CN107794222A - The cell culture apparatus of dynamic microenvironment in analogue body - Google Patents
The cell culture apparatus of dynamic microenvironment in analogue body Download PDFInfo
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- CN107794222A CN107794222A CN201711007010.8A CN201711007010A CN107794222A CN 107794222 A CN107794222 A CN 107794222A CN 201711007010 A CN201711007010 A CN 201711007010A CN 107794222 A CN107794222 A CN 107794222A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12M45/00—Means for pre-treatment of biological substances
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Abstract
The invention discloses the cell culture apparatus of dynamic microenvironment in analogue body, including housing and the plane cover located at enclosure interior, culture dish placement rack, stretching device, platform base, cooling mechanism, in addition to top cover, real time viewing system, electric stimulation, control system, CO2Conveying device, changing liquid automatically device and clamping device, stretching device includes the transmission mechanism and pulling force sensor of control clamping device movement, the setting of multiple movable clamps, during cell culture, it can be achieved to carry out biomembrane unidirectional, two-way, multidirectional cyclic tension, and the stretching time, stretching frequency and stretch range of biomembrane are controlled, electro photoluminescence is carried out to cell simultaneously, the joint that electric pulling force stimulates can be realized, the microenvironment residing for internal cell is more realistically simulated, the research for cell, the growth in vitro of tissue provides condition.
Description
Technical field
The present invention relates to technical field of cell culture, the cell culture more particularly, to dynamic microenvironment in analogue body fills
Put.
Background technology
In organizational project, the external functionalization culture of cell, tissue has turned into the core technology of an organizational project,
It is the essential technical foundation to form organizational project industry, but Cell culture invitro still has several drawbacks at present.
In terms of mechanics, the experimental method of analogue body interior mechanics environment mainly has Osima jacoti, Osima excavata method, substrate tension method, quiet
Hydrostatic(al) process, hoop stress method；The experimental method of the outer mechanical environment of analogue body mainly has microgravity cell culture method, centrifugal force field
Method, gas pressurized method, sound stimulation method, microbeam irradiation method.Substrate tension technology starts from the appearance of elastic cell's culture membrane.
The mentality of designing of substrate tension technology be using biomembrane as base material, by by cell seeding on biomembrane, by device
Produce controllable motion and make it that biomembrane deforms upon, and cell of the culture on biofilm surface because of the deformation of substrate and by
Power, so as to realize the possibility of cell growth law study under tensile stress, this loading technique is current a kind of ideal
Experimental technique, available for the external Mechanical loading of various cells, be mainly used in studying distraction force and cultured cell in vitro bred
With the influence of metabolism etc., its loading technique mainly has rectangular base stretching, circular-base stretching etc., the wherein stretching of circular-base
It is more common.
In terms of electricity, electricity environment is one of important microenvironment of internal cell growth.In feelings existing for physiology electric field
Under condition, cell produces division, differentiation and migration.The cell culture environment for not having electric field has lacked a kind of embryo's nature growth course
In possessed extracellular environment.Can be cell in vitro group by the electricity microenvironment residing for analogue body inner cell, tissue growth
The growth knitted provides more real environment, can make that cell is quick, high-quality propagation, as electro photoluminescence can induce human osteoblast cell's calcium
The increase of ion concentration, promote the secretion of propagation, growth factor and the synthesis of extracellular matrix of Gegenbaur's cell.
In existing Cell culture invitro technology, it is common that culture dish is positioned in incubator, after culture a period of time again
Culture dish is taken out, stimulated with carrying out mechanics or electricity to it, this method efficiency is low, and training quality is not high.
The content of the invention
In order to solve above-mentioned prior art problem, the invention provides the cell culture dress of dynamic microenvironment in analogue body
Put, including housing, and be used in housing the clamping device for clamping biological structure, the clamping device includes at least two
Movable clamp, the biological structure is between two fixtures；The stretching device of clamping device movement is controlled in housing；Institute
State the culture dish placement rack being provided between clamping device for culture dish holding；And for detecting the pulling force of biological structure pulling force
Sensor, and by pulling force signal output.
In order to further realize the present invention, pulling force sensor is provided with the fixture.
In order to further realize the present invention, the fixture includes the tune of upper grip, lower chuck and regulation upper grip movement
Component is saved, upper grip includes upper bare terminal end, upper connecting portion, and the bottom of upper connecting portion is connected with upper bare terminal end, and upper connecting portion is in inverted L
Shape, lower chuck are arranged on the lower section of upper grip, and lower chuck includes lower bare terminal end, lower connecting portion, upper bare terminal end and lower bare terminal end
Clamping part is formed, for biomembrane to be clamped, lower connecting portion is set in parallel in the lower section of connecting portion, and the shape of lower connecting portion is
With the shape identical inverted L shape of upper connecting portion, lower connecting portion is provided with chute, and upper connecting portion glides on the chute along lower connecting portion
It is dynamic, so as to drive upper bare terminal end close or use adjusting screw rod and nut away from lower bare terminal end, the adjusting part, by twisting
Dynamic adjusting screw rod is so as to driving upper bare terminal end close to or away from lower bare terminal end.
In order to further realize the present invention, the adjusting part is adjusting screw rod, the horizontal stroke of the upper connecting portion of the upper grip
The screw of up/down perforation is provided with to section, the lower chuck correspondence position is provided with the second through hole, and the bottom of adjusting screw rod is arranged on the
In two through holes, the top of adjusting screw rod passes through the screw of upper connecting portion, and adjusting screw rod is threadedly coupled with upper connecting portion, by turning
Adjusting screw rod drives upper grip to move up and down, so as to drive the upper bare terminal end of upper grip close to away from lower bare terminal end, realizing folder
Tightly with unclamping biological structure.
In order to further realize the present invention, the lower connecting portion of the lower chuck sets to the concave to form cunning along its length
Groove, the upper connecting portion of the upper grip slide up and down along chute.
In order to further realize the present invention, in addition to limiting section, the position that the lower chuck is located at lower connecting portion is grown along it
Degree direction is provided with one section of slide opening, is provided with through hole corresponding to the upper grip part of slide opening position, the limiting section sequentially passes through through hole
And upper connecting portion is limited in the slide opening of lower connecting portion and slided by slide opening, the limiting section, connecting portion is avoided to depart from chute, together
When the distance of sliding upper connecting portion be spacing slide opening length.
In order to further realize the present invention, in addition to electric stimulation, the electric stimulation includes positive and negative electrode.
In order to further realize the present invention, in addition to top cover, the top cover inwall is fixedly connected with elastic component, the elastic component
Bottom be fixedly connected with positive and negative electrode, the positive and negative electrode is longitudinally disposed.
In order to further realize the present invention, the stretching device includes transmission mechanism, transmission device include two fixed seats,
Stepper motor, screw mandrel and stroke lever, the stepper motor are fixedly installed in the fixed seat provided with through hole, and the screw mandrel is arranged on
Between two fixed seats, described screw mandrel one end is fixedly connected with the output shaft of motor, and through fixed seat through hole, the other end with
The screw connection of another fixed seat, stroke lever one end are fixedly connected with lower chuck, and the stroke lever other end is provided with screw, the screw
It is connected with wire rod thread, the stepper motor drives screw mandrel to rotate, and realizes stroke lever horizontal rectilinear motion, and then realizes stretching folder
Tool movement.
In order to further realize the present invention, in addition to for the cell culture apparatus provide CO2CO2Conveying device, the cell
Culture dish in culture apparatus provide the changing liquid automatically device for changing liquid function and restrained stretching device stretching time, stretching frequency with
And the control system of stretch range.
Beneficial effect：
1st, the present invention in enclosure interior by being provided with plane cover, and the center of plane cover, which is provided with, places bore, along placement
Multiple dragging tracks are provided with around bore, each dragging track is provided with a fixture, and fixture clamps to biomembrane, fixture
Moved by stepper motor on dragging track, stepper motor, the level of step motor control fixture are controlled by control system
Stress loading, and direction of the fixture of the present invention along culture dish placement rack to ambient radiation is set, and fixture may be provided in many ways
To, it is achievable that unidirectional, two-way, multidirectional cyclic tension is carried out to biomembrane, and can control the stretching time to biomembrane, stretching
Frequency and stretch range, mechanical stimulation can be so loaded while cell culture, so that cell culture quality is higher, training
Support improved efficiency；
2nd, the present invention is provided with electric stimulation, it is possible to achieve to cell interval or lasting electro photoluminescence, passes through analogue body
Electricity microenvironment residing for inner cell, tissue growth, and be combined with mechanical stimulation, can be that the growth of cell in vitro, tissue carries
For closer to like internal environment, so as to complete the functionalization culture of tissue.The electric stimulation of the present invention includes positive and negative electrode, bullet
Spring, spring play cushioning effect, and this set can avoid electrode from being contacted firmly with biomembrane, ensure being smoothed out for experiment.
3rd, electric stimulation of the invention is provided with lowering or hoisting gear, and lowering or hoisting gear makes electrode scalable, need not carry out
, can be by positive and negative electrode toward moving up, so as to avoid electrode from being placed in for a long time in nutrient solution when electro photoluminescence is tested, influence electrode uses the longevity
Life, and electrode liftable, it can be achieved to the batch (-type) stimulation of cell or long stimulus, flexibility is stronger, the lowering or hoisting gear
Setting allow the present apparatus have independent mechanical stimulation, mechanics-electricity at the same stimulate, individually electrically stimulate etc. various ways, so as to
Enable cell more accurately true environment in analogue body.
4th, the present invention is provided with strain detector, and strain detector is installed on biomembrane, for realizing accurate reality
When strain monitoring, strain detector uses foil gauge or strain transducer, can real―time precision measurment film strain；Traditional is logical
The method for crossing the strain that loading is calculated using displacement and sample length, inexactness be present, and present invention measurement biomembrane should
Change accurately and reliably, rapidly.
5th, fixture of the invention is provided with the pulling force sensor for detecting biological membrane stress, and it is controllable to realize pulling force, and can
To control influence of the different stress intensities to cell；It is traditional by applying positive air pressure to the bottom of biomembrane or negative pressure carrys out reality
In the method for the deformation of existing biomembrane, the mechanical stimulation that cell is subject to is uneven, and the size of power is uncontrollable, experiment be present
Quantitative uncertainty, experimental design is caused certain error or deficiency to be present.And stretching clamp of the present invention is provided with pulling force
Inductor, stretching clamp is arranged on the radiation direction along culture dish placement rack, and stretching clamp may be provided at multiple directions, pulling force
Inductor can measure the stress intensity of respective direction, then according to the growth conditions of cell, adjust the drawing of respective direction in time
Power, the pulling force that multiple directions can be achieved either synchronously or asynchronously adjust, i.e., can realize different stress, the stimulation of different directions；Pulling force
Inductor can be measured accordingly to the stress of biomembrane, avoided and tested quantitative uncertainty, show that experimental result is more accurate.
6th, grip device of the invention uses multiple movable clamps, and fixture includes pressing from both sides in upper grip, lower chuck and regulation
The mobile adjusting part of head, upper grip include upper bare terminal end, upper connecting portion, and the bottom of upper connecting portion is connected with upper bare terminal end, on
Connecting portion is in inverted l shape, and lower chuck is arranged on the lower section of upper grip, and lower chuck includes lower bare terminal end, lower connecting portion, upper bare terminal end
Clamping part is formed with lower bare terminal end, for biomembrane to be clamped, lower connecting portion is set in parallel in the lower section of connecting portion, lower connection
The shape in portion is to be provided with chute with upper connecting portion shape identical inverted L shape, lower connecting portion, and upper connecting portion is along lower connecting portion chute
Slide up and down, adjusting part uses adjusting screw rod and nut；By turning adjusting screw rod so as to drive upper bare terminal end close with it is remote
From lower bare terminal end, to realize the clamping of biomembrane and release, this is simple in construction, convenient, quickly.
7th, whole fixture of the invention is oblique, and relative to traditional vertical stratification that uses, on the one hand this set is kept away
Tissue Culture Dish side wall has been opened, it is more convenient in culture dish holding, fixture will not be caused to touch culture dish, be advantageous to cell
Smoothly culture；On the other hand being obliquely installed can facilitate biomembrane to put into and take out, so need not be by other instruments, it is possible to
The simple nut for turning screw rod drives upper grip to slide up and down, and easily and quickly realizes the clamping or release of film.
8th, the present invention is close to lower bare terminal end on chute by being provided with one section of spacing slide opening, and spacing slide opening is along sloping portion
Length direction is set, and the present invention corresponds at spacing slide opening in the upper connecting portion of upper grip is provided with through hole, and limiting section sequentially passes through
Upper connecting portion is limited in the chute of lower connecting portion and slided by through hole and slide opening, limiting section, avoids connecting portion from departing from chute, and
Upper grip can be avoided to rock, it is impossible to biomembrane is accurately clamped, while the distance that upper connecting portion is slided is the length of spacing slide opening,
When limiting section slides into the bottom of spacing hole, upper bare terminal end is fitted tightly on lower bare terminal end.
9th, the present invention is additionally provided with top cover, and the growth for being arranged to cell of top cover provides pollution-free, anti-culture medium evaporation, wet
The environment of degree and proper temperature.Tissue Culture Dish can be with direct experiment and culture in the incubator of the present invention, it is not necessary to Gai Pei
Ware lid is supported, this setting for being combined culture dish with incubator, experimental procedure is simplified, saves the time, and is avoided because of frequency
It is numerous to take out Tissue Culture Dish and cause to influence caused by pollution or cell growth state.
10th, culture dish placement rack of the invention by rotation height regulation ring, can realize the height of culture dish on plummer
Control, costly, works as culture dish because when doing tension test, fixture should try one's best close to culture dish bottom, general nutrient solution
When position is too low, typically fixture bare terminal end is immersed in nutrient solution by adding nutrient solution, biomembrane is totally submerged
In nutrient solution；The present invention can avoid adding nutrient solution by adjusting culture dish rising, so as to avoid the waste of nutrient solution.
11st, the present invention is additionally provided with real time viewing system, and the image of Tissue Culture Dish is sent into cell culture by optical fiber
Outside device, and the form of cell can be clearly observable by eyepiece, in the other end of optical fiber, with CCD camera reception optical fiber
The image transmitted, after the DSP Processor through CCD camera is acquired and stored to figure, by the second radio receiving transmitting module
It is transmitted on the first radio receiving transmitting module, is resent on control system computer, in case at real-time monitored, storage or image
Reason, computer go back the authenticity of original image by image procossing, and the cultivation conditions of cell are analyzed and to some important
Stage is prompted, and staff is observed on special time, is analyzed the state of cell.
12nd, the present invention is provided with cooling mechanism in cell culture placement rack lower end, and cooling mechanism includes radiator and radiation air
Fan, radiator can collect heat caused by incubator work, radiator fan dissipating through housing by the heat that radiator is collected
Outside hot hole discharge housing, the temperature of the nutrient solution in culture dish is set to be within the scope of the thermostat temperature specifically set.
Brief description of the drawings
Fig. 1 is the exploded perspective view of the present invention；
Fig. 2 is the structural representation of stretching device of the present invention；
Fig. 3 is the structural representation of clamping device of the present invention；
Fig. 4 is the structural representation of upper grip of the present invention；
Fig. 5 is the structural representation of lower chuck of the present invention；
Fig. 6 is the structural representation of limiting section of the present invention；
Fig. 7 is that the biomembrane of the present invention is provided with the structural representation of strain-Sensing device；
Fig. 8 is the control principle drawing of real time viewing system；
Fig. 9 is the structural representation that the top cover of embodiment 2 is provided with electric stimulation；
Figure 10 is the structural representation of the electric stimulation of embodiment 3；
Figure 11 is the structural representation that the present invention does not install clamping device and top cover；
Figure 12 is the structural representation after Figure 11 installs fixtures；
Figure 13 is the overall structure diagram after top cover；
Figure 14 is the CO of the present invention2The structural representation of conveying device；
Figure 15 is the changing liquid automatically schematic device of the present invention；
Figure 16 is the structural representation of the changing liquid automatically device discrepancy joint of the present invention；
Figure 17 is the sectional view of the changing liquid automatically device discrepancy joint of Figure 16 present invention；
Figure 18 is the top view of the changing liquid automatically device discrepancy joint of Figure 16 present invention.
Embodiment
The present invention is further elaborated with reference to the accompanying drawings and examples, and these accompanying drawings are simplified schematic diagram, only
Illustrate the basic structure of the present invention in a schematic way, the direction of this specific implementation is using Fig. 1 directions as standard.
As shown in Fig. 1~18, the invention discloses a kind of cell culture apparatus of dynamic microenvironment in analogue body, it includes
Housing 1, plane cover 2, culture dish placement rack 3, clamping device 41, stretching device 4, strain detector 5, platform base 6, radiating
Mechanism 7, top cover 8, real time viewing system 9, electric stimulation 10, control system 20, CO2Conveying device 30, changing liquid automatically device
40 and display screen (not shown), wherein：
Embodiment 1
The opening-like structure in the upper end of housing 1, in square or cuboid, this shape is certainly not limited to, the one of housing 1 is lateral
On convex to form a sidepiece 11, housing 1 is internally formed cavity, culture dish placement rack 3, stretching device 4, platform base 6, radiating machine
Structure 7 is arranged in cavity.
Plane cover 2 is arranged at the upper opening of housing 1, and plane cover 2 is adapted with housing 1, plane cover 2 and shell
Body 1, which can be screwed or block, sets connection, and the upper surface of plane cover 2 is provided centrally with placing bore 21, placement bore
21 are set in up/down perforation, place bore 21 as circle, are outwards spaced along the circumferential edge for placing bore 21 on plane cover 2
Provided with several sliding shape tracks 22, sliding shape track 22 is in strip, and the sliding up/down perforation plane cover 2 of shape track 22.
Culture dish placement rack 3 is used for the height for adjusting culture dish, and culture dish placement rack 3 includes height regulation ring 31, carrying
Platform 32, height adjustment seat 33.Height regulation ring 31, which is arranged on, places at bore 21, and height regulation ring 31 is in annular, and height is adjusted
The section outer wall of ring 31 is provided with ring shaped thread；And the upper surface of height regulation ring 31 is provided with two breach 311, consequently facilitating rotation height
Regulation ring 31, plummer 32 are fixedly installed on the bottom of height regulation ring 31, or are embedded inside regulation ring 31, so as to shape
Into a loading floor, plummer 32 is rounded, and the plummer 32 is used for culture dish holding 502, and height adjustment seat 33 is in hollow
It is cylindric, the inwall of height adjustment seat 33 is provided with ring shaped thread, and the internal thread of height adjustment seat 33 and 31 outer spiral shell of height regulation ring
Line coordinates, the height of adjustable carrier platform 32 during such rotation height regulation ring 32, bottom and the platform base of height adjustment seat 33
Seat 6 is fixedly connected；
The culture dish placement rack 3 of the present invention by rotation height regulation ring 32, can realize the height control of plummer 32, enter
And the height control of cell training culture dish 502 is realized, because when doing tension test, fixture trains culture dish close to cell as far as possible
502 bottoms, general nutrient solution costly, when culture dish position is too low, are typically pressed from both sides by adding nutrient solution come fixture
Hold end to be immersed in nutrient solution so that biomembrane 503 can be completely submerged in nutrient solution, and the present invention can be by adjusting cell
Training culture dish 502 is up adjusted to avoid adding nutrient solution, so as to avoid the waste of nutrient solution.
Clamping device 41 is used to clamp biomembrane 503, and clamping device 41 is at least two movable clamps, and each slides rail
Road is correspondingly provided with a fixture 41, and the present invention sets at least to two, and the present invention is exemplified by four, and each two fixture is in same
One straight line is set, and fixture 41 includes upper grip 411, lower chuck 412, adjusting part 413, wherein, upper grip 411 includes upper clamping
End 4111, upper connecting portion 4112, upper bare terminal end 4111 is a slab construction, and upper bare terminal end 4111 is arranged with resilient sleeve (in figure not
Display), avoid damaging biomembrane 503 because of biomembrane 503 and the upper hard contact of bare terminal end 4111, the bottom of upper connecting portion 4112
With the upper integrally connected of bare terminal end 4111 or being fixedly connected, and connect at an angle, preferably obtuse angle, upper connecting portion 4112 is falls
L shape, wherein, the longitudinal component of upper connecting portion 4112 is oblique so that whole upper grip 411 is inclined to set, tilting section
Through hole 41121 is provided with, upper connecting portion 4112 is located at the screw 41122 of the lateral part provided with up/down perforation on top；
Lower chuck 412 is arranged on the lower section of upper grip 411, and lower chuck 412 includes lower bare terminal end 4121, lower connecting portion
4122, and lower bare terminal end 4121 be arranged in parallel with upper bare terminal end 4111, upper bare terminal end 4111 is formed with lower bare terminal end 4121 and clamped
Portion, for substrate material to be clamped, lower bare terminal end 4112 is arranged with resilient sleeve (not shown), avoid because biomembrane 503 with
The lower hard contact of bare terminal end 4121 and damage biomembrane 503；Lower connecting portion 4122 is set in parallel in the lower section of connecting portion 4112,
Lower connecting portion 4122 is inside with the upper shape identical inverted l shape of connecting portion 4112, the whole sloping portion of lower connecting portion 4122
It is recessed to be slided up and down into chute 41221, upper connecting portion 4112 in chute 41221, so as to drive upper bare terminal end 4111 close with
Away from lower bare terminal end 4121, and then clamping and loose unloading biomembrane 503 are realized, be provided with chute 41221 close to lower bare terminal end 4121
One section of spacing slide opening 41222, spacing slide opening 41222 are set along sloping portion length direction, and the present invention is set in through hole 41121
Upper connecting portion 4112 is limited in the chute of lower connecting portion 4122 and slided by limited location portion 4113, limiting section 4113, the company of avoiding
Socket part 4112 departs from chute, and upper grip 411 can be avoided to rock, it is impossible to and it is accurate to clamp biomembrane 503, while by upper connecting portion
4112 distances slided are the length of spacing slide opening 41222, when limiting section 4113 slides into the bottom of spacing slide opening 41222,
Upper bare terminal end 4111 is fitted tightly on lower bare terminal end 4121；Limiting section 4113 can use banking pin of the upper and lower ends with cap,
The head of a nail through the bottom of first through hole 41121 of banking pin is slidably installed in the both ends of spacing slide opening 41222, as shown in fig. 6, can also adopt
Realized with following structure, including upper and lower two fixed blocks 41131, and it is connected to the connection bar portion of two fixed blocks 41131
41132, the width of upper fixed block 41131 is more than first through hole 41121, and the width of lower fixed block 41131 is more than spacing slide opening
41222 width, connection bar portion 41132 pass through first through hole 41121, upper and lower two fixed blocks 41131 be brought into close contact respectively in
Upper connecting portion 4112 and lower connecting portion 4122, the slip of upper connecting portion 4112 drive lower fixed block 41131 along spacing slide opening
41222 slide；The lateral part of lower connecting portion 4122 is provided with the second through hole 41223 of up/down perforation, and adjusting part 413 includes adjusting
Screw rod 4131, nut 4132 are saved, the bottom of adjusting screw rod 4131 is arranged in the second through hole 41223, the top of adjusting screw rod 4131
The screw through upper connecting portion 4112 is held, and is fixedly connected with nut 4132, adjusting screw rod 4131 and the upper screw thread of connecting portion 4112
Connect, in preferred embodiment, the length of adjusting screw rod 4131 is identical with the length of spacing slide opening 41222, turns nut
4132, nut 4132 drives adjusting screw rod 4131 to rotate, so that upper grip 411 moves up and down, and then adjusts upper bare terminal end
4111 with the distance of lower bare terminal end 4121, further realize and clamp and the function of loose unloading elastic biological film 503.
The stretchable cell biological film 503 of stretching device 4, stretching device 4 include the driver that control clamping device 41 moves
Structure 42 and pulling force sensor 43, wherein:
Transmission mechanism 42 includes two fixed seats 421, stepper motor 422, screw mandrel 423 and stroke lever 424, two fixed seats
421 it is parallel longitudinal be fixedly installed on platform base 6, one of fixed seat 421 is provided with through hole 4211, and the size of through hole 4211 is big
In the diameter of screw mandrel, another fixed seat corresponds to lead to the hole site and is provided with screw 4212；Stepper motor 422 is fixedly installed on provided with logical
In the fixed seat 421 in hole；Screw mandrel 423 is arranged between two fixed seats 421, the one end of screw mandrel 423 and the output of stepper motor 422
Axle is fixedly connected, and is connected through the through hole of fixed seat 421, the other end with the screw of another fixed seat 421；Stroke lever 424
One end is fixedly connected with lower chuck 412, can be fixed by screw, and the other end of stroke lever 424 is provided with screw, the screw and screw mandrel
423 threaded connections, stepper motor 422 drive screw mandrel 423 to rotate, and realize the horizontal rectilinear motion of stroke lever 424, and then realize and draw
Stretch dragging track 22 of the fixture 41 along plane cover 2 to move, lower chuck can be driven to do level to the cell on biomembrane 503
Linear motion, to carry out loading stretching to the cell of attachment, simulates internal cell, tissue stretching force environment, is cell, group
The research for the growth in vitro knitted provides condition.
The precision often walked can be controlled in 3%-5% from stepper motor 422, and the mistake of a step will not be heaped and arrived
In next step, thus have preferable bearing accuracy and motion repeatability.
Pulling force inductor 43 is set on the holder 41, may be provided on upper grip 411 or lower chuck 412, pulling force sensor
43 are able to detect that pulling force suffered by biomembrane 503, and send information to control system 20, so as to control stepper motor 422,
And then stepper motor 422 controls the horizontal stress loading of fixture 41, it is controllable to realize pulling force, and different stress can be controlled big
The small influence to cell；Present clip 41 is arranged on the setting of the ambient radiation direction of culture dish placement rack 3, and fixture 41 is settable
In multiple directions, this set can unidirectional, two-way, multidirectionally control and cyclic tension is carried out to biomembrane 503, and controllable to life
Stretching time, stretching frequency and the stretch range of thing film 503.
If do not add pulling force inductor 43, such as by applying positive air pressure or negative pressure to the bottom of biomembrane 503 come real
The method of the deformation of existing biomembrane 503, it is uneven that the power that cell is subject to, which stimulates, and the size of power is uncontrollable, experiment be present
Quantitative uncertainty, experimental design is caused certain error or deficiency to be present.And stretching clamp 41 of the present invention is provided with drawing
Power inductor 43, stretching clamp 41 may be provided at multiple directions, and pulling force inductor 43 can measure the stress intensity of respective direction,
Then according to the growth conditions of cell, the pulling force of respective direction is adjusted in time, the pulling force of multiple directions can be achieved either synchronously or asynchronously
Adjustment, i.e., can realize different stress, the stimulation of different directions；Pulling force inductor 43 can be measured accordingly to biomembrane 503
Stress, avoids and tests quantitative uncertainty, and the experimental result drawn is more accurate.
Strain detector 5 is used to realize accurate real-time strain monitoring, and strain detector 5 is passed using foil gauge or strain
Sensor, as shown in fig. 7, foil gauge is arranged on biomembrane 503, biomembrane 503 of the invention uses elastic biological film, will strain
Piece is labelled to the strain of the energy real―time precision measurment of biomembrane 503 biomembrane 503, traditional to be calculated by using displacement and sample length
Go out the method for the strain of loading, inexactness be present, and present invention measurement biomembrane 503 should change accurately and reliably, rapidly.
Platform base 6 is arranged on the lower section of stretching device 4, and the center of platform base 6 corresponds to culture dish placement rack 3
Position be provided with third through-hole 61, and its periphery is provided with several through holes, and heat can pass from through hole.
Cooling mechanism 7 is arranged on the lower section of platform base 6, and cooling mechanism 7 includes radiator 71 and radiator fan 72, radiating
The upper end of device 71 is fixedly connected with platform base 6, and the outer end of radiator 71 is connected with one end of radiator fan 72, and radiator 71 can
To collect heat caused by incubator work, radiator fan 72 arranges heat emission hole of the heat that radiator 71 is collected through housing 1
Go out outside housing 1, the temperature of the nutrient solution in culture dish is within the scope of the thermostat temperature specifically set.
The shapes and sizes of top cover 8 are adapted with housing 1, and the lower end of top cover 8 is set in opening, the surrounding and shell of top cover 8
The upper end sealed set of body 1, top cover 8 set (card is set) on housing 1, the growth for being arranged to cell of top cover 8 provide it is pollution-free,
The environment of anti-nutrient solution evaporation, humidity and proper temperature.Tissue Culture Dish 502 can be with direct experiment in the incubator of the present invention
And culture, it is not necessary to lid culture dish lid, this setting for being combined culture dish with incubator, simplify experimental procedure, save
Time, and avoid causing because frequently taking out Tissue Culture Dish 502 influence caused by contamination of cells or cell growth state.
Real time viewing system 9 includes CCD camera 91, optical fiber 92, the first data transmit-receive module 93, the second data transmit-receive mould
Block 94, optical fiber 92 are connected with CCD camera 91, and CCD camera 91 is arranged on top cover 8 or on the sidepiece 11 of housing 1, and light
The top that fibre 92 is suspended from culture dish, CCD camera 91 are connected with the second data transmit-receive module 94, the first data transmit-receive module 93 and
Second data transmit-receive module 94 is by being wirelessly connected, and the first data transmit-receive module 93 is connected with control system 20, CCD camera 91
Cell image is delivered to the first data transmit-receive module 93 by optical fiber 92, the first data transmit-receive module 93 sends data message
To the second data transmit-receive module 94 and then display is stored by control system 20.
The image of Tissue Culture Dish 502 is sent to outside cell culture apparatus by optical fiber 92, and can be clear by eyepiece
It was observed that the form of cell, the image that the reception optical fiber 92 of CCD camera 91 transmits, the DSP Processor through CCD camera 91 is to figure
After shape is acquired and stored, it is transmitted on the first data transmit-receive module 93, is resent to by the second data transmit-receive module 94
On control system computer, in case real-time monitored, storage or image procossing, computer goes back the true of original image by image procossing
Reality, the cultivation conditions of cell are analyzed, and some important stages are prompted so that staff can be specific
The state of cell is observed, analyzed on time.
The present invention can not only carry out mechanical stimulation to cell, while can also carry out electricity stimulation, realize multi-functional
Property.
Embodiment 2
The present embodiment be on the basis of embodiment 1, it is further to the cell culture apparatus to be supplemented, by following
Mode realizes that electric stimulation 10 includes positive electrode 101, negative electrode 102, signal generator (not marked in figure), elastic component
103；Wherein, positive electrode 101 and negative electrode 102 are arranged on top cover 8, arranged in parallel, positive electrode 101 and negative electrode 102
Between at intervals of 5-15mm, when top cover 8 covers, positive electrode 101 can be contradicted on biomembrane 503 with negative electrode 102；Just
Negative electrode connects signal generator by electrode wires, and positive and negative electrode, electrode wires and signal generator form electro photoluminescence loading system,
By the voltage or electric current that change signal generator, there is provided different size of electro photoluminescence, electro photoluminescence are constant voltage, pulse voltage
Or DC micro-electric stream, produced by signal generator.
When the present apparatus carries out cell culture, the through hole of positive and negative electrode line through top cover 8 is connected with signal generator.Top cover 8
Duct and electrode wires junction be sealing.
The bottom of two elastic components 103 is fixedly connected with the inside of top cover 8, bottom and the both positive and negative polarity electrode of elastic component 103
It is fixedly connected, elastic component 103 can use spring, and the setting of elastic component 103 can avoid electrode and the hard contact of biomembrane 503, from
And cushioning effect is played, ensure being smoothed out for experiment.If being not provided with spring 103 in electrode upper end, when by top cover 8 cover to
When at housing 1, in order that electro photoluminescence is more accurate, typically electrode can be abutted to biomembrane 503, and electrode typically has hardness,
Electrode is directly contacted firmly with biomembrane 503, and certain loss is on the one hand had to biomembrane 503, and on the other hand, electrode can extrude
To cell, the bigger possible process for causing clasmatosis death, influenceing experiment；The present invention is by positive electrode 101, negative electrode 102
Upper end is connected with spring 103, because spring plays cushioning effect, electrode can be avoided to be contacted firmly with biomembrane 503, further ensured
Experiment is smoothed out.
Embodiment 3
The present embodiment be on the basis of embodiment 2, it is further to electric stimulation to be improved, pass through following mode
Realize, electric stimulation 10 includes positive electrode 101, negative electrode 102, signal generator (not shown), elastic component 103, liter
Falling unit 104；Lowering or hoisting gear 104 includes motor 1041, screw mandrel 1042, electrode tip holder 1043, stop collar 1044, and motor 1041 is fixed
It is arranged on the inwall of top cover 8, the output shaft of motor 1041 is longitudinally disposed, and the output shaft of motor 1041 is fixedly connected with screw mandrel 1042, silk
Bar 1042 is in longitudinally disposed, and the center of electrode tip holder 1043 corresponds to the screw that the position of screw mandrel 1042 is provided with up/down perforation, screw mandrel
1042 pass through the screw of electrode tip holder 1043 and stretch out outside screw, and the left and right ends bottom of electrode tip holder 1043 is fixedly connected with elastic component 103,
The bottom of elastic component 103 is fixedly connected with positive electrode 101 and negative electrode 102, and the bottom of screw mandrel 1042 is arranged with stop collar 1044, silk
Convert rotational motion is linear motion in the presence of motor 1041 by bar 1042, so as to drive the rise and fall of stop collar 1044,
And then driving positive and negative electrode to realize lifting, the effect of stop collar 1044 prevents electrode tip holder 1043 from departing from screw mandrel 1042.
The setting of lowering or hoisting gear 104 in the present embodiment, can be past by positive and negative electrode when that need not carry out electro photoluminescence experiment
Move up, so as to avoid electrode from being placed in for a long time in nutrient solution, influence the service life of electrode, and electrode liftable, can be to cell
Batch (-type) stimulation or long stimulus are realized, flexibility is stronger, allows the present apparatus to have independent mechanical stimulation, mechanics-electricity same
When stimulate, individually electrically stimulate etc. various ways so that the environment that cell can more accurately in analog approximation body, elastic component
103 effect is same as Example 2, is not described herein.
Embodiment 4
The present embodiment be on the basis of embodiment 3, it is further to the cell culture apparatus to be supplemented, by following
Mode realizes that control system 20, which is adopted, computerizeds control, and control system 20 is connected with stretching device 4, pulling force sensor 43, display
Screen is arranged in the side wall of housing 1, and display screen is connected with control system 20.
Control system 20 controls transmission mechanism 42 and pulling force inductor 43 to determine stretching frequency, stretch range, stretching
Time；Stretching frequency, stretch range, stretching time are set on a display screen, tests, can obtain by the stretching of certain time
The relation of pulling force, stretching frequency, stretch range and stretching time and cell growth state；
Stretching device 4, control system 20 be combined to cell culture experiments are simpler, side applied to making in cell culture
Just, save the time and experiment is more accurate.
When doing cell experiment, such as the irritant test of cell, if inquiring into different incentive condition cell growth shapes
The influence of state, workload is bigger during experimental implementation, biological because strictly speaking experiment includes that biology repeats and experiment repeats
Learn and repeat to be exactly one group of experiment same batch cell, generally require 3 wares, experiment repeats to refer to identical experiment different batches, i.e., not
Same experiment number, general 3 times (different batches cell), if inquiring into incentive condition has 3 levels (identical frequency of stimulation, thorn
The sharp time divides 10min, 30min and 1h) so identical frequency of stimulation, the experiment of different stimulated time will need 9 wares, 93, wares batch
Secondary cytositimulation difference 10min, 30min and 1h take out observation cell growth state, and workload is bigger during experimental implementation, and sees
Examine time meeting difference, take out the more growth conditions that can also influence cell of number, these may all cause experimental error larger.
And the control system 20 in the culture apparatus of the present invention is by controlling transmission mechanism 42 and pulling force inductor 43 can be with
Determine stretching frequency, stretch range, stretching time；By certain time stretching test, can obtain pulling force, stretching frequency,
The relation of stretch range and stretching time and cell growth state；The culture apparatus of the present invention is placed in standard incubator, is trained
Support to certain time direct experiment and observation, avoid frequently taking out Tissue Culture Dish, be advantageous to cell growth, and reduce
Workload, and the lead time of observation cell is reduced, increase the accuracy of experimental result.
Control system 20 of the present invention is connected with WIFI or bluetooth module, can realize image remote and wireless transmission, Er Qieyong
Family can realize remote control by man-machine interface and WIFI module, and realization need not open incubator, carry out long-range living cells training
Support the purpose of observation.
CO2Conveying device 30 provides constant CO for cell growth2Concentration, CO2Conveying device 30 includes CO2Inductor 301,
CO2Gas bomb 302, magnetic valve 303, appendix 304, checking device 305, wherein：CO2Inductor 301 is arranged on housing 1, plane
In the side wall of housing 1 in cavity that cover 2 is formed with top cover 8, CO2Inductor 301 can sense CO in the present invention2Concentration, CO2
Gas bomb 302 is arranged on the outside of the present invention, CO2Gas bomb 302 is the flat pressure cylinder of aluminum；Magnetic valve 303 is arranged on this
Invention outside；Magnetic valve 303 can realize that appendix 304 opens or closes, so as to control CO2CO of the conveying device 30 to the present invention2Gas
Conveying；The material of appendix 304 is polytetrafluoroethylene (PTFE), and appendix is by CO2Gas bomb 302 is connected with magnetic valve 303, and then will be defeated
Tracheae 304 is communicated to the inside of the present invention through the side wall of inventive shell 1.
Checking device 305 is arranged on housing 1, plane cover 2 and in the cavity of the formation of top cover 8, checking device 305 includes
CO2Air dispersing hood 3051, CO2Air intake cover 3052, dividing plate 3053, wherein：CO2Air dispersing hood 3051 be arranged on housing 1, plane cover 2 with
Top cover 8 is formed in cavity, and CO2Air dispersing hood 3051 is arranged on the outside of culture dish placement rack 3, the inner side of top cover 8, CO2Air dispersing hood 3051
Size is relative set, CO with the size of top cover 82The surface of air dispersing hood 3051 is provided with some through holes, and through hole runs through CO2Air dispersing hood
3051 are set, CO2Gas can enter CO by through hole2In the cavity that air dispersing hood 3051 forms with plane cover 2, and then reach training
Support the cell culture fluid in the Tissue Culture Dish 502 of ware placement rack 3.CO2Air dispersing hood 3051 has buffered CO2Gas is trained into cell
The impulsive force of nutrient solution, reduce CO2Influence of the gas to biomembrane 503.
In order to further reduce CO2Influence of the gas to biomembrane 503, CO2Air intake cover 3052 can be set to horn-like, CO2
Air intake cover 3052 is arranged on CO2The outside of air dispersing hood 3051, and CO2Air intake cover 3052 is arranged on housing 1, plane cover 2 and top cover 8
Formed in the side wall of cavity inner housing 1, CO2Air intake cover 3052 is small end close to one end of side wall, being held to be big away from side wall, small
End is connected with big end, CO2The small end of air intake cover 3052 is connected with one end of appendix 304, CO2Air intake cover 3052 it is this
Setting can make CO2Gas osculum enters great Kou and gone out, and advantageously reduces wind-force, reduces CO2Influence of the gas to biomembrane 503, and then
Reduce the influence to cell tractive force experimental result；
In order to further reduce CO2Influence of the gas to biomembrane 503, can be in CO2Air intake cover 3052 and CO2Air dispersing hood
Dividing plate 3053 is provided between 3051, the dividing plate 3053 can be by CO2Air intake cover 3052 big end covering, the dividing plate 3053 with
CO2Certain interval is formed between the big end of air intake cover 3052, the lower end of dividing plate 3053 is fixedly connected with plane cover 2, make from
CO2The CO that air intake cover 3052 enters2Gas passes through the stop of dividing plate 3053, reduce further CO2The impulsive force of gas, is avoided
The CO of greater impact power2Gas is directly entered cell culture fluid and biomembrane 503 is had an impact.
Changing liquid automatically device 40 is arranged on the sidepiece 11 of housing 1, plane cover 2 with the cavity of the composition of top cover 8, moving certainly
Liquid device 40 include changing liquid motor 401, change liquid pump first 402, liquid storage bottle 403, waste liquid bottle 404, input pipe 405, efferent duct 406,
Come in and go out joint 407, changes liquid motor 401 and is installed on the centre position of sidepiece 11 of housing 1；It is Wriggling Pump Head to change liquid pump first 402, is changed
Liquid pump first 402 is installed on the plane cover 2 of the sidepiece 11 of housing 1；It is to pass through electricity to change liquid pump first 402 and change liquid motor 401
Arbor is dynamic to be connected, and changes liquid pump first 402 and drives work by changing liquid motor 401；Liquid storage bottle 403 is installed on close to the sidepiece 11 of housing 1
On plane cover 2, and liquid storage bottle 403 is located at the left side for changing liquid pump first 402, and liquid storage bottle 403 is used to store cell culture fluid；Waste liquid
Before bottle 404 is installed on liquid storage bottle 403, waste liquid bottle 404 is used to collect waste liquid.
Input pipe 405 is installed on plane cover 2, and one end of input pipe 405 is by changing liquid pump first 402 and liquid storage bottle 403
Mutually sealing connection；Efferent duct 406 is installed on plane cover 2, and the one end of efferent duct 406 is by changing liquid pump first 402 and waste liquid bottle 404
Mutually sealing connection.
Preferably, input pipe 405 and efferent duct 406 are close to one end part of culture dish and the wall phase of Tissue Culture Dish 502
It should be arranged to bend, make the discrepancy joint of input pipe 405 and efferent duct 406 can be placed in Tissue Culture Dish 502.
Furthermore it is possible to will be close to the input pipe 405 of culture dish and efferent duct 406 is arranged to telescopic structure, it is this to set
The Tissue Culture Dish of different radii size can be applicable by putting.
The joint 407 that comes in and goes out is connected with input pipe 405 and the bending place openend of efferent duct 406.Joint 407 come in and go out in horn-like
Or sucker shape, the joint 407 that comes in and goes out include disk body 4071, discharge conection 4072, input pipe joint 4073 and upper lid 4074, disk body
Gap between 4071 and upper lid 4072 forms cavity volume, and the entirety of disk body 4071 can be set to circular, ellipse even polygon.
The cross section of disk body 4071 is circular, and the longitudinal section of disk body 4071 is step-like, and the upper end of disk body 4071 is small end, under disk body 4071
End is to hold greatly, the center of the small end of disk body 4071 is provided with buffer pool 40711, buffer pool on the upper surface of disk body 4071
40711 outside is provided with some water conservancy diversion projections 40712, and water conservancy diversion projection 40712 is relative to the center of disk body 4071 in uniform
Radial distribution, water conservancy diversion projection 40712 include one-level flow guide bar 407121, two level flow guide bar 407122, three-level flow guide bar
407123, close to the setting of buffer pool, the other end extended at the outer fringe position of disk body for one end of one-level flow guide bar 407121；
Two level flow guide bar 407122 is provided between two sections of adjacent one-level flow guide bars 407121, the length of two level flow guide bar 407122 is short
In one-level flow guide bar 407121, make one end of two level flow guide bar 407122 away from one-level shunt bar 407121 close to buffer pool one
End, the other end are extended at the outer fringe position of disk body；Set between one-level flow guide bar 407121 and two level flow guide bar 407122
There is three-level flow guide bar 407123, three-level flow guide bar 407123 is shorter in length than two level flow guide bar 407122, makes three-level flow guide bar
Away from two level shunt bar 407122 close to one end of buffer pool, the other end extends to the outer fringe position of disk body for 407123 one end
Place；By that analogy, several levels shunt conduit can be set, so far, several levels shunt conduit, disk body are formd between water conservancy diversion projection 40712
Present convex in 4071 lower surface.Buffer pool 40711 is set by annular enclosing 40713 bag and formed, and enclosing 40713 is towards disk body upper table
Extend one section of annular solid upwardly, it can be it is circular can also be other shapes, form cell culture in enclosing 40713
Liquid reservoir, the upper surface of enclosing 40713 is zigzag, and zigzag sets number using the circumferentially-spaced uniform distribution of enclosing
Individual breach of the same size 40714 and formed, the breach 40714 downwards it is recessed it is inside and outside penetrate, and the depth of breach 40714 is less than
The height of enclosing, when the liquid level of cell culture fluid in reservoir exceedes 40714 bottom position of breach, cell culture fluid will
Overflow and flowed on disk body 4071 from breach 40714, cell ware, the water conservancy diversion of the upper surface of disk body 4071 are branched to through shunt conduit
The cell culture fluid that projection 40712 can deliver into input pipe 405 buffer pool 40711 uniformly introduces Tissue Culture Dish 502, drops
To the impulsive force of cell when low cell culture fluid flows into Tissue Culture Dish.
Discharge conection 4072 is located at the upper surface of disk body 4071, and discharge conection 4072 stretches out one section upward, efferent duct
The upper end of joint 4072 is small end, and the upper end of discharge conection 4072 is used to be connected with efferent duct 406, the lower end of discharge conection 4072
It is to hold greatly, the lower ending opening of discharge conection 4072, and the lower end of discharge conection 4072 penetrates the bottom surface of disk body 4071, efferent duct connects
First 4072 lower end is used to draw the waste liquid in Tissue Culture Dish, and the upper end of discharge conection 4072 is than the lower end of discharge conection 4072
It is small.In the case where changing the suction of liquid pump first 402, the setting of discharge conection 4072 and disk body 4071, be advantageous to suction point
Dissipate, it is less or avoid draw cell culture fluid when cell is adsorbed.
The upper covering of lid 4074 is fastened on disk body 4071, and upper lid 4074 just can be by leading positioned at the upper surface of disk body 4071
Stream projection 40712 is completely covered, and lid 4074, disk body 4071 and water conservancy diversion projection 40712 has been collectively constituted cell culture liquid stream
The passage gone out, the center of upper lid 4074 are provided with pipe sleeve 4075, and pipe sleeve 4075 can be socketed in discharge conection 4072, pass through sleeve pipe
4075 can be such that discharge conection 4072 is connected with efferent duct 4076, and input pipe joint 4073, input pipe are provided with sleeve pipe 4075
The center of joint 4073 is provided with the input hole run through up and down, when upper lid is covered on disk body, the just position of input pipe joint 4073
It is connected in the opening position of the reservoir of buffer pool 40712, and with reservoir, cell culture fluid enters liquid storage by input hole
Groove, and then cell culture fluid flows into Tissue Culture Dish through shunt conduit.
Control system 20, which is adopted, computerizeds control, with CO2Inductor 301, magnetic valve 303, change liquid motor 401 and connect；
CO is set on a display screen2Concentration, control system 20 come from CO by receiving2The shell of the present invention that inductor 301 detects
CO in the cavity that body 1, plane cover 2 and top cover 8 form2Concentration, so as to control magnetic valve 303, realize the unlatching of appendix 304
Or close, and then control CO in the cavity that is formed to inventive shell 1, plane cover 2 and top cover 8 of conveying mechanism 302Gas
Conveying, specifically, when control system 20 monitors the CO in the cavity of inventive shell 1, plane cover 2 and the composition of top cover 82
CO of the concentration less than setting2During concentration, control system 20 automatically controls magnetic valve 303 and opened, and makes CO2CO in gas bomb2Through gas transmission
Pipe 304 enters in the cavity that housing 1, plane cover 2 and top cover 8 form, when control system monitors housing 1, the and of plane cover 2
The CO in cavity that top cover 8 forms2When concentration is less than the CO2 concentration set, higher than the CO of setting2During concentration, control system is automatic
Control magnetic valve 303 is closed, and makes CO2CO in gas bomb 3022It cannot pass through appendix 304 and enter inventive shell 1, plane cover
2 and top cover 8 form cavity in.
The present invention is additionally provided with wire clip 501, and electric wire is fixed on inside housing 1.
The operation principle of the present invention：Attached cell is planted on the biomembrane 503 of culture dish bottom, in sterile and CO2
Conveying device provides suitable CO2In the case of environment, the adjustment seat that culture dish is placed on culture dish placement rack 3 by top cover 8 is opened
On lid 31, the screw-cap regulation stretching clamp 41 on alignment jig 41 clamps biomembrane 503, and top cover 8 is covered, by electrode
Line is connected with positive and negative electrode, starts cooling mechanism, culture apparatus inside is radiated so that culture-liquid temp keeps certain
Temperature, the pulling force that control system measures according to pulling force inductor 11 is come the stroke lever 423 of restrained stretching device 4 so as to restrained stretching
The stretching frequency of fixture 41, stretch range, stretching time, by the mode of mechanical stimulation cell close to cell growth, development body
Interior environment, cell growth conditions can be improved, so as to promote cell growth, cytoactive be improved, when the cell of culture certain time
When will change liquid, the running parameters such as flow velocity can be on a display screen set, and launch into working condition, in the course of the work,
Change the band of liquid motor 401 and move liquid pump first 402 and work, flow velocity is after the outflow of liquid storage bottle 403 on request for cell culture fluid, into cell
Culture dish 502, the nutrient solution of outflow are collected to waste liquid bottle 404 automatically.
The above-described embodiments are merely illustrative of preferred embodiments of the present invention, is not to the present invention
Scope is defined, and under the premise of design spirit of the present invention is not departed from, this area ordinary skill technical staff is to the technology of the present invention
Any modification and improvement that scheme is made, it all should fall into the protection domain of claims of the present invention determination.
Claims (10)
1. the cell culture apparatus of dynamic microenvironment in a kind of analogue body, including housing, it is characterised in that also include being arranged at shell
It is used for the clamping device for clamping biomembrane in vivo, the clamping device includes at least two movable clamps, and the biomembrane is located at
Between two fixtures；It is arranged at the stretching device of control clamping device movement in housing；It is provided with and is used between the clamping device
The culture dish placement rack of culture dish holding.
2. the cell culture apparatus of dynamic microenvironment in analogue body according to claim 1, it is characterised in that the stretching
Device includes transmission mechanism, pulling force sensor, and the transmission mechanism includes two fixed seats, stepper motor, screw mandrel and stroke lever,
The stepper motor is fixedly installed in the fixed seat provided with through hole, and the screw mandrel is arranged between two fixed seats, the silk
Bar one end is fixedly connected with the output shaft of motor, and is connected through the through hole of fixed seat, the other end and the screw of another fixed seat
Connect, stroke lever one end is fixedly connected with lower chuck, and the stroke lever other end is provided with screw, and the screw is connected with wire rod thread, described
Stepper motor drives screw mandrel to rotate, and realizes stroke lever horizontal rectilinear motion, and then realizes that stretching clamp moves.
3. the cell culture apparatus of dynamic microenvironment in analogue body according to claim 2, it is characterised in that the pulling force
Sensor is arranged on movable clamp.
4. the cell culture apparatus of dynamic microenvironment in analogue body according to claim 1, it is characterised in that the fixture
Including the adjusting part of upper grip, lower chuck and regulation upper grip movement, upper grip includes upper bare terminal end, upper connecting portion, on
The bottom of connecting portion is connected with upper bare terminal end, and upper connecting portion is in inverted l shape, and lower chuck is arranged on the lower section of upper grip, lower chuck
Including lower bare terminal end, lower connecting portion, upper bare terminal end forms clamping part, for biomembrane to be clamped, lower connecting portion with lower bare terminal end
It is set in parallel in the lower section of connecting portion, the shape of lower connecting portion is the shape identical inverted L shape with upper connecting portion, lower connecting portion
Provided with chute, chute of the upper connecting portion along lower connecting portion slides up and down, so as to drive upper bare terminal end close to or away from lower folder
Hold end, the adjusting part uses adjusting screw rod and nut, by turning adjusting screw rod so as to driving upper bare terminal end close or
Away from lower bare terminal end.
5. the cell culture apparatus of dynamic microenvironment in analogue body according to claim 1, it is characterised in that the culture
Ware placement rack is installed between clamping device, and the culture dish placement rack includes height regulation ring, plummer, height adjustment seat,
The height adjustment seat is fixedly installed on enclosure interior, and the plummer is arranged on height regulation ring lower end or inside, the height
Degree regulation ring is threadedly coupled with height adjustment seat, the adjustment for realizing the height of plummer by turning height regulation ring.
6. the cell culture apparatus of dynamic microenvironment in analogue body according to claim 1, it is characterised in that also including should
Become measurement apparatus, be provided with strain detector on the strain gauge means, the strain detector is foil gauge, the strain
Piece is labelled on biomembrane.
7. the cell culture apparatus of dynamic microenvironment in analogue body according to claim 1, it is characterised in that also include top
Lid and electric stimulation, the electric stimulation include positive and negative electrode, and when top cover covers, the positive and negative electrode is contradicted to biology
On film, the inwall of the top cover is provided with elastic component, and the bottom of the elastic component is fixedly connected with positive and negative electrode.
8. the cell culture apparatus of dynamic microenvironment in analogue body according to claim 1, it is characterised in that also include real
When observing system, the real time viewing system includes CCD camera and the optical fiber that is connected with CCD camera, the optical fiber
It is placed in the top of biomembrane.
9. the cell culture apparatus of dynamic microenvironment in analogue body according to claim 1, it is characterised in that also include flat
Sewing platform base and cooling mechanism, the platform base are fixedly connected below culture dish placement rack, and the cooling mechanism includes at least one
Individual radiator and radiator fan, the radiator upper end are fixedly connected with platform base, the outer end of the radiator and radiation air
One end of fan is connected, and the radiator fan discharges heat by the heat emission hole of housing.
10. the cell culture apparatus of dynamic microenvironment in analogue body according to claim 1, it is characterised in that also include
CO is provided for the cell culture apparatus2CO2Oneself of liquid function is changed in culture dish offer in conveying device, the cell culture apparatus
Move the control system of liquid device and restrained stretching device stretching time, stretching frequency and stretch range.
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