CN115698296A - 编辑靶标rna的方法 - Google Patents

编辑靶标rna的方法 Download PDF

Info

Publication number
CN115698296A
CN115698296A CN202180037500.0A CN202180037500A CN115698296A CN 115698296 A CN115698296 A CN 115698296A CN 202180037500 A CN202180037500 A CN 202180037500A CN 115698296 A CN115698296 A CN 115698296A
Authority
CN
China
Prior art keywords
leu
glu
lys
gly
val
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180037500.0A
Other languages
English (en)
Chinese (zh)
Inventor
一濑瑞穂
B·古曼
八木祐介
赤岩友美
岛尻恭香
中村崇裕
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japanese Gene Editing Co ltd
Original Assignee
Japanese Gene Editing Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japanese Gene Editing Co ltd filed Critical Japanese Gene Editing Co ltd
Publication of CN115698296A publication Critical patent/CN115698296A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202180037500.0A 2020-03-31 2021-03-31 编辑靶标rna的方法 Pending CN115698296A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020-065065 2020-03-31
JP2020065065 2020-03-31
PCT/JP2021/014096 WO2021201198A1 (ja) 2020-03-31 2021-03-31 標的ｒｎａを編集する方法

Publications (1)

Publication Number Publication Date
CN115698296A true CN115698296A (zh) 2023-02-03

Family

ID=77930108

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180037500.0A Pending CN115698296A (zh) 2020-03-31 2021-03-31 编辑靶标rna的方法

Country Status (11)

Country Link
US (1) US20230125942A1 (https=)
EP (1) EP4130274A4 (https=)
JP (4) JP7668547B2 (https=)
KR (1) KR20220165747A (https=)
CN (1) CN115698296A (https=)
AU (1) AU2021248204A1 (https=)
BR (1) BR112022019571A2 (https=)
CA (1) CA3179365A1 (https=)
IL (1) IL296955A (https=)
TW (1) TWI899197B (https=)
WO (1) WO2021201198A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3216313A1 (en) * 2021-04-30 2022-11-03 Masayuki NAKAMORI Myotonic dystrophy type 1 therapeutic drug
US20250302993A1 (en) * 2021-12-24 2025-10-02 GeCoRT Co., Ltd. Enzyme, complex, recombinant vector, therapeutic agent for genetic disorder, and polynucleotide
WO2024249445A2 (en) * 2023-05-26 2024-12-05 The Trustees Of Columbia University In The City Of New York An rna recognition and editing code of pentatricopeptide repeat proteins
CN117384884B (zh) * 2023-11-30 2024-03-08 辉大(上海)生物科技有限公司 IscB多肽及其用途

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2336332A3 (en) * 2008-04-29 2011-11-09 Monsanto Technology LLC Genes and uses for plant enhancement
WO2013058404A1 (ja) * 2011-10-21 2013-04-25 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用
KR102893770B1 (ko) 2013-04-22 2025-11-28 고쿠리쓰다이가쿠호진 규슈다이가쿠 피피알 단백질을 이용하는 dna 결합성 단백질 및 그의 이용

Also Published As

Publication number Publication date
JP7668547B2 (ja) 2025-04-25
US20230125942A1 (en) 2023-04-27
JP2025120313A (ja) 2025-08-15
CA3179365A1 (en) 2021-10-07
WO2021201198A1 (ja) 2021-10-07
JP7698351B2 (ja) 2025-06-25
BR112022019571A2 (pt) 2022-12-06
EP4130274A4 (en) 2024-05-22
IL296955A (en) 2022-12-01
EP4130274A1 (en) 2023-02-08
JP7698371B1 (ja) 2025-06-25
JP2025107239A (ja) 2025-07-17
TW202204382A (zh) 2022-02-01
JP2024096897A (ja) 2024-07-17
KR20220165747A (ko) 2022-12-15
JPWO2021201198A1 (https=) 2021-10-07
TWI899197B (zh) 2025-10-01
AU2021248204A1 (en) 2022-11-10

Similar Documents

Publication Publication Date Title
CN115698296A (zh) 编辑靶标rna的方法
JP7246100B2 (ja) 新規融合タンパク質の調製およびそのタンパク質合成の向上における使用
EP4021945A2 (en) Combinatorial adenine and cytosine dna base editors
CN112585264B (zh) 重组型kod聚合酶
CN109152808A (zh) 用于核酸序列的特异性靶向的二聚蛋白质
Kim et al. Structure of the Hir histone chaperone complex
RU2858663C2 (ru) Способ редактирования рнк-мишени
HK40080660A (zh) 编辑靶标rna的方法
US20250223576A1 (en) Optimized cas protein and use thereof
WO2012033653A1 (en) A composition, method and kit for obtaining purified recombinant proteins
HK40086250A (en) Method for editing target rna
KR102898966B1 (ko) 응집이 적은 피피알 단백질 및 그의 이용
CN114456244A (zh) 基因OsR498G1018986900.01及其编码的蛋白在调控水稻垩白中的应用
US11673929B2 (en) Cyclized cytokines and method for producing same
US20250179445A1 (en) Recombinant kod polymerase
EP4541885A1 (en) Optimized phloroglucinol synthases
JP2018201508A (ja) グルタチオンストレス耐性酵母
WO2024211833A2 (en) Methods and compositions for nucleic acid synthesis
CN116355100A (zh) 一种碱基编辑器及其构建方法与应用
Akinbosede Structure-Function Studies of the ORF1 Protein From the Insertion-Site Specific Retroposon M5 Found in Indo-Pakistan Urban Malarial Vector Anopheles stephensi
WO2025099264A1 (en) Improved type v-k crispr-associated transposon mutants
HK40070059A (en) Ppr protein with less aggregation and use thereof
Mulaudzi An investigation of the zinc binding characteristics of the RING finger domain from the human RBBP6 protein using heteronuclear NMR spectroscopy.
CN107389945A (zh) 基于体外反应体系筛选黄曲霉sumo化靶蛋白的方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40080660

Country of ref document: HK