CN115463170A

CN115463170A - Traditional Chinese medicine composition for promoting estrus of livestock and poultry, feed additive, and preparation method and application thereof

Info

Publication number: CN115463170A
Application number: CN202211010438.9A
Authority: CN
Inventors: 李建涛; 刘显军; 李新; 张宇宏; 韩淑敏
Original assignee: Shenyang Agricultural University
Current assignee: Shenyang Agricultural University
Priority date: 2022-08-23
Filing date: 2022-08-23
Publication date: 2022-12-13
Anticipated expiration: 2042-08-23
Also published as: CN115463170B

Abstract

The invention relates to the field of livestock and poultry breeding, in particular to a traditional Chinese medicine composition for promoting livestock and poultry estrus, a feed additive, and a preparation method and application thereof. The invention provides a traditional Chinese medicine composition for promoting oestrus of livestock and poultry, which comprises the following components in parts by mass: 15 to 25 portions of epimedium, 15 to 25 portions of dodder, 15 to 25 portions of astragalus, 35 to 45 portions of angelica, 15 to 25 portions of motherwort and 5 to 15 portions of liquorice. The traditional Chinese medicine composition can well solve the stress problem of livestock, and particularly can improve the oestrus rate and pregnancy rate of the livestock, shorten the oestrus interval and improve the reproductive performance in the aspect of livestock reproduction.

Description

Traditional Chinese medicine composition for promoting estrus of livestock and poultry, feed additive and preparation method and application thereof

Technical Field

The invention relates to the field of livestock breeding, in particular to a traditional Chinese medicine composition for promoting the estrus of livestock, a feed additive, a preparation method and application thereof.

Background

With the rapid development of animal husbandry and the implementation of resistance policy banning, chinese herbal medicines are used as one of the alternative products due to the characteristics of natural sources, small toxic and side effects, no drug resistance, safety, reliability, special biological functions and the like. The Chinese medicinal materials contain polysaccharides, flavonoids, saponins, polyphenols, alkaloids, and anthrones. In addition, it also contains amino acids, proteins, vitamins, minerals and trace elements. Some scholars use Chinese herbal medicines in production practice to improve the pregnancy rate of livestock and poultry and promote the oestrus and pregnancy of livestock and poultry.

With the continuous and deep research on the functions and pharmacological action mechanisms of Chinese herbal medicines, the Chinese herbal medicines are increasingly widely added and used in the animal breeding process. Most of the traditional Chinese medicine compound preparations are decocted or ground into superfine powder and then added into daily ration for feeding, so that the utilization rate of Chinese herbal medicines is low, and the traditional Chinese medicine composition in the prior art cannot well solve the problem of oestrus of livestock.

Disclosure of Invention

In order to solve the problems, the invention provides a traditional Chinese medicine composition for promoting oestrus of livestock and poultry, a feed additive and application thereof. The traditional Chinese medicine composition can well solve the stress problem of livestock, and particularly can improve the oestrus rate and pregnancy rate of the livestock, shorten the oestrus interval and improve the reproductive performance in the aspect of livestock reproduction.

The invention also provides a preparation method of the traditional Chinese medicine composition, and the preparation method can improve the content of effective active ingredients and improve the drug effect and the utilization rate.

In order to achieve the above purpose, the invention provides the following technical scheme:

the invention provides a traditional Chinese medicine composition for promoting oestrus of livestock and poultry, which comprises the following components in parts by mass:

15 to 25 portions of epimedium, 15 to 25 portions of dodder, 15 to 25 portions of astragalus, 35 to 45 portions of angelica, 15 to 25 portions of motherwort and 5 to 15 portions of liquorice.

The invention provides a preparation method of the traditional Chinese medicine composition in the technical scheme, which comprises the following steps:

mixing herba Epimedii, semen Cuscutae, radix astragali, radix Angelicae sinensis, herba Leonuri and Glycyrrhrizae radix to obtain Chinese medicinal composition.

Preferably, the dodder is a product subjected to enzymolysis treatment; the astragalus is a product treated by enzymolysis; the epimedium is a product subjected to enzymolysis treatment, microbial fermentation treatment or bacterium-enzyme synergistic fermentation treatment; the angelica is a product subjected to enzymolysis treatment, microbial fermentation treatment or bacterial enzyme synergistic fermentation treatment; the liquorice is a product obtained by enzymolysis, microbial fermentation or bacterial enzyme synergistic fermentation; the herba Leonuri is untreated herba Leonuri or product obtained by microbial fermentation.

Preferably, when the epimedium herb is a product subjected to enzymolysis, the angelica is a product subjected to bacterial enzyme synergistic fermentation treatment, the licorice is a product subjected to enzymolysis, and the leonurus is an untreated leonurus.

Preferably, during the enzymolysis treatment, the enzyme preparation adopted is a complex enzyme preparation, and the complex enzyme preparation comprises xylanase, cellulase and laccase; the activity of the xylanase is 8000u/g, the activity of the cellulase is 30u/g, and the activity of the laccase is 4000u/g; the enzymolysis treatment time is 6-12 h, and the temperature is 50-60 ℃.

Preferably, during the microbial fermentation treatment, the adopted microbial agents comprise bacillus licheniformis, bacillus coagulans and aspergillus niger; the time of the microbial fermentation is 3-5 days, and the temperature is 30-40 ℃.

Preferably, when the bacterial enzymes are subjected to synergistic fermentation treatment, the enzyme preparation adopted is a complex enzyme preparation, and the complex enzyme preparation comprises xylanase, cellulase and laccase; the activity of the xylanase is 8000u/g, the activity of the cellulase is 30u/g, and the activity of the laccase is 4000u/g; when the bacterial enzymes are used for fermentation in a synergistic manner, the adopted microbial inoculum comprises bacillus licheniformis, bacillus coagulans and aspergillus niger; the time of the synergistic fermentation of the bacterial enzymes is 3-5 days, and the temperature is 30-40 ℃.

The invention provides a feed additive for promoting oestrus of livestock and poultry, and the effective components of the feed additive comprise the traditional Chinese medicine composition in the technical scheme or the traditional Chinese medicine composition obtained by the preparation method in the technical scheme.

The invention provides application of the traditional Chinese medicine composition in the technical scheme, the traditional Chinese medicine composition obtained by the preparation method in the technical scheme, or the feed additive in the technical scheme in any one or more of improving the oestrus rate of livestock and poultry, improving the pregnancy rate of livestock and poultry, shortening oestrus intervals and improving reproductive performance.

Has the beneficial effects that:

the invention provides an anti-stress traditional Chinese medicine composition for livestock, which comprises the following components in parts by mass: 15 to 25 portions of epimedium, 15 to 25 portions of dodder, 15 to 25 portions of astragalus, 35 to 45 portions of angelica, 15 to 25 portions of motherwort and 5 to 15 portions of liquorice. According to the invention, the epimedium and the dodder have the main functions of tonifying kidney and strengthening yang as monarch drugs, the astragalus mongholicus and the angelica sinensis are ministerial drugs for replenishing blood and tonifying qi, the motherwort is adjuvant drugs for promoting blood circulation and removing blood stasis, the liquorice is used for clearing heat and removing toxicity, and the balanced drug property is messenger drugs, so that the stress response of livestock can be well relieved, the utilization rate of traditional Chinese medicines is improved, the oestrus rate and pregnancy rate of the livestock can be improved, the oestrus interval is shortened, and the reproductive performance is improved.

The invention carries out certain pretreatment on the traditional Chinese medicine, and can obviously improve the efficacy of the traditional Chinese medicine composition. The results of the specific examples of the present invention show that: the pretreated traditional Chinese medicine composition can obviously improve the oestrus rate and pregnancy rate of replacement gilts, can improve the oestrus rate and pregnancy rate of multiparous sows, and obviously shortens oestrus intervals. And can obviously improve the contents of progestogen, estrogen, luteinizing hormone and follicle stimulating hormone of replacement sows and multiparous sows.

In addition, the composition is compounded, so that the drug effect can be obviously improved, and the effect of saving the cost is achieved while the drug effect is ensured.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments will be briefly described below.

FIG. 1 is a standard graph of polysaccharides;

FIG. 2 is a standard graph of flavones.

Detailed Description

The components of the present invention are all obtained by ordinary purchase of those skilled in the art, unless otherwise specified.

The invention provides a traditional Chinese medicine composition for promoting oestrus of livestock and poultry, which comprises the following components in parts by mass: 15 to 25 portions of epimedium, 15 to 25 portions of dodder, 15 to 25 portions of astragalus, 35 to 45 portions of angelica, 15 to 25 portions of motherwort and 5 to 15 portions of liquorice.

The traditional Chinese medicine composition comprises, by mass, 15-25 parts of epimedium, preferably 16-24 parts, more preferably 17-22 parts, and most preferably 20 parts. In the present invention, the epimedium is preferably a product treated by enzymolysis, microbial fermentation or fungal enzyme synergistic fermentation, and more preferably a product treated by microbial fermentation.

Based on the mass parts of epimedium, the traditional Chinese medicine composition comprises 15-25 parts of semen cuscutae, preferably 17-24 parts of semen cuscutae. More preferably from 18 to 23 parts, and most preferably 20 parts. In the present invention, the dodder is preferably an enzymolysis-treated product.

Based on the mass parts of epimedium, the traditional Chinese medicine composition comprises 15-25 parts of astragalus, preferably 16-23 parts, more preferably 18-21 parts, and most preferably 20 parts. In the present invention, the astragalus is preferably a product subjected to enzymatic treatment.

Based on the mass parts of epimedium, the traditional Chinese medicine composition comprises 35-45 parts of angelica, preferably 36-44 parts, more preferably 39-43 parts, and most preferably 40 parts. In the invention, the angelica is preferably a product after enzymolysis, microbial fermentation and bacterial enzyme synergistic fermentation treatment, and more preferably a product after bacterial enzyme synergistic fermentation treatment.

Based on the mass parts of epimedium, the traditional Chinese medicine composition comprises 15-25 parts of motherwort, preferably 16-24 parts, more preferably 18-21 g parts, and most preferably 20 parts. In the present invention, the motherwort is preferably untreated motherwort or a product of fermentation treatment by a microorganism, more preferably untreated motherwort.

Based on the mass parts of epimedium, the traditional Chinese medicine composition of the invention comprises 5 to 15 parts of liquorice, preferably 6 to 13 parts, more preferably 8 to 11 parts, and most preferably 10 parts. In the present invention, the licorice is preferably a product of enzymolysis, microbial fermentation and bacterial enzyme synergistic fermentation, and more preferably a product of enzymolysis treatment.

According to the traditional Chinese medicine composition, the epimedium and the dodder have the main functions of tonifying kidney and strengthening yang as monarch drugs, the astragalus mongholicus and the angelica sinensis are ministerial drugs for replenishing blood and tonifying qi, the motherwort is adjuvant drugs for promoting blood circulation and removing blood stasis, the liquorice is capable of clearing heat and removing toxicity, the drug property is balanced as a guiding drug, stress response of livestock and poultry can be well relieved, effective components in the treated traditional Chinese medicines are increased, the six traditional Chinese medicines are compatible and reasonably matched, a synergistic effect can be generated, the effect of the utilization rate of the traditional Chinese medicines is further improved, meanwhile, the oestrus rate and pregnancy rate of the livestock and the oestrus interval can be improved, and the reproductive performance is improved.

In the present invention, the livestock preferably includes ruminants, swine or poultry; the ruminants preferably comprise ruminants such as cattle and sheep, and the poultry comprises poultries such as broilers, laying hens and ducks. It can be used for poultry, and also has effects of improving oviduct.

In the present invention, the dodder is preferably an enzymolysis-treated product; the astragalus is preferably a product subjected to enzymolysis treatment; the epimedium is preferably a product subjected to enzymolysis treatment, microbial fermentation treatment or bacterium enzyme synergistic fermentation treatment, and more preferably a product subjected to microbial fermentation treatment; the angelica is preferably a product obtained by enzymolysis, microbial fermentation or bacterial enzyme synergistic fermentation; more preferably, the product is treated by bacterial enzyme synergistic fermentation; the licorice is preferably a product obtained by enzymolysis, microbial fermentation or bacterial enzyme synergistic fermentation, and more preferably a product obtained by enzymolysis; the motherwort is preferably untreated motherwort or a product obtained by fermentation treatment of a microorganism, and more preferably untreated motherwort. When different traditional Chinese medicine raw materials are subjected to enzymolysis treatment, the enzymolysis treatment conditions can be selected and have the same condition range; when different traditional Chinese medicine raw materials are subjected to microbial fermentation treatment, the conditions and the ranges of the microbial fermentation treatment modes can be selected and are preferably the same; when different traditional Chinese medicine raw materials are subjected to bacterium-enzyme synergistic fermentation treatment, the conditions and the ranges of the selectable bacterium-enzyme synergistic fermentation treatment modes are preferably the same.

Taking the dodder as an example, a compound enzyme preparation is preferably adopted during the enzymolysis treatment; the compound enzyme preparation preferably comprises xylanase, cellulase and laccase; the activity of the xylanase is preferably 8000u/g, the activity of the cellulase is preferably 30u/g, and the activity of the laccase is preferably 4000u/g. In the invention, the time of the enzymolysis treatment is preferably 6 to 12 hours, more preferably 6 to 10 hours, more preferably 6 to 8 hours, and most preferably 7 hours; the temperature of the enzymolysis treatment is preferably 50-60 ℃, more preferably 52-58 ℃, and most preferably 55 ℃. The addition amount of the complex enzyme preparation is preferably 5kg/t based on the mass of the semen cuscutae with initial moisture. In the present invention, the water content of the dodder seed is preferably 40 to 50wt.%, more preferably 43 to 48wt.%, and most preferably 45wt.% during the enzymatic hydrolysis treatment. The source of the complex enzyme preparation is not particularly limited, and the complex enzyme preparation can be obtained by conventional purchase by a person skilled in the art; in a specific embodiment of the invention, the complex enzyme preparation is preferably purchased from santong saury biosciences ltd. The quality of the semen cuscutae with initial moisture according to the present invention preferably refers to the quality of the semen cuscutae when the semen cuscutae is purchased.

Before the enzymolysis treatment, the invention also preferably comprises the steps of crushing and sieving the dodder; the invention has no requirements on the crushing mode, and can adopt the mode which is well known by the person skilled in the art; the screening is preferably 60 to 120 mesh, more preferably 80 mesh.

After the enzymolysis treatment, the invention also preferably comprises drying the product obtained by the enzymolysis treatment of the dodder; the drying temperature is preferably 40-60 ℃, and most preferably 55 ℃; the drying finishing standard is preferably that the water content of a dried product obtained after drying reaches the initial moisture; the initial moisture content of the present invention is detailed in table 1.

The conditions of the enzymolysis treatment process and the crushing and sieving of the astragalus, the epimedium, the angelica and the liquorice are the same as those of the dodder.

The specific enzyme preparation adopted by the invention can set the temperature, time and moisture of specific enzymolysis according to the characteristics of the enzyme preparation, so that the effective components of the traditional Chinese medicine can be improved.

Taking epimedium as an example, the microbial inoculum adopted during the microbial fermentation treatment of the invention preferably comprises bacillus licheniformis, bacillus coagulans and aspergillus niger. The addition amount of the microbial inoculum during the microbial fermentation treatment is preferably 5 × 10 based on the mass of the herba Epimedii with initial moisture ⁷ CFU/g; the bacterial activity of the bacillus licheniformis in the microbial inoculum is preferably 1000 hundred million CFU/g, the bacterial activity of the bacillus coagulans is preferably 100 hundred million CFU/g, and the bacterial activity of the aspergillus niger is preferably 1000 hundred million CFU/g. In the invention, the formulation of the microbial inoculum is preferably powder; the bacillus licheniformis, bacillus coagulans and aspergillus niger are preferably in the form of bacterial powder; the mass ratio of the bacillus licheniformis powder, the bacillus coagulans powder and the aspergillus niger powder in the microbial agent is preferably 1; the bacteria activity ratio of the bacillus licheniformis, the bacillus coagulans and the aspergillus niger in the microbial inoculum is preferably 1. The time for the microbial fermentation treatment is preferably 3-5 d, and more preferably 5d; the temperature of the microbial fermentation treatment is preferably 30 to 40 ℃, more preferably 32 to 37 ℃, and most preferably 35 ℃. In the present invention, the water content of epimedium herb is preferably 40 to 50wt.%, more preferably 43 to 48wt.%, and most preferably 45wt.% during the microbial fermentation treatment. The quality of the epimedium herb with initial moisture preferably refers to the quality of the epimedium herb when the epimedium herb is purchased.

Before the microbial fermentation treatment, the invention also preferably comprises the steps of crushing and sieving the epimedium; the invention has no requirement on the crushing mode, and the mode which is familiar to the person skilled in the art can be adopted; the screening is preferably 60 to 120 mesh, more preferably 80 mesh.

After the microbial fermentation treatment, the invention also preferably comprises the step of drying a product obtained by the microbial fermentation treatment of the south dodder seed; the drying temperature is preferably 30-40 ℃, and most preferably 35 ℃; the drying finishing standard is preferably that the water content of a dried product obtained after drying reaches the initial water content; the initial moisture of the invention is detailed in table 1.

The microbial fermentation treatment process and the crushing and sieving conditions of the angelica and the liquorice are the same as those of the epimedium.

The special microorganism adopted by the invention sets the fermentation temperature, time and moisture of the special microorganism according to the characteristics of the microorganism, can avoid the inactivation of the microorganism caused by overhigh temperature, and can improve the effective components of the traditional Chinese medicine.

Taking epimedium as an example, when the bacterial enzymes are subjected to synergistic fermentation treatment, the adopted microbial inoculum preferably comprises bacillus licheniformis, bacillus coagulans and aspergillus niger. The addition amount of the microbial inoculum during microbial fermentation is preferably 5 × 10 based on the mass of the epimedium with initial moisture ⁷ CFU/g; the bacterial activity of bacillus licheniformis in the microbial inoculum is preferably 1000 hundred million CFU/g, the bacterial activity of bacillus coagulans is preferably 100 hundred million CFU/g, and the bacterial activity of aspergillus niger is preferably 1000 hundred million CFU/g; in the invention, the formulation of the microbial inoculum is preferably powder; the bacillus licheniformis, bacillus coagulans and aspergillus niger are preferably in the form of bacterial powder; the mass ratio of the bacillus licheniformis powder, the bacillus coagulans powder and the aspergillus niger powder in the microbial inoculum is preferably 1; the bacteria activity ratio of the bacillus licheniformis, the bacillus coagulans and the aspergillus niger in the microbial inoculum is preferably 1. The invention preferably adopts a complex enzyme preparation; the compound enzyme preparation preferably comprises xylanase, cellulase and laccase; the activity of the xylanase is preferably 8000u/g, the activity of the cellulase is preferably 30u/g, and the activity of the laccase is preferably 4000u/g in each g of the complex enzyme preparation. The addition amount of the complex enzyme preparation is preferably 5kg/t based on the mass of the epimedium with initial moisture. The time of the bacterial enzyme synergistic fermentation treatment is preferably 3 to 5 days, and more preferably 5 days; the bacterial enzymes cooperateThe temperature of the fermentation treatment is preferably 30 to 40 ℃, more preferably 32 to 37 ℃, and most preferably 35 ℃. In the present invention, when the bacteria and enzymes are used for synergistic fermentation, the water content of epimedium is preferably 40 to 50wt.%, more preferably 43 to 48wt.%, and most preferably 45wt.%. The quality of the epimedium herb with initial moisture preferably refers to the quality of the epimedium herb when the epimedium herb is purchased.

Before the fungus-enzyme synergistic fermentation treatment, the invention also preferably comprises the condition range of crushing and sieving the epimedium; the invention has no requirement on the crushing mode, and the mode which is familiar to the person skilled in the art can be adopted; the screening is preferably 60 to 120 mesh, more preferably 80 mesh.

After the bacterial enzyme synergistic fermentation treatment, the invention also preferably comprises drying a product obtained by the semen cuscutae bacterial enzyme synergistic fermentation treatment; the drying temperature is preferably 30-40 ℃, and most preferably 35 ℃; the drying finishing standard is preferably that the water content of a dried product obtained after drying reaches the initial water content; the initial moisture content of the present invention is detailed in table 1.

The sources of the bacillus licheniformis, the bacillus coagulans and the aspergillus niger are not particularly limited, and the bacillus licheniformis, the bacillus coagulans and the aspergillus niger can be obtained by conventional purchase by a person skilled in the art; in a specific embodiment of the present invention, the bacillus licheniformis and bacillus coagulans are preferably obtained from Qingdao root-source biotechnology collective, ltd, and the aspergillus niger is preferably obtained from Kangyuan biotechnology, ltd.

The bacterial enzyme synergistic fermentation treatment process and crushing and sieving condition parameters of the angelica and the liquorice are the same as those of the epimedium.

The special enzyme preparation and microorganism are adopted by the invention, and the temperature, time and moisture of special fermentation are set according to the characteristics of the enzyme preparation and the microorganism, so that the microorganism inactivation caused by overhigh temperature can be avoided, and meanwhile, the effective components of the traditional Chinese medicine can be improved.

In the composition, the semen cuscutae is preferably a product subjected to enzymolysis, the astragalus membranaceus is preferably a product subjected to enzymolysis, and when the epimedium is preferably a product understood by enzymes, the angelica sinensis is preferably a product subjected to bacterial enzyme synergistic fermentation, the licorice is preferably a product subjected to enzymolysis, and the leonurus is preferably an untreated leonurus;

or, in the composition, the semen cuscutae is preferably a product subjected to enzymolysis, the astragalus is preferably a product subjected to enzymolysis, the epimedium is a product subjected to enzymolysis, and the angelica is preferably a product subjected to enzymolysis; the licorice is preferably a product subjected to enzymolysis treatment; the herba Leonuri is preferably untreated herba Leonuri;

or, in the composition, the semen cuscutae is preferably an enzymolysis product, the astragalus is preferably an enzymolysis product, the epimedium is an enzymolysis product, the angelica is preferably an enzymolysis product, the licorice is preferably an enzymolysis product, and the leonurus is preferably a product subjected to microbial fermentation;

or in the composition, the semen cuscutae is preferably a product subjected to enzymolysis, the astragalus membranaceus is preferably a product subjected to enzymolysis, and when the epimedium is preferably a product subjected to enzymolysis, the angelica sinensis is preferably a product subjected to bacterial enzyme synergistic fermentation, the licorice is preferably a product subjected to enzymolysis, and the leonurus is preferably a product subjected to microbial fermentation;

or, in the composition, the semen cuscutae is preferably a product subjected to enzymolysis, the astragalus is preferably a product subjected to enzymolysis, the epimedium is a product subjected to microbial fermentation, the angelica is a product subjected to microbial fermentation, the licorice is a product subjected to microbial fermentation, and the leonurus is untreated leonurus;

or, in the composition, the semen cuscutae is preferably a product subjected to enzymolysis, the astragalus is preferably a product subjected to enzymolysis, the epimedium is a product subjected to microbial fermentation, the angelica is a product subjected to microbial fermentation, the licorice is a product subjected to microbial fermentation, and the leonurus is a product subjected to microbial fermentation;

or, in the composition, the semen cuscutae is preferably a product subjected to enzymolysis, the astragalus is preferably a product subjected to enzymolysis, the epimedium is a product subjected to bacterial enzyme synergistic fermentation, the angelica is a product subjected to bacterial enzyme synergistic fermentation, the liquorice is a product subjected to bacterial enzyme synergistic fermentation, and the leonurus is a product subjected to microbial fermentation;

or in the composition, the semen cuscutae is preferably a product subjected to enzymolysis, the astragalus membranaceus is preferably a product subjected to enzymolysis, the epimedium herb is a product subjected to bacterium-enzyme synergistic fermentation, the angelica sinensis is a product subjected to bacterium-enzyme synergistic fermentation, the licorice root is a product subjected to bacterium-enzyme synergistic fermentation, and the leonurus is untreated leonurus.

According to the characteristics of the traditional Chinese medicine composition, the invention provides the feed additive for promoting the estrus of livestock and poultry, and the effective components of the feed additive comprise the traditional Chinese medicine composition in the technical scheme or the traditional Chinese medicine composition obtained by the preparation method in the technical scheme. The type of feed additive according to the invention is preferably a mixed feed additive.

The pretreated traditional Chinese medicine composition provided by the invention can obviously improve the oestrus rate and pregnancy rate of replacement gilts, can improve the oestrus rate and pregnancy rate of multiparous sows, and obviously shortens oestrus intervals. And the contents of progestogen, estrogen, luteinizing hormone and follicle stimulating hormone of replacement gilts and multiparous sows can be obviously improved, so the traditional Chinese medicine composition can be used for improving the oestrus rate of the sows, improving the pregnancy rate of the sows, shortening the oestrus interval and improving the reproductive performance.

According to the advantages of the traditional Chinese medicine composition, the invention provides the application of the traditional Chinese medicine composition in the technical scheme, the traditional Chinese medicine composition obtained by the preparation method in the technical scheme, or the feed additive in the technical scheme in one or more of improving the oestrus rate of livestock, improving the pregnancy rate of livestock, shortening the oestrus interval and improving the reproductive performance. In the present invention, the livestock preferably includes ruminants, swine or poultry; the ruminants comprise ruminants such as cattle and sheep, and the poultry comprise poultry such as broiler chickens, laying hens and ducks.

For further illustration of the present invention, the following detailed descriptions of the traditional Chinese medicine composition for promoting oestrus of livestock and poultry, the feed additive, the preparation method and the application thereof provided by the present invention are provided with the accompanying drawings and examples, but they should not be construed as limiting the scope of the present invention.

Preparation example

Pulverizing the above six Chinese herbal materials (herba Epimedii, semen Cuscutae, radix astragali, radix Angelicae sinensis, herba Leonuri and Glycyrrhrizae radix), and sieving with 80 mesh sieve.

The preparation examples all adopt the traditional Chinese medicine components which are crushed and sieved.

Example 1

Weighing 30g of epimedium herb, and treating the epimedium herb by using a microbial fermentation treatment method, wherein the specific condition parameters of the microbial fermentation treatment are as follows:

based on the mass of dry matter of epimedium, namely 30g, the addition amount of the microbial inoculum is 5 multiplied by 10 ⁷ CFU/g (mass ratio of Bacillus licheniformis powder to Bacillus coagulans powder to Aspergillus niger powder is 1; when in biological fermentation, the water content of the epimedium is 45 wt%;

crushing and drying epimedium herb obtained by biological fermentation treatment, wherein the grain diameter of astragalus mongholicus obtained after crushing is sieved by a 80-mesh sieve, and the drying temperature is 33-37 ℃; the standard of the drying is that the water content of the dried product obtained after drying reaches the initial water content, which is detailed in table 1.

Example 2

Weighing 30g of epimedium herb, and treating by using an enzymolysis treatment method, wherein the specific condition parameters of the enzymolysis treatment are as follows:

based on the mass of the epimedium with initial moisture, the addition amount of the complex enzyme preparation (each g of the complex enzyme preparation, the activity of xylanase is 8000u/g, the activity of cellulase is 30u/g, and the activity of laccase is 4000 u/g) is 5kg/t, the enzymolysis time is 7h, and the enzymolysis temperature is 55-60 ℃; during enzymolysis, the water content of epimedium is 45 wt%;

pulverizing herba Epimedii obtained by enzymolysis, and oven drying, wherein the particle diameter of radix astragali obtained after pulverizing is 80 mesh, and oven drying temperature is 55 deg.C; the standard of the drying is that the water content of the dried product obtained after the drying reaches the initial water content, which is detailed in table 1.

Example 3

Weighing 30g of epimedium, and treating by using a bacterial enzyme synergistic fermentation treatment method, wherein the specific condition parameters of the bacterial enzyme synergistic fermentation treatment are as follows:

based on the mass of the epimedium with initial moisture, the addition amount of the microbial inoculum is 5 multiplied by 10 ⁷ CFU/g (adding proportion of bacillus licheniformis, bacillus coagulans and aspergillus niger is 1;

pulverizing and drying epimedium obtained by bacterial enzyme synergistic fermentation treatment, wherein the particle size of the epimedium obtained after pulverization is sieved by a 120-mesh sieve, and the drying temperature is 33-37 ℃; the standard of the drying is that the water content of the dried product obtained after drying reaches the initial water content, which is detailed in table 1.

Comparative example 1

30g of epimedium herb is weighed out without any treatment.

Example 4

The only difference was that the ingredient was angelica sinensis, which was the same procedure as in example 1.

Example 5

The only difference is that the ingredient is angelica sinensis, which is the same as the step of the example 2.

Example 6

The procedure of example 3 was followed, except that the ingredient was Angelica sinensis.

Comparative example 2

30g of Chinese angelica was weighed out without any treatment.

Example 7

The same procedure as in example 1 was repeated except that the ingredient was licorice.

Example 8

The same procedure as in example 2 was followed, except that the ingredient was licorice.

Example 9

The same procedure as in example 3 was repeated except that the ingredient was licorice.

Comparative example 3

30g of licorice was weighed out without any treatment.

Example 10

The only difference is that the component is dodder seed, which is the same as the step of the example 2.

Comparative example 4

The same procedure as in example 1 was followed, except that the ingredient was dodder.

Comparative example 5

The same procedure as in example 3 was followed, except that the ingredient was dodder.

Comparative example 6

30g of dodder seed was weighed out without any treatment.

Example 11

The only difference is that the ingredient is astragalus root, which is the same as the step of the example 2.

Comparative example 7

The only difference is that the ingredient is astragalus root, which is the same as the step of the example 1.

Comparative example 8

The only difference is that the ingredient is astragalus root, which is the same as the step of the embodiment 3.

Comparative example 9

30g of Astragalus membranaceus was weighed out without any treatment.

Example 12

30g of motherwort was weighed out without any treatment.

Example 13

The only difference is that the component is motherwort as the same procedure as the step of the example 1.

Comparative example 10

The only difference is that the component is motherwort as the same step as the step of the example 2.

Comparative example 11

The only difference is that the component is motherwort as in the step of the embodiment 3.

Extracting the processed medicinal materials and the unprocessed medicinal materials respectively and independently, and selecting an optimal processing method by measuring different indexes, wherein the content of flavone is measured by using epimedium and semen cuscutae; determining polysaccharide content of radix Angelicae sinensis, radix astragali and Glycyrrhrizae radix; herba Leonuri measures leonurine content.

TABLE 1 initial moisture content of different herbs

Determination of medicinal materials	Water content/wt. -%)
		Herba Epimedii	9.92
Semen Cuscutae	8.70
		Radix Angelicae sinensis	10.47
Astragalus membranaceus	9.15
		Motherwort herb	9.49
Licorice root, radix Glycyrrhizae	9.08

Extraction and determination method of Chinese herbal medicine

The extraction and determination method of polysaccharide comprises the following steps:

the extraction method of the polysaccharide comprises the following steps: accurately weighing 5g of a single traditional Chinese medicine, placing the single traditional Chinese medicine into a 250mL conical flask, adding 40mL of distilled water (material-liquid ratio is 1.

Filtering the heated medicine with gauze, centrifuging for 10min under the condition of 3500r/min, taking supernatant, putting the supernatant into a rotary evaporation concentrator, concentrating the volume of the solution to 10mL, adding ethanol with the volume percentage content of 95% which is 4-6 times of the volume of the solution, shaking, standing, precipitating after a moment, putting the solution into a centrifugal tube, centrifuging for 10min at 3500r/min, discarding the supernatant, and taking precipitate. And (3) putting the precipitate into a drying oven at 65 ℃ for drying for 1.5h to obtain crude polysaccharide.

Preparation of polysaccharide standard curve:

(1) preparing a phenol solution: accurately weighing 50g of phenol, adding a proper amount of water to dissolve the phenol, fixing the volume to a 100mL volumetric flask to obtain a phenol solution with the mass percentage of 50% (the solution can be stored for a long time), sucking 5mL of the phenol solution with the mass percentage of 50%, fixing the volume to the 50mL volumetric flask to obtain a phenol solution with the mass percentage of 5% (the solution is prepared for use);

(2) preparing a glucose standard solution: accurately weighing 200mg of glucose, and fixing the volume to a 100mL volumetric flask to obtain a solution with the concentration of 2 mg/mL; taking 2mL of solution with the concentration of 2mg/mL, adding distilled water to a constant volume of 50mL of volumetric flask to obtain 0.08mg/mL of glucose standard solution;

(3) preparation of a standard curve: sucking 0mL, 0.2mL, 0.4mL, 0.6mL, 0.8mL, 1.0mL, 1.2mL of the standard solution prepared in the step (2), adding distilled water to 2mL, and shaking up; then adding 1mL of phenol solution with the mass percentage content of 5% and shaking up, adding 5mL of concentrated sulfuric acid and shaking up, standing in a greenhouse for 10min, carrying out boiling water bath for 15min, and cooling to room temperature in an ice bath. Measuring absorbance at 490nm wavelength with ultraviolet spectrophotometer, with polysaccharide concentration as abscissa and absorbance value as ordinate, obtaining linear regression equation of y =9.95x +0.0161, R ² =0.998, with a good linear relationship, standard graph as shown in figure 1.

And (3) determining the polysaccharide content: crushing the obtained sample, dissolving the crushed sample with distilled water, fixing the volume of the solution in a volumetric flask with 50mL, extracting 5mL of the solution, fixing the volume of the solution in a volumetric flask with 100mL of the solution with distilled water, sucking 0.8mL of the solution, measuring the light absorption value according to the operation of the step (3), and calculating the value according to a formula M = (CV/M) × 100%, wherein C: determination of sample concentration, V: measurement of sample volume, m: the quality of the sample to be tested.

The extraction and determination method of flavone comprises the following steps: accurately weighing 5g of the medicine, placing the medicine in a round-bottom flask, adding 75mL (material-liquid ratio is 1: 15) of ethanol with the ethanol concentration of 70% (v/v), and heating in a water bath for 100min at 70 ℃. The heated solution is filtered and is measured in a volumetric flask which is constant-volume to 100mL with ethanol of the same concentration.

Preparation of a standard curve: (1) preparation of standard solution: taking 25mg of rutin reference substance, placing in a50 mL volumetric flask, adding a small amount of 95% ethanol by volume, dissolving, diluting to 50mL, and shaking up to obtain a rutin reference substance solution (0.5 mg/mL); (2) preparation of a standard curve: precisely absorbing 0.2mL, 0.4mL, 0.6mL, 0.8mL, 1.0mL and 1.2mL of rutin control solution respectively, placing the rutin control solution in a 10mL test tube with a plug, adding 5.0mL of water respectively, then adding 0.6mL of 5% sodium nitrite solution by mass percent, shaking up, placing for 6min, then adding 0.6mL of 10% aluminum nitrate solution by mass percent, shaking up, placing for 6min, then adding 3.0mL of 4% sodium hydroxide solution by mass percent, adding water to 10mL, shaking up, placing for 15min, and measuring the absorbance at the wavelength of 506nm by using an ultraviolet spectrophotometer;

taking the concentration of the standard substance as an abscissa and the light absorption value as an ordinate, drawing a standard curve, obtaining a linear regression equation of y =9.6929x +0.0168, R ² =0.9804, with a good linear relationship, the standard curve after plotting is shown in fig. 2.

The extraction and determination method of leonurine comprises the following steps:

the extraction method of leonurine comprises the following steps: accurately weighing 10.0g of single Chinese medicinal material, adding 100mL of glacial acetic acid with volume percentage of 5% into a 250mL volumetric flask, placing in a water bath at 60 deg.C, and extracting leonurine under magnetic stirring (500 rpm) for 120min.

The method for determining leonurine comprises the following steps: precisely weighing 25mg of leonurine standard substance, and adding methanol to prepare 1mL of solution containing 0.1mg as a reference substance solution; accurately weighing 1.0g of extraction mother liquor, adding 50mL of methanol containing 5% phosphoric acid by volume percentage, carrying out ultrasonic treatment for 30min, cooling and filtering, transferring the filtrate to a 100mL volumetric flask, adding methanol to 100mL, shaking up, and filtering the filtrate by using a 0.22 mu m microporous filter to obtain a sample to be detected;

selecting a sample to be detected as C ₁₈ And (3) a chromatographic column, wherein the mobile phase is 10mmol/L phosphoric acid-ammonium dihydrogen phosphate (pH = 3.5) methanol solution, the flow rate is 0.3mL/min, the detection wavelength is 254nm, the column temperature is 30 ℃, the sample injection amount is 20 mu L, and the leonurine content in the extraction mother liquor under different conditions is obtained by an external standard method.

Data analysis

The test data were processed through Excel and subjected to one-way ANOVA in SPSS17.0 software for one-way analysis of variance. Data were tested for homogeneity of variance (Levene's) prior to analysis. Multiple comparisons were performed using the Duncan method. The difference was not significant as (P > 0.05), (P < 0.05) significant, and (P < 0.01) very significant. Data results are expressed as "mean ± standard deviation".

The influence of different treatment methods on the content of epimedium flavone is shown in table 2.

TABLE 2 Epimedium flavone content table

Processing method	Untreated with	Enzymolysis	Microbial fermentation	Bacterial enzyme synergistic fermentation
					Flavone content (mg/g)	19.37±0.89 ^d	21.23±0.59 ^c	25.24±0.56 ^a	23.37±0.42 ^b

Note: the capital different letters of the same row showed extremely significant differences (P < 0.01) and the same letters showed insignificant differences (P > 0.05).

As can be seen from Table 2, the content of epimedium flavone treated by different treatment methods is remarkably higher than that of untreated epimedium (P is less than 0.01), wherein the content of epimedium flavone after microbial fermentation is the highest and is 30.30 percent higher than that of untreated epimedium (P is less than 0.01); secondly, the content of epimedium flavone after the fungus enzyme synergistic fermentation is improved by 20.65 percent (P is less than 0.01) compared with that of the epimedium flavone which is not treated; finally, the content of epimedium flavone after enzymolysis treatment is 9.60 percent higher than that of the epimedium flavone without treatment (P is less than 0.01).

The effect of different treatments on the content of angelicae polysaccharide is shown in table 3.

TABLE 3 Angelica polysaccharide content

Processing method	Untreated	Enzymolysis	Microbial fermentation	Bacterial enzyme synergistic fermentation
					Polysaccharide content mg/g	12.38±0.32 ^d	21.86±0.24 ^c	53.80±0.44 ^b	58.43±1.29 ^a

As can be seen from Table 3, the treated and untreated ones were very different (P < 0.01) and the content of Angelica sinensis polysaccharide was higher than that of the untreated one. The difference of the bacterial enzyme synergistic fermentation treatment, the enzymolysis treatment and the microbial fermentation treatment is very obvious (P is less than 0.01), the content of the angelica polysaccharide is 58.43mg/g at most, and is 372 percent higher than that of the angelica polysaccharide which is not treated (P is less than 0.01).

The effect of different treatments on the glycyrrhiza polysaccharide content is shown in table 4.

TABLE 4 Glycyrrhiza polysaccharide content scale

Processing method	Untreated	Enzymolysis	Microbial fermentation	Bacterial enzyme synergistic fermentation
					Polysaccharide content mg/g	10.77±0.24 ^b	15.08±0.68 ^a	11.17±0.58 ^b	14.21±0.43 ^a

As can be seen from Table 4, the content of glycyrrhiza polysaccharide in the enzymolysis treatment and the bacterial enzyme synergistic fermentation treatment is significantly higher than that in the untreated treatment and the microbial fermentation (P is less than 0.01), the difference between the untreated treatment and the microbial fermentation treatment is not significant (P is more than 0.05), but the difference between the microbial fermentation treatment and the enzymolysis treatment and the bacterial enzyme synergistic fermentation treatment is not significant (P is more than 0.05).

The effect of different treatment methods on the content of flavonoids in semen cuscutae is shown in table 5.

TABLE 5 semen Cuscutae flavone content

Processing method	Untreated with	Enzymolysis	Microbial fermentation	Bacterial enzyme synergistic fermentation
					Flavone content (mg/g)	9.06±0.31 ^b	13.72±0.47 ^a	5.59±0.17 ^c	5.48±0.48 ^c

Note: the capital different letters of the same row showed significant differences (P < 0.01), and the same letters showed insignificant differences (P > 0.05).

As can be seen from Table 5, the influence of different treatment methods on the content of flavonoids in semen Cuscutae is very significant (P is less than 0.01), wherein the flavonoids in semen Cuscutae treated by enzymolysis have the highest content, which is significantly higher than that of the other three groups (P is less than 0.01), and is 51.43% higher than that of the semen Cuscutae not treated; after the dodder is subjected to microbial fermentation treatment and bacterium enzyme synergistic fermentation treatment, the flavone content is remarkably reduced compared with that of the dodder which is not treated.

The effect of different treatments on the astragalus polysaccharide content is shown in table 6.

TABLE 6 Astragalus polysaccharides table

Processing method	Untreated with	Enzymolysis	Microbial fermentation	Bacterial enzyme synergistic fermentation
					Polysaccharide content mg/g	24.47±0.57 ^b	43.97±0.44 ^a	8.12±0.34 ^c	6.37±0.09 ^d

As can be seen from Table 6, the difference of Astragalus polysaccharides (P < 0.01) is very significant when the enzymolysis treatment is compared with the untreated treatment, and the content of the untreated Astragalus polysaccharides is significantly higher than that of the microbial fermentation treatment and the bacterial enzyme synergistic fermentation treatment (P < 0.01); compared with the microbial fermentation treatment and the bacterial enzyme synergistic fermentation treatment, the difference is very obvious (P is less than 0.01); the content of enzymolysis treatment is the highest, and is 43.97mg/g, which is 79.69 percent higher than that of untreated treatment.

The effect of different treatments on leonurine content, the results are shown in table 7.

TABLE 7 content of leonurine

Processing method	Untreated	Enzymolysis	Microbial fermentation	Bacterial enzyme synergistic fermentation
					Leonurine mg/g	0.57±0.06 ^a	0.54±0.06 ^a	0.57±0.02 ^a	0.53±0.08 ^a

Note: the lower case different letters of the same row indicate significant difference (P < 0.01), and the same letters indicate insignificant difference (P > 0.05).

As can be seen from Table 7, the difference in the leonurine content was not significant (P > 0.05) between the untreated group and the other groups, but the values were higher for both the untreated group and the group treated by microbial fermentation.

According to the data of the embodiment and the comparative example, the six-ingredient estrus-promoting pregnancy traditional Chinese medicine components are respectively subjected to in-vitro pretreatment by three methods of microbial fermentation treatment, enzymolysis treatment and bacterial enzyme synergistic fermentation treatment, the optimal treatment scheme of each traditional Chinese medicine is selected according to the content of the main components measured in each traditional Chinese medicine, and the optimal in-vitro pretreatment method of the epimedium herb is microbial fermentation treatment; the optimal in-vitro pretreatment method of the dodder, the liquorice and the astragalus root is enzymolysis treatment; the optimal treatment method of the angelica is the bacterium enzyme synergistic fermentation treatment; herba Leonuri should not be processed.

Example 14

Weighing 20g of herba Epimedii, 20g of semen Cuscutae, 20g of radix astragali, 40g of radix Angelicae sinensis, 20g of herba Leonuri and 10g of Glycyrrhrizae radix.

Wherein epimedium is treated by the method of example 1, dodder is treated by the method of example 2, astragalus is treated by the method of example 2, angelica is treated by the method of example 3, leonurus is not treated at all, and licorice is treated by the method of example 2;

mixing the above processed materials to obtain the Chinese medicinal composition.

Example 14-2

A Chinese medicinal composition was prepared in the same manner as in example 14, except that motherwort was subjected to microbial fermentation treatment.

Examples 14 to 3

A Chinese medicinal composition was prepared in the same manner as in example 14, except that epimedium was subjected to microbial fermentation treatment.

Examples 14 to 4

A Chinese medicinal composition was prepared in the same manner as in example 14, except that epimedium was subjected to a fungus-enzyme synergistic fermentation treatment.

Examples 14 to 5

A Chinese medicinal composition was prepared in the same manner as in example 14, except that angelicae gigantis radix was subjected to microbial fermentation treatment.

Examples 14 to 6

A Chinese medicinal composition was prepared in the same manner as in example 14, except that angelicae gigantis radix was subjected to fungal enzyme co-fermentation treatment.

Examples 14 to 7

A Chinese medicinal composition was prepared in the same manner as in example 14, except that licorice was subjected to microbial fermentation treatment.

Examples 14 to 8

A Chinese medicinal composition was prepared in the same manner as in example 14, except that licorice was subjected to a fungal enzyme co-fermentation treatment.

Application example 1

The test adopts a single-factor completely random test design, 180 binary hybrid backup sows with similar age in days (150 +/-5 days) and weight (120 +/-10 kg), health, no disease and good growth and body condition are selected and randomly divided into 3 groups, the groups are marked as a control group, a treatment group and an untreated group, each treatment group has 6 repetitions, and each repetition has 10 pigs. The transition period was 3 days and the official test was 44 days. The Chinese medicinal composition prepared in example 14 is added to each pig by 50g per day, and feeding is stopped after the sows are in estrus. The experimental diets were grouped as shown in table 8, and the control group was given a basal diet; the untreated group diet was 1.5kg of basal diet plus 50g of untreated traditional Chinese medicine composition (herba epimedii, semen cuscutae, radix astragali, angelica sinensis, leonurus japonicus and liquorice were respectively pulverized and sieved through a 80-mesh sieve, and the obtained components were mixed according to the amount in example 14); the treatment group had a daily ration of 1.5kg basal daily ration plus 50g of the traditional Chinese medicine composition prepared in example 14, the specific method was:

the test was carried out by Shenyang Borui farming science and technology Co., ltd, and the breeding management strictly followed the procedure of the farm. The pigsty is cleaned and disinfected before the test, so that ventilation and good environment are ensured. The replacement gilt is transferred into a breeding house and transited for 3 days, the feeding condition and the body condition of the gilt are observed, the feeding is limited, each gilt is fed once at 7 am and 1 pm every day, 3kg in total, and the gilt is freely drunk. 1.5kg of basal diet and 50g of the Chinese medicinal composition prepared in example 14 were fed every morning and 1.5kg of basal diet was fed every afternoon, and feeding was stopped after the sows estrus. And (5) entering a formal test after the transition period is finished, and continuously feeding for 14 days. During the test period, the situation is checked once every morning and afternoon, and the situation checking record is made seriously. When the sows have standing reflex, red and swollen vulva and mucus, first hybridization is started, compounding is carried out the next day, the time for two hybridizations does not exceed 24 hours, and hybridization records are made. Sperm motility was checked before each mating, and the mating work was done by a professional researcher in the factory. The hybridization time of each pig is recorded, after 30 days, B-ultrasonic diagnosis is carried out, and whether pregnancy occurs or not is checked and well recorded.

TABLE 8 basal daily ration table for sow

Note: 1) The compound premix provides 100mg/kg of Fe, 5mg/kg of Cu, 50mg/kg of Zn, 20mg of Mn, 0.24mg/kg of I, 0.24mg/kg of Se, 5000 IU/kg of VA, 900IU/kg of VD, 40IU/kg of VE, 2.5mg/kg of VK, 6.4mg/kg of riboflavin, 22mg/kg of nicotinic acid, 22mg/kg of D-pantothenic acid, 1225mg/kg of VB and 0.36mg/kg of biotin for each kilogram of feed;

2) The nutrient levels are calculated values.

Index measurement

Oestrus index: the estrus index of replacement gilts: (1) oestrus rate in 7 days: the number of sows in heat in 7 days of the test accounts for the percentage of the total number of sows; (2) oestrus rate in 14 days: the number of sows in estrus in 14 days of the test accounts for the percentage of the total number of sows; (3) pregnancy rate: percentage of pregnant sows in total;

serum hormone index:

blood sample collection: the replacement gilts take anterior vena cava blood sampling after one sow is repeatedly taken out and two hours of standing reflex in each group on the seventh day and the fifth day of the test respectively, and each sow takes 5-10 mL. Standing the blood sample for half an hour, centrifuging at 4000r/min for 10min, and storing the separated serum in a centrifuge tube at-80 deg.C.

Hormone determination: four hormones (progesterone, estradiol, luteinizing hormone and follicle stimulating hormone) are measured by using an enzyme-linked immunosorbent assay, and all used kits are purchased from Shanghai enzyme-linked biotechnology limited company and specifically comprise: a progestogen PROG ELISA kit, an estrogen E2 ELISA kit, a luteinizing hormone LH ELISA kit and a follicle stimulating hormone FSH ELISA kit, and the determination procedure is carried out according to the kit instruction.

Data analysis

After the data are preliminarily arranged by using an Excel table, statistical analysis is carried out by using a single-factor analysis of variance (ANOVA) method in SPSS17.0 software, and significance of the mean value is tested by using a Duncan method. The judgment standard is that P <0.05 represents significant difference, P <0.01 represents significant difference, and the data result is represented by 'mean plus minus standard deviation'.

Results and analysis

The influence of the traditional Chinese medicine composition prepared in example 14 on the oestrus rate and pregnancy rate in the reproductive performance of replacement gilts is shown in table 9.

Table 9 influence of the herbal composition prepared in example 14 on the oestrus rate index of replacement gilts

Index (es)	Control group	Untreated group	Treatment group
				7 days estrus (%)	40.00±6.33 ^B	43.33±5.16 ^B	51.67±4.08 ^A
14 days estrus (%)	51.67±4.08 ^C	63.33±5.16 ^B	80.00±6.33 ^A
				Pregnancy Rate (%)	50.00±6.33 ^B	51.67±4.08 ^B	65.00±5.48 ^A

Note: the same row has marked difference in different lower case letters (P < 0.05) and the same row has marked difference in different upper case letters (P < 0.01) and has marked difference in the same letter (P > 0.05), and the following table is the same.

As can be seen from Table 9, the data of the 7-day estrus rate in the treated group was significantly higher than those in the other two groups (P < 0.01), and the treated group was 11.67% higher than the control group and 8.34% higher than the untreated group. The estrus rate of the untreated group in 7 days is not significantly different from that of the control group (P is more than 0.05). The 14-day oestrus rate of the treated group is remarkably higher than that of the other two groups (P is less than 0.01), and the treated group is 28.33 percent higher than that of the control group and 16.67 percent higher than that of the untreated group. The oestrus rate of the untreated group is remarkably higher than that of the control group (P is less than 0.01) and is 11.66 percent higher than that of the control group. The pregnancy rate of the treated group is obviously higher than that of the other two groups (P is less than 0.05), and the pregnancy rate of the treated group is 13.33 percent higher than that of the untreated group and is 15 percent higher than that of the control group. The pregnancy rate of the untreated group was not significantly different from the control group (P < 0.05).

The influence of the traditional Chinese medicine composition prepared in example 14 on the hormone index content of replacement gilts is shown in table 10.

Table 10 effect of the herbal composition prepared in example 14 on the hormone index levels in replacement gilts

Note: the shoulder notes of the same row with different lower case letters show significant difference (P < 0.05), the shoulder notes of the same row with different upper case letters show significant difference (P < 0.01), the shoulder notes with the same letter show insignificant difference (P > 0.05), and the following table shows the same.

As can be seen from Table 10, the difference between the progestogen content of the treated group and the control group was not significant (P > 0.05), and the difference between the control group and the untreated group was not significant (P > 0.05). The treated group differed significantly (P > 0.05) compared to the untreated group. The estrogen content of the treated group is very higher than that of the other two groups (P is less than 0.01), and the difference of the untreated group and the control group is not significant (P is more than 0.05). The treated group had no significant difference in the content of luteinizing hormone (P > 0.05) compared to the untreated group, but all were significantly higher than the control group (P < 0.01). The content of the follicle-stimulating hormone in the treated group is remarkably higher than that in the control group (P is less than 0.01), the difference is not remarkable compared with the untreated group (P is more than 0.05), and the difference is not remarkable compared with the control group (P is more than 0.05).

Application example 2

90 healthy and disease-free multiparous sows with the same number of births (2-3 births), similar mating date, no obvious difference in reproductive performance and body condition are randomly divided into 3 treatment groups, each treatment group has 6 repetitions, and each repetition has 5 pigs. The second day after the sow is weaned, the test period is 37 days, and the test diet and the group are completely consistent with the group of replacement gilts in the application example 1.

The multiparous sows are transferred to a breeding house on the weaning day, the test formally starts on the next day, and the feeding method is consistent with that of the replacement sows in the application example 1. During the test period, the situation is checked once every morning and afternoon, and the situation checking records are made carefully. When the sows have standing reflex, red and swollen vulva and mucus, first hybridization is started, compounding is carried out the next day, the time for two hybridizations does not exceed 24 hours, and hybridization records are made. Sperm motility was checked before each mating, and the mating work was done by a professional researcher in the factory. The hybridization time of each pig is recorded, and after 30 days, B-ultrasonic diagnosis is carried out, and whether pregnancy occurs or not is checked and recorded.

Index measurement

Oestrus indexes of multiparous sows: (1) oestrus rate in 5 days: the number of the sows in oestrus for 5 days in the test accounts for the percentage of the total number; (2) oestrus rate in 7 days: the number of sows in heat in 7 days of the test accounts for the percentage of the total number of sows; (3) pregnancy rate: percentage of pregnant sows in total; (4) estrus interval: the time interval from weaning to estrus;

serum hormone index:

blood sample collection: the multiparous sows are respectively tested on the seventh day and the fifth day, one sow is repeatedly taken out from each group, the anterior vena cava blood sampling is carried out after two hours of standing reflex, and each sow takes 5-10 mL. Standing the blood sample for half an hour, centrifuging at 4000r/min for 10min, and storing the separated serum in a centrifuge tube at-80 deg.C.

Hormone determination: four hormones (progesterone, estradiol, luteinizing hormone and follicle stimulating hormone) are determined by an enzyme linked immunosorbent assay, all the kits are purchased from Jiangsu Baolai enzyme immunoassay biotech GmbH, and the determination procedure is carried out according to the kit instruction.

Data analysis

After the data are preliminarily arranged by using an Excel table, statistical analysis is carried out by using a single-factor analysis of variance (ANOVA) method in SPSS17.0 software, and significance of the mean value is tested by using a Duncan method. The judgment standard is that P <0.05 indicates significant difference, P <0.01 indicates significant difference, and the data result is expressed as 'mean. + -. Standard deviation'.

Results and analysis

The influence of the Chinese medicinal composition prepared in example 14 on oestrus rate and pregnancy rate in reproductive performance of multiparous sows is shown in table 11.

TABLE 11 influence of the Chinese medicinal composition prepared in example 14 on oestrus rate index of multiparous sow

Index (I)	Control group	Untreated group	Treatment group
				5-day estrus (%)	40±0.00 ^C	56.67±8.17 ^B	70±10.95 ^A
7 days estrus (%)	86.67±10.33 ^a	90±10.95 ^a	96.67±8.17 ^a
				Estrus interval (sky)	6.08±0.90 ^a	5.75±0.75 ^ab	5.00±1.13 ^b
Pregnancy Rate (%)	73.33±10.33 ^a	76.76±8.17 ^a	83.33±8.17 ^a

As can be seen from Table 11, comparing the oestrus levels for 5 days, the treated group was significantly higher than the other two groups (P < 0.01), 30% and 13.33% higher than the control group and the untreated group, respectively; the untreated group was significantly higher than the control group (P < 0.01), 16.67% higher than the control group.

Comparing the estrus level for 7 days, the treated group was higher than the other two groups, but the difference was not significant (P > 0.05), which was 10% higher and 6.67% higher than the control group and the untreated group, respectively; the untreated group was higher than the control group with no significant difference (P > 0.05) and 3.33% higher than the control group. The estrus interval of the treated group is significantly lower than that of the control group (P < 0.05), and the difference is not significant compared with that of the untreated group (P > 0.05). The estrus interval difference was not significant (P > 0.05) in the untreated group compared to the control group. The pregnancy rate of the treated group was 10% and 6.57% higher than those of the control group and the untreated group, respectively, but the difference was not significant (P > 0.05), and the pregnancy rate of the untreated group was 3.33% higher than that of the control group and the difference was not significant (P > 0.05).

The influence of the traditional Chinese medicine composition prepared in example 14 on the hormone index content of the multiparous sows is shown in table 12.

TABLE 12 influence of the herbal composition prepared in example 14 on the hormone levels in gilts

As can be seen from Table 12, the progestogen content in the treated group was significantly higher than that in the other two groups (P < 0.01), and the difference between the untreated group and the control group was not significant (P > 0.05). The estrogen content of the treated group is very higher than that of the other two groups (P is less than 0.01), and the difference of the untreated group and the control group is not significant (P is more than 0.05). The content of the luteinizing hormone in the treated group and the untreated group is remarkably higher than that in the control group (P < 0.01), and the difference between the treated group and the untreated group is remarkable (P < 0.05). The content of the follitropin in the treated group is remarkably higher than that in the other two groups (P is less than 0.01), and the content of the follitropin in the untreated group is remarkably higher than that in the control group (P is less than 0.01).

According to the records, the traditional Chinese medicine composition subjected to in-vitro pretreatment is added into the basal ration of the replacement gilt, so that the oestrus rate and pregnancy rate of the replacement gilt can be obviously improved, and the contents of progestogen, estrogen, luteinizing hormone and follicle stimulating hormone can be obviously improved. The traditional Chinese medicine composition after in-vitro pretreatment is added into the basic daily ration of the multiparous sow, so that the oestrus rate and the pregnancy rate of the multiparous sow can be improved, the oestrus interval is obviously shortened, and the contents of progestogen, estrogen, luteinizing hormone and follicle stimulating hormone are obviously improved.

The optimal treatment method of the epimedium in the traditional Chinese medicine composition is microbial fermentation, the optimal treatment method of the dodder, the astragalus and the liquorice is enzymolysis, the optimal treatment method of the angelica is bacterial enzyme synergistic fermentation, and the motherwort is not treated. The traditional Chinese medicine composition after in vitro pretreatment can obviously improve the oestrus rate and pregnancy rate of replacement gilts, improve the oestrus rate and pregnancy rate of multiparous sows and obviously shorten oestrus intervals. And can obviously improve the contents of progestogen, estrogen, luteinizing hormone and follicle stimulating hormone of replacement sows and multiparous sows.

In conclusion, the Chinese herbal medicines can improve the content of effective active ingredients of the Chinese herbal medicines after being treated, and improve the utilization rate of the Chinese herbal medicine composition. The compound Chinese herbal medicine is added into the daily ration of the sow, so that the oestrus rate and pregnancy rate of the sow can be improved, and the reproductive performance of the sow can be improved.

Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.

Claims

1. A traditional Chinese medicine composition for promoting oestrus of livestock and poultry is characterized by comprising the following components in parts by mass:

2. The preparation method of the traditional Chinese medicine composition as claimed in claim 1, which is characterized by comprising the following steps:

3. The preparation method according to claim 2, wherein the dodder is an enzymatically treated product; the astragalus is a product after enzymolysis; the epimedium is a product subjected to enzymolysis treatment, microbial fermentation treatment or bacterium-enzyme synergistic fermentation treatment; the angelica is a product subjected to enzymolysis treatment, microbial fermentation treatment or bacterial enzyme synergistic fermentation treatment; the liquorice is a product obtained by enzymolysis, microbial fermentation or bacterial enzyme synergistic fermentation; the herba Leonuri is untreated herba Leonuri or product obtained by fermenting with microorganism.

4. The preparation method according to claim 3, wherein when the epimedium herb is a product subjected to enzymolysis, the licorice root is a product subjected to enzymolysis, and the leonurus is an untreated leonurus.

5. The preparation method according to claim 3 or 4, characterized in that the enzyme preparation used in the enzymolysis treatment is a complex enzyme preparation, and the complex enzyme preparation comprises xylanase, cellulase and laccase; the activity of the xylanase is 8000u/g, the activity of the cellulase is 30u/g, and the activity of the laccase is 4000u/g; the enzymolysis treatment time is 6-12 h, and the temperature is 50-60 ℃.

6. The preparation method according to claim 3 or 4, wherein the microbial fermentation treatment is carried out by using microbial agents including Bacillus licheniformis, bacillus coagulans and Aspergillus niger; the time of the microbial fermentation is 3-5 days, and the temperature is 30-40 ℃.

7. The preparation method according to claim 3 or 4, characterized in that when the bacterial enzymes are subjected to synergistic fermentation treatment, the enzyme preparation adopted is a complex enzyme preparation, and the complex enzyme preparation comprises xylanase, cellulase and laccase; the activity of the xylanase is 8000u/g, the activity of the cellulase is 30u/g, and the activity of the laccase is 4000u/g; when the bacterial enzymes are used for fermentation in a synergistic manner, the adopted microbial inoculum comprises bacillus licheniformis, bacillus coagulans and aspergillus niger; the time of the synergistic fermentation of the bacterial enzymes is 3-5 days, and the temperature is 30-40 ℃.

8. A feed additive for promoting the oestrus of livestock and poultry, which is characterized in that the effective components of the feed additive comprise the traditional Chinese medicine composition of claim 1 or the traditional Chinese medicine composition prepared by the preparation method of any one of claims 2 to 7.

9. The use of the Chinese medicinal composition of claim 1, the Chinese medicinal composition obtained by the preparation method of any one of claims 2 to 7, or the feed additive of claim 8 in any one or more of improving the estrus rate of livestock and poultry, improving the pregnancy rate of livestock and poultry, shortening the estrus interval, and improving the reproductive performance.