CN207918865U - Clamping device for cell culture and the cell device for precisely stretching using the device - Google Patents
Clamping device for cell culture and the cell device for precisely stretching using the device Download PDFInfo
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- CN207918865U CN207918865U CN201721344695.0U CN201721344695U CN207918865U CN 207918865 U CN207918865 U CN 207918865U CN 201721344695 U CN201721344695 U CN 201721344695U CN 207918865 U CN207918865 U CN 207918865U
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- interconnecting piece
- terminal end
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- clamping
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Abstract
The utility model discloses a kind of clamping devices for cell culture,Including upper grip,Lower collet,And adjust the adjusting part of upper grip movement,Upper grip includes upper bare terminal end,Upper interconnecting piece,The bottom end of upper interconnecting piece is connect with upper bare terminal end,Upper interconnecting piece is in inverted l shape,Lower collet is arranged in the lower section of upper grip,Lower collet includes lower bare terminal end,Lower interconnecting piece,Upper bare terminal end forms clamping part with lower bare terminal end,Lower interconnecting piece is arranged in the lower section of upper interconnecting piece,Upper interconnecting piece is slided up and down along lower interconnecting piece,To drive upper bare terminal end close and separate lower bare terminal end,The adjusting part drives upper clamping part separate and close lower clamping part,Entire fixture is oblique,Biomembrane can be facilitated to put into and take out,The nut that can need not simply turn screw rod by other tools in this way drives upper grip to slide up and down,It is convenient,Rapidly realize clamping or the loose unloading of film,Also disclose a kind of cell device for precisely stretching of the practical clamping device.
Description
Technical field
The utility model is related to technical field of cell culture, are especially for clamping device and the use of cell culture
The cell device for precisely stretching of the device.
Background technology
In organizational project, the external functionalization culture of cell, tissue has become the core technology of an organizational project,
It is the indispensable technical foundation to form organizational project industry.The growth in vitro close with life condition in cell body is provided
Environment, it is most important for the dimensional culture and functionalization of cell, tissue, wherein the physical influences factor such as mechanics, electricity is at it
In play considerable effect.
The experimental method of analogue body interior mechanics environment mainly has Osima jacoti, Osima excavata method, substrate tension method, hydrostatic platen press, circumference
Stress method；The experimental method of the outer mechanical environment of analogue body mainly has microgravity cell culture method, centrifugal force field method, gas pressurized
Method, sound stimulation method, microbeam irradiation method.
Substrate tension technology starts from the appearance of elastic cell's culture membrane.The mentality of designing of substrate tension technology is with biology
Film is as base material, by biomembrane, generating controllable movement by device and making biomembrane cell seeding
Deformation, and cultivate in the cell on biofilm surface due to the deformation of substrate stress, to realize cell growth under tensile stress
The possibility of law study.
With the development of cell research, substrate tension technology to stretch biomembrane clamping device research also increasingly by
Pay attention to.The fixture of the stretching biomembrane of the prior art is not easy to place biomembrane, and takes the time, also adds cell contamination
Possibility, be unfavorable for cell stress research.
Utility model content
In order to solve above-mentioned prior art problem, the utility model provides a convenient, rapidly realizes the clamping of biomembrane
Or a kind of clamping device for cell culture unclamped, including upper grip, lower collet and the adjusting for adjusting upper grip movement
Component, upper grip include upper bare terminal end, upper interconnecting piece, and the bottom end of upper interconnecting piece is connect with upper bare terminal end, and upper interconnecting piece is in inverted L-shaped
Shape, lower collet are arranged in the lower section of upper grip, and lower collet includes lower bare terminal end, lower interconnecting piece, upper bare terminal end and lower bare terminal end shape
At clamping part, for clamping biomembrane, lower interconnecting piece is arranged in the lower section of upper interconnecting piece, and lower interconnecting piece is equipped with sliding slot, upper company
Socket part is slided up and down along the sliding slot of lower interconnecting piece, to drive upper bare terminal end close or separate lower bare terminal end, the adjusting
Component drives upper clamping part separate and close lower clamping part.
In order to further realize the utility model, the lower collet is arranged in parallel with upper grip.
In order to further realize the utility model, the adjusting part is adjusting screw rod, the upper interconnecting piece of the upper grip
Traversing section be equipped with screw hole up and down, the lower collet corresponds to screwhole position equipped with the second through-hole, the bottom end of adjusting screw rod
It is arranged in the second through-hole, the top of adjusting screw rod passes through the screw hole of upper interconnecting piece, adjusting screw rod to be threadedly coupled with upper interconnecting piece,
Upper grip is driven to move up and down by turning adjusting screw rod, to drive the upper bare terminal end of upper grip close and separate lower clamping
End is realized and clamps and unclamp biomembrane.
In order to further realize the utility model, the lower interconnecting piece of the lower collet sets to the concave to be formed along its length
The upper interconnecting piece of sliding slot, the upper grip is slided up and down along sliding slot.
In order to further realize the utility model, the clamping device further includes limiting section, and the lower collet is located at lower company
The position of socket part is provided along its length one section of slide opening, and the upper grip part for corresponding to slide opening position is equipped with through-hole, the limit
Position portion sequentially passes through through-hole and slide opening, and upper interconnecting piece is limited in the slide opening of lower interconnecting piece and slides, avoids by the limiting section
Interconnecting piece is detached from sliding slot, while the distance that upper interconnecting piece is slided is to limit the length of slide opening.
In order to further realize the utility model, the upper grip is inclined to set with lower collet, the upper grip it is upper
The low side of clamping part is connect at an angle with upper interconnecting piece, the angle ranging from obtuse angle, and the upper bare terminal end is arranged with resilient sleeve.
In order to further realize the utility model, the clamping device further includes limiting section, and the lower collet is located at lower company
The position of socket part is provided along its length one section of slide opening, and the length of the slide opening is identical as the screw thread segment length of adjusting screw rod,
It is equipped with through-hole corresponding to the upper grip part of slide opening position, the limiting section sequentially passes through through-hole and slide opening, and the limiting section will
Upper interconnecting piece is limited in sliding in the slide opening of lower interconnecting piece, and interconnecting piece is avoided to be detached from sliding slot, while upper interconnecting piece being slided
Distance is the length of limit slide opening.
A kind of cell device for precisely stretching, including shell further include the clamping dress being set in shell for biomembrane to be clamped
It sets, the clamping device includes at least two movable fixtures, and the movable fixture is used for clamping device, and the biomembrane is located at two
Between a fixture；The stretching device of clamping device movement is controlled in shell；It is equipped between the clamping device for placing
The culture dish placement rack of culture dish.
In order to further realize the utility model, it further includes providing CO for the cell culture apparatus that cell precision, which stretches dress,2
CO2Culture dish in conveying device, the cell culture apparatus provides the changing liquid automatically device for changing liquid function and control stretches dress
Set the control system of stretching time, stretching frequency and stretch range.
Advantageous effect：
1, clamping device provided by the utility model includes upper grip, lower collet and the adjusting for adjusting upper grip movement
Component, upper grip include upper bare terminal end, upper interconnecting piece, and the bottom end of upper interconnecting piece is connect with upper bare terminal end, and upper interconnecting piece is in inverted L-shaped
Shape, lower collet are arranged in the lower section of upper grip, and lower collet includes lower bare terminal end, lower interconnecting piece, upper bare terminal end and lower bare terminal end shape
At clamping part, for clamping biomembrane, lower interconnecting piece is arranged in parallel in the lower section of interconnecting piece, the shape of lower interconnecting piece be with
The identical inverted L shape of upper interconnecting piece, lower interconnecting piece are equipped with sliding slot, and upper interconnecting piece is slided up and down along lower interconnecting piece sliding slot, adjusting group
Part uses adjusting screw rod and nut；By turning adjusting screw rod to drive upper bare terminal end close and separate lower bare terminal end, with reality
The clamping of existing biomembrane and loose unloading, this is simple in structure, convenient, quickly.
2, the upper interconnecting piece of the utility model forms certain angle with upper bare terminal end, and lower interconnecting piece forms one with lower bare terminal end
Determine angle, angle is preferably obtuse angle so that entire fixture is oblique, and vertical structure, this set are used relative to traditional
On the one hand Tissue Culture Dish side wall is avoided, it is more convenient in culture dish holding, fixture will not be caused to touch culture dish, favorably
In the smooth culture of cell；On the other hand being obliquely installed can facilitate biomembrane to put into and take out, in this way need not be by other works
Tool, so that it may which the nut simply to turn screw rod drives upper grip to slide up and down, and easily and quickly realizes clamping or the pine of biomembrane
It unloads.
3, the utility model close to lower bare terminal end by being equipped with one section of limit slide opening on the chute, and limit slide opening is along inclination
Partial-length direction is arranged, and the utility model, which corresponds in the upper interconnecting piece of upper grip at limit slide opening, is equipped with through-hole, limiting section
Through-hole and slide opening are sequentially passed through, upper interconnecting piece is limited in the sliding slot of lower interconnecting piece and slides by limiting section, avoids interconnecting piece de-
From sliding slot, and it can avoid upper grip and shake, cannot accurately clamp biomembrane, while the distance that upper interconnecting piece is slided is that limit is slided
The length in hole, when limiting section slides into the bottom end of limit hole, upper bare terminal end at this time is fitted tightly on lower bare terminal end.
4, the utility model additionally provides the cell tensile precision apparatus of practical clamping device, by being equipped with inside housings
Plane cover, the center of plane cover, which is equipped with, places bore, is equipped with multiple dragging tracks around bore along placing, each slides rail
It is set on road there are one fixture, biomembrane (basilar memebrane) is clamped in fixture, and fixture is moved up by stepper motor in dragging track
Dynamic, fixture is equipped with the pulling force sensor of detection biomembrane pulling force, and sends information to control system, to control stepping electricity
Machine, the horizontal stress load of step motor control fixture, it is controllable to realize pulling force, and can control different stress intensities to thin
The influence of born of the same parents, and the fixture of the utility model is arranged along culture dish placement rack to the direction of ambient radiation, and fixture may be provided at
It is multi-direction, it can be achieved that carry out unidirectional, two-way, multidirectional cyclic tension to biomembrane, and controllable stretching time to biomembrane,
Stretching frequency and stretch range；Traditional applies positive air pressure or negative pressure to realize biomembrane by the bottom to biomembrane
In the method for deformation, the mechanical stimulation that cell is subject to is non-uniform, and the size of power is uncontrollable, and it is quantitative not really to there is experiment
Qualitative, causing experimental design, there are certain error or deficiencies.And the utility model stretching clamp is provided with pulling force inductor,
Stretching clamp can be arranged in multiple directions, and pulling force inductor can measure the stress intensity of respective direction, then according to cell
Growth conditions, adjust the pulling force of respective direction in time, it can be achieved that the pulling force of multiple directions either synchronously or asynchronously adjusts, can be real
Existing different stress, the stimulation of different directions；Pulling force inductor can measure the stress to biomembrane accordingly, and it is quantitative to avoid experiment
Uncertainty, obtain more accurate experimental result.
5, the utility model is additionally provided with head cover, and the growth for being set as cell of head cover provides pollution-free, anti-culture medium and steams
Hair, the environment of humidity and proper temperature.Tissue Culture Dish can be with direct experiment and culture, no in the incubator of the utility model
Lid culture ware lid, the setting that culture dish is combined with incubator is needed to simplify experimental procedure, save the time, and avoid
Lead to pollution and the influence to cell growth state because frequently taking out Tissue Culture Dish.
6, the culture dish placement rack of the utility model can realize the control of the height of culture dish by rotation height regulation ring
System, since when doing tension test, fixture should be as possible close to culture dish bottom, and general culture solution is more expensive, when culture dish position
When setting too low, generally by adding culture solution come so that fixture bare terminal end is immersed in culture solution so that basilar memebrane can be complete
It is immersed in culture solution, the utility model can up adjust to avoid addition culture solution by adjusting culture dish, to also may be used
To avoid the waste of culture solution.
Description of the drawings
Fig. 1 is the utility model clamping device overall schematic；
Fig. 2 is the structural schematic diagram of the utility model upper grip；
Fig. 3 is the structural schematic diagram of collet under the utility model；
Fig. 4 is the structural schematic diagram of the utility model limiting section；
Fig. 5 is the cell device for precisely stretching structural schematic diagram using the grip device；
Fig. 6 is the structural schematic diagram of the stretching device of Fig. 5；
Fig. 7 is the structural schematic diagram of head cover；
Fig. 8 is the control principle drawing of real time viewing system；
Fig. 9 is the structural schematic diagram that the utility model does not install clamping device and head cover；
Figure 10 is the structural schematic diagram after Fig. 9 installs fixtures；
Figure 11 is the overall structure diagram after top cover；
Figure 12 is the CO of the utility model2The structural schematic diagram of conveying mechanism；
Figure 13 is the changing liquid automatically schematic device of the utility model；
Figure 14 is the structural schematic diagram of the changing liquid automatically device discrepancy connector of the utility model；
Figure 15 is the sectional view of the changing liquid automatically device discrepancy connector of Figure 14 the utility model；
Figure 16 is the vertical view of the changing liquid automatically device discrepancy connector of Figure 14 the utility model.
Specific implementation mode
The utility model is further elaborated with reference to the accompanying drawings and examples, these attached drawings are simplified signal
Figure, only illustrates the basic structure of the utility model, the direction of this specific implementation is using the directions Fig. 1 as standard in a schematic way.
As shown in Figure 1, the utility model discloses a kind of cell clamping device for cell culture mechanical stretch, clamp
Device 41 includes upper grip 411, lower collet 412, adjusting part 413, wherein upper grip 411 includes upper bare terminal end 4111, upper company
Socket part 4112, upper bare terminal end 4111 are a slab construction, and upper bare terminal end 4111 is arranged with resilient sleeve (not shown), avoid because
Upper bare terminal end 4111 and biomembrane hard contact and damage biomembrane, the bottom end of upper interconnecting piece 4112 and 4111 one of upper bare terminal end
It connects or is fixedly connected, upper interconnecting piece 4112 is inverted l shape, wherein the longitudinal component and upper bare terminal end of upper interconnecting piece 4112
It 4111 and connects at an angle, preferably obtuse angle so that entire upper grip 411 is inclined to set, and tilting section is equipped with through-hole
41121, upper interconnecting piece 4112 is located at the lateral part on top equipped with screw hole 41122 up and down；
Lower collet 412 is arranged in the lower section of upper grip 411, and lower collet 412 includes lower bare terminal end 4121, lower interconnecting piece
4122, and lower bare terminal end 4121 is arranged in parallel with upper bare terminal end 4111, upper bare terminal end 4111 is formed with lower bare terminal end 4121 and is clamped
Portion, for clamping biomembrane, lower bare terminal end 4112 is arranged with resilient sleeve (not shown), avoids because of biomembrane and lower folder
It holds 4121 hard contacts of end and damages biomembrane；Lower interconnecting piece 4122 is arranged in parallel in the lower section of interconnecting piece 4112, lower connection
Portion 4122 is inverted l shape identical with 4112 shape of upper interconnecting piece, and the entire sloping portion of lower interconnecting piece 4122 is set to the concave into
Sliding slot 41221, upper interconnecting piece 4112 slide up and down in sliding slot 41221, to drive upper bare terminal end 4111 close to far under
Bare terminal end 4121, and then realize clamping and loose unloading biomembrane, on sliding slot 41,221 one section of limit is equipped with close to lower bare terminal end 4121
Slide opening 41222, limit slide opening 41222 are arranged along sloping portion length direction, and the utility model is equipped in through-hole 41121
Upper interconnecting piece 4112 is limited in the sliding slot of lower interconnecting piece 4122 and slides by limiting section 4113, limiting section 4113, avoids connection
Portion 4112 is detached from sliding slot, and can avoid upper grip and shake, and cannot accurately clamp biomembrane, while upper interconnecting piece 4112 being slided
Distance is limits the length of slide opening 41222, when limiting section 4113 slides into the bottom end of limit slide opening 41222, upper folder at this time
End 4111 is held to be fitted tightly on lower bare terminal end 4121；The banking pin that upper and lower ends carry cap, limit can be used in limiting section 4113
The head of a nail across 41121 bottom end of first through hole of nail is slidably installed in the both ends of limit hole；As shown in fig. 6, can also be used with lower structure
It realizes, including upper and lower two fixed blocks 41131, and is connected to the connection bar portion 41132 of two fixed blocks 41131, upper fixation
The width of block 41131 is more than first through hole 41121, and the width of lower fixed block 41131 is more than the width of limit slide opening 41222, even
Reach bar portion passes through first through hole 41121, upper and lower two fixed blocks 41131 to be fitted closely respectively in upper interconnecting piece 4112 and lower connection
The sliding in portion 4122, upper interconnecting piece 4112 drives lower fixed block 41222 to be slided along limit slide opening 41222；Lower interconnecting piece 4122
Lateral part be equipped with the second through-hole 41223 up and down, adjusting part 413 includes adjusting screw rod 4131, nut 4132, is adjusted
The bottom end of section screw rod 4131 is rotatably provided in the second through-hole 41223, and the top of adjusting screw rod 4131 passes through upper interconnecting piece
4112 screw hole, and be fixedly connected with nut 4132, adjusting screw rod 413 is threadedly coupled with upper interconnecting piece 4112, preferred to implement
In mode, the length of adjusting screw rod 413 is identical as the limit length of slide opening 41222, turns nut 4132, and nut 4132, which drives, to be adjusted
It saves screw rod 4131 to rotate, so that upper grip 411 moves up and down, and then adjusts upper bare terminal end 4111 and lower bare terminal end 4121
Distance, further realize the function of clamping and loose unloading elastic substrates film, to realize clamping and the loose unloading of basilar memebrane, structure letter
It is single, it is convenient, quickly.
The fixture of the utility model is obliquely installed, on the one hand this set avoids Tissue Culture Dish side wall, is trained placing
It is more convenient when foster ware, fixture will not be caused to touch culture dish, be conducive to the smooth culture of cell；On the other hand being obliquely installed can
Biomembrane is facilitated to put into and take out, the nut that can need not simply turn screw rod by other tools in this way drives upper grip
It slides up and down, easily and quickly realizes clamping or the loose unloading of film.
The invention also discloses a kind of cell device for precisely stretching using the fixture, it includes shell 1, plane face
Lid 2, culture dish placement rack 3, clamping device 41, stretching device 4, platform base 5, cooling mechanism 6, head cover 7, real time viewing system
8, control system 9, CO2Conveying mechanism 10, changing liquid automatically device 20 and display screen (not shown), wherein：
The opening-like structure in 1 upper end of shell is in square or cuboid, is certainly not limited to this shape, and the one of shell is lateral
On convex to form a side 11,1 inside of shell forms cavity, plane cover 2, culture dish placement rack 3, stretching device 4, platform base
Seat 5, cooling mechanism 6 are arranged in cavity；
Plane cover 2 is arranged at the upper opening of shell 1, and plane cover 2 is adapted with shell 1, plane cover 2 and shell
Body 1, which can be screwed or block, sets connection, and the upper surface of plane cover 2, which is provided centrally with, places bore 21, places bore 21
In being arranged up and down, it is circle to place bore 21, and number is equipped at intervals with outward along the circumferential edge for placing bore in plane cover 2
A sliding shape track 22, sliding shape track 22 is in strip, and slides the plane cover 2 up and down of shape track 22.
Culture dish placement rack 3 is used to adjust the height of culture dish, including height regulation ring 31, plummer 32, height are adjusted
Seat 33.The setting of height regulation ring 31 is being placed at bore 21, and height regulation ring 32 is in circular ring shape, and 31 outer wall of height regulation ring is equipped with
Ring shaped thread；And the upper surface of height regulation ring 32 is set there are two notch 311, is convenient for rotation height regulation ring 31, plummer 32
It is fixed at the bottom end of height regulation ring 31, or is embedded inside regulation ring 31, to form a loading floor, is held
Microscope carrier 32 is rounded, which is used for culture dish holding 302, and height adjustment seat 33 is in hollow cylindric, height adjusting
33 inner walls of seat are equipped with ring shaped thread, and 33 internal thread of height adjustment seat coordinates with 31 external screw thread of height regulation ring, and rotation in this way is high
The height of adjustable carrier platform 32 when spending regulation ring 31, the bottom end of height adjustment seat 33 is fixedly connected with platform base 6；
The culture dish placement rack 3 of the utility model can realize the height control of plummer 32 by rotation height regulation ring 31
System, and then realize the height control of culture dish 302, due to when doing tension test, fixture 41 should as possible close to culture dish bottom,
General culture solution is more expensive, when 302 position of culture dish is too low, generally by adding culture solution come so that fixture bare terminal end
It is immersed in culture solution so that basilar memebrane can be completely submerged in culture solution, and the utility model can be by adjusting culture dish
302 up adjust to avoid addition culture solution, to avoid the waste of culture solution.
Clamping device 41 can clamp biomembrane, and clamping device 41 is at least two movable fixtures, and each dragging track pair
It should set there are one fixture 41, the utility model sets at least to two, and the utility model is for four, and at each two fixture
It is arranged in same straight line, clamping device 41 uses the structure of above-mentioned cell clamping device.
4 stretchable cell biological film of stretching device, stretching device 4 include control clamping device movement transmission mechanism 42 with
And pulling force sensor 43, wherein；
Transmission device 42 includes two fixed seats 421, stepper motor 422, lead screw 423 and stroke lever 424；Two fixed seats
421 parallel longitudinal are fixed on platform base 6；One of fixed seat 421 is equipped with through-hole 4211, and 4211 size of through-hole is big
In the diameter of lead screw, another fixed seat corresponds to lead to the hole site and is equipped with screw hole 4212, and stepper motor 422 is fixed at equipped with logical
In the fixed seat 421 in hole, lead screw 422 is arranged between two fixed seats 421, the output of lead screw 422 one end and stepper motor 422
Axis is fixedly connected, and across the through-hole of fixed seat, the other end connect with the screw hole of another fixed seat, 424 one end of stroke lever and
Lower collet 412 is fixedly connected, and can be fixed by screw, and 424 other end of stroke lever is equipped with screw hole, the screw hole and 423 screw thread of lead screw
Connection, stepper motor 422 drive lead screw rotation, realize 424 horizontal rectilinear motion of stroke lever, and then realize stretching clamp 41 along
The dragging track 22 of plane cover 2 moves, and upper and lower collet can be driven to do horizontal rectilinear motion to the cell on biomembrane, so as to
Load stretching is carried out to the cell of attachment, simulates internal cell, tissue stretching force environment, is the growth in vitro of cell, tissue
Research provide condition.
It selects stepper motor 422 that can control the precision often walked in 3%-5%, and the mistake of a step will not be heaped and be arrived
In next step, therefore there is the repeatability of preferable bearing accuracy and movement.
Pulling force inductor 43 is arranged on the holder 41, may be provided on upper grip or lower collet, pulling force sensor 43 can
It detects stress suffered by biomembrane, and sends information to control system, to control stepper motor, step motor control folder
The horizontal stress of tool loads, and realizes that pulling force is controllable, and can control influence of the different stress intensities to cell, this practicality is newly
The setting of type fixture 41 is arranged in 3 ambient radiation direction of culture dish placement rack, and fixture 41 may be provided at multiple directions, this set
It can control unidirectional, two-way, multidirectionally and cyclic tension, and controllable stretching time, stretching frequency to biomembrane are carried out to biomembrane
Rate and stretch range.
If not adding pulling force inductor 43, such as positive air pressure or negative pressure are applied to realize life by the bottom to biomembrane
The deformation of object film realizes the stimulation to cell with this, and the stimulation of power that this method cell is subject to is non-uniform, and the size of power
It is uncontrollable, exist and test quantitative not confirmability, causing experimental design, there are certain error or deficiencies.And this practicality is new
Type stretching clamp 41 is provided with pulling force inductor 43, and stretching clamp 41 can be arranged in multiple directions, and pulling force inductor 43 can be with
The stress intensity of respective direction is measured, then according to the growth conditions of cell, adjusts the pulling force of respective direction in time, it can be achieved that more
The pulling force in a direction either synchronously or asynchronously adjusts, and can realize different stress, the stimulation of different directions；Pulling force inductor 43 can be surveyed
It obtains to the stress of biomembrane, to avoid accordingly and tests quantitative uncertainty, obtain more acurrate experimental result.
Platform base 5 is arranged in the lower section of stretching device 4, and the center of platform base 5 corresponds to culture dish placement rack 3
Position be equipped with third through-hole 51, and its periphery is equipped with several through-holes, and heat can pass from through-hole.
Cooling mechanism 6 is arranged in the lower section of platform base 5, and cooling mechanism 6 includes radiator 61 and radiator fan 62, heat dissipation
The upper end of device 61 is fixedly connected with platform base 6, and the outer end of radiator 61 is connected with one end of radiator fan 62, and radiator 61 can
To collect the heat generated when incubator work, heat emission hole of the heat through shell that radiator fan 62 can collect radiator 61
And be discharged outside shell, the temperature of the culture solution in culture dish 302 is within the scope of the thermostat temperature being specifically arranged, and is carried for cell
For suitable temperature environment, it is ensured that experiment is smoothed out.
The shapes and sizes of head cover 7 are adapted with shell 1, and the lower end of head cover 7 is arranged in opening, the surrounding and shell of head cover 7
(card is set) is arranged on shell 1 in body upper end sealed set, head cover 7, the growth for being set as cell of head cover 7 provide it is pollution-free,
Anti- culture medium evaporation, the environment of humidity and proper temperature.Tissue Culture Dish 302 can be straight in the culture apparatus of the utility model
Experiment and culture are connect, culture ware lid need not be covered, the setting that culture dish is combined with incubator simplifies experimental procedure, saves
Time, and avoid leading to pollution and influence to cell growth state because frequently taking out Tissue Culture Dish.
Real time viewing system 8 includes CCD camera 81, optical fiber 82, the first data transmit-receive module 83, the second data transmit-receive
Module 84.Optical fiber 82 is connect with CDD cameras 81.CCD camera 81, be arranged on head cover 7 or the side of shell 1 11 on,
Also it can be mounted on cell incubator, and optical fiber 82 is suspended from the top of culture dish 302, CCD camera 81 and the second data transmit-receive
Module 82 be connected, the first data transmit-receive module 94 with the second data transmit-receive module 84 by being wirelessly connected, the first data transmit-receive mould
Block 83 is connected with control system 9, and the cell image of imaging is transmitted to the first data transmit-receive mould by CCD camera 81 by optical fiber 82
Data information is sent to the second data transmit-receive module 84 and then is stored by control system 9 by block 84, the first data transmit-receive module 83
Display.
The image of Tissue Culture Dish is transmitted to outside cell incubator by optical fiber 81, and can be clearly observable by eyepiece
The form of cell, in the other end of optical fiber 81, the image being transmitted through with 81 reception optical fiber of CCD camera, through CCD camera
After dsp processor is acquired and stores to figure, the first radio receiving transmitting module is emitted to by the second radio receiving transmitting module 84
On 83, be resent on control system computer, in case observation in real time, storage or image procossing, computer by image procossing,
The also authenticity of original image analyzes the cultivation conditions of cell, and prompts certain important stages so that work people
Member can observe on specific time, analyze the state of cell.
CO2Conveying mechanism 10 provides constant CO for cell growth2Concentration, CO2Conveying mechanism 10 includes CO2Inductor 101,
CO2Storage bottle 102, solenoid valve 103, appendix 104, checking device 105.
CO2Inductor 101 is arranged in the housing sidewall in the cavity that shell 1, plane cover 2 are formed with head cover 7, CO2Sense
Answer device 101 that can incude CO in the utility model2Concentration, CO2Storage bottle 102 is arranged in the outside of the utility model, CO2Storage
Bottle 102 is the flat pressure cylinder of aluminum；Solenoid valve 103 is arranged on the outside of the utility model；Solenoid valve 103 can realize on or off from
And control CO2CO of the conveying mechanism 10 to the utility model2The conveying of gas；104 material of appendix is polytetrafluoroethylene (PTFE), gas transmission
CO2 storage bottles 102 are connected by pipe with solenoid valve 103, and then appendix 104 is connected to across 1 side wall of the utility model shell
The inside of the utility model.
Checking device 105 is arranged in the cavity that shell 1, plane cover 2 are formed with head cover 7, and checking device 105 includes
CO2Air dispersing hood 1051, CO2Air intake cover 1052, partition board 1053.
CO2The setting of air dispersing hood 1051 is formed in shell 1, plane cover 2 and head cover 7 in cavity, and CO2Air dispersing hood 1051 is set
It sets in 3 outside of culture dish placement rack, 7 inside of head cover, CO2Air dispersing hood 1051 is with 7 phase of head cover suitable for setting, CO21051 table of air dispersing hood
Face is provided with several through-holes, and through-hole runs through CO2Air dispersing hood 1051 is arranged, CO2Gas can enter CO by through-hole2Air dispersing hood 1051
In the cavity formed with plane cover 2, and then reach the cell culture being placed in the Tissue Culture Dish 302 of culture dish placement rack 3
Liquid.CO2Air dispersing hood 1051 has buffered CO2Gas enters the impact force of cell culture fluid, reduces CO2Influence of the gas to biomembrane.
In order to further decrease CO2Influence of the gas to biomembrane, CO2Air intake cover 1052 can be set to horn-like, CO2Into
Fan housing 1052 is arranged in CO21051 outside of air dispersing hood, and CO2The setting of air intake cover 1052 is in shell 1, plane cover 2 and 7 shape of head cover
In coelosis body on the side wall of shell, CO2Air intake cover 1052 is small end by one end of proximal wall, is big end, small end far from side wall
It is connected with big end, CO2The small end of air intake cover 1052 can be connected with one end of appendix 104, CO2Air intake cover 1052 it is this
Setting can make CO2Gas osculum goes out into great Kou, advantageously reduces wind-force, reduces CO2Gas is reduced the influence of biomembrane pair
The influence of cell tractive force experimental result.
In order to further reduce CO2Gas, can be in CO by the influence of biomembrane2Air intake cover 1052 and CO2Air dispersing hood
Partition board 1053 is provided between 1051, the partition board 1053 can be by CO2The big end of air intake cover 1052 covers, the partition board 1053 with
CO2Certain interval is formed between the big end of air intake cover 1052,1053 lower end of the partition board is fixedly connected with plane cover 2, make from
CO2The CO that air intake cover 1052 enters2Gas further alleviates CO by the blocking of partition board 10532The impact force of gas, avoids
The CO of greater impact power2Gas directly passes in and out cell culture fluid so that being had an impact to biomembrane.
Changing liquid automatically device 20 is arranged in the cavity that the side of shell 1 11, plane cover 2 are formed with head cover 7, moves certainly
Liquid device 20 include change liquid motor 201, change liquid pump head 202, liquid storage bottle 203, waste liquid bottle 204, input pipe 205, efferent duct 206,
Come in and go out connector 207, changes liquid motor 201 and is installed on 11 centre position of side of shell 1；It is Wriggling Pump Head to change liquid pump head 202,
Liquid pump head 202 is changed to be installed on the plane cover 2 of 1 side 11 of shell；It is to pass through that liquid pump head 202, which is changed, with liquid motor 201 is changed
Motor shaft is dynamic to be connected, and changes liquid pump head 202 by changing liquid motor 201 and drives work；Liquid storage bottle 203 is installed on close to 1 side 11 of shell
Plane cover 2 on, and storage bottle 203 is located at and changes the left side of liquid pump head 202, and liquid storage bottle 203 is for storing cell culture fluid；It is useless
Before liquid bottle 204 is installed on storage bottle 203, waste liquid bottle 204 is for collecting waste liquid.
Input pipe 205 is installed on plane cover, and one end of input pipe 205 is by changing liquid pump head 202 and storage bottle 203
Mutually sealing connection；Efferent duct 206 is installed on plane cover 2, and 206 one end of efferent duct is by changing liquid pump head 202 and waste liquid bottle
The sealing connection of 204 phases
Preferably, 302 shape of one end part suitable cell culture dish of input pipe 205 and efferent duct 206 close to culture dish
At bending, input pipe 205 and efferent duct 206 are stretched by the connector that comes in and goes out in Tissue Culture Dish 302.
Furthermore it is possible to will be close to the input pipe 205 of culture dish and efferent duct 206 is arranged to telescopic structure, it is this to set
The culture dish of different radii size can be applicable in by setting.
The connector 207 that comes in and goes out is connected with input pipe 205 and 206 bending place open end of efferent duct.Connector 207 come in and go out in horn-like
Or sucker shape, the connector 207 that comes in and goes out include disk body 2071, discharge conection 2072, input pipe joint 2073 and upper cover 2074, disk
Gap between body 2071 and upper cover 2072 forms vessel, and the entirety of disk body 2071 can be set to round, ellipse or even polygon
Shape.The cross section of disk body 2071 is round, and the longitudinal section of disk body 2071 is step-like, and 2071 upper end of disk body is small end, disk body
2071 lower ends are big ends, the center of 2071 small end of disk body are provided with buffer pool 20711, on 2071 upper surface of disk body
The outside of buffer pool 20711 is provided with several water conservancy diversion protrusions 20712, center of the water conservancy diversion protrusion 20712 relative to disk body 2071
In uniform radial distribution, water conservancy diversion protrusion 20712 is led including level-one flow guide bar 207121, two level flow guide bar 207122, three-level
Flow item 207123, close to the setting of buffer pool, the other end extends to the outer fringe position of disk body for one end of level-one flow guide bar 207121
Place；It is provided with two level flow guide bar 207122 between two sections of adjacent level-one flow guide bars 207121, two level flow guide bar 207122
It is shorter in length than level-one flow guide bar 207121, makes one end of two level flow guide bar 207122 far from the close buffering of level-one shunt bar 207121
The one end in pond, the other end extend at the outer fringe position of disk body；Level-one flow guide bar 207121 and two level flow guide bar 207122 it
Between be provided with three-level flow guide bar 207123, three-level flow guide bar 207123 is shorter in length than two level flow guide bar 207122, three-level is made to lead
Far from two level shunt bar 207122 close to one end of buffer pool, the other end extends to the outer rim of disk body for one end of stream item 207123
At position；And so on, several levels shunt conduit can be set, so far, several levels shunt conduit is formd between water conservancy diversion protrusion 20712,
Present convex in the lower surface of disk body 2071.Buffer pool 20711 is set by the packet of annular enclosing 20713, and enclosing 20713 is towards disk body
Upper surface upwardly extends one section of annular solid, and it can also be other shapes that it, which can be circular, and cell is formed in enclosing 20713
The upper surface of culture solution reservoir, enclosing 20713 is zigzag, and zigzag is set using the circumferentially-spaced uniform distribution of enclosing
It sets several notches of the same size 20714 and is formed, the notch 20714 recessed inside and outside perforation, and the depth of notch 20714 downwards
Degree is less than the height of enclosing, and when the liquid level of cell culture fluid in reservoir is more than 20714 bottom position of notch, cell is trained
Nutrient solution will be overflowed from notch 20714 and be flowed on disk body 2071, and cell ware, 2071 upper table of disk body are branched to through shunt conduit
The cell culture fluid that input pipe can be delivered into buffer pool 20711 by the water conservancy diversion protrusion 20712 in face uniformly introduces Tissue Culture Dish
302, reduce impact force of the cell culture fluid to cell.
Discharge conection 2072 is located on the upper surface of disk body 2071, and discharge conection 2072 stretches out one section upward, output
2072 upper end of pipe fitting is small end, for being connected with efferent duct 206；2072 lower end of discharge conection is big end, and efferent duct connects
First 2072 lower ending opening, and 2072 lower end of discharge conection penetrates the bottom surface of disk body 2071,2072 lower end of discharge conection is used for
The waste liquid in Tissue Culture Dish is drawn, 2072 upper end of discharge conection is smaller than 2072 lower end of discharge conection.Changing liquid pump head 202
Suction under, the setting of discharge conection 2072 and disk body 2071 is conducive to disperse suction, less or avoid drawing
Cell is adsorbed when cell culture fluid.
The covering of upper cover 2074 is fastened on disk body 2071, and upper cover 2074 just can be by leading positioned at 2071 upper surface of disk body
Stream protrusion 20712 is completely covered, and upper cover 2074, disk body 2071 and water conservancy diversion protrusion 20712 is made to have collectively constituted cell culture liquid stream
The center in the channel gone out, upper cover 2074 is equipped with pipe sleeve 2075, and pipe sleeve 2075 can be socketed in discharge conection 2072, pass through casing
2075 can be such that discharge conection 2072 is connect with efferent duct 2076, and input pipe joint 2073, input pipe are provided on casing 2075
The center of connector 2073 is provided with perforative input hole up and down, when upper cover is covered on disk body, the just position of input pipe joint 2073
It is connected at the position of the reservoir of buffer pool 20712, and with reservoir, cell culture fluid enters liquid storage by input hole
Slot, and then cell culture fluid flows into Tissue Culture Dish through shunt conduit.
Control system 9, which is adopted, computerizeds control, with stretching device 4, pulling force sensor 43, CO2Inductor 101, solenoid valve
103, the connection of liquid motor 201 is changed；Display screen is arranged on 1 side wall of shell, and display screen is connect with control system 9.
When the control transmission device 42 of control system 9 and pulling force inductor 43 can determine stretching frequency, stretch range, stretch
Between；Stretching frequency, stretch range, stretching time are set on a display screen, tests, can be drawn by the stretching of certain time
The relationship of power, stretching frequency, stretch range and stretching time and cell growth state；
Stretching device 4, control system 9 are combined applied to making cell culture experiments simpler, conveniently in cell culture,
It saves the time and experiment is more acurrate.
When doing cell experiment, such as the irritant test of cell, if it is inquiring into different incentive conditions to cell growth shape
The influence of state, workload is bigger when experimental implementation, biological because strictly speaking experiment includes that biology repeats and experiment repeats
It learns and repeats to be exactly one group of experiment same batch cell, generally require 3 wares, experiment repeats to refer to identical experiment different batches, i.e., not
Same experiment number, it is 3 times general (different batches cell), there are 3 levels (identical frequency of stimulation, thorn if it is incentive condition is inquired into
The sharp time divides 10min, 30min and 1h) so identical frequency of stimulation, the experiment of different stimulated time will need 9 wares, 93, wares batch
Secondary cytositimulation difference 10min, 30min and 1h take out observation cell growth state, and workload is bigger when experimental implementation, and sees
It examines time meeting difference, take out the more growth conditions that can also influence cell of number, these all may cause experimental error larger.
Control system 9 in the culture apparatus of the utility model can be with by controlling transmission device 42 and pulling force inductor 43
Determine stretching frequency, stretch range, stretching time；By certain time stretching test, can obtain pulling force, stretching frequency,
The relationship of stretch range and stretching time and cell growth state；The culture apparatus of the utility model is placed on standard incubator
In, it cultivates to certain time direct experiment and observation, avoids frequently taking out Tissue Culture Dish, be conducive to cell growth, and
Workload is reduced, and reduces the lead time of observation cell, increases the accuracy of experimental result.
CO is set on a display screen2Concentration, control system 9 come from CO by reception2Inductor 101 detects the utility model
CO in the cavity that shell 1, plane cover 2 and head cover 7 form2Concentration realize appendix 104 to control solenoid valve 103
It is turned on and off, and then controls in the cavity that conveying mechanism 10 forms the utility model shell 1, plane cover 2 and head cover 7
CO2The conveying of gas, specifically, when control system 9 monitors that the utility model shell 1, plane cover 2 and head cover 7 form
Cavity in CO2CO of the concentration less than setting2When concentration, control system 9 automatically controls the unlatching of solenoid valve 103, makes in storage bottle
CO2Enter cell culture apparatus through appendix 104, when control system monitors the chamber that shell 1, plane cover 2 and head cover 7 form
Internal CO2CO of the concentration less than setting2When concentration, higher than the CO of setting2When concentration, control system automatically controls solenoid valve 103
It closes, makes CO in storage bottle 1022It cannot pass through appendix 104 and enter 7 groups of the utility model shell 1, plane cover 2 and head cover
At cavity in.
The utility model is additionally provided with wire clip 301, and electric wire is fixed on inside shell 1.
The operation principle of the utility model：Attached cell is planted on the biomembrane of culture dish bottom, in sterile and CO2
Conveying mechanism provides suitable CO2In the case of environment, the adjustment seat that culture dish is placed on culture dish placement rack 3 by head cover 7 is opened
On lid 31, the screw-cap on alignment jig 41 adjusts stretching clamp 41 and clamps biomembrane, and head cover is covered, and the present apparatus is put
It is placed in cell incubator, and electrode wires is connect with positive and negative electrode, start cooling mechanism, culture apparatus inside is dissipated
Heat so that culture-liquid temp keeps certain temperature, the pulling force that control system is measured according to pulling force inductor 11 to be stretched to control
The stroke lever 423 of device is to control stretching clamp stretching frequency, stretch range, stretching time, by the side of mechanical stimulation cell
Formula can improve cell growth conditions close to cell growth, the vivo environment of development, to promote cell growth, improve cell
Activity, when cultivate certain time cell will change liquid when, the running parameters such as flow velocity can be set on a display screen, and start into
Enter working condition, during the work time, changes 201 band of liquid motor and move the work of liquid pump head 202, cell culture fluid flow velocity as required
After the outflow of liquid storage bottle 203, into Tissue Culture Dish 302, the culture solution of outflow is collected automatically to waste liquid bottle 204.
Embodiment described above is only that preferred embodiments of the present invention are described, and is not to this reality
It is defined with novel range, under the premise of not departing from the spirit of the design of the utility model, this field ordinary engineering and technical personnel
To any modification and improvement that technical solutions of the utility model are made, claims determination of the utility model should all be fallen into
In protection domain.
Claims (9)
1. a kind of clamping device for cell culture, including upper grip, lower collet and the adjusting group for adjusting upper grip movement
Part, upper grip include upper bare terminal end, upper interconnecting piece, and the bottom end of upper interconnecting piece is connect with upper bare terminal end, and upper interconnecting piece is in inverted L-shaped
Shape, lower collet are arranged in the lower section of upper grip, and lower collet includes lower bare terminal end, lower interconnecting piece, upper bare terminal end and lower bare terminal end shape
At clamping part, for clamping biomembrane, lower interconnecting piece is arranged in the lower section of upper interconnecting piece, and lower interconnecting piece is equipped with sliding slot, upper company
Socket part is slided up and down along the sliding slot of lower interconnecting piece, to drive upper bare terminal end close or separate lower bare terminal end, the adjusting
Component drives upper clamping part separate and close lower clamping part.
2. clamping device according to claim 1, it is characterised in that：The lower collet is arranged in parallel with upper grip.
3. clamping device according to claim 1, it is characterised in that：The adjusting part is adjusting screw rod, the upper folder
The traversing section of the upper interconnecting piece of head is equipped with screw hole up and down, and the lower collet corresponds to screwhole position and is equipped with the second through-hole, adjusts
The bottom end for saving screw rod is arranged in the second through-hole, and the top of adjusting screw rod passes through the screw hole of upper interconnecting piece, adjusting screw rod and upper company
Socket part is threadedly coupled, and drives upper grip to move up and down by turning adjusting screw rod, to drive the upper bare terminal end of upper grip close
It is clamped and release biomembrane with far from lower bare terminal end, realizing.
4. clamping device according to claim 1, it is characterised in that：The lower interconnecting piece of the lower collet is along its length
It sets to the concave to form sliding slot, the upper interconnecting piece of the upper grip is slided up and down along sliding slot.
5. clamping device according to claim 1, it is characterised in that：Further include limiting section, the lower collet is located at lower company
The position of socket part is provided along its length one section of slide opening, and the upper grip part for corresponding to slide opening position is equipped with through-hole, the limit
Position portion sequentially passes through through-hole and slide opening, and upper interconnecting piece is limited in the slide opening of lower interconnecting piece and slides, avoids by the limiting section
Interconnecting piece is detached from sliding slot, while the distance that upper interconnecting piece is slided is to limit the length of slide opening.
6. clamping device according to claim 2, it is characterised in that：The upper grip is inclined to set with lower collet, institute
The low side for stating the upper clamping part of upper grip is connect at an angle with upper interconnecting piece, the angle ranging from obtuse angle, the upper bare terminal end
It is arranged with resilient sleeve.
7. clamping device according to claim 2, it is characterised in that：The clamping device further includes limiting section, under described
The position that collet is located at lower interconnecting piece is provided along its length one section of slide opening, the length of the slide opening and the screw thread of adjusting screw rod
Segment length is identical, and the upper grip part for corresponding to slide opening position is equipped with through-hole, and the limiting section sequentially passes through through-hole and slide opening, institute
It states limiting section upper interconnecting piece is limited in the slide opening of lower interconnecting piece and slide, interconnecting piece is avoided to be detached from sliding slot, while by upper company
The distance of socket part sliding is to limit the length of slide opening.
8. a kind of cell device for precisely stretching, including shell further include the clamping device being set in shell for biomembrane to be clamped,
The clamping device includes at least two movable fixtures, and the movable fixture is for the folder described in any one of claim 1-7
Tight device, the biomembrane are located between two fixtures；The stretching device of clamping device movement is controlled in shell；The folder
The culture dish placement rack for culture dish holding is equipped between tight device.
9. cell device for precisely stretching according to claim 8, it is characterised in that：The cell device for precisely stretching includes
Cell culture apparatus, the cell culture apparatus provide CO2CO2Culture dish offer in conveying device, the cell culture apparatus is changed
The control system of the changing liquid automatically device and control stretching device stretching time, stretching frequency and stretch range of liquid function.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721344695.0U CN207918865U (en) | 2017-10-16 | 2017-10-16 | Clamping device for cell culture and the cell device for precisely stretching using the device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721344695.0U CN207918865U (en) | 2017-10-16 | 2017-10-16 | Clamping device for cell culture and the cell device for precisely stretching using the device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207918865U true CN207918865U (en) | 2018-09-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721344695.0U Expired - Fee Related CN207918865U (en) | 2017-10-16 | 2017-10-16 | Clamping device for cell culture and the cell device for precisely stretching using the device |
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| CN (1) | CN207918865U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114874878A (en) * | 2022-07-11 | 2022-08-09 | 浙江金仪盛世生物工程有限公司 | Support frame with two clamping states and biological reaction device |
-
2017
- 2017-10-16 CN CN201721344695.0U patent/CN207918865U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114874878A (en) * | 2022-07-11 | 2022-08-09 | 浙江金仪盛世生物工程有限公司 | Support frame with two clamping states and biological reaction device |
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Granted publication date: 20180928 |