CN115671034A - Antibacterial, anti-inflammatory, itching-relieving, refreshing and comfortable cream for mosquito bites and preparation method thereof - Google Patents

Abstract

The invention provides an antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream for mosquito bites and a preparation method thereof, and the cream comprises the following components: peppermint extract, aizoon stonecrop herb extract, aloe extract, lemongrass extract, ginkgo leaf extract, geranium extract, berberine, evening primrose oil, turpentine oil, snake oil, coconut oil, composite emulsifier AC-402, glycerin, an ice crystal forming agent AVC, sodium alginate, sodium hyaluronate, 1,2-propylene glycol, methylparaben and distilled water. Compared with the prior art, the emulsifiable paste provided by the invention contains various natural active ingredients, takes berberine as a main drug, and exerts the effects of diminishing inflammation, relieving itching and calming together under the synergistic action of various active ingredients such as turpentine, evening primrose oil and the like. The cream provided by the invention is light yellow in color, appropriate in viscosity, fine and uniform in texture, bright in luster, fragrant and fresh in smell, and capable of effectively sterilizing and inhibiting bacteria, relieving itching, cooling and relieving and repairing skin.

Description

Antibacterial, anti-inflammatory, itching-relieving, refreshing and comfortable cream for mosquito bites and preparation method thereof

Technical Field

The invention belongs to the field of traditional Chinese medicines, and particularly relates to an antibacterial, anti-inflammatory, itching-relieving, cool and refreshing cream for mosquito bites and a preparation method thereof.

Background

Insect bite dermatitis is an acute inflammatory skin disease caused by insect bites or poison juice and poison burr, is a common skin disease in summer, and mainly shows local skin rash and skin pruritus as clinical manifestations. Insect dermatitis often affects people's daily life due to its high incidence and symptoms that cause skin itching. On the conventional treatment of insect bite dermatitis, patients with slight symptoms can be topically applied with antipruritic agents such as glucocorticoid preparations, calamine lotion, etc.; antihistamine can be taken orally by patients with extensive skin damage and serious symptoms; glucocorticoid medicaments can be taken orally for a short time for patients with severe anaphylaxis; the secondary infected person may be given an antibacterial drug.

At present, topical preparations for treating insect bite dermatitis on the market are common topical glucocorticoid creams, and are characterized by obvious anti-inflammatory effect, poor itching relieving effect and incapability of timely relieving pruritus of users. Moreover, hormone products are not conducive to growth and development in children. In addition, some antipruritic products in the market contain sensitizers such as essence and alcohol. It is possible that people allergic to such ingredients may develop an increased allergic response after insect bites upon use of such products. The cream is taken as a clinically common semi-solid external preparation for skin delivery, and is often taken as one of effective external preparations for treating inflammation caused by various inflammation factors. Therefore, a safe and reliable external cream with the functions of diminishing inflammation and relieving itching is urgently needed in the market at present.

Disclosure of Invention

The invention aims to provide an antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream for mosquito bites and a preparation method thereof.

The specific technical scheme of the invention is as follows:

an antibacterial, anti-inflammatory, antipruritic, refreshing and comfortable cream for mosquito bites comprises the following components in parts by weight:

the compound emulsifier is selected from a compound emulsifier AC-402.

The mass concentration of the sodium hyaluronate solution is 1%;

the aloe juice is prepared by the following method:

weighing appropriate amount of fresh Aloe pulp, placing into a juicer, adding 4-6 times of distilled water, squeezing for 3-5min in juicing mode to obtain juice, and filtering to obtain filtrate to obtain Aloe squeezed solution.

The mint extracting solution is prepared by the following method:

weighing appropriate amount of fresh folium Menthae, heating and reflux extracting with water for 3 times: adding water 18-20 times the weight of folium Menthae at 1 st time, soaking for 22-35min, and extracting for 1.5-2.0 hr; adding water 15-18 times the weight of folium Menthae at the 2 nd time, and extracting for 1.0-1.5 hr; adding 10-12 times of water by weight of folium Menthae at 3 rd time, and extracting for 0.5-1.0 hr; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to 6-10 times of the weight of folium Menthae at a volume of 6-10 mL/g to obtain herba Menthae extractive solution.

The volume of the mint extract liquid concentrated to 6-10 times of the mass of the mint leaves is 6-10mL per gram of fresh mint leaves extracted by the method.

The aizoon stonecrop herb extracting solution is prepared by the following method:

weighing cut aizoon stonecrop herb with proper amount, heating and refluxing with water for 3 times: soaking Notoginseng radix in 16-18 times of water for 16-38min on 1 st addition day, and extracting for 1.5-2.0 hr; extracting with 13-15 times of water for 1.0-1.5 hr; extracting Notoginseng radix with 8-12 times of water for 0.5-1.0 hr in 3 rd times of Jingjing day; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to 8-13 times of the mass of herba Sedi Aizoon to obtain herba Sedi Aizoon extractive solution.

The lemongrass extracting solution is prepared by the following method:

weighing appropriate amount of chopped lemongrass, heating and reflux-extracting with water for 3 times: adding water 20-22 times the weight of herba Imperatae in the 1 st time, soaking for 20-38min, and extracting for 1.5-2.0 hr; adding water 15-18 times the weight of the couch grass in the 2 nd time, and extracting for 1.0-1.5h; adding 10-13 times of water to the 3 rd time, and extracting for 0.5-1.0h; filtering each time to obtain filtrate, mixing filtrates, vacuum filtering, and concentrating the filtrate to volume of 8-12 times of the mass of the Cymbopogon Citrari (L.) Roxb/g to obtain Cymbopogon Citrari (L.) Roxb extract.

The ginkgo leaf extracting solution is prepared by the following method:

weighing appropriate amount of cut folium Ginkgo, and extracting with water under heating and refluxing for 3 times: adding water 18-20 times the weight of folium Ginkgo for the 1 st time, soaking for 18-38min, and extracting for 1.5-2.0 hr; adding 14-16 times of water by mass of folium Ginkgo for the 2 nd time, and extracting for 1.0-1.5 hr; adding water 10-12 times of folium Ginkgo for 3 times, and extracting for 0.5-1.0 hr; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to volume 10-14 times mL/g of folium Ginkgo to obtain folium Ginkgo extractive solution.

The geranium extracting solution is prepared by the following method:

weighing appropriate amount of minced geranium, heating and reflux-extracting with water for 3 times: adding water 20-22 times the weight of flos Pelargonii Hortori at the 1 st time, soaking for 12-30min, and extracting for 1.0-1.5 hr; adding water with the mass of 18-20 times of that of the pelargonium for the 2 nd time, and extracting for 0.75-1.0h; adding water 15-17 times the weight of flos Pelargonii Hortori in 3 rd time, and extracting for 0.3-0.5 hr; filtering each time, combining filtrates, performing suction filtration, and concentrating the volume of the filtrate to 2-7 times of the mass of the geranium (mL/g) to obtain the geranium extract for later use.

The invention provides a preparation method of an antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream for mosquito bites, which comprises the following steps:

a) Weighing 0.1-2.0g of ice crystal forming agent AVC, adding 24.0-60.0mL of distilled water, placing in a constant temperature water bath kettle at 70-80 deg.C, heating and stirring for 5-10min, standing at normal temperature for 6-18h to obtain ice crystal forming agent AVC gel liquid for use;

b) Weighing 0.2-2.0g of sodium alginate, adding 20.0-40.0mL of distilled water, heating and stirring in a constant temperature water bath kettle at 70-80 deg.C for 5-10min, standing at room temperature for 6-18h to obtain sodium alginate gel solution;

c) Weighing 0.15-0.45g of methylparaben, adding 1,2-propanediol 1.0-5.0mL, stirring and dissolving to obtain a preservative solution for later use;

d) Weighing and mixing 0.3-1.0g of evening primrose oil, 0.2-1.2g of turpentine, 0.3-1.0g of snake oil, 0.4-1.2g of coconut oil and 1.0-6.0g of compound emulsifier, placing in a constant-temperature water bath kettle at 70-90 ℃, and heating to obtain an oil phase for later use;

e) Weighing 6.0-10.0mL of distilled water in a beaker, placing in a constant-temperature water bath kettle at 70-90 ℃, and heating to obtain a water phase for later use;

f) When the oil phase and the water phase reach the same temperature, slowly adding the water phase into the oil phase in a trickle shape, heating while stirring at a constant speed in the same direction, stirring for 10-20min, and completing emulsification to obtain an O/W type emulsion matrix;

g) Weighing 0.5-8.0mL of aloe extract, 1.0-7.0mL of mint extract, 0.8-6.0mL of aizoon stonecrop extract, 1.0-8.0mL of lemongrass extract, 0.8-6.0mL of ginkgo leaf extract and 0.5-9.0mL of geranium extract, mixing, adding 0.01-0.05g of berberine and 0.1-0.5g of 1% sodium hyaluronate solution, and stirring uniformly to obtain a mixed solution A;

h) Adding the ice crystal forming agent AVC gel liquid, the sodium alginate gel liquid and the mixed liquid A into the O/W type emulsion matrix in the step f), and stirring to fully and uniformly mix the mixture to obtain a mixed system B;

i) Adding 2.0-5.0mL of glycerin and preservative solution into the mixed system B prepared in the step h), and stirring and mixing uniformly to obtain the antibacterial, anti-inflammatory, itching-relieving, cool and refreshing cream for mosquito bites.

In the preparation process, step a) forms an ice crystal forming agent AVC gel liquid; step b) forming sodium alginate gel liquid; step c) dissolving methyl paraben in transdermal absorption enhancer 1,2-propylene glycol to form a preservative solution; step d), weighing evening primrose oil, turpentine oil, snake oil, coconut oil and a composite emulsifier AC-402 to form an oil phase; step e) distilled water as the aqueous phase; step f) obtaining an O/W type emulsion matrix; step g) uniformly mixing various extracting solutions and functional components according to a certain proportion to form a mixed solution A; step h) fully utilizing the thickening capability of the gel matrix and the capability of the gel matrix for carrying water-soluble functional ingredients to form a preliminary emulsifiable paste preparation; step i) adding a humectant and a preservative to obtain the cream which is fine and uniform in texture and is used for inhibiting bacteria, diminishing inflammation, relieving itching, cooling and refreshing for mosquito bites.

The design principle of the invention is as follows:

aloe contains abundant aloin, emodin, and vitamin B ₆ And the like, and has the effects of resisting inflammation and relieving pain. Aloe tincture is antibacterial, and has effects in inhibiting inflammation, draining secretion, promoting local metabolism and cell regeneration, reducing scar formation, and enhancing disease resistance. Aloe also contains polysaccharide, has immunity enhancing effect, and contains wound hormone and polypeptide mannan as effective components for enhancing immunity and promoting wound healing. The aloe juice has the effects of cooling and moisturizing, and can fully retain the functional components of aloe by squeezing, thereby playing the characteristics of inflammation inhibition, antibiosis, nourishing and the like.

The mint is pungent in taste, cool in nature and nontoxic. The mint rhizome and leaf are rich in volatile oil, have cool fragrance, can treat symptoms such as skin rubella pruritus and measles imperviousness, and also have good curative effects on carbuncle, gangrene, scabies, tinea and dermatitis rhinis. The herba Menthae has effects of exciting central nerve, promoting glandular secretion, relieving inflammation, stimulating nerve ending cold receptor to produce cold feeling, and reflexively contracting deep tissue blood vessel to produce pain relieving and itching relieving effects. Volatile components such as peppermint oil and non-volatile components such as flavonoids, phenolic acids, quinones and triterpenes contained in the mint have the effects of inhibiting bacteria, resisting inflammation, diminishing swelling, softening tissues and the like, can effectively dispel wind, clear heat, detoxify and relieve itching, can bring cool and refreshing touch to the skin by using the mint, and can further relieve pain, itching and other troubles caused by mosquito bites. The menthol and borneol contained in the medicine also have the effects of cooling, relieving itching and reducing swelling. The effective components contained in the mint are more suitable to be dissolved in water, so that the extracting solution is obtained by adopting a water extraction mode, and the effects of cooling, relieving itching, resisting inflammation and reducing swelling can be fully exerted. The herba Menthae extractive solution has antiinflammatory, repercussive, analgesic, and antipruritic effects, and can be used as odor inhibitor, skin conditioner, aromatic, and antibacterial agent without acne in cosmetics, skin care products, and medicinal cream. The fragrant smell of the cream can also adjust the fragrance of the cream.

The herba Sedi Aizoon is root or whole plant of herba Senecionis Chrysanthemoidis of Compositae, has sweet taste, slight bitter taste and warm nature, and contains alkaloid, oleanolic acid, ursolic acid, flavonoids, herba Sedi Aizoon saccharide, fructose, protein, etc. as main ingredients. Aizoon stonecrop herb has the effects of stopping bleeding, dissipating blood stasis, relieving swelling and pain, clearing heat and removing toxicity and the like, is especially used for treating various hemorrhagic diseases, and is commonly used for treating symptoms such as traumatic injury, sores, carbuncle, deep-rooted boils, insect-snake bites and the like. The ursolic acid contained in aizoon stonecrop has various biological effects of sedation, anti-inflammation, antibiosis and the like, has obvious antioxidant function, and is widely used as a raw material of medicines and cosmetics. The root of the plant contains various alkaloids such as gynura segetum alkali, and has itching relieving effect. In addition, aizoon stonecrop herb not only has the inhibiting effect on staphylococcus aureus and pseudomonas aeruginosa, but also contains gallic acid which has various effects of resisting inflammation, mutation, oxidation and the like. The water extraction method can fully extract the effective components of total flavone, protocatechuic acid, vanillic acid, caffeic acid, ursolic acid and the like in the aizoon stonecrop herb, and the anti-inflammatory, bacteriostatic and detumescence effects of the aizoon stonecrop herb are achieved.

The lemongrass has lemon fragrance, can dispel wind-damp and dredge channels and collaterals, can treat symptoms such as rheumatic arthralgia and traumatic injury blood stasis, and has strong inhibiting effect on fungi due to citral contained in the lemongrass, also has the effect of killing fungi, and can effectively sterilize and diminish inflammation. Meanwhile, the smell of the lemongrass is not loved by insects, and the lemongrass can play a role in dispelling mosquitoes. The lemongrass water extract has good antibacterial effect, and the essential oil components contained in the lemongrass water extract have the effects of inhibiting or killing microorganisms and inhibiting gram-positive bacteria and gram-negative bacteria.

Folium Ginkgo is sweet and bitter in taste, and has effects of promoting blood circulation and dredging collaterals. The ginkgo leaf contains flavone which can inhibit the formation and deposition of pigment in the dermis, thereby achieving the effects of whitening skin and preventing and treating pigment plaques. Besides flavone, trace elements such as manganese and molybdenum in ginkgo can also remove oxygen free radicals, inhibit melanin generation and cell membrane lipid oxidation, and improve erythrocyte activity. Besides, ginkgo leaf can reduce blood viscosity and improve microcirculation system. The effective components of flavone and trace elements can be extracted by water extraction, and the effects of resisting oxidation and improving microcirculation system can be fully exerted.

The geranium is pungent in flavor and warm in nature, has the effects of dispelling wind, removing dampness, promoting qi circulation and relieving pain, and killing parasites, and has good curative effects on rheumatic arthralgia, hernia, eczema of scrotum, scabies and the like. The geranium can also enhance the function of lymphatic system, remove toxin and enhance autoimmunity. The contained plant essential oil can help to repair damaged skin cells and enhance the elasticity of the skin, has good effects on eczema, burn and frostbite, and enables pale and non-active skin to become red, moist and tender. The water extraction of the geranium is simple and easy to implement, has high efficiency, can reduce the loss of effective components compared with other extraction modes, and can fully exert the anti-inflammatory and antibacterial effects.

Berberine is cool in nature and can clear away pathogenic heat or deficiency heat and harmful substances. The berberine can inhibit pathogenic microorganism, and various bacteria such as dysentery bacillus, tubercle bacillus, pneumococcus, typhoid bacillus, and diphtheria bacillus, wherein the berberine has strongest effect on dysentery bacillus, and can be used for treating bacterial infection diseases. Berberine can inhibit the oxidation of pyruvic acid in the sugar metabolism process of bacteria, so that the bacteria can limit the utilization of vitamins, especially nicotinamide, to achieve the bacteriostatic effect, and inhibit the synthesis of DNA, RNA, protein and lipoid in the bacteria to interfere the reproduction of bacteria. The berberine can also inhibit acute and chronic inflammation, and has obvious inhibition effect on delayed hypersensitivity.

The evening primrose oil contains rich linoleic acid, has certain blood circulation promoting effect, and can improve platelet activity in human blood, prevent thrombosis, and promote blood circulation. It also contains multiple antioxidant components for preventing arteriosclerosis. The evening primrose oil has obvious nourishing effect on human skin, can promote the regeneration of skin cells and delay skin aging when being smeared on the surface of the skin, also can diminish inflammation, sterilize and resist viruses, and has obvious prevention and alleviation effects on the symptoms of eczema, dermatitis and the like which are common to human bodies.

Coconut oil has high stability and is not easy to oxidize, contains natural antibacterial medium chain fatty acid, has effects of killing bacteria, viruses, parasites and fungi, and can be used for treating scabies and neurodermatitis. The coconut oil is applied to skin diseases such as knife wound, scald, burn, mosquito bite and the like to accelerate wound healing without inflammation and leave no scar, and particularly has obvious curative effects on skin diseases such as diaper rash, rich hands, psoriasis and the like. The coconut oil also has the effects of maintaining beauty, keeping young and nourishing skin, and the lauric acid contained in the coconut oil can effectively improve the body immunity. Coconut oil can strengthen the contractile ability of pores, help to remove the outer dead skin of skin, repair damaged skin, make the junction of epidermis and dermis of skin healthier and smoother, generate new skin cells, improve dry and allergic skin, eliminate wrinkles and acne, and help skin keep away from chloasma and other skin blemishes caused by aging. Coconut oil has strong cleaning effect, can clean deep dirt of skin, and also has antioxidant and skin beautifying effects.

Turpentine is a skin irritant, has strong permeability, and can stimulate blood circulation, disinfect and clean skin. Turpentine also has strong antibacterial effect, has obvious inhibitory effect on Staphylococcus aureus or Escherichia coli, and can be used for treating tinea corporis, tinea pedis, etc. Meanwhile, the turpentine has the anti-inflammatory effect and also has better relieving and repairing effects on congestion, bleeding, mucosal necrosis and the like of some local tissues. The turpentine can effectively relieve muscle pain and treat arthralgia and neuralgia, and has effects of promoting blood circulation and relieving swelling when applied to sprain.

The snake oil is rich in trace elements and vitamins, and can not only moisten skin cells and relieve the symptom of dry skin, but also treat allergic symptoms and skin diseases caused by various bacterial infections. The snake oil is applied on skin, and has effects of maintaining moisture and elasticity of skin cells, delaying skin aging, preventing wrinkle, repairing skin cell injury, and caring skin. The snake oil can regulate hormone endocrine activity of human body, can be used for adjuvant treatment of skin tissue injury such as bacterial infection, bugantia, eczema, etc., and has effects of softening blood vessel, promoting blood circulation, eliminating blood stasis, sterilizing, and relieving swelling.

Sodium hyaluronate is a moisturizing factor with the strongest moisturizing property at present, and can repair skin injury, promote proliferation and differentiation of epidermal cells, clear oxygen free radicals and enhance the oxidation resistance of skin. The sodium hyaluronate has the function of moisturizing, and can also meet the requirements of skin on moisturizing in different seasons and different environments. In addition, the sodium hyaluronate has the wrinkle removing effect, and the small-molecule sodium hyaluronate can permeate into the dermis layer of the skin to promote the microcirculation of blood, facilitate the absorption of nutrients by the skin and enhance the elasticity and luster of the skin.

The glycerin can be used for moisturizing the whole body, and simultaneously can form a layer of protective film to prevent the evaporation and the dissipation of water on the surface layer of the skin, so that the skin can obtain better moisturizing effect, the skin can be effectively prevented from cracking, the skin is not dry, and the skin can be effectively repaired and maintained. Moreover, the protective film formed by smearing the glycerol on the wound surface can isolate microorganisms in the air from contacting with the skin, so that the possibility of infection of the wound surface is reduced, and the glycerol is mild in property, so that the glycerol does not stimulate the wound surface and has the effect of protecting the wound surface.

1,2-propylene glycol belongs to a micromolecule moisturizing component, can retain water in the stratum corneum, has low water absorption ratio, is generally used as a common hydrophilic moisturizing component in cleaning products, and can assist in dissolving dirt and avoid the rapid drying of the skin in the cleaning process. 1,2-propanediol is also a good skin penetration enhancer, and can promote the active ingredients in the cream to penetrate into the skin.

Sodium alginate is a natural polysaccharide, has strong hydrophilicity, and can form viscous uniform solution. The formed solution has high viscosity, stability and safety. Sodium alginate is a thickener with good performance and has a foam stabilizing effect. Meanwhile, the sodium alginate has good fluidity, is free from sticky feeling and stiff feeling, and gives people a more comfortable touch feeling. In addition, it has natural moisturizing effect, and can retain skin moisture to make skin tender and smooth.

The ice crystal former AVC is a synthetic polymer commonly used in gelling agents for clear systems and thickeners for oil-in-water emulsions to better stabilize the present emulsion matrix system. The polymer is convenient to use, good in dispersibility, fast to absorb, and excellent in stability given to the formula, and even good in stability without an emulsifier. The ice crystal forming agent AVC is light and thin in appearance, mild in property, low in viscosity and capable of bringing soft and comfortable touch to skin.

The compound emulsifier AC-402 is used as an intermediate bridge between water and oil, and is particularly suitable for preparing oil-in-water type cream. It can endow cream with excellent high and low temperature stability and fine and beautiful appearance. The composite emulsifier AC-402 has mild property, wide oil usability and strong emulsifying power, so that the antibacterial, anti-inflammatory, itching-relieving, cool and comfortable cream is easier to coat and has the function of enhancing skin feel. The viscosity of the emulsion prepared from the composite emulsifier AC-402 does not change along with time, and the emulsion can be compounded with various surfactants to generate a synergistic effect.

The methyl paraben has the capabilities of preventing fermentation, inhibiting bacterial proliferation and sterilizing, because the methyl paraben can passivate the activity of enzyme, inhibit the respiratory enzyme system and the transfer enzyme system of microbial cells and destroy the cell membrane structure of microbes, thereby having wide antibacterial action on molds, yeasts and bacteria. The antibacterial ability of the compound is stronger than that of sorbic acid and benzoic acid, the antibacterial effect is not greatly influenced by the pH value, and the effect is better in the range of the pH value between 4 and 8.

Herba Sedi Aizoon has effects of stopping bleeding, removing blood stasis, relieving swelling and pain, clearing heat and detoxicating; the herba Menthae has effects in exciting central nerve, promoting glandular secretion, and relieving inflammation; the lemon aldehyde contained in the lemongrass has strong effect of inhibiting the activity of fungi, and can effectively sterilize and diminish inflammation; berberine in berberine can inhibit acute and chronic inflammation; the evening primrose oil has the functions of diminishing inflammation, sterilizing and resisting viruses; the turpentine oil has effects of promoting blood circulation and relieving swelling; the snake oil has the effects of eliminating blood stasis, sterilizing and relieving swelling. The components complement each other and play the role of anti-inflammation and detumescence synergistically.

The mint is cool and cool, and can relieve the pain and the itching caused by mosquito bites; the aloe tincture contained in the aloe is a substance with strong antibacterial property, and can inhibit the growth of bacteria; the lemongrass water extract has good antibacterial effect; the berberine can resist pathogenic microorganisms; the coconut oil contains natural antibacterial components, and has effects of killing bacteria, viruses, parasites and fungi; turpentine has strong antibacterial effect; the snake oil can be used for treating various allergic symptoms and skin diseases caused by bacterial infection. The components supplement each other and play a role in inhibiting bacteria and relieving itching synergistically.

The aloe promotes local metabolism and promotes cell regeneration; the ginkgo leaves have good functions of promoting blood circulation, removing blood stasis and dredging collaterals; the geranium contains some plant essential oils which can help repair damaged skin cells and enhance the elasticity of the skin; evening primrose oil, coconut oil and snake oil have skin nourishing effect, and can promote skin cell regeneration and delay skin aging. The components interact with each other, so that the cream has the effects of promoting wound healing and repairing skin.

Compared with the prior art, the antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream for mosquito bites contains various natural active ingredients, and has the effects of diminishing inflammation, relieving itching and repairing skin under the synergistic effect of the various active ingredients such as berberine, turpentine, evening primrose oil and the like. Meanwhile, the cream is fresh and cool in use feeling and fragrant in smell, and can be used for treating dermatitis caused by mosquito bites, relieving itching symptoms, eliminating skin rash and enabling the skin to return to a healthy state.

Drawings

FIG. 1 is a microscope photograph (200 x) of the cream for mosquito bites, which is used for bacteriostasis, inflammation diminishing, itching relieving, cooling and refreshing;

FIG. 2 is a graph showing the irritation and allergy tests of the cream of the present invention;

FIG. 3 is a permeability test of the cream of the present invention;

FIG. 4 is a graph showing the change in the rate of swelling of the footpad of rats in each group according to the cream of the present invention;

FIG. 5 is a graph showing the effect of the cream of the present invention on the levels of NO, IL-1 β, IL-6 and TNF- α;

FIG. 6 is a graph showing the effect of the cream of the present invention on PGE2 content in rat inflamed feet;

FIG. 7 shows the variation of the itching frequency of the cream of the invention for each group of rats;

FIG. 8 is a photo of the bacteriostatic, anti-inflammatory, antipruritic, cooling and refreshing cream for mosquito bites prepared by the invention.

Detailed Description

The present invention will be further described with reference to the following examples.

According to the specification of pharmacopoeia, in the preparation process of the invention, the coarse powder can completely pass through a No. two sieve, but is mixed with powder which can pass through a No. four sieve by no more than 40%; all heat extractions were carried out under slightly boiling conditions.

Example 1

An antibacterial, anti-inflammatory, antipruritic, refreshing and comfortable cream for mosquito bites comprises the following components in parts by weight:

the aloe juice is prepared by the following method:

weighing appropriate amount of fresh Aloe pulp, placing into a juicer, adding 5 times of distilled water, squeezing for 4min in squeezing mode to obtain juice, and filtering to obtain filtrate to obtain Aloe squeezed solution.

The mint extracting solution is prepared by the following method:

weighing appropriate amount of fresh folium Menthae, heating and reflux extracting with water for 3 times: adding water 19 times the weight of folium Menthae at 1 st time, soaking for 25min, and extracting for 1.6 hr; adding 16 times of water by mass of folium Menthae at the 2 nd time, and extracting for 1.2 hr; adding 11 times of water by mass of folium Menthae at 3 rd time, and extracting for 0.50 hr; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to 7 times of the weight of folium Menthae at a volume of 7 mL/g to obtain herba Menthae extractive solution.

The aizoon stonecrop herb extracting solution is prepared by the following method:

weighing appropriate amount of cut herba Sedi Aizoon, extracting with water under heating and refluxing for 3 times: soaking Notoginseng radix in 17 times of water for 18min for 1 st time, and extracting for 1.6 hr; extracting with 15 times of water for 1.2 hr in 2 nd day; extracting with 10 times of water for 0.6 hr in 3 rd times of radix Notoginseng; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to volume 10 times mL/g of herba Sedi Aizoon to obtain herba Sedi Aizoon extractive solution.

The lemongrass extracting solution is prepared by the following method:

weighing appropriate amount of chopped lemongrass, heating and reflux-extracting with water for 3 times: adding 21 times of water by mass of couch grass in the 1 st time, soaking for 21min, and extracting for 1.5h; adding 16 times of water by mass of couch grass in the 2 nd time, and extracting for 1.1h; adding 11 times of water by mass of couch grass in the 3 rd time, and extracting for 0.6h; filtering each time to obtain filtrate, mixing the filtrates, vacuum filtering, and concentrating the filtrate to volume 9 times mL/g of the herba Cymbopogonis Citrari to obtain herba Cymbopogonis Citrari extractive solution for use.

The ginkgo leaf extracting solution is prepared by the following method:

weighing appropriate amount of cut folium Ginkgo, and extracting with water under heating and refluxing for 3 times: adding water 19 times the weight of folium Ginkgo at the 1 st time, soaking for 19min, and extracting for 1.5 hr; adding water 15 times the weight of folium Ginkgo for the 2 nd time, and extracting for 1.2 hr; adding 11 times of water by mass of folium Ginkgo for 3 times, and extracting for 0.6 hr; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to volume 12 times mL/g of folium Ginkgo to obtain folium Ginkgo extractive solution.

The geranium extracting solution is prepared by the following method:

weighing appropriate amount of minced geranium, heating and reflux extracting with water for 3 times: adding water 21 times the mass of flos Pelargonii Hortori at the 1 st time, soaking for 12min, and extracting for 1.1 hr; adding water 19 times the weight of flos Pelargonii Hortori at the 2 nd time, and extracting for 0.8 hr; adding water 16 times of the weight of the geranium in the 3 rd time, and extracting for 0.4h; filtering each time, combining filtrates, performing suction filtration, and concentrating the volume of the filtrate to 3 mL/g of the pelargonium mass to obtain pelargonium extract for use.

The invention provides a preparation method of an antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream for mosquito bites, which comprises the following steps:

a) Weighing an ice crystal forming agent AVC according to a formula ratio, adding 25.0mL of distilled water, putting into a constant-temperature water bath kettle at 75 ℃, heating and stirring for 6min, and standing for 11h at normal temperature to obtain an ice crystal forming agent AVC gel liquid for later use;

b) Weighing sodium alginate according to the formula ratio, adding 22.0mL of distilled water, placing into a constant-temperature water bath kettle at 75 ℃, heating and stirring for 6min, and standing for 7h at normal temperature to obtain sodium alginate gel liquid for later use;

c) Weighing nipagin methyl ester with the formula amount, adding 1,2-propylene glycol with the formula amount, stirring and dissolving to obtain a preservative solution for later use;

d) Weighing evening primrose oil, turpentine oil, snake oil, coconut oil and a compound emulsifier according to the formula ratio, mixing, placing in a constant-temperature water bath kettle at 75 ℃, and heating to obtain an oil phase for later use;

e) Measuring 6.0mL of distilled water in a beaker, placing the beaker in a water bath kettle with the constant temperature of 75 ℃, and heating the beaker to be used as a water phase for later use;

f) When the oil phase and the water phase reach the same temperature, slowly adding the water phase into the oil phase in a trickle shape, heating while uniformly stirring in the same direction, stirring for 12min, and completing emulsification to obtain an O/W type emulsion matrix;

g) Weighing and mixing the aloe extract, the mint extract, the aizoon stonecrop herb extract, the lemongrass extract, the ginkgo leaf extract and the geranium extract according to the formula amount, adding the berberine and the 1% sodium hyaluronate solution according to the formula amount, and uniformly stirring to obtain a mixed solution A;

h) Adding the ice crystal forming agent AVC gel liquid, the sodium alginate gel liquid and the mixed liquid A into the O/W type emulsion matrix in the step f), and stirring to fully and uniformly mix the mixture to obtain a mixed system B;

i) Adding the glycerol and the preservative solution with the formula amount into the mixed system B prepared in the step h), and stirring and mixing uniformly to obtain the antibacterial, anti-inflammatory, itching-relieving, cool and refreshing cream for mosquito bites.

Example 2

An antibacterial, anti-inflammatory, antipruritic, refreshing and comfortable cream for mosquito bites comprises the following components in parts by weight:

the aloe juice is prepared by the following method:

weighing appropriate amount of fresh Aloe pulp, placing into a juicer, adding 6 times of distilled water, squeezing for 5min in juicing mode to obtain juice, and filtering to obtain filtrate to obtain Aloe squeezed solution.

The mint extracting solution is prepared by the following method:

weighing appropriate amount of cut fresh folium Menthae, extracting with water under heating and refluxing for 3 times: adding water 20 times the weight of folium Menthae at 1 st time, soaking for 33min, and extracting for 2.0 hr; adding 16 times of water by mass of folium Menthae at the 2 nd time, and extracting for 1.3 hr; adding 11 times of water by mass of folium Menthae at 3 rd time, and extracting for 1.0 hr; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to 8 times of the weight of folium Menthae at a volume of 8 mL/g to obtain herba Menthae extractive solution.

The aizoon stonecrop herb extracting solution is prepared by the following method:

weighing appropriate amount of cut herba Sedi Aizoon, extracting with water under heating and refluxing for 3 times: soaking Notoginseng radix in 17 times of water for 18min for 2.0 hr on 1 st addition day; extracting with 15 times of water for 1.5 hr on day 2; extracting Notoginseng radix with 12 times of water for 0.8 hr on 3 rd additionnal day; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to volume 10 times mL/g of herba Sedi Aizoon to obtain herba Sedi Aizoon extractive solution.

The lemongrass extracting solution is prepared by the following method:

weighing appropriate amount of chopped lemongrass, heating and reflux-extracting with water for 3 times: adding 22 times of water by mass of couch grass in the 1 st time, soaking for 28min, and extracting for 1.5h; adding 15 times of water by mass of couch grass in the 2 nd time, and extracting for 1.5h; adding 13 times of water by mass of couch grass for the 3 rd time, and extracting for 1.0h; filtering each time to obtain filtrate, mixing the filtrates, vacuum filtering, and concentrating the filtrate to volume of 12 times mL/g of the mass of the Cymbopogon citratus to obtain Cymbopogon citratus extractive solution for use.

The ginkgo leaf extracting solution is prepared by the following method:

weighing appropriate amount of cut folium Ginkgo, and extracting with water under heating and refluxing for 3 times: adding water 20 times the weight of folium Ginkgo at 1 st time, soaking for 28min, and extracting for 2.0 hr; adding water 15 times the weight of folium Ginkgo for the 2 nd time, and extracting for 1.5 hr; adding water 12 times the weight of folium Ginkgo for the 3 rd time, and extracting for 1.0 hr; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to volume 12 times mL/g of folium Ginkgo to obtain folium Ginkgo extractive solution.

The geranium extracting solution is prepared by the following method:

weighing appropriate amount of minced geranium, heating and reflux extracting with water for 3 times: adding water 22 times the mass of flos Pelargonii Hortori at the 1 st time, soaking for 30min, and extracting for 1.5 hr; adding water 20 times the mass of flos Pelargonii Hortori at the 2 nd time, and extracting for 1.0h; adding water 17 times the weight of flos Pelargonii Hortori in the 3 rd time, and extracting for 0.4 hr; filtering each time, combining the filtrates, performing suction filtration, and concentrating the volume of the filtrate to 5 mL/g of the weight of the geranium to obtain a geranium extracting solution for later use.

The invention provides a preparation method of an antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream for mosquito bites, which comprises the following steps:

a) Weighing an ice crystal forming agent AVC according to a formula ratio, adding 50.0mL of distilled water, putting into a constant-temperature water bath kettle at 80 ℃, heating and stirring for 10min, standing at normal temperature for 12h to obtain an ice crystal forming agent AVC gel liquid for later use;

b) Weighing sodium alginate according to the formula ratio, adding 40.0mL of distilled water, placing into a constant-temperature water bath kettle at 80 ℃, heating and stirring for 10min, and standing at normal temperature for 18h to obtain sodium alginate gel liquid for later use;

c) Weighing nipagin methyl ester with the formula amount, adding 1,2-propylene glycol with the formula amount, stirring and dissolving to obtain a preservative solution for later use;

d) Weighing evening primrose oil, turpentine oil, snake oil, coconut oil and a compound emulsifier according to the formula ratio, mixing, placing in a constant-temperature water bath kettle at 80 ℃, and heating to obtain an oil phase for later use;

e) Measuring 10.0mL of distilled water in a beaker, placing the beaker in a water bath kettle with the constant temperature of 80 ℃, and heating the beaker to be used as a water phase for later use;

f) When the oil phase and the water phase reach the same temperature, slowly adding the water phase into the oil phase in a trickle shape, heating while uniformly stirring in the same direction, stirring for 12min, and completing emulsification to obtain an O/W type emulsion matrix;

g) Weighing and mixing aloe squeezing liquid, mint extracting liquid, aizoon stonecrop extracting liquid, lemongrass extracting liquid, ginkgo leaf extracting liquid and geranium extracting liquid according to a formula amount, adding berberine and 1% sodium hyaluronate solution according to the formula amount, and uniformly stirring to obtain mixed liquid A;

h) Adding the ice crystal forming agent AVC gel liquid, the sodium alginate gel liquid and the mixed liquid A into the O/W type emulsion matrix in the step f), and stirring to fully and uniformly mix the mixture to obtain a mixed system B;

i) Adding the formula amount of glycerol and the prepared preservative solution into the mixed system B prepared in the step h), and stirring and mixing uniformly to obtain the antibacterial, anti-inflammatory, itching-relieving, cool and refreshing cream for mosquito bites.

Example 3

An antibacterial, anti-inflammatory, antipruritic, refreshing and comfortable cream for mosquito bites comprises the following components in parts by weight:

the aloe juice is prepared by the following method:

weighing appropriate amount of fresh Aloe pulp, placing into a juicer, adding 6 times of distilled water, squeezing for 4min in juice squeezing mode to obtain juice, and filtering to obtain filtrate to obtain Aloe squeezed solution.

The mint extracting solution is prepared by the following method:

weighing appropriate amount of cut fresh folium Menthae, extracting with water under heating and refluxing for 3 times: adding water 20 times the weight of folium Menthae at 1 st time, soaking for 25min, and extracting for 2.0 hr; adding 18 times of water by mass of the mint leaves for the 2 nd time, and extracting for 1.5h; adding 12 times of water by mass of folium Menthae at 3 rd time, and extracting for 1.0 hr; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to 10 times of the weight of folium Menthae at a volume of 10 mL/g to obtain herba Menthae extractive solution.

The cut aizoon stonecrop herb extracting solution is prepared by the following method:

weighing proper amount of aizoon stonecrop herb, heating and refluxing with water for 3 times: soaking radix Notoginseng in water 18 times of the weight of radix Notoginseng in 1 st addition for 28min, and extracting for 1.8 hr; extracting with 15 times of water for 1.5 hr on day 2; extracting Notoginseng radix with 12 times of water for 1.0 hr in 3 rd times of Jingjing days; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to volume 12 times mL/g of herba Sedi Aizoon to obtain herba Sedi Aizoon extractive solution.

The lemongrass extracting solution is prepared by the following method:

weighing appropriate amount of chopped lemongrass, heating and reflux-extracting with water for 3 times: adding 22 times of water by mass of couch grass in the 1 st time, soaking for 33min, and extracting for 2.0h; adding 18 times of water by mass of couch grass in the 2 nd extraction for 1.5h; adding 13 times of water by mass of couch grass for the 3 rd time, and extracting for 1.0h; filtering each time to obtain filtrate, mixing the filtrates, vacuum filtering, and concentrating the filtrate to volume of 9 times mL/g of the mass of the Cymbopogon citratus to obtain Cymbopogon citratus extractive solution for use.

The ginkgo leaf extracting solution is prepared by the following method:

weighing appropriate amount of cut folium Ginkgo, and extracting with water under heating and refluxing for 3 times: adding 18 times of water by mass of folium Ginkgo at 1 st time, soaking for 30min, and extracting for 2.0 hr; adding 16 times of water by mass of folium Ginkgo for the 2 nd time, and extracting for 1.5 hr; adding water 12 times the weight of folium Ginkgo for the 3 rd time, and extracting for 1.0 hr; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to volume 12 times mL/g of folium Ginkgo to obtain folium Ginkgo extractive solution.

The geranium extracting solution is prepared by the following method:

weighing appropriate amount of minced geranium, heating and reflux extracting with water for 3 times: adding water 21 times the mass of flos Pelargonii Hortori at the 1 st time, soaking for 16min, and extracting for 1.5 hr; adding water 19 times the weight of flos Pelargonii Hortori at the 2 nd time, and extracting for 1.0h; adding water 16 times of the weight of the geranium in the 3 rd time, and extracting for 0.4h; filtering each time, combining filtrates, performing suction filtration, and concentrating the volume of the filtrate to 5 mL/g of the pelargonium mass to obtain pelargonium extract for use.

The invention provides a preparation method of an antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream for mosquito bites, which comprises the following steps:

a) Weighing an ice crystal forming agent AVC according to a formula ratio, adding 40.0mL of distilled water, putting into a constant-temperature water bath kettle at 70 ℃, heating and stirring for 8min, and standing for 11h at normal temperature to obtain an ice crystal forming agent AVC gel liquid for later use;

b) Weighing sodium alginate according to the formula ratio, adding 40.0mL of distilled water, placing into a constant-temperature water bath kettle at 70 ℃, heating and stirring for 10min, and standing at normal temperature for 12h to obtain sodium alginate gel liquid for later use;

c) Weighing nipagin methyl ester with the formula amount, adding 1,2-propylene glycol with the formula amount, stirring and dissolving to obtain a preservative solution for later use;

d) Weighing evening primrose oil, turpentine oil, snake oil, coconut oil and a compound emulsifier according to the formula ratio, mixing, placing in a constant-temperature water bath kettle at 80 ℃, and heating to obtain an oil phase for later use;

e) Measuring 10.0mL of distilled water in a beaker, placing the beaker in a water bath kettle with the constant temperature of 80 ℃, and heating the beaker to be used as a water phase for later use;

f) When the oil phase and the water phase reach the same temperature, slowly adding the water phase into the oil phase in a trickle shape, heating while stirring at a constant speed in the same direction, stirring for 16min, and completing emulsification to obtain an O/W type emulsion matrix;

g) Weighing and mixing aloe squeezing liquid, mint extracting liquid, aizoon stonecrop extracting liquid, lemongrass extracting liquid, ginkgo leaf extracting liquid and geranium extracting liquid according to a formula amount, adding berberine and 1% sodium hyaluronate solution according to the formula amount, and uniformly stirring to obtain mixed liquid A;

h) Adding the ice crystal forming agent AVC gel liquid, the sodium alginate gel liquid and the mixed liquid A into the O/W type emulsion matrix in the step f), and stirring to fully and uniformly mix the mixture to obtain a mixed system B;

i) Adding the formula amount L of glycerol and the preservative solution into the mixed system B prepared in the step h), and stirring and mixing uniformly to obtain the antibacterial, anti-inflammatory, itching-relieving, cool and refreshing cream for mosquito bites.

Example 4 (for comparison)

Otherwise, as in example 1, significant precipitation occurred with only a small addition of U30 cellulosic thickener, indicating that U30 cellulosic thickener is not suitable for this formulation. The product was not subjected to subsequent experiments.

Example 5 (for comparison)

Otherwise, as in example 2, only seabuckthorn oil was substituted for evening primrose oil, and the resulting cream had a strong taste and was generally regarded by the experimenter as unacceptable. The product was not subjected to subsequent experiments.

Example 6

The physical and chemical indexes of the examples 1,2 and 3 are as follows:

6.1 Properties

The cream for resisting bacteria, diminishing inflammation, relieving itching, cooling and refreshing for mosquito bites prepared by the invention is a faint yellow cream, has proper viscosity, fine and uniform texture, bright and moist appearance and fresh smell, and the product picture is shown in figure 8.

6.2pH check

The cream for inhibiting bacteria, diminishing inflammation, relieving itching, cooling and refreshing for mosquito bites prepared in the embodiments 1,2 and 3 is measured by pH test paper, and the measured pH value is 6-7.

6.3 Cold-Heat test

The cream for inhibiting bacteria, diminishing inflammation, relieving itching, cooling and refreshing for mosquito bites prepared in the embodiments 1,2 and 3 is packaged in a cream bottle, and is refrigerated in a refrigerator at 4 ℃ for one month to observe no layering phenomenon; the cream is placed in a constant temperature box at 55 ℃ for 24 hours, and the phenomena of layering, emulsification, taste change and the like of the cream are avoided.

6.4 centrifugation test

The cream prepared in the embodiments 1,2 and 3 for mosquito bites with the functions of bacteriostasis, anti-inflammation, relieving itching, cooling and refreshing is packaged in a test tube with a plug and centrifuged at 3000r/min for 20min, and no layering phenomenon occurs.

6.5 Room temperature standing test

The cream for mosquito bites, which is prepared in the embodiments 1,2 and 3, has the functions of bacteriostasis, inflammation diminishing, itching relieving, cooling and refreshing, is filled in a cream bottle, is stood at room temperature for 6 months, has no layering phenomenon, and has no change in the feeling after use and no change in the smell.

6.6 Observation of emulsion droplets

The size and uniformity of the emulsion droplets of the cream are judged by observation under an OLYMPUS microscope. The results show that the emulsion droplets are uniform and moderate in size, see fig. 1.

6.7 irritation and allergy test

Shearing hair on the back of mice, applying the cream for resisting bacteria, diminishing inflammation, relieving itching, cooling and refreshing for mosquito bite, observing after 30min, and comparing with the uncoated part to obtain the product without irritation and anaphylaxis.

A proper amount of the antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream for mosquito bites prepared in the examples 1,2 and 3 is applied to the hand surface of a volunteer (20-73 years old and 60 people), and after 30min, the cream is observed and compared with the uncoated part, and no redness, eruption or foaming phenomenon appears. The experiment is shown in FIG. 2, the left side is a white mouse experiment, and the right side is a volunteer hand experiment.

6.8 Permeability test

Taking agar powder, preparing a 1% aqueous solution according to a proportion, and adding 8% ferric ammonium sulfate solution as a color developing agent when the agar powder is in a liquefied state. 2 test tubes were taken and added with the appropriate amount of agar solution prepared above. The same amount of the cream for inhibiting bacteria, diminishing inflammation, relieving itching, cooling and refreshing for mosquito bites and the reference substance (polysaccharide polysulfonate cream, batch number: 202104) are respectively filled in the gap of 40mm at the upper end. The height of the colored areas was observed and measured at 1,2, 4, 8, 16 and 24 h. From Lockie empirical formula Y ² K value is obtained, and the release rate of the drug from the matrix can be judged according to the K value, and the result is shown in fig. 3. And performing linear regression on the square of the average diffusion distance (Y) and the diffusion time (X) to obtain a regression equation. And (5) obtaining the K value and the correlation coefficient R. As a result, the cream prepared according to the invention K =0.5213 ² =0.9552. Control K =0.2707,r ² =0.9702. The K =0.5213 of the cream prepared is greater than the control K =0.2707. The permeability of the prepared antibacterial, anti-inflammatory, itching-relieving, cool and refreshing emulsifiable paste is superior to that of the mucopolysaccharide polysulfonate emulsifiable paste.

6.9 cream anti-inflammatory and repercussive experiment

SD rats (SD rats with weight difference not more than 10 g) are randomly divided into a blank group (KB), a model group (MX) and an antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream (RG) group for mosquito bites, 6 rats are respectively arranged in each group, the RG group is fixed on the unhaired back every day and is smeared with the antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream (2 g) once, and is continuously smeared for 7 days, and no medicament is smeared in the blank group (KB) and the model group (MX). In RG group, 0.1mL of a 1% carrageenan solution was injected subcutaneously into the plantar aspect of the right hind paw of the rat at 0.5h after administration, resulting in inflammation on day 7. In MX group, 0.1mL of a 1% carrageenan solution was injected subcutaneously into the plantar region of the right hind paw of the rat to cause inflammation, and in KB group, 0.1mL of physiological saline was injected subcutaneously into the plantar region of the right hind paw of the rat.

6.9.1 Effect on swelling rate of feet in swollen rats

The swelling rate was calculated by measuring the thickness of the plantar surface of the right hind paw after the onset of inflammation with a vernier caliper at 0.5, 1.5, 2.0, 3.0 and 5.0h after the onset of inflammation. Swelling ratio (%) = (thickness measured at a certain time point after molding-thickness before molding)/thickness before molding × 100%. The results are shown in FIG. 4. ^## p<Kb set 0.01vs; ^$$ p<mx group 0.01vs.

As can be seen in fig. 4, the swelling of the footpad of the rats at each time point in MX group was significantly increased (p < 0.01) compared to KB group, indicating successful modeling. Compared with the MX group, the RG group can reduce the increase of the foot swelling rate of a rat foot swelling model induced by carrageenan to a certain degree at each time point (p is less than 0.01), and the cream for inhibiting bacteria, diminishing inflammation, relieving itching, cooling and refreshing for mosquito bites can be preliminarily judged to have a certain inhibiting effect on the rat foot swelling induced by the carrageenan.

6.9.2 Effect on serum NO, IL-1 beta, TNF-alpha and IL-6 levels

After the thickness of the metatarsus of the foot is measured for the last time, the rat is weighed, a 25% urethane solution is injected into the abdominal cavity according to the weight of 0.4mL/100g, the rat is anesthetized, the eyeball is removed, blood is collected, the blood is centrifuged at high speed for 15min at 4 ℃ and 3500r/min, and the supernatant is taken to obtain the serum. The content of NO, IL-1 beta, TNF-alpha and IL-6 in serum is determined by adopting an NO kit, an IL-1 beta ELISA kit, a TNF-alpha ELISA kit and an IL-6ELISA kit according to the kit specification. The results are shown in figure 5 which shows, ^## p<kb set 0.01vs; ^$$ p<mx group 0.01vs.

As can be seen from FIG. 5, the level of expression of NO, cytokines IL-1. Beta., IL-6 and TNF-. Alpha.was significantly increased in MX group relative to KB group (p < 0.01), indicating successful modeling. Compared with the MX group, the RG group can obviously reduce the content of NO, cell factors IL-1 beta, IL-6 and TNF-alpha in the supernate (p is less than 0.01), which shows that the bacteriostatic, anti-inflammatory, antipruritic, refreshing and comfortable cream for mosquito bites has an anti-inflammatory effect.

6.9.3 rat inflammatory paw ₂ Influence of the amount

After dislocation and death, sequentially cutting inflammatory feet at the same positions of ankle joints of each group of rats, cutting soft tissues in the inflammatory feet, weighing, cutting into pieces, fully immersing in quantitative physiological saline for 1h, centrifuging at 4 ℃ at 3000rpm, uniformly mixing 0.2mL of supernatant with 0.5mol/L KOH-methanol solution, carrying out water bath reaction at 50 ℃ for 20min, diluting to 5mL by methanol, measuring the absorbance of the solution under the condition of 278nm wavelength, wherein the content is represented by the absorbance of inflammatory tissues multiplied by 100/g. The results are shown in figure 6 which shows, ^## p<kb set 0.01vs; ^$$ p<mx group 0.01vs.

As can be seen from FIG. 6, MX group rats were PGE under the action of carrageenan compared with KB group ₂ The content is obviously increased (p)<0.01). RG group PGE compared to MX ₂ The content is obviously reduced (p)<0.01)。

The experiments show that the prepared cream for inhibiting bacteria, diminishing inflammation, relieving itching, cooling and refreshing for mosquito bites can effectively inhibit NO, cell factors IL-1 beta and TNF-Alpha, IL-6 and PGE in inflammatory feet ₂ Content, and has good anti-inflammatory effect.

6.10 investigation of itching relieving effect of the antibacterial, anti-inflammatory, itching relieving, refreshing and refreshing cream for mosquito bites

Another 18 SD rats were randomly divided into KB, MX and RG groups, each of 6. RG group applied antibacterial, anti-inflammatory, antipruritic, refreshing and refreshing cream to the unhaired part of rat back once a day, 2g cream for each time, and 7 days for continuous application. On day 7 after the application, 1h, KB groups were injected subcutaneously into the back with 0.1mL of physiological saline, and the remaining two groups were injected subcutaneously into the back with 0.1mL of 0.001g/mL histamine solution. The number of times is taken as a calculation unit, the front paw of the rat scratches the head, the rear paw scratches the trunk, and the mouth licks each part to be taken as pruritus once, and the total number of times of pruritus attack within 10min is recorded. The results are shown in figure 7 of the drawings, ^## p<kb set 0.01vs; ^$$ p<mx group 0.01vs.

According to the figure 7, the number of the MX group pruritus is obviously increased compared with that of the KB group (p is less than 0.01), and the number of the RG group pruritus is obviously reduced compared with that of the MX group (p is less than 0.01), so that the antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream for mosquito bites can inhibit pruritus caused by histamine solution and has certain antipruritic effect.

6.11 examination of the bacteriostatic Effect

In the same laboratory, the common emulsifiable paste without anti-inflammatory and bactericidal components prepared on the same day and the antibacterial, anti-inflammatory, antipruritic, cool and comfortable emulsifiable paste for mosquito bites prepared according to the embodiment 3 are examined under the condition that no preservative is added, (36 +/-1 ℃) sample retention, and the result shows that the common emulsifiable paste has rancidity conditions such as odor, foam and the like within 36h, while the emulsifiable paste of the embodiment 3 still has no rancidity conditions such as odor, foam and the like after being placed for two weeks, and the trial feeling and smell have no obvious changes, thereby fully showing that the antibacterial, anti-inflammatory, antipruritic, cool and comfortable emulsifiable paste has certain antibacterial effect.

The preparation method of the common cream comprises the following steps: the oil phase is 5g of rice oil, the emulsifier is 5g of olive oil emulsified wax, and the water phase is 20ml of distilled water, and the preparation method comprises the following steps: slowly adding water phase into the oil phase along the wall of the container when the oil phase and water phase reach the same temperature of 80 deg.C in 80 deg.C constant temperature water bath, heating and stirring at the same direction at constant speed for 30min, and emulsifying to obtain O/W type emulsion cream.

6.12 examination of comprehensive effects of antibacterial, anti-inflammatory, antipruritic, refreshing and comfortable emulsifiable paste

The study has been approved by the southern Anhui medical college ethics Committee and was conducted under its direction. The efficacy of the bacteriostatic, anti-inflammatory, antipruritic, cooling and refreshing creams for mosquito bites prepared in examples 1,2 and 3 was evaluated as shown in table 1. A folk survey grading method is adopted, volunteers (20-73 years old, 60 people in total) are selected as trial objects, the trial objects are divided into three groups at random, the antibacterial, anti-inflammatory, itching-relieving, cool and refreshing emulsifiable paste of the three embodiments is respectively applied to the bite, itching or allergic part of a mosquito bite, 3 times a day and the using time is till the symptoms of the affected part disappear, and the comprehensive effect of the antibacterial, anti-inflammatory, itching-relieving, cool and refreshing emulsifiable paste is investigated and shown in Table 1. The using time is from 2021 year, 5 months and 13 days to 2021 year, 7 months and 13 days, and the using effect is divided into 5 points in total: the score of 5 is the highest score, which represents good and very satisfactory; 4, better score is given; 3 is acceptable; when the amount is less than 3 points, the results are not acceptable. The average score of each item is as follows.

Table 1 comprehensive effect investigation

In conclusion, the antibacterial, anti-inflammatory, antipruritic, cool and refreshing cream for mosquito bites has good anti-inflammatory, detumescence and antipruritic effects. In addition, the cream provided is fine and uniform in texture, bright in luster and fragrant and fresh in smell, and can effectively sterilize and inhibit bacteria, relieve itching, cool and relieve, and repair skin.

6.14 clinical trials

The study was approved by the ethical committee of southern anhui medical college and was conducted under its direction. Observing an object: the cream of the invention is used for treating 50 patients with mosquito bites. The observations were as follows: and (3) diagnosis: the mosquito bite part has red rash, red swelling, blister and local pruritus. The use method comprises the following steps: applied directly, 3 times daily. The treatment time is about 3 days. The therapeutic effect judgment standard is as follows: and (3) curing: clinical symptoms all disappeared; the effect is shown: the clinical symptoms mostly disappear; and (4) invalidation: the clinical symptoms did not disappear or worsen, and the experimental results are shown in table 2.

TABLE 2 Effect of use

The results show that: the cream prepared by the invention is used by clinical patients, and has quick response and good treatment effect.

Claims (10)

1. The antibacterial, anti-inflammatory, antipruritic, cool and comfortable cream for mosquito bites is characterized by comprising the following components in parts by weight:

2. the cream for mosquito bite with antibacterial, anti-inflammatory, antipruritic, cooling and refreshing effects as claimed in claim 1, wherein the compound emulsifier is selected from a compound emulsifier AC-402.

3. The cream for mosquito bites, which is antibacterial, anti-inflammatory, antipruritic, cool and refreshing according to claim 1, wherein the mass concentration of the sodium hyaluronate solution is 1%.

4. The cream for mosquito bites, which is antibacterial, anti-inflammatory, antipruritic, cool and refreshing according to claim 1 or 2, characterized in that the aloe juice is prepared by the following method:

weighing appropriate amount of fresh Aloe pulp, placing into a juicer, adding 4-6 times of distilled water, squeezing for 3-5min in juicing mode to obtain juice, and filtering to obtain filtrate to obtain Aloe squeezed solution.

5. The antibacterial, anti-inflammatory, antipruritic, refreshing and comfortable cream for mosquito bites according to claim 1 or 2, characterized in that the mint extract is prepared by the following method:

weighing appropriate amount of cut fresh folium Menthae, extracting with water under heating and refluxing for 3 times: adding water 18-20 times the weight of folium Menthae at 1 st time, soaking for 22-35min, and extracting for 1.5-2.0 hr; adding water 15-18 times the weight of folium Menthae at the 2 nd time, and extracting for 1.0-1.5 hr; adding 10-12 times of water by weight of folium Menthae at 3 rd time, and extracting for 0.5-1.0 hr; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to 6-10 times of the weight of folium Menthae at a volume of 6-10 mL/g to obtain herba Menthae extractive solution.

6. The cream for mosquito bites, which is antibacterial, anti-inflammatory, antipruritic, cool and refreshing according to claim 1 or 2, is characterized in that the aizoon stonecrop herb extracting solution is prepared by the following method:

weighing cut aizoon stonecrop herb with proper amount, heating and refluxing with water for 3 times: soaking Notoginseng radix in 16-18 times of water for 16-38min on 1 st addition day, and extracting for 1.5-2.0 hr; extracting with 13-15 times of water for 1.0-1.5 hr; extracting with 8-12 times of water for 0.5-1.0 hr in 3 rd adding days; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to 8-13 times of the mass of herba Sedi Aizoon to obtain herba Sedi Aizoon extractive solution.

7. The cream for mosquito bite inhibition, inflammation diminishing, itching relieving, cooling and refreshing as claimed in claim 1 or 2, wherein the lemongrass extract is prepared by the following method:

weighing appropriate amount of chopped lemongrass, heating and reflux-extracting with water for 3 times: soaking in water 20-22 times the mass of herba Imperatae in 1 st time for 20-38min, and extracting for 1.5-2.0h; adding water 15-18 times the weight of the couch grass in the 2 nd time, and extracting for 1.0-1.5h; adding 10-13 times of water to the 3 rd time, and extracting for 0.5-1.0h; filtering each time to obtain filtrate, mixing filtrates, vacuum filtering, and concentrating the filtrate to volume of 8-12 times of the mass of the Cymbopogon Citrari (L.) Roxb/g to obtain Cymbopogon Citrari (L.) Roxb extract.

8. The cream for mosquito bite inhibition, inflammation diminishing, itching relieving, cooling and refreshing as claimed in claim 1 or 2, wherein the ginkgo leaf extract is prepared by the following method:

weighing appropriate amount of cut folium Ginkgo, and extracting with water under heating and refluxing for 3 times: adding water 18-20 times the weight of folium Ginkgo at the 1 st time, soaking for 18-38min, and extracting for 1.5-2.0h; adding 14-16 times of water by mass of folium Ginkgo for the 2 nd time, and extracting for 1.0-1.5 hr; adding water 10-12 times of folium Ginkgo for 3 times, and extracting for 0.5-1.0 hr; filtering each time, mixing filtrates, vacuum filtering, and concentrating the filtrate to volume 10-14 times mL/g of folium Ginkgo to obtain folium Ginkgo extractive solution.

9. The cream for mosquito bite inhibition, inflammation diminishing, itching relieving, cooling and refreshing as claimed in claim 1 or 2, wherein the geranium extract is prepared by the following method:

weighing appropriate amount of minced geranium, heating and reflux extracting with water for 3 times: adding water 20-22 times the weight of flos Pelargonii Hortori at the 1 st time, soaking for 12-30min, and extracting for 1.0-1.5 hr; adding water with the mass of 18-20 times of that of the pelargonium for the 2 nd time, and extracting for 0.75-1.0h; adding water 15-17 times the weight of flos Pelargonii Hortori in 3 rd time, and extracting for 0.3-0.5 hr; filtering each time, combining filtrates, performing suction filtration, and concentrating the volume of the filtrate to 2-7 times of the mass of the geranium (mL/g) to obtain the geranium extract for later use.

10. A method for preparing the cream for mosquito bite inhibition, inflammation diminishing, itching relieving, cooling and refreshing as claimed in any one of claims 1 to 9, wherein the preparation method comprises the following steps:

a) Weighing 0.1-2.0g of ice crystal forming agent AVC, adding 24.0-60.0mL of distilled water, placing in a constant temperature water bath kettle at 70-80 deg.C, heating and stirring for 5-10min, standing at normal temperature for 6-18h to obtain ice crystal forming agent AVC gel liquid for use;

b) Weighing 0.2-2.0g of sodium alginate, adding 20.0-40.0mL of distilled water, heating and stirring in a constant temperature water bath kettle at 70-80 deg.C for 5-10min, standing at room temperature for 6-18h to obtain sodium alginate gel solution;

c) Weighing 0.15-0.45g of methylparaben, adding 1,2-propanediol 1.0-5.0mL, stirring and dissolving to obtain a preservative solution for later use;

d) Weighing and mixing 0.3-1.0g of evening primrose oil, 0.2-1.2g of turpentine, 0.3-1.0g of snake oil, 0.4-1.2g of coconut oil and 1.0-6.0g of compound emulsifier, placing in a constant-temperature water bath kettle at 70-90 ℃, and heating to obtain an oil phase for later use;

e) Weighing 6.0-10.0mL of distilled water in a beaker, placing in a constant-temperature water bath kettle at 70-90 ℃, and heating to obtain a water phase for later use;

f) When the oil phase and the water phase reach the same temperature, slowly adding the water phase into the oil phase in a trickle shape, heating while stirring at a constant speed in the same direction, stirring for 10-20min, and completing emulsification to obtain an O/W type emulsion matrix;

g) Weighing 0.5-8.0mL of aloe squeeze liquid, 1.0-7.0mL of mint extract, 0.8-6.0mL of aizoon stonecrop extract, 1.0-8.0mL of lemongrass extract, 0.8-6.0mL of ginkgo leaf extract and 0.5-9.0mL of geranium extract, mixing, adding 0.01-0.05g of berberine and 0.1-0.5g of 1% sodium hyaluronate solution, and stirring uniformly to obtain mixed solution A;

h) Adding the ice crystal forming agent AVC gel liquid, the sodium alginate gel liquid and the mixed liquid A into the O/W type emulsion matrix in the step f), and stirring to fully and uniformly mix the mixture to obtain a mixed system B;

i) Adding 2.0-5.0mL of glycerin and preservative solution into the mixed system B prepared in the step h), and stirring and mixing uniformly to obtain the antibacterial, anti-inflammatory, itching-relieving, cool and refreshing cream for mosquito bites.

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