CN220696791U - Reagent filling equipment - Google Patents

Abstract

The utility model discloses a reagent pouring device. The pouring reagent equipment comprises a pouring assembly, a first driving piece and a second driving piece, wherein the pouring assembly comprises a pouring needle head, the pouring assembly comprises a pouring platform, the pouring platform is used for placing a pouring plate, the first driving piece is arranged on the pouring platform and used for driving the pouring plate or the pouring needle head to move along a first direction, and the second driving piece is arranged on the pouring platform and used for driving the pouring needle head to move along a vertical direction. This reagent pouring equipment can make annotate liquid syringe needle in proper order with annotate every group on the liquid board annotate the liquid hole just to setting up, can make annotate liquid syringe needle business turn over corresponding annotate liquid hole through the second driving piece to accomplish the notes liquid operation to all annotating liquid holes on the liquid board, degree of automation is high, annotates liquid efficient, and has reduced artificial operation intensity.

Description

Reagent filling equipment

Technical Field

The utility model relates to the technical field of reagent pouring, in particular to reagent pouring equipment.

Background

Reagent liquid separation and pouring are common operation steps in gene experiments, and the prior art generally adopts manpower to perform liquid separation pouring, but the labor intensity of manually performing liquid separation pouring is high, the efficiency is low, and the use requirements of users cannot be met.

Therefore, how to provide a perfusion reagent device with high automation degree is a technical problem to be solved.

Disclosure of Invention

The utility model aims to provide a reagent pouring device which has high automation degree and high pipetting and pouring efficiency, and reduces the labor intensity.

To achieve the purpose, the utility model adopts the following technical scheme:

a perfusion reagent apparatus comprising: the liquid injection assembly comprises a liquid injection needle head for injecting liquid; the pouring assembly comprises a pouring platform, and the pouring platform is used for placing a liquid injection plate; the first driving piece is arranged on the pouring platform and is used for driving the liquid injection plate or the liquid injection needle to move along a first direction; the second driving piece is arranged on the pouring platform and is used for driving the liquid injection needle to move along the vertical direction.

Preferably, the liquid injection assembly further comprises a liquid injection pump, a liquid injection pipe and a switch valve, one end of the liquid injection pipe is communicated with a reagent bottle containing a reagent, the other end of the liquid injection pipe is communicated with the liquid injection needle, and the liquid injection pump and the switch valve are both arranged on the liquid injection pipe.

Preferably, the injection assembly comprises a plurality of injection needle heads arranged at intervals along the second direction.

Preferably, the pouring reagent device further comprises a lifting support, the lifting support comprises a vertical plate and a needle support, the output end of the second driving piece is connected with the vertical plate, the needle support is arranged above the pouring platform, and the liquid injection needle is arranged on the needle support.

Preferably, the pouring reagent device further comprises a supporting table, the supporting table is movably arranged on the pouring platform, the supporting table is used for supporting the pouring plate, the first driving piece is in transmission connection with the supporting table and used for driving the supporting table to move along the first direction.

Preferably, the perfusion reagent apparatus further comprises a guiding mechanism for guiding movement of the support table in the first direction.

Preferably, the first driving piece comprises a first driving motor, a driving wheel, a synchronous belt and a driven wheel, wherein the first driving motor is fixedly arranged on the pouring platform, a motor shaft of the first driving motor is connected with the driving wheel, the driving wheel and the driven wheel are arranged along the first direction at intervals, the driven wheel is rotationally connected on the pouring platform, the synchronous belt is sleeved on the driving wheel and the driven wheel, the synchronous belt is connected with the supporting table, and the synchronous belt drives the supporting table to synchronously move.

Preferably, the perfusion reagent device further comprises a waste liquid collecting member fixedly connected with the support table, and the waste liquid collecting member can move along the first direction along with the support table, and a waste liquid tank is arranged on the waste liquid collecting member.

Preferably, the perfusion reagent apparatus further comprises a waste liquid discharge pipe connected to one side of the waste liquid collecting member, and a liquid inlet end of the waste liquid discharge pipe is communicated with the waste liquid tank.

Preferably, the pouring reagent device further comprises a detection mechanism, wherein the detection mechanism is arranged on the pouring platform and is used for detecting whether the pouring plate is arranged on the pouring platform.

The utility model has the beneficial effects that:

the pouring reagent equipment comprises a pouring assembly, a first driving piece and a second driving piece, wherein the pouring assembly comprises a pouring needle head used for pouring liquid, the pouring assembly comprises a pouring platform, the pouring platform is used for placing a pouring plate, the first driving piece is arranged on the pouring platform and used for driving the pouring plate or the pouring needle head to move along a first direction, and the second driving piece is arranged on the pouring platform and used for driving the pouring needle head to move along a vertical direction. This reagent pouring equipment can make annotate liquid syringe needle in proper order with annotate every group on the liquid board annotate the liquid hole just to setting up, can make annotate liquid syringe needle business turn over corresponding annotate liquid hole through the second driving piece to accomplish the notes liquid operation to all annotating liquid holes on the liquid board, degree of automation is high, annotates liquid efficient, and has reduced artificial operation intensity.

Drawings

FIG. 1 is a schematic view of a perfusion reagent apparatus according to an embodiment of the present utility model at a certain viewing angle;

FIG. 2 is a top view of a perfusion reagent apparatus provided by an embodiment of the present utility model;

fig. 3 is a schematic view of a perfusion reagent apparatus according to an embodiment of the present utility model at another perspective.

In the figure:

100. a liquid injection assembly; 101. a liquid injection needle; 102. a liquid injection pump; 103. a switch valve;

200. a perfusion assembly; 201. a perfusion platform; 202. a liquid injection plate; 2021. a liquid injection hole; 203. a support table;

300. a first driving member;

400. a second driving member; 401. a first driving motor; 402. a driving wheel; 403. a synchronous belt; 404. driven wheel; 405. a connecting block;

500. a lifting bracket; 501. A vertical plate; 502. A needle holder;

600. a guide mechanism; 601. A guide rod; 602. A support plate;

700. a waste liquid collecting member; 701. A waste liquid tank;

800. a waste liquid discharge pipe;

900. a detection mechanism;

110. a controller;

120. and (3) a housing.

Detailed Description

The following description of the embodiments of the present utility model will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixed or removable, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

As shown in fig. 1 to 3, the present utility model provides a priming reagent apparatus, which can automatically perform the priming operation on all the priming holes 2021 on the priming plate 202, and does not need to be manually involved in the priming operation, and has high automation degree and high priming efficiency. It should be noted that, the liquid injection plate 202 may be a deep well plate, a PCR plate, or other plates, as long as it has a plurality of hole structures and has liquid injection requirements.

Specifically, the priming reagent device includes a priming assembly 100, a priming assembly 200, a first driver 300, and a second driver 400. The liquid injection assembly 100 is a main component for performing liquid injection operation, and the liquid injection assembly 100 comprises liquid injection needles 101 for injecting liquid, wherein the number of the liquid injection needles 101 can be one or a plurality of liquid injection needles, and the liquid injection needles can be flexibly set according to requirements. The pouring assembly 200 comprises a pouring platform 201, the pouring platform 201 is used for placing a pouring plate 202, the pouring platform 201 is a main installation structure of pouring reagent equipment, the pouring plate 202, a first driving piece 300 and a second driving piece 400 are all arranged on the pouring platform 201, the pouring plate 202 is arranged below a pouring needle 101, a plurality of groups of pouring holes 2021 are arranged on the pouring plate 202 at intervals along a first direction, each group of pouring holes 2021 comprises at least one pouring hole 2021, and the first direction is shown in fig. 2. The first driving member 300 is used for driving the injection plate 202 or the injection needle 101 to move along a first direction, so that the injection needle 101 is disposed opposite to each group of injection holes 2021 in sequence. The second driving member 400 is used for driving the injection needle 101 to move in the vertical direction.

In the injection operation, first, the first driving member 300 acts to realize that the injection needle 101 is disposed opposite to a certain group of injection holes 2021; then, the second driving member 400 is operated to move the priming needle 101 downward to extend into the priming hole 2021 for priming operation, and to move upward after priming is completed until the priming needle 101 moves out of the priming hole 2021; finally, the first driving member 300 moves again, so that the liquid injection needle 101 and the next group of liquid injection holes 2021 are opposite to each other, and the operation is repeated until the liquid injection holes 2021 on the liquid injection plate 202 are all injected with the reagent liquid, and the liquid injection operation of one liquid injection plate 202 is completed by the reagent injection device. Compared with manual liquid injection, the automatic liquid injection of the liquid injection plate 202 is realized by the liquid injection reagent equipment, repeated liquid absorption by manpower is not needed to be added into the liquid injection plate 202, the operation intensity of workers is reduced, the liquid injection efficiency is improved, and the error rate in the reagent transferring operation process is reduced.

It should be noted that, the first driving member 300 may drive the injection plate 202 to move, may drive the injection needle 101 to move, or may drive the injection plate 202 and the injection needle 101 to move in opposite directions at the same time, and may drive the injection plate 202 and the injection needle 101 to move in the same direction at different moving speeds, so long as the injection needle 101 can be disposed opposite to each group of injection holes 2021 in sequence.

With continued reference to FIG. 1, to enhance the stability of the support for the injection plate 202, the perfusion reagent apparatus further comprises a support table 203, the support table 203 being movably arranged on the perfusion platform 201, the support table 203 being for supporting the injection plate 202. In some embodiments, the support table 203 is provided with a receiving groove, and the bottom end of the injection plate 202 can be placed in the receiving groove, so as to improve the stability of the injection plate 202 on the pouring platform 201. In some embodiments, a fastening structure is provided on the support table 203, and the flange of the injection plate 202 can be fixed on the support table 203 through the fastening structure, so as to improve the stability of the injection plate 202 on the injection platform 201. It should be noted that, since the liquid injection plate 202 has a plurality of types, the pouring reagent device may include a plurality of support tables 203, and different support tables 203 may have different holding grooves or different arrangement buckle structures, so that the purpose of supporting different types of liquid injection plates 202 can be achieved by replacing different support tables 203, and thus the universality of the pouring reagent device is improved.

With continued reference to fig. 1, the first drive member 300 is drivingly connected to the support table 203 and is configured to drive the support table 203 in a first direction. In some embodiments, the first driving member 300 is a combination of a motor and a pulley mechanism, specifically, the first driving member 300 includes a first driving motor 401, a driving wheel 402, a synchronous belt 403 and a driven wheel 404, the first driving motor 401 may be a servo motor, the first driving motor 401 is fixedly disposed on the pouring platform 201, a motor shaft of the first driving motor 401 is connected with the driving wheel 402, the driving wheel 402 and the driven wheel 404 are disposed at intervals along a first direction, the driven wheel 404 is rotatably connected on the pouring platform 201, the synchronous belt 403 is sleeved on the driving wheel 402 and the driven wheel 404, and the synchronous belt 403 is fixedly connected with the supporting table 203.

In some embodiments, the timing belt 403 is directly fixedly connected to the side wall surface of the support table 203, in some embodiments, as shown in fig. 2, the timing belt 403 is fixedly connected to the support table 203 through a connection block 405, the connection block 405 is fixed on the side wall surface of the support table 203, a through hole is penetrating through the connection block 405 along the first direction, and the timing belt 403 is penetrating through the through hole and is fixedly connected to the connection block 405. Of course, in other embodiments, the first driving member 300 may also employ a combination of a motor and a screw nut mechanism; alternatively, the first driving member 300 may be a linear motor; alternatively, the first driver 300 may be an air cylinder, and any mechanism capable of outputting linear motion may be used as the first driver 300.

To improve the accuracy of the movement of the priming plate 202 in the first direction, with continued reference to fig. 1, the priming reagent device further comprises a guiding mechanism 600, the guiding mechanism 600 being adapted to provide guiding of the movement of the support table 203 in the first direction. In some embodiments, the guide mechanism 600 includes a guide rod 601, two ends of the guide rod 601 are fixed on the pouring platform 201 through a support plate 602, the guide rod 601 is disposed along the first direction, a guide hole is disposed through the support table 203, and the guide rod 601 is disposed through the guide hole. In some specific embodiments, the number of the guide rods 601 is plural, the plurality of guide rods 601 are arranged at intervals along the direction perpendicular to the first direction, and correspondingly, a plurality of guide holes are arranged on the supporting table 203, and the plurality of guide rods 601 are penetrated in a one-to-one correspondence manner with the plurality of guide holes.

In some other embodiments, the guide mechanism 600 includes a guide rail disposed on the perfusion platform 201 in the first direction and a guide block disposed on the support table 203, the guide block being slidably coupled to the guide rail.

In order to control the liquid injection amount to realize accurate liquid injection, and with continued reference to fig. 1, the liquid injection assembly 100 further comprises a liquid injection pump 102, a liquid injection pipe and a switch valve 103, the reagent bottle is used for containing reagent liquid required to be injected onto the liquid injection plate 202, one end of the liquid injection pipe is communicated with the reagent bottle, the other end of the liquid injection pipe is communicated with the liquid injection needle 101, the liquid injection pump 102 is fixedly connected onto the injection platform 201, and the liquid injection pump 102 and the switch valve 103 are both arranged on the liquid injection pipe. The infusion pump 102 powers the flow of reagent liquid from the reagent liquid, thereby enabling reagent liquid to be pumped from the reagent bottle into the infusion needle 101. The on-off valve 103 is used to control the on-off of the reagent flow path so that a predetermined amount of reagent can be injected into the injection hole 2021 of the injection plate 202 through the injection needle 101. In some embodiments, the on-off valve 103 is a two-position three-way electromagnetic valve, and reagent liquid extracted from the reagent bottle is uniformly poured onto the liquid injection plate 202 through a signal sent by the two-position three-way valve.

In some embodiments, each set of injection holes 2021 includes a plurality of injection holes 2021 spaced apart along a second direction, which is perpendicular to the first direction as shown in fig. 2, that is, the injection plate 202 has a plurality of reagent holes arranged in rows and columns. In order to improve the pouring efficiency, the pouring assembly 100 includes a plurality of pouring needles 101 arranged at intervals along the second direction, and the plurality of pouring needles 101 are arranged in one-to-one correspondence with the plurality of pouring holes 2021 in each group of pouring holes 2021, so that the pouring reagent device can complete pouring of one group of reagent holes at a time.

Of course, in other embodiments, the priming needle 101 may be provided in more than one number, thereby enabling the priming reagent device to complete priming of multiple sets of reagent wells at a time. For example, twelve sets of reagent wells are provided on the injection plate 202, each set of reagent wells including eight reagent wells, and then the number of injection needles 101 may be 1, 2, 3, 4, 6, or 12, with eight injection needles 101 per set.

With continued reference to fig. 1, the reagent pouring apparatus further includes a lifting support 500, where the lifting support 500 is made of a rigid material, the lifting support 500 includes a vertical plate 501 and a needle support 502, the output end of the second driving member 400 is connected to the vertical plate 501, the needle support 502 is disposed above the pouring platform 201, and the liquid pouring needle 101 is disposed on the needle support 502.

In some embodiments, the second driving part 400 is a combination of a motor and a rack and pinion mechanism, and in particular, the second driving part 400 includes a second driving motor, a gear, and a rack, a motor shaft of the second driving motor is connected to the gear, the rack is disposed on the vertical plate 501 in a vertical direction, and the rack can be driven to move up and down by rotation of the gear, so that the lifting bracket 500 can move up and down. Of course, in other embodiments, the second driving member 400 may be a combination of a motor and a synchronous belt mechanism; alternatively, the second driving member 400 is a linear motor; still alternatively, the second driver 400 is a cylinder. That is, a mechanism that can output a linear motion may be used as the second driver 400.

Further, a guiding structure is further provided on the lifting support 500, and the guiding structure is used for improving the lifting precision of the lifting support 500. In some embodiments, the guide structure may be a combination of a guide rail and a guide block, or the guide structure may be a combination of a guide rod and a guide hole.

With continued reference to fig. 1, the perfusion reagent apparatus further includes a waste liquid collecting member 700, the waste liquid collecting member 700 is fixedly connected to the support table 203, and the waste liquid collecting member 700 can move along the first direction along with the support table 203 under the driving of the first driving member 300, and the waste liquid tank 701 is provided on the waste liquid collecting member 700. The arrangement of the waste collection member 700 enables the priming reagent device to clean the pre-priming flow channel and to clean the post-priming flow channel. For example, before pouring, the waste liquid collecting member 700 may be moved to the lower side of the pouring needle 101 by the first driving member 300, so that the pouring needle 101 may discharge the reagent liquid flowing through the pouring channel into the waste liquid tank 701 at the beginning of the pouring, so as to avoid the pollution caused by the residual reagent liquid in the pouring channel at the last pouring operation being different from the reagent liquid injected at the current pouring operation; after the pouring is completed, the waste liquid collecting member 700 can be moved to the lower side of the liquid injection needle 101 again through the first driving member 300, so that the liquid injection needle 101 can discharge the residual reagent liquid in the liquid injection flow channel into the waste liquid groove 701 after the liquid injection, and the reagent liquid can be avoided from being remained in the liquid injection flow channel for a long time to pollute the liquid injection pipe or other liquid injection components.

Further, as shown in fig. 2 and 3, the priming reagent apparatus further includes a waste liquid discharge pipe 800, the waste liquid discharge pipe 800 is connected to one side of the waste liquid collecting member 700, and a liquid inlet end of the waste liquid discharge pipe 800 communicates with the waste liquid tank 701 so that reagent liquid in the waste liquid tank 701 can flow out of the waste liquid collecting member 700 through the waste liquid discharge pipe 800. In some embodiments, the waste pipe 800 extends downward in a diagonally downward direction, and the liquid outlet end of the waste pipe 800 is disposed downward.

With continued reference to fig. 2, the priming reagent apparatus further includes a detection mechanism 900, where the detection mechanism 900 is disposed on the priming platform 201 and is configured to detect whether the priming plate 202 is present on the priming platform 201. In some embodiments, the detection mechanism 900 is a material sensor disposed on the pouring platform 201, where the material sensor is an existing sensor, and is capable of acquiring whether the pouring plate 202 is present on the pouring platform 201, and performing a pouring operation after determining that the pouring plate 202 is present.

As shown in fig. 3, the perfusion reagent apparatus further includes a control mechanism, which may be a centralized or distributed controller 110, for example, the controller 110 may be a single-chip microcomputer or may be a distributed multi-chip microcomputer, where a control program may be run in the single-chip microcomputer, so as to control the first driving member 300, the second driving member 400, the switch valve 103, and the liquid injection pump 102 to implement functions thereof. With continued reference to fig. 3, the priming reagent device further comprises a housing 120, the housing 120 being provided on the priming platform 201, and the housing 120 being adapted to protect the electrical circuitry.

It is to be understood that the above examples of the present utility model are provided for clarity of illustration only and are not limiting of the embodiments of the present utility model. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the utility model are desired to be protected by the following claims.

Claims (9)

1. A perfusion reagent apparatus, comprising:

a liquid injection assembly (100), the liquid injection assembly (100) comprising a liquid injection needle (101) for injecting liquid;

-a perfusion assembly (200), the perfusion assembly (200) comprising a perfusion platform (201), the perfusion platform (201) being for placing a perfusion plate (202);

a first driving member (300), wherein the first driving member (300) is arranged on the pouring platform (201) and is used for driving the pouring plate (202) or the pouring needle (101) to move along a first direction;

the second driving piece (400) is arranged on the pouring platform (201) and is used for driving the liquid injection needle (101) to move along the vertical direction;

the priming reagent device further comprises a supporting table (203), the supporting table (203) is movably arranged on the priming platform (201), the supporting table (203) is used for supporting the priming plate (202), and the first driving piece (300) is in transmission connection with the supporting table (203) and is used for driving the supporting table (203) to move along the first direction.

2. The perfusion reagent apparatus of claim 1, wherein,

the liquid injection assembly (100) further comprises a liquid injection pump (102), a liquid injection pipe and a switch valve (103), one end of the liquid injection pipe is communicated with a reagent bottle containing a reagent, the other end of the liquid injection pipe is communicated with the liquid injection needle (101), and the liquid injection pump (102) and the switch valve (103) are both arranged on the liquid injection pipe.

3. The perfusion reagent apparatus of claim 1, wherein,

the liquid injection assembly (100) comprises a plurality of liquid injection needles (101) which are arranged at intervals along a second direction.

4. The perfusion reagent apparatus of claim 1, wherein,

the reagent pouring equipment further comprises a lifting support (500), the lifting support (500) comprises a vertical plate (501) and a needle support (502), the output end of the second driving piece (400) is connected with the vertical plate (501), the needle support (502) is arranged above the pouring platform (201), and the liquid pouring needle (101) is arranged on the needle support (502).

5. The perfusion reagent apparatus of claim 1, wherein,

the perfusion reagent device further comprises a guiding mechanism (600), the guiding mechanism (600) being adapted to guide the movement of the support table (203) in the first direction.

6. The perfusion reagent apparatus of claim 1, wherein,

the first driving piece (300) comprises a first driving motor (401), a driving wheel (402), a synchronous belt (403) and a driven wheel (404), wherein the first driving motor (401) is fixedly arranged on the pouring platform (201), a motor shaft of the first driving motor (401) is connected with the driving wheel (402), the driving wheel (402) and the driven wheel (404) are arranged along the first direction at intervals, the driven wheel (404) is rotationally connected onto the pouring platform (201), the synchronous belt (403) is sleeved on the driving wheel (402) and the driven wheel (404), the synchronous belt (403) is connected with the supporting table (203), and the synchronous belt (403) drives the supporting table (203) to synchronously move.

7. The perfusion reagent apparatus of claim 1, wherein,

the perfusion reagent device further comprises a waste liquid collecting member (700), the waste liquid collecting member (700) is fixedly connected with the supporting table (203), the waste liquid collecting member (700) can move along the first direction along with the supporting table (203), and a waste liquid tank (701) is arranged on the waste liquid collecting member (700).

8. The perfusion reagent apparatus of claim 7, wherein,

the perfusion reagent device further comprises a waste liquid discharge pipe (800), wherein the waste liquid discharge pipe (800) is connected to one side of the waste liquid collecting piece (700), and a liquid inlet end of the waste liquid discharge pipe (800) is communicated with the waste liquid groove (701).

9. The perfusion reagent apparatus according to any one of claims 1-8, wherein,

the pouring reagent device further comprises a detection mechanism (900), wherein the detection mechanism (900) is arranged on the pouring platform (201) and is used for detecting whether the pouring plate (202) is arranged on the pouring platform (201).