CN115485386B - 低生物碱含量的烟草属植物体及其制备方法 - Google Patents

低生物碱含量的烟草属植物体及其制备方法 Download PDF

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Publication number
CN115485386B
CN115485386B CN202180028659.6A CN202180028659A CN115485386B CN 115485386 B CN115485386 B CN 115485386B CN 202180028659 A CN202180028659 A CN 202180028659A CN 115485386 B CN115485386 B CN 115485386B
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gene
mutation
tobacco
polypeptide
seq
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CN115485386A (zh
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竹内贵规
新井雅雄
宇田川久史
真笼洋
西口辽
高仓由光
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Japan Tobacco Inc
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Japan Tobacco Inc
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823—Nicotiana, e.g. tobacco
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
    • A01H1/101—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine or caffeine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10—Chemical features of tobacco products or tobacco substitutes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/03—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12Y104/03016—L-Aspartate oxidase (1.4.3.16)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • Physiology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202180028659.6A 2020-04-17 2021-04-19 低生物碱含量的烟草属植物体及其制备方法 Active CN115485386B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020-074233 2020-04-17
JP2020074233 2020-04-17
PCT/JP2021/015920 WO2021210688A1 (ja) 2020-04-17 2021-04-19 低アルカロイド含量のタバコ属植物体およびその製造方法

Publications (2)

Publication Number Publication Date
CN115485386A CN115485386A (zh) 2022-12-16
CN115485386B true CN115485386B (zh) 2024-03-29

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Country Link
US (1) US12239090B2 (https=)
EP (1) EP4136964A4 (https=)
JP (1) JP7210806B2 (https=)
CN (1) CN115485386B (https=)
BR (1) BR112022021066A2 (https=)
WO (1) WO2021210688A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112022021375A2 (pt) * 2020-04-21 2022-12-06 Japan Tobacco Inc Corpo da planta de tabaco e método para produzir o mesmo
CN113528537A (zh) * 2021-08-11 2021-10-22 云南省烟草农业科学研究院 一种降低烟叶烟碱含量的NtQPT2基因突变体及其应用
CN118475234A (zh) * 2021-12-27 2024-08-09 日本烟草产业株式会社 烟草植物

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101155920A (zh) * 2005-02-28 2008-04-02 奈良先端科学技术大学院大学 植物中烟碱生物碱的水平的降低
CN106459923A (zh) * 2014-05-08 2017-02-22 菲利普莫里斯产品有限公司 植物中烟碱向降烟碱转化的减少
CN108882689A (zh) * 2016-03-30 2018-11-23 日本烟草产业株式会社 烟草植物体及其制备方法
WO2023015712A1 (zh) * 2021-08-10 2023-02-16 深圳瑞德林生物技术有限公司 一种s-尼古丁的制备方法

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Publication number Priority date Publication date Assignee Title
EP2537929B1 (en) 2010-02-17 2017-04-12 Japan Tobacco, Inc. Plant component regulation factor, and use thereof
KR20180085794A (ko) * 2015-12-04 2018-07-27 노쓰 캐롤라이나 스테이트 유니버시티 담배 베르베린 브릿지 효소-유사 핵산의 표적화된 돌연변이생성
US20180340180A1 (en) * 2017-05-19 2018-11-29 22Nd Century Limited, Llc Dominant-negative genetic manipulation to make low-nicotine tobacco products
WO2018222667A1 (en) * 2017-05-31 2018-12-06 22Nd Century Limited, Llc Genome editing methods for producing low-nicotine tobacco products
US10812495B2 (en) 2017-10-06 2020-10-20 Uvic Industry Partnerships Inc. Secure personalized trust-based messages classification system and method
EP4069854A1 (en) * 2019-12-03 2022-10-12 Altria Client Services LLC Compositions and methods for producing tobacco plants and products having altered alkaloid levels

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101155920A (zh) * 2005-02-28 2008-04-02 奈良先端科学技术大学院大学 植物中烟碱生物碱的水平的降低
CN106367434A (zh) * 2005-02-28 2017-02-01 二十二世纪有限公司 降低植物中生物碱的方法、遗传工程植物细胞以及降低了生物碱的产品
CN106459923A (zh) * 2014-05-08 2017-02-22 菲利普莫里斯产品有限公司 植物中烟碱向降烟碱转化的减少
CN108882689A (zh) * 2016-03-30 2018-11-23 日本烟草产业株式会社 烟草植物体及其制备方法
WO2023015712A1 (zh) * 2021-08-10 2023-02-16 深圳瑞德林生物技术有限公司 一种s-尼古丁的制备方法

Non-Patent Citations (5)

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Title
"Generation of tobacco lines with widely different reduction in nicotine levels via RNA silencing approaches";PENG WANG等;《J. Biosci》;第33卷(第2期);第177-184页 *
"Genetic attenuation of alkaloids and nicotine content in tobacco (Nicotiana tabacum)";Diego Hidalgo Martinez等;《Planta》;第251卷(第92期);第1-14页 *
Diego Hidalgo Martinez等."Genetic attenuation of alkaloids and nicotine content in tobacco (Nicotiana tabacum)".《Planta》.2020,第251卷(第92期),第1-14页. *
Ramsey S. Lewis."Potential Mandated Lowering of Nicotine Levels in Cigarettes: A Plant Perspective".2018,第1-15页. *
Sana Khan等."Cloning and functional characterization of quinolinic acid phosphoribosyl transferase (QPT) gene of Nicotiana tabacum".2016,第1-27页. *

Also Published As

Publication number Publication date
JP7210806B2 (ja) 2023-01-23
JPWO2021210688A1 (https=) 2021-10-21
US12239090B2 (en) 2025-03-04
EP4136964A1 (en) 2023-02-22
CN115485386A (zh) 2022-12-16
US20230120622A1 (en) 2023-04-20
EP4136964A4 (en) 2024-05-22
WO2021210688A1 (ja) 2021-10-21
BR112022021066A2 (pt) 2022-12-06

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