CN110408518B - A compress tightly sealed flowing back device that blows for on DNA synthesizer - Google Patents
A compress tightly sealed flowing back device that blows for on DNA synthesizer Download PDFInfo
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- CN110408518B CN110408518B CN201910776414.6A CN201910776414A CN110408518B CN 110408518 B CN110408518 B CN 110408518B CN 201910776414 A CN201910776414 A CN 201910776414A CN 110408518 B CN110408518 B CN 110408518B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Abstract
The invention relates to a compacting, sealing and blowing liquid discharging device for a DNA synthesizer, which comprises a cylinder body and a piston assembly, wherein the piston assembly is arranged in the cylinder body in a sliding manner, a first cavity is formed between the upper part of the piston assembly and the interior of the cylinder body, the outer side surface of the piston assembly is in sealing connection with the inner surface of the cylinder body, a first through hole and a second through hole are formed in the cylinder body, the first through hole is communicated with the first cavity, a third through hole is formed in the inner side of the piston assembly, and the second through hole is communicated with the third through hole. The device solves the problems that the structure of the compaction sealing blowing liquid discharging device is complex, the volume is overlarge, the restriction on the structure of an instrument is high, and the compaction and blowing can not accurately control the reaction and liquid discharging of reagents in a synthetic column.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a compacting, sealing, blowing and draining device for a DNA synthesizer.
Background
At present, the artificially synthesized DNA is the only way for the known directional modification of gene sequences, and is widely applied to a plurality of fields such as protein modification, life science and the like, such as nucleic acid medicine, enzyme engineering, gene detection, gene therapy and the like. The DNA synthesizer requires deprotection, activation linking, capping, oxidation, and the like steps during the synthesis process. In the synthesis process, different reagents are injected into the synthesis column under the protection of inert gas environment, so that the waste liquid discharge time is accelerated and the waste liquid is drained in order to make the reagents fully perform chemical reaction, and the vacuum suction or the inert gas filling pressure is required to control the permeation time of the reagents in the synthesis column, so that the waste liquid draining is accelerated. For the DNA synthesizer of 48 channels, 192 channels, 384 channels and 768 channels and even higher channels, the method needs to control the reagent permeation time in a plurality of synthesis columns to be uniform and even, compared with a vacuumizing mode, the method of filling inert gas to generate pressure generates larger and controllable pressure, can ensure the reagent permeation time in a plurality of synthesis columns to be uniform and even, can drain waste liquid in a shorter time, and greatly shortens the synthesis time of the whole sequence.
Currently, there are two main disadvantages of DNA synthesizers that use inert gas filling to generate pressure in the domestic market: 1. the sealing, compacting, blowing and draining device has a complex structure and an overlarge volume; 2. the whole device has high restriction on the structure of the instrument, and can only be used on a DNA synthesizer with a specific structure; 3. the compaction and blowing do not allow precise control of reagent reaction and drainage in the synthesis column. Therefore, a compact blowing liquid draining device with small volume, high applicability and good sealing performance is required to control the reagent permeation time and the drained waste liquid.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a compacting and sealing blowing liquid discharging device for a DNA synthesizer, which solves the problems that the compacting and sealing blowing liquid discharging device has complex structure and overlarge volume, has high restriction on the structure of the instrument and can not accurately control the reaction and liquid discharging of reagents in a synthetic column.
The technical scheme for solving the technical problems is as follows: the utility model provides a compress tightly sealed flowing back device that blows for on DNA synthesizer, includes cylinder body 1 and piston assembly 2, piston assembly 2 slides and sets up inside the cylinder body 1, piston assembly 2 upper portion with the inside first cavity that forms of cylinder body 1, piston assembly 2 the lateral surface with the internal surface sealing connection of cylinder body 1, first through-hole 3 and second through-hole 4 have been seted up on the cylinder body 1, first through-hole 3 with first cavity is linked together, the third through-hole has been seted up to piston assembly 2 inboard, second through-hole 4 with the third through-hole is linked together.
Further, the device also comprises an elastic fixing component 5, wherein the edge of the elastic fixing component 5 is fixedly connected with the lower part of the cylinder body 1, and the elastic fixing component 5 is elastically connected with the lower surface of the piston component 2.
Further, the elastic fixing assembly 5 comprises a spring 5.3 fixing support 5.1 and a plurality of springs 5.3, the spring 5.3 fixing support 5.1 is provided with an L-shaped groove 5.2, the springs 5.3 are uniformly distributed inside the L-shaped groove 5.2, the springs 5.3 are fixedly connected with the spring 5.3 fixing support 5.1, the upper ends of the springs 5.3 are propped against the lower surface of the piston assembly 2, and the springs 5.3 fixing support 5.1 are fixedly connected with the lower portion of the cylinder body 1.
Further, a through hole is formed in the upper portion of the cylinder body 1, and an upper perspective plate 6 is arranged at the position of the through hole in a sealing manner.
Further, the piston assembly 2 comprises a middle perspective plate 2.1, a rubber sealing ring 2.2, a silica gel fixing plate 2.5 and a silica gel pad 2.6, wherein an annular groove 2.4 is formed in the outer side of the silica gel fixing plate 2.5, the rubber sealing ring 2.2 is sleeved in the annular groove 2.4, a hollow groove 2.3 is formed in the middle of the silica gel fixing plate 2.5, the middle perspective plate (2.1) is arranged at the position of the hollow groove (2.3) in a sealing mode, and the silica gel pad 2.6 is fixed on the lower surface of the silica gel fixing plate 2.5.
Further, the second through hole 4 is communicated with the third through hole through a connecting component 7, a semi-annular groove body 8 is formed in the bottom of the cylinder body 1, the connecting component 7 is arranged in the semi-annular groove body 8, one end of the connecting component 7 is in sealing connection with the second through hole 4, and the other end of the connecting component 7 is in sealing connection with the third through hole.
Further, the connecting component 7 comprises a hose 7.1, a threaded connector 7.2 and a threaded elbow 7.3, the threaded connector 7.2 and the threaded elbow 7.3 are of hollow structures, one end of the hose 7.1 is in sealing connection with the second through hole 4, the other end of the hose 7.1 is inserted into the threaded connector 7.2 in an interference manner and is in sealing connection with the threaded connector 7.2, the threaded connector 7.2 is in threaded connection with the threaded elbow 7.3, and the threaded elbow 7.3 is in sealing connection with the third through hole.
Further, the quick connector comprises a quick through threaded connector 9 and a quick reducing connector 10, the quick through threaded connector 9 is arranged on the first through hole 3 and is in threaded connection with the first through hole 3, and the quick reducing connector 10 is arranged on the second through hole 4 and is in threaded connection with the second through hole 4.
Further, the third through hole is composed of an air outlet 11 and a threaded blind hole 12, the air outlet 11 is formed in the inner side face of the silica gel fixing plate 2.5, the threaded blind hole 12 is formed in the top face of the silica gel fixing plate 2.5, and the air outlet 11 is communicated with the threaded blind hole 12.
The invention provides a compacting, sealing and blowing liquid discharging device for a DNA synthesizer, which comprises a cylinder body 1 and a piston assembly 2, wherein the piston assembly 2 is arranged in the cylinder body 1 in a sliding way, a first cavity is formed between the upper part of the piston assembly 2 and the interior of the cylinder body 1, the outer side surface of the piston assembly 2 is in sealing connection with the inner surface of the cylinder body 1, a first through hole 3 and a second through hole 4 are formed in the cylinder body 1, the first through hole 3 is communicated with the first cavity, a third through hole is formed in the inner side of the piston assembly 2, and the second through hole 4 is communicated with the third through hole. Thus, the piston assembly 2 is slidably arranged inside the cylinder 1, a first cavity is formed between the upper part of the piston assembly 2 and the inside of the cylinder 1, when the first cavity is inflated through the first through hole 3 formed in the cylinder 1, the outer side surface of the piston assembly 2 is in sealing connection with the inner surface of the cylinder 1, so that the piston assembly 2 is forced to move downwards due to the fact that the second cavity can generate great gas pressure, the synthetic plate 13 on the DNA synthesizer is further compressed, a second cavity is formed between the piston assembly 2 and the synthetic plate 13 on the DNA synthesizer, at the moment, the second cavity is inflated through the second through hole 4 formed in the cylinder 1, the communication between the second through hole 4 and the second cavity is realized through the third through hole, the third through hole is formed on the inner side surface of the piston assembly 2, and the third through hole is communicated with the second through hole 4. Therefore, the device has compact structure and small size, can generate great pressure under very small pressure to compress and seal the synthesis plate 13, saves gas consumption, can achieve very good sealing effect at the same time, is more suitable for accurately controlling the conditions of reagent reaction and waste liquid discharge in a plurality of synthesis columns on a synthesizer by two mutually noninterfere compression and blowing air inlet channels, has simple structure, convenient assembly and simple disassembly, has low restriction on instrument structure, is suitable for DNA synthesizers with various structure types, and greatly reduces maintenance difficulty and cost.
Drawings
FIG. 1 is a schematic view of a compacting, sealing, air-blowing and liquid-draining device for a DNA synthesizer according to the present invention;
FIG. 2 is a schematic view of a silica gel fixing plate used on a compacting, sealing, air blowing and draining device on a DNA synthesizer;
FIG. 3 is a schematic view of an upper cylinder of a compression seal blow-down liquid discharge device for use in a DNA synthesizer according to the present invention;
FIG. 4 is an exploded view of a compression seal blow-down liquid discharge device for a DNA synthesizer according to the present invention.
In the drawings, the list of components represented by the various numbers is as follows:
1. cylinder, 2, piston assembly, 2.1 middle perspective plate, 2.2, rubber sealing ring, 2.3, hollow groove, 2.4, annular groove, 2.5, silica gel fixing plate, 2.6, silica gel pad, 3, first through hole, 4, second through hole, 5, elastic fixing assembly, 5.1, spring fixing bracket, 5.2, L-shaped groove, 5.3, spring, 5.4 screw, 6, upper perspective plate, 7, connecting part, 7.1, hose, 7.2, screw connector, 7.3, screw elbow, 8, semi-annular groove body, 9, straight-through screw quick connector, 10, reducing quick connector, 11, air outlet, 12, screw blind hole, 13, synthetic plate
Detailed Description
The principles and features of the present invention are described below with reference to the drawings, the examples are illustrated for the purpose of illustrating the invention and are not to be construed as limiting the scope of the invention.
In the description of the present invention, it should be understood that the terms "upper," "lower," "center," "inner," "outer," "top," "bottom," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the invention.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
As shown in fig. 1-4, the invention provides a compacting, sealing and blowing liquid discharging device for a DNA synthesizer, which comprises a cylinder body 1 and a piston assembly 2, wherein the piston assembly 2 is slidably arranged in the cylinder body 1, a first cavity is formed in the upper part of the piston assembly 2 and the cylinder body 1, the outer side surface of the piston assembly 2 is in sealing connection with the inner surface of the cylinder body 1, a first through hole 3 and a second through hole 4 are formed in the cylinder body 1, the first through hole 3 is communicated with the first cavity, a third through hole is formed in the inner side of the piston assembly 2, and the second through hole 4 is communicated with the third through hole. Thus, the piston assembly 2 is slidably arranged inside the cylinder 1, a first cavity is formed between the upper part of the piston assembly 2 and the inside of the cylinder 1, when the first cavity is inflated through the first through hole 3 formed in the cylinder 1, the outer side surface of the piston assembly 2 is in sealing connection with the inner surface of the cylinder 1, so that the piston assembly 2 is forced to move downwards due to the fact that the second cavity can generate great gas pressure, the synthetic plate 13 on the DNA synthesizer is further compressed, a second cavity is formed between the piston assembly 2 and the synthetic plate 13 on the DNA synthesizer, at the moment, the second cavity is inflated through the second through hole 4 formed in the cylinder 1, the communication between the second through hole 4 and the second cavity is realized through the third through hole, the third through hole is formed on the inner side surface of the piston assembly 2, and the third through hole is communicated with the second through hole 4. Therefore, the device has compact structure and small size, can generate great pressure under very small pressure to compress and seal the target, saves gas consumption, can achieve very good sealing effect simultaneously, is more suitable for accurately controlling the conditions of reagent reaction and waste liquid discharge in a plurality of synthesis columns on a synthesizer by two mutually noninterfere compressing and blowing air inlet channels, has simple structure, convenient assembly and simple disassembly, has low restriction on instrument structure, is suitable for being used by DNA synthesizers with various structure types, and greatly reduces maintenance difficulty and cost.
The point to be described here is: the cylinder body 1 and the piston assembly 2 can be arranged into square structures, so that the stress area is increased, the unnecessary space is not occupied, the large thrust can be generated under certain pressure, the whole device is compact in structure, and the space is saved.
Here, a point to be described is also: the cylinder body 1 can be provided with a left cavity and a right cavity to form a left sealed cavity and a right sealed cavity, and then the piston assemblies 2 with corresponding numbers are arranged to meet the requirements of different synthetic column numbers of the synthetic plates 13.
The invention relates to a compacting, sealing, blowing and draining device for a DNA synthesizer, which can be based on the technical proposal described above as shown in figures 1-4: the cylinder body is characterized by further comprising an elastic fixing assembly 5, wherein the edge of the elastic fixing assembly 5 is fixedly connected with the lower portion of the cylinder body 1, and the elastic fixing assembly 5 is elastically connected with the lower surface of the piston assembly 2. Thus, when the first through hole 3 is used as the air outlet for pressure relief, the elastic fixing component 5 is elastically connected with the lower surface of the piston component 2, and the piston component 2 can be reset under the elastic action of the elastic fixing component 5 so as to facilitate the next compression air blowing.
The invention relates to a compacting, sealing, blowing and draining device for a DNA synthesizer, which can be based on the technical proposal described above as shown in figures 1-4: the elastic fixing assembly 5 comprises a spring fixing support 5.1 and a plurality of springs 5.3, wherein the spring fixing support 5.1 is provided with L-shaped grooves 5.2, the springs 5.3 are uniformly distributed inside the L-shaped grooves 5.2, the springs 5.3 are fixedly connected with the spring fixing support 5.1, the upper ends of the springs 5.3 are propped against the lower surface of the piston assembly 2, and the spring fixing support 5.1 is fixedly connected with the lower portion of the cylinder body 1. Like this, spring 5.3 evenly distributed is inside L type cell body, and spring 5.3's lower extreme and spring fixed bolster 5.1 fixed connection, and spring 5.3's upper end offsets with piston assembly 2's lower surface, can make piston assembly 2 atress even, guarantees that piston assembly 2 resumes the normal position, simultaneously, spring fixed bolster 5.1's edge and cylinder body 1 lower part fixed connection also can play the effect of protection cylinder body 1.
The point to be described here is: the spring fixing support 5.1 can be further provided with screws 5.4 or supporting rods, the number of the screws is the same as that of the springs 5.3, the height of the screws 5.4 or the supporting rods is lower than that of the springs 5.3 when the springs 5.3 are stretched, the springs 5.3 are sleeved on the screws 5.4 or the supporting rods, and therefore the screws 5.4 or the supporting rods can play a role of guiding the springs 5.3, and then the piston assembly 2 is reset.
The invention relates to a compacting, sealing, blowing and draining device for a DNA synthesizer, which can be based on the technical proposal described above as shown in figures 1-4: the upper part of the cylinder body 1 is provided with a through hole, and an upper perspective plate 6 is arranged at the position of the through hole in a sealing way. Like this, the through-hole on cylinder body 1 upper portion sets up perspective board 6, and the setting of going up perspective board 6 can be convenient for observe the inside condition of cylinder body 1 to perspective board's side and through-hole viscidity sealing connection, viscidity sealing connection can guarantee the seal of first cavity.
The invention relates to a compacting, sealing, blowing and draining device for a DNA synthesizer, which can be based on the technical proposal described above as shown in figures 1-4: the piston assembly 2 comprises a middle perspective plate 2.1, a rubber sealing ring 2.2, a silica gel fixing plate 2.5 and a silica gel pad 2.6, wherein an annular groove 2.4 is formed in the outer side of the silica gel fixing plate 2.5, the rubber sealing ring 2.2 is sleeved inside the annular groove 2.4, a hollow groove 2.3 is formed in the middle of the silica gel fixing plate 2.5, the middle perspective plate (2.1) is arranged at the position of the hollow groove (2.3) in a sealing mode, and the silica gel pad 2.6 is fixed on the lower surface of the silica gel fixing plate 2.5. Therefore, the rubber sealing ring 2.2 is sleeved in the annular groove 2.4 at the outer side of the silica gel fixing plate 2.5, lubricating grease can be smeared at the outer side of the rubber sealing ring 2.2 so as to ensure that the piston assembly 2 can slide in the cylinder body 1, and meanwhile, the tightness of the cavity can be ensured; the middle perspective plate 2.1 is adhered to a hollow groove 2.3 formed in the middle of the silica gel fixing plate 2.5, and the arrangement of the middle perspective plate 2.1 and the upper perspective plate 6 can enable the whole compaction sealing device to conveniently observe the reaction condition of each reagent of the synthetic column on the compacted synthetic plate 13 and the discharge condition of waste liquid in the synthetic column; the silica gel pad 2.6 is fixed to be set up at the lower surface of silica gel fixed plate 2.5, and when first through-hole 3 was inflated to first cavity, silica gel fixed plate 2.5 downwardly moving compressed composite board 13, and silica gel pad 2.6 that silica gel fixed plate 2.5 lower surface set up can seal composite board 13.
The invention relates to a compacting, sealing, blowing and draining device for a DNA synthesizer, which can be based on the technical proposal described above as shown in figures 1-4: the second through hole 4 and the third through hole are communicated through a connecting component 7, a semi-annular groove body 8 is formed in the bottom of the cylinder body 1, the connecting component 7 is arranged in the semi-annular groove body 8, one end of the connecting component 7 is in sealing connection with the second through hole 4, and the other end of the connecting component 7 is in sealing connection with the third through hole. In this way, the connecting part 7 is placed in the semi-annular groove 8 formed at the bottom of the cylinder body 1, two ends of the connecting part 7 are respectively and hermetically connected with the second through hole 4 and the third through hole and are communicated with the second through hole 4 and the third through hole, and the connecting part 7 can guide the gas filled from the second through hole 4 into the second cavity formed by the lower part of the piston assembly 2 and the synthetic plate 13 so as to pressurize and charge the synthetic column on the synthetic plate 13.
The invention relates to a compacting, sealing, blowing and draining device for a DNA synthesizer, which can be based on the technical proposal described above as shown in figures 1-4: the connecting part 7 comprises a hose 7.1, a threaded connector 7.2 and a threaded elbow 7.3, wherein the threaded connector 7.2 and the threaded elbow 7.3 are of hollow structures, one end of the hose 7.1 is in sealing connection with the second through hole 4, the other end of the hose 7.1 is inserted into the threaded connector 7.2 in an interference manner and is in sealing connection with the threaded connector 7.2, the threaded connector 7.2 is in threaded connection with the threaded elbow 7.3, and the threaded elbow 7.3 is in sealing connection with the third through hole. Like this, second through-hole 4 and hose 7.1, hose 7.1 and threaded connection head 7.2, threaded connection head 7.2 and threaded elbow 7.3, threaded elbow 7.3 and third through-hole are sealing connection to threaded connection head 7.2 and threaded elbow 7.3 are hollow structure, can walk out, consequently, form an independent inlet channel from second through-hole 4 to the inboard third through-hole of silica gel fixed plate 2.5, can blow to the synthetic plate 13 that compresses tightly.
The invention relates to a compacting, sealing, blowing and draining device for a DNA synthesizer, which can be based on the technical proposal described above as shown in figures 1-4: the quick connector comprises a first through hole 3, a first through hole 3 and a second through hole 4, and is characterized by further comprising a through thread quick connector 9 and a reducing quick connector 10, wherein the through thread quick connector 9 is arranged on the first through hole 3 and is in threaded connection with the first through hole 3, and the reducing quick connector 10 is arranged on the second through hole 4 and is in threaded connection with the second through hole 4. Like this, straight-through screw quick connector 9 and reducing quick connector 10 respectively with first through-hole 3 and second through-hole 4 threaded connection, outside air supply can be through straight-through screw quick connector 9 to first through-hole 3 aerify, drive silica gel fixed plate 2.5 and push out and then compress tightly synthetic board 13 on the synthesizer, make silica gel fixed plate 2.5 lower part and synthetic board 13 the second cavity formation compress tightly sealing system, at this moment, outside air supply is through reducing quick connector 10 to second through-hole 4 aerify, the gas passes through connecting component 7 and third through-hole gets into the second cavity, blow to compressing tightly sealed synthetic board 13, and then realize the control to each reagent reaction degree and the exhaust liquid condition in the synthetic post.
The point to be described here is: the variable-diameter quick connector 10 can be a Y-shaped variable-diameter quick connector 10, two connectors of the Y-shaped variable-diameter quick connector 10 can be respectively connected with a connecting part 7, and the other ends of the connecting parts 7 are respectively connected with third through holes in a sealing mode.
The invention relates to a compacting, sealing, blowing and draining device for a DNA synthesizer, which can be based on the technical proposal described above as shown in figures 1-4: the third through hole is composed of an air outlet 11 and a threaded blind hole 12, the air outlet 11 is formed in the inner side face of the silica gel fixing plate 2.5, the threaded blind hole 12 is formed in the top face of the silica gel fixing plate 2.5, and the air outlet 11 is communicated with the threaded blind hole 12. Like this, gas outlet 11 is offered at the medial surface of silica gel fixed plate 2.5 to be located the lower part of silica gel fixed plate 2.5 cavity recess 2.3, screw thread blind hole 12 is offered in the position that silica gel fixed plate 2.5 top surface corresponds with gas outlet 11, and gas outlet 11 is linked together with screw thread blind hole 12, when aerifing to second through-hole 4, and gas can get into the second cavity that has compressed tightly sealedly through connecting component 7 and the third through-hole that comprises gas outlet 11 and screw thread blind hole 12, blows for the synthetic column.
The point to be described here is: when the reducing quick connector 10 is the Y-shaped reducing quick connector 10, two third through holes are correspondingly arranged on the silica gel fixing plate 2.5 according to the length of the connecting part 7, so that the compressed and sealed second cavity is inflated through the two passages, the inflation amount can be increased, and the working efficiency is improved.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (7)
1. A compress tightly sealed blowing flowing back device for on DNA synthesizer, its characterized in that: the novel piston assembly comprises a cylinder body (1) and a piston assembly (2), wherein the piston assembly (2) is arranged inside the cylinder body (1) in a sliding manner, a first cavity is formed in the upper portion of the piston assembly (2) and the cylinder body (1), the outer side face of the piston assembly (2) is in sealing connection with the inner surface of the cylinder body (1), a first through hole (3) and a second through hole (4) are formed in the cylinder body (1), the first through hole (3) is communicated with the first cavity, a third through hole is formed in the inner side of the piston assembly (2), and the second through hole (4) is communicated with the third through hole;
the upper part of the cylinder body (1) is provided with a through hole, and an upper perspective plate (6) is arranged at the position of the through hole in a sealing way;
the piston assembly (2) comprises a middle perspective plate (2.1), a rubber sealing ring (2.2), a silica gel fixing plate (2.5) and a silica gel pad (2.6), wherein an annular groove (2.4) is formed in the outer side of the silica gel fixing plate (2.5), the rubber sealing ring (2.2) is sleeved in the annular groove (2.4), a hollow groove (2.3) is formed in the middle part of the silica gel fixing plate (2.5), the middle perspective plate (2.1) is arranged at the position of the hollow groove (2.3) in a sealing mode, and the silica gel pad (2.6) is fixed on the lower surface of the silica gel fixing plate (2.5);
the cylinder body (1) comprises a left sealing cavity and a right sealing cavity, and each cylinder body (1) is correspondingly provided with a piston assembly (2).
2. The compacting, sealing, air-blowing and draining device for a DNA synthesizer according to claim 1, wherein: the cylinder body is characterized by further comprising an elastic fixing assembly (5), wherein the edge of the elastic fixing assembly (5) is fixedly connected with the lower portion of the cylinder body (1), and the elastic fixing assembly (5) is elastically connected with the lower surface of the piston assembly (2).
3. The compression seal blow-down liquid discharge device for a DNA synthesizer according to claim 2, wherein: the elastic fixing assembly (5) comprises a spring fixing support (5.1) and a spring (5.3), wherein the spring fixing support (5.1) is provided with an L-shaped groove (5.2), the spring (5.3) is evenly distributed inside the L-shaped groove (5.2), the spring (5.3) is fixedly connected with the spring fixing support (5.1), the upper end of the spring (5.3) abuts against the lower surface of the piston assembly (2), and the spring fixing support (5.1) is fixedly connected with the lower portion of the cylinder body (1).
4. A compression seal blow-down liquid discharge apparatus for use in a DNA synthesizer according to claim 3, wherein: the second through hole (4) and the third through hole are communicated through a connecting component (7), a semi-annular groove body (8) is formed in the bottom of the cylinder body (1), the connecting component (7) is arranged in the semi-annular groove body (8), one end of the connecting component (7) is in sealing connection with the second through hole (4), and the other end of the connecting component (7) is in sealing connection with the third through hole.
5. The compacting and sealing blow-down liquid discharge device for a DNA synthesizer according to claim 4, wherein: the connecting component (7) comprises a hose (7.1), a threaded connector (7.2) and a threaded elbow (7.3), wherein the threaded connector (7.2) and the threaded elbow (7.3) are of hollow structures, one end of the hose (7.1) is in sealing connection with the second through hole (4), the other end of the hose (7.1) is in interference insertion with the threaded connector (7.2) and in sealing connection with the threaded connector (7.2), the threaded connector (7.2) is in threaded connection with the threaded elbow (7.3), and the threaded elbow (7.3) is in sealing connection with the third through hole.
6. The compacting and sealing blow-down liquid discharge device for a DNA synthesizer according to claim 5, wherein: the novel quick connector further comprises a straight-through threaded quick connector (9) and a reducing quick connector (10), wherein the straight-through threaded quick connector (9) is arranged on the first through hole (3) and is in threaded connection with the first through hole (3), and the reducing quick connector (10) is arranged on the second through hole (4) and is in threaded connection with the second through hole (4).
7. The compacting and sealing blow-down liquid discharge device for a DNA synthesizer according to claim 6, wherein: the third through hole is composed of an air outlet (11) and a threaded blind hole (12), the air outlet (11) is formed in the inner side face of the silica gel fixing plate (2.5), the threaded blind hole (12) is formed in the top face of the silica gel fixing plate (2.5), and the air outlet (11) is communicated with the threaded blind hole (12).
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| CN201910776414.6A CN110408518B (en) | 2019-08-22 | 2019-08-22 | A compress tightly sealed flowing back device that blows for on DNA synthesizer |
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| CN201910776414.6A CN110408518B (en) | 2019-08-22 | 2019-08-22 | A compress tightly sealed flowing back device that blows for on DNA synthesizer |
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| CN110408518B true CN110408518B (en) | 2023-06-27 |
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| CN204529830U (en) * | 2014-11-03 | 2015-08-05 | 北京四环科学仪器厂有限公司 | A kind of pressurization pumping equipment being applied to DNA synthesizer |
| JP2017035117A (en) * | 2016-11-22 | 2017-02-16 | 株式会社日立製作所 | Two-dimensional cell array device and apparatus for gene quantification/sequence analysis |
| CN208410496U (en) * | 2018-05-18 | 2019-01-22 | 晋江市华昌机械工贸有限公司 | A kind of hydraulic foaming mould frame in high-pressure polyurethane foaming production line |
| CN210765270U (en) * | 2019-08-22 | 2020-06-16 | 北京擎科生物科技有限公司 | A compress tightly sealed flowing back that blows for on DNA synthesizer |
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2019
- 2019-08-22 CN CN201910776414.6A patent/CN110408518B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0804625D0 (en) * | 2007-10-19 | 2008-04-16 | Hsieh Kuo Chin | Vacuum fresh-keeping cover |
| CN204529830U (en) * | 2014-11-03 | 2015-08-05 | 北京四环科学仪器厂有限公司 | A kind of pressurization pumping equipment being applied to DNA synthesizer |
| JP2017035117A (en) * | 2016-11-22 | 2017-02-16 | 株式会社日立製作所 | Two-dimensional cell array device and apparatus for gene quantification/sequence analysis |
| CN208410496U (en) * | 2018-05-18 | 2019-01-22 | 晋江市华昌机械工贸有限公司 | A kind of hydraulic foaming mould frame in high-pressure polyurethane foaming production line |
| CN210765270U (en) * | 2019-08-22 | 2020-06-16 | 北京擎科生物科技有限公司 | A compress tightly sealed flowing back that blows for on DNA synthesizer |
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Address after: 100176 Floor 6, West Half Unit, Building 3, Yard 105, Jinghai 3rd Road, Daxing District, Beijing Economic-Technological Development Area Patentee after: Beijing Qingke Biotechnology Co.,Ltd. Address before: Building 5, Building 156, Jinghai Industrial Park, Daxing District Economic Development Zone, Beijing, 100176 Patentee before: Beijing Qingke Biotechnology Co.,Ltd. |