CN115558036A - Preparation method of centella asiatica polysaccharide and application of centella asiatica polysaccharide in cosmetics - Google Patents
Preparation method of centella asiatica polysaccharide and application of centella asiatica polysaccharide in cosmetics Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of centella asiatica polysaccharide and application of the centella asiatica polysaccharide in cosmetics. Step 1, taking a herba Centellae medicinal material, refluxing for 2 times by using ethanol, adding 8 times of 80% ethanol each time for 2 hours, removing filtrate, and drying herb residues in the air; decocting the dried residues in water for 2 times, adding 10 times of water each time for 2 hr, filtering, and mixing filtrates; step 2, concentrating the water extract under reduced pressure until the concentration of crude drugs is 1-1.3 g/mL, slowly adding ethanol while stirring until the alcohol content reaches 70-80%, uniformly stirring, standing for 6-10 h, centrifuging for 10-15 min, and recovering supernatant under reduced pressure; 3, dissolving the precipitate with water, and repeating the alcohol precipitation step; and step 4, combining the obtained product, precipitating the obtained product by alcohol precipitation for 2 times, drying the obtained product at 70-75 ℃, and crushing and sieving the dried product to obtain the asiatic pennywort herb polysaccharide. The method has the advantages of simple process, easy operation, low cost and easy popularization; has remarkable antioxidant activity, and can be used as natural antioxidant in cosmetics with antioxidant effect.
Description
Technical Field
The invention belongs to the field of cosmetics, and particularly relates to a preparation method of centella asiatica polysaccharide and application of the centella asiatica polysaccharide in cosmetics.
Background
Plant polysaccharides extracted from plant raw materials are important components of polysaccharide families, and the plant polysaccharides have various biological activities due to the specificity and complexity of conformation, so that the development, utilization and research of polysaccharide resources are more and more concerned, and the plant polysaccharides become one of the research hotspots in recent years and are widely applied to a plurality of fields such as food, daily chemicals, medicines and the like. Along with the demand of people on beautiful life, people pay more and more attention to pursuing healthy functional cosmetics, and more favor plant natural product cosmetics with natural sources and no toxic or side effect so as to achieve the aims of protecting and beautifying skin. Compared with the original cosmetics or cosmetics containing chemical components, the plant polysaccharide shows a plurality of natural skin care physiological activities on one hand, and has various natural pharmacological effects of moisturizing, resisting wrinkles, removing acnes, resisting oxidation, whitening, resisting bacteria, resisting allergy, diminishing inflammation, removing mites, healing wounds and the like, and is safe to use and mild in effect; on the other hand, due to the existence of hydrophilic groups, the plant polysaccharide has strong hydrophilicity, gel property, emulsifying property and film forming property and low exclusivity, can ensure high affinity with human skin when being added into daily cosmetics, and has wider development and application space compared with chemical synthesis cosmetics on the market.
Centella asiatica (L.) urban is a dry whole plant of Centella asiatica of Umbelliferae, and has long application history and abundant resources. Modern medical research shows that centella asiatica has physiological effects of resisting depression, promoting wound healing, treating scars, resisting tumors, gastric ulcer, bacteria, oxidation and the like. At present, the research on centella asiatica is mainly focused on its saponins and sapogenins, including asiaticoside, madecassoside, asiatic acid and madecassic acid. Wherein, asiaticoside and madecassoside are main effective components in herba Centellae, and have effects of promoting wound healing, repairing skin scar, resisting allergy, resisting inflammation, whitening, resisting oxidation, resisting gastric ulcer and treating hepatitis. There are also a number of cosmetic products on the market which have centella asiatica extract or saponin monomer components thereof as active additives. The research on centella asiatica polysaccharide is relatively few, and the patent CN110317279B discloses the centella asiatica polysaccharide, the application of the centella asiatica polysaccharide as a plant source preservative and the application of the centella asiatica polysaccharide in cosmetics, and only discloses the bacteriostatic activity of the centella asiatica polysaccharide; patent CN112656707A discloses the use of centella asiatica polysaccharide extract in anti-inflammatory aspect, disclosing that centella asiatica polysaccharide extract has anti-inflammatory activity in vivo. At present, the efficacy of centella asiatica polysaccharide in other aspects is not reported.
Disclosure of Invention
The invention aims to provide centella asiatica polysaccharide extracted and prepared from centella asiatica, which has good antioxidant activity and can be applied to related cosmetics.
The invention also aims to provide a preparation method of the asiatic pennywort herb polysaccharide.
The purpose of the invention is realized by the following technical scheme:
the invention provides a preparation method of centella asiatica polysaccharide, which comprises the following steps:
taking a herba Centellae medicinal material, refluxing with ethanol for 2 times, adding 8 times of 80% ethanol each time for 2 hours, removing the filtrate, and drying the residue. Decocting the residue in water for 2 times, each time adding 10 times of water for 2 hr, filtering, and mixing filtrates.
And (2) concentrating the water extract under reduced pressure until the concentration of the crude drug is 1-1.3 g/mL, slowly adding ethanol while stirring until the alcohol content reaches 70-80%, uniformly stirring, standing for 6-10 h, centrifuging for 10-15 min (4000-4500 r/min), and recovering the supernatant under reduced pressure.
And (4) dissolving the precipitate with water, and repeating the alcohol precipitation step in the step (2). Dissolving the precipitate with water, slowly adding ethanol while stirring again until the ethanol content reaches 70-80%, stirring uniformly, standing for 6-10 h, and centrifuging for 10-15 min (4000-4500 r/min).
And (4) combining the precipitates after 2 times of alcohol precipitation, drying at 70-75 ℃, crushing and sieving to obtain the asiatic pennywort herb polysaccharide.
Wherein, the number of the sieving meshes in the step (4) is 55-65 meshes.
The asiatic pennywort herb polysaccharide prepared by the method has good antioxidation as shown by an activity test result, and can be used as an antioxidant for preparing cosmetics.
The invention further provides an application of the asiatic pennywort herb polysaccharide in cosmetics, and the cosmetics comprise the asiatic pennywort herb polysaccharide extract obtained by adopting the method and auxiliary materials.
Wherein the concentration of centella asiatica polysaccharide in the cosmetic is 4mg/ml.
Wherein said centella asiatica polysaccharide is used as an antioxidant in cosmetics.
The invention relates to a preparation method of centella asiatica polysaccharide and application thereof in cosmetics, which has the advantages that:
(1) The preparation method of the centella asiatica polysaccharide has the advantages of simple process, easiness in operation, low cost, easiness in popularization and application and the like, ethanol is not added in the extraction process, other chemical reagents are not needed, and the preparation process is green and environment-friendly. (2) The centella asiatica polysaccharide has remarkable antioxidant activity, can remove DPPH free radicals by 75.36 percent, can remove ABTS free radicals by 99.48 percent, has total antioxidant capacity by 1.01 percent, and can be used as a natural antioxidant to be applied to cosmetics with antioxidant effect.
Drawings
FIG. 1 shows the DPPH radical scavenging ability of centella asiatica polysaccharides of different mass concentrations
FIG. 2 scavenging ability of centella asiatica polysaccharides on ABTS free radicals at different mass concentrations
FIG. 3 Total antioxidant capacity of centella asiatica polysaccharides with different mass concentrations
Detailed Description
The following examples are intended to illustrate the essence of the present invention, but should not be construed as limiting the scope of the present invention.
Example 1: preparation of centella asiatica polysaccharide
Reflux-extracting herba Centellae with 80% ethanol for 2 times, adding 8 times of 80% ethanol each time, extracting for 2 hr each time, discarding filtrate, and air-drying the residue. Decocting the dried residue in water for 2 times, adding 10 times of water each time, extracting for 2 hr each time, filtering, and mixing filtrates. Concentrating the water extractive solution under reduced pressure to crude drug concentration of 1.2g/mL, slowly adding ethanol under stirring until ethanol content reaches 75%, stirring, standing for 10 hr, centrifuging for 15min (4500 r/min), recovering supernatant under reduced pressure, dissolving precipitate with water, precipitating with ethanol by the same method, and repeating for 2 times. Mixing the precipitates after 2 times of alcohol precipitation, oven drying at 75 deg.C, pulverizing, and sieving to obtain herba Centellae polysaccharide.
Example 2:
reflux-extracting herba Centellae with 80% ethanol for 2 times, adding 8 times of 80% ethanol each time, extracting for 2 hr each time, discarding filtrate, and air-drying the residue. Decocting the dried residue in water for 2 times, adding 10 times of water each time, extracting for 2 hr each time, filtering, and mixing filtrates. Concentrating the water extractive solution under reduced pressure to crude drug concentration of 1g/mL, slowly adding ethanol under stirring until ethanol content reaches 70%, stirring, standing for 6 hr, centrifuging for 10min (4000 r/min), recovering supernatant under reduced pressure, dissolving precipitate with water, precipitating with ethanol by the same method, and repeating for 2 times. Mixing the precipitates after 2 times of alcohol precipitation, oven drying at 70 deg.C, pulverizing, and sieving to obtain herba Centellae polysaccharide.
Example 3:
reflux-extracting herba Centellae with 80% ethanol for 2 times, adding 8 times of 80% ethanol each time, extracting for 2 hr each time, discarding filtrate, and air-drying the residue. Decocting the dried residues in water for 2 times, adding 10 times of water each time, extracting for 2 hr each time, filtering, and mixing filtrates. Concentrating the water extract under reduced pressure to crude drug concentration of 1.3g/mL, slowly adding ethanol under stirring until ethanol content reaches 80%, stirring, standing for 8 hr, centrifuging for 13min (4300 r/min), recovering supernatant under reduced pressure, dissolving precipitate with water, precipitating with ethanol by the same method, and repeating for 2 times. Mixing the precipitates after 2 times of alcohol precipitation, oven drying at 73 deg.C, pulverizing, and sieving to obtain herba Centellae polysaccharide.
Example 4: reflux-extracting herba Centellae with 80% ethanol for 2 times, adding 8 times of 80% ethanol each time, extracting for 2 hr each time, discarding filtrate, and air-drying the residue. Decocting the dried residue in water for 2 times, adding 10 times of water each time, extracting for 2 hr each time, filtering, and mixing filtrates. Concentrating the water extractive solution under reduced pressure to crude drug concentration of 1.1g/mL, slowly adding ethanol under stirring until the alcohol content reaches 78%, stirring, standing for 10 hr, centrifuging for 12min (4100 r/min), recovering supernatant under reduced pressure, dissolving precipitate with water, precipitating with ethanol by the same method, and repeating for 2 times. Mixing the precipitate after 2 times of alcohol precipitation, oven drying at 74 deg.C, pulverizing, and sieving to obtain centella asiatica polysaccharide.
Example 5: reflux-extracting herba Centellae with 80% ethanol for 2 times, adding 8 times of 80% ethanol each time, extracting for 2 hr each time, discarding filtrate, and air-drying the residue. Decocting the dried residue in water for 2 times, adding 10 times of water each time, extracting for 2 hr each time, filtering, and mixing filtrates. Concentrating the water extractive solution under reduced pressure to crude drug concentration of 1.4g/mL, slowly adding ethanol under stirring until ethanol content reaches 72%, stirring, standing for 10 hr, centrifuging for 11min (4200 r/min), recovering supernatant under reduced pressure, dissolving precipitate with water, precipitating with ethanol by the same method, and repeating for 2 times. Mixing the precipitates after 2 times of alcohol precipitation, oven drying at 71 deg.C, pulverizing, and sieving to obtain herba Centellae polysaccharide.
Example 6: antioxidant activity assay of centella asiatica polysaccharides (DPPH scavenging experiment)
The radical scavenging activity of the centella asiatica polysaccharides obtained in example 1 was determined by DPPH method. The determination steps are as follows: samples of centella asiatica polysaccharide prepared in example 1 were prepared in 1, 2 and 4mg/ml solutions with deionized water. Precisely sucking 100 mu L of sample solution with each concentration and 100 mu L of 0.1mmol/L DPPH solution as experimental groups, replacing 100 mu L of absolute ethyl alcohol with 100 mu L of absolute ethyl alcohol in a control group, adding 100 mu L of DPPH solution and 100 mu L of absolute ethyl alcohol as standard groups, reacting for 30min in a dark place, and then measuring the absorbance at 517nm by using a microplate reader. The clearance was calculated from the following formula: DPPH clearance = [1- (experimental group-a control group)/standard group a ] × 100% (1), the results are shown in table 1 and fig. 1.
TABLE 1 measurement results of radical scavenging activity by DPPH method
Example 7: determination of antioxidant Activity of centella asiatica polysaccharide (ABTS method)
The antioxidant activity of the centella asiatica polysaccharide prepared in example 1 was measured by the ABTS method. The determination steps are as follows: the preparation method of the ABTS +. Stock solution is as follows: preparing 2.45mmol/L potassium persulfate solution, dissolving ABTS powder with potassium persulfate solution to obtain 7mmol/L ABTS +. Stock solution, and storing in a refrigerator at 4 deg.C in dark place for use. The preparation method of the ABTS +. Determination solution is as follows: ABTS +. Stock solution was diluted with 10mmol/L phosphate buffer (pH = 7.4) to an absorbance of 0.7 ± 0.02 (734 nm). The centella asiatica polysaccharide samples prepared in example 1 were prepared in solutions of 1, 2 and 4mg/ml with deionized water. 0.2mL of the sample was added to 2.8mL of the ABTS + measurement solution, mixed, left for 10min, and then the absorbance (A) was measured at 734nm i ). Each sample was run in parallel 3 times. Absorbance A of the measurement solution was measured by replacing ABTS + with distilled water j Measurement of light absorption by Using distilled Water instead of sample solutionDegree A 0 . Substituting the data into the following formula to calculate the ABTS free radical clearance rate of each sample: ABTS free radical clearance (%) = [1- (A) i -A j )/A 0 ]X 100, and the results are shown in table 2 and fig. 2.
TABLE 2ABTS method determination of antioxidant Activity
Example 8: antioxidant activity determination of centella asiatica polysaccharide (FRAP method for determining total antioxidant value)
The FRAP method was used to determine the total antioxidant value of the centella asiatica polysaccharides prepared in example 1. The determination steps are as follows: the preparation method of the tripyridyltriazine TPTZ working solution is as follows, wherein the tripyridyltriazine TPTZ working solution is prepared from 300mmol/L (pH = 3.6) of acetic acid buffer solution: 10mmol/L TPTZ solution: 20mmol/L FeCl 3 1, preparing the current formulation. The centella asiatica polysaccharide samples prepared in example 1 were prepared in solutions of 1, 2 and 4mg/ml with deionized water. And (3) taking 100 mu L of each sample solution by a pipette, adding 3mL of FRAP working solution, uniformly mixing, incubating at 37 ℃ for 30min, and reading the absorbance at 593 nm. With FeSO 4 Drawing a standard curve for standard substances, and preparing 1mmol/L FeSO 4 The solution (2) was diluted to a gradient solution having a concentration of 0.2, 0.4, 0.6, 0.8mmol/L, and a regression equation was obtained, and the antioxidant capacity of the sample was expressed by FRAP value: 1FRAP Unit =1mmol/FeSO 4 I.e. the sample has an oxidation resistance equivalent to that of FeSO 4 The mmol/L number of the obtained product is shown in Table 3 and FIG. 3.
TABLE 3 experiment results of the FRAP method for determining the total oxidation resistance value
As can be seen from the above examples, when the concentration of centella asiatica polysaccharide is 4mg/ml, the clearance rate of DPPH free radicals is 75.36%, the clearance rate of ABTS free radicals is 99.48%, and the total antioxidant capacity is 1.01, which shows that the centella asiatica polysaccharide has good antioxidant capacity, and therefore, the centella asiatica polysaccharide can be used as a natural antioxidant in cosmetics with antioxidant effect.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that the scope of the invention is not limited to this embodiment.
Claims (6)
1. A preparation method of centella asiatica polysaccharide is characterized by comprising the following steps: the method comprises the following steps:
step (1), taking a centella asiatica medicinal material, refluxing for 2 times by using ethanol, adding 8 times of 80% ethanol for each time, removing filtrate after each 2-hour treatment, and airing dregs of a decoction; decocting the dried residues in water for 2 times, adding 10 times of water each time for 2 hr, filtering, and mixing filtrates;
step (2), concentrating the water extract under reduced pressure until the concentration of crude drug is 1-1.3 g/mL, slowly adding ethanol while stirring until the alcohol content reaches 70-80%, uniformly stirring, standing for 6-10 h, centrifuging for 10-15 min, and recovering supernatant under reduced pressure;
dissolving the precipitate with water, and repeating the alcohol precipitation step in the step (2);
and (4) combining the precipitates after 2 times of alcohol precipitation, drying at 70-75 ℃, crushing and sieving to obtain the asiatic pennywort herb polysaccharide.
2. The method for preparing centella asiatica polysaccharide according to claim 1, wherein the method comprises the following steps: the speed of centrifugation in the step (2) and the step (3) is 4000-4500 r/min.
3. The method of claim 1, wherein the step of preparing the centella asiatica polysaccharide comprises: the number of the screening meshes in the step (4) is 55-65 meshes.
4. Use of centella asiatica polysaccharides in cosmetics, said cosmetics comprising the centella asiatica polysaccharides extract obtained by the method according to any one of claims 1 to 3 and adjuvants.
5. The use of centella asiatica polysaccharide according to claim 4 in cosmetics, wherein: the concentration of the centella asiatica polysaccharide in the cosmetic is 4mg/ml.
6. The use of centella asiatica polysaccharide according to claim 4 in cosmetics, wherein: the centella asiatica polysaccharide is used as an antioxidant in cosmetics.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109330931A (en) * | 2018-10-11 | 2019-02-15 | 浙江大学自贡创新中心 | A kind of Gotu Kola P.E and preparation method thereof applied to cosmetics |
| CN110317279A (en) * | 2019-08-19 | 2019-10-11 | 姜东生 | A kind of Asiatic polysaccharide, the purposes as plant source antiseptic agent and the application in cosmetics |
| CN112656707A (en) * | 2020-12-29 | 2021-04-16 | 中华全国供销合作总社南京野生植物综合利用研究所 | Extraction method of centella asiatica polysaccharide extract, centella asiatica polysaccharide extract and application thereof |
| CN113171321A (en) * | 2021-05-11 | 2021-07-27 | 清远市望莎生物科技有限公司 | Centella asiatica extract and application thereof in preparation of anti-inflammatory cosmetics |
| CN113456796A (en) * | 2020-03-31 | 2021-10-01 | 无限极（中国）有限公司 | Anti-aging composition and preparation method and application thereof |
-
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- 2022-10-23 CN CN202211298411.4A patent/CN115558036A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109330931A (en) * | 2018-10-11 | 2019-02-15 | 浙江大学自贡创新中心 | A kind of Gotu Kola P.E and preparation method thereof applied to cosmetics |
| CN110317279A (en) * | 2019-08-19 | 2019-10-11 | 姜东生 | A kind of Asiatic polysaccharide, the purposes as plant source antiseptic agent and the application in cosmetics |
| CN113456796A (en) * | 2020-03-31 | 2021-10-01 | 无限极（中国）有限公司 | Anti-aging composition and preparation method and application thereof |
| CN112656707A (en) * | 2020-12-29 | 2021-04-16 | 中华全国供销合作总社南京野生植物综合利用研究所 | Extraction method of centella asiatica polysaccharide extract, centella asiatica polysaccharide extract and application thereof |
| CN113171321A (en) * | 2021-05-11 | 2021-07-27 | 清远市望莎生物科技有限公司 | Centella asiatica extract and application thereof in preparation of anti-inflammatory cosmetics |
Non-Patent Citations (2)
| Title |
|---|
| 唐春红: "《天然防腐剂与抗氧化剂》", vol. 1, 中国轻工业出版社, pages: 182 * |
| 韦媛媛等: "积雪草多糖的脱脂工艺研究", 《广西科技大学学报》, vol. 25, no. 2, pages 90 - 94 * |
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