CN115595328B - 天冬氨酸脱羧酶在发酵生产维生素b5中的应用 - Google Patents

天冬氨酸脱羧酶在发酵生产维生素b5中的应用

Info

Publication number
CN115595328B
CN115595328B CN202210214711.3A CN202210214711A CN115595328B CN 115595328 B CN115595328 B CN 115595328B CN 202210214711 A CN202210214711 A CN 202210214711A CN 115595328 B CN115595328 B CN 115595328B
Authority
CN
China
Prior art keywords
gene
artificial sequence
vitamin
coli
pand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202210214711.3A
Other languages
English (en)
Chinese (zh)
Other versions
CN115595328A (zh
Inventor
温廷益
刘树文
李忠财
孙佳慧
邓爱华
张芸
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Microbiology of CAS
Original Assignee
Institute of Microbiology of CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Microbiology of CAS filed Critical Institute of Microbiology of CAS
Priority to CN202210214711.3A priority Critical patent/CN115595328B/zh
Publication of CN115595328A publication Critical patent/CN115595328A/zh
Priority to EP23765746.5A priority patent/EP4458972A1/en
Priority to JP2024553525A priority patent/JP2025516090A/ja
Priority to US18/841,587 priority patent/US20260002145A1/en
Priority to PCT/CN2023/076309 priority patent/WO2023169168A1/zh
Application granted granted Critical
Publication of CN115595328B publication Critical patent/CN115595328B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01011—Aspartate 1-decarboxylase (4.1.1.11)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202210214711.3A 2022-03-07 2022-03-07 天冬氨酸脱羧酶在发酵生产维生素b5中的应用 Active CN115595328B (zh)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN202210214711.3A CN115595328B (zh) 2022-03-07 2022-03-07 天冬氨酸脱羧酶在发酵生产维生素b5中的应用
EP23765746.5A EP4458972A1 (en) 2022-03-07 2023-02-16 Use of aspartate decarboxylase in fermentation production of vitamin b5
JP2024553525A JP2025516090A (ja) 2022-03-07 2023-02-16 発酵によるビタミンｂ５の生産におけるアスパラギン酸デカルボキシラーゼの使用
US18/841,587 US20260002145A1 (en) 2022-03-07 2023-02-16 Application of aspartate decarboxylase in the production of vitamin b5 by fermentation
PCT/CN2023/076309 WO2023169168A1 (zh) 2022-03-07 2023-02-16 天冬氨酸脱羧酶在发酵生产维生素b5中的应用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210214711.3A CN115595328B (zh) 2022-03-07 2022-03-07 天冬氨酸脱羧酶在发酵生产维生素b5中的应用

Publications (2)

Publication Number Publication Date
CN115595328A CN115595328A (zh) 2023-01-13
CN115595328B true CN115595328B (zh) 2026-02-13

Family

ID=84842029

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210214711.3A Active CN115595328B (zh) 2022-03-07 2022-03-07 天冬氨酸脱羧酶在发酵生产维生素b5中的应用

Country Status (5)

Country Link
US (1) US20260002145A1 (https=)
EP (1) EP4458972A1 (https=)
JP (1) JP2025516090A (https=)
CN (1) CN115595328B (https=)
WO (1) WO2023169168A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115595328B (zh) * 2022-03-07 2026-02-13 中国科学院微生物研究所 天冬氨酸脱羧酶在发酵生产维生素b5中的应用
CN115595314B (zh) * 2022-03-07 2025-06-24 中国科学院微生物研究所 表达天冬氨酸脱氢酶的工程菌及发酵生产维生素b5的方法
CN119220576B (zh) * 2024-12-03 2025-07-25 浙江工业大学 基于一碳单位供应的产d-泛酸基因工程菌及构建与应用
CN121160743B (zh) * 2025-11-20 2026-03-13 中国科学院微生物研究所 一种ompR基因突变体及其在发酵生产VB5中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107828671A (zh) * 2010-11-22 2018-03-23 卡吉尔公司 用于生产3‑羟基丙酸的组合物和方法
CN109913398A (zh) * 2019-03-14 2019-06-21 浙江工业大学 无需β-丙氨酸添加的高产泛酸的基因工程菌、构建及应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2163629B1 (en) * 1999-09-21 2017-03-08 Basf Se Methods and microorganisms for production of panto-compounds
DE10132178A1 (de) * 2001-07-03 2003-01-23 Degussa Verfahren zur fermantativen Herstellung von D-Pantothensäure und/oder deren Salzen
CA2491145A1 (en) * 2002-07-03 2004-01-15 Basf Aktiengesellschaft Microorganisms and processes for enhanced production of pantothenate
JP2005531326A (ja) * 2002-07-03 2005-10-20 ビーエーエスエフ アクチェンゲゼルシャフト パントテン酸の高生産のための微生物及び方法
CN115595328B (zh) * 2022-03-07 2026-02-13 中国科学院微生物研究所 天冬氨酸脱羧酶在发酵生产维生素b5中的应用
CN115595314B (zh) * 2022-03-07 2025-06-24 中国科学院微生物研究所 表达天冬氨酸脱氢酶的工程菌及发酵生产维生素b5的方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107828671A (zh) * 2010-11-22 2018-03-23 卡吉尔公司 用于生产3‑羟基丙酸的组合物和方法
CN109913398A (zh) * 2019-03-14 2019-06-21 浙江工业大学 无需β-丙氨酸添加的高产泛酸的基因工程菌、构建及应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"Metabolic engineering of Escherichia coli for D-pantothenic acid production";Bo Zhang et al.;《Food Chemistry》;20190508;第294卷;第267–275页 *

Also Published As

Publication number Publication date
WO2023169168A1 (zh) 2023-09-14
US20260002145A1 (en) 2026-01-01
EP4458972A1 (en) 2024-11-06
CN115595328A (zh) 2023-01-13
JP2025516090A (ja) 2025-05-27

Similar Documents

Publication Publication Date Title
CN115595328B (zh) 天冬氨酸脱羧酶在发酵生产维生素b5中的应用
CN109750069B (zh) 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法
CN104498519A (zh) 一种表达重组载体及其应用
CN115595314B (zh) 表达天冬氨酸脱氢酶的工程菌及发酵生产维生素b5的方法
Zhang et al. Strengthening the (R)-pantoate pathway to produce D-pantothenic acid based on systematic metabolic analysis
CN102884179A (zh) 用于制备l-高丙氨酸的组合物和方法
CN106148259B (zh) 生产l-2-氨基丁酸的重组菌及其制备方法与应用
Wang et al. Coproduction of succinic acid and cadaverine using lysine as a neutralizer and CO 2 donor with L-lysine decarboxylase overexpressed Escherichia coli AFP111
CN108913724B (zh) 一种以丙二酸盐为原料合成3-羟基丙酸的制备方法及其相应重组细胞和应用
EP4538375A1 (en) Ep6 promoter, related biomaterial, and use thereof
CN119220576B (zh) 基于一碳单位供应的产d-泛酸基因工程菌及构建与应用
CN119220512B (zh) 酮醇酸还原异构酶突变体、高产d-泛酸的基因工程菌
CN119120516A (zh) 发酵生产维生素b5的工程菌及应用
CN117946984B (zh) 泛酸合成酶突变体及其制备方法、构建体及其构建方法、泛酸生产菌株和应用及泛酸的制备方法
CN121160743B (zh) 一种ompR基因突变体及其在发酵生产VB5中的应用
CN121203998A (zh) 一种提高β-烟酰胺单核苷酸产量的突变体组合，其编码基因、氨基酸序列及其应用
CN119120333B (zh) 一种重组微生物及其在生产l-异亮氨酸中的应用
CN119752907B (zh) 启动子突变体及其应用
CN116024278B (zh) 一种发酵法制备d-泛酸的方法
CN119391666B (zh) 一种异丙基苹果酸合酶突变体及其应用
CN121628937A (zh) 一种高产d-泛酸的基因工程菌及其构建方法、应用
CN121022701A (zh) 一种高产l-高丝氨酸的基因工程菌及其构建方法和应用
CN121592676A (zh) 一种乙醛脱氢酶及其编码基因与应用
CN120536327A (zh) 一种基于底物通道策略提升重组大肠杆菌l-组氨酸生产效率的方法及应用
CN111440752A (zh) 一种葡萄糖代谢缺陷型菌株的构建方法及其混菌发酵产1，5-戊二胺

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant