CN115669533A - Breeding method for new green wheat variety - Google Patents
Breeding method for new green wheat variety Download PDFInfo
- Publication number
- CN115669533A CN115669533A CN202211404499.3A CN202211404499A CN115669533A CN 115669533 A CN115669533 A CN 115669533A CN 202211404499 A CN202211404499 A CN 202211404499A CN 115669533 A CN115669533 A CN 115669533A
- Authority
- CN
- China
- Prior art keywords
- wheat
- generation
- highland barley
- breeding method
- planting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to the technical field of agriculture and wheat breeding, and discloses a breeding method of a new green wheat variety, wherein S1: selecting highland barley as male parent and wheat as female parent; s2: placing highland barley in a vacuum closed environment, performing radiation treatment on the highland barley, placing the highland barley in a land for planting, and performing individual plant selection according to the yield; s3: carrying out high-temperature stress on wheat seeds to plant wheat female parents, and carrying out single plant selection according to high-temperature resistance after 8-10 times of high-temperature stress treatment; s4: carrying out hybridization cultivation on two single plants selected from the S2 and the S3, and selecting a high-yield single plant to form an F1 generation; s5: and hybridizing the excellent single plants of the F1 generation with the highland barley male parents again to finish the F2 generation cultivation. The method is favorable for cultivating green wheat varieties with higher comprehensiveness, and combines cold resistance and high temperature resistance, so that the wheat planting latitude is greatly increased, and the method is also suitable for planting in the land with larger temperature difference.
Description
Technical Field
The invention relates to the technical field of agriculture and wheat breeding, in particular to a breeding method of a new green wheat variety.
Background
The highland barley is also called naked barley and highland barley, is annual herbaceous plant of gramineae and barley, is highland cereal crop, has strong cold resistance, short growth period, high yield, early maturity and wide adaptability, is wheat which is gramineae plant and is a cereal crop widely planted all over the world, and the caryopsis of wheat is one of staple food of human beings and can be used for preparing foods such as bread, steamed bread, biscuits, noodles and the like after being ground into flour.
The invention relates to the existing Chinese patent (publication number: CN 112493118B) and a breeding method of multi-spike and multi-grain spring wheat, which aims to improve the grain number of the spring wheat, realizes high yield by reasonable close planting, and utilizes Tibet fingered citron wheat, chinese 2 spike branch winter wheat and spring wheat as parents to carry out sexual hybridization, winter wheat meets the flower period of the spring wheat by an early method, and the spring multi-spike and multi-grain wheat is selected by systematic breeding and selection by methods of test cross, compound cross, backcross, winter generation addition and the like, the grain number of the cultivated wheat variety per plant is improved to 150-300 grains, the average tillering and ear forming of each plant is 1.5-3, the average single spike weight is 4-6 g, and the thousand grain weight reaches 33-48 g; the wheat yield is improved by increasing the grain number per ear, the yield per mu is more than 480 kg, the yield per mu is increased by more than 20% compared with the yield per mu of 400 kg of the current common wheat Tongmai No. 2, the yield per mu of the wheat is greatly improved, for China, the planting area of the wheat is mostly concentrated in middle areas such as Hebei, shanxi, henan, shandong, anhui, hubei, jiangsu, sichuan, shaanxi and the like, the latitude of wheat planting is not improved, and the wider soil in the North is lack of suitable crops.
Therefore, the new green wheat variety breeding method is characterized in that excellent high-yield single plants of highland barley are selected through radiation treatment, excellent high-temperature-resistant single plants of wheat are selected through high-temperature stress treatment, two kinds of hybrid cultivation are carried out, backcross stable cultivation is carried out simultaneously, disease resistance and cold resistance detection are carried out every generation, high-quality single plants are selected for secondary cultivation, protein content of the high-quality single plants is detected, the single plants with better quality are selected for cultivation, the green wheat variety with higher comprehensive performance is cultivated, and the planting latitude of wheat is greatly increased by combining the cold resistance and the high temperature resistance, and meanwhile, the method can be suitable for planting in the land with larger temperature difference.
Disclosure of Invention
The invention aims to provide a breeding method of a new green wheat variety, which solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a breeding method of a new green wheat variety, S1: selecting a highland barley variety as a male parent and a wheat variety as a female parent;
s2: placing highland barley in a vacuum closed environment, performing radiation treatment on the highland barley, placing the highland barley in a land for planting, and performing individual plant selection according to the yield;
s3: carrying out high-temperature stress on wheat seeds to plant wheat female parents, and carrying out single plant selection according to high-temperature resistance after 8-10 times of high-temperature stress treatment;
s4: carrying out hybridization cultivation on two single plants selected from the S2 and the S3, and selecting a high-yield single plant to form an F1 generation;
s5: hybridizing the excellent single plants of the F1 generation with the highland barley male parents again to finish the cultivation of the F2 generation;
s6: hybridizing the F2 generation excellent single plant with the wheat female parent again to finish F3 generation culture
S7: hybridizing the excellent F3 generation single plants with the F1 band again to finish F4 generation cultivation;
s8: planting F4 generations in multiple regions, and selecting high-yield high-temperature-resistant excellent single plants to form F5 generations;
s9: and planting the F5 generation for multiple generations, and identifying the F5 generation.
In a preferred embodiment of the invention, kunlun No. 10 is adopted as highland barley, and Zhongmai 895 is adopted as wheat.
In a preferred embodiment of the present invention, the highland barley seeds are treated with non-ionizing radiation at a treatment power of 60W for 30 seconds.
As a preferred embodiment of the invention, the germination rate of the highland barley is recorded after the highland barley is planted, and the individual plants with high yield, large number of grains and high quality are selected.
As a preferred embodiment of the invention, wheat needs to select the individual plant with the optimal green leaf area ratio for selection after high-temperature stress.
In a preferred embodiment of the present invention, both of S2 and S3 are treated at room temperature, and then subjected to liquid chromatography to detect the protein content, preferably selecting the individual.
In a preferred embodiment of the invention, emasculation and pollination are adopted in S5, so that the weak and strong retention are observed, the stem and leaf are not damaged, and the bag is required to be sleeved and the label is attached after pollination is finished.
As a preferred embodiment of the present invention, during the cultivation of F1-F4, liquid chromatography is required for detecting the protein content of each band, and experiments on lodging resistance and disease resistance are required, and excellent single plant retention is required.
In a preferred embodiment of the present invention, the identification comprises yield, cold resistance, heat resistance, disease resistance, and grain firmness and maturity ear types.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, excellent high-yield individual plant selection is carried out on highland barley through radiation treatment, excellent high-temperature-resistant individual plants are selected through high-temperature stress treatment on wheat, two kinds of hybrid cultivation are carried out, backcross stable cultivation is carried out simultaneously, disease-resistant and cold-resistant detection is carried out for each generation, high-quality individual plants are selected for secondary cultivation, the protein content of the high-quality individual plants is detected, the individual plants with better quality are selected for cultivation, the green wheat variety with higher comprehensiveness is favorably cultivated, the cold resistance and the high-temperature resistance are combined, the planting latitude of the wheat is greatly increased, and meanwhile, the method can be suitable for planting on the land in the area with larger temperature difference.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
The invention provides a technical scheme that: a breeding method of a new green wheat variety, S1: selecting a highland barley variety as a male parent and a wheat variety as a female parent;
s2: placing highland barley in a vacuum closed environment, performing radiation treatment on the highland barley, placing the highland barley in a land for planting, and performing individual plant selection according to the yield;
s3: carrying out high-temperature stress on wheat seeds to plant wheat female parents, and carrying out single plant selection according to high-temperature resistance after 8-10 times of high-temperature stress treatment;
s4: carrying out hybridization cultivation on two single plants selected from the S2 and the S3, and selecting a high-yield single plant to form an F1 generation;
s5: hybridizing the excellent single plants of the F1 generation with the highland barley male parents again to finish the cultivation of the F2 generation;
s6: hybridizing the F2 generation excellent single plant with the wheat female parent again to finish F3 generation culture
S7: hybridizing the excellent F3 generation single plants with the F1 band again to finish F4 generation cultivation;
s8: planting F4 generations in multiple regions, and selecting high-yield high-temperature-resistant excellent single plants to form F5 generations;
s9: and planting the F5 generation for multiple generations, and identifying the F5 generation.
The method comprises the steps of treating soil in the early stage of wheat, carrying out deep tillage treatment in a mechanical mode, further enhancing the water and fertilizer retention effect of the soil, carrying out fine tillage and fine preparation on a seeding land block, carrying out tillage for 23-25cm, formulating a fertilization scheme according to the comprehensive fertility status of the soil, and applying organic fertilizer mainly and combining organic fertilizer and inorganic fertilizer;
after sowing, the pesticide is sprayed for 1-2 times in time to reduce the harm of virus diseases, so that the wheat seedlings are protected from growing, and the important function of topdressing is also emphasized to ensure sufficient fertility;
placing highland barley in a vacuum closed environment, carrying out non-ionizing radiation treatment on the highland barley for 30 seconds by using treatment power of 60W, placing the highland barley in a land for planting, and carrying out single plant selection according to conditions of high yield, large number of grains and high quality;
and (3) carrying out high-temperature stress on the wheat seeds to plant the wheat female parent, namely increasing the planting temperature to ensure that the wheat grows in a high-temperature environment, and selecting excellent single plants according to the single plants with the optimal leaf area ratio after 8-10 times of high-temperature stress treatment.
In the embodiment, the highland barley adopts Kunlun No. 10, and the wheat adopts Zhongmai 895.
In this embodiment, the highland barley seeds are treated with non-ionizing radiation at a treatment power of 60W for 30 seconds.
In the embodiment, the germination rate of the highland barley is recorded after the highland barley is planted, and the single plants with high yield, large number of grains and high quality are selected.
In this embodiment, after the wheat is stressed at a high temperature, a single plant with an optimal green leaf area ratio needs to be selected.
In this embodiment, the individual plants in S2 and S3 need to be processed at normal temperature, and then the protein content is detected by liquid chromatography, and the individual plants are preferably selected.
In the embodiment, in the S5, the hybridization adopts a emasculation pollination mode, the weak removing and strong retention are observed, the stem leaves are not damaged, and the bag is required to be sleeved and the label is attached after the pollination is finished.
In this example, in the cultivation of F1 to F4, liquid chromatography was required for each band to detect the protein content, and experiments for lodging resistance and disease resistance were required, and excellent individuals remained therein.
In this example, the identification content includes yield, cold resistance, heat resistance, disease resistance, grain firmness, and maturity ear types.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Cultivating highland barley and wheat in a laboratory, processing the soil structure in the laboratory to ensure the oxygen of the soil and increase the soil fertility, placing the highland barley in a vacuum closed environment, carrying out non-ionizing radiation treatment on the highland barley for 30 seconds by 60W, placing the highland barley in a land for planting, carrying out single plant selection according to the conditions of high yield, large number of grains and high quality, carrying out high-temperature stress on the wheat seeds to plant wheat female parents, namely increasing the planting temperature to ensure that the wheat grows in the high-temperature environment, selecting excellent single plants according to the single plant with the optimal leaf area after 8-10 of the high-temperature stress treatment, carrying out hybrid cultivation on the two excellent single plants, carrying out left-hand holding on the wheat ears once the wheat ears are selected, carrying out right-hand holding on tweezers to remove the wheat ears at the upper part and the base part of the wheat ears firstly, and keeping the wheat ears in the middle part of the wheat ears, then only two florets at the base of each spikelet are reserved in the middle part of each spikelet, the other florets are removed in a unified way, attention needs to be paid, the lowest florets of the spikelets are usually unnoticed and easy to be missed and forgotten to be removed, only ten well-developed and strongly-grown florets are left on the hybrid spikes through the spikelet trimming process, the spikelet with the whole spike is held by the right hand, the upper third of the husk of the wheat is cut off by the left hand with scissors, anthers in the flowers are removed, pollination is carried out 1-3 days after castration, mature pollen in a paternal garden is collected, the mature pollen of the paternal parent is sequentially placed into each castrated flower by a pollinator when the blossom is more abundant, a paper bag is favorable after pollination is finished, a high-yield single plant in the paper is selected to form an F1 generation, the excellent single plant of the F1 generation is hybridized with the highland barley male parent again, and F2 generation cultivation is finished, and (2) hybridizing the excellent F2 generation single plants with the wheat female parent again to complete F3 generation cultivation, hybridizing the excellent F3 generation single plants with the F1 generation again to complete F4 generation cultivation, planting F4 generation in multiple areas, selecting high-yield and high-temperature-resistant excellent single plants to form F5 generation, planting the F5 generation for multiple generations and identifying the F5 generation, wherein in the F1-F4 cultivation process, liquid chromatography is required to detect protein content in each band, lodging resistance and disease resistance experiments are required to be carried out, the excellent single plants are retained, and grain solidity and mature-period scion types are recorded.
In the cultivation process of F1-F4, need cultivate the planting in the area of high latitude, the great region of difference in temperature plants, and select wherein excellent individual plant, and the soil in area carries out the soil preparation, it needs to satisfy ploughing deeply and turns over deeply, deepen the plough layer, plough thoroughly and harrow thoroughly, no leaking and plowing is neglected, the soil is finely divided, no dark and strong soil, the ground is level, it is virtuality and reality, retaining soil is enough with the wall, and in the high latitude area northern area, most area is taken the maize straw field returning mode to carry out the soil preparation, need adopt returning field and ploughing deeply and combining together, make kibbling straw and turning into deep soil, do benefit to microbial decomposition, it is better to insist to smash more thin more, through rubbing formula machinery, length control avoids suddenly lacking air, run out the problem such as not good, after maize straw, soil microorganism need consume nitrogen element straw in soil in the decomposition process, according to replenish 10 kilograms of ammonium per one hundred per kilogram better, the straw is more than the better, the more the number of carbon is needed to be returned to control, the field returning to the field again to the disease and insect pest is not to the problem of returning to the field again to the soil again in order to avoid the soil.
When the breeding method of the new green wheat variety is used, it is to be noted that the breeding method of the new green wheat variety is realized in a universal standard component or a method known by a person skilled in the art, and the principle of the breeding method is known by the person skilled in the art through a technical manual or a conventional experimental method.
While there have been shown and described what are at present considered the fundamental principles and essential features of the invention and its advantages, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present specification describes embodiments, not every embodiment includes only a single embodiment, and such description is for clarity purposes only, and it is to be understood that all embodiments may be combined as appropriate by one of ordinary skill in the art to form other embodiments as will be apparent to those of skill in the art from the description herein.
Claims (9)
1. A breeding method of a new green wheat variety is characterized by comprising the following steps:
s1: selecting highland barley as male parent and wheat as female parent;
s2: placing highland barley in a vacuum closed environment, performing radiation treatment on the highland barley, placing the highland barley in a land for planting, and performing individual plant selection according to the yield;
s3: carrying out high-temperature stress on wheat seeds to plant wheat female parents, and carrying out single plant selection according to high-temperature resistance after 8-10 times of high-temperature stress treatment;
s4: carrying out hybridization cultivation on two single plants selected from the S2 and the S3, and selecting a high-yield single plant to form an F1 generation;
s5: hybridizing the excellent single plants of the F1 generation with the highland barley male parents again to finish the cultivation of the F2 generation;
s6: hybridizing the F2 generation excellent single plant with the wheat female parent again to finish F3 generation culture
S7: hybridizing the excellent F3 generation single plants with the F1 band again to finish F4 generation cultivation;
s8: planting F4 generations in multiple regions, and selecting high-yield high-temperature-resistant excellent single plants to form F5 generations;
s9: and planting the F5 generation for multiple generations, and identifying the F5 generation.
2. The breeding method of a new green wheat variety according to claim 1, characterized in that: the highland barley adopts Kunlun No. 10, and the wheat adopts Chinese barley 895.
3. The breeding method of a new green wheat variety according to claim 1, characterized in that: the highland barley seeds are treated by non-ionizing radiation with 60W of treatment power for 30 seconds.
4. The breeding method of a new green wheat variety according to claim 3, characterized in that: and recording the germination rate of the highland barley after planting, and selecting single plants with high yield, large number of grains and high quality.
5. The breeding method of a new green wheat variety according to claim 1, characterized in that: after the wheat is stressed at high temperature, a single plant with the optimal green leaf area ratio needs to be selected.
6. The breeding method of a new green wheat variety according to claim 1, characterized in that: and (4) treating the individual plants in the S2 and the S3 at normal temperature, detecting the protein content by liquid chromatography, and preferably selecting the individual plants.
7. The breeding method of a new green wheat variety according to claim 1, characterized in that: in S5, hybridization adopts a castration and pollination mode, the weak removal and strong retention are observed, the stem leaves are not damaged, bagging is needed after pollination is finished, and a label is attached.
8. The breeding method of a new green wheat variety according to claim 1, characterized in that: in the cultivation process of F1-F4, liquid chromatography is needed to detect the protein content of each band, and lodging resistance and disease resistance experiments are needed and excellent single plants are remained in the bands.
9. The breeding method of a new green wheat variety according to claim 1, characterized in that: the findings included yield, cold resistance, heat resistance, disease resistance, and grain firmness and maturity ear types.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211404499.3A CN115669533A (en) | 2022-11-10 | 2022-11-10 | Breeding method for new green wheat variety |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211404499.3A CN115669533A (en) | 2022-11-10 | 2022-11-10 | Breeding method for new green wheat variety |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115669533A true CN115669533A (en) | 2023-02-03 |
Family
ID=85052465
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211404499.3A Pending CN115669533A (en) | 2022-11-10 | 2022-11-10 | Breeding method for new green wheat variety |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115669533A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3056080A1 (en) * | 2015-02-10 | 2016-08-17 | Vyzkumne centrum SELTON, s.r.o. | Method of producing hybrid F1 seed of angiosperm plants |
| CN109076950A (en) * | 2018-08-09 | 2018-12-25 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | A kind of selection of two ribs naked barley |
| CN111602593A (en) * | 2020-06-12 | 2020-09-01 | 曲靖市农业科学院(曲靖市农业技术推广中心) | Male shearing hybridization method for wheat |
| CN111742836A (en) * | 2020-07-14 | 2020-10-09 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | Breeding method of hooked mango barley |
| CN114451296A (en) * | 2022-03-01 | 2022-05-10 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | Breeding method of black-grain double-edge highland barley |
-
2022
- 2022-11-10 CN CN202211404499.3A patent/CN115669533A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3056080A1 (en) * | 2015-02-10 | 2016-08-17 | Vyzkumne centrum SELTON, s.r.o. | Method of producing hybrid F1 seed of angiosperm plants |
| CN109076950A (en) * | 2018-08-09 | 2018-12-25 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | A kind of selection of two ribs naked barley |
| CN111602593A (en) * | 2020-06-12 | 2020-09-01 | 曲靖市农业科学院(曲靖市农业技术推广中心) | Male shearing hybridization method for wheat |
| CN111742836A (en) * | 2020-07-14 | 2020-10-09 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | Breeding method of hooked mango barley |
| CN114451296A (en) * | 2022-03-01 | 2022-05-10 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | Breeding method of black-grain double-edge highland barley |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107409662B (en) | Crop rotation method for reducing incidence of tobacco bacterial wilt | |
| CN104542256A (en) | Breeding method for importing false smut resistance of indica rice into japonica rice | |
| CN106962195A (en) | A kind of wild cabbage seeding technique | |
| CN108633669A (en) | Method for cultivating medium-ripened trailing short cowpea variety in south China in open field in summer | |
| CN104170724A (en) | Breeding method for aromatic hybrid rice Guangxiangyou 36 | |
| Bozoglu et al. | Determination of the yield performance and harvesting periods of fifteen pea (Pisum sativum L.) cultivars sown in autumn and spring | |
| CN108782091A (en) | Method for improving seed reproduction yield of asparagus bean in south China | |
| CN101263783B (en) | Method for using herbicide barban to fast breed aloe all-male plant strain | |
| CN106416995A (en) | Method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines | |
| CN104719125A (en) | Heat-resistant circular spherical white radish breeding selection method | |
| CN105766624B (en) | A kind of method of wart handle konjaku crossbreeding | |
| CN109329043B (en) | Breeding method of small-seed type pumpkin rootstock variety | |
| CN111657137A (en) | Oat hybridization method | |
| CN113455380B (en) | Cultivation method of salt-tolerant high-oil high-oleic acid peanut variety | |
| CN115777526A (en) | Breeding method of fine grain fragrance type high-quality temperature-sensitive rice genic male sterile line | |
| CN103609430B (en) | Method for selecting and reproducing early-flowering late japonica rice sterile line Zhe japonica rice 4A with high stigma exposure rate | |
| CN115669533A (en) | Breeding method for new green wheat variety | |
| CN113796307A (en) | Sunflower hybrid pollination breeding method under southern damp-heat condition | |
| CN101999310A (en) | Breeding and reproducing method of indica rice male sterile line Zhenxian 1A with high quality, rice blast resistance and high combining ability | |
| CN115836643B (en) | Method for improving distant hybridization rate of capsicum by pollen Mongolian means | |
| CN103718950A (en) | Method for breeding first generation of hybrid of tillered-onion | |
| CN114568301B (en) | Breeding method of high-quality and high-yield beer barley | |
| CN116076356B (en) | Method for breeding northern early-maturing short-stalk soybeans | |
| CN113906962B (en) | Crop seed quality improvement method based on quantum technology | |
| Dhiman et al. | No. 18 (IC635707; INGR20065), a Lily (Lilium sp.) Germplasm with Multiple Shoots/Sprouting, 100% Flowering and Larger Bud Size. Low Juvenile Period. No Vernalization Requirement |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |