CN109406813A - A kind of microfluidic control device for high-throughput droplet array microfluid point sample - Google Patents
A kind of microfluidic control device for high-throughput droplet array microfluid point sample Download PDFInfo
- Publication number
- CN109406813A CN109406813A CN201811534281.3A CN201811534281A CN109406813A CN 109406813 A CN109406813 A CN 109406813A CN 201811534281 A CN201811534281 A CN 201811534281A CN 109406813 A CN109406813 A CN 109406813A
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- China
- Prior art keywords
- sample
- groove
- control device
- microfluidic control
- point sample
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- 239000007788 liquid Substances 0.000 claims description 25
- 239000002699 waste material Substances 0.000 claims description 8
- 238000010586 diagram Methods 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 210000000078 claw Anatomy 0.000 description 5
- 238000004064 recycling Methods 0.000 description 2
- 230000002000 scavenging effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
- G01N2035/1039—Micropipettes, e.g. microcapillary tubes
Abstract
The invention discloses a kind of microfluidic control devices for high-throughput droplet array microfluid point sample, including fixed plate and spotting template, fixed plate is securely removably connected at spotting template upper side, upper end is provided with sample introduction micro-pipe, array loading wells is provided in spotting template, array loading wells upper end is provided with the groove being connected to the upper end, groove is connected to sample introduction micro-pipe, the detachable structure for the microfluidic control device that the present invention uses, it is conveniently replaceable the point template of different templates, multiple array loading wells are connected to using groove, the point sample of sample can be fast implemented, greatly improve point sample efficiency, the present invention also has structure simple, it is at low cost, feature easy to operate.
Description
Technical field
The present invention relates to a kind of microfluidic control devices for high-throughput droplet array microfluid point sample, belong to microfluid point
Sample equipment technical field.
Background technique
With the research and progress of current biological medicinal career, in order to meet unicellular culture, microcell DNA fluorescence detection,
With biological cell sensing etc., people are to the array for processing miscellaneous shape in different base sample surfaces rapid, high volume
Produce increasing needs.Traditional scheme is led to by the dedicated vessel such as cell culture medium or kit
It crosses that people are manual or bulky direct current point sample instrument obtains the sample drop of bigger array.So not only it cannot be guaranteed that liquid
The flow and speed of point sample, can't control within the scope of microcell the shape of (1-100um) liquid.
Summary of the invention
The technical problem to be solved by the present invention is providing a kind of for the micro-fluidic of high-throughput droplet array microfluid point sample
Device processed, to solve above-mentioned problems of the prior art.
The technical scheme adopted by the invention is as follows: a kind of microfluidic control dress for high-throughput droplet array microfluid point sample
It sets, including fixed plate and spotting template, fixed plate is securely removably connected at spotting template upper side, and upper end is provided with sample introduction
Micro-pipe, is provided with array loading wells in spotting template, and array loading wells upper end is provided with the groove being connected to the upper end, groove with
The connection of sample introduction micro-pipe.
Preferably, above-mentioned sample introduction micro-pipe is arranged more, the groove on the upside of Duo Genjun subarray loading wells.
Preferably, above-mentioned groove is communicated with waste liquid micro-pipe.
Beneficial effects of the present invention: compared with prior art, effect of the invention is as follows:
(1) detachable structure for the microfluidic control device that the present invention uses, is conveniently replaceable the point template of different templates, using groove
Multiple array loading wells are connected to, the point sample of sample can be fast implemented, convenient for controlling the flow and speed of each point sample, realize point
The uniformity of sample, greatly improves point sample efficiency, and the present invention also has the characteristics that structure is simple, at low cost, easy to operate；
(2) more sample introduction micro-pipes of present invention setting can carry out filling liquid to multiple grooves being connected to array loading wells simultaneously, mention
High filling liquid efficiency reduces the flowing time of liquid in the trench, improves point sample efficiency；
(3) on the one hand waste liquid micro-pipe plays the role of Recycling of waste liquid, on the other hand plays scavenging action, improves the stream of spotting fluid
Dynamic property.
Detailed description of the invention
Fig. 1 is overall structure diagram；
Fig. 2 is microfluidic control device attachment structure schematic diagram；
Fig. 3 is liquid sample introduction device structural schematic diagram；
Fig. 4 is spotting template structural schematic diagram；
Fig. 5 is supporting structure schematic diagram；
Fig. 6 is all directions screw-nut driving mechanism schematic diagram of tri- axis mobile platform of XYZ.
Specific embodiment
With reference to the accompanying drawing and the present invention is described further in specific embodiment.
Embodiment 1: as shown in figs 1 to 6, a kind of microfluidic control device for high-throughput droplet array microfluid point sample,
The microfluidic control device 4 includes fixed plate 401 and spotting template 402, and fixed plate 401 is securely removably connected at spotting template
402 upper sides, upper end are provided with sample introduction micro-pipe 403, and array loading wells 404, array loading wells are provided in spotting template 402
404 upper ends are provided with the groove 405 being connected to the upper end, and groove 405 is connected to sample introduction micro-pipe 403, are provided in fixed plate 401
Screw thread guide rod, screw thread guide rod use nut check after passing through spotting template.
Preferably, above-mentioned sample introduction micro-pipe 403 is arranged more, the groove 405 of 404 upside of Duo Genjun subarray loading wells.
Preferably, above-mentioned groove 405 is communicated with waste liquid micro-pipe 406.
Embodiment 2: as shown in figs 1 to 6, a kind of high throughput droplet array microfluid spot sample device, the T word including handstand
Type frame 1, tri- axis mobile platform 2 of XYZ, point template 3 and microfluidic control device 4 are equipped with tri- axis of XYZ on the vertical plate of T font frame 1
Mobile platform 2 is equipped with point template 3 on tri- axis mobile platform 2 of XYZ, and T font frame 1 is fixedly connected with horizontal cantilever beam 5, micro-
Flow control device 4 is fixedly connected on cantilever beam 5 by bracket 6 and is located at 3 upside of point template, and microfluidic control device 4 is connected with liquid
Body sample feeding device 7.
Preferably, above-mentioned microfluidic control device 4 includes fixed plate 401 and spotting template 402, and fixed plate 401 is removably
It is fixedly connected on 402 upper side of spotting template, upper end is provided with sample introduction micro-pipe 403, array point sample is provided in spotting template 402
Hole 404,404 upper end of array loading wells are provided with the groove 405 being connected to the upper end, and groove 405 is connected to sample introduction micro-pipe 403,
Screw thread guide rod is provided in fixed plate 401, screw thread guide rod uses nut check after passing through spotting template.
Preferably, above-mentioned sample introduction micro-pipe 403 is arranged more, the groove 405 of 404 upside of Duo Genjun subarray loading wells.
Preferably, above-mentioned groove 405 is communicated with waste liquid micro-pipe 406.
Preferably, above-mentioned tri- axis mobile platform 2 of XYZ includes that sequentially connected Z axis mobile station 8, X-axis mobile station 9 and Y-axis are moved
Dynamic platform 10, Z axis mobile station 8, X-axis mobile station 9 and Y-axis mobile station 10 include slide block guide rail pair, slide plate, are connected with platform driving
The slide plate of the screw-nut pair of motor, Z axis mobile station 8 is connected on vertical plate by slide block guide rail pair, and slide plate bottom is connected with
The vertical screw-nut pair for driving it to move, the slide plate of X-axis mobile station 9 are connected to Z axis mobile station 8 by slide block guide rail pair
On the level board of slide plate connection, slide plate bottom is connected with the vertical screw-nut pair of its movement of driving, the cunning of Y-axis mobile station 10
Plate is connected on the slide plate of X-axis mobile station 9 by slide block guide rail pair, and slide plate bottom is connected with the vertical lead screw of its movement of driving
Screw pair.
Preferably, heating plate 11 is provided between above-mentioned tri- axis mobile platform 2 of XYZ and point template 3.
Preferably, it is provided with heat-conducting plate 12 between above-mentioned heating plate 11 and point template 3, is arranged between heat-conducting plate 12 and heating plate 11
There is temperature sensor 13.
Preferably, above-mentioned bracket 6 includes that I-shaped connection grillage 601, the L-type fixed jaw 602 to stand upside down and the L-type stood upside down are living
Pawl 607, I-shaped 601 lower end side of connection grillage are fixedly connected with L-type fixed jaw 602, and bottom is provided with T-type sliding slot 603, T
T-type sliding block 604 is installed, T-type sliding block 604 is fixedly connected with L-type movable claw 607, L-type fixed jaw 602 and L-type in type sliding slot 603
Movable claw 607 is connected by 605 spiral of screw rod, and the screw thread at 607 connection wire rod 605 of L-type fixed jaw 602 and L-type movable claw is anti-
To L-type fixed jaw 602 and 607 lower end inside of L-type movable claw are embedded into the groove 606 of 4 two sides of microfluidic control device, screw rod
605 one end are connected with handle 608.
Preferably, aforesaid liquid sample feeding device 7 includes flow equalizing valve 701, microcavity syringe 702 and sample injector
703, the liquid outlet of microcavity syringe 702 is connected to the inlet of flow equalizing valve 701 by pipeline, and the inlet of upside is connected with sample
Product syringe 703, flow equalizing valve 701 and microcavity syringe 702 are fixedly connected on the top of cantilever beam 5, be provided in flow equalizing valve 701 with
The fluid chamber of inlet connection, fluid chamber are provided with multiple liquid outlets, and multiple liquid outlets connect more sample introduction micro-pipes 403.
Preferably, above-mentioned microcavity syringe 702 includes microcavity 704, piston 705 and piston rod 706, and the sealing of piston 705 is set
In in microcavity 704, side is connected with piston rod 706, and piston rod 706 is pierced by microcavity 704 with being tightly connected and is connected to sample introduction drive
The output shaft of dynamic motor 707, sample introduction driving motor 707 are connected on microcavity 704 by linear bearing 708.
The advantages of above-mentioned high throughput droplet array microfluid spot sample device, is as follows:
(1) device can fast implement by microfluidic control device and three axis mobile platforms and carry out small liquid on point template
The point sample of body, point sample is quickly accurate, is adapted to different convenient for point sample at different flow profiles using different microfluidic control devices
Demand, convenient for control control flow and speed, it is time saving and energy saving to operate, which also has that structure is simple, at low cost, operation side
Just quick feature；
(2) detachable structure for the microfluidic control device that the device uses, is conveniently replaceable the point template of different templates, using groove
Multiple array loading wells are connected to, the point sample of sample can be fast implemented, greatly improve point sample efficiency；
(3) more sample introduction micro-pipes are set, filling liquid can be carried out to multiple grooves being connected to array loading wells simultaneously, improve filling liquid
Efficiency reduces the flowing time of liquid in the trench, improves point sample efficiency；
(4) on the one hand waste liquid micro-pipe plays the role of Recycling of waste liquid, on the other hand plays scavenging action, improves the stream of spotting fluid
Dynamic property；
(5) mobile by the three-dimensional of the Z axis mobile station of connection, X-axis mobile station and Y-axis mobile station, point template can be fast implemented
The adjustment of position between microfluidic control device, adjustment is quickly accurate, improves point sample efficiency；
(6) heating plate is set, is easy to implement point template constant temperature point sample, avoids the influence because of temperature change to point sample；
(7) heat-conducting plate is set, can be realized soaking transmitting, avoid because local temperature it is excessively high caused by it is uneven, setting temperature passes
Sensor is capable of the temperature of real-time monitoring heating plate, convenient for controlling the heating temperature of point template；
(8) supporting structure being arranged, downside setting clamping microfluidic control device, is conveniently replaceable the Wake control dress of different templates
It sets, realizes different liquid form point samples, using screw rod, fixed jaw and movable claw structure, be convenient for rapid-assembling/disassembling, clamping stabilization can
It leans on, is also convenient for adapting to different size of microfluidic control device, greatly improve the utilization rate of equipment, reduce equipment cost；
(9) using flow equalizing valve, the liquid sample introduction device of microcavity syringe and sample injector, it can be realized spotting fluid sample
Product being rapidly injected and quickly adding；
(10) mobile using sample introduction driving motor driving piston rod, move back and forth on linear bearing, realizes the fast of spotting fluid
Speed is added.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Within protection scope of the present invention, therefore, protection scope of the present invention should be based on the protection scope of the described claims lid.
Claims (3)
1. a kind of microfluidic control device for high-throughput droplet array microfluid point sample, it is characterised in that: including fixed plate
(401) and spotting template (402), fixed plate (401) are securely removably connected at spotting template (402) upper side, and upper end is set
It is equipped with sample introduction micro-pipe (403), is provided in spotting template (402) array loading wells (404), array loading wells (404) upper end is set
It is equipped with the groove (405) being connected to the upper end, groove (405) is connected to sample introduction micro-pipe (403).
2. a kind of microfluidic control device for high-throughput droplet array microfluid point sample according to claim 1, special
Sign is: sample introduction micro-pipe (403) is arranged more, the groove (405) on the upside of Duo Genjun subarray loading wells (404).
3. a kind of microfluidic control device for high-throughput droplet array microfluid point sample according to claim 1, special
Sign is: groove (405) is communicated with waste liquid micro-pipe (406).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811534281.3A CN109406813A (en) | 2018-12-14 | 2018-12-14 | A kind of microfluidic control device for high-throughput droplet array microfluid point sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811534281.3A CN109406813A (en) | 2018-12-14 | 2018-12-14 | A kind of microfluidic control device for high-throughput droplet array microfluid point sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109406813A true CN109406813A (en) | 2019-03-01 |
Family
ID=65459265
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811534281.3A Pending CN109406813A (en) | 2018-12-14 | 2018-12-14 | A kind of microfluidic control device for high-throughput droplet array microfluid point sample |
Country Status (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113996356A (en) * | 2021-10-27 | 2022-02-01 | 北京京东方技术开发有限公司 | Micro-fluidic chip, manufacturing method thereof and medical detection equipment |
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