KR20180038743A - Bio-Chip Arrayer - Google Patents

Abstract

Disclosed is a biochip arrayer. The biochip arrayer comprises: a spotting unit comprising at least one spotting pin for spotting a biological sample on a solid substrate; a dispensing unit including at least one dispensing nozzle for dispensing the sample in a form of liquid droplets on the solid substrate; a holder to which the spotting unit and the dispensing unit are selectively mounted; a vacuum chuck comprising a porous panel and supporting the solid substrate; and a driving unit for moving the holder in horizontal and vertical directions for spotting and dispensing the sample.

Description

Bio-Chip Arrayer {Bio-Chip Arrayer}

Embodiments of the present invention relate to biochip layers. More particularly, the present invention relates to a bio chip layer capable of both a contact method using a spotting pin and a non-contact type bio chip array process using a dispensing nozzle.

A biochip is a biological array in which a biological material such as a nucleic acid is immobilized on a substrate. For example, a bio-sample such as protein, DNA, or RNA is immobilized on a substrate. The basic principle of the biochip is based on the interaction of a target molecule with a fixed molecule immobilized on a substrate. For example, in the case of a DNA chip, it is based on the complementary binding between the oligonucleotides immobilized on a substrate and the DNA base present in the sample. In the case of protein chips, it is based on the interaction between protein molecules such as antigen-antibody binding or ligand-receptor binding.

The biochip layer can be used for the production of DNA chips and RNA chips for genetic research and for the production of protein chips for constructing disease diagnosis systems. For example, the biochip layer may be formed by a pin method (contact method) in which a bio sample is fixed on a substrate using a spotting pin according to a method of fixing the bio sample on a solid substrate, A photolithography method in which a bio sample is synthesized directly on a substrate using a photosensitive material, a method in which a substance having a positive charge is applied to a specific position on the substrate, And a method of attaching a bio-sample having a specific surface area. As an example, U.S. Patent No. 7,335,338 discloses a micro array spotting apparatus using a plurality of pins, and U.S. Patent No. 7,736,591 discloses an ink jet array apparatus using a plurality of micro dispensers.

On the other hand, in the case of a DNA chip for genetic research, the pinch-type biochip layer is generally used, but in the case of a protein chip, unlike a DNA chip, a reaction between an antigen and a primary antibody, a reaction between a primary antibody and a secondary antibody And the like. Further, for the purpose of binding to the antibody, the structure of the site showing antigenicity must be fixed to the substrate in a state that the structure is not deformed. For this reason, the protein sample may be dispensed in droplets An ink jet type biochip layer may be used.

Embodiments of the present invention provide a bio chip layer capable of both a contact method using a spotting pin and a non-contact type bio chip array process using a dispensing nozzle.

A biochip layer in accordance with embodiments of the present invention includes a spotting unit comprising at least one spotting pin for spotting a biological sample on a solid substrate and a spotting unit for forming the sample in droplet form A holder for selectively mounting the spotting unit and the dispensing unit; a vacuum chuck for supporting the solid substrate and including a porous panel; And a driving unit for moving the holder in the horizontal and vertical directions for spotting and dispensing the sample.

According to embodiments of the present invention, the spotting unit and the dispensing unit each include a first body and a second body on which the spotting pin and the dispensing nozzle are mounted, and the first body and the second body A hook member for mounting the holder on the rear surface of the body is provided, and a hook groove into which the hook member is inserted may be provided on the front surface of the holder.

According to embodiments of the present invention, the vacuum chuck includes a chuck body connected to a vacuum pump, and the porous panel may be disposed on the chuck body so that vacuum pressure is applied to the porous panel from the vacuum pump.

According to embodiments of the present invention, the solid substrate, for example, a membrane pad, may be disposed on the porous panel.

According to embodiments of the present invention, the vacuum chuck further comprises a support panel vacuum-adsorbed on the porous panel, and as the solid substrate, for example, a slide glass may be disposed on the support panel.

According to embodiments of the present invention, the chuck body may be provided with a cooling water channel for controlling the temperature.

According to embodiments of the present invention, the biochip layer may further include a support unit for supporting at least one well plate in which the sample is accommodated.

According to embodiments of the present invention, the biochip layer may further include a cleaning unit for cleaning the spotting pin and the dispensing nozzle.

According to the embodiments of the present invention, the cleaning unit includes a cleaning tank to which a washer fluid for cleaning the spotting pin and the dispensing nozzle is supplied, a vacuum port for removing the sample remaining on the spotting pin, And a drain tank for discharging the sample remaining in the dispensing nozzle.

According to the embodiments of the present invention, a channel for overflowing the washer fluid supplied to the washing tank to the drain tank may be provided between the washing tank and the drain tank.

According to embodiments of the present invention, the biochip layer may include a sample tube for receiving droplets ejected from the dispensing nozzle for ejection test of the sample by the dispensing nozzle, and a spot by the spotting pin And a sample block for supporting a sample substrate for a spotting test for measuring the size of the sample substrate.

According to embodiments of the present invention, a first measurement camera for measuring a spot size formed on the sample substrate may be mounted on one side of the holder.

According to embodiments of the present invention, the biochip layer may further include a second measurement camera for measuring a size of a droplet discharged from the dispensing nozzle.

According to embodiments of the present invention, the biochip layer includes a process chamber for performing a biochip array process using the spotting unit and the dispensing unit, a humidity sensor for controlling the humidity inside the process chamber, And may further comprise an adjustment unit.

According to embodiments of the present invention, the humidity control unit includes a humidifier, a fan filter unit for providing air humidified by the humidifier to the process chamber, a humidity sensor for measuring humidity inside the process chamber, . ≪ / RTI >

According to embodiments of the present invention as described above, the biochip layer includes a spotting unit for spotting a sample on a solid substrate such as a slide glass, a membrane pad, etc., and a spotting unit for spotting the sample in a droplet form And the spotting unit and the dispensing unit may be selectively used as needed. Therefore, both the contact type array process using the spotting pins and the non-contact type array process using the dispensing nozzle can be performed using the biochip layer, so that the cost for research and manufacture of the biochip is large Can be saved.

In addition, the biochip layer may include a vacuum chuck for supporting the solid substrate. The vacuum chuck can include a porous panel, and can reliably support a flexible solid substrate such as a membrane pad using the vacuum pressure provided through the porous panel. Particularly, in order to support a solid substrate such as a slide glass, a support panel or white board composed of white can be vacuum-adsorbed on the porous panel, and observation such as measurement of the size of spots formed on the slide glass is very easy Lt; / RTI >

In addition, the biochip layer may include a humidity control unit that can provide humidified air with moisture particles removed into the process chamber, thereby preventing condensation in the process chamber.

BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a schematic diagram for explaining a biochip layer according to an embodiment of the present invention; FIG.
Fig. 2 is a schematic front view for explaining the spotting unit shown in Fig. 1. Fig.
Fig. 3 is a schematic side view for explaining the spotting unit shown in Fig. 1. Fig.
4 is a schematic plan view for explaining the spotting unit shown in Fig.
5 is a schematic front view for explaining the holder shown in Fig.
6 is a schematic front view for explaining the dispensing unit shown in Fig.
7 is a schematic plan view for explaining the vacuum chuck shown in Fig.
8 is a schematic sectional view for explaining the vacuum chuck shown in Fig.
9 is a schematic cross-sectional view for explaining another example of the vacuum chuck shown in Fig.
10 is a schematic plan view for explaining the support panel shown in Fig.
11 is a schematic plan view for explaining the support unit shown in Fig.
12 is a schematic plan view for explaining the cleaning unit shown in Fig.
FIG. 13 is a schematic plan view for explaining the second measurement camera and the measurement bed shown in FIG. 1; FIG.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, the present invention should not be construed as limited to the embodiments described below, but may be embodied in various other forms. The following examples are provided so that those skilled in the art can fully understand the scope of the present invention, rather than being provided so as to enable the present invention to be fully completed.

In the embodiments of the present invention, when one element is described as being placed on or connected to another element, the element may be disposed or connected directly to the other element, . Alternatively, if one element is described as being placed directly on another element or connected, there can be no other element between them. The terms first, second, third, etc. may be used to describe various items such as various elements, compositions, regions, layers and / or portions, but the items are not limited by these terms .

The terminology used in the embodiments of the present invention is used for the purpose of describing specific embodiments only, and is not intended to be limiting of the present invention. Furthermore, all terms including technical and scientific terms have the same meaning as will be understood by those skilled in the art having ordinary skill in the art, unless otherwise specified. These terms, such as those defined in conventional dictionaries, shall be construed to have meanings consistent with their meanings in the context of the related art and the description of the present invention, and are to be interpreted as being ideally or externally grossly intuitive It will not be interpreted.

Embodiments of the present invention are described with reference to schematic illustrations of ideal embodiments of the present invention. Accordingly, changes from the shapes of the illustrations, e.g., changes in manufacturing methods and / or tolerances, are those that can be reasonably expected. Accordingly, the embodiments of the present invention should not be construed as being limited to the specific shapes of the regions described in the drawings, but include deviations in the shapes, and the elements described in the drawings are entirely schematic and their shapes Is not intended to describe the exact shape of the elements and is not intended to limit the scope of the invention.

BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a schematic diagram for explaining a biochip layer according to an embodiment of the present invention; FIG.

1, a biochip layer 100 according to an embodiment of the present invention can be used for research and manufacture of biochips such as a DNA chip, an RNA chip, and a protein chip for genetic research and disease diagnosis systems . In particular, the biochip layer 100 according to an embodiment of the present invention can be configured to be capable of both a contact method and a non-contact type biochip array process.

According to one embodiment of the present invention, the biochip layer 100 includes a spotting pin 112 (see FIG. 2) including at least one spotting pin 112 (see FIG. 2) for spotting a bio- Unit 110 and a dispensing unit 120 that includes at least one dispensing nozzle 122 (see FIG. 6) for dispensing the sample in the form of a microdroplet on the solid substrate. In particular, the biochip layer 100 may include a holder 130 for selectively mounting the spotting unit 110 and the dispensing unit 120, if necessary. For example, the spotting unit 110 may be used when performing a DNA chip or an RNA chip array process, and the dispensing unit 120 may be used when performing a protein chip array process. The spotting unit 110 and the dispensing unit 120 are connected to the first body 114 (see FIG. 2) in which the spotting pin 112 and the dispensing nozzle 122 are mounted, and the second body 116 ; See FIG. 6).

Fig. 2 is a schematic front view for explaining the spotting unit shown in Fig. 1, Fig. 3 is a schematic side view for explaining the spotting unit shown in Fig. 1, Fig. 4 is a cross- Fig. 3 is a schematic plan view for explaining a unit. Fig. 5 is a schematic front view for explaining the holder shown in Fig.

2 to 5, a plurality of spotting pins 112 may be mounted on the front surface of the first body 114 of the spotting unit 110. For example, a plurality of through holes through which the spotting pins 112 are inserted in the vertical direction may be provided on the front surface of the first body 114. Although not shown in detail, each of the spotting pins 112 May be provided with an upper head and movable in the vertical direction within the through holes. Also, although not shown, a slit may be formed at the lower end of each of the spotting pins 112 in a vertical direction similar to a pen tip, and the sample may be accommodated in the slot by capillary phenomenon.

In the meantime, although eight spotting pins 112 are mounted on the front surface of the first body 114, the number of the spotting pins 112 can be variously changed, It will not be limited.

A hook member 116 for coupling with the holder 130 may be provided on a rear surface of the first body 114. A hook groove 116 into which the hook member 116 is inserted may be formed on a front surface of the holder 130 134 may be provided.

A mounting channel 132 in which the first body 114 is inserted is vertically provided on a front surface of the holder 130 and the hook groove 134 is formed on a bottom surface of the mounting channel 132. [ . In particular, the hook member 116 may have a substantially rectangular block shape and may be connected to the rear surface of the first body 114 through the connecting member 118. The hook groove 134 has a depth such that the rear surface of the first body 114 can be brought into close contact with the bottom surface of the mounting channel 132. The hook groove 134 has a pocket shape for downward movement of the hook member 116. [ As shown in Fig. At this time, a slot 136 through which the connecting member 118 passes may be provided on the bottom surface of the mounting channel 132 so that the hook member 116 can be moved downward.

In addition, the first body 114 may have a width that gradually narrows downward as shown, and the mounting channel 132 also has a width that gradually narrows downwardly corresponding to the first body 114 . As a result, the outer surfaces of the first body 114 can be in close contact with the inner surfaces of the mounting channel 132, whereby the spotting unit 110 can be stably mounted on the holder 130 .

6 is a schematic front view for explaining the dispensing unit shown in Fig.

Referring to FIG. 6, the dispensing unit 120 may include a second body 124 having a plurality of dispensing nozzles 122 mounted thereon. Although four dispensing nozzles 122 are mounted on the front portion of the second body 124, the number of the dispensing nozzles 122 can be variously changed as needed. The scope of the present invention is not limited thereto.

As one example, piezo-electric dispensers may be used as the dispensing nozzles 122. Although not shown in detail, the piezoelectric dispenser may include a glass capillary tube connected to a piezoelectric ceramic tube, and the piezoelectric ceramic tube may have electrodes to which an electrical pulse signal is applied. Further, the piezoelectric ceramic tube may be connected to a pump, for example, a syringe pump through a soft piping, and the pump may be used to suck or discharge the sample to the glass capillary.

Although not shown, the second body 124 of the dispensing unit 120 may have substantially the same shape as that of the first body 114, A hook member (not shown) identical to the spotting unit 110 may be provided. Accordingly, the spotting unit 110 and the dispensing unit 120 can be selectively mounted on the holder 130 as needed.

Referring again to FIG. 1, the biochip layer 100 may include a base plate 102 and a vacuum chuck 150 disposed on the base plate 102 and supporting a solid substrate. As the solid substrate, a substrate such as a slide glass 10 (see FIG. 10) for manufacturing a biochip, a membrane pad (not shown) for manufacturing a disease diagnosis kit, and the like may be used.

FIG. 7 is a schematic plan view for explaining the vacuum chuck shown in FIG. 1, and FIG. 8 is a schematic sectional view for explaining the vacuum chuck shown in FIG.

Referring to FIG. 7, according to an embodiment of the present invention, the vacuum chuck 150 includes a chuck body 152 having a substantially rectangular plate shape and disposed on the base plate 102, And a porous panel 154 for vacuum-adsorbing the solid substrate.

For example, the upper surface of the chuck body 152 may be provided with a recess into which the porous panel 154 is inserted. Vacuum holes (not shown) connected to a vacuum pump 156 may be provided. The porous panel 154 may be fabricated through a ceramic sintering process and the solid substrate, for example, the membrane pad (not shown), placed on the porous panel 154, And can be vacuum adsorbed onto the porous panel 154 by pneumatic pressure.

The chuck body 152 may include a cooling water channel 158 for controlling the temperature. For example, tertiary distilled water, which is supplied from a cooling water supply unit (not shown) through the cooling water flow path 158 can circulate through the vacuum chuck 150, thereby controlling the temperature of the vacuum chuck 150 to about 22 ° C. to 28 ° C. It can be kept constant.

FIG. 9 is a schematic sectional view for explaining another example of the vacuum chuck shown in FIG. 8, and FIG. 10 is a schematic plan view for explaining the support panel shown in FIG.

9 and 10, the vacuum chuck 150 may further include a support panel 160 vacuum-adsorbed on the porous panel 154, and the solid substrate may be supported on the support panel 160 As shown in FIG. For example, a plurality of slide glasses 10 used as the solid substrate may be disposed on the support panel 160. At this time, the support panel 160 is preferably made of white so as to facilitate observation of sample spots formed on the slide glasses 10.

Alignment pins 162 may be provided on the chuck body 152 and alignment grooves may be formed on the lower surface of the support panel 160 to receive the aligning pins 162.

Referring to FIG. 1 again, the bio chip layer 100 may include a driving unit 140 for moving the holder 130 horizontally and vertically for spotting and dispensing the sample. For example, a three-axis rectangular coordinate robot may be used as the driving unit 140, and the three-axis rectangular coordinate robot may be disposed on the base plate 102.

11 is a schematic plan view for explaining the support unit shown in Fig.

Referring to FIG. 11, a support unit 170 for supporting at least one well plate 20 accommodating the sample may be disposed at one side of the vacuum chuck 150. Although four well plates 20 are disposed on the support unit 170, the number of the well plates 20 can be variously changed as needed, so that the scope of the present invention is not limited thereto. It will not be limited.

For example, the support unit 170 may have a substantially square plate shape, and recesses 172 for inserting the well plates 20 may be provided on the upper surface of the support unit 170 . Although not shown, a cooling water channel (not shown) for temperature control may be provided in the support unit 170. For example, tertiary distilled water, which is supplied from a cooling water supply unit (not shown) through the cooling water flow path, through which the temperature of the support unit 170 is maintained at about 22 ° C. to 28 ° C. .

The driving unit 140 may move the holder 130 such that the end portions of the spotting pins 112 or the dispensing nozzles 122 are immersed in the sample contained in the well plate 20. [ The sample can be stuck to the slits of the spotting pins 112 by capillary phenomenon. Alternatively, in the case of the dispensing nozzles 122, the sample may be sucked into the dispensing nozzles 122 by the operation of the syringe pump. The driving unit 140 may move the spotting pins 112 or the dispensing nozzles 122 to the upper side of the vacuum chuck 150 and may then move the solid substrate supported by the vacuum chuck 150 That is, an array process for forming micro / nano spots on the slide glass 20 or the membrane pad (not shown) may be performed.

According to an embodiment of the present invention, the biochip layer 100 may include a cleaning unit 180 for cleaning the spotting pins 112 and the dispensing nozzles 122.

12 is a schematic plan view for explaining the cleaning unit shown in Fig.

Referring to FIG. 12, the cleaning unit 180 may be disposed on one side of the support unit 170 and may have a substantially rectangular block shape. The cleaning unit 180 includes a cleaning tank 182 to which a washer fluid for cleaning the spotting pins 112 and the dispensing nozzles 122 is supplied, Vacuum ports 184 for removing the sample, and a drainage tank 186 for discharging the sample remaining in the dispensing nozzles 122.

The washing tub 182 may be connected to a washer liquid supply unit (not shown) for supplying the washer liquid, and tertiary distilled water may be used as the washer liquid. The cleaning tank 182 may be formed on the cleaning unit 180 and the drain tank 186 may be formed on one side of the cleaning tank 182. In particular, a channel 188 may be provided between the cleaning tank 182 and the drain tank 186 to overflow the washer liquid supplied to the cleaning tank 182 to the drain tank 186, The washer fluid in the tank 186 may be discharged through a pipe to a discharge vessel (not shown).

For example, the driving unit 140 may move the holder 130 such that the dispensing nozzles 122 are positioned above the drain tank 186, and may be connected to the dispensing nozzles 122 Samples remaining in the dispensing nozzles 122 may be discharged to the drain tank 186 by syringe pumps. The driving unit 140 may move the holder 130 so that the end of the dispensing nozzles 122 may be immersed in the washer fluid received in the washing tub 182, Can be sucked into the dispensing nozzles 122. The washer fluid sucked into the dispensing nozzles 122 may be discharged to the drain tank 186 and the dispensing nozzles 122 may be sufficiently cleaned by repeating the suction and discharge of the washer liquid as described above .

The vacuum ports 184 may be connected to a vacuum pump (not shown) and may be used to remove residual samples from the spotting pins 112. For example, the driving unit 140 may move the holder 130 so that the spotting pins 112 are inserted into the vacuum ports 184, The sample can be removed from the spotting pins 112 by air pressure. The driving unit 140 may move the holder 130 so that the spotting pins 112 may be immersed in the washer fluid received in the washing tub 182. The residual sample can be sufficiently removed from the spotting pins 112 by repeatedly moving the spotting pins 112 between the vacuum ports 184 and the cleaning tank 182 as described above. In one example, the vacuum ports 184 may have an inner diameter of about 4 mm, and the spotting pins 112 may have a diameter of about 3 mm.

According to an embodiment of the present invention, the biochip layer 100 may measure the size of spots formed by the spotting pins 112 and the amount and size of droplets discharged from the dispensing nozzles 122 And a sample block 190 for measurement. For example, the sample block 190 may be provided with sample tubes (not shown) for receiving droplets discharged from the dispensing nozzles 122 for discharging the sample by the dispensing nozzles 122, And a recess 194 in which sample substrates 30 for spotting test for measuring the size of the spots by the spotting pins 112 are placed can be provided, May be provided.

For example, a PCR tube (not shown) may be inserted into the receiving holes 192, and the dispensing nozzles 122 may discharge droplets of predetermined number in the PCR tubes. After the sample is discharged to the PCR tubes as described above, the operation states of the dispensing nozzles 122 can be determined and the correction can be performed by measuring the weight of the PCR tubes.

In addition, sample spots formed by the spotting pins 112 may be formed on the sample substrates 30, and the spots may have a diameter of about 0.1 mm to 0.2 mm. According to an embodiment of the present invention, a first measurement camera 200 (see FIG. 1) for measuring the size of spots formed on the sample substrates 30 is mounted on one side of the holder 130 in a vertical direction .

The cleaning unit 180 and the sample block 190 may be integrally provided as a separate member, but the cleaning unit 180 and the sample block 190 may be provided as separate members have.

FIG. 13 is a schematic plan view for explaining the second measurement camera and the measurement bed shown in FIG. 1; FIG.

13, the biochip layer 100 includes a second measurement camera 210 for measuring the size of droplets discharged from the dispensing nozzles 122, . For example, a sample bed 212 on which a sample substrate 40 is placed may be provided on one side of the cleaning unit 180, and the second measurement camera 210 may be provided on one side of the sample bed 212 And can be arranged in the horizontal direction.

For example, the dispensing nozzles 122 may be located above the sample bed 212 and may be dispensed from one or a predetermined number of dispensing nozzles 122 onto the sample substrate 40, The droplet (s) can be ejected. The second measurement camera 210 can capture droplets ejected from the dispensing nozzles 122, and can calculate the diameter and volume of the droplets. As an example, the droplets discharged from the dispensing nozzles 122 may be about 0.5 to 50 nanometers.

Referring again to FIG. 1, the diode chip layer 100 includes a process chamber 104 for providing a space for performing an array process by the spotting pins 112 or the dispensing nozzles 122, And a humidity control unit 220 for controlling the humidity inside the process chamber 104. For example, although not shown in detail, the humidity control unit 220 includes a fan filter unit (not shown) for providing an ultrasonic or heated humidifier (not shown) and air humidified by the humidifier into the process chamber 104 And a humidity sensor (not shown) for measuring the humidity inside the process chamber 104. The humidity inside the process chamber 104 may be approximately 75 It can be adjusted to about ± 3%. Particularly, since the humidified air is supplied through the fan filter unit, the moisture particles in the air can be removed by the filter, thereby preventing the condensation inside the process chamber 104.

According to the embodiments of the present invention as described above, the biochip layer 100 includes a spotting unit 110 for spotting a sample on a solid substrate such as a slide glass 10, a membrane pad, And a dispensing nozzle 120 for discharging the sample in the form of droplets on a solid substrate. The spotting unit 110 and the dispensing unit 120 may be selectively used as needed. Therefore, both the contact type array process using the spotting pin 112 and the non-contact type array process using the dispensing nozzle 122 can be performed using the biochip layer 100, The cost of research and manufacturing can be greatly reduced.

In addition, the biochip layer 100 may include a vacuum chuck 150 for supporting the solid substrate. The vacuum chuck 150 may include a porous panel 154 and may support a flexible solid substrate such as a membrane pad using vacuum pressure provided through the porous panel 154. Particularly, in order to support a solid substrate such as the slide glass 10, a white support panel 160 or white board may be vacuum-adsorbed on the porous panel 154, Measurement of the size of the formed spots and the like can be made very easily.

In addition, the biochip layer 100 may include a humidity control unit 220 that can provide humidified air with moisture particles removed into the process chamber 104, It is possible to prevent the condensation phenomenon in the inside.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined in the following claims. It can be understood that.

10: Slide glass 20: Well plate
30, 40: sample substrate 100: biochip layer
102: base plate 104: process chamber
110: spotting unit 112: spotting pin
116: hook member 120: dispensing unit
122: Dispensing nozzle 130: Holder
134: hook groove 140:
150: vacuum chuck 152: chuck body
154: Porous panel 160: Support panel
170: Support unit 180: Cleaning unit
190: sample block 200: first measurement camera
210: second measurement camera 220: humidity control unit

Claims (15)

A spotting unit comprising at least one spotting pin for spotting a biological sample on a solid substrate;
A dispensing unit including at least one dispensing nozzle for dispensing the sample in the form of droplets on the solid substrate;
A holder to which the spotting unit and the dispensing unit are selectively mounted;
A vacuum chuck comprising a porous panel for supporting the solid substrate; And
And a driving unit for moving the holder horizontally and vertically for spotting and dispensing of the sample. 2. The apparatus of claim 1, wherein the spotting unit and the dispensing unit each include a first body and a second body on which the spotting pin and the dispensing nozzle are mounted,
And a hook member for mounting the holder on the rear surface of the first body and the second body,
Wherein a hook groove is formed in a front surface of the holder to receive the hook member. The biochip according to claim 1, wherein the vacuum chuck comprises a chuck body connected to a vacuum pump, and the porous panel is disposed on the chuck body so that vacuum pressure is applied to the porous panel from the vacuum pump. A layer. The biochip layer according to claim 3, wherein a membrane pad is disposed on the porous panel as the solid substrate. The biochip layer according to claim 3, wherein the vacuum chuck further comprises a support panel vacuum-adsorbed on the porous panel, and a slide glass as the solid substrate is disposed on the support panel. The biochip layer according to claim 3, wherein the chuck body is provided with a cooling water channel for temperature control. The biochip layer according to claim 1, further comprising a support unit for supporting at least one well plate in which the sample is accommodated. The biochip layer according to claim 1, further comprising a cleaning unit for cleaning the spotting pin and the dispensing nozzle. The vacuum cleaner according to claim 8, wherein the cleaning unit comprises: a cleaning tank to which a washer fluid for cleaning the spotting pin and the dispensing nozzle is supplied; a vacuum port for removing the sample remaining on the spotting pin; And a drain tank for discharging the sample remaining in the fencing nozzle. The biochip layer according to claim 9, wherein a channel for overflowing the washer fluid supplied to the washing tank to the drain tank is provided between the washing tank and the drain tank. The apparatus according to claim 1, further comprising: a sample tube accommodating a droplet discharged from the dispensing nozzle for a discharge test of the sample by the dispensing nozzle; and a spotting test for measuring a spot size by the spotting pin Further comprising a sample block for supporting the sample substrate. 12. The biochip layer according to claim 11, wherein a first measurement camera for measuring a spot size formed on the sample substrate is mounted on one side of the holder. The biochip layer according to claim 1, further comprising a second measurement camera for measuring a size of droplets discharged from the dispensing nozzle. The apparatus according to claim 1, further comprising: a process chamber for performing a bio chip array process using the spotting unit and the dispensing unit; and a humidity control unit for controlling humidity inside the process chamber Biochip layer. 15. The humidity control apparatus according to claim 14, wherein the humidity control unit includes a humidifier, a fan filter unit for supplying humidified air to the process chamber, and a humidity sensor for measuring humidity inside the process chamber Biochip layer to be.

Images