CN216192330U - Liquid separating extractor by magnetic bead method - Google Patents

Abstract

The utility model relates to a liquid separation extraction instrument by a magnetic bead method, which comprises an automatic liquid adding device, a nucleic acid extraction device and a kit conveying device, wherein the automatic liquid adding device and the nucleic acid extraction device are respectively arranged above the kit conveying device; the automatic liquid adding device comprises a liquid adding needle arranged above a reagent plate of the reagent box conveying device, the liquid adding needle is communicated with the output end of a liquid adding pump, the input end of the liquid adding pump is connected with a liquid suction pipe, and the liquid adding needle can move in the upper, lower, left and right directions relative to the reagent box conveying device; the nucleic acid extraction device comprises a magnetic rod linear motion mechanism, wherein the magnetic rod linear motion mechanism is arranged on a ball screw linear module, a magnetic sleeve is arranged below the magnetic rod linear motion mechanism on the ball screw linear module, the magnetic rod linear motion mechanism and the magnetic sleeve can move up and down relative to the ball screw linear module, a magnetic rod is fixedly arranged on the magnetic rod linear motion mechanism, and the magnetic rod can be inserted in the magnetic sleeve.

Description

Magnetic bead method liquid extractor

technical field

The utility model belongs to the technical field of magnetic bead extraction and purification, in particular to a magnetic bead method liquid separation extractor.

Background technique

The automation part of the existing magnetic bead extraction and purification technology mainly focuses on the extraction stage, that is, the extraction reagents are manually added to the deep-well plate, and after each addition, the deep-well plate with the reagents is put into the (nucleic acid) extractor for extraction. Cleavage, washing, sulfurization, elution and other steps. When the working nature of the reagents is different, (such as adding new reagents in the middle, etc.), it is also necessary to interrupt the operation during the automatic extraction process and perform manual intervention, so that personnel need to pay attention to the working process at any time, and the normal operation cannot be affected by human participation. Extraction process, otherwise, the fluency of the extraction process will be insufficient, resulting in deviation of results or even failure of extraction.

In addition, the existing (nucleic acid) extractors on the market are mainly divided into two specifications: low-throughput (nucleic acid) extractors and large-throughput (nucleic acid) extractors; low-throughput (nucleic acid) extractors are used when the number of samples per day varies In many cases, when the number of samples is small, the extraction operation can be performed at any time without accumulating enough samples to perform the operation later, which can be convenient and quick to produce results. When the number of samples per day is too large, the small-throughput (nucleic acid) extractor cannot meet the requirements of extraction and detection, so a high-throughput (nucleic acid) extractor is required, but if the number of samples is not fixed, it is often high-throughput (nucleic acid) extractor. ) extractor cannot meet the test of small throughput, which will result in waste of reagents. Therefore, no matter whether small throughput or high throughput (nucleic acid) extractor can not meet the situation that the number of samples per day is not fixed. At the same time, due to the different use of reagents, the extraction process of some reagents takes a long time. When the throughput is too small, it is often impossible to extract several samples a day. In this case, a large-throughput (nucleic acid) extractor is also required. .

For the analysis of the above situations, the magnetic bead extraction and purification technology needs to solve the following problems: First, it is necessary to reduce the occurrence of manual addition of liquids due to the requirement of reagent working conditions. Second, try to choose a high-throughput (nucleic acid) extractor to prevent the occurrence of insufficient sample extraction and low work efficiency when the sample volume is too large or the reagent extraction time is too long. Third, it is necessary to improve the large-throughput (nucleic acid) extractor on the market that must extract a large amount of samples at one time, so as to prevent the occurrence of excessive waste of reagents when the sample amount is insufficient. Fourth, it is necessary to add the on-demand and on-demand function scheme, so that the remaining unworked flux can be used for the next round of nucleic acid extraction during the nucleic acid extraction process.

Utility model content

The purpose of the utility model is to provide a magnetic bead-based liquid-separating extractor, which is designed and improved in view of the defects of the existing (nucleic acid) extractor, especially to meet the situation that the number of samples per day is not fixed.

In order to achieve the above purpose, the technical solution adopted in the present invention is: a magnetic bead method liquid separation extractor, including an automatic liquid addition device, a nucleic acid extraction device, a kit transport device, the automatic liquid addition device, and the nucleic acid extraction device. They are respectively arranged above the kit transport device;

The automatic liquid addition device includes a liquid addition needle arranged above the reagent plate of the reagent box transport device, the liquid addition needle is connected to the output end of the liquid addition pump, and the input end of the liquid addition pump is connected to the suction pipe, and the liquid addition needle is connected to the suction pipe. The liquid filling needle can be moved relative to the up, down, left and right directions of the kit transport device;

The nucleic acid extraction device includes a magnetic rod linear motion mechanism, the magnetic rod linear motion mechanism is arranged on the ball screw linear module, and the ball screw linear module is provided with a magnetic sleeve below the magnetic rod linear motion mechanism, The magnetic rod linear motion mechanism and the magnetic sleeve can move up and down relative to the ball screw linear module, and a magnetic rod is fixed on the magnetic rod linear motion mechanism, and the magnetic rod can be inserted into the magnetic sleeve, The magnetic rod linear motion mechanism drives the magnetic rod to move up and down relative to the magnetic sleeve;

The kit transporting device includes a support platform on which the automatic liquid addition device and the nucleic acid extraction device are placed, a reagent plate is arranged on the support platform, a kit holder and a kit card are arranged on the reagent plate. A reagent kit is set on the seat, and the reagent plate can move freely between the automatic liquid addition device and the nucleic acid extraction device;

The liquid addition needles and nucleic acid extraction devices are provided with multiple groups, and a corresponding reagent plate is arranged under each group of liquid addition needles and nucleic acid extraction devices.

Further, a heating device is correspondingly arranged at each group of reagent plates of the reagent box transport device, the heating device is located below the reagent box, and the heating device includes a fixed on the support platform of the reagent box transport device. The heating base is provided with a heating block, and the heating block includes a heating element. The heating screw motor drives the heating block, and the heating base can move up and down.

Still further, the liquid adding needles are multiple sets of needles arranged along the length direction of the reagent box of the reagent box transport device, and the automatic liquid adding device also includes a vertical plate arranged along the width direction of the reagent box transport device. A first synchronous belt is arranged along the length direction of the plate, and one end of the first synchronous belt is arranged with a first synchronous wheel. The first synchronous wheel is driven and rotated by a liquid-filling motor. The liquid-filling needle is arranged on the sliding block structure, so The sliding block structure is fixed on the first synchronous belt, and the first synchronous belt drives the liquid filling needle on the sliding block structure to move freely along the longitudinal direction of the vertical plate; the sliding block structure is along the vertical plate. A guide rod is set in the high direction, the liquid filling needle is set on the slider at the lower end of the guide rod, and a lifting motor is installed on the upper end of the guide rod, and the lifting motor drives the slider on which the liquid filling needle is fixed on the guide rod to move along the vertical plate. The board can move freely in the direction of height.

Still further, the lifting motor is a lead screw motor, and the liquid adding pump is a quantitative pump.

Still further, the magnetic rod linear motion mechanism and the magnetic sleeve are relatively fixedly arranged.

Still further, the ball screw linear module comprises a vertical frame placed above the reagent board of the reagent box transport device, the ball screw linear module motor is arranged above the vertical frame, and the ball screw mechanism is arranged on the vertical frame. The magnetic rod linear motion mechanism and the magnetic sleeve are arranged on the sliding seat of the ball screw mechanism, and the rotation of the ball screw linear module motor drives the magnetic rod linear motion mechanism and the magnetic sleeve to move up and down along the height direction of the stand. The magnetic rod linear motion mechanism includes a sub-sliding assembly arranged above the magnetic sleeve, the magnetic rod is arranged on the sub-slider of the sub-sliding assembly, and the linear motor above the magnetic rod linear motion mechanism drives the sub-slider The slider can move up and down relative to the magnetic sleeve.

Still further, the linear motor is a lead screw motor, the magnetic sleeve is a plurality of sets of sleeve heads arranged along the width direction of the reagent box of the reagent box transport device, and the number of the magnetic rods and the magnetic sleeves is consistent and in one-to-one correspondence. layout.

Still further, a motor is arranged under the support platform of the reagent box transport device, a second synchronous wheel is arranged on the output shaft of the motor, and a second synchronous belt is arranged on the second synchronous wheel, and the second synchronous belt is arranged along the reagent. The cartridges of the cartridge transport device are arranged in the length direction, the cartridge holder is located above the support platform, and the support platform is provided with a strip hole along the belt length direction of the second synchronous belt, and the heating device is located in the strip hole On one side of the hole, a limit support plate is fixed at the bottom of the cartridge holder, and the limit support plate passes through the strip hole and is fixedly connected with the second synchronous belt. The position support plate can move freely along the hole length direction of the strip hole.

Further, three groups of nucleic acid extraction devices are arranged at equal intervals along the width direction of the kit of the kit transport device on the support platform of the kit transport device, and three groups of reagent plates are correspondingly arranged below the three groups of nucleic acid extraction devices, Liquid addition needles are arranged above the second synchronous belts of the 3 groups of reagent plates, and 3 groups of heating devices are respectively arranged on the other ends above the second synchronous belts of the 3 groups of reagent plates.

Still further, the test kit transport device further includes a absorbent cotton swab guide seat, the water absorbent cotton swab guide seat is located at an avoidance position of the heating device, and the water absorbent cotton swab guide seat is located along the reagent box of the kit transport device. The absorbent cotton swab is arranged in the longitudinal direction of the absorbent cotton swab, and the absorbent cotton swab is arranged on the guide seat, and the liquid adding needle can be moved downward to the absorbent cotton swab guide seat.

The technical effect of the present utility model is:

1. Automatically add extraction reagents before nucleic acid extraction and during the extraction process to avoid the problem of manually adding reagents in advance or during the process.

2. Combined with the size of the instrument, a high-throughput nucleic acid extraction design is adopted.

3. Distinguish multiple reagent extraction work areas. Each reagent extraction work area is independent of each other and does not interfere with each other to solve the mutual conversion between small-throughput and large-throughput extraction. Happening.

Description of drawings

Fig. 1 is the three-dimensional structure diagram of a viewing angle of the present utility model;

Fig. 2 is the perspective structure diagram of the automatic liquid adding device of the present utility model;

3 is a three-dimensional structural view of the automatic liquid adding device of the present invention from another perspective;

4 is a three-dimensional structural diagram of the nucleic acid extraction device of the present invention from a perspective;

5 is a perspective structural view of the kit transport device of the present invention;

6 is a perspective structural view of the heating device of the present invention;

Fig. 7 is a perspective structural diagram of a set of kits to which the utility model is applied;

Figure 8 is a perspective structural diagram of another set of kits to which the utility model is applied;

FIG. 9 is a perspective structural view of the heating block of the present invention from one perspective.

Detailed ways

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

Referring to the accompanying drawings, a magnetic bead method liquid separation extractor, the device of the present invention includes an automatic liquid addition device 100, through which the automatic liquid addition of reagents in multiple regions can be performed simultaneously; the nucleic acid extraction device 200, through the nucleic acid The extraction device 200 performs nucleic acid extraction work; the kit transport device 300 cooperates with the nucleic acid extraction device 200 to perform precise movement of each hole position, which facilitates the accurate work of the nucleic acid extraction device 200 ; the heating device 400 passes through the heating device 400 In view of the need for precise temperature control of the reagents in the working process, the detection accuracy of the results of the extraction reagents is improved.

Referring specifically to FIG. 1 , the specific structure of the present invention includes four parts: an automatic liquid addition device 100 , a nucleic acid extraction device 200 , a kit transport device 300 , and a heating device 400 . The empty reagent cartridge 301 is placed on the reagent cartridge holder 302 and is directly transported under the control of the motor 303 to the automatic dosing device 100, and is inserted into the reagent box 301 by the downward movement of the dosing needle 106, and then the corresponding dosing pump 108 is used. The reagents are automatically filled into the reagent box 301, and the motor 303 of the reagent box transport device 300 can be continuously operated to change the position of the reagent box 301 under the liquid filling needle 106, so that different reagents can be added into the reagent box 301 for the next step of nucleic acid. Prepare for extraction. After the liquid in the reagent box 301 is filled, the reagent box transport device 300 moves the reagent box 301 filled with reagents to the bottom of the automatic extraction module 200, and controls the magnetic rod 205 and the magnetic sleeve 206 in the reagent according to the procedure specified in the reagent description. During the process of mixing and magnetic bead transfer in the box 301, and the nucleic acid extraction process is over, the kit 301 is automatically output and taken out for subsequent detection work.

A magnetic bead method for liquid separation and extraction apparatus, comprising an automatic liquid addition device 100, a nucleic acid extraction device 200, and a kit transport device 300. The automatic liquid addition device 100 and the nucleic acid extraction device 200 are respectively arranged above the kit transport device 300. ;

The automatic liquid addition device 100 includes a liquid addition needle 106 disposed above the reagent plate of the reagent box transport device 300 , the liquid addition needle 106 is connected to the output end of the liquid addition pump 108 , and the input end of the liquid addition pump 108 is connected. The pipette 109, the liquid adding needle 106 can be moved relative to the up, down, left and right directions of the kit transport device 300;

The nucleic acid extraction device 200 includes a magnetic rod linear motion mechanism 204, and the magnetic rod linear motion mechanism 204 is arranged on the ball screw linear module, and the ball screw linear module is located below the magnetic rod linear motion mechanism 204. There is a magnetic sleeve 206, the magnetic rod linear motion mechanism 204 and the magnetic sleeve 206 can move up and down relative to the ball screw linear module, and a magnetic rod 205 is fixed on the magnetic rod linear motion mechanism 204, and the magnetic rod 205 can be moved up and down. Inserted into the magnetic sleeve 206, the magnetic rod linear motion mechanism 204 drives the magnetic rod 205 to move up and down relative to the magnetic sleeve 206;

The kit transport device 300 includes a support platform on which the automatic liquid addition device 100 and the nucleic acid extraction device 200 are placed. A reagent plate is set on the support platform, and a kit holder 302 is set on the reagent plate. , a reagent kit 301 is set on the kit holder 302, and the reagent plate can move freely between the automatic liquid addition device 100 and the nucleic acid extraction device 200;

The liquid addition needles 106 and the nucleic acid extraction device 200 are provided with multiple groups, and each group of liquid addition needles 106 and the nucleic acid extraction device 200 is provided with a corresponding reagent plate below.

Further, a heating device 400 is correspondingly disposed at each group of reagent plates of the reagent box transport device 300 , the heating device 400 is located below the reagent box 301 , and the heating device 400 includes a device fixed to the reagent box transport device. The heating base 403 on the support platform of 300 is provided with a heating block 401. The heating block 401 includes a heating element 402. The heating screw motor 404 drives the heating block 401 and the heating base 403 to move up and down.

Still further, the liquid adding needles 106 are multiple sets of needles arranged along the length direction of the reagent box 301 of the reagent box transport device 300 , and the automatic liquid adding device 100 also includes a set of needles arranged along the width direction of the reagent box transport device 300 . On the vertical plate, a first synchronous belt 104 is arranged along the length direction of the plate, and one end of the first synchronous belt 104 is provided with a first synchronous wheel 103. The liquid needle 106 is arranged on the sliding block structure, the sliding block structure is fixed on the first synchronous belt 104, and the first synchronous belt 104 drives the liquid filling needle 106 on the sliding block structure along the length direction of the vertical plate Free movement; the sliding block structure is provided with a guide rod 107 along the height direction of the vertical plate, the liquid filling needle 106 is set on the slider at the lower end of the guide rod 107, and the upper end of the guide rod 107 is provided with a lifting motor 102, so The lift motor 102 drives the slider on the guide rod 107 on which the liquid filling needle 106 is fixed to move freely along the height direction of the vertical plate.

Still further, the lift motor 102 is a lead screw motor, and the liquid addition pump 108 is a quantitative pump.

Still further, the magnetic rod linear motion mechanism 204 and the magnetic sleeve 206 are relatively fixedly arranged.

Still further, the ball screw linear module 202 includes a stand placed above the reagent board of the reagent box transport device 300, the ball screw linear module motor 201 is arranged above the stand, and the ball screw is arranged on the stand. The magnetic rod linear motion mechanism 204 and the magnetic sleeve 206 are arranged on the sliding seat of the ball screw mechanism, and the rotation of the ball screw linear module motor 201 drives the magnetic rod linear motion mechanism 204 and the magnetic sleeve 206 along the vertical axis. The height direction of the rack can be moved up and down; the magnetic bar linear motion mechanism 204 includes a sub-sliding assembly arranged above the magnetic sleeve 206, the magnetic bar 205 is arranged on the sub-slider of the sub-sliding assembly, and the magnetic bar is linear The linear motor 203 above the motion mechanism 204 drives the sub-slider to move up and down relative to the magnetic sleeve 206 .

Still further, the linear motor 203 is a screw motor, the magnetic sleeve 206 is a plurality of sets of sleeve heads arranged along the width direction of the reagent box 301 of the reagent box transport device 300, the magnetic rod 205 and the magnetic sleeve 206 The number is the same and arranged in one-to-one correspondence.

Still further, a motor 303 is arranged under the support platform of the described reagent box transport device 300, a second synchronous wheel 304 is arranged on the output shaft of the motor 303, and a second synchronous belt 305 is arranged on the second synchronous wheel 304. The second synchronous belt 305 is arranged along the length direction of the reagent box 301 of the reagent box transport device 300 , the reagent box holder 302 is located above the support platform, and the support platform is provided with strips along the belt length direction of the second synchronous belt 305 The heating device 400 is located on one side of the strip hole, the bottom of the cartridge holder 302 is fixed with a limit support plate, and the limit support plate is fixedly connected with the second timing belt 305 The said limiting support plate can move freely along the hole length direction of the strip hole.

Further, three groups of nucleic acid extraction devices 200 are arranged at equal intervals along the width direction of the kit 301 of the kit transport device 300 on the supporting platform of the kit transport device 300 , and correspondingly provided below the three groups of nucleic acid extraction devices 200 . Three sets of reagent plates, liquid addition needles 106 are arranged above the second synchronous belts 305 of the three groups of reagent plates, and three sets of heating devices 400 are respectively disposed above the other ends of the second synchronous belts 305 of the three groups of reagent plates.

Still further, the reagent box transport device 300 further includes a absorbent cotton swab guide seat 306, and the absorbent cotton swab guide seat 306 is located at an avoidance position of the heating device 400, and the absorbent cotton swab guide seat 306 is along the reagent box. The reagent box 301 of the transport device 300 is arranged in the longitudinal direction, the absorbent cotton swab 307 is arranged on the absorbent cotton swab guide seat 306 , and the liquid adding needle 106 can be moved downward to the absorbent cotton swab guide seat 306 .

The following is a detailed description of the structure of each component:

The specific mechanism of the automatic liquid addition device 100 is as follows: the liquid addition motor 101 drives the first synchronous wheel 103 to rotate, and the rotation of the first synchronous wheel 103 can drive the first synchronous belt 104 to move laterally. Therefore, the movement of the first synchronous belt 104 is converted into the lateral movement of the whole mechanism of the liquid addition needle 106, so that the liquid addition needle 106 becomes a lateral movement. The lift motor 102 drives the liquid filling needle 106 to move vertically on the guide rod 107 as a lead screw motor. In this way, the liquid adding needle 106 can move in two directions, lateral and vertical, on this mechanism. The liquid addition pump 108 is a quantitative pump, which can accurately add liquid. Each liquid addition pump 108 is controlled to suck the reagent from the liquid suction pipe 109 and then pass the liquid addition needle 106 to accurately add liquid. As shown in 2 and 3, the automatic liquid addition device 100 is driven by the liquid addition motor 101 to drive the first synchronous wheel 103 to rotate, the first synchronous belt 104 of the first synchronization wheel 103 drives the liquid addition needle 106 to move laterally, and the lift motor 102 is a lead screw The motor can drive the liquid addition needle 106 to move vertically, which can facilitate the liquid addition needle 106 to directly enter the reagent plate of the reagent box transport device 300 for liquid addition, and prevent liquid splashes from contaminating adjacent holes.

The specific mechanism of the nucleic acid extraction device 200 is as follows: the ball screw linear module motor 201 controls the rotation of the ball screw mechanism to drive the up and down movement of the magnetic rod linear motion mechanism 204, and the magnetic sleeve 206 is fixed at the bottom of the magnetic rod linear motion mechanism 204 (( That is, the magnetic rod linear motion mechanism 204 and the magnetic sleeve 206 are fixed on the sliding seat of the ball screw mechanism). In this way, the magnetic rod linear motion mechanism 204 moves up and down and drives the magnetic sleeve 206 to also move up and down linearly. The linear motor 203 above the magnetic bar linear motion mechanism 204 is a lead screw motor. The rotation of the linear motor 203 drives the magnetic bar linear motion mechanism 204 to move up and down, and the magnetic bar 205 is fixed on the magnetic bar linear motion mechanism 204, so that the magnetic bar 205 can Relative (up and down) movement is performed relative to the magnetic sleeve 206 to facilitate insertion of the magnetic rod 205 or lifting out of the magnetic sleeve 206 . As shown in FIG. 4 , the nucleic acid extraction device 200 is mainly composed of a ball screw linear module 202 (including a ball screw linear module motor 201 ) and a magnetic rod linear motion mechanism 204 . Among them, the ball screw linear module The motor 201 drives the magnetic rod linear motion mechanism 204 to move up and down, and at the same time fixes the magnetic sleeve 206 under the magnetic rod linear motion mechanism 204. In this way, when the ball screw moves up and down, it not only drives the magnetic rod linear motion mechanism 204 to move up and down, but also simultaneously. And drive the magnetic sleeve 206 to move up and down synchronously. Among them, the magnetic bar 205 in the magnetic bar linear motion mechanism 204 can be independently controlled by the magnetic bar linear motion mechanism 204 to control the lifting movement, so that the linear motor 203 can control the magnetic bar 205 and the magnetic sleeve 206. The magnetic bar 205 and the magnetic sleeve 206 move relatively (up and down). This is the role of the magnetic sleeve 205 in the nucleic acid extraction process, that is, the deep-well plate (set on the reagent plate of the kit transport device 300, in this scheme can be the same plate as the reagent plate, of course, it can also be the same plate as needed. The high-speed movement up and down in the different plates on the reagent plate plays the role of stirring the liquid in the deep-well plate, and then mixes more efficiently. Specifically, when the magnetic rod 205 is lowered and inserted into the magnetic sleeve 206, the bottom of the magnetic sleeve 206 can generate a strong magnetic field due to the presence of the magnetic rod 205 at the bottom of the magnetic sleeve 206, so that the reagents in the reagent kit 301 can be easily adsorbed and suspended among the reagents. The magnetic particles that are completely adsorbed are lifted out of the top of the reagent box 301 by the ball screw linear module motor 201, and then cooperated with the movement of the reagent box transport device 300 to conveniently transfer the magnetic particles to the next hole of the reagent box 301. Inside, the magnetic rod linear motion mechanism 204 controls the magnetic rod 205 to lift out of the magnetic sleeve 206, so that the magnetism at the bottom of the magnetic sleeve 206 disappears, and then the ball screw linear module motor 201 controls the up and down movement of the magnetic sleeve 206 to disperse and mix. The magnetic beads are mixed in the next well 301 of the kit, so as to achieve a series of steps of magnetic beads adsorption, transport, dispersing and mixing, and complete the workflow of nucleic acid extraction.

The specific mechanism of the reagent box transport device 300 is as follows: the empty reagent box 301 is placed on the reagent box holder 302, the second synchronizing wheel 304 is driven by the rotation of the motor 303 to rotate, and then the second synchronous belt 305 is driven into a linear motion. The kit holder 302 is fixed on the belt 305, so that the motor 303 controls the kit holder 302 to perform precise linear motion, and the whole process of nucleic acid extraction can be realized with three sets of nucleic acid extraction devices 200. There are also three sets of heating devices 400 on the kit transport device 300 respectively corresponding to the heating requirements in the nucleic acid extraction process in the three regions. At the same time, there are two parts, the absorbent cotton swab 307 and the absorbent cotton swab guide seat 306 . When the automatic liquid adding device 100 is finished, the liquid adding needle 106 will have residual reagents hanging on beads, and there is a risk of contaminating other reagents, so a water-absorbing cotton swab 307 is provided to absorb the liquid beads hanging on the liquid adding needle 106, and the liquid adding needle 106 moves down to the absorbent cotton swab 307, so that the liquid droplets on the liquid adding needle 106 can be absorbed by the absorbent cotton swab 307. In addition, the absorbent cotton swab 307 can be replaced regularly. As shown in FIG. 5 , the reagent box transport device 300 is driven by three sets of second synchronous belts 305 to drive the respective reagent boxes 301 to move, corresponding to three sets of ball screw linear modules and magnetic rod linear motion mechanisms 204 , and each set of second synchronous belts 305 can be controlled by their respective motors 303, so that the three areas can be moved separately, and then the switching between small flux and large flux extraction can be realized. During the extraction process of one group, the other two groups can also be added at any time. work without interfering with each other. There is a heating device 400 in each group of reagent cartridge transport devices 300 to conveniently heat the reagents and meet the heating requirements in the reagent extraction process.

The specific mechanism of the heating device 400 is as follows: the heating block 401 is fixed with the heating base 403, the heating base 403 is controlled by the heating screw motor 404 to move up and down, and the heating screw motor 404 can move freely on the heating fixing base 405, thus realizing the heating The screw motor 404 drives the heating block 401 to move up and down. There are several groups of heating elements 402 inside the heating block 401. By controlling the start and stop of the heating elements 402, the heating and cooling power of the heating block 401 can be controlled. At the same time, there are temperature sensors and temperature protection elements inside the heating block 401. The temperature sensor is convenient for PID temperature control adjustment, and achieves stable temperature heating without unstable heating temperature. As shown in FIG. 6 , the heating device 400 is composed of a structure in which the heating screw motor 404 controls the heating block 401 to move up and down. When a certain hole in the reagent box 301 needs to be heated, the heating block 401 is lifted by the heating screw motor 404. Just fit the bottom of the reagent box 301, so that a certain hole in the reagent box 301 can be heated. When the heating is over, the heating device 400 is lowered and separated from the reagent box 301, and the heating element 402 is turned off at the same time to end the heating. The heating block 401 is lowered and separated from the reagent box 301, so that the reagent box 301 can be driven by the reagent box transport device 300 to move freely.

Further, the heating block 401 can be heated on the heating block 401, respectively, or both of the two different reagent kits 301 of the reagent kit 1 and the reagent kit 2 shown in FIG. 7 and FIG. 8 can be heated. Wherein, the first row in the kit 1 (the row closest to the heating block 401 ) can be directly placed on the heating block 401 to form a close fit and the heating block 401 conducts heat. The second kit has only a single row, which is heated in a single row, and has high heating requirements. Therefore, the heating block 401 is designed with 8 deep holes. Therefore, the design of the heating block 401 is that, firstly, both types of reagent kits 301 can be heated;

To sum up, the beneficial effects of the present utility model:

(1) The present invention uses the automatic liquid addition device 100 to fully control the liquid addition, so that the uncontrollability of the liquid addition process caused by manual liquid addition can be avoided. And it can control the liquid addition task at any node in the extraction process, so that the whole process of nucleic acid extraction is unattended and labor is freed.

(2) The utility model is composed of a plurality of (three are given in the embodiment) free extraction stations, and a plurality of (three are given in the embodiment) extraction stations respectively correspond to a plurality of (three are given in the embodiment) A nucleic acid extraction device 200, a plurality of (three in the embodiment) kit transporting devices 300 (working positions), and a plurality of (three in the embodiment) heating devices 400 are composed. In this way, it is convenient to implement nucleic acid extraction in multiple regions (three are given in the embodiment) without directly interfering with each other. In this way, when the sample size is small, one group of extraction can be performed, and when the sample size is large, three groups of extraction can be performed to meet the requirements of small throughput and large throughput at the same time. In addition, multiple groups (three groups are given in the embodiment) are independent extraction modules. At the same time, when one group is extracted, the nucleic acid extraction of the other two groups can be inserted at any time, so as to realize the requirement that the extraction work can be carried out at any time.

It will be apparent to those skilled in the art that the present invention is not limited to the details of the above-described exemplary embodiments, and that the present invention may be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention.

Claims (10)

1. The utility model provides a magnetic bead method divides liquid extraction appearance which characterized in that: the device comprises an automatic liquid adding device (100), a nucleic acid extracting device (200) and a kit conveying device (300), wherein the automatic liquid adding device (100) and the nucleic acid extracting device (200) are respectively arranged above the kit conveying device (300);

the automatic liquid adding device (100) comprises a liquid adding needle (106) arranged above a reagent plate of the reagent box conveying device (300), the liquid adding needle (106) is communicated with the output end of a liquid adding pump (108), the input end of the liquid adding pump (108) is connected with a liquid suction pipe (109), and the liquid adding needle (106) can move in the up-down direction, the left-right direction and the right direction relative to the reagent box conveying device (300);

the nucleic acid extraction device (200) comprises a magnetic rod linear motion mechanism (204), wherein the magnetic rod linear motion mechanism (204) is arranged on a ball screw linear module, a magnetic sleeve (206) is arranged below the magnetic rod linear motion mechanism (204) on the ball screw linear module, the magnetic rod linear motion mechanism (204) and the magnetic sleeve (206) can move up and down relative to the ball screw linear module, a magnetic rod (205) is fixedly arranged on the magnetic rod linear motion mechanism (204), the magnetic rod (205) can be inserted into the magnetic sleeve (206), and the magnetic rod linear motion mechanism (204) drives the magnetic rod (205) to move up and down relative to the magnetic sleeve (206);

the kit conveying device (300) comprises a supporting platform, the automatic liquid adding device (100) and the nucleic acid extracting device (200) are both arranged on the supporting platform, a reagent plate is arranged on the supporting platform, a kit clamping seat (302) is arranged on the reagent plate, a kit (301) is arranged on the kit clamping seat (302), and the reagent plate can freely move between the automatic liquid adding device (100) and the nucleic acid extracting device (200);

the liquid adding needles (106) and the nucleic acid extracting device (200) are provided with a plurality of groups, and corresponding reagent plates are arranged below each group of the liquid adding needles (106) and the nucleic acid extracting device (200).

2. The liquid separation and extraction instrument adopting the magnetic bead method according to claim 1, characterized in that: reagent box conveyer (300) every group reagent board department correspond and set up heating device (400), heating device (400) be located the below of reagent box (301), heating device (400) including set firmly heating base (403) on the supporting platform of reagent box conveyer (300), set up heating block (401) on heating base (403), heating block (401) are inside to include heating original paper (402), heating lead screw motor (404) drive heating block (401), heating base (403) can move from top to bottom.

3. The liquid separation and extraction instrument using the magnetic bead method according to claim 1 or 2, characterized in that: the automatic liquid adding device (100) further comprises a vertical plate arranged along the width direction of the kit conveying device (300), a first synchronous belt (104) is arranged on the vertical plate along the length direction of the plate, one end of the first synchronous belt (104) is provided with a first synchronous wheel (103), the first synchronous wheel (103) is driven by a liquid adding motor (101) to rotate, the liquid adding needle (106) is arranged on a sliding block structure, the sliding block structure is fixedly arranged on the first synchronous belt (104), and the first synchronous belt (104) drives the liquid adding needle (106) on the sliding block structure to freely move along the length direction of the vertical plate; the structure of the sliding block is provided with a guide rod (107) along the height direction of the vertical plate, the liquid adding needle (106) is arranged on a sliding block at the lower end of the guide rod (107), the upper end of the guide rod (107) is provided with a lifting motor (102), and the lifting motor (102) drives the sliding block on the guide rod (107) and fixedly provided with the liquid adding needle (106) to freely move along the height direction of the vertical plate.

4. The liquid separation and extraction instrument adopting the magnetic bead method according to claim 3, characterized in that: the lifting motor (102) is a lead screw motor, and the liquid adding pump (108) is a fixed displacement pump.

5. The liquid separation and extraction instrument using the magnetic bead method according to claim 1 or 2, characterized in that: the magnetic rod linear motion mechanism (204) and the magnetic sleeve (206) are relatively fixedly arranged.

6. The liquid separation and extraction instrument adopting the magnetic bead method according to claim 5, characterized in that: the ball screw linear module comprises a vertical frame arranged above a reagent plate of the reagent box conveying device (300), a ball screw linear module motor (201) is arranged above the vertical frame, a ball screw mechanism is arranged on the vertical frame, the magnetic rod linear motion mechanism (204) and the magnetic sleeve (206) are arranged on a sliding seat of the ball screw mechanism, and the rotation of the ball screw linear module motor (201) drives the magnetic rod linear motion mechanism (204) and the magnetic sleeve (206) to move up and down along the height direction of the vertical frame; the magnetic rod linear motion mechanism (204) comprises a sub-sliding assembly arranged above the magnetic sleeve (206), the magnetic rod (205) is arranged on a sub-sliding block of the sub-sliding assembly, and a linear motor (203) above the magnetic rod linear motion mechanism (204) drives the sub-sliding block to move up and down relative to the magnetic sleeve (206).

7. The liquid separation and extraction instrument adopting the magnetic bead method according to claim 6, characterized in that: the linear motor (203) is a screw motor, the magnetic sleeves (206) are multiple groups of sleeve heads arranged along the width direction of the reagent kit (301) of the reagent kit conveying device (300), and the magnetic rods (205) and the magnetic sleeves (206) are consistent in quantity and are arranged in a one-to-one correspondence manner.

8. The liquid separation and extraction instrument adopting the magnetic bead method according to claim 2, characterized in that: the kit conveying device is characterized in that a motor (303) is arranged below a supporting platform of the kit conveying device (300), a second synchronous wheel (304) is arranged on an output shaft of the motor (303), a second synchronous belt (305) is arranged on the second synchronous wheel (304), the second synchronous belt (305) is arranged along the length direction of a kit (301) of the kit conveying device (300), the kit clamping seat (302) is positioned on the supporting platform, a strip-shaped hole is formed in the supporting platform along the strip length direction of the second synchronous belt (305), a heating device (400) is positioned on one side of the hole portion of the strip-shaped hole, a limiting supporting plate is fixedly arranged at the bottom of the kit clamping seat (302), the limiting supporting plate penetrates through the strip-shaped hole to be fixedly connected with the second synchronous belt (305), and the limiting supporting plate can freely move along the hole length direction of the strip-shaped hole.

9. The liquid separation and extraction instrument using the magnetic bead method according to claim 1 or 2, characterized in that: the kit conveying device is characterized in that 3 groups of nucleic acid extracting devices (200) are arranged on a supporting platform of the kit conveying device (300) at equal intervals along the width direction of a kit (301) of the kit conveying device (300), 3 groups of reagent plates are correspondingly arranged below the 3 groups of nucleic acid extracting devices (200), a liquid adding needle (106) is arranged above a second synchronous belt (305) of the 3 groups of reagent plates, and 3 groups of heating devices (400) are respectively arranged at the other ends of the tops of the second synchronous belts (305) of the 3 groups of reagent plates.

10. The liquid separation and extraction instrument adopting the magnetic bead method according to claim 9, characterized in that: the kit conveyer (300) still include absorbent cotton swab guide holder (306), absorbent cotton swab guide holder (306) be located the position of dodging of heating device (400), absorbent cotton swab guide holder (306) arrange along the length direction of kit (301) of kit conveyer (300), set up absorbent cotton swab (307) on absorbent cotton swab guide holder (306), liquid adding needle (106) can move to absorbent cotton swab guide holder (306) department downwards.

Images