CN214361340U - Device for purifying 384 primers through positive pressure blow filling - Google Patents
Device for purifying 384 primers through positive pressure blow filling Download PDFInfo
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- CN214361340U CN214361340U CN202120442284.5U CN202120442284U CN214361340U CN 214361340 U CN214361340 U CN 214361340U CN 202120442284 U CN202120442284 U CN 202120442284U CN 214361340 U CN214361340 U CN 214361340U
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Abstract
The utility model discloses a device for filling and purifying 384 primers through positive pressure, which comprises a liquid storage bottle, a first air pipe, a material conveying pipe and a liquid adding head, wherein one end of the first air pipe penetrates through the top wall of the liquid storage bottle, the first air pipe is communicated with the inside of the liquid storage bottle, one end of the material conveying pipe penetrates through the top wall of the liquid storage bottle and extends to the bottom end of the inside of the liquid storage bottle, the other end of the material conveying pipe is communicated with the liquid adding head, one side of the liquid adding head is provided with a positive pressure gas blowing device, the top of the positive pressure gas blowing device is provided with a second air pipe, and the bottom of the positive pressure gas blowing device is provided with a guide rail; the utility model discloses, through setting up solenoid valve control liquid feeding volume, the liquid feeding volume is accurate, and the liquid feeding is efficient, through setting up malleation gas blowing device, adopts high-pressure nitrogen gas to blow-fill 384 downthehole liquid for wash, elution time shorten, promote whole production efficiency, this device simple structure, the operation is convenient, and the human input is few, low in production cost, and product quality is high simultaneously, and the product uniformity is good.
Description
Technical Field
The utility model belongs to the technical field of bioengineering equipment, specifically, relate to a device through malleation hydraulic reclamation purification 384 primers.
Background
DNA is one of the nucleic acids of one of four biological macromolecules contained in biological cells, carries the genetic information necessary for the synthesis of RNA and proteins, and is a biological macromolecule essential for the development and normal operation of organisms. Gene synthesis refers to a technique for artificially synthesizing a double-stranded DNA molecule in vitro, which is different from oligonucleotide synthesis: oligonucleotides are single-stranded, the longest fragment that can be synthesized is only about 100nt, whereas gene synthesis is double-stranded DNA molecule synthesis, which can be synthesized in the length range of 50bp-12 kb. Gene synthesis is a technique for artificially synthesizing a gene, is one of means for gene acquisition, and is not limited by a gene source because it does not require a template for gene synthesis as compared with the gene acquisition from an existing organism.
At present, a DNA synthesis column usually adopts a single-column tube or a 96-hole filter plate, the existing single-column tube has multiple specifications and large volume, is not beneficial to batch, large-scale and miniaturized operation, and is difficult to realize for trace, ultra-small and large-batch DNA synthesis; the current 96-hole filter plate mainly has two kinds of specifications of 1.5 and 2ml, can accomplish the synthetic work of 96 DNA samples simultaneously, it is to the synthesis of batched DNA, it is swift a lot more than the single tube, but on the sample volume of handling, still can't satisfy the trace, the batched of ultramicro DNA, large-scale synthesis, 384 synthetic board has been researched and developed for this reason, however current 384 synthetic version blow-fill purification operation, mostly through manual or electronic multichannel pipettor liquid feeding and negative pressure suction filtration operation or the automatic pipettor liquid feeding of 384, manual work is taken on the suction filtration device and is carried out the suction filtration again, current mode of production, the liquid feeding precision is not enough, and production efficiency is low. Therefore, it is highly desirable to develop a 384-plate blow-fill purification device with high precision and high efficiency.
SUMMERY OF THE UTILITY MODEL
For the 384 composite board hydraulic reclamation purification process liquid feeding precision that exists among the solution prior art not enough and the low technical problem of production efficiency, the utility model provides a through the device of malleation hydraulic reclamation purification 384 primers.
The purpose of the utility model can be realized by the following technical scheme:
a device for purifying 384 primers through positive pressure blow filling comprises a liquid storage bottle, a first air pipe, a conveying pipe and a liquid adding head, wherein one end of the first air pipe penetrates through the top wall of the liquid storage bottle, the first air pipe is communicated with the interior of the liquid storage bottle, one end of the conveying pipe penetrates through the top wall of the liquid storage bottle and extends to the bottom end of the interior of the liquid storage bottle, the other end of the conveying pipe is communicated with the liquid adding head, a positive pressure blowing device is arranged on one side of the liquid adding head, a second air pipe is arranged at the top of the positive pressure blowing device, and a guide rail is arranged at the bottom of the positive pressure blowing device;
the positive pressure air blowing device comprises a waste liquid tank and a tank cover, wherein an installation groove is formed in the inner side wall of the upper end of the waste liquid tank, a liquid discharge pipe is installed on the side wall of the lower end of the waste liquid tank and communicated with the interior of the waste liquid tank, a sliding block is fixedly installed on the outer wall of the bottom of the waste liquid tank, the waste liquid tank is slidably installed with a guide rail through the sliding block, the tank cover is arranged right above the waste liquid tank, the bottom end of a second air pipe penetrates through the center of the tank cover and is communicated with the interior of the waste liquid tank, an air cylinder is arranged below two sides of the tank cover, which are perpendicular to the guide rail, and the tank cover is fixedly connected with the output end of the air cylinder;
the liquid feeding head is of a closed rectangular box-shaped structure, a plurality of liquid feeding pipes are installed at the bottom of the liquid feeding head, and the liquid feeding pipes are communicated with the inside of the liquid feeding head.
As a further aspect of the present invention: and the delivery pipe is provided with an electromagnetic valve.
As a further aspect of the present invention: the edge of the bottom wall of the tank cover is embedded with a sealing ring, and the sealing ring is arranged opposite to the tank wall at the top of the waste liquid tank.
As a further aspect of the present invention: and the first air pipe and the second air pipe are both provided with pressure regulating valves.
The utility model has the advantages that:
1. the utility model provides a pair of device through malleation hydraulic reclamation purification 384 primers is through setting up solenoid valve control liquid feeding volume, is connected solenoid valve and common control module to cooperation system software realizes accurate liquid feeding, than the manual or automatic pipettor operation of tradition, and the liquid feeding volume is accurate, and liquid feeding efficiency obviously promotes.
2. The utility model provides a pair of device through malleation blow packing purification 384 primer through setting up malleation gas blowing device, adopts high-pressure nitrogen gas to carry out the blow packing to the downthehole liquid of 384 synthetic boards that advances of dropwise add for wash, elution time shorten, promote whole production efficiency.
3. The utility model provides a pair of device through malleation hydraulic reclamation purification 384 primers, the device is simple, and the operation is convenient, reduces the human input, reduction in production cost promotes product quality simultaneously, guarantees the product uniformity.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic diagram of the structure of a device for purifying 384 primers by positive pressure blow-filling according to the present invention;
FIG. 2 is a schematic structural view of a positive pressure blowing device;
FIG. 3 is a schematic view of the liquid feeding head.
In the drawings, the components represented by the respective reference numerals are listed below:
10. a liquid storage bottle; 20. a first air pipe; 30. a delivery pipe; 40. an electromagnetic valve; 50. a liquid adding head; 51. a liquid feeding pipe; 60. a second air pipe; 70. a positive pressure blowing device; 71. a waste liquid tank; 72. mounting grooves; 73. a slot cover; 74. a seal ring; 75. a slider; 76. a liquid discharge pipe; 80. a guide rail; 90. and a cylinder.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.
Referring to fig. 1-3, a device for purifying 384 primers by positive pressure blow filling comprises a liquid storage bottle 10, a first air tube 20, a material conveying tube 30 and a liquid adding head 50, wherein one end of the first air tube 20 penetrates through the top wall of the liquid storage bottle 10, the first air tube 20 is communicated with the interior of the liquid storage bottle 10, one end of the material conveying tube 30 penetrates through the top wall of the liquid storage bottle 10 and extends to the bottom end of the interior of the liquid storage bottle 10, the other end of the material conveying tube 30 is communicated with the liquid adding head 50, a positive pressure blowing device 70 is arranged on one side of the liquid adding head 50, a second air tube 60 is arranged on the top of the positive pressure blowing device 70, and a guide rail 80 is arranged at the bottom of the positive pressure blowing device 70;
the positive pressure air blowing device 70 comprises a waste liquid tank 71 and a tank cover 73, wherein the inner side wall of the upper end of the waste liquid tank 71 is provided with an installation groove 72, the side wall of the lower end of the waste liquid tank 71 is provided with a liquid discharge pipe 76, the liquid discharge pipe 76 is communicated with the interior of the waste liquid tank 71, the outer wall of the bottom of the waste liquid tank 71 is fixedly provided with a slide block 75, the waste liquid tank 71 is slidably installed with a guide rail 80 through the slide block 75, the tank cover 73 is arranged right above the waste liquid tank 71, the bottom end of the second air pipe 60 penetrates through the center of the tank cover 73 to be communicated with the interior of the waste liquid tank 71, air cylinders 90 are arranged below two sides of the tank cover 73 perpendicular to the guide rail 80, and the tank cover 73 is fixedly connected with the output ends of the air cylinders 90;
the liquid adding head 50 is of a closed rectangular box-shaped structure, a plurality of liquid adding pipes 51 are mounted at the bottom of the liquid adding head 50, and the liquid adding pipes 51 are communicated with the inside of the liquid adding head 50.
The delivery pipe 30 is provided with an electromagnetic valve 40; the electromagnetic valve 40 is connected with a common control module and is matched with system software to realize accurate liquid adding, compared with the traditional manual or automatic liquid shifter operation, the liquid adding amount is accurate, the liquid adding efficiency is obviously improved, and the control module and the system software are common existing technical equipment and are not described herein.
The edge of the bottom wall of the tank cover 73 is embedded with a sealing ring 74, and the sealing ring 74 is arranged opposite to the tank wall at the top of the waste liquid tank 71; the sealing performance is ensured, no air leakage occurs in the hydraulic filling process, and the hydraulic filling quality and efficiency are improved.
The first air pipe 20 and the second air pipe 60 are both provided with pressure regulating valves; facilitating pressure regulation for the pipeline.
The working principle is as follows:
the utility model discloses during the use, clamp the 384 synthetic orifice plates after the ammonolysis to mounting groove 72 in, adjust the inlet pressure of first trachea 20 and second trachea 60, set up the parameter of liquid feeding through control module, first trachea 20 lets in high-pressure nitrogen gas, nitrogen gas forms the high-pressure region above liquid storage bottle 10, with the liquid of liquid storage bottle 10 bottom from conveying pipeline 30 forced lubrication to solenoid valve 40 department, solenoid valve 40 is controlled by control module, let in liquid with liquid head 50 inside according to the set quantity, again through liquid feeding pipe 51 dropwise add to the corresponding hole site of orifice plate, accomplish the liquid feeding process; the orifice plate after liquid adding is conveyed to the position right below the groove cover 73 through the guide rail 80, the output end of the air cylinder 90 moves downwards to drive the groove cover 73 to move downwards, the sealing ring 74 is tightly pressed and attached to the top of the waste liquid groove 71, the air cylinder 90 stops, set high-pressure nitrogen is introduced into the second air pipe 60 to blow liquid in the orifice plate hole into the waste liquid groove 71 below, and the waste liquid is discharged through the liquid discharge pipe 76, so that the cleaning process can be completed; after the cleaning is finished, the air cylinder 90 is reset upwards, and the orifice plate is moved out to one side of the liquid adding head 50 far away from the tank cover 73 through the guide rail 80, so that the resetting is finished.
The utility model has reasonable design, controls the liquid adding volume by arranging the electromagnetic valve 40, connects the electromagnetic valve 40 with a common control module, and is matched with system software to realize accurate liquid adding, compared with the traditional manual or automatic pipettor operation, the liquid adding quantity is accurate, and the liquid adding efficiency is obviously improved; by arranging the positive pressure blowing device 70 and adopting high-pressure nitrogen to blow and fill the liquid dripped into the 384 composite plate holes, the cleaning and elution time is shortened, and the overall production efficiency is improved; this device simple structure, the operation is convenient, reduces the human input, reduction in production cost promotes product quality simultaneously, guarantees the product uniformity.
The foregoing is merely exemplary and illustrative of the structure of the invention and various modifications, additions or substitutions as are known to those skilled in the art may be made to the described embodiments without departing from the scope of the invention as defined in the accompanying claims.
Claims (4)
1. A device for purifying 384 primers by positive pressure blow filling is characterized in that: the liquid filling device comprises a liquid storage bottle (10), a first air pipe (20), a conveying pipe (30) and a liquid filling head (50), wherein one end of the first air pipe (20) penetrates through the top wall of the liquid storage bottle (10), the first air pipe (20) is communicated with the inside of the liquid storage bottle (10), one end of the conveying pipe (30) penetrates through the top wall of the liquid storage bottle (10) and extends to the bottom end of the inside of the liquid storage bottle (10), the other end of the conveying pipe (30) is communicated with the liquid filling head (50), a positive pressure air blowing device (70) is arranged on one side of the liquid filling head (50), a second air pipe (60) is arranged at the top of the positive pressure air blowing device (70), and a guide rail (80) is arranged at the bottom of the positive pressure air blowing device (70);
the positive pressure air blowing device (70) comprises a waste liquid tank (71) and a tank cover (73), wherein an installation groove (72) is formed in the inner side wall of the upper end of the waste liquid tank (71), a liquid discharge pipe (76) is installed on the side wall of the lower end of the waste liquid tank (71), the liquid discharge pipe (76) is communicated with the interior of the waste liquid tank (71), a sliding block (75) is fixedly installed on the outer wall of the bottom of the waste liquid tank (71), the waste liquid tank (71) is slidably installed with a guide rail (80) through the sliding block (75), the tank cover (73) is arranged right above the waste liquid tank (71), the bottom end of a second air pipe (60) penetrates through the center of the tank cover (73) and is communicated with the interior of the waste liquid tank (71), an air cylinder (90) is arranged below two sides, which are perpendicular to the guide rail (80), of the tank cover (73) and is fixedly connected with the output end of the air cylinder (90);
the liquid adding head (50) is of a closed rectangular box-shaped structure, a plurality of liquid adding pipes (51) are mounted at the bottom of the liquid adding head (50), and the liquid adding pipes (51) are communicated with the inside of the liquid adding head (50).
2. The apparatus for purifying 384 primers as defined in claim 1, wherein: and an electromagnetic valve (40) is arranged on the material conveying pipe (30).
3. The apparatus for purifying 384 primers as defined in claim 1, wherein: and a sealing ring (74) is embedded in the edge of the bottom wall of the tank cover (73), and the sealing ring (74) is arranged opposite to the tank wall at the top of the waste liquid tank (71).
4. The apparatus for purifying 384 primers as defined in claim 1, wherein: the first air pipe (20) and the second air pipe (60) are both provided with pressure regulating valves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120442284.5U CN214361340U (en) | 2021-03-01 | 2021-03-01 | Device for purifying 384 primers through positive pressure blow filling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120442284.5U CN214361340U (en) | 2021-03-01 | 2021-03-01 | Device for purifying 384 primers through positive pressure blow filling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214361340U true CN214361340U (en) | 2021-10-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120442284.5U Active CN214361340U (en) | 2021-03-01 | 2021-03-01 | Device for purifying 384 primers through positive pressure blow filling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214361340U (en) |
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2021
- 2021-03-01 CN CN202120442284.5U patent/CN214361340U/en active Active
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Address after: 239000 No. 69, qifushi West Road, Chuzhou economic and Technological Development Zone, Anhui Province Patentee after: General Biology (Anhui) Co., Ltd Address before: 239000 No. 69, qifushi West Road, Chuzhou economic and Technological Development Zone, Anhui Province Patentee before: General biological system (Anhui) Co., Ltd |
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| CP01 | Change in the name or title of a patent holder |