CN112063494A

CN112063494A - Sealed high-flux efficient full-automatic biosynthesis device

Info

Publication number: CN112063494A
Application number: CN202011107557.7A
Authority: CN
Inventors: 周郭丹玉; 吕永维; T·德米特; 田敬东
Original assignee: Jiangsu Dongxuan Gene Technology Co ltd
Current assignee: Jiangsu Dongxuan Gene Technology Co ltd
Priority date: 2020-10-16
Filing date: 2020-10-16
Publication date: 2020-12-11

Abstract

The invention discloses a sealed high-flux high-efficiency full-automatic biosynthesis device which comprises a moving plate, a plurality of synthesis cavity units, a sealing plate assembly, a sample adding unit and a plurality of pressure sealing units, wherein the moving plate performs horizontal linear motion, the synthesis cavity units are fixed below the moving plate and form an upper opening on the moving plate, the sealing plate assembly is positioned above the moving plate and forms a sealed space with the moving plate through up-and-down motion, the sample adding unit is fixed on the sealing plate assembly, the pressure sealing units are fixed on the sealing plate assembly and seal the upper opening, and the moving plate can move between the position of the sample adding unit and the position of the pressure sealing units under the drive of a driving piece. The invention realizes the automatic addition of sample amount and the automatic transferring of primers into a sealed reaction cabin in the process of large-batch ultramicro biological sample synthesis reaction, and performs automatic pressurization and pressure maintaining, precisely controls the pressure and synthesis speed required by synthesis, and greatly improves the biosynthesis efficiency.

Description

Sealed high-flux efficient full-automatic biosynthesis device

[ technical field ] A method for producing a semiconductor device

The invention belongs to the technical field of biosynthesis devices, and particularly relates to a sealed high-flux high-efficiency full-automatic biosynthesis device.

[ background of the invention ]

At present, a DNA synthesis column usually adopts a single-column tube or a 96-hole filter plate, the existing single-column tube has multiple specifications and large volume, is not beneficial to batch, large-scale and miniaturized operation, and is difficult to realize for trace, ultra-small and large-batch DNA synthesis; the existing 96-hole filter plate mainly has two specifications of 1.5 and 2ml, can finish the synthesis work of 96 DNA samples, is quicker and more than a single tube for the synthesis of mass DNA, and still cannot meet the mass and scale synthesis of trace and ultra-trace DNA in terms of the sample amount to be processed. Therefore, 384-well plates are developed in the market, and the synthesis of 384 DNA samples can be completed simultaneously. However, the sample adding of 384-well plates is a very huge work, and in the prior art, no sample adding device exists, which can be matched with the high-density sample adding of 384-well plates, so that the sample adding efficiency is low, and the time required for synthesis is long. In the synthesis process, the synthesis plate loaded reagent needs to be in a sealed environment according to the requirements of synthesis conditions, and needs to be filled with inert gas for protection, but in the prior art, the sealing performance of a synthesized sealed chamber is poor, and when a sample and the reagent are added, all manual operation is adopted, so that the workload is very large, the efficiency is extremely low, the sample amount control precision is poor, the synthesis conditions are unstable, and the synthesis efficiency is poor.

Therefore, it is necessary to provide a new sealed type high-throughput high-efficiency fully automatic biosynthesis apparatus to solve the above problems.

[ summary of the invention ]

The invention mainly aims to provide a sealed high-flux high-efficiency full-automatic biosynthesis device, which realizes automatic addition of sample amount and automatic transfer of primers into a sealed reaction cabin in the process of large-batch ultramicro biological sample synthesis reaction, performs automatic pressurization and pressure maintaining, precisely controls the pressure and synthesis speed required by synthesis and greatly improves the biosynthesis efficiency.

The invention realizes the purpose through the following technical scheme: a sealed high-flux high-efficiency full-automatic biosynthesis device comprises a moving plate, a plurality of synthesis cavity units, a sealing plate assembly, a sample adding unit and a plurality of pressure sealing units, wherein the moving plate moves horizontally and linearly, the synthesis cavity units are fixed below the moving plate and form an upper opening on the moving plate, the sealing plate assembly is positioned above the moving plate and forms a sealed space with the moving plate through up-and-down movement, the sample adding unit is fixed on the sealing plate assembly, the pressure sealing units are fixed on the sealing plate assembly and seal the upper opening, and the moving plate is driven by a driving piece to move between the position of the sample adding unit and the position of the pressure sealing units.

Furthermore, the synthetic cavity unit comprises a liquid drainage collecting box and a synthetic plate which is positioned at the top of the liquid drainage collecting box and is communicated with the inner cavity of the liquid drainage collecting box;

the liquid discharge collecting box is positioned below the upper opening, and the top of the surrounding baffle is hermetically attached to the lower surface of the moving plate, so that a sealed space is formed below the synthetic plate.

Furthermore, a plurality of synthetic holes are arranged on the synthetic plate, the upper parts of the synthetic holes are square openings, the synthetic holes are sequentially divided into a fourth hole section and a fifth hole section from top to bottom, the fourth hole section is an inverted cone-shaped hole or a square hole, and the fifth hole section is a cylindrical hole or an inverted cone-shaped hole.

Further, the sealing plate assembly comprises a sealing support plate covering the upper portion of the moving plate, a first sealing ring fixed on the lower surface of the sealing support plate, and a driving cylinder driving the sealing support plate to move up and down.

Furthermore, the application of sample unit includes the backup pad, arranges and sets up a plurality of distributing pipe mount pads in the backup pad, arrange and set up a plurality of distributing pipes on the distributing pipe mount pad, the other end and the automatic priming device intercommunication of distributing pipe.

Furthermore, be provided with a plurality of first mounting holes that run through upper and lower surface on the distributing pipe mount pad, first mounting hole top-down divide into first hole section, second hole section and third hole section in proper order, the second hole section is the screw hole, the distributing pipe be close to the lower part region be provided with one section with screw hole complex external screw thread structure.

Furthermore, an insertion pipe is inserted into the lower end of the distribution pipe, the third hole section is a tapered hole with a large upper part and a small lower part, and the third hole section extrudes the lower section area of the distribution pipe to seal the distribution pipe and the insertion pipe by utilizing the tapered hole structure and tightly holds the insertion pipe to realize the fixed sealing connection of the distribution pipe and the insertion pipe.

Furthermore, the pressurizing and sealing unit comprises a frame plate support fixed on the sealing plate assembly, driving cylinders fixed on four corner regions of the frame plate support, a sealing and pressurizing module driven by the driving cylinders to move up and down, and a second sealing ring for sealing a contact region between the lower surface of the sealing and pressurizing module and the peripheral surface of the opening at the upper part of the synthesis cavity unit.

Furthermore, sealed pressurization module is including the upper padding plate and the lower bolster that are the rectangle frame structure, set up the upper padding plate with observation luffer boards between the lower bolster, right the upper padding plate with observe luffer boards contact site and carry out the third sealing washer sealed, right the lower bolster with observe luffer boards contact site and carry out the fourth sealing washer sealed.

Furthermore, the lower backing plate and the observation window plate enclose and block together to form a pressurizing chamber with a downward opening, the upper backing plate and the lower backing plate are internally provided with a pressurizing air passage communicated with the pressurizing chamber, and the pressurizing air passage is communicated with a pressurizing device pipeline.

Compared with the prior art, the sealed high-flux high-efficiency full-automatic biosynthesis device has the beneficial effects that: the automatic liquid adding and automatic pressurizing sealing synthesis of large-batch and ultra-micro nucleic acid synthesis are realized, the synthesis efficiency and the synthesis quality are greatly improved, the pressure of a reaction sealed cabin can be flexibly controlled according to the requirements of reaction conditions, and the time of liquid flowing through a synthesis carrier is controlled; the sample adding station and the synthesis reaction station are independently arranged, so that the problem of liquid dripping at the bottom of a sample adding nozzle is effectively solved, the sealing performance of a biosynthesis reaction environment is greatly improved through the arrangement of the sealing plate assembly and the pressurizing sealing unit, and the effective operation of the synthesis reaction is guaranteed; the distribution pipe is provided with the distribution pipe external thread and the distribution pipe mounting seat structure, so that the distribution pipe arrangement density is greatly improved; the problem that the distribution space of the original pipe fitting pipeline shape is large is solved; the general pipe fitting cannot realize 4.5-4.5 mm matrix distribution; and the manufacturing cost is expensive; the structural parts of the scheme improve the manufacturability of the system, and the distributed pressure layout solves the pressure load at the position of a distributed pipeline, so that a simple mechanism realizes sealing; by arranging the high-density distribution pipes, the synthesis efficiency is greatly improved, and the density of the distribution pipes is high; the displacement distance of the moving plate can be effectively reduced, the single operation time is reduced, and the synthesis efficiency is improved; meanwhile, the number of distribution paths is effectively increased, more reagent types can be provided at the same time on the same distance, and large-flux synthesis is realized through algorithm optimization; the equipment size is greatly reduced by arranging the high-density distribution pipes; the sizes of the pipe fitting parts are controlled within the range of the universal processing capacity; the processing cost is greatly reduced; meanwhile, effective space control can provide sufficient design possibility for other components; finally, a more stable structure is optimized; the synthesis in the positive pressure range is realized by setting the pressure distribution of different areas; the pressure of public works is greatly reduced; no negative pressure and large amount of waste gas and waste liquid treatment equipment are needed.

[ description of the drawings ]

FIG. 1 is a schematic structural diagram of an embodiment of the present invention;

FIG. 2 is a schematic structural view of a stent according to an embodiment of the present invention;

FIG. 3 is a schematic structural diagram of a moving plate and a synthesis cavity unit according to an embodiment of the present invention;

FIG. 4 is a schematic structural diagram of a synthesis chamber unit according to an embodiment of the present invention;

FIG. 5 is a schematic view of a composite sheet according to an embodiment of the present invention in a partial cross-sectional configuration;

FIG. 6 is a schematic diagram of an assembly structure of a sealing plate assembly, a sample adding unit and a pressure sealing unit according to an embodiment of the present invention;

FIG. 7 is an exploded view of the sealing plate assembly, the sample loading unit and the pressure sealing unit according to the embodiment of the present invention;

FIG. 8 is a schematic structural diagram of a sample adding unit in an embodiment of the present invention;

FIG. 9 is a schematic view of a portion of a sample loading assembly according to an embodiment of the present disclosure;

FIG. 10 is an enlarged partial view of a dispensing tube mount according to an embodiment of the present invention;

FIG. 11 is a schematic view, partially in section, of an embodiment of the invention after a dispensing tube and a cannula have been assembled to a dispensing tube mount;

FIG. 12 is a schematic structural view of a pressurized sealing unit according to an embodiment of the present invention;

FIG. 13 is a schematic cross-sectional view of a pressurized sealing unit according to an embodiment of the present invention;

the figures in the drawings represent:

100 sealing type high-flux high-efficiency full-automatic biosynthesis device;

1, moving a plate, 11 is opened at the upper part; 2 synthetic cavity unit, 21 liquid discharge collecting box, 22 synthetic plate, 221 synthetic hole, 222 fourth hole section, 223 fifth hole section, 224 synthetic carrier, 23 supporting frame plate, 24 door baffle, 25 observation window, 26 waste liquid collecting box;

3, a sealing plate assembly, 31 a sealing support plate, 32 a first sealing ring and 33 a driving cylinder;

4, a sample adding unit, a 41 support plate, 411 strip-shaped holes, 42 distribution pipe installation seats, 421 first installation holes, 4211 first hole sections, 4212 second hole sections, 4213 third hole sections, 422 strip-shaped support parts, 423 limiting guide parts, 424 second installation holes, 43 distribution pipes, 431 external thread structures and 44 insertion pipes;

5, a pressure sealing unit, 51, a frame plate bracket, 52, a driving cylinder, 53, a pressure sealing module, 531, a lower cushion plate, 532, an observation window plate, 534, a third sealing ring, 535, a fourth sealing ring, 536, a bolt group, 537, a pressure air channel, 538, 54, a second sealing ring; 6 driving part; 7, a support seat, 71 supporting the roller group and 72 limiting the stop ring.

[ detailed description ] embodiments

Example (b):

referring to fig. 1 to 13, the present embodiment is a sealed high-throughput high-efficiency fully-automatic biosynthesis apparatus 100, which includes a moving plate 1 performing a horizontal linear motion, a plurality of synthesis chamber units 2 fixed below the moving plate 1 and forming an upper opening 11 on the moving plate 1, a sealing plate assembly 3 located above the moving plate 1 and forming a sealed space with the moving plate 1 by moving up and down, a sample adding unit 4 fixed on the sealing plate assembly 3, and a plurality of pressure sealing units 5 fixed on the sealing plate assembly 3 and sealing the upper opening 11, wherein the moving plate 1 is driven by a driving member 6 to move between a position of the sample adding unit 4 and a position of the independent pressure sealing unit 5.

The embodiment further comprises a support 7, the support 7 is provided with a supporting roller set 71, and the moving plate 1 is erected on the supporting roller set 71 to move horizontally. Two ends of the support 7 are also provided with limit stop rings 72, so that no obstacle exists in the moving range of the moving plate 1, and the safety is improved.

The synthetic cavity unit 2 comprises a drainage collecting box 21 and a synthetic plate 22 which is positioned at the top of the drainage collecting box 21 and is communicated with the inner cavity of the drainage collecting box 21. The composite plate 22 is fixed at the upper opening 11 by a support frame plate 23. The liquid discharge collection box 21 is positioned below the upper opening 11, and the top of the periphery enclosure is arranged in sealing contact with the lower surface of the moving plate 1, so that a sealed space is formed below the synthetic plate 22. An openable door baffle 24 is arranged on one side of the liquid drainage collection box 21, and an observation window 25 is arranged on the door baffle 24, so that the condition in the liquid drainage collection box 21 can be observed conveniently.

In this embodiment, synthesis chamber unit 2 is provided with two sets ofly, and still is provided with a waste liquid between two adjacent synthesis chamber units 2 and collects box 26, and the effect that box 26 was collected to the waste liquid has:

1) providing a blank group setting space in the debugging process; no personnel is required to be arranged to independently take liquid;

2) the synthesis process provides a liquid discharge space before cleaning and primary liquid discharge; pre-draining liquid in the dead zone; preventing the occurrence of bubbles and conditions which are not in the control range;

3) the liquid outlet condition can be conveniently observed in the synthesis process;

4) optimizing the process and the program structure and providing an air defense position for the process.

The composite plate 22 is a 384-well plate or a 96-well plate or other number of well plates. The synthetic plate 22 is provided with a plurality of synthetic holes 221 in an array, the upper portions of the synthetic holes 221 are square openings, the synthetic holes 221 are sequentially divided into a fourth hole section 222 and a fifth hole section 223 from top to bottom, the fourth hole section 222 is an inverted cone-shaped hole or a square hole, and the fifth hole section 223 is a cylindrical hole or an inverted cone-shaped hole. In the case of DNA nucleic acid synthesis, the synthesis vector 224 is placed in the synthesis well 221. The synthesis carrier 224 is a cylindrical solid phase synthesis carrier, and is pushed into the tip end downwards along the square opening, and the bottom end of the synthesis carrier 224 is fixed in the fifth hole section 223 and the top end extends into the fourth hole section 222.

The sealing plate assembly 3 includes a sealing support plate 31 covering the upper side of the moving plate 1, a first sealing ring 32 fixed to the lower surface of the sealing support plate 31, and a driving cylinder 33 driving the sealing support plate 31 to move up and down. The first sealing ring 32 is abutted with the surface of the moving plate 1 along with the descending of the sealing support plate 31 to form a sealing space, so that the upper spaces of sections of the sample adding unit 4 and the pressurizing sealing unit 5 covered by the moving plate 1 are sealed, and the synthetic plate 22 is positioned in a completely sealed environment by matching with the liquid drainage collection box 21 below the moving plate 1, so that the biosynthesis environment is improved, and the time of exposing a reagent and a sample in the air is greatly shortened.

The sample adding unit 4 comprises a supporting plate 41, a plurality of distributing pipe mounting seats 42 arranged on the supporting plate 41, and a plurality of distributing pipes 43 arranged on the distributing pipe mounting seats 42, wherein the other ends of the distributing pipes 43 are communicated with an automatic liquid injection device (not shown in the figure). The distribution pipes 43 are provided in one-to-one correspondence with the combining holes 221 in the combining plate 22. The distribution pipe mounting seat 42 is provided with a plurality of first mounting holes 421 penetrating through the upper and lower surfaces, the first mounting holes 421 are sequentially divided into a first hole section 4211, a second hole section 4212 and a third hole section 4213 from top to bottom, the second hole section 4212 is a threaded hole, the third hole section 4213 is a tapered hole with a large top and a small bottom, and the distribution pipe 43 is provided with a section of external thread structure 431 matched with the threaded hole near the lower region. The first bore section 4211 has a larger inner diameter than the second bore section 4212. The automatic liquid injection device can be an existing structure on the market.

The dispensing tube mounting seats 42 are strip-shaped structures and are arranged one by one or at intervals on the support plate 41. The dispensing tube mounting seat 42 includes a bar-shaped supporting portion 422, and a position-limiting guide portion 423 located on a lower surface of the bar-shaped supporting portion 422. The area enclosed by the outer contour of the position-limiting guide part 423 is located within the area enclosed by the outer contour of the strip-shaped support part 422. The supporting plate 41 is provided with a plurality of strip holes 411 for the limiting guide parts 423 to pass through.

The lower end of the dispensing tube 43 is inserted with a insertion tube 44, the upper end of the insertion tube 44 is inserted in the inner hole of the dispensing tube 43 and the bottom end extends to protrude out of the lower surface of the mounting seat 42 of the dispensing tube. The third hole segment 4213 extrudes the lower section area of the distribution pipe 43 by utilizing the conical hole structure thereof to seal the distribution pipe with the insertion pipe 44, and tightly holds the insertion pipe 44 to realize the fixed sealing connection of the insertion pipe 44 and the insertion pipe 44, so that the insertion pipe 44 is prevented from slipping and moving downwards, and the extending length of the insertion pipe 44 is ensured to be kept unchanged. When the inner diameter of the distribution pipe 43 is small, the sample addition operation may be performed without inserting the cannula 44.

The pressure sealing unit 5 includes a frame plate support 51 fixed to the seal support plate 31, a driving cylinder 52 fixed to four corner regions of the frame plate support 51, a sealing pressure module 53 driven by the driving cylinder 52 to move up and down, and a second sealing ring 54 sealing a contact region of a lower surface of the sealing pressure module 53 and a peripheral surface of an upper opening of the synthesis chamber unit 2. The sealing and pressurizing module 53 includes an upper pad 531 and a lower pad 532 having a rectangular frame structure, a viewing window plate 533 provided between the upper pad 531 and the lower pad 532, a third seal ring 534 sealing a contact portion of the upper pad 531 and the viewing window plate 533, a fourth seal ring 535 sealing a contact portion of the lower pad 532 and the viewing window plate 533, and a plurality of bolt groups 536 locking the upper pad 531 and the lower pad 532. The lower base plate 532 and the observation window plate 533 enclose to form a pressure chamber 538 with a downward opening, the upper base plate 531 and the lower base plate 532 are internally provided with a pressure air channel 537 which is communicated with the pressure chamber 538, and the pressure air channel 537 is communicated with a pressure device (not shown in the figure) through a pipeline. The pressurizing device can be of the structure existing on the market.

The upper surface of the sealing and pressurizing module 53 is closely attached to the inner wall surface of the panel holder 51 at the upper limit height position. In other embodiments, a fifth gasket (not shown) may be provided to seal the contact area of the compression module 53 against the frame plate support 51.

The pressurizing and sealing units 5 in this embodiment are provided in two sets, one-to-one corresponding to the two sets of synthesis chamber units 2.

The working principle of the sealed high-flux high-efficiency full-automatic biosynthesis device 100 of the embodiment is as follows: in the initial state, the seal support plate 31 is located at the upper limit height position; the synthesis cavity unit 2 moves to the lower part of the sample adding unit 4 under the driving action of the driving piece 6; the sealing support plate 31 descends to press the surface of the moving plate 1, and the upper space of the moving plate 1 is sealed by the first sealing ring 32; the distribution pipe 43 in the sample adding unit 4 is communicated through an external automatic liquid injection device, a reagent or a sample required by the reaction is added, the reagent or the sample is dripped into the synthetic plate 22 through the tail end of the distribution pipe 43, and one group or two groups of 384-part samples can be synchronously added at one time through the sample adding unit 4; after the sample is added, the sealing support plate 31 is lifted, the driving member 6 drives the synthesis cavity unit 2 to move to the lower part of the pressurizing and sealing unit 5, the sealing support plate 31 is lowered, meanwhile, the sealing and pressurizing module 53 is lowered to cover the upper opening of the synthesis plate 22, inert gas or other protective gas is blown in through the pressurizing device and enters the pressurizing chamber 538 through the pressurizing air channel 537, so that the synthesis plate 22 is placed in the sealed space with inert gas protection and pressurized environment, the synthesis condition can be observed through the observation window 533, the synthesized reagent is discharged from the end of the bottom synthesis carrier 224 of the synthesis plate 22 into the liquid discharge and collection box 21, and the synthesized reagent can be taken out by opening the door baffle 24.

The sealed high-flux high-efficiency full-automatic biosynthesis device 100 has the advantages that: the automatic liquid adding and automatic pressurizing sealing synthesis of large-batch and ultra-micro nucleic acid synthesis are realized, the synthesis efficiency and the synthesis quality are greatly improved, the pressure of a reaction sealed cabin can be flexibly controlled according to the requirements of reaction conditions, and the time of liquid flowing through a synthesis carrier is controlled; the sample adding station and the synthesis reaction station are independently arranged, so that the problem of liquid dripping at the bottom of a sample adding nozzle is effectively solved, the sealing performance of a biosynthesis reaction environment is greatly improved through the arrangement of the sealing plate assembly and the pressurizing sealing unit, and the effective operation of the synthesis reaction is guaranteed; the distribution pipe is provided with the external threads and the distribution plate structure, so that the distribution pipe arrangement density is greatly improved; the problem that the distribution space of the original pipe fitting pipeline shape is large is solved; by the arrangement of the double cavities, various modes can be switched; the synthesis amount or synthesis type is greatly improved; two cavities are synchronously synthesized; or synthesized separately; the synthesis efficiency is improved; the sealing device between the moving plate and the distribution plate is arranged; the sealing charge degree is greatly reduced, and effective sealing in a moving state is realized; the cam follower greatly improves the manufacturability of the equipment and provides a flexible moving space for a long-stroke moving plate; meanwhile, the cost is reduced; the cavity window is arranged, so that the synthesis controllability is greatly improved; the synthesis process can be monitored in time through four windows; the synthesis quality is ensured.

What has been described above are merely some embodiments of the present invention. It will be apparent to those skilled in the art that various changes and modifications can be made without departing from the inventive concept thereof, and these changes and modifications can be made without departing from the spirit and scope of the invention.

Claims

1. The utility model provides a full-automatic biosynthetic apparatus of canned type high flux high efficiency which characterized in that: the device comprises a moving plate, a plurality of synthesis cavity units, a sealing plate assembly, a sample adding unit and a plurality of pressure sealing units, wherein the moving plate performs horizontal linear motion, the synthesis cavity units are fixed below the moving plate and form an upper opening on the moving plate, the sealing plate assembly is positioned above the moving plate and forms a sealing space with the moving plate through up-and-down motion, the sample adding unit is fixed on the sealing plate assembly, the pressure sealing units are fixed on the sealing plate assembly and seal the upper opening, and the moving plate can move between the position of the sample adding unit and the position of the pressure sealing units under the drive of a driving.

2. The sealed high-throughput highly-efficient fully-automated biosynthetic apparatus of claim 1, wherein: the synthetic cavity unit comprises a liquid discharge collecting box and a synthetic plate which is positioned at the top of the liquid discharge collecting box and is communicated with the inner cavity of the liquid discharge collecting box;

3. The sealed high-throughput highly-efficient fully-automated biosynthetic apparatus of claim 2, wherein: the composite plate is provided with a plurality of composite holes in an arrayed mode, the upper portions of the composite holes are square openings, the composite holes are sequentially divided into a fourth hole section and a fifth hole section from top to bottom, the fourth hole section is an inverted cone-shaped hole or a square hole, and the fifth hole section is a cylindrical hole or an inverted cone-shaped hole.

4. The sealed high-throughput highly-efficient fully-automated biosynthetic apparatus of claim 1, wherein: the sealing plate assembly comprises a sealing support plate covering the upper part of the moving plate, a first sealing ring fixed on the lower surface of the sealing support plate, and a driving cylinder driving the sealing support plate to move up and down.

5. The sealed high-throughput highly-efficient fully-automated biosynthetic apparatus of claim 1, wherein: the sample adding unit comprises a supporting plate, a plurality of distributing pipe mounting seats arranged on the supporting plate, and a plurality of distributing pipes arranged on the distributing pipe mounting seats, wherein the other ends of the distributing pipes are communicated with an automatic liquid injection device.

6. The sealed high-throughput highly-efficient fully-automated biosynthetic apparatus of claim 5, wherein: the distributing pipe mounting seat is provided with a plurality of first mounting holes penetrating through the upper surface and the lower surface, the first mounting holes are sequentially divided into a first hole section, a second hole section and a third hole section from top to bottom, the second hole section is a threaded hole, and the distributing pipe is provided with a section of external thread structure matched with the threaded hole near the lower part area.

7. The sealed high-throughput highly-efficient fully-automated biosynthetic apparatus of claim 6, wherein: the lower end of the distribution pipe is inserted with an insertion pipe, the third hole section is a tapered hole with a large upper part and a small lower part, and the third hole section extrudes the lower section area of the distribution pipe by utilizing the tapered hole structure to seal the distribution pipe and the insertion pipe and tightly holds the insertion pipe to realize the fixed sealing connection of the distribution pipe and the insertion pipe.

8. The sealed high-throughput highly-efficient fully-automated biosynthetic apparatus of claim 1, wherein: the pressurizing and sealing unit comprises a frame plate support fixed on the sealing plate assembly, driving cylinders fixed on four corner regions of the frame plate support, a sealing and pressurizing module driven by the driving cylinders to move up and down, and a second sealing ring for sealing a contact region between the lower surface of the sealing and pressurizing module and the peripheral surface of an opening at the upper part of the synthesis cavity unit.

9. The sealed high-throughput highly-efficient fully-automated biosynthetic apparatus of claim 8, wherein: the sealing and pressurizing module comprises an upper base plate and a lower base plate which are of a rectangular frame structure, an observation window plate arranged between the upper base plate and the lower base plate, a third sealing ring for sealing the upper base plate and the observation window plate contact part, and a fourth sealing ring for sealing the lower base plate and the observation window plate contact part.

10. The sealed high-throughput highly-efficient fully-automated biosynthetic apparatus of claim 9, wherein: the lower base plate and the observation window plate are jointly enclosed to form a pressurizing chamber with a downward opening, the upper base plate and the lower base plate are internally provided with pressurizing air passages communicated with the pressurizing chamber, and the pressurizing air passages are communicated with a pressurizing device pipeline.