CN219991530U - Continuous split charging machine for nucleic acid extraction reagent - Google Patents
Continuous split charging machine for nucleic acid extraction reagent Download PDFInfo
- Publication number
- CN219991530U CN219991530U CN202320599014.4U CN202320599014U CN219991530U CN 219991530 U CN219991530 U CN 219991530U CN 202320599014 U CN202320599014 U CN 202320599014U CN 219991530 U CN219991530 U CN 219991530U
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- nucleic acid
- plate
- acid extraction
- extraction reagent
- reagent
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 51
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 29
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 29
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 29
- 238000000605 extraction Methods 0.000 title claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 56
- 238000007789 sealing Methods 0.000 claims description 17
- 230000005540 biological transmission Effects 0.000 claims description 10
- 238000009434 installation Methods 0.000 claims description 7
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 3
- 239000011148 porous material Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 210000002445 nipple Anatomy 0.000 description 3
- 238000004806 packaging method and process Methods 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a continuous split charging machine for nucleic acid extraction reagents, which relates to the technical field of nucleic acid detection, and particularly relates to a continuous split charging machine for nucleic acid extraction reagents. This continuous racking machine of nucleic acid extraction reagent is through setting up the confession liquid case to set up a plurality of burette in confession liquid case bottom, set up the piston board simultaneously at confession liquid incasement portion, drive the piston board through the second cylinder and remove, can release the reagent in the confession liquid case and discharge along the burette and split charging, the confession liquid incasement volume is great, need not add the reagent frequently, thereby sustainable split charging to the reagent, improves split charging efficiency.
Description
Technical Field
The utility model relates to the technical field of nucleic acid detection, in particular to a continuous split charging machine for nucleic acid extraction reagents.
Background
The nucleic acid extraction reagent has the function of extracting nucleic acid in cells, common reagents for nucleic acid extraction include guanidine isothiocyanate, guanidine hydrochloride, sarcosyl and the like, and the principle of each reagent in the nucleic acid extraction process is different, but the use method is to add the reagent into a detection sample and sufficiently react and then further purify the sample.
For the process of sub-packaging nucleic acid sample test tubes after reagent is extracted from nucleic acid, patent number CN202122195153.4 discloses a continuous sub-packaging machine for nucleic acid extraction reagent, wherein a lifting cylinder, a floating plate, a dropper cylinder, a dropper assembly, an orifice plate and sample test tubes are arranged, a unit bottom end, a rubber nipple and a dropper pressing plate are arranged in the dropper assembly, the dropper pressing plate is driven to move through the dropper cylinder, the rubber nipple is extruded, so that the reagent in the unit dropper is sub-packaged into the sample test tubes in the orifice plate, a large number of sample test tubes can be sub-packaged synchronously, but the reagent is sucked and sub-packaged through the combination of the unit dropper and the rubber nipple, the reagent sucking operation is required to be frequently performed on the unit dropper in the operation process, the sub-packaging efficiency is reduced, and only one group of orifice plates are arranged.
Disclosure of Invention
Aiming at the defects of the prior art, the utility model provides a continuous split charging machine for nucleic acid extraction reagents, and solves the problems in the background art.
In order to achieve the above purpose, the utility model is realized by the following technical scheme: the utility model provides a nucleic acid extraction reagent divides installation in succession, includes the racking machine bottom plate, four guide posts of top fixed mounting of racking machine bottom plate, and the top fixed mounting of guide post has the installation roof, the top fixed mounting of installation roof has first cylinder, and fixedly connected with drive plate on the output shaft of first cylinder, the bottom of drive plate is provided with the confession liquid case, and the inside joint of confession liquid case has the piston board, the bottom threaded connection of confession liquid case has a plurality of burette, the top fixed mounting of drive plate has two sets of second cylinders, and the output shaft and the piston board fixed connection of second cylinder, the top movable mounting of racking machine bottom plate has the regulation slip table, and adjusts the top fixed mounting of slip table and have two sets of mounting hole plates, the top joint of mounting hole plate has a plurality of sample test tubes.
Preferably, the transmission plate is movably sleeved outside the guide post, a sealing top plate is fixedly arranged at the bottom of the transmission plate, and the sealing top plate is fixedly connected with the liquid supply box.
Preferably, a sealing ring is glued on the peripheral side of the piston plate, and the outer ring of the sealing ring abuts against the inner wall of the liquid supply tank.
Preferably, the liquid injection valve is fixedly connected to the lower part of the front face of the liquid supply box, and the whole liquid supply box is made of acrylic materials.
Preferably, the number of the droppers is the same as that of the sample test tubes clamped on the group of mounting hole plates, and the droppers correspond to the positions of the sample test tubes one by one.
Preferably, the top fixed mounting of racking machine bottom plate has the direction slide rail, adjust the bottom fixed mounting of slip table have with direction slide rail complex direction sliding sleeve, adjust the slip table through direction slide rail, direction sliding sleeve movable mounting in racking machine bottom plate top.
Preferably, both ends of the adjusting sliding table are connected with locking knobs in a threaded manner, and threaded holes matched with the locking knobs are formed in the top of the bottom plate of the split charging machine.
The utility model provides a nucleic acid extraction reagent continuous racking machine, which has the following beneficial effects:
1. this continuous racking machine of nucleic acid extraction reagent sets up the confession liquid case to set up a plurality of burette in confession liquid case bottom, set up the piston board simultaneously at confession liquid incasement portion, drive the piston board through the second cylinder and remove, can release the reagent in the confession liquid case and discharge along the burette and split into, confession liquid incasement volume is great, need not add the reagent frequently, thereby sustainable split into the reagent, improves split charging efficiency, and sets up the notes liquid valve on the confession liquid case, be convenient for add the reagent to confession liquid incasement portion.
2. This continuous racking machine of nucleic acid extraction reagent sets up mobilizable regulation slip table through at racking machine bottom plate top to set up two sets of mounting hole boards at regulation slip table top, can adjust two sets of mounting hole board positions in turn, when sample test tube in a set of mounting hole board carries out the racking reagent, can load and unload sample test tube on another set of mounting hole board, thereby can realize carrying out the partial shipment to reagent in succession, further improves partial shipment efficiency.
Drawings
FIG. 1 is a schematic diagram of the overall assembled structure of the present utility model;
FIG. 2 is a schematic view of a cross-section of a liquid supply tank according to the present utility model;
FIG. 3 is a schematic view of the structure of the inside of the liquid supply tank of the present utility model;
FIG. 4 is a schematic view of the top of the adjusting slipway of the present utility model;
fig. 5 is a schematic structural diagram of the adjustment sliding table and the bottom plate of the split charging machine.
In the figure: 1. a bottom plate of the split charging machine; 2. adjusting the sliding table; 3. a guide rail; 4. a guide sliding sleeve; 5. installing an orifice plate; 6. a sample tube; 7. a locking knob; 8. a guide post; 9. installing a top plate; 10. a first cylinder; 11. a drive plate; 12. a second cylinder; 13. sealing the top plate; 14. a liquid supply tank; 15. a piston plate; 16. a seal ring; 17. a liquid injection valve; 18. a dropper.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
Referring to fig. 1 and 2, the present utility model provides the following technical solutions: the continuous split charging machine for extracting the nucleic acid comprises a split charging machine bottom plate 1, four groups of guide posts 8 are fixedly arranged at the top of the split charging machine bottom plate 1, a mounting top plate 9 is fixedly arranged at the top of the guide posts 8, a first air cylinder 10 is fixedly arranged at the top of the mounting top plate 9, a transmission plate 11 is fixedly connected to an output shaft of the first air cylinder 10, a liquid supply tank 14 is arranged at the bottom of the transmission plate 11, a piston plate 15 is clamped in the liquid supply tank 14, a plurality of droppers 18 are connected at the bottom of the liquid supply tank 14 in a threaded manner, two groups of second air cylinders 12 are fixedly arranged at the top of the transmission plate 11, the output shaft of the second air cylinders 12 is fixedly connected with the piston plate 15, the piston plate 15 is driven to move through the second air cylinders 12, the reagent in the liquid supply tank 14 can be pushed out and discharged along the droppers 18 for split charging, the internal volume of the liquid supply tank 14 is large, and the reagent does not need to be added frequently, the top of the bottom plate 1 of the split charging machine is movably provided with an adjusting sliding table 2, the top of the adjusting sliding table 2 is fixedly provided with two groups of mounting pore plates 5, the positions of the two groups of mounting pore plates 5 can be alternately adjusted, when sample test tubes 6 in one group of mounting pore plates 5 are used for split charging reagents, the other group of mounting pore plates 5 can be used for loading and unloading the sample test tubes 6, thus reagents can be continuously split charged, the top of the mounting pore plates 5 is clamped with a plurality of sample test tubes 6, a plurality of sample test tubes 6 can be synchronously split charged into a large number of sample test tubes 6 through a dropper 18, the split charging efficiency is improved, a transmission plate 11 is movably sleeved outside a guide post 8, the bottom of the transmission plate 11 is fixedly provided with a sealing top plate 13, the transmission plate 11, the sealing top plate 13 and a liquid supply box 14 are connected through bolts, the firmness after installation is ensured, the disassembly is convenient, the sealing top plate 13 is fixedly connected with the liquid supply box 14, the top sealing plate 13 seals the top of the liquid supply tank 14.
Referring to fig. 2 and 3, a sealing ring 16 is glued on the peripheral side of the piston plate 15, the outer ring of the sealing ring 16 abuts against the inner wall of the liquid supply tank 14, the sealing performance of the contact surface between the piston plate 15 and the inner wall of the liquid supply tank 14 is guaranteed by arranging the sealing ring 16 on the peripheral side of the piston plate 15, the liquid supply tank 14 can squeeze reagents, a liquid injection valve 17 is fixedly connected to the lower side of the front face of the liquid supply tank 14, reagents are conveniently added into the liquid supply tank 14, the whole liquid supply tank 14 is made of acrylic materials, the whole liquid supply tank 14 is transparent, the reagents in the liquid supply tank 14 are conveniently observed, the number of droppers 18 is the same as the number of the sample test tubes 6 clamped on the group of mounting hole plates 5, the droppers 18 correspond to the positions of the sample test tubes 6 one by one, and the reagent can be synchronously split into a large number of sample test tubes 6 through the droppers 18, so that the split charging efficiency is improved.
Referring to fig. 4 and 5, the top of the bottom plate 1 of the racking machine is fixedly provided with a guide sliding rail 3, the bottom of the adjusting sliding table 2 is fixedly provided with a guide sliding sleeve 4 matched with the guide sliding rail 3, the adjusting sliding table 2 is movably mounted at the top of the bottom plate 1 of the racking machine through the guide sliding rail 3 and the guide sliding sleeve 4, the adjusting sliding table 2 is limited through the guide sliding rail 3 and the guide sliding sleeve 4, the stability of the adjusting sliding table 2 during moving is ensured, two ends of the adjusting sliding table 2 are connected with locking knobs 7 in a threaded manner, the top of the bottom plate 1 of the racking machine is provided with threaded holes matched with the locking knobs 7, after a group of mounting pore plates 5 are adjusted in place, the corresponding locking knobs 7 can be rotated, the adjusting sliding table 2 and the mounting pore plates 5 are locked, and the sample test tubes 6 on the mounting pore plates 5 are ensured to be accurately corresponding to the droppers 18.
In summary, this nucleic acid extraction reagent continuous racking machine, when in use, with sample test tube 6 joint in the joint hole at mounting orifice 5 top, push the mounting orifice 5 of clamping sample test tube 6 to the confession liquid case 14 bottom, and rotate locking knob 7 and will adjust slip table 2, mounting orifice 5 locking, inject the reagent to the inside of confession liquid case 14 along annotating liquid valve 17 through instrument such as needle tubing, start first cylinder 10 and drive plate 11 and confession liquid case 14 and move down, adjust the burette 18 position, card burette 18 into corresponding sample test tube 6, start second cylinder 12 and drive piston plate 15 and remove, extrude the reagent through piston plate 15, with the inside reagent of confession liquid case 14 along burette 18, realize the partial shipment to the reagent, after the partial shipment of sample test tube 6 on a set of mounting orifice 5, follow the confession liquid case 14 bottom and push out another set of mounting orifice 5 put in place, two sets of mounting orifice 5 alternate use, when the sample test tube 6 in the mounting orifice 5 carries out the reagent to another set of mounting orifice 5, carry out continuous loading and unloading to the sample test tube 6, can realize the continuous loading and unloading to the reagent.
In the description of the present utility model, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are based on directions or positional relationships shown in the drawings, are merely for convenience of description and simplification of description, and do not indicate or imply that the apparatus or element to be referred to must have a specific direction, be constructed and operated in the specific direction, and thus should not be construed as limiting the present utility model; the terms "first," "second," "third," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "coupled," and the like are to be construed broadly, and may be fixedly coupled, detachably coupled, or integrally coupled, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. Nucleic acid extraction reagent divides installation in succession, including racking machine bottom plate (1), four guide posts (8) of top fixed mounting of racking machine bottom plate (1), and the top fixed mounting of guide post (8) has installation roof (9), the top fixed mounting of installation roof (9) has first cylinder (10), and fixedly connected with drive plate (11) on the output shaft of first cylinder (10), its characterized in that: the bottom of drive plate (11) is provided with confession liquid case (14), and the inside joint of confession liquid case (14) has piston board (15), the bottom threaded connection of confession liquid case (14) has a plurality of burette (18), the top fixed mounting of drive plate (11) has two sets of second cylinders (12), and the output shaft and the piston board (15) fixed connection of second cylinder (12), the top movable mounting of racking machine bottom plate (1) has regulation slip table (2), and the top fixed mounting who adjusts slip table (2) has two sets of mounting hole plates (5), the top joint of mounting hole plates (5) has a plurality of sample test tubes (6).
2. The nucleic acid extraction reagent continuous dispensing machine according to claim 1, wherein: the transmission plate (11) is movably sleeved outside the guide column (8), a sealing top plate (13) is fixedly arranged at the bottom of the transmission plate (11), and the sealing top plate (13) is fixedly connected with the liquid supply box (14).
3. The nucleic acid extraction reagent continuous dispensing machine according to claim 1, wherein: the periphery of the piston plate (15) is glued with a sealing ring (16), and the outer ring of the sealing ring (16) is propped against the inner wall of the liquid supply box (14).
4. The nucleic acid extraction reagent continuous dispensing machine according to claim 1, wherein: the front lower part of the liquid supply box (14) is fixedly connected with a liquid injection valve (17), and the whole liquid supply box (14) is made of acrylic materials.
5. The nucleic acid extraction reagent continuous dispensing machine according to claim 1, wherein: the number of the droppers (18) is the same as that of the sample tubes (6) clamped on the group of mounting hole plates (5), and the droppers (18) are in one-to-one correspondence with the positions of the sample tubes (6).
6. The nucleic acid extraction reagent continuous dispensing machine according to claim 1, wherein: the top fixed mounting of racking machine bottom plate (1) has direction slide rail (3), the bottom fixed mounting of adjusting slip table (2) has with direction slide rail (3) complex direction sliding sleeve (4), adjust slip table (2) through direction slide rail (3), direction sliding sleeve (4) movable mounting in racking machine bottom plate (1) top.
7. The nucleic acid extraction reagent continuous dispensing machine according to claim 1, wherein: the two ends of the adjusting sliding table (2) are connected with locking knobs (7) in a threaded mode, and threaded holes matched with the locking knobs (7) are formed in the top of the bottom plate (1) of the split charging machine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320599014.4U CN219991530U (en) | 2023-03-23 | 2023-03-23 | Continuous split charging machine for nucleic acid extraction reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320599014.4U CN219991530U (en) | 2023-03-23 | 2023-03-23 | Continuous split charging machine for nucleic acid extraction reagent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219991530U true CN219991530U (en) | 2023-11-10 |
Family
ID=88613461
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320599014.4U Active CN219991530U (en) | 2023-03-23 | 2023-03-23 | Continuous split charging machine for nucleic acid extraction reagent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219991530U (en) |
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2023
- 2023-03-23 CN CN202320599014.4U patent/CN219991530U/en active Active
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