CN115452507A - Array type biochip sample application device - Google Patents
Array type biochip sample application device Download PDFInfo
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- CN115452507A CN115452507A CN202211066755.2A CN202211066755A CN115452507A CN 115452507 A CN115452507 A CN 115452507A CN 202211066755 A CN202211066755 A CN 202211066755A CN 115452507 A CN115452507 A CN 115452507A
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- 238000000018 DNA microarray Methods 0.000 title claims abstract description 19
- 239000007921 spray Substances 0.000 claims abstract description 68
- 238000001125 extrusion Methods 0.000 claims abstract description 46
- 238000004080 punching Methods 0.000 claims abstract description 43
- 239000012528 membrane Substances 0.000 claims abstract description 38
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 239000000741 silica gel Substances 0.000 claims abstract description 24
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 24
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000003860 storage Methods 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 15
- 230000004907 flux Effects 0.000 claims abstract description 6
- 239000012472 biological sample Substances 0.000 claims description 9
- 230000000452 restraining effect Effects 0.000 claims description 9
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims 1
- 238000002347 injection Methods 0.000 abstract description 5
- 239000007924 injection Substances 0.000 abstract description 5
- 238000004140 cleaning Methods 0.000 abstract description 2
- 238000001035 drying Methods 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 32
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000002493 microarray Methods 0.000 description 3
- 229920001296 polysiloxane Polymers 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 238000003491 array Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses an array type biochip sample application device, which comprises a spray head, an array spray orifice assembly arranged in the spray head and a power assembly arranged above the spray head and used for applying downward impact force to the array spray orifice assembly; the array spray hole assembly comprises an array spray hole module, a silica gel membrane, a membrane fastening plate, an array punching needle and the like; the array spray hole module comprises a plurality of extrusion cavities which are arranged in an array, and the spray holes are positioned at the lower parts of the extrusion cavities, each spray hole in the sample application device provided by the invention is independently injected and independently sprayed, so that the pollution-free and high-precision requirements are met, different samples can be added into different liquid storage cavities in the spray head, the simultaneous injection sample application of multiple samples is realized, the time for cleaning, drying and sample application again in the traditional sample application process is reduced, and the preparation efficiency of a biochip is improved; can satisfy many orifices sample application simultaneously, satisfy high flux, modular sample application demand.
Description
Technical Field
The invention belongs to the technical field of biological instruments and equipment, and particularly relates to an array type biochip sample application device.
Background
The sample application head adopted by the sample application instrument in the market is mainly a contact sample application needle, the contact sample application has higher risk of cross contamination of reagents under the condition of repeated sample application, and the sample application needle is easy to damage and the preparation efficiency of a microarray chip is low. For non-contact sample application, the problems of contact sample application cross contamination, damage of a sample application needle touching a substrate and the like can be solved well independently in the sample application process. The existing non-contact type sample application spray head on the market has the problems of low sample application efficiency, long time consumption, complex device, high manufacturing cost, extremely inconvenient non-modularized assembly and disassembly and the like due to a series of processes of repeatedly cleaning the spray head, drying and the like when different biological samples are applied.
Disclosure of Invention
Aiming at the technical problems in the prior art, the invention aims to provide a sample application device of a microarray biochip, which has the advantages of simple structure, lower manufacturing cost, modularized assembly and disassembly and high sample application efficiency, and can realize large-area and high-density manufacturing of the microarray chip.
In order to achieve the purpose, the invention adopts the technical scheme that:
an array type biochip spotting device comprises a spray head, an array spray orifice assembly arranged in the spray head and a power assembly arranged above the spray head and used for applying downward impact force to the array spray orifice assembly; wherein:
the sprayer comprises a lower cover plate and an upper cover plate used for covering the lower cover plate, a plurality of first threaded holes are correspondingly formed in the peripheral sides of the lower cover plate and the upper cover plate, and the lower cover plate and the upper cover plate are fixedly connected through bolts penetrating through the first threaded holes; the middle part of the lower cover plate is provided with a first cavity for accommodating the array spray hole assembly, and the middle part of the first cavity is a through spray head through hole; the middle part of the upper cover plate is provided with a through guide hole; a force conduction positioning column is placed in the guide hole and is positioned right below the bottom of the power assembly, and the force conduction positioning column can avoid the phenomena of impact position deviation and uneven impact force when the power assembly directly impacts the array jet hole assembly; furthermore, a second cavity matched with the array punching needle in shape is formed in the middle of the bottom surface of the upper cover plate and used for limiting the position of the array punching needle and keeping the stability of the position of the array punching needle; the guiding hole is located the top middle part of second cavity and communicates with the second cavity.
The array spray hole assembly comprises an array spray hole module, a silica gel membrane, a membrane fastening plate and an array punching needle; the array spray hole module comprises a spray hole base block, the middle part of the spray hole base block is inwards sunken to form a spray hole cavity, the middle part of the spray hole cavity is provided with a plurality of extrusion cavities which are arranged in an array manner, and the middle part of each extrusion cavity is a spray hole which penetrates through the spray hole base block; furthermore, the number of the extrusion cavities in the array spray hole module is 1-48, the distance between two adjacent spray holes is 0.1-5 mm, the design of a plurality of spray holes can improve the sample application efficiency, and the sample application requirements of high flux and modularization are met. A plurality of liquid storage cavities for storing biological samples are arranged on the outer side of the extrusion cavity and on the periphery of the jet hole base block, the number of the liquid storage cavities is the same as that of the extrusion cavity, each liquid storage cavity is communicated with the extrusion cavity through a flow channel arranged in the jet hole base block, and sample introduction can be carried out between the liquid storage cavities and the extrusion cavity through capillary force; in order to prevent the biological sample from flowing back to the liquid storage cavity in the sample application process, one end of the flow channel, which is close to the extrusion cavity, is provided with a flow restraining structure, the flow restraining structure is in a boss shape, the flow restraining structure enables the liquid flux at the position, which is close to the liquid storage cavity, of the flow channel to be reduced, and liquid in the extrusion cavity is reduced from flowing back to the liquid storage cavity along the flow channel in the process that the punching needle head extrudes the silica gel membrane. The silica gel diaphragm is positioned right above the extrusion cavity and used for sealing the extrusion cavity; the array punching needle is positioned right above the silica gel membrane, the bottom of the array punching needle is provided with punching needle heads which are in one-to-one correspondence with the extrusion cavities, and the punching needle heads can extrude the silica gel membrane under the downward action generated by the power assembly, so that the biological sample in the extrusion cavities is ejected out from the jet holes in a liquid drop form, and the sample application is completed. The locating slot is arranged in the middle of the top surface of the array punching needle, the shape of the locating slot is matched with that of the bottom of the force conduction locating column, and the acting point of the force conduction locating column can be located in the middle of the array punching needle through the design of the locating slot, so that the locating precision is improved, and the stress uniformity of the array punching needle is guaranteed.
The membrane fastening plate is used for pressing the silica gel membrane to seal a squeezing cavity in the array spray hole module and is positioned between the array punching needle and the silica gel membrane; the middle part of the diaphragm fastening plate is provided with limiting holes which correspond to the punching heads one by one, and the punching heads are in clearance fit with the limiting holes and can penetrate through the limiting holes to extrude the silica gel diaphragm; the length of the punching head is 0.2-1 mm longer than the depth of the limiting hole. Furthermore, the longitudinal section of the membrane fastening plate is T-shaped and comprises a clamping part positioned at the upper part and a limiting part positioned at the lower part; the size of the limiting part is matched with that of the spray hole cavity; a clamping table is formed between the spray hole cavity and the spray hole base block, and the size of the clamping table is matched with the size of an area of the clamping portion, which exceeds the limiting portion.
Further, power component is electromagnetic power component, including installing electromagnet housing and the electromagnet core in the shower nozzle top, and the supporting seat that is used for fixed electromagnet housing is installed at the top of upper cover plate, and the second screw hole has been seted up along the horizontal direction to the lateral wall of supporting seat, and electromagnet housing is through wearing to establish the inside at the supporting seat at the bolt fastening in the second screw hole. When the electromagnet iron core moves downwards, the electromagnet iron core can apply downward acting force to the force conduction positioning column, and the array punching needle is synchronously driven to move in the downward movement process of the force conduction positioning column. In addition, the power assembly can be replaced by a piezoelectric column as a power source, and the piezoelectric column can generate downward impact force, so that the array spray hole assembly is extruded, and the sample application process is completed.
Furthermore, the array spray hole module, the membrane fastening plate, the array punching needle and the force conduction positioning column are processed by light curing of a photosensitive resin material, and a lower cover plate and an upper cover plate in the sprayer are processed and formed by laser of a PMMA material.
Compared with the prior art, the invention has the beneficial effects that:
the array spray hole module in the array spray hole assembly comprises a plurality of extrusion cavities which are arranged in an array manner, and the spray holes are positioned in the middle of the extrusion cavities, namely the spray holes are arranged in an array manner; can satisfy many orifices sample application simultaneously, satisfy high flux, modular sample application demand. In addition, in the device provided by the invention, the sample is injected between the liquid storage cavity and the extrusion cavity through capillary force, an external sample injection power source is not needed, the sample injection is stable, the device is small and exquisite, the design and the processing are simple, the manufacturing cost is low, and the integration and the scale are easy to realize.
Drawings
FIG. 1 is a schematic view of the entire structure of an array biochip spotting apparatus according to the present invention;
FIG. 2 is a cross-sectional view of FIG. 1;
FIG. 3 is a schematic illustration of the exploded structure of FIG. 1;
FIG. 4 is a schematic structural diagram of an array nozzle module;
reference numerals are as follows: 1-nozzle, 11-lower cover plate, 12-upper cover plate, 13-first threaded hole, 14-first cavity, 15-nozzle through hole, 16-guide hole, 17-second cavity, 21-array nozzle module, 211-nozzle base block, 212-nozzle cavity, 213-extrusion cavity, 214-nozzle, 215-liquid storage cavity, 216-flow channel, 217-flow inhibition structure, 22-silica gel membrane, 23-membrane fastening plate, 231-limiting hole, 232-clamping part, 233-limiting part, 24-array punching needle, 241-punching needle, 242-positioning groove, 25-clamping table, 3-power component, 31-electromagnet housing, 32-electromagnet core, 4-force conduction positioning column, 5-supporting seat, 6-second threaded hole, 7-biological sample.
Detailed Description
The present invention will be further described with reference to the following examples so that those skilled in the art can better understand the present invention and can practice the present invention, but the examples are not intended to limit the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus, are not to be construed as limiting the present invention. The terms "first" and "second" herein do not denote any particular quantity or order, but rather are used to distinguish one element from another.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "fixed," and the like are to be construed broadly and can include, for example, fixed connections, detachable connections, or integral connections; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Referring to fig. 1 to 4, an array type biochip spotting device includes a nozzle 1, an array nozzle assembly installed inside the nozzle 1, and a power assembly 3 installed above the nozzle 1 for applying a downward impact force to the array nozzle assembly; wherein:
the sprayer 1 comprises a lower cover plate 11 and an upper cover plate 12 for covering the lower cover plate, a plurality of first threaded holes 13 are correspondingly formed in the peripheral sides of the lower cover plate 11 and the upper cover plate 12, and the lower cover plate 11 and the upper cover plate 12 are fixedly connected through bolts penetrating through the first threaded holes 13; the middle part of the lower cover plate 11 is provided with a first cavity 14 for accommodating the array jet hole assembly, and the middle part of the first cavity 14 is a through nozzle through hole 15; the middle part of the upper cover plate 12 is provided with a through guide hole 16; a force conduction positioning column 4 is arranged in the guide hole 16, the force conduction positioning column 4 is positioned right below the bottom of the power assembly 3, and the force conduction positioning column 4 can avoid the phenomena of impact position deviation and uneven impact force when the power assembly 3 directly arrays the orifice assembly; furthermore, the middle part of the bottom surface of the upper cover plate 12 is provided with a second cavity 17 matched with the shape of the array punch pin 24, and the second cavity 17 is used for limiting the position of the array punch pin 24; the guide hole 16 is located at the upper middle portion of the second cavity 17 and communicates with the second cavity 17.
The array spray hole assembly comprises an array spray hole module 21, a silicon membrane 22, a membrane fastening plate 23 and an array punch needle 24; the array jet hole module 21 comprises a jet hole base block 211, the middle part of the jet hole base block 211 is recessed inwards to form a jet hole cavity 212, the middle part of the jet hole cavity 212 is provided with a plurality of extrusion cavities 213 which are arranged in an array manner, and the middle part of each extrusion cavity 213 is provided with jet holes 214 which penetrate through the jet hole base block; furthermore, the number of the extrusion cavities 213 in the array nozzle module is 1-48, and the distance between two adjacent nozzles 214 is 0.1-5 mm. A plurality of liquid storage cavities 215 for storing the biological samples 7 are arranged outside the extrusion cavity 213 and on the periphery of the orifice base block 211, the number of the liquid storage cavities 215 is the same as that of the extrusion cavity 213, and each liquid storage cavity 215 is respectively communicated with the extrusion cavity 213 through a flow channel 216 arranged in the orifice base block; in order to prevent the biological sample from flowing back to the liquid storage cavity 215 in the sample application process, the flow channel 216 is provided with a flow restraining structure 217 at one end close to the extrusion cavity 213, the flow restraining structure 217 is in a boss shape, and the flow restraining structure enables the liquid flux of the flow channel at the position close to the liquid storage cavity to be reduced. The silica gel membrane 22 is positioned right above the extrusion cavity 213 and used for sealing the extrusion cavity 213; the array punching needle 24 is positioned right above the silica gel membrane 22, the bottom of the array punching needle 24 is provided with punching needle heads 241 which are in one-to-one correspondence with the extrusion cavities 213, and the punching needle heads 241 can extrude the silica gel membrane under the downward action generated by the power assembly; the middle of the top surface of the array punch pin 24 is provided with a positioning groove 242, the shape of the positioning groove 242 is matched with the shape of the bottom of the force conduction positioning column 4, and the positioning groove 242 enables the acting point of the force conduction positioning column 4 to be positioned in the middle of the array punch pin 24, so that the stress uniformity of the array punch pin is increased.
The membrane fastening plate 23 is used for pressing the silicone membrane 22 to seal the extrusion cavity 213 inside the array orifice module, and is located between the array punch pin 24 and the silicone membrane 22; the middle part of the membrane fastening plate 23 is provided with limiting holes 231 corresponding to the punching needle heads 241 one by one, and the punching needle heads 241 are in clearance fit with the limiting holes 231 and can penetrate through the limiting holes to extrude the silica gel membrane; the length of the punching needle 241 is 0.2-1 mm longer than the depth of the limiting hole 231, so that the punching needle can penetrate through the limiting hole and extrude the silicon membrane in the extrusion process. Further, the longitudinal section of the membrane fastening plate 23 is T-shaped, and includes a clamping portion 232 located at the upper portion and a limiting portion 233 located at the lower portion; the size of the limiting part 233 is matched with that of the spray hole cavity 212; a clamping table 25 is formed between the spray hole cavity 212 and the spray hole base block 211, the size of the clamping table 25 is matched with the size of the area of the clamping part exceeding the limiting part, and the membrane fastening plate 23 is clamped on the clamping table 25 through the clamping part 232 of the membrane fastening plate, so that the membrane fastening plate 23 is limited in the spray hole cavity 212 in the array spray hole module.
In one embodiment, power component 3 is the electromagnetism power component, including installing electromagnet housing 31 and the electromagnet core 32 above the shower nozzle, and supporting seat 5 that is used for fixed electromagnet housing is installed at the top of upper cover plate 12, and second screw hole 6 has been seted up along the horizontal direction to the lateral wall of supporting seat 5, and electromagnet housing 31 is fixed in the inside of supporting seat 5 through the bolt of wearing to establish in second screw hole 6 to make the impact height of electromagnet convenient. When the electromagnet core 31 moves downwards, it can apply a downward acting force to the force conduction positioning column 4, and the array punching pin 24 is synchronously driven to move in the downward movement process of the force conduction positioning column 4. It should be noted that, the power assembly can be replaced by a piezoelectric column as a power source, which can generate downward impact force, so as to extrude the array nozzle assembly and complete the sample application process. Those skilled in the art can select a suitable power source as the electromagnetic power assembly in the present application according to the implementation requirement, and the electromagnetic power assembly can be applied to the present solution as long as it can generate downward impact force.
The working process of the sample application device provided by the invention is as follows:
when the electromagnet is switched on at a high level, the electromagnet core 32 impacts the conducting locating column 4 downwards, the force conducting locating column 4 impacts the silica gel diaphragm 22, the silica gel diaphragm 22 deforms downwards, the pressure of the extrusion cavity 213 increases suddenly, and a reagent (biological sample) to be spotted in the extrusion cavity is ejected out of the jet hole 214 in a liquid drop shape; when the electromagnet is switched on at a low level, the electromagnet core 32 is lifted upwards, the silicone diaphragm 22 is deformed and restored, the pressure of the extrusion cavity 213 is suddenly reduced, and a reagent to be spotted in the liquid storage cavity based on the capillary sample injection principle is filled into the extrusion cavity 213, namely a complete injection process; repeating the above steps to perform subsequent sample application.
It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Claims (10)
1. An array type biochip spotting device, which is characterized in that: the nozzle comprises a nozzle, an array spray orifice assembly arranged in the nozzle and a power assembly arranged above the nozzle and used for applying downward impact force to the array spray orifice assembly; wherein:
the sprayer comprises a lower cover plate and an upper cover plate for covering the lower cover plate, wherein a first cavity for accommodating the array spray hole assembly is arranged in the middle of the lower cover plate, and a through sprayer through hole is formed in the middle of the first cavity; the middle part of the upper cover plate is provided with a through guide hole; a force conduction positioning column is arranged in the guide hole and is positioned right below the bottom of the power assembly;
the array spray hole assembly comprises an array spray hole module, a silica gel membrane and an array punching needle; the array spray hole module comprises a spray hole base block, the middle part of the spray hole base block is inwards sunken to form a spray hole cavity, the middle part of the spray hole cavity is provided with a plurality of extrusion cavities which are arranged in an array mode, and the middle part of each extrusion cavity is a spray hole penetrating through the spray hole base block; the extrusion cavity is provided with a plurality of liquid storage cavities for storing biological samples, the number of the liquid storage cavities is the same as that of the extrusion cavities, and each liquid storage cavity is communicated with the extrusion cavity through a flow channel arranged in the orifice base block; the silica gel diaphragm is positioned right above the extrusion cavity and used for sealing the extrusion cavity; the array punching needle is located right above the silica gel membrane, the bottom of the array punching needle is provided with punching needle heads in one-to-one correspondence to the extrusion cavities, and the punching needle heads can extrude the silica gel membrane under the downward action generated by the power assembly.
2. An array biochip spotting device according to claim 1, wherein: the array spray hole assembly further comprises a membrane fastening plate used for pressing the silica gel membrane, and the membrane fastening plate is located between the array punching needle and the silica gel membrane; the middle part of the diaphragm fastening plate is provided with limiting holes which correspond to the punching heads one by one, and the punching heads are in clearance fit with the limiting holes and can penetrate through the limiting holes to extrude the silica gel diaphragm; the length of the punching needle head is 0.2-1 mm longer than the depth of the limiting hole.
3. An array biochip spotting device according to claim 2, wherein: the longitudinal section of the membrane fastening plate is T-shaped and comprises a clamping part positioned at the upper part and a limiting part positioned at the lower part; the size of the limiting part is matched with that of the spray hole cavity; a clamping table is formed between the spray hole cavity and the spray hole base block, and the size of the clamping table is matched with the size of an area of the clamping portion, which exceeds the limiting portion.
4. An array biochip spotting device according to claim 1, wherein: the middle of the bottom surface of the upper cover plate is provided with a second cavity matched with the array punching needle in shape, and the guide hole is located in the middle of the upper portion of the second cavity and communicated with the second cavity.
5. An array biochip spotting apparatus according to claim 4, wherein: the middle of the top surface of the array punching needle is provided with a positioning groove, and the shape of the positioning groove is matched with the shape of the bottom of the force transmission positioning column.
6. An array biochip spotting device according to claim 1, wherein: the number of the extrusion cavities in the array spray hole module is 1-48, and the distance between two adjacent spray holes is 0.1-5 mm.
7. An array biochip spotting device according to claim 1, wherein: the side of week of apron and upper cover plate down corresponds and has seted up the first screw hole of a plurality of, apron and upper cover plate are through wearing to establish the bolt fixed connection in the first screw hole down.
8. An array biochip spotting apparatus according to any one of claims 1 to 7, wherein: the flow channel is provided with a flow restraining structure at one end close to the extrusion cavity, the flow restraining structure is in a boss shape, the flow restraining structure enables the liquid flux at the position, close to the liquid storage cavity, of the flow channel to be small, and liquid in the extrusion cavity is reduced to flow back to the liquid storage cavity along the flow channel in the process that the needle punching head extrudes the silica gel membrane.
9. An array biochip spotting device according to claim 8, wherein: the power assembly is an electromagnetic power assembly and comprises an electromagnet shell and an electromagnet core, wherein the electromagnet shell and the electromagnet core are installed above the spray head, downward acting force can be applied to the force conduction positioning column when the electromagnet core moves downwards, and the force conduction positioning column synchronously drives the array punching needle to move in the downward movement process.
10. An array biochip spotting device according to claim 9, wherein: the supporting seat that is used for fixed electromagnet shell is installed at the top of upper cover plate, the second screw hole has been seted up along the horizontal direction to the lateral wall of supporting seat, electromagnet shell is through wearing to establish the inside at the supporting seat of the bolt fastening in second screw hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211066755.2A CN115452507B (en) | 2022-09-01 | Array biochip sample application device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211066755.2A CN115452507B (en) | 2022-09-01 | Array biochip sample application device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115452507A true CN115452507A (en) | 2022-12-09 |
| CN115452507B CN115452507B (en) | 2024-05-14 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102649931A (en) * | 2012-05-28 | 2012-08-29 | 上海理工大学 | Preparation method for microarray biochip |
| CN202705345U (en) * | 2012-05-28 | 2013-01-30 | 上海理工大学 | Microarray biochip preparation device |
| KR20180038743A (en) * | 2016-10-07 | 2018-04-17 | 주식회사 제타 | Bio-Chip Arrayer |
| KR102078567B1 (en) * | 2018-10-01 | 2020-02-19 | 주식회사 이바이오젠 | Non-contact microarrayer using image recognition technology |
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102649931A (en) * | 2012-05-28 | 2012-08-29 | 上海理工大学 | Preparation method for microarray biochip |
| CN202705345U (en) * | 2012-05-28 | 2013-01-30 | 上海理工大学 | Microarray biochip preparation device |
| KR20180038743A (en) * | 2016-10-07 | 2018-04-17 | 주식회사 제타 | Bio-Chip Arrayer |
| KR102078567B1 (en) * | 2018-10-01 | 2020-02-19 | 주식회사 이바이오젠 | Non-contact microarrayer using image recognition technology |
Non-Patent Citations (2)
| Title |
|---|
| 赵启焱;尤晖;郑敏捷;黄哲;: "新型非接触式微液滴点样喷头的研制", 仪表技术, no. 09, 15 September 2017 (2017-09-15) * |
| 郑敏捷;谢洋;赵启焱;尤晖;: "微阵列芯片点样微喷系统研制", 机械研究与应用, no. 03, 28 June 2018 (2018-06-28) * |
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