CN110764243A

CN110764243A - Embryo observation device

Info

Publication number: CN110764243A
Application number: CN201911087738.5A
Authority: CN
Inventors: 云新
Original assignee: Wuhan Mutual Creation Joint Technology Co Ltd
Current assignee: Wuhan Mutual Creation Joint Technology Co Ltd
Priority date: 2019-11-08
Filing date: 2019-11-08
Publication date: 2020-02-07

Abstract

The invention discloses an embryo observation device, which comprises a workbench, a fixing clamp and a measuring mechanism, wherein the fixing clamp is arranged on the workbench, a through hole is formed in the fixing clamp and used for fixing a culture dish, the measuring mechanism comprises a driving component, a microscope and a light-emitting component, the microscope is positioned below the fixing clamp, the light-emitting component is positioned above the fixing clamp, a connecting line of the microscope and the light-emitting component vertically points to the through hole, the microscope is fixed on the driving component, the light-emitting component is fixed on the driving component, a moving end of the driving component drives the microscope and the light-emitting component to synchronously translate, and a telescopic end of the driving component drives the microscope to move towards a direction close to or far away from the light-emitting component; the problems that in the prior art, when large batches of embryos are continuously measured for a long time, time and labor are consumed, efficiency is low, and requirements are difficult to meet are solved.

Description

Embryo observation device

Technical Field

The invention relates to the technical field of embryo culture, in particular to an embryo observation device.

Background

At present, in the process of embryo culture, a microscope is needed to observe embryos so as to judge the growth conditions of the embryos.

Disclosure of Invention

The invention aims to overcome the technical defects and provide an embryo observation device, which solves the problems of time and labor consumption, low efficiency and difficulty in meeting the requirements when a large batch of embryos need to be continuously measured for a long time in the prior art.

In order to solve the technical problem, the invention provides an embryo observation device, which comprises a workbench, a fixed clamp and a measuring mechanism, the fixing clamp is arranged on the workbench, a through hole is arranged on the fixing clamp and used for fixing the culture dish, the measuring mechanism comprises a driving component, a microscope and a light-emitting component, the microscope is positioned below the fixing clamp, the light-emitting component is positioned above the fixing clamp, the connecting line of the microscope and the light-emitting component vertically points to the through hole, the microscope is fixed on the driving component, the light-emitting component is fixed on the driving component, the movable end of the driving component drives the microscope and the light-emitting component to synchronously translate, and the telescopic end of the driving component drives the microscope to move towards the direction close to or far away from the light-emitting component.

The invention has the beneficial effects that: different from the prior art, the invention has the advantages that the fixed clamp is arranged, a plurality of culture dishes can be placed on the fixed clamp at one time, specifically, the embryo is irradiated by the light-emitting component to provide a light source and is observed by the microscope, after an embryo is observed, the moving belt of the driving component can drive the microscope and the light-emitting component to synchronously translate on the workbench by arranging the driving component, the growth condition of the embryo in the next culture dish can be observed, time and labor are saved, in addition, the driving component is arranged, the telescopic end of the driving component can drive the microscope to move towards the direction close to or far away from the light-emitting component, the distance from the microscope to the light source can be adjusted, the focal length is adjusted, the observation of different layers of the embryo is carried out, the embryo does not need to be moved during the observation, and after one embryo is observed, the next embryo can be, observe convenient and fast.

Drawings

FIG. 1 is a schematic diagram of the construction of an embryo measurement device of the present invention;

FIG. 2 is a front view of the present invention;

fig. 3 is a side view of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

As shown in fig. 1, the present invention provides an embryo observation device, which includes a workbench 100, a fixing fixture (not shown) and a measuring mechanism 300, wherein in operation, the measuring mechanism 300 and the fixing fixture are both installed on the workbench 100, wherein the fixing fixture is fixed relative to the workbench 100, a plurality of culture dishes can be placed in the fixing fixture, specifically, the measuring mechanism 300 moves relative to the culture chamber 200, so that the measuring end of the measuring mechanism 300 is close to the culture dishes on the fixing fixture, after one of the culture dishes is observed, the next culture dish can be accurately found, which will be described in more detail below.

As shown in fig. 1, the working table 100 in this embodiment includes a base 110, a plurality of brackets 120 and a supporting plate 130, the base 110 and the supporting plate 130 are disposed in parallel, the plurality of brackets 120 are uniformly distributed around the base 110, each bracket 120 is in a T-shaped structure, one end of each bracket 120 is connected to the base 110, and the other end of each bracket 120 is connected to the supporting plate 130.

The base 110 and the support plate 130 are both flat plates, wherein the height of the support 120 is such that the microscope 320 can be adjusted in the vertical direction relative to the fixing jig, which facilitates the adjustment of the microscope 320, it is understood that the gap between the base 110 and the support is the size of the movable space of the microscope 320, and the shapes of the base 110 and the support plate 130 can be replaced by other structures.

The fixing clamp in this embodiment is installed on the workbench 100, and a through hole is formed in the fixing clamp, and the through hole is used for fixing the culture dish, it can be understood that the fixing clamp may be replaced by other mechanisms, and only a plurality of culture dishes may be fixed between the microscope 320 and the light emitting assembly 330.

Preferably, the fixing fixture is a culture chamber 200, the culture chamber 200 comprises a fixing frame 210 and a chamber body 220, the chamber body 220 is slidably connected with a chute formed in the side wall of the fixing frame 210, the chamber body 220 has a channel for gas inflow and outflow, gas with a mixed ratio of CO2 and O2 can be introduced, a constant pH value and an oxygen concentration can be maintained, and heating glass below the chamber body 220 can maintain a constant temperature of the culture chamber 220, so that the embryos can be guaranteed to survive for a long time.

More preferably, the number of the capsule bodies 220 may be multiple, the plurality of capsule bodies 220 correspond to the plurality of through holes formed in the side wall of the fixing frame 210 one to one, a plurality of culture dishes may be placed in one capsule body 220, and the capsule body 220 is provided with through holes for clamping the culture dishes.

Wherein, the cabin body 220 is the frame shape, and the opening of cabin body 220 top faces transparent glass, and transparent heating glass and temperature sensor are installed to the opening of cabin body 220 below, and the mounting hole has been seted up at the top of fixed frame 210, has placed transparent glass in the mounting hole, and the mounting hole faces the culture dish in the cabin body 220, and the bottom of fixed frame 210 has an opening, and the opening faces heating glass.

It can be understood that the fixing frame 210 and the chamber 220 are connected in a drawer type, the transparent glass installed above the fixing frame 210 faces the culture dish in the chamber 220, and when the light emitting assembly 330 emits light, the light emitting assembly 330 sequentially irradiates the transparent glass, the embryo in the culture dish, and the heat emitting glass until the light is irradiated into the microscope 320, thereby facilitating observation.

Furthermore, the culture compartment 200 can provide a desired growth environment for the embryo, and the embryo can be observed without moving the embryo after being cultured, which saves time and labor, so that the fixing clamp is the culture compartment 200, which is the most preferable scheme in this embodiment.

As shown in fig. 1, the measuring mechanism 300 in this embodiment includes a driving assembly 310, a microscope 320 and a light emitting assembly 330, the microscope 320 is located below the fixing fixture, the light emitting assembly 330 is located above the fixing fixture, a connecting line between the microscope 320 and the light emitting assembly 330 is vertically directed to the through hole, the microscope 320 is fixed on the driving assembly 310, the light emitting assembly 330 is fixed on the driving assembly 310, a moving end of the driving assembly 310 drives the microscope 320 and the light emitting assembly 330 to synchronously translate, and a telescopic end of the driving assembly 310 drives the microscope 320 to move toward or away from the light emitting assembly 330.

As shown in fig. 2-3, the driving assembly 310 includes a moving base 311, a lifting member 312, a first translating member 313 and a second translating member 314, one end of the lifting member 312 is connected to the moving base 311, the other end of the lifting member 312 is connected to the microscope 320, the lifting member 312 drives the microscope 320 to move up and down, the light emitting assembly 330 is fixed on the moving base 311 and located right above the microscope 320, one end of the first translating member 313 is connected to the moving base 311, an output end of the first translating member 313 drives the moving base 311 to move transversely, the other end of the first translating member 313 is connected to the second translating member 314, the second translating member 314 is installed on the base 110, an output end of the second translating member 314 drives the first translating member 313 to move transversely, and moving directions of the output end of the first translating member 313 and the output end of the second translating member 314 are perpendicular to each other.

Preferably, the lifting member 312 includes a motor, a lead screw, a fixed cylinder and a threaded pipe, the motor and the fixed cylinder are both mounted on the second translation member 314, an output shaft of the motor is connected with one end of the lead screw, the other end of the lead screw extends into the threaded pipe, an inner wall of the threaded pipe is in threaded connection with the lead screw, one end of the threaded pipe, which is far away from the motor, is connected with the microscope 320, and an outer wall of the threaded pipe is in sliding connection with an inner wall of the fixed cylinder.

Preferably, the first translating member 313 includes a first motor, a first guide rail and a first mounting block, the first motor and the first mounting block are both mounted on the first guide rail, the first guide rail is fixed on the second translating member 314, the telescopic end of the first motor is connected with the first mounting block, the first mounting block is slidably connected with the first guide rail, and the lifting member 312 is fixed on the first mounting block.

Preferably, the second translating element 314 includes a second motor, a second guide rail and a second mounting block, the second motor and the second mounting block are both mounted on the second guide rail, the second guide rail is fixed on the base 110, the telescopic end of the second motor is connected with the second mounting block, the second mounting block is slidably connected with the second guide rail, and the first translating element 313 is fixed on the second mounting block.

It can be understood that the lifting member 312, the first translating member 313 and the second translating member 314 constitute three output ends of the driving assembly 310 with three-axis linkage, specifically, the moving directions of the first translating member 313 and the second translating member 314 are perpendicular to each other, so as to drive the microscope 320 and the light emitting assembly 330 to translate to any position on the base 110, and the moving direction of the telescopic end of the lifting member 312 is perpendicular to the base 110, so as to drive the microscope 320 to move toward or away from the light emitting assembly 330.

Preferably, the microscope 320 is a phase contrast microscope, and the output end of the microscope 320 may be externally connected to a CCD.

Preferably, the light emitting assembly 330 includes a cold light LED lamp and a lamp cover, the cold light LED lamp is installed in the lamp cover, the lamp cover is fixed on the driving assembly 310, an opening direction of the lamp cover points to the fixing clamp, the influence of heat generated by light emission of the light emitting assembly 330 on embryo growth can be reduced by the cold light LED lamp, and the lamp cover can designate an illumination direction of the cold light LED lamp to avoid the influence on the surrounding environment.

The working process is as follows: clamping a plurality of culture dishes filled with embryos into a plurality of through holes on a fixing clamp, if the fixing clamp adopts a culture cabin 200, drawing a cabin body 220 out of a fixing frame 210, putting the culture dishes into the cabin body 220 in sequence, inserting the cabin body 220 into the fixing frame 210, starting a first motor and a second motor to drive a microscope 320 and a light-emitting component 330 to move relative to a base 110, enabling the microscope 320 and the light-emitting component 330 to simultaneously face one culture dish, starting the motors to drive a lead screw to rotate, connecting the lead screw with a threaded rod through threads, adjusting the microscope 320 connected with the threaded pipe to move up and down until the microscope 320 moves to a preset distance of the corresponding culture dish, starting the light-emitting component 330, sequentially irradiating transparent glass, embryos in the culture dishes and heating glass by the light-emitting component 330 until the transparent glass irradiates the microscope 320, facilitating observation, and continuously moving the microscope 320 up and down, and observing the growth conditions of different layers of the embryo, and repeating the steps after the observation is finished, so that the next culture dish can be observed.

Different from the prior art, the invention has the advantages that by arranging the fixing clamp, a plurality of culture dishes can be placed on the fixing clamp at one time, specifically, the embryo is irradiated by the light-emitting component 330 to provide a light source and is observed by the microscope 320, after an embryo is observed, the moving belt of the driving component 310 can drive the microscope 320 and the light-emitting component 330 to synchronously translate on the workbench 100 by arranging the driving component 310, the growth condition of the embryo in the next culture dish can be observed, time and labor are saved, in addition, by arranging the driving component 310, the telescopic end of the driving component 310 can drive the microscope 320 to move towards the direction close to or far away from the light-emitting component 330, the distance from the microscope 320 to the light source can be adjusted, the focal length is adjusted, the observation of different layers of the embryo is carried out, the embryo does not need to be moved when being observed, and after one embryo is observed, the next embryo can be, observe convenient and fast.

It should be noted that the above embodiments belong to the same inventive concept, and the description of each embodiment has a different emphasis, and reference may be made to the description in other embodiments where the description in individual embodiments is not detailed.

The above-mentioned embodiments only express the embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The utility model provides an embryo observation device, its characterized in that, includes workstation, mounting fixture and measuring mechanism, mounting fixture install in on the workstation, the through-hole has been seted up on the mounting fixture, the through-hole is used for the stationary culture dish, measuring mechanism includes drive assembly, microscope and light emitting component, the microscope is located mounting fixture's below, light emitting component is located mounting fixture's top, the microscope with light emitting component's line vertical orientation the through-hole, the microscope is fixed in on the drive assembly, light emitting component is fixed in on the drive assembly, drive assembly's removal end drives the microscope with light emitting component translation in step, drive assembly's flexible end drives the microscope is towards being close to or keeping away from the motion of light emitting component direction.

2. The embryo observation device according to claim 1, wherein the worktable comprises a base, a plurality of supports and a supporting plate, the base and the supporting plate are arranged in parallel, the plurality of supports are uniformly distributed around the base, each support is of a T-shaped structure, one end of each support is connected with the base, and the other end of each support is connected with the supporting plate.

3. The embryo observation device according to claim 1, wherein the fixture is a cultivation chamber, the cultivation chamber comprises a fixing frame and a chamber body, the chamber body is slidably connected with a sliding groove formed on a side wall of the fixing frame, and the chamber body has a gas inflow and outflow channel.

4. The embryo observation device according to claim 3, wherein the chamber body is shaped like a frame, the opening above the chamber body faces the transparent glass, the opening below the chamber body is provided with the transparent heating glass and the temperature sensor, the top of the fixing frame is provided with a mounting hole, the transparent glass is placed in the mounting hole, the through hole faces the culture dish in the chamber body, the bottom of the fixing frame is provided with an opening, and the opening faces the heating glass.

5. The embryo observation device according to claim 1, wherein the driving assembly comprises a moving base, a lifting member, a first translation member and a second translation member, one end of the lifting piece is connected with the movable seat, the other end of the lifting piece is connected with the microscope, the lifting piece drives the microscope to move up and down, the light-emitting component is fixed on the movable seat, and is positioned right above the microscope, one end of the first translation piece is connected with the moving seat, the output end of the first translation piece drives the movable seat to move transversely, the other end of the first translation piece is connected with the second translation piece, the second translation piece is arranged on the base, and the output end of the second translation piece drives the first translation piece to transversely move, and the motion directions of the output end of the first translation piece and the output end of the second translation piece are mutually vertical.

6. The embryo observation device according to claim 5, wherein the lifting member comprises a motor, a screw rod, a fixed cylinder and a threaded pipe, the motor and the fixed cylinder are both mounted on the second translation member, an output shaft of the motor is connected with one end of the screw rod, the other end of the screw rod extends into the threaded pipe, the inner wall of the threaded pipe is in threaded connection with the screw rod, one end of the threaded pipe, far away from the motor, is connected with the microscope, and the outer wall of the threaded pipe is in sliding connection with the inner wall of the fixed cylinder.

7. The embryo observation device according to claim 5, wherein the first translation member comprises a first motor, a first guide rail and a first mounting block, the first motor and the first mounting block are both mounted on the first guide rail, the first guide rail is fixed on the second translation member, the telescopic end of the first motor is connected with the first mounting block, the first mounting block is slidably connected with the first guide rail, and the lifting member is fixed on the first mounting block.

8. The embryo observation device according to claim 5, wherein the second translation member comprises a second motor, a second guide rail and a second mounting block, the second motor and the second mounting block are both mounted on the second guide rail, the second guide rail is fixed on the base, the telescopic end of the second motor is connected with the second mounting block, the second mounting block is slidably connected with the second guide rail, and the first translation member is fixed on the second mounting block.

9. An embryo observation device according to claim 1, wherein the microscope is a phase contrast microscope.

10. The embryo observation device according to claim 1, wherein the light emitting assembly comprises a cold light LED lamp and a lamp cover, the cold light LED lamp is mounted in the lamp cover, the lamp cover is fixed on the driving assembly, and an opening direction of the lamp cover points to the fixing clamp.