CN110613644A

CN110613644A - Skin care emulsion containing cannabidiol nanoemulsion and preparation method thereof

Info

Publication number: CN110613644A
Application number: CN201910895727.3A
Authority: CN
Inventors: 李映波; 冯红; 宋小雪; 孙笠雯; 罗凡雅
Original assignee: Yunnan Chenjinwei Biotechnology Co Ltd
Current assignee: Yunnan Chenjinwei Biotechnology Co Ltd
Priority date: 2019-09-21
Filing date: 2019-09-21
Publication date: 2019-12-27

Abstract

The invention provides a skin care emulsion containing cannabidiol nanoemulsion and a preparation method thereof, which are characterized by being prepared from the following components in percentage by mass: 10-50% of CBD nano-emulsion, 0.5-3% of seville orange flower extract, 0.5-3% of kapok extract, 0.3-5% of elsholtzia extract, 1-5% of hydrogenated lecithin, 1-10% of silicone, 1-5% of collagen, 1-8% of urea, 0.01-0.03% of potassium sorbate, 0.01-0.05% of guar gum, 0.1-0.5% of cholesterol, 0.5-3% of 1, 2-hexanediol and the balance of deionized water, wherein the total amount of the components is 100%. The nano-emulsion has the surface effect and small-size effect of the nano-emulsion, not only can the solubility be changed, but also the curative effect of the medicine can be improved, and particularly after the CBD nano-emulsion, the seville orange flower extract, the kapok extract and the elsholtzia extract are added, the nano-emulsion is cooperated with the effects of whitening the skin, resisting inflammation, resisting oxidation, eliminating free radicals, lightening spots, whitening, supplementing water, promoting the metabolism of the skin, supplementing nutrient components in time and preventing the skin from being damaged by the external environment.

Description

Skin care emulsion containing cannabidiol nanoemulsion and preparation method thereof

Technical Field

The invention relates to the field of cosmetics, and particularly relates to a skin care emulsion containing cannabidiol CBD nano-emulsion and a preparation method thereof.

Background

With the age, the probability of the skin color spots is obviously increased, which is mainly characterized in that the face is different from the surrounding skin, and the spot formation mainly comprises various uncertain factors such as heredity, ultraviolet irradiation, drug influence, pregnancy and the like. Although the pain can not be brought to people, the beauty is influenced to a certain extent. And with the increasingly fierce pursuit of beauty, more people put the center of gravity on the basic skin care, and in numerous cosmetics, the water emulsion is widely applied by more people due to the unique advantages of the water emulsion as the basic skin care product.

The emulsion is a liquid cream cosmetic, has good effects of moistening and moisturizing skin, whitening skin and resisting oxidation, has a large water content, can provide moisture for the skin, moisturize and lock water, improve dry and sensitive skin, provide skin nutrition, and make the skin soft, glossy and elastic. However, the existing emulsion contains a large amount of chemical components and hormones, has few natural components and poor skin care effect, and needs further improvement.

Cannabidiol CBD is a medicinal chemical component extracted from industrial cannabis. Research data show that the CBD has application value in the aspects of reducing inflammation, sterilizing, resisting anxiety, pain, epilepsy and the like. In addition, it has good effects of scavenging free radicals, fading color spots, repairing injury, resisting oxidation and resisting stimulation. However, in the cosmetic field, the water solubility is poor, so that the permeation rate is reduced, the skin absorption is incomplete, effective resources are wasted, and the like.

Chinese patent CN 107982080 a discloses the use of cannabidiol or cannabis sativa extract in the preparation of whitening products, suggesting that cannabidiol or cannabis sativa leaf extract can effectively inhibit the formation of melanin, and has whitening effect. Chinese patent CN 107811904A discloses an anti-inflammatory and freckle-removing vanishing cream and a preparation method thereof, the prepared vanishing cream is added with rich bisabolol and cannabidiol extracts, and has obvious anti-inflammatory and freckle-removing effects, wherein the bisabolol synergistically enhances the anti-inflammatory and freckle-removing effects of cannabidiol, and can maintain the cannabidiol to play stronger anti-inflammatory and freckle-removing effects in a longer time

Chinese patent CN 109223667A discloses an effective whitening skin care cream, which is mainly added with centella extract, fig extract, daisy extract, oldenlandia diffusa extract, sanguisorba extract and other extracts, so that the skin care cream can nourish the skin of a human body while decontaminating, and effectively avoids the phenomena of dry and itching skin, allergy and the like caused by long-term use of bath cream. Chinese patent CN 108553375A discloses a preparation method of whitening and spot-fading emulsion, and the prepared emulsion is mainly added with licorice extract, maca extract and buckwheat starch to prepare granular cold-water-soluble buckwheat starch, so that the whitening and spot-fading emulsion has excellent whitening and spot-fading and anti-aging effects and is rich in various moisturizing components and amino acids. However, the existing cannabidiol skin care emulsion directly adds the cannabidiol CBD extract into the formula, which causes low curative effect, large loss of active ingredients, low bioavailability and poor transdermal absorption effect. Other Chinese herbal medicine skin care lotions have poor percutaneous absorption effect and slow or poor medicinal curative effect, and can not solve the problems of poor skin state and the like.

Therefore, there is a need for improvements in the prior art.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a skin care emulsion containing cannabidiol CBD nanoemulsion and a preparation method thereof, wherein the skin care emulsion can be used for removing free radicals, fading color spots, repairing injuries, resisting oxidation and resisting stimulation.

The invention is realized by the following technical scheme: a skin care emulsion containing cannabidiol CBD nanoemulsion is characterized by being prepared from the following components in percentage by mass:

the sum of the above components is 100%.

The CBD nanoemulsion is prepared by the following method:

preparing materials according to the following mass ratio:

the sum of the components is 100 percent;

A. under the condition of keeping out of the sun, directly adding cannabidiol CBD into MCT oil and peppermint oil, heating at 30-50 ℃, stirring until the cannabidiol CBD is completely dissolved in the oil phase, and cooling to room temperature to obtain an oil solution;

B. adding deionized water into the mineral concentrated solution, and stirring to obtain water soluble substance;

C. heating EL-20, EL-40 and sorbitol solution at 30-50 ℃ for dissolving, then uniformly mixing with the oil soluble substance in the step A, adding the water soluble substance in the step B, stirring at the temperature of 30-50 ℃ and the rotation speed of 600r/min for 400-.

The cannabidiol CBD is a commercially available product extracted from industrial cannabis sativa with THC less than 0.3%, and has effects of whitening skin, anti-inflammatory, antioxidant, and scavenging free radicals.

The mineral concentrated solution is a commercial product, contains various minerals and trace elements required by a human body, is in an ionic state, has strong permeability, is easy to be absorbed by the human body, and can effectively promote the metabolism of skin, activate cells and relieve the skin.

The seville orange flower extract is obtained by the following method: pulverizing dried flower of Rutaceae and Citrus, extracting with ethanol by conventional cold soaking, warm soaking or heating reflux extraction, filtering, recovering ethanol from the filtrate under reduced pressure, and vacuum freeze drying or spray drying the residue to obtain seville orange flower extract; wherein the mass concentration of the ethanol is 75 to

90 percent, and the adding amount of the ethanol is 10 to 40 times of the mass of the dried pollen; the cold soaking is carried out for 1-10 days at room temperature, the warm soaking is carried out for 1-3 hours at the temperature of 40-70 ℃, and the extraction times are 1-3 times; the heating reflux extraction is carried out for 1-3 times at 40-70 ℃, and each time lasts for 30-120 minutes; pre-freezing for 5-10 hours at the condensation temperature of-20 to-50 ℃ and the vacuum degree of 5-20 KPa in vacuum freeze drying; the air inlet temperature of spray drying is 100-300 ℃, and the air outlet temperature is 30-150 ℃.

The seville orange flower has the effects of soothing liver and regulating stomach, promoting blood circulation, relieving tension, accelerating metabolism, regulating water and oil balance of skin, improving rough skin, reducing fat and losing weight and the like.

The kapok extract is extracted by the following operations: pulverizing flos Bombacis Malabarici, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Bombacis Malabarici extract; wherein the adding amount of the distilled water is 10-50 times of the mass of the ceiba pollen, the cold soaking is to soak for 1-10 days at room temperature, the warm soaking is to soak for 1-3 hours at 40-60 ℃, and the extraction times are 1-3 times; the ultrasonic-assisted extraction is carried out at the temperature of 40-60 ℃, the ultrasonic power of 100-650W, the ultrasonic frequency of 19-23 KHz and the ultrasonic output power of 70-100%, the extraction times are 1-3, and each time lasts for 30-60 minutes; pre-freezing for 3-16 hours at the condensation temperature of-20 to-50 ℃ in vacuum freeze drying, wherein the vacuum degree is 5-20 KPa; the air inlet temperature of spray drying is 100-300 ℃, and the air outlet temperature is 30-140 ℃.

The common bombax flower has the effects of relieving swelling, removing blood stasis, clearing away heat and toxic materials, promoting urination, promoting blood circulation and the like.

The elsholtzia extract is prepared by the following method: pulverizing dried herba Moslae, sieving with 20 mesh sieve, adding 75-90% ethanol at a material-liquid ratio of 1:8, ultrasonic extracting for 3 times, 1-3 h/time, filtering, mixing filtrates, mixing 3 times of filtrates, performing suction filtration overnight in ice bath to obtain extractive solution, recovering ethanol under reduced pressure, and concentrating on 40-50 deg.C water bath to obtain herba Moslae extract.

The herba Moslae has effects of relieving exterior syndrome, inducing perspiration, stimulating digestive gland and gastrointestinal peristalsis, eliminating dampness, regulating stomach, relieving summer-heat, promoting lung qi circulation, inducing diuresis, and relieving swelling.

The hydrogenated lecithin, the silicone, the urea, the collagen, the potassium sorbate, the guar gum, the cholesterol and the 1, 2-hexanediol are all commercially available cosmetic-grade products.

The skin care emulsion containing cannabidiol CBD nanoemulsion provided by the invention is prepared by the following steps:

firstly, fully mixing guar gum and deionized water, standing overnight in a refrigerator at 4 ℃ for full swelling to obtain swelling liquid, adding cholesterol and 1, 2-hexanediol, carrying out ice bath ultrasonic treatment at 4 ℃, wherein the ultrasonic power is 10-20%, the ultrasonic on time is 5-10s, the ultrasonic off time is 5-10s, the ultrasonic duration is 10-25min, recovering to room temperature after ultrasonic treatment is finished, adding potassium sorbate and CBD nano-emulsion, and stirring for 5-15min at the rotating speed of 400-600r/min at 40-50 ℃ to obtain a material A;

uniformly mixing collagen, urea, silicone, hydrogenated lecithin and a proper amount of deionized water, and stirring at the temperature of 50-75 ℃ and the rotating speed of 600r/min for 300-;

and thirdly, adding the material B in the second step into the material A in the first step, stirring and mixing uniformly, adding the CBD nanoemulsion, the seville orange flower extract, the common bombax flower extract and the Chinese mosla herb extract, and fully mixing uniformly to obtain the skin care emulsion containing the cannabidiol CBD nanoemulsion.

The invention has the following advantages and effects:

the invention has the surface effect and small-size effect of the nano-emulsion, can change the solubility and can increase the curative effect of the medicine. Particularly, after the CBD nanoemulsion, the seville orange flower extract, the kapok extract and the elsholtzia extract are added, the medicinal effects of the seville orange flower extract, the kapok extract and the elsholtzia extract are cooperated with the skin whitening, anti-inflammatory, antioxidant and free radical removing effects of the CBD nanoemulsion, so that the skin whitening, skin metabolism promotion and timely skin nutrient supplement are realized. The skin care product can keep the curative effect of the medicine to the maximum extent, whitens the skin while lightening spots, replenishes water, enables the skin to form an isolation layer, prevents the skin from being damaged by the external environment, and can promote the follow-up skin care product to be well fitted and absorbed.

Detailed description of the invention

The present invention will be further described with reference to the following examples.

Example 1

A skin care emulsion containing cannabidiol CBD nanoemulsion is prepared by the following steps:

(1) preparing materials according to the following mass ratio:

(1) fully mixing guar gum and deionized water, standing overnight in a refrigerator at 4 ℃ for full swelling to obtain a swelling liquid, adding cholesterol and 1, 2-hexanediol, carrying out ice bath ultrasonic treatment at 4 ℃, wherein the ultrasonic power is 10%, the ultrasonic on time is 5s, the ultrasonic off time is 5s, the ultrasonic duration is 15min, recovering to room temperature after finishing ultrasonic treatment, adding potassium sorbate and CBD nano-emulsion, and stirring at the constant temperature of 45 ℃ for 5min at the speed of 600r/min to obtain a material A;

(2) uniformly mixing collagen, urea, silicone, hydrogenated lecithin and a proper amount of deionized water, and stirring at 50 ℃ and a rotating speed of 500r/min until the collagen, the urea, the silicone, the hydrogenated lecithin and the deionized water are dissolved to obtain a material B;

(3) and (3) adding the material B in the step (2) into the material A in the step (1), uniformly stirring, adding the CBD nanoemulsion, the seville orange flower extract, the kapok extract and the elsholtzia extract, and fully and uniformly mixing to obtain the skin care emulsion containing the cannabidiol CBD nanoemulsion.

The CBD nanoemulsion is prepared by the following method:

preparing materials according to the following mass ratio:

a, under the condition of keeping out of the sun, directly adding cannabidiol CBD into MCT oil and peppermint oil, heating in a water bath at 35 ℃, stirring until the cannabidiol CBD is completely dissolved in an oil phase, and cooling to room temperature to obtain an oil solution;

b, adding deionized water into the mineral concentrated solution, and uniformly stirring to obtain a water soluble substance;

and C, heating and dissolving the EL-20, EL-40 and sorbitol solution in a water bath at 35 ℃, uniformly mixing with the oil soluble substance in the step A, adding the water soluble substance in the step B, stirring at the temperature of 35 ℃ and the rotating speed of 500r/min until the material turns turbid from clear, and then turning the turbid to clear to obtain the oil-in-water type CBD nano-emulsion.

The seville orange flower extract is obtained by the following method: pulverizing dried flower of Rutaceae and Citrus, extracting with ethanol by conventional cold soaking, warm soaking or heating reflux extraction, filtering, recovering ethanol from the filtrate under reduced pressure, and vacuum freeze drying or spray drying the residue to obtain seville orange flower extract; wherein the mass concentration of the ethanol is 75 percent, and the adding amount of the ethanol is 10 times of the mass of the dry pollen; cold soaking at room temperature for 5 days, warm soaking at 50 deg.C for 3 hr, and extracting for 3 times; the heating reflux extraction is carried out for 1 time at 70 ℃ and each time is 30 minutes; pre-freezing for 5 hours at the condensation temperature of-50 ℃ in vacuum freeze drying, wherein the vacuum degree is 10 KPa; the air inlet temperature of spray drying is 100 ℃, and the air outlet temperature is 50 ℃.

The kapok extract is extracted by the following operations: pulverizing flos Bombacis Malabarici, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Bombacis Malabarici extract; wherein the addition amount of distilled water is 20 times of the mass of kapok pollen, cold soaking is soaking at room temperature for 5 days, warm soaking is soaking at 60 deg.C for 1 hr, and the extraction times are 2 times; the ultrasonic auxiliary extraction is carried out at 40 ℃, the ultrasonic power is 350W, the ultrasonic frequency is 20KHz, and the ultrasonic output power is 70%, the extraction times are 1 time, and each time lasts for 60 minutes; pre-freezing for 3 hours at the condensation temperature of-30 ℃ in vacuum freeze drying, wherein the vacuum degree is 5 KPa; the air inlet temperature of spray drying is 100 ℃, and the air outlet temperature is 90 ℃.

The elsholtzia extract is prepared by the following steps: pulverizing dried herba Moslae, sieving with 20 mesh sieve, adding 75% ethanol at a material-liquid ratio of 1:8, ultrasonic extracting for 3 times, 1 h/time, filtering, collecting filtrate, mixing the filtrates, vacuum filtering at night in ice bath to obtain extractive solution, recovering ethanol under reduced pressure, and concentrating on 45 deg.C water bath to obtain herba Moslae extract.

Example 2

(1) preparing materials according to the following mass ratio:

(1) fully mixing guar gum and deionized water, standing overnight in a refrigerator at 4 ℃ for full swelling to obtain a swelling liquid, adding cholesterol and 1, 2-hexanediol, carrying out ice bath ultrasonic treatment at 4 ℃, wherein the ultrasonic power is 15%, the ultrasonic on time is 10s, the ultrasonic off time is 5s, the ultrasonic duration is 15min, recovering to room temperature after the ultrasonic treatment is finished, adding potassium sorbate and CBD nano-emulsion, and carrying out constant temperature treatment at 40 ℃ for 10min in a 400r/min stirrer to obtain a material A;

(2) uniformly mixing collagen, urea, silicone, hydrogenated lecithin and a proper amount of deionized water, and stirring at 50 ℃ and the rotating speed of 300r/min until the collagen, the urea, the silicone and the hydrogenated lecithin are dissolved to obtain a material B;

The CBD nanoemulsion is prepared by the following method:

preparing materials according to the following mass ratio:

a, under the condition of keeping out of the sun, directly adding cannabidiol CBD into MCT oil and peppermint oil, heating in a water bath at the temperature of 30 ℃, stirring until the cannabidiol CBD is completely dissolved in an oil phase, and cooling to room temperature to obtain an oil solution;

and C, heating and dissolving the EL-20, EL-40 and sorbitol solution in a water bath at 30 ℃, uniformly mixing with the oil soluble substance in the step A, adding the water soluble substance in the step B, stirring at the temperature of 30 ℃ and the rotating speed of 600r/min until the material turns turbid from clear, and then turning the turbid to clear to obtain the oil-in-water type CBD nano-emulsion.

The seville orange flower extract is obtained by the following method: pulverizing dried flower of Rutaceae and Citrus, extracting with ethanol by conventional cold soaking, warm soaking or heating reflux extraction, filtering, recovering ethanol from the filtrate under reduced pressure, and vacuum freeze drying or spray drying the residue to obtain seville orange flower extract; wherein the mass concentration of the ethanol is 90 percent, and the adding amount of the ethanol is 40 times of the mass of the dry pollen; cold soaking at room temperature for 1 day, warm soaking at 40 deg.C for 1 hr, and extracting for 3 times; the heating reflux extraction is carried out for 2 times at 50 ℃ and each time for 60 minutes; pre-freezing for 5 hours at the condensation temperature of-25 ℃ in vacuum freeze drying, wherein the vacuum degree is 20 KPa; the air inlet temperature of spray drying is 300 ℃, and the air outlet temperature is 30 ℃.

The kapok extract is extracted by the following operations: pulverizing flos Bombacis Malabarici, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Bombacis Malabarici extract; wherein the addition amount of distilled water is 10 times of the mass of kapok pollen, cold soaking is soaking at room temperature for 10 days, warm soaking is soaking at 40 deg.C for 3 hours, and the extraction times are 1 time; the ultrasonic-assisted extraction is carried out at 50 ℃, the ultrasonic power of 650W, the ultrasonic frequency of 19KHz and the ultrasonic output power of 80 percent, the extraction times are 1 time, and each time lasts for 30 minutes; pre-freezing for 5 hours at the condensation temperature of-20 ℃ in vacuum freeze drying, wherein the vacuum degree is 10 KPa; the air inlet temperature of spray drying is 300 ℃, and the air outlet temperature is 140 ℃.

The elsholtzia extract is prepared by the following steps: pulverizing dried herba Moslae, sieving with 20 mesh sieve, adding 80% ethanol at a material-liquid ratio of 1:8, ultrasonic extracting for 3 times and 2 h/time, filtering, mixing filtrates, filtering at night in ice bath to obtain extractive solution, recovering ethanol under reduced pressure, and concentrating on 50 deg.C water bath to obtain herba Moslae extract.

Example 3

(1) preparing materials according to the following mass ratio:

(1) fully mixing guar gum and deionized water, standing overnight in a refrigerator at 4 ℃ for full swelling to obtain a swelling liquid, adding cholesterol and 1, 2-hexanediol, carrying out ice bath ultrasonic treatment at 4 ℃, wherein the ultrasonic power is 20%, the ultrasonic on time is 8s, the ultrasonic off time is 5s, the ultrasonic duration is 10min, recovering to room temperature after the ultrasonic treatment is finished, adding potassium sorbate and CBD nano-emulsion, and stirring at the constant temperature of 50 ℃ for 15min at a speed of 500r/min to obtain a material A;

(2) uniformly mixing collagen, urea, silicone, hydrogenated lecithin and a proper amount of deionized water, and stirring at 65 ℃ and the rotating speed of 600r/min until the collagen, the urea, the silicone and the hydrogenated lecithin are dissolved to obtain a material B;

The CBD nanoemulsion is prepared by the following method:

preparing materials according to the following mass ratio:

a, under the condition of keeping out of the sun, directly adding cannabidiol CBD into MCT oil and peppermint oil, heating in a water bath at 40 ℃, stirring until the cannabidiol CBD is completely dissolved in an oil phase, and cooling to room temperature to obtain an oil solution;

and C, heating and dissolving the EL-20, EL-40 and sorbitol solution in a water bath at 40 ℃, uniformly mixing with the oil soluble substance in the step A, adding the water soluble substance in the step B, stirring at the temperature of 40 ℃ and the rotating speed of 400r/min until the material turns turbid from clear to clear, and obtaining the oil-in-water type CBD nano-emulsion.

The seville orange flower extract is obtained by the following method: pulverizing dried flower of Rutaceae and Citrus, extracting with ethanol by conventional cold soaking, warm soaking or heating reflux extraction, filtering, recovering ethanol from the filtrate under reduced pressure, and vacuum freeze drying or spray drying the residue to obtain seville orange flower extract; wherein the mass concentration of the ethanol is 80%, and the adding amount of the ethanol is 30 times of the mass of the dry pollen; cold soaking at room temperature for 10 days, warm soaking at 60 deg.C for 2 hr, and extracting for 1 time; the heating reflux extraction is carried out for 3 times at 40 ℃ and each time for 120 minutes; pre-freezing for 10 hours at the condensation temperature of-20 ℃ in vacuum freeze drying, wherein the vacuum degree is 5 KPa; the air inlet temperature of spray drying is 250 ℃, and the air outlet temperature is 150 ℃.

The kapok extract is extracted by the following operations: pulverizing flos Bombacis Malabarici, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Bombacis Malabarici extract; wherein the addition amount of distilled water is 50 times of the mass of kapok pollen, cold soaking is soaking for 1 day at room temperature, warm soaking is soaking for 3 hours at 50 ℃, and the extraction times are 1 time; the ultrasonic-assisted extraction is carried out at the temperature of 60 ℃, the ultrasonic power of 100W, the ultrasonic frequency of 23KHz and the ultrasonic output power of 100 percent, the extraction times are 3 times, and each time lasts for 45 minutes; pre-freezing for 16 hours at the condensation temperature of-50 ℃ in vacuum freeze drying, wherein the vacuum degree is 20 KPa; the air inlet temperature of spray drying is 200 ℃, and the air outlet temperature is 30 ℃.

The elsholtzia extract is prepared by the following steps: pulverizing dried herba Moslae, sieving with 20 mesh sieve, adding 90% ethanol at a material-liquid ratio of 1:8, ultrasonic extracting for 3 times and 3 h/time, filtering, mixing the filtrates, filtering at night in ice bath to obtain extractive solution, recovering ethanol under reduced pressure, and concentrating on 40 deg.C water bath to obtain herba Moslae extract.

Example 4

(1) preparing materials according to the following mass ratio:

(1) fully mixing guar gum and deionized water, standing overnight in a refrigerator at 4 ℃ for full swelling to obtain a swelling liquid, adding cholesterol and 1, 2-hexanediol, carrying out ice bath ultrasonic treatment at 4 ℃, wherein the ultrasonic power is 15%, the ultrasonic on time is 5s, the ultrasonic off time is 8s, the ultrasonic duration is 25min, recovering to room temperature after finishing ultrasonic treatment, adding potassium sorbate and CBD nano-emulsion, and stirring at the constant temperature of 45 ℃ for 10min at a speed of 500r/min to obtain a material A;

(2) uniformly mixing collagen, urea, silicone, hydrogenated lecithin and a proper amount of deionized water, and stirring at 75 ℃ and the rotating speed of 400r/min until the collagen, the urea, the silicone and the hydrogenated lecithin are dissolved to obtain a material B;

The CBD nanoemulsion is prepared by the following method:

preparing materials according to the following mass ratio:

a, under the condition of keeping out of the sun, directly adding cannabidiol CBD into MCT oil and peppermint oil, heating in a water bath at 50 ℃, stirring until the cannabidiol CBD is completely dissolved in an oil phase, and cooling to room temperature to obtain an oil solution;

and C, heating and dissolving the EL-20, EL-40 and sorbitol solution in a water bath at 50 ℃, uniformly mixing with the oil soluble substance in the step A, adding the water soluble substance in the step B, stirring at the temperature of 50 ℃ and the rotating speed of 600r/min until the material turns turbid from clear to clear, and thus obtaining the oil-in-water type CBD nano-emulsion.

The seville orange flower extract is obtained by the following method: pulverizing dried flower of Rutaceae and Citrus, extracting with ethanol by conventional cold soaking, warm soaking or heating reflux extraction, filtering, recovering ethanol from the filtrate under reduced pressure, and vacuum freeze drying or spray drying the residue to obtain seville orange flower extract; wherein the mass concentration of the ethanol is 85 percent, and the adding amount of the ethanol is 20 times of the mass of the dry pollen; cold soaking at room temperature for 5 days, warm soaking at 55 deg.C for 3 hr, and extracting for 3 times; the heating reflux extraction is carried out for 1 time at 55 ℃ and each time for 60 minutes; pre-freezing for 10 hours at the condensation temperature of-45 ℃ in vacuum freeze drying, wherein the vacuum degree is 15 KPa; the air inlet temperature of spray drying is 220 ℃, and the air outlet temperature is 100 ℃.

The kapok extract is extracted by the following operations: pulverizing flos Bombacis Malabarici, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Bombacis Malabarici extract; wherein the addition amount of distilled water is 20 times of the mass of kapok pollen, cold soaking is soaking at room temperature for 5 days, warm soaking is soaking at 50 deg.C for 1 hr, and the extraction times are 3 times; the ultrasonic-assisted extraction is carried out at the temperature of 50 ℃, the ultrasonic power of 450W, the ultrasonic frequency of 21KHz and the ultrasonic output power of 90 percent, the extraction times are 1 time, and each time lasts for 50 minutes; pre-freezing for 5 hours at-45 ℃ in the condensation temperature of vacuum freeze drying, wherein the vacuum degree is 10 KPa; the air inlet temperature of spray drying is 300 ℃, and the air outlet temperature is 100 ℃.

The elsholtzia extract is prepared by the following steps: pulverizing dried herba Moslae, sieving with 20 mesh sieve, adding 75% ethanol at a material-liquid ratio of 1:8, ultrasonic extracting for 3 times and 2 h/time, filtering, mixing filtrates, filtering at night in ice bath to obtain extractive solution, recovering ethanol under reduced pressure, and concentrating on 45 deg.C water bath to obtain herba Moslae extract.

Example 5

(1) preparing materials according to the following mass ratio:

(1) fully mixing guar gum and deionized water, standing overnight in a refrigerator at 4 ℃ for full swelling to obtain a swelling liquid, adding cholesterol and 1, 2-hexanediol, carrying out ice bath ultrasonic treatment at 4 ℃, wherein the ultrasonic power is 15%, the ultrasonic on time is 10s, the ultrasonic off time is 5s, the ultrasonic duration is 20min, recovering to room temperature after finishing ultrasonic treatment, adding potassium sorbate and CBD nano-emulsion, and stirring at the constant temperature of 40 ℃ for 10min at the speed of 600r/min to obtain a material A;

(2) uniformly mixing collagen, urea, silicone, hydrogenated lecithin and a proper amount of deionized water, and stirring at 55 ℃ and the rotating speed of 300r/min until the collagen, the urea, the silicone and the hydrogenated lecithin are dissolved to obtain a material B;

The CBD nanoemulsion is prepared by the following method:

preparing materials according to the following mass ratio:

and C, heating and dissolving the EL-20, EL-40 and sorbitol solution in a water bath at 45 ℃, uniformly mixing with the oil soluble substance in the step A, adding the water soluble substance in the step B, stirring at the temperature of 50 ℃ and the rotating speed of 400r/min until the material turns turbid from clear to clear, and obtaining the oil-in-water type CBD nano-emulsion.

The seville orange flower extract is obtained by the following method: pulverizing dried flower of Rutaceae and Citrus, extracting with ethanol by conventional cold soaking, warm soaking or heating reflux extraction, filtering, recovering ethanol from the filtrate under reduced pressure, and vacuum freeze drying or spray drying the residue to obtain seville orange flower extract; wherein the mass concentration of the ethanol is 75 percent, and the adding amount of the ethanol is 30 times of the mass of the dry pollen; cold soaking at room temperature for 1 day, warm soaking at 70 deg.C for 3 hr, and extracting for 2 times; the heating reflux extraction is carried out for 2 times at 70 ℃ and each time is 90 minutes; pre-freezing for 5 hours at the condensation temperature of-20 ℃ in vacuum freeze drying, wherein the vacuum degree is 15 KPa; the air inlet temperature of spray drying is 256 ℃, and the air outlet temperature is 145 ℃.

The kapok extract is extracted by the following operations: pulverizing flos Bombacis Malabarici, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Bombacis Malabarici extract; wherein the addition amount of distilled water is 30 times of the mass of kapok pollen, cold soaking is soaking at room temperature for 10 days, warm soaking is soaking at 50 ℃ for 2 hours, and the extraction times are 2 times; the ultrasonic auxiliary extraction is carried out at the temperature of 45 ℃, the ultrasonic power of 550W, the ultrasonic frequency of 20KHz and the ultrasonic output power of 85 percent, the extraction times are 2 times, and each time lasts for 35 minutes; pre-freezing for 10 hours at the condensation temperature of-35 ℃ and the vacuum degree of 10KPa in vacuum freeze drying; the air inlet temperature of spray drying is 220 ℃, and the air outlet temperature is 120 ℃.

The elsholtzia extract is prepared by the following steps: pulverizing dried herba Moslae, sieving with 20 mesh sieve, adding 85% ethanol at a material-liquid ratio of 1:8, ultrasonic extracting for 3 times and 3 h/time, filtering, mixing the filtrates, filtering at ice bath overnight to obtain extractive solution, recovering ethanol under reduced pressure, and concentrating on 45 deg.C water bath to obtain herba Moslae extract.

Thirdly, the method comprises the following steps: examples of the experiments

The invention is tested by the following test examples to test the safety of the product

Experimental example I, examination of microorganisms

The detection method comprises the following steps: refer to technical Specification for safety of cosmetics 2015 year edition

Evaluation indexes are as follows: microbiological examination method according to technical Specification for safety of cosmetics 2015

Detection indexes are as follows:

index name	Standard requirements
		Total colony count CFU/ml	Less than or equal to 1000
Mould and yeast CFU/ml	Less than or equal to 100
		Thermotolerant coliform bacteria/ml	Cannot be detected
Staphylococcus aureus/ml	Cannot be detected
		Pseudomonas aeruginosa/ml	Cannot be detected

And (3) detection results:

evaluation results were as follows:

the detection result of the microorganism shows that all indexes of the microorganism accord with the technical standard for cosmetic safety, and the judgment is as follows: the results of the technical detection of the total number of bacteria and the detection of heat-resistant coliform group bacteria, staphylococcus aureus, pseudomonas aeruginosa, mold and yeast of the skin care milk containing cannabidiol CBD active ingredients and minerals in examples 1-5 all meet the national standard.

Second example tyrosinase inhibition experiment

A sample to be tested: examples 1-5 skin care milk samples containing cannabidiol CBD and minerals, commercially available ordinary skin care milk samples

Reagent: tyrosinase, absolute ethanol, PBS buffer solution

The instrument comprises the following steps: ultraviolet visible spectrophotometer and constant temperature water bath kettle

Preparing a tyrosinase solution: weighing 500U/mg tyrosinase, preparing 100U/mg enzyme solution with PBS solution, and freezing for storage.

Preparing an L-tyrosine solution: l-tyrosine and PBS buffer solution are weighed to prepare 0.05 percent solution.

Preparing a sample solution to be tested:

the method comprises the following steps: respectively placing T1, T2, C1 and C2 components in an EP tube, uniformly mixing, placing in a water bath kettle AT 37 ℃ for reaction for 10min, then adding 1ml of L-tyrosine solution into each tube, uniformly mixing, placing in a water bath kettle AT 37 ℃ for reaction for 4min, quickly placing in a cuvette, and measuring the absorbance AT 475nm of AT1, AT2, AC1 and AC 2.

The experimental results are as follows:

evaluation results were as follows:

the tyrosinase inhibition rate of the skin-care milk is increased along with the increase of the concentration of CBD (CBD) of cannabidiol, and when the concentration of CBD in the skin-care milk is more than 2%, the tyrosinase inhibition rate of the skin-care milk is greater than that of a control group skin-care milk. Thus, cannabidiol has a certain whitening effect and is closely related to concentration.

Experimental example III radical scavenging efficiency experiment

A sample to be tested: examples 1-5 skin milk samples containing cannabidiol CBD and minerals, commercially available skin milk samples

Reagent: 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH), absolute ethanol

The instrument comprises the following steps: ultraviolet visible spectrophotometer and constant temperature incubator

Preparing a sample to be tested: respectively taking 25mg of skin care milk samples containing cannabidiol CBD and mineral substances in the embodiments 1-5, dissolving the skin care milk samples with absolute ethyl alcohol, and fixing the volume to 250ml to obtain sample liquid to be detected. The product is stored in dark place, is ready for use, and is diluted into experimental concentration by absolute ethyl alcohol when in use.

Preparation of a control sample: respectively taking 25mg of a commercially available common skin care milk sample, dissolving the common skin care milk sample by using absolute ethyl alcohol, and fixing the volume to 250ml to obtain a reference sample solution. When the composition is used, absolute ethyl alcohol is used for diluting the composition into an experimental concentration.

Preparation of DPPH free radical solution: accurately weighing 25mg of DPPH, dissolving with absolute ethyl alcohol, and diluting to 250ml to obtain DPPH mother liquor, storing in dark place, and diluting with absolute ethyl alcohol to obtain experimental concentration when in use.

The method comprises the following steps: firstly, 2.5ml of absolute ethyl alcohol and 2.5ml of DPPH free radical solution are uniformly mixed and poured into a cuvette with a plug to be used as an experimental blank control. In addition, 2.5ml of a pre-prepared sample to be detected and 2.5ml of DPPH free radical solution are fully and uniformly mixed, then added into a cuvette with a plug, mixed, shaken uniformly, placed for 30min in a dark place, detected at a wavelength of 268nm by an ultraviolet spectrophotometer, and the light absorption value Ai of each sample solution is measured; the absorbance A0 of the absolute ethanol blank solution was measured.

Uniformly mixing 2.5ml of a prepared control sample with 2.5ml of DPPH free radical solution, adding the mixture into a cuvette with a plug, mixing, shaking uniformly, standing at room temperature for 30min, detecting at a wavelength of 517nm by using an ultraviolet spectrophotometer, and measuring the light absorption value Ai of each sample solution; the absorbance A0 of the absolute ethanol blank solution was measured.

Clearance [1- (Ai-Aj)/A0 ]. times.100%

Wherein: ai: absorbance measured after mixing a sample to be measured with a DPPH solution

Aj: absorbance measured after mixing control sample with DPPH solution

AO: absorbance measured after mixing of absolute ethanol with DPPH solution

Three groups of parallel samples are measured on each sample to be measured, the average value is calculated, and the result is shown in the following table:

table 1 free radical scavenging performance test results table

Evaluation results were as follows:

as can be seen from Table 1, the skin care milk prepared by the invention has a good effect of scavenging free radicals, wherein the free radical scavenging rate of the examples 2-5 is far greater than that of the control group. The traditional Chinese medicine extract can synergistically enhance the effect of the CBD nanoemulsion, and the combination of the traditional Chinese medicine extract and the CBD nanoemulsion has a good free radical scavenging effect.

Experiment example four, statistical evaluation of human trial results main reagents: skin care milk samples containing cannabidiol CBD and minerals of examples 1-5, subjects of commercially available ordinary skin care milk samples required: referring to technical Specification for safety of cosmetics 2002, a subject selects a specific experimental method:

selecting 120 women of 25-60 years old, randomly dividing into 6 groups, wherein the groups can be divided into experiment group one, experiment group two, experiment group three, experiment group four, experiment group five and control group, the gender and age degree of the women have no significant difference (P >0.05) after statistical treatment, wherein experiment group 1 corresponds to experiment example 1, experiment group 2 corresponds to experiment example 2, and the like)

② the treatment method comprises: the skin care lotion samples containing the cannabidiol CBD nanoemulsion obtained in examples 1-5 are smeared on the skin care lotion samples for 30 days, 60 days and 90 days respectively for effect statistics, and the skin is thoroughly cleaned in the morning and evening and then smeared on the face for 2 times a day; the control group was applied with a commercial ordinary skin care cream product for 30 days, 60 days, 90 days, and then applied 2 times a day after thoroughly cleaning the skin in the morning and evening.

Evaluation index:

the method has the following advantages: the product is used for 30 days, and compared with the product before use, the product has the advantages of reduced speckle and improved darkness;

when the skin care product is used for 60 days, the skin color is brightened, and the skin is uniformly white;

after 90 days of use, the color spots disappear, and the skin is ruddy and white.

And (4) invalidation: after the composition is used for 30 days, compared with the composition before use, the spots have no sign of fading, and darkness is not improved to be ineffective;

after the skin-care product is used for 60 days, the skin color has no brightening sign, and the skin color is not uniform and ineffective;

the stain was still present as ineffective after 90 days of use.

Evaluation results were as follows:

as shown in the data analysis of the table 2, the comparison between the whitening, antioxidant and speckle-reducing skin care milk of the invention and the skin care milk of a control group shows that the skin care milk of the invention has more effective people in the same time, and further shows that the skin care milk of the invention has better whitening and speckle-reducing results.

According to the feedback of volunteers, the skin-care cream provided by the invention can greatly improve the whitening effect, is relatively gentle, can improve the adverse phenomena of skin darkness and the like, and after 97% of volunteers react for 90 days in an experiment, spots on the face obviously disappear, the skin becomes ruddy, white, tender and elastic, and the skin-care cream is beneficial to better fitting of other cosmetics.

Claims

1. A skin care emulsion containing cannabidiol nanoemulsion is characterized by being prepared from the following components in percentage by mass:

10-50% of CBD nanoemulsion

0.5 to 3 percent of seville orange flower extract

0.5 to 3 percent of common bombax flower extract

0.3 to 5 percent of elsholtzia extract

1% -5% of hydrogenated lecithin

1 to 10 percent of silicone

1% -5% of collagen

1 to 8 percent of urea

0.01 to 0.03 percent of potassium sorbate

0.01 to 0.05 percent of guar gum

0.1% -0.5% of cholesterol

0.5 to 3 percent of 1, 2-hexanediol

Balance of deionized water

The sum of the above components is 100%.

2. The skin care emulsion comprising a cannabidiol nanoemulsion of claim 1, wherein the CBD nanoemulsion was prepared by the following method:

preparing materials according to the following mass ratio:

MCT oil 1-15%

Peppermint oil 0.5-5%

EL-20 1-5%

EL-40 1-10%

1-10% of sorbitol solution

Cannabidiol CBD 1-20%

Mineral concentrated solution 0.1-20%

Balance of deionized water

The sum of the components is 100 percent;

3. The skin care emulsion comprising cannabidiol nanoemulsion of claim 1, wherein the seville orange flower extract is obtained by the following method: pulverizing dried flower of Rutaceae and Citrus, extracting with ethanol by conventional cold soaking, warm soaking or heating reflux extraction, filtering, recovering ethanol from the filtrate under reduced pressure, and vacuum freeze drying or spray drying the residue to obtain seville orange flower extract; wherein the mass concentration of the ethanol is 75-90%, and the adding amount of the ethanol is 10-40 times of the mass of the dry pollen; the cold soaking is carried out for 1-10 days at room temperature, the warm soaking is carried out for 1-3 hours at the temperature of 40-70 ℃, and the extraction times are 1-3 times; the heating reflux extraction is carried out for 1-3 times at 40-70 ℃, and each time lasts for 30-120 minutes; pre-freezing for 5-10 hours at the condensation temperature of-20 to-50 ℃ and the vacuum degree of 5-20 KPa in vacuum freeze drying; the air inlet temperature of spray drying is 100-300 ℃, and the air outlet temperature is 30-150 ℃.

4. The skin care emulsion comprising cannabidiol nanoemulsion of claim 1, wherein the kapok extract is extracted by: pulverizing flos Bombacis Malabarici, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Bombacis Malabarici extract; wherein the adding amount of the distilled water is 10-50 times of the mass of the ceiba pollen, the cold soaking is to soak for 1-10 days at room temperature, the warm soaking is to soak for 1-3 hours at 40-60 ℃, and the extraction times are 1-3 times; the ultrasonic-assisted extraction is carried out at the temperature of 40-60 ℃, the ultrasonic power of 100-650W, the ultrasonic frequency of 19-23 KHz and the ultrasonic output power of 70-100%, the extraction times are 1-3, and each time lasts for 30-60 minutes; pre-freezing for 3-16 hours at the condensation temperature of-20 to-50 ℃ in vacuum freeze drying, wherein the vacuum degree is 5-20 KPa; the air inlet temperature of spray drying is 100-300 ℃, and the air outlet temperature is 30-140 ℃.

5. The skin care emulsion comprising cannabidiol nanoemulsion of claim 1, wherein the elsholtzia extract is prepared by the following method: pulverizing dried herba Moslae, sieving with 20 mesh sieve, adding 75-90% ethanol at a material-liquid ratio of 1:8, ultrasonic extracting for 3 times, 1-3 h/time, filtering, mixing filtrates, mixing 3 times of filtrates, performing suction filtration overnight in ice bath to obtain extractive solution, recovering ethanol under reduced pressure, and concentrating on 40-50 deg.C water bath to obtain herba Moslae extract.

6. The skin care emulsion comprising cannabidiol nanoemulsion of claim 1, prepared by the steps of: