In order that the technical contents of the invention may be better understood, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
The invention uses lactobacillus plantarum HNU082 bacterial powder, and HNU082 bacterial strain is preserved in Guangdong province microorganism culture collection center (GDMCC), and the preservation number is GDMCC NO:61552, deposit address: the fifth building of the experimental building of microbial institute, 100 province, of the first furious Zhonglu, guangzhou, china is named according to taxonomy: lactobacillus plantarum (Lactplantibibacillus plantarum).
The seed production method of the parent shrimp (commercial generation (ABCD)) of the litopenaeus vannamei 'Guangtai No. 1' is shown in figure 1. The bred fast growing line (line A) is used as a male parent to mate with a female parent of the resistant/high breeding line (line B) to produce a male parent line AB line in a parent generation, the bred fast growing line (line C) is used as a male parent to mate with the high breeding line (line D) to produce a female line CD line in the parent generation, and the male parent (line AB) and the female parent (line CD) of the parent generation mate to produce commercial generation (ABCD) offspring.
Example 1
The method for breeding the parent Litopenaeus vannamei of Guangtai No. 1 comprises the following steps:
(1) Selecting a cultivation pond, soaking the parent shrimp cultivation pond in a 100ppm bleaching powder full pond before use, washing, and then washing with fresh water; all tools are used after being soaked and washed clean by 100ppm bleaching powder; 28-35 air stones of 120 meshes are arranged in each pond, and the water body of each pond of the parent shrimp breeding pond is more than 24-35m 3 Changing water once every day, wherein the water changing amount is 2/3, the water for parent shrimp culture is directly used after secondary filtration, the water source quality conforms to the regulation of GB 11607-89, and the seawater quality conforms to the regulation of NY 5052-2001; the illumination adopts half-top-shading natural light, the salinity of water is 28 per mill-30 per mill, the water temperature is 28-30 ℃, and the pH value is 8-8.5; selecting parent shrimp and female shrimp with the specification of not less than 55 g/tail and male shrimp with the specification of not less than 35 g/tail, and detecting viruses without WSV, IHHNV and TSV, wherein the parent shrimp culture density is 15 tail/m 2
The aeration was performed using a 0.75kW Roots blower, with a 0.2kW wheel blower on standby. The water for cultivating the parent shrimps is a 6kW centrifugal pump, is subjected to rough filtration and uniform extraction at seaside, is filtered by a sand filter and then enters a sedimentation tank for sedimentation, is subjected to disinfection treatment for 24 hours by 100ppm of bleaching powder (the content of available chlorine is not less than 30 percent), is subjected to uninterrupted aeration and sedimentation for 72 hours, and is filtered and injected into each water tank after residual chlorine naturally disappears.
(2) During parent shrimp cultivation, the feed is clamworm, oyster and squid, the feeding is carried out for 3 times every day, the feed feeding proportion and the feeding amount are controlled according to different development periods of gonads of female shrimps, and the specific feeding proportion and the feeding amount are as follows:
in the ovary I-II stage of female shrimps, the feeding weight ratio of clamworms, oysters and squids is 60:20:20, the daily feeding amount is about 1.5 percent of the weight of the parent shrimps; in the stage III-IV of female shrimp ovary, the feeding weight ratio of clamworm, oyster and squid is 40:35:25, feeding the parent shrimps by about 2.5 percent of the weight of the parent shrimps every day; in the V-VI stage of female shrimp ovary, the feeding weight proportion of clamworm, oyster and squid is 15:65:20, feeding the parent shrimps by about 4.5 percent of the weight of the parent shrimps every day;
and (3) adding compound bacteria powder into the female shrimp at the ovary V-VI stage, wherein the feeding amount is about 1 percent of the weight of the parent shrimp, and the compound bacteria powder is prepared by mixing black glutinous rice flour, seaweed powder, jackfruit seed powder and lactobacillus plantarum HNU082 bacteria powder. The feed comprises, by weight, 52 parts of black glutinous rice flour, 15 parts of seaweed meal, 25 parts of jackfruit seed meal and 8 parts of lactobacillus plantarum HNU082 fungus powder.
Example 2
The method for breeding the parent Litopenaeus vannamei of Guangtai No. 1 comprises the following steps:
(1) Selecting a cultivation pond, soaking the parent shrimp cultivation pond in a 100ppm bleaching powder full pond before use, washing, and then washing with fresh water; all tools are used after being soaked and washed clean by 100ppm bleaching powder; 28-35 aerostones of 120 meshes are arranged in each pond, and the water body of each pond of the parent shrimp breeding pond is more than 24-35m 3 Changing water once every day, wherein the water changing amount is 2/3, the water for parent shrimp culture is directly used after secondary filtration, the water source quality conforms to the regulation of GB 11607-89, and the seawater quality conforms to the regulation of NY 5052-2001; the illumination adopts half-top-shading natural light, the salinity of water is 28 per mill-30 per mill, the water temperature is 28-30 ℃, and the pH value is 8-8.5; selecting parent shrimp female shrimp with specification not less than 55 g/tail and male shrimp with specification not less than 35 g/tail, and performing virus detection without WSV, IHHNV and TSV, wherein the parent shrimp culture density is 15 tail/m 2
The inflation uses a 0.75kW Roots blower, and a 0.2kW wheel blower is reserved. The water for cultivating the parent shrimps is a 6kW centrifugal pump, is subjected to rough filtration and uniform extraction at seaside, is filtered by a sand filter and then enters a sedimentation tank for sedimentation, is subjected to disinfection treatment for 24 hours by 100ppm of bleaching powder (the content of available chlorine is not less than 30 percent), is subjected to uninterrupted aeration and sedimentation for 72 hours, and is filtered and injected into each water tank after residual chlorine naturally disappears.
(2) During parent shrimp cultivation, the feed is clamworm, oyster and squid, the feeding is carried out for 3 times every day, the feed feeding proportion and the feeding amount are controlled according to different development periods of gonads of female shrimps, and the specific feeding proportion and the feeding amount are as follows:
in the ovary I-II stage of female shrimps, the weight ratio of feeding clamworms, oysters and squids is 50:30:20, the daily feeding amount is about 1.5 percent of the weight of the parent shrimps; in the stage III-IV of female shrimp ovary, the feeding weight ratio of clamworm, oyster and sleeve-fish is 30:45:25, the daily feeding amount is about 2.5 percent of the weight of the parent shrimps; in the V-VI stage of female shrimp ovary, the feeding weight ratio of clamworm, oyster and squid is 10:75:15, the daily feeding amount is about 4.5 percent of the weight of the parent shrimps;
feeding compound bacteria powder in an increased feeding amount of about 1% of the weight of parent shrimps in the V-VI stage of ovaries of female shrimps, wherein the compound bacteria powder is prepared by mixing black glutinous rice flour, seaweed powder, jackfruit seed powder and lactobacillus plantarum HNU082 bacteria powder. 60 parts of black glutinous rice flour, 13 parts of seaweed meal, 15 parts of jackfruit seed meal and 12 parts of lactobacillus plantarum HNU082 bacterial powder.
Example 3
The method for breeding the parent Litopenaeus vannamei of Guangtai No. 1 comprises the following steps:
(1) Selecting a cultivation pond, soaking the parent shrimp cultivation pond in a 100ppm bleaching powder full pond before use, washing, and then washing with fresh water; all tools are used after being soaked and washed clean by 100ppm bleaching powder; 28-35 aerostones of 120 meshes are arranged in each pond, and the water body of each pond of the parent shrimp breeding pond is more than 24-35m 3 Changing water once every day, wherein the water changing amount is 2/3, the water for parent shrimp culture is directly used after secondary filtration, the water source quality conforms to the regulation of GB 11607-89, and the seawater quality conforms to the regulation of NY 5052-2001; the illumination adopts half-covered natural light, the salinity of water is 28-30 per mill, the water temperature is 28-30 ℃, and the pH value is 8-8.5; selecting parent shrimp and female shrimp with the specification of not less than 55 g/tail and male shrimp with the specification of not less than 35 g/tail, and detecting viruses without WSV, IHHNV and TSV, wherein the parent shrimp culture density is 15 tail/m 2
The inflation uses a 0.75kW Roots blower, and a 0.2kW wheel blower is reserved. The water for cultivating the parent shrimps is a 6kW centrifugal pump, is subjected to rough filtration and uniform extraction at seaside, is filtered by a sand filter and then enters a sedimentation tank for sedimentation, is subjected to disinfection treatment for 24 hours by 100ppm of bleaching powder (the content of available chlorine is not less than 30 percent), is subjected to uninterrupted aeration and sedimentation for 72 hours, and is filtered and injected into each water tank after residual chlorine naturally disappears.
(2) During parent shrimp cultivation, the feed is clamworm, oyster and squid, and is fed for 3 times a day, according to the different development periods of the gonad of female shrimp, the feed feeding proportion and the feeding amount are controlled, and the specific feeding proportion and the feeding amount are as follows:
in the ovary I-II stage of female shrimps, the weight ratio of feeding clamworms, oysters and squids is 55:25:20, feeding the parent shrimps by about 1.5 percent of the weight of the parent shrimps every day; in the female shrimp ovary III-IV stage, the feeding weight proportion of the clamworm, the oyster and the squid is 35:40:25, feeding the parent shrimps by about 2.5 percent of the weight of the parent shrimps every day; in the V-VI stage of female shrimp ovary, the feeding weight ratio of clamworm, oyster and squid is 10:70:20, the daily feeding amount is about 4.5 percent of the weight of the parent shrimps;
feeding compound bacteria powder in an increased feeding amount of about 1% of the weight of parent shrimps in the V-VI stage of ovaries of female shrimps, wherein the compound bacteria powder is prepared by mixing black glutinous rice flour, seaweed powder, jackfruit seed powder and lactobacillus plantarum HNU082 bacteria powder. The composite bacterial powder is prepared from 55 parts by weight of black glutinous rice powder, 15 parts by weight of seaweed meal, 20 parts by weight of jackfruit seed meal and 10 parts by weight of lactobacillus plantarum HNU082 bacterial powder.
Test examples
Parent shrimp breeding was carried out in the breeding base of Wenchang town, hainan province according to the methods of examples 1 to 3, and female shrimps after mating were bred in a 20:00 and 22:00 times of the water bodies are transferred into a spawning pond with the specification of 400cm multiplied by 600cm multiplied by 120cm, the special paint for aquatic products needs to be coated, and the water body of a single pond is 24m 3 (ii) a The number of 120 meshes of air stones is 5 per square, and the hatching pond is disinfected before use.
The next morning after egg laying 01:30 moving back to the parent shrimp breeding pond. Hatching density is not more than 50 ten thousand/m 3 The water temperature is 28.5 ℃, the light is dim, and no medicine is used during the spawning and hatching period. The eggs and larvae were gently agitated throughout the pond every 30 minutes. Collecting the larvae to a seedling emergence barrel when the larvae grow to flea larvae stage I, eliminating the larvae of the middle and lower layers after stopping air supply, taking the larvae with strong phototaxis of the upper layer, performing microscopic examination on the larvae without abnormal setae, counting, and then entering a seedling raising pond. And respectively counting the egg laying amount of parent shrimps, recording the egg laying period and counting the hatching rate.
TABLE 1 parent shrimp cultivation of Litopenaeus vannamei "Guantai No. 1
Spawning cycle Parent shrimp egg laying amount (Wan/tailed) Hatching rate (%)
Example 1 7-8 days 33.72±0.13 93.21±1.45
Example 2 7-8 days 32.58±0.21 95.32±1.28
Example 3 7-8 days 35.14±0.15 96.87±1.15
By adopting the parent shrimp breeding method, the spawning period of the parent shrimps is controlled to be 7-8 days, the spawning amount is more than 32 ten thousand per tail, and the hatching rate is more than 93%.
Comparative study 1
Based on example 3, the feeding weight ratio of female shrimp ovary stage I-VI clamworm, oyster and squid is adjusted, see Table 2, and the total feeding amount in each stage is the same as that in example 3.
TABLE 2 weight proportion of clam worm, oyster and squid
Nereid larva Oyster shell Squid
Stage I-II 30 30 40
Stage III to IV 30 40 30
Stage V-VI 40 30 30
After the cultivation by the method, compared with the example 3, the spawning period is obviously shortened, and the spawning amount is also reduced. Therefore, according to the feeding weight proportion of the female shrimp ovary stage I-VI clamworm, oyster and squid, the parent shrimp cultivation effect is better.
Comparative study 2
The difference from the embodiment 3 is that the ovary V-VI stage of female shrimp is increased to feed the compound bacterial powder with the feeding amount of about 1 percent of the weight of the parent shrimp, and the compound bacterial powder is prepared by mixing seaweed powder and lactobacillus plantarum HNU082 bacterial powder. The composite bacterial powder is prepared from 50 parts by weight of seaweed powder and 50 parts by weight of Lactobacillus plantarum HNU082 bacterial powder. After the cultivation by the method, the egg laying amount is obviously reduced compared with that of the example 3.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims (9)

1. A method for breeding the parent Litopenaeus vannamei Guangtai No. 1 with high reproduction capacity,
(1) Selecting a culture pond, wherein natural light with a half-covered top is adopted for illumination, the salinity of water is 28-30 per mill, the water temperature is 28-30 ℃, and the pH value is 8-8.5; selecting parent shrimp female shrimp with specification not less than 55 g/tail and male shrimp with specification not less than 35 g/tail, and performing virus detection without WSV, IHHNV and TSV, wherein the parent shrimp culture density is 10-20 tail/m 2
(2) During the parent shrimp cultivation period, the feed is clamworm, oyster and squid, and the specific feeding proportion and feeding amount are as follows:
in the ovary I-II stage of female shrimps, the weight ratio of the clamworm, the oyster and the squid fed by the feed is 50-60: 20 to 30:15 to 25 percent, wherein the daily feeding amount is 1 to 2 percent of the weight of the parent shrimps;
in the female shrimp ovary stage III-IV, the feeding weight ratio of the clamworm, the oyster and the squid is 30-40: 35 to 45 parts of: 20-30 percent, and the daily feeding amount is 2-3 percent of the weight of the parent shrimps;
in the V-VI stage of female shrimp ovary, the feeding weight ratio of clamworm, oyster and squid is 5-15: 65-75: 15 to 25 percent, the daily feeding amount is 4 to 5 percent of the weight of the parent shrimps;
feeding compound bacteria powder to female shrimps in the ovary V-VI stage, wherein the compound bacteria powder is prepared from black glutinous rice flour, seaweed powder, jackfruit seed powder and lactobacillus plantarum HNU082 bacteria powder.
2. The method for breeding the parent shrimps of the litopenaeus vannamei 'guangtai No. 1' with high reproductive capacity according to claim 1, wherein the compound bacteria powder is prepared from 50-60 parts by weight of black glutinous rice flour, 10-20 parts by weight of seaweed powder, 15-25 parts by weight of jackfruit seed powder and 8-12 parts by weight of lactobacillus plantarum HNU082 bacteria powder.
3. The method for breeding the parent shrimp of Litopenaeus vannamei "Guangtai No. 1" with high reproductive capacity according to claim 1 or 2, wherein the feeding amount of the composite bacterial powder is 0.8-1.2% of the weight of the parent shrimp.
4. The method for breeding Litopenaeus vannamei "Guangtai No. 1" with high reproductive capacity according to claim 1, wherein the parent shrimp breeding density is 15 tails/m 2
5. The method for breeding Litopenaeus vannamei "Guangtai No. 1" with high reproductive capacity as claimed in claim 1, wherein the water body of each pond of the pond is more than 24-35m 3 (ii) a 28-35 120 meshes of air stones are arranged in each pool.
6. The method for breeding Litopenaeus vannamei "Guangtai No. 1" with high reproductive capacity as claimed in claim 1, wherein the water is changed once a day by 2/3.
7. The method for breeding Litopenaeus vannamei "Guantai No. 1" with high reproductive capacity according to claim 1, wherein the parent Litopenaeus vannamei is fed 2-4 times a day.
8. The method for breeding the parent shrimps of the litopenaeus vannamei 'guangtai 1' with high reproductive capacity according to claim 1, wherein in the stage I-II of ovaries of female shrimps, the feeding weight ratio of clamworm, oyster and squid is 55:25:20; in the stage III-IV of female shrimp ovary, the feeding weight ratio of clamworm, oyster and squid is 35:40:25; in the V-VI stage of the ovaries of female shrimps, the feeding weight proportion of the clamworms, the oysters and the squids is 10:70:20.
9. the method for breeding Litopenaeus vannamei Guangtai No. 1 parent shrimp with high reproductive capacity according to claim 2, wherein the compound bacteria powder is prepared from 55 parts by weight of black glutinous rice powder, 15 parts by weight of seaweed powder, 20 parts by weight of jackfruit seed powder and 10 parts by weight of Lactobacillus plantarum HNU082 bacteria powder.
CN202211519650.8A 2022-11-30 2022-11-30 Method for breeding parent Litopenaeus vannamei of Guangtai No. 1 with high reproductive capacity Pending CN115735822A (en)

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