CN213295303U - Full-automatic sealed fluorescent quantitative PCR instrument - Google Patents
Full-automatic sealed fluorescent quantitative PCR instrument Download PDFInfo
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- CN213295303U CN213295303U CN202021118072.3U CN202021118072U CN213295303U CN 213295303 U CN213295303 U CN 213295303U CN 202021118072 U CN202021118072 U CN 202021118072U CN 213295303 U CN213295303 U CN 213295303U
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Abstract
The utility model provides a full-automatic sealing fluorescence quantitative PCR instrument, which comprises a PCR instrument host, wherein the PCR instrument host comprises a body, an adsorption mechanism is arranged on the inner wall of the top of the body, a workbench is arranged on the side wall of the body, and a film mechanism, a cutting mechanism, a test tube rack and a control box are arranged in the same direction at the bottom of the body near one side of the workbench; the worktable is provided with a cutting hole and a film hole, the adsorption mechanism comprises a base fixedly connected with the top of the body, a rolling screw, a lifting arm and a vacuum chuck, one end of the lifting arm is connected with the base through the rolling screw, and the vacuum chuck is arranged at the other end of the lifting arm; the film mechanism comprises a bracket fixedly connected with the bottom of the body, and a film wheel core is arranged at the upper end of the bracket through a film hole; the cutting mechanism comprises an air cylinder, a lower cutter and an upper cutter which are arranged at the bottom of the body, the lower cutter is arranged at one end, away from the bottom of the body, of the air cylinder, and the upper cutter is arranged at the top of the body. The utility model discloses simple structure, the energy saving, available film is automatic seals for the test-tube rack.
Description
Technical Field
The utility model belongs to the technical field of medical equipment, especially, relate to a full-automatic fluorescence quantitative PCR appearance that seals.
Background
The real-time fluorescent quantitative PCR instrument consists of a fluorescent quantitative system and a computer and is used for monitoring the fluorescence in the circulating process. A computer connected to the real-time device collects the fluorescence data, which is displayed graphically by developed real-time analysis software. Raw data is plotted as fluorescence intensity versus cycle number, analysis can begin after raw data is collected, and real-time device software can normalize the collected data to compensate for background fluorescence differences. Most of fluorescence quantitative PCR appearance now all need the experimenter to seal the test-tube rack is manual, later put into fluorescence quantitative PCR appearance with the test-tube rack again, and whole experimentation operation is inconvenient like this, and the error is many and causes great error influence to experimental data easily.
Disclosure of Invention
In view of this, the present invention provides a full-automatic sealing fluorescence quantitative PCR instrument, so as to provide a fluorescence quantitative PCR instrument capable of automatically sealing a test tube rack in a fluorescence quantitative system.
In order to achieve the above purpose, the technical scheme of the utility model is realized like this:
a full-automatic sealed fluorescent quantitative PCR instrument comprises a PCR instrument host, an adsorption mechanism, a workbench, a film mechanism, a cutting mechanism, a test tube rack and a control box, wherein the PCR instrument host comprises a body with a cover, the adsorption mechanism is installed on the inner wall of the top of the body, and the film mechanism, the cutting mechanism, the test tube rack and the control box are sequentially arranged at the bottom of the body; an adsorption mechanism is arranged on the inner wall of the top of the body, a workbench is arranged on the inner side wall of the body, a film mechanism, a cutting mechanism, a test tube rack and a control box are sequentially arranged at the bottom of the body, and the film mechanism is arranged on one side close to the workbench; the worktable is provided with a cutting hole and a film hole; the adsorption mechanism comprises a base fixedly connected with the top of the body, a rolling screw horizontally arranged below the base, a lifting arm vertically connected with one end of the rolling screw and a vacuum chuck arranged at the lower end of the lifting arm; the film mechanism comprises a support fixedly connected with the bottom of the body, and a film wheel core is mounted at the upper end of the support through the film hole; the cutting mechanism comprises an air cylinder, a lower cutter and an upper cutter which are arranged at the bottom of the body, the lower cutter is arranged at the top end of the air cylinder, the lower cutter is arranged between the lifting arm and the support, and the upper cutter and the lower cutter are arranged at the top of the body along the same axis.
Furthermore, a sucker in the vacuum sucker is communicated with a vacuum device through a connecting pipe, and the sucker is made of nitrile rubber.
Further, a controller and a circuit board are arranged in the control box, and the air cylinder and the rolling screw are connected to the controller through signals.
Furthermore, a film is wound on the film wheel core, and the film is a transparent plastic film.
Furthermore, the upper cutter is fixedly connected with the top of the body through an upper connecting rod.
Further, the cylinder includes cylinder and the piston rod of being connected with the cylinder piston, the cylinder pass through the cylinder base with body bottom fixed connection, the bottom tool is installed to the piston rod top.
Further, the test-tube rack bottom surface is equipped with the hot plate, the hot plate lower extreme pass through the test tube base with body bottom fixed connection.
Compared with the prior art, the utility model has the advantages of following:
(1) full-automatic fluorescence quantitative PCR appearance that seals, simple structure, the energy saving can seal the test-tube rack is automatic in fluorescence quantitative system, when avoiding using traditional fluorescence quantitative PCR appearance, manual operation causes great error to experimental data.
(2) Vacuum chuck, start vacuum device suction and make vacuum chuck internal gas pressure for the burden, be about to the film and adsorb firmly, wait to adsorb mechanism and transport the film back on the test-tube rack, utilize vacuum chuck in vacuum device inflate into vacuum chuck steadily, make in the vacuum chuck become zero atmospheric pressure or slightly positive atmospheric pressure by negative atmospheric pressure compress tightly the film at the test-tube rack mouth.
Drawings
The accompanying drawings, which form a part hereof, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention without undue limitation. In the drawings:
FIG. 1 is a schematic structural view of a full-automatic sealed fluorescence quantitative PCR instrument according to an embodiment of the present invention;
fig. 2 is a schematic structural view of a workbench according to an embodiment of the present invention.
Description of reference numerals:
1. a PCR instrument host; 2. an adsorption mechanism; 21. a base; 22. a rolling screw; 23. a lifting arm; 24. a vacuum chuck; 3. a work table; 31. cutting the hole; 32. a film hole; 4. a film mechanism; 41. a support; 42. a thin film wheel core; 5. a cutting mechanism; 51. a cylinder; 52. a lower cutter; 53. feeding a cutter; 6. a test tube rack; 7. a control box; 8. heating plates; 9. a test tube base.
Detailed Description
It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
A full-automatic sealing fluorescence quantitative PCR instrument is shown in figures 1-2 and comprises a PCR instrument host 1, an adsorption mechanism 2, a workbench 3, a film mechanism 4, a cutting mechanism 5, a test tube rack 6 and a control box 7, wherein the PCR instrument host 1 comprises a body with a cover, the adsorption mechanism 2 is installed on the inner wall of the top of the body, the workbench 3 is installed on the side wall of the body, and the film mechanism 4, the cutting mechanism 5, the test tube rack 6 and the control box 7 are sequentially arranged at the bottom of the body close to one side of the workbench 3 in the same direction; the worktable 3 is provided with a cutting hole 31 and a film hole 32, the adsorption mechanism 2 comprises a base 21 fixedly connected with the top of the body, a rolling screw 22, a lifting arm 23 and a vacuum chuck 24, one end of the lifting arm 23 is connected with the base 21 through the rolling screw 22, and the other end of the lifting arm 23 is provided with the vacuum chuck 24; the film mechanism 4 comprises a bracket 41 fixedly connected with the bottom of the body, and a film wheel core 42 is arranged at the upper end of the bracket 41 through the film hole 32; the cutting mechanism 5 comprises an air cylinder 51, a lower cutter 52 and an upper cutter 53 which are arranged at the bottom of the body, wherein the lower cutter 52 is arranged at one end, far away from the bottom of the body, of the air cylinder 51, and the upper cutter 53 is arranged at the top of the body. Simple structure, energy saving can seal the test-tube rack 6 automatically in the fluorescence quantitative system, and when avoiding using the traditional fluorescence quantitative PCR instrument, the manual operation causes great error to the experimental data
The control box 7 is provided with a controller, an electric element such as a circuit board, and the like, and the air cylinder 51 and the rolling screw 22 are in signal connection with the controller.
The film wheel core 42 is provided with a film which is a transparent plastic film.
The upper cutter 53 is fixedly connected with the top of the body through an upper connecting rod.
The cylinder 51 comprises a cylinder barrel and a piston rod connected with a piston of the cylinder barrel, the cylinder barrel is fixedly connected with the bottom of the body through a base of the cylinder 51, and a lower cutter 52 is installed at one end, far away from the cylinder barrel, of the piston rod.
The test-tube rack 6 through hot plate 8, test tube base 9 with body bottom fixed connection, hot plate 8 provides temperature compensation for special reaction in the test-tube rack 6.
A full-automatic sealing fluorescence quantitative PCR instrument is disclosed, as shown in figures 1 to 2, an adsorption mechanism 2 works, a lifting arm 23 puts down a vacuum sucker 24 to a workbench 3 surface to contact with a film, the vacuum sucker 24 starts an adsorption mode under the control of a controller, at the moment, the sucker in the vacuum sucker 24 is connected with a vacuum device through a connecting pipe and then contacts with the film, the vacuum device is started to suck, negative air pressure is generated in the sucker, the film is firmly sucked, the lifting arm 23 is driven by a rolling screw rod 22 to translate to a position where the film fully covers a test tube rack 6 opening, then an air cylinder 51 receives a controller signal to start working, the air cylinder 51 drives a lower cutter 52 to move upwards to penetrate through a cutting hole 31 to push the film to contact with an upper cutter 53 until the upper cutter 52 finishes cutting off the film, and the controller controls the air cylinder 51 to return to move to the; finally, the controller controls the vacuum setting in the vacuum chuck 24 to be stably inflated into the vacuum chuck 24, so that the negative air pressure in the vacuum chuck 24 is changed into zero air pressure or slightly positive air pressure, the vacuum chuck 24 is separated from the film, and the vacuum chuck 24 is separated from the film under the action of the lifting arm 23.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. Full-automatic fluorescence quantitative PCR appearance that seals, its characterized in that: the PCR detection device comprises a PCR instrument host (1), an adsorption mechanism (2), a workbench (3), a film mechanism (4), a cutting mechanism (5), a test tube rack (6) and a control box (7), wherein the PCR instrument host (1) comprises a body with a cover, the adsorption mechanism (2) is installed on the inner wall of the top of the body, and the film mechanism (4), the cutting mechanism (5), the test tube rack (6) and the control box (7) are sequentially arranged at the bottom of the body;
an adsorption mechanism (2) is arranged on the inner wall of the top of the body, a workbench (3) is arranged on the inner side wall of the body, a film mechanism (4), a cutting mechanism (5), a test tube rack (6) and a control box (7) are sequentially arranged at the bottom of the body, and the film mechanism (4) is arranged on one side close to the workbench (3); the worktable (3) is provided with a cutting hole (31) and a film hole (32);
the adsorption mechanism (2) comprises a base (21) fixedly connected with the top of the body, a rolling screw rod (22) horizontally arranged below the base (21), a lifting arm (23) vertically connected with one end of the rolling screw rod (22) and a vacuum sucker (24) arranged at the lower end of the lifting arm (23);
the film mechanism (4) comprises a support (41) fixedly connected with the bottom of the body, and a film wheel core (42) is installed at the upper end of the support (41) through the film hole (32);
the cutting mechanism (5) comprises an air cylinder (51), a lower cutter (52) and an upper cutter (53) which are arranged at the bottom of the body, the lower cutter (52) is arranged at the top end of the air cylinder (51), the lower cutter (52) is arranged between the lifting arm (23) and the support (41), and the upper cutter (53) and the lower cutter (52) are arranged at the top of the body along the same axis.
2. The full-automatic sealed fluorescent quantitative PCR instrument according to claim 1, characterized in that: and a sucker in the vacuum sucker (24) is communicated with a vacuum device through a connecting pipe, and the sucker is made of nitrile rubber.
3. The full-automatic sealed fluorescent quantitative PCR instrument according to claim 1, characterized in that: a controller and a circuit board are arranged in the control box (7), and the air cylinder (51) and the rolling screw rod (22) are in signal connection with the controller.
4. The full-automatic sealed fluorescent quantitative PCR instrument according to claim 1, characterized in that: the film wheel core (42) is wound with a film, and the film is a transparent plastic film.
5. The full-automatic sealed fluorescent quantitative PCR instrument according to claim 1, characterized in that: the upper cutter (53) is fixedly connected with the top of the body through an upper connecting rod.
6. The full-automatic sealed fluorescent quantitative PCR instrument according to claim 1, characterized in that: the cylinder (51) comprises a cylinder barrel and a piston rod connected with a piston of the cylinder barrel, the cylinder barrel is fixedly connected with the bottom of the body through a base of the cylinder (51), and a lower cutter (52) is installed at the top end of the piston rod.
7. The full-automatic sealed fluorescent quantitative PCR instrument according to claim 1, characterized in that: the bottom surface of the test tube rack (6) is provided with a heating plate (8), and the lower end of the heating plate is fixedly connected with the bottom of the body through a test tube base (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021118072.3U CN213295303U (en) | 2020-06-16 | 2020-06-16 | Full-automatic sealed fluorescent quantitative PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021118072.3U CN213295303U (en) | 2020-06-16 | 2020-06-16 | Full-automatic sealed fluorescent quantitative PCR instrument |
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| Publication Number | Publication Date |
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| CN213295303U true CN213295303U (en) | 2021-05-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202021118072.3U Active CN213295303U (en) | 2020-06-16 | 2020-06-16 | Full-automatic sealed fluorescent quantitative PCR instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117126727A (en) * | 2023-09-18 | 2023-11-28 | 合肥达徽基因科技有限公司 | Full-automatic extraction amplification hybridization integrated workstation |
-
2020
- 2020-06-16 CN CN202021118072.3U patent/CN213295303U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117126727A (en) * | 2023-09-18 | 2023-11-28 | 合肥达徽基因科技有限公司 | Full-automatic extraction amplification hybridization integrated workstation |
| CN117126727B (en) * | 2023-09-18 | 2024-04-19 | 合肥达徽基因科技有限公司 | Full-automatic extraction amplification hybridization integrated workstation |
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