CN115452507B - Array biochip sample application device - Google Patents
Array biochip sample application device Download PDFInfo
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- CN115452507B CN115452507B CN202211066755.2A CN202211066755A CN115452507B CN 115452507 B CN115452507 B CN 115452507B CN 202211066755 A CN202211066755 A CN 202211066755A CN 115452507 B CN115452507 B CN 115452507B
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- 238000000018 DNA microarray Methods 0.000 title claims abstract description 19
- 239000007921 spray Substances 0.000 claims abstract description 108
- 238000001125 extrusion Methods 0.000 claims abstract description 48
- 238000004080 punching Methods 0.000 claims abstract description 47
- 239000000741 silica gel Substances 0.000 claims abstract description 29
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 29
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000012528 membrane Substances 0.000 claims abstract description 22
- 239000000523 sample Substances 0.000 claims description 34
- 239000007788 liquid Substances 0.000 claims description 28
- 238000003860 storage Methods 0.000 claims description 21
- 230000005540 biological transmission Effects 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 15
- 230000002401 inhibitory effect Effects 0.000 claims description 10
- 239000012472 biological sample Substances 0.000 claims description 9
- 230000000149 penetrating effect Effects 0.000 claims description 9
- 108010025899 gelatin film Proteins 0.000 claims description 6
- 230000004907 flux Effects 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000002493 microarray Methods 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000000016 photochemical curing Methods 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses an array biochip sample application device, which comprises a spray head, an array spray hole component arranged in the spray head and a power component arranged above the spray head and used for applying downward impact force to the array spray hole component, wherein the spray hole component comprises a spray head body and a spray head body; the array spray hole assembly comprises an array spray hole module, a silica gel membrane, a membrane fastening plate, an array punching needle and other structures; the array spray hole module comprises a plurality of extrusion cavities which are arranged in an array manner, and spray holes are arranged at the lower part of the extrusion cavities; can meet the simultaneous sample application of a plurality of spray holes and the requirements of high-flux and modularized sample application.
Description
Technical Field
The invention belongs to the technical field of biological instruments and equipment, and particularly relates to an array biochip sample application device.
Background
The sample application head adopted by the sample application instrument in the market at present mainly comprises a contact sample application needle, the contact sample application has higher risk of reagent cross contamination under the repeated sample application condition, and the sample application needle has low vulnerability and low preparation efficiency of the microarray chip. For non-contact sample application, the sample application process can independently solve the problems of cross contamination of contact sample application, damage of the sample application needle to the substrate and the like. The existing non-contact sample application spray heads on the market have low sample application efficiency, and a series of processes such as repeatedly cleaning the spray heads and drying are needed when different biological samples are applied, so that the problems of long time consumption, complex device, high manufacturing cost, inconvenience in non-modular assembly and disassembly and the like are caused.
Disclosure of Invention
Aiming at the technical problems existing in the prior art, the invention aims to provide a microarray biochip sample application device which has simple structure, lower manufacturing cost, modular disassembly and high sample application efficiency and can realize the large-area and high-density manufacturing of microarray chips.
In order to achieve the above purpose, the invention adopts the following technical scheme:
An array biochip sample application device comprises a spray head, an array spray hole component arranged in the spray head, and a power component arranged above the spray head and used for applying downward impact force to the array spray hole component; wherein:
The spray head comprises a lower cover plate and an upper cover plate for covering the lower cover plate, a plurality of first threaded holes are correspondingly formed in the peripheral sides of the lower cover plate and the upper cover plate, and the lower cover plate and the upper cover plate are fixedly connected through bolts penetrating through the first threaded holes; the middle part of the lower cover plate is provided with a first cavity for accommodating the array spray hole assembly, and the middle part of the first cavity is a through nozzle through hole; the middle part of the upper cover plate is provided with a through guide hole; a force transmission positioning column is arranged in the guide hole and is positioned under the bottom of the power assembly, so that the phenomenon of uneven impact force due to deviation of impact positions when the power assembly directly impacts the array spray hole assembly can be avoided; further, a second cavity matched with the shape of the array punching needle is formed in the middle of the bottom surface of the upper cover plate and used for limiting the position of the array punching needle and keeping the stability of the position of the array punching needle; the guide hole is located at the middle part of the upper part of the second cavity and is communicated with the second cavity.
The array spray hole assembly comprises an array spray hole module, a silica gel membrane, a membrane fastening plate and an array punching needle; the array spray hole module comprises a spray hole base block, the middle part of the spray hole base block is inwards sunken to form a spray hole cavity body, the middle part of the spray hole cavity body is provided with a plurality of extrusion cavities which are arranged in an array, and the middle part of each extrusion cavity is a spray hole penetrating through the spray hole base block; furthermore, the number of extrusion cavities in the array spray hole module is 1-48, the interval between two adjacent spray holes is 0.1-5 mm, the design of a plurality of spray holes can improve the sample application efficiency, and the high-flux and modularized sample application requirements are met. The outer side of the extrusion cavity and the periphery side of the jet orifice base block are provided with a plurality of liquid storage cavities for storing biological samples, the number of the liquid storage cavities is the same as that of the extrusion cavities, each liquid storage cavity is communicated with the extrusion cavity through a flow channel arranged in the jet orifice base block, and sample injection can be carried out between the liquid storage cavity and the extrusion cavity through capillary force; in order to prevent biological sample from flowing back to the liquid storage cavity in the sample application process, one end of the runner, which is close to the extrusion cavity, is provided with a flow inhibiting structure, the flow inhibiting structure is in a boss shape, the flow inhibiting structure reduces the liquid flux of the runner, which is close to the position of the liquid storage cavity, and reduces the liquid in the extrusion cavity from flowing back to the liquid storage cavity along the runner in the process of extruding the silica gel diaphragm through the punching needle. The silica gel membrane is positioned right above the extrusion cavity and is used for sealing the extrusion cavity; the array punching needle is positioned right above the silica gel membrane, the bottom of the array punching needle is provided with punching needles corresponding to the extrusion cavities one by one, the punching needles can extrude the silica gel membrane under the downward action generated by the power assembly, and then biological samples in the extrusion cavities are ejected out from the spray holes in the form of liquid drops, so that sample application is completed. The positioning groove is formed in the middle of the top surface of the array punching needle, the shape of the positioning groove is matched with the shape of the bottom of the force transmission positioning column, the acting force point of the force transmission positioning column can be located in the middle of the array punching needle through the design of the positioning groove, and the uniformity of the stress of the array punching needle is guaranteed while the positioning accuracy is improved.
The film fastening plate is used for pressing the silica gel film so as to seal the extrusion cavity in the array spray hole module, and is positioned between the array punching needle and the silica gel film; limiting holes corresponding to the punching heads one by one are formed in the middle of the diaphragm fastening plate, and the punching heads are in clearance fit with the limiting holes and can penetrate through the limiting holes to extrude the silica gel diaphragm; the length of the punching needle head is 0.2-1 mm longer than the depth of the limit hole. Further, the longitudinal section of the diaphragm fastening plate is T-shaped and comprises a clamping part positioned at the upper part and a limiting part positioned at the lower part; the size of the limiting part is matched with the size of the spray hole cavity; a clamping table is formed between the spray hole cavity and the spray hole base block, and the size of the clamping table is matched with the size of the area of the clamping part beyond the limiting part.
Further, the power component is an electromagnetic power component and comprises an electromagnet shell and an electromagnet core, wherein the electromagnet shell and the electromagnet core are arranged above the spray head, a supporting seat for fixing the electromagnet shell is arranged at the top of the upper cover plate, a second threaded hole is formed in the side wall of the supporting seat along the horizontal direction, and the electromagnet shell is fixed inside the supporting seat through a bolt penetrating through the second threaded hole. When the electromagnet core moves downwards, the electromagnet core can apply downward acting force to the force transmission positioning column, and the array punching needle is synchronously driven to move in the downward movement process of the force transmission positioning column. In addition, the power component can also be replaced by a piezoelectric column as a power source, and downward impact force can be generated by the piezoelectric column, so that the extrusion of the array spray hole component is realized, and the sample application process is completed.
Further, the array spray hole module, the diaphragm fastening plate, the array punching needle and the force conduction positioning column are processed by photo-curing of photosensitive resin materials, and the lower cover plate and the upper cover plate in the spray head are formed by laser processing of PMMA materials.
Compared with the prior art, the invention has the beneficial effects that:
The array spray hole module in the array spray hole assembly comprises a plurality of extrusion cavities which are arranged in an array, spray holes are arranged in the middle of the extrusion cavities, namely, the spray holes are arranged in an array, each spray hole in the sample application device provided by the invention is independently injected, so that different samples can be added to different liquid storage cavities in a spray head while the pollution-free and high-precision requirements are met, the simultaneous injection sample application of multiple samples is realized, the time for cleaning, drying and re-sample application required by sample changing in the traditional sample application process is reduced, and the preparation efficiency of a biochip is improved; can meet the simultaneous sample application of a plurality of spray holes and the requirements of high-flux and modularized sample application. In addition, the liquid storage cavity and the extrusion cavity in the device provided by the invention are injected by capillary force, an external injection power source is not needed, the injection is stable, the device is small and exquisite, the design and the processing are simple, the manufacturing cost is low, and the integration and the scale are easy.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of an array biochip spotting apparatus according to the present invention;
FIG. 2 is a cross-sectional view of FIG. 1;
FIG. 3 is a schematic diagram of the explosive structure of FIG. 1;
FIG. 4 is a schematic diagram of an array nozzle module;
Reference numerals: the device comprises a 1-spray head, a 11-lower cover plate, a 12-upper cover plate, a 13-first threaded hole, a 14-first cavity, a 15-spray head through hole, a 16-guide hole, a 17-second cavity, a 21-array spray hole module, a 211-spray hole base block, a 212-spray hole cavity, a 213-extrusion cavity, a 214-spray hole, a 215-liquid storage cavity, a 216-flow channel, a 217-flow inhibiting structure, a 22-silica gel membrane, a 23-membrane fastening plate, a 231-limiting hole, a 232-clamping part, a 233-limiting part, a 24-array punching needle, a 241-punching needle, a 242-positioning groove, a 25-clamping table, a 3-power assembly, a 31-electromagnet shell, a 32-electromagnet core, a 4-force conduction positioning column, a 5-supporting seat, a 6-second threaded hole and a 7-biological sample.
Detailed Description
The present invention will be further described with reference to examples, which are not intended to be limiting, so that those skilled in the art will better understand the present invention and practice it.
In the description of the present invention, it should be understood that the directions or positional relationships indicated by the terms "upper", "lower", "top", "bottom", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limitations of the present invention. The terms "first" and "second" in this specification do not denote a particular quantity or order, but rather are used for distinguishing between similar or identical items.
In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and include, for example, either permanently connected, removably connected, or integrally connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
Referring to fig. 1 to 4, an array type biochip spotting apparatus includes a spray head 1, an array spray hole assembly installed inside the spray head 1, and a power assembly 3 installed above the spray head 1 for applying a downward impact force to the array spray hole assembly; wherein:
The spray head 1 comprises a lower cover plate 11 and an upper cover plate 12 for covering the lower cover plate, a plurality of first threaded holes 13 are correspondingly formed in the peripheral sides of the lower cover plate 11 and the upper cover plate 12, and the lower cover plate 11 and the upper cover plate 12 are fixedly connected through bolts penetrating through the first threaded holes 13; the middle part of the lower cover plate 11 is provided with a first cavity 14 for accommodating the array spray hole assembly, and the middle part of the first cavity 14 is provided with a through spray head through hole 15; the middle part of the upper cover plate 12 is provided with a through guide hole 16; the force transmission positioning column 4 is arranged in the guide hole 16, the force transmission positioning column 4 is positioned under the bottom of the power assembly 3, and the force transmission positioning column 4 can avoid the phenomena of impact position deviation and uneven impact force when the power assembly 3 directly arrays the spray hole assembly; further, the middle part of the bottom surface of the upper cover plate 12 is provided with a second cavity 17 which is matched with the shape of the array punch pin 24, and the second cavity 17 is used for limiting the position of the array punch pin 24; the guide hole 16 is located at the upper middle part of the second cavity 17 and communicates with the second cavity 17.
The array spray hole assembly comprises an array spray hole module 21, a silica gel membrane 22, a membrane fastening plate 23 and an array punching needle 24; the array spray hole module 21 comprises a spray hole base block 211, the middle part of the spray hole base block 211 is inwards sunken to form a spray hole cavity 212, the middle part of the spray hole cavity 212 is provided with a plurality of extrusion cavities 213 which are arranged in an array, and the middle part of the extrusion cavities 213 is a spray hole 214 penetrating through the spray hole base block; further, the number of extrusion chambers 213 in the array nozzle module is 1-48, and the interval between two adjacent nozzles 214 is 0.1-5 mm. The outer side of the extrusion cavity 213 and the periphery side of the jet orifice base block 211 are provided with a plurality of liquid storage cavities 215 for storing biological samples 7, the number of the liquid storage cavities 215 is the same as that of the extrusion cavities 213, and each liquid storage cavity 215 is communicated with the extrusion cavity 213 through a flow channel 216 arranged in the jet orifice base block; in order to prevent the biological sample from flowing back into the liquid storage cavity 215 during the spotting process, the flow channel 216 is provided with a flow inhibiting structure 217 at one end close to the extrusion cavity 213, the flow inhibiting structure 217 is in a boss shape, and the flow inhibiting structure reduces the liquid flux of the flow channel at the position close to the liquid storage cavity. The silica gel membrane 22 is located right above the extrusion cavity 213 and is used for sealing the extrusion cavity 213; the array punching needle 24 is positioned right above the silica gel membrane 22, the bottom of the array punching needle 24 is provided with punching needles 241 which are in one-to-one correspondence with the extrusion cavities 213, and the punching needles 241 can extrude the silica gel membrane under the downward action generated by the power assembly; the middle part of the top surface of the array punching needle 24 is provided with a positioning groove 242, the shape of the positioning groove 242 is matched with the shape of the bottom of the force transmission positioning column 4, and the acting force point of the force transmission positioning column 4 can be positioned in the middle part of the array punching needle 24 through the positioning groove 242, so that the uniformity of the stress of the array punching needle is improved.
The film fastening plate 23 is used for pressing the silica gel film 22 to seal the extrusion cavity 213 inside the array spray hole module, and is positioned between the array punch pin 24 and the silica gel film 22; limiting holes 231 which are in one-to-one correspondence with the punching heads 241 are formed in the middle of the diaphragm fastening plate 23, and the punching heads 241 are in clearance fit with the limiting holes 231 and can penetrate through the limiting holes to extrude the silica gel diaphragm; the length of the punching needle 241 is 0.2-1 mm longer than the depth of the limiting hole 231 to ensure that the punching needle can penetrate through the limiting hole and extrude the silica gel membrane in the extrusion process. Further, the longitudinal section of the film fastening plate 23 is T-shaped, and includes a clamping portion 232 at the upper portion and a limiting portion 233 at the lower portion; the size of the limit part 233 is matched with the size of the spray hole cavity 212; a clamping table 25 is formed between the spray hole cavity 212 and the spray hole base block 211, the size of the clamping table 25 is matched with the size of the area of the clamping part beyond the limiting part, and the diaphragm fastening plate 23 is clamped on the clamping table 25 through the clamping part 232, so that the diaphragm fastening plate 23 is limited in the spray hole cavity 212 in the array spray hole module.
In one embodiment, the power component 3 is an electromagnetic power component, and comprises an electromagnet shell 31 and an electromagnet core 32 which are arranged above the spray head, a supporting seat 5 for fixing the electromagnet shell is arranged at the top of the upper cover plate 12, a second threaded hole 6 is formed in the side wall of the supporting seat 5 along the horizontal direction, and the electromagnet shell 31 is fixed in the supporting seat 5 through a bolt penetrating through the second threaded hole 6, so that the impact height of the electromagnet can be conveniently adjusted. When the electromagnet core 31 moves downwards, the electromagnet core can apply downward acting force to the force transmission positioning column 4, and the array punching needle 24 is synchronously driven to move in the downward movement process of the force transmission positioning column 4. It should be noted that the power component can also be replaced by a piezoelectric column as a power source, which can generate downward impact force, so as to squeeze the array spray hole component and complete the sample application process. Those skilled in the art can select a suitable power source as the electromagnetic power component according to the implementation needs, and the electromagnetic power component can be applied to the scheme as long as the electromagnetic power component can generate downward impact force.
The sample application device provided by the invention has the working procedures that:
When the electromagnet is at a high level, the electromagnet iron core 32 impacts the force transmission positioning column 4 downwards, the force transmission positioning column 4 impacts the silica gel membrane 22, the silica gel membrane 22 deforms downwards, the pressure of the extrusion cavity 213 increases suddenly, and the reagent (biological sample) to be spotted in the extrusion cavity is ejected from the spray hole 214 in a liquid drop shape; when the electromagnet is at a low level, the electromagnet core 32 is lifted upwards, the silica gel membrane 22 is deformed and restored, the pressure of the extrusion cavity 213 is suddenly reduced, and the reagent to be spotted in the liquid storage cavity based on the capillary sampling principle is filled into the extrusion cavity 213, so that a complete injection process is realized; repeating the steps to perform subsequent sample application.
It will be apparent that the described embodiments are some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Claims (10)
1. An array biochip sample application device, which is characterized in that: the device comprises a spray head, an array spray hole assembly arranged in the spray head, and a power assembly arranged above the spray head and used for applying downward impact force to the array spray hole assembly; wherein:
the spray head comprises a lower cover plate and an upper cover plate for covering the lower cover plate, a first cavity for accommodating the array spray hole assembly is arranged in the middle of the lower cover plate, and a through spray head through hole is formed in the middle of the first cavity; the middle part of the upper cover plate is provided with a through guide hole; a force transmission positioning column is arranged in the guide hole and is positioned right below the bottom of the power assembly;
The array spray hole assembly comprises an array spray hole module, a silica gel membrane and an array punching needle; the array spray hole module comprises a spray hole base block, the middle part of the spray hole base block is inwards recessed to form a spray hole cavity, the middle part of the spray hole cavity is provided with a plurality of extrusion cavities which are arranged in an array, and the middle part of each extrusion cavity is a spray hole penetrating through the spray hole base block; the outer side of the extrusion cavity and the periphery side of the jet orifice base block are provided with a plurality of liquid storage cavities for storing biological samples, the number of the liquid storage cavities is the same as that of the extrusion cavities, and each liquid storage cavity is communicated with the extrusion cavity through a flow channel arranged in the jet orifice base block; the silica gel membrane is positioned right above the extrusion cavity and is used for sealing the extrusion cavity; the array punching needle is positioned right above the silica gel membrane, the bottom of the array punching needle is provided with punching needles corresponding to the extrusion cavities one by one, and the punching needles can extrude the silica gel membrane under the downward action generated by the power assembly.
2. The array biochip spotting device of claim 1, wherein: the array spray hole assembly further comprises a film fastening plate used for pressing the silica gel film, and the film fastening plate is positioned between the array punching needle and the silica gel film; limiting holes corresponding to the punching heads one by one are formed in the middle of the diaphragm fastening plate, and the punching heads are in clearance fit with the limiting holes and can penetrate through the limiting holes to extrude the silica gel diaphragm; the length of the punching needle head is 0.2-1 mm longer than the depth of the limiting hole.
3. The array biochip spotting device of claim 2, characterized in that: the longitudinal section of the diaphragm fastening plate is T-shaped and comprises a clamping part positioned at the upper part and a limiting part positioned at the lower part; the size of the limiting part is matched with the size of the spray hole cavity; and a clamping table is formed between the spray hole cavity and the spray hole base block, and the size of the clamping table is matched with the size of the area of the clamping part beyond the limiting part.
4. The array biochip spotting device of claim 1, wherein: the middle part of the bottom surface of the upper cover plate is provided with a second cavity matched with the shape of the array punching needle, and the guide hole is positioned at the middle part of the upper part of the second cavity and is communicated with the second cavity.
5. The array biochip spotting device of claim 4, wherein: the middle part of the top surface of the array punching needle is provided with a positioning groove, and the shape of the positioning groove is matched with the shape of the bottom of the force transmission positioning column.
6. The array biochip spotting device of claim 1, wherein: the number of extrusion cavities in the array spray hole module is 1-48, and the interval between two adjacent spray holes is 0.1-5 mm.
7. The array biochip spotting device of claim 1, wherein: the periphery of the lower cover plate and the periphery of the upper cover plate are correspondingly provided with a plurality of first threaded holes, and the lower cover plate and the upper cover plate are fixedly connected through bolts penetrating through the first threaded holes.
8. The array biochip spotting device according to any one of claims 1 to 7, characterized in that: the flow channel is provided with a flow inhibiting structure at one end close to the extrusion cavity, the flow inhibiting structure is in a boss shape, the flow inhibiting structure reduces the liquid flux of the position of the flow channel close to the liquid storage cavity, and reduces the liquid in the extrusion cavity from flowing back to the liquid storage cavity along the flow channel in the process of extruding the silica gel diaphragm by the punching needle.
9. The array biochip spotting device of claim 8, wherein: the power component is an electromagnetic power component and comprises an electromagnet shell and an electromagnet core, wherein the electromagnet shell and the electromagnet core are arranged above the spray head, the electromagnet core can apply downward acting force to the force transmission positioning column when moving downwards, and the array punching needle is synchronously driven to move in the downward movement process of the force transmission positioning column.
10. The array biochip spotting device of claim 9, characterized in that: the supporting seat for fixing the electromagnet shell is arranged at the top of the upper cover plate, the second threaded hole is formed in the side wall of the supporting seat along the horizontal direction, and the electromagnet shell is fixed inside the supporting seat through a bolt penetrating through the second threaded hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202211066755.2A CN115452507B (en) | 2022-09-01 | 2022-09-01 | Array biochip sample application device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211066755.2A CN115452507B (en) | 2022-09-01 | 2022-09-01 | Array biochip sample application device |
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| CN202705345U (en) * | 2012-05-28 | 2013-01-30 | 上海理工大学 | Microarray biochip preparation device |
| KR20180038743A (en) * | 2016-10-07 | 2018-04-17 | 주식회사 제타 | Bio-Chip Arrayer |
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2022
- 2022-09-01 CN CN202211066755.2A patent/CN115452507B/en active Active
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|---|---|---|---|---|
| CN102649931A (en) * | 2012-05-28 | 2012-08-29 | 上海理工大学 | Preparation method for microarray biochip |
| CN202705345U (en) * | 2012-05-28 | 2013-01-30 | 上海理工大学 | Microarray biochip preparation device |
| KR20180038743A (en) * | 2016-10-07 | 2018-04-17 | 주식회사 제타 | Bio-Chip Arrayer |
| KR102078567B1 (en) * | 2018-10-01 | 2020-02-19 | 주식회사 이바이오젠 | Non-contact microarrayer using image recognition technology |
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| Title |
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| CN115452507A (en) | 2022-12-09 |
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