JP2025516090A - 発酵によるビタミンｂ５の生産におけるアスパラギン酸デカルボキシラーゼの使用 - Google Patents

発酵によるビタミンｂ５の生産におけるアスパラギン酸デカルボキシラーゼの使用 Download PDF

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JP2025516090A
JP2025516090A JP2024553525A JP2024553525A JP2025516090A JP 2025516090 A JP2025516090 A JP 2025516090A JP 2024553525 A JP2024553525 A JP 2024553525A JP 2024553525 A JP2024553525 A JP 2024553525A JP 2025516090 A JP2025516090 A JP 2025516090A
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coli
gene
vitamin
production
aspartate
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JP2025516090A5 (https=
Inventor
廷益 温
▲樹▼文 ▲劉▼
忠▲財▼ 李
佳慧 ▲孫▼
▲愛▼▲華▼ デン
芸 ▲張▼
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Institute of Microbiology of CAS
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Institute of Microbiology of CAS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01011—Aspartate 1-decarboxylase (4.1.1.11)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2024553525A 2022-03-07 2023-02-16 発酵によるビタミンｂ５の生産におけるアスパラギン酸デカルボキシラーゼの使用 Pending JP2025516090A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN202210214711.3A CN115595328B (zh) 2022-03-07 2022-03-07 天冬氨酸脱羧酶在发酵生产维生素b5中的应用
CN202210214711.3 2022-03-07
PCT/CN2023/076309 WO2023169168A1 (zh) 2022-03-07 2023-02-16 天冬氨酸脱羧酶在发酵生产维生素b5中的应用

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JP2025516090A true JP2025516090A (ja) 2025-05-27
JP2025516090A5 JP2025516090A5 (https=) 2025-12-12

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US (1) US20260002145A1 (https=)
EP (1) EP4458972A1 (https=)
JP (1) JP2025516090A (https=)
CN (1) CN115595328B (https=)
WO (1) WO2023169168A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115595328B (zh) * 2022-03-07 2026-02-13 中国科学院微生物研究所 天冬氨酸脱羧酶在发酵生产维生素b5中的应用
CN115595314B (zh) * 2022-03-07 2025-06-24 中国科学院微生物研究所 表达天冬氨酸脱氢酶的工程菌及发酵生产维生素b5的方法
CN119220576B (zh) * 2024-12-03 2025-07-25 浙江工业大学 基于一碳单位供应的产d-泛酸基因工程菌及构建与应用
CN121160743B (zh) * 2025-11-20 2026-03-13 中国科学院微生物研究所 一种ompR基因突变体及其在发酵生产VB5中的应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
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EP2163629B1 (en) * 1999-09-21 2017-03-08 Basf Se Methods and microorganisms for production of panto-compounds
DE10132178A1 (de) * 2001-07-03 2003-01-23 Degussa Verfahren zur fermantativen Herstellung von D-Pantothensäure und/oder deren Salzen
CA2491145A1 (en) * 2002-07-03 2004-01-15 Basf Aktiengesellschaft Microorganisms and processes for enhanced production of pantothenate
JP2005531326A (ja) * 2002-07-03 2005-10-20 ビーエーエスエフ アクチェンゲゼルシャフト パントテン酸の高生産のための微生物及び方法
US9090918B2 (en) * 2010-11-22 2015-07-28 Novozymes A/A Compositions and methods for 3-hydroxypropionic acid production
CN109913398B (zh) * 2019-03-14 2020-07-31 浙江工业大学 无需β-丙氨酸添加的高产泛酸的基因工程菌、构建及应用
CN115595328B (zh) * 2022-03-07 2026-02-13 中国科学院微生物研究所 天冬氨酸脱羧酶在发酵生产维生素b5中的应用
CN115595314B (zh) * 2022-03-07 2025-06-24 中国科学院微生物研究所 表达天冬氨酸脱氢酶的工程菌及发酵生产维生素b5的方法

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CN115595328B (zh) 2026-02-13
WO2023169168A1 (zh) 2023-09-14
US20260002145A1 (en) 2026-01-01
EP4458972A1 (en) 2024-11-06
CN115595328A (zh) 2023-01-13

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