CN115381003A - Aquatic product antimicrobial-free fermented feed and preparation method thereof - Google Patents
Aquatic product antimicrobial-free fermented feed and preparation method thereof Download PDFInfo
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- CN115381003A CN115381003A CN202210807135.3A CN202210807135A CN115381003A CN 115381003 A CN115381003 A CN 115381003A CN 202210807135 A CN202210807135 A CN 202210807135A CN 115381003 A CN115381003 A CN 115381003A
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Images
Classifications
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- A—HUMAN NECESSITIES
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
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- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract
The invention relates to an aquatic product antimicrobial-free fermented feed and a preparation method thereof. The aquatic product antimicrobial-free fermented feed provided by the invention can enhance the capability of resisting oxidation and stress reaction of cultured animals by adding natural active ingredients and a novel animal and plant protein source, improve the immune function and muscle quality of the aquatic animals, contribute to enhancing the absorption and utilization efficiency of the aquatic animals to the protein source, reduce the feed coefficient and save the culture cost. In addition, the microbial fermentation technology adopted in the invention can effectively decompose anti-nutritional factors in the raw materials, can enhance the absorption conversion rate of the feed, promote the growth of the cultured animals and realize the healthy and sustainable development of aquaculture.
Description
Technical Field
The invention relates to the technical field of microbial fermented feed, in particular to an aquatic non-microbial fermented feed and a preparation method thereof.
Background
At present, china has become the largest aquaculture country in the world, the aquatic feed is the important material basis for the development of aquaculture, and the type and quality of the aquatic feed directly determine the economic benefit of the aquaculture industry. The problems of high breeding density, large feed input and residual amount, serious accumulation of aquatic animal excrement and the like generally exist in modern large-scale intensive breeding, and water quality pollution and wide-range epidemic diseases are often caused to reduce the production performance of the bred animals. In addition, with the unreasonable addition of antibiotics in aquatic feeds, phenomena such as drug residues and drug resistance frequently occur. Therefore, with the continuous transformation and upgrade of the aquaculture in China to the green and healthy aquaculture direction, the research and development of the environment-friendly functional high-activity aquaculture feed and the scientific and reasonable 'resistance' ecological aquaculture mode become the current hot spots.
The biological fermentation feed is a green and healthy product generally accepted nowadays, and has a plurality of advantages. The feed mainly takes a compound feed as a substrate, the raw materials of the feed are fermented and decomposed by adding compound microorganisms to generate beneficial products such as vitamins, digestive enzymes, polysaccharides, active small peptides, amino acids, immune enhancement factors, growth promotion factors and the like, and the adverse effects of anti-nutritional factors in the raw materials are eliminated, so that the palatability and the digestion utilization rate of the feed are improved, and the healthy growth of aquatic animals is promoted; the immune function of the cultured animals is increased, and the immunity-enhancing 'substitute antibody' effect is generated; improve the micro-ecological environment in the cultured animal body and improve the muscle quality of the cultured animal body. By using the biological fermentation feed, the bottleneck problems of low feed absorption rate, overproof drug residue, high water pollution, low aquatic animal quality and the like widely existing in the current aquaculture practice are solved, and the biological fermentation feed has important application value.
At present, the artificial compound feed is widely applied and has higher ratio in aquaculture. The feed is mainly prepared by mixing various raw materials according to a certain proportion and carrying out flow line production through industrial processing. The feed raw materials are rich in variety, and comprise plant raw materials, such as: soybean meal, rapeseed meal, cottonseed meal, wheat flour, peanut cake, and the like; also included are animal sources such as: fish meal, shrimp meal, bone meal, etc.; and also comprises feed additives such as yeast powder, mineral substances, phospholipid, calcium hydrophosphate and the like. Therefore, the artificial compound feed has the advantages of easy storage and transportation, balanced nutrition, various types and the like, plays the positive roles of water saving, emission reduction and the like in intensive culture, and is a feed mainly used in aquaculture.
The artificial compound feed inevitably has certain defects in the production process. Firstly, the vegetable protein source in the feed raw materials can generate various anti-nutritional factors in the actual production, which seriously influences the quality and utilization of the feed and reduces the nutritional value of the feed; even can generate antagonism to the absorption of nutrient substances in the intestinal tracts of animals, generate anaphylactic reaction and stress effect to immune tissues in organisms and influence the health and growth of the cultured animals. Secondly, fish meal is used most in animal raw materials, and the large-scale use of the fish meal can improve the production cost on one hand; on the other hand, the method can cause over-development of ocean resources, aggravate the shortage of production raw materials and influence the economic benefit of the aquaculture industry on the whole.
In addition, icy fresh bait is commonly used as main feed for aquaculture in the traditional mode, and the production method is to freeze and process caught fresh water and seawater wild small trash fish or juvenile fish of economic fish, so that the bait is palatable feed for carnivorous cultured animals. The frozen fresh bait does not pass through the processes of high temperature, high pressure, drying and the like in the production process, presents certain advantages, is rich in animal protein and grease, has the advantages of comprehensive nutritional value, rich essential amino acid and high digestion, absorption and utilization rate, and can accelerate the growth speed of aquatic animals; the frozen and fresh trash fish also has rich fatty acid and protein, and can improve the muscle quality and the nutritive value of the cultured animals. Thus, iced bait remains the preferred bait for many of today's pond and indoor small-scale farming of animals.
Practical studies have found that the icy fresh bait also presents a number of problems during use. Firstly, the iced fresh bait is not easy to store, and the manpower, material resources and cultivation cost are increased when the iced fresh bait is used; if the biological amine is improperly placed, a large amount of biological amine and pathogenic microorganisms are easily generated, so that the disease outbreak of the cultured animals is caused; secondly, the conversion efficiency of the iced fresh trash fish feed is low, and the feed coefficient is obviously increased; thirdly, the iced fresh feed is polluted greatly when in use, so that the water quality is easy to deteriorate, and the discharged wastewater can also destroy the ecological environment and influence the healthy development of aquaculture.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide an aquatic non-antimicrobial fermented feed. The fermented feed improves palatability and nutritive value, enhances the capability of resisting oxidation and stress reaction of cultured animals and promotes the growth performance of the cultured animals by adjusting the components and the proportion of the feed raw materials; the digestion and absorption efficiency of the aquatic animals to the feed can be improved by adding a new protein source; in addition, the anti-nutritional factors in the raw materials are effectively decomposed by means of a microbial fermentation technology, the utilization effect of the feed is enhanced, the immune function of cultured animals is improved, water pollution is reduced, the culture environment is improved, and the economic benefit of enterprises is improved.
In order to achieve the purpose, the invention adopts the technical scheme that:
an aquatic product antimicrobial-free fermented feed is characterized by comprising the following components in percentage by weight:
the feed comprises, by weight, 2-5 parts of mulberry leaf powder, 2-5 parts of spirulina powder, 12-20 parts of fermented soybean meal, 4-8 parts of fish meal, 5-9 parts of shrimp meal, 2-6 parts of cottonseed protein concentrate, 2-6 parts of soybean protein concentrate, 5-13 parts of tenebrio molitor powder, 13-24 parts of flour, 8-18 parts of bran, 0.5-3 parts of soybean oil, 0.5-3 parts of fish oil, 0.5-2 parts of soybean lecithin, 0.5-3.5 parts of aquatic product multivitamin, 0.1-1.5 parts of choline chloride, 1-4 parts of trace element premix, 0.5-3 parts of monocalcium phosphate, 0-1 part of salt and 2-6 parts of microbial leavening agent.
Further, the mass ratio of various activated strains in the microbial starter is as follows:
45% of bacillus subtilis, 10% of lactobacillus plantarum, 20% of saccharomyces cerevisiae and 25% of clostridium butyricum.
In addition, the microelement premix provides the following nutritional ingredients for each kilogram of feed: 5.0 mg of Cu, 80.0 mg of Zn, 100.0 mg of Fe100, 0.6 mg of Se, 2.0 mg of I, 2.0 mg of Co and 40.0 mg of Mn.
The invention also aims to provide a preparation method of the aquatic antimicrobial-free fermented feed.
In order to achieve the above purposes, the technical scheme adopted by the invention is as follows:
the preparation method of the aquatic product antimicrobial-free fermented feed is characterized by comprising the following steps:
step 1, preparing feed raw materials according to the content of the fermented feed components;
step 2, fully and uniformly mixing the microbial starter prepared in the step 1, flour, bran fish meal and fish meal shrimp meal, and pre-reacting for 1.5 hours;
and 3, continuously adding other fermented feed components prepared in the step 1 into the mixture obtained in the step 2, adjusting the water content to be 30%, and fermenting in a closed manner at the temperature of 30 ℃ for 48 hours to obtain the aquatic product antimicrobial-free fermented feed.
The invention relates to an aquatic product antimicrobial-free fermented feed and a preparation method thereof, and the aquatic product antimicrobial-free fermented feed has the beneficial effects that:
(1) The mulberry leaf powder and the spirulina powder added in the invention contain rich mineral substances, vitamins and natural active substance components, thus improving the palatability and the nutritional value of the feed and enhancing the capability of resisting oxidation and stress reaction of bred animals. Meanwhile, the immune function and muscle quality of aquatic animals can be improved, and the method plays a great role in healthy cultivation.
(2) The fermented soybean meal, the concentrated cottonseed protein, the concentrated soybean protein and the tenebrio molitor powder added in the invention belong to novel plant protein sources and animal protein sources, are low in anti-nutritional factors and rich in nutritional ingredients, improve the energy conversion efficiency of the feed, contribute to enhancing the absorption and utilization efficiency of aquatic animals on the protein sources, reduce the feed coefficient, save the breeding cost and improve the enterprise benefits.
(3) The microbial fermentation technology adopted in the invention can effectively decompose anti-nutritional factors in the raw materials, enhance the absorption conversion rate of the feed and promote the growth of the cultured animals; establishing the micro-ecological balance of the intestinal tracts of the cultured animals, and reducing the outbreak of digestive tract diseases; reduce water pollution, improve the culture environment and realize the healthy and sustainable development of aquaculture industry.
Drawings
The invention has the following drawings:
FIG. 1 is a technical flow diagram of the present invention.
Detailed Description
In order to further clarify the technical solutions and advantages of the present invention in detail, a scientific and complete description will be made below with reference to specific examples.
(1) Technical scheme
Example 1:
an aquatic product antimicrobial-free fermented feed comprises the following components in parts by weight: 5 parts of mulberry leaf powder, 4 parts of spirulina powder, 17 parts of fermented soybean meal, 5 parts of fish meal, 6 parts of shrimp meal, 4 parts of cottonseed protein concentrate, 4 parts of soybean protein concentrate, 11 parts of tenebrio molitor powder, 18 parts of flour, 10 parts of bran, 1 part of soybean oil, 2 parts of fish oil, 2 parts of soybean lecithin, 2 parts of aquatic product multivitamin, 1 part of choline chloride, 2 parts of trace element premix, 0.5 part of monocalcium phosphate, 0.5 part of salt and 5 parts of microbial starter culture.
The prepared microbial starter, flour, bran fish meal and fish meal shrimp meal are fully and uniformly mixed and pre-reacted for 1.5 hours.
And continuously adding other fermented feed components into the mixture, adjusting the water content to be 30%, and performing closed fermentation at 30 ℃ for 48 hours to obtain the aquatic product antimicrobial-free fermented feed.
Example 2:
an aquatic product antimicrobial-free fermented feed comprises the following components in parts by weight: 3 parts of mulberry leaf powder, 3 parts of spirulina powder, 16 parts of fermented soybean meal, 6 parts of fish meal, 7 parts of shrimp meal, 4 parts of cottonseed protein concentrate, 4 parts of soybean protein concentrate, 9 parts of tenebrio molitor powder, 19 parts of flour, 13 parts of bran, 1.5 parts of soybean oil, 1.5 parts of fish oil, 1 part of soybean lecithin, 2 parts of aquatic product multivitamin, 0.5 part of choline chloride, 3 parts of trace element premix, 1.5 parts of monocalcium phosphate, 0.5 part of salt and 4.5 parts of microbial starter.
The prepared microbial starter, flour, bran fish meal and fish meal shrimp meal are fully and uniformly mixed and pre-reacted for 1.5 hours.
And continuously adding other fermented feed components into the mixture, adjusting the water content to be 30%, and performing closed fermentation at 30 ℃ for 48 hours to obtain the aquatic product antimicrobial-free fermented feed.
Example 3:
an aquatic product antimicrobial-free fermented feed comprises the following components in parts by weight: 4 parts of mulberry leaf powder, 4 parts of spirulina powder, 18 parts of fermented soybean meal, 4 parts of fish meal, 5 parts of shrimp meal, 3 parts of cottonseed protein concentrate, 3 parts of soybean protein concentrate, 10 parts of tenebrio molitor powder, 21 parts of flour, 12 parts of bran, 1 part of soybean oil, 2 parts of fish oil, 1.5 parts of soybean lecithin, 2.5 parts of aquatic multivitamin, 1 part of choline chloride, 2.5 parts of trace element premix, 1 part of monocalcium phosphate, 0.5 part of salt and 4 parts of microbial starter.
The prepared microbial starter, flour, bran fish meal and fish meal shrimp meal are fully and uniformly mixed and pre-reacted for 1.5 hours.
Continuously adding other fermented feed components into the mixture, adjusting the water content to 30%, and fermenting in a sealed manner at 30 deg.C for 48 hr to obtain the final product.
Example 4:
an aquatic product antimicrobial-free fermented feed comprises the following components in parts by weight: 2 parts of mulberry leaf powder, 5 parts of spirulina powder, 12 parts of fermented soybean meal, 8 parts of fish meal, 9 parts of shrimp meal, 2 parts of cottonseed protein concentrate, 6 parts of soybean protein concentrate, 5 parts of tenebrio molitor powder, 24 parts of flour, 8 parts of bran, 0.5 part of soybean oil, 3 parts of fish oil, 0.5 part of soybean lecithin, 3.5 parts of aquatic product multivitamin, 0.1 part of choline chloride, 4 parts of trace element premix, 3 parts of monocalcium phosphate, 1 part of salt and 6 parts of microbial starter.
The prepared microbial starter, flour, bran fish meal and fish meal shrimp meal are fully and uniformly mixed and pre-reacted for 1.5 hours.
And continuously adding other fermented feed components into the mixture, adjusting the water content to be 30%, and performing closed fermentation at 30 ℃ for 48 hours to obtain the aquatic product antimicrobial-free fermented feed.
Example 5:
an aquatic product antimicrobial-free fermented feed comprises the following components in parts by weight: 3 parts of mulberry leaf powder, 2 parts of spirulina powder, 20 parts of fermented soybean meal, 7 parts of fish meal, 6 parts of shrimp meal, 6 parts of cottonseed protein concentrate, 2 parts of soybean protein concentrate, 13 parts of tenebrio molitor powder, 13 parts of flour, 18 parts of bran, 3 parts of soybean oil, 0.5 part of fish oil, 1 part of soybean lecithin, 0.5 part of aquatic product multivitamin, 1.5 parts of choline chloride, 1 part of trace element premix, 2.5 parts of monocalcium phosphate and 2 parts of microbial starter culture.
The prepared microbial starter, flour, bran fish meal and fish meal shrimp meal are fully and uniformly mixed and pre-reacted for 1.5 hours.
And continuously adding other fermented feed components into the mixture, adjusting the water content to be 30%, and performing closed fermentation at 30 ℃ for 48 hours to obtain the aquatic product antimicrobial-free fermented feed.
(2) Mass ratio of strain
The microbial starter is characterized in that the mass ratio of various activated strains in the microbial starter is as follows: 45% of bacillus subtilis, 10% of lactobacillus plantarum, 20% of saccharomyces cerevisiae and 25% of clostridium butyricum.
(3) Preparation method of aquatic product antimicrobial-free fermented feed
The preparation method of the aquatic antimicrobial-free fermented feed comprises the following steps:
(1) the inoculation amount of the microbial starter in the application is 2-6% of the total mass of the feed raw materials.
(2) Preparing the feed raw materials and the leavening agent according to the inoculation amount proportion, firstly, fully and uniformly mixing the microbial leavening agent with flour, bran, fish meal and shrimp meal in the feed raw materials in a closed fermentation barrel, and pre-reacting for 1.5 hours.
(3) Continuously adding the rest feed raw materials, adjusting the water content to be 30%, sealing and storing at 30 ℃ for fermentation for 48 hours to obtain the high-activity aquatic product antimicrobial-free fermented feed.
(4) Benefit analysis
The antimicrobial-free fermented feed for aquatic animals prepared in example 1, example 2 and example 3 (corresponding to experimental group 1, experimental group 2 and experimental group 3, hereinafter) was selected and the feeding effect thereof was examined. The common granulated feed purchased from the market is used as a control group, common crucian carps with the average body mass of 55.4 g/tail and the same growth environment are selected for testing, the feeding equipment is a glass culture water tank (1 m by 1.2 m), and 20 tails of the crucian carps are cultured in each water tank. The test period is 25 days, and the feeding process is implemented by feeding the feed once every morning and afternoon and sucking the residual feed by a siphon method. In order to ensure the accuracy of the data, the control group and the 3 experimental groups are repeated for three times, and the environmental conditions of all the water tanks are kept consistent in the test process. The fish body quality and the physiological index are measured before stocking and at the end of the experiment.
(1) Analysis of growth Performance
Growth performance in aquaculture industry is the most key index for evaluating economic benefit of cultured animals, in order to explore the influence of the aquatic antimicrobial-free fermented feed related to the invention on growth performance of crucian, the body mass and feed coefficient of each test group are determined and analyzed at the beginning and the end of the test, and the specific results are shown in table 1.
TABLE 1 growth Performance and feed coefficients of Carassius auratus under different feed sources
The data in table 1 show that the final body mass, average weight gain, and average weight gain of the three test groups after the end of the test are all higher than those of the control group, wherein the weight gain is most obvious in test group 1; meanwhile, the feed coefficients are all lower than those of the control group, and the test group 1 is the largest in reduction amplitude. Therefore, the aquatic product antimicrobial-free fermented feed can effectively improve the growth performance of the crucian and reduce the feed coefficient by feeding the feed, thereby saving the culture cost of enterprises and obviously improving the economic benefit.
(2) Analysis of immunological Properties
Important immune characteristic enzymes in the bodies of the aquatic animals mainly comprise superoxide dismutase, glutathione peroxidase, catalase, acid phosphatase, alkaline phosphatase, lysozyme, malondialdehyde, lactate dehydrogenase, glutamic-pyruvic transaminase and the like, and the immune performance of the aquatic animals can be indirectly reflected by measuring the activity of the related characteristic enzymes and by means of the change trend of the enzyme activity.
Table 2 activity of the enzyme for immune characteristics in crucian carp from different feed sources (data expressed as mean ± SD, n = 3)
TABLE 3 increase rate of enzyme for immune characteristics in Carassius auratus under different feed sources (data expressed as mean)
Based on the above principle, in order to explore the influence of different feed sources on the organism immunity of the crucian, the activity of the immune characteristic enzyme in the crucian in each test group is determined and analyzed at the end of the test, and the specific results are shown in table 2. In addition, the rate of change of the enzyme activity of each of the immune characteristics was further calculated, and the specific results are shown in Table 3. The data in tables 2 and 3 show that the activities of characteristic enzymes closely related to the body's antioxidant capacity and immunological capacity, including superoxide dismutase, glutathione peroxidase, catalase, acid phosphatase, alkaline phosphatase, lysozyme, malondialdehyde, were increased to various degrees, with the magnitude of the increase being most pronounced with malondialdehyde, followed by glutathione peroxidase and catalase. The data show that after the aquatic product antimicrobial-free fermented feed is fed, the oxidation and stress reaction resistance and the immune function of the cultured animals are obviously enhanced. Therefore, the feed plays a great role in improving the organism performance of the aquaculture animals and maintaining healthy aquaculture.
(3) Environmental benefit analysis
With the rapid development of aquaculture industry, china has become the largest aquaculture country in the world, and the aquaculture feed is an important material basis for aquaculture development and also an important factor for aquaculture water pollution. In recent years, the problems of drug residue, drug resistance and the like are caused along with the unreasonable addition of antibiotics in aquatic feeds, and a very serious food safety problem is brought. Therefore, the development of environment-friendly functional aquatic feeds has become a hot spot today. In order to examine the influence of the antimicrobial-free fermented feed for aquatic products designed in the invention on water quality, the pH, COD and ammonia Nitrogen (NH) in the culture water tanks of a control group and three test groups were measured every day during the test 3 N), total phosphorus (in P), total nitrogen content, the average data of each index are shown in Table 4.
Table 4 water quality analysis of the culture tanks for different feed sources (data expressed as mean ± SD, n = 3)
Based on the water quality indexes of the control group, the main pollutant analysis is carried out on the water quality conditions in other three test groups, and the specific data are shown in tables 5 and 6. The results show that indexes of COD, ammonia nitrogen, total phosphorus and total nitrogen in the water tanks of the three test groups are all lower than those of the control group, and the indexes show a descending trend. The most obvious reduction effect of four indexes of COD, ammonia nitrogen, total phosphorus and total nitrogen is test group 1. The change data of the pollutants show that the aquatic product antimicrobial-free fermented feed has the effects of reducing pollution and improving water quality after being fed, so that the occurrence probability and risk of diseases of cultured animals are reduced, and the culture benefit is improved.
TABLE 5 analysis of the variation of contaminants in the tanks of the test groups (data expressed as mean values based on the control group)
TABLE 6 reduction of contaminants in the tanks of the test groups (based on the control, data expressed as mean) for different sources of feed
With the large-scale development of aquaculture industry, the pollution of the water environment of the riverway around the freshwater aquaculture area is an important factor for causing the water pollution of the watershed. Aiming at developing water environment degradation and purifying water pollution in freshwater aquaculture areas, the concept of green and high-quality development of fishery is realized, the promotion of industrial transformation and upgrading is facilitated, the sustainable development is realized, and the ecological protection of 'two mountains' innovative bases is important. Based on this, we further evaluated the environmental benefits of the aquatic product after feeding the antimicrobial-free fermented feed according to the present invention.
The estimated reduction of pollutant emissions after feeding the antimicrobial-free fermented feed for aquatic products of the present invention was shown in Table 7, based on the change of various pollutants shown in tables 5 and 6, and the total water amount of 1 pond (10 mu of water surface, 1.8 m of water depth) in the large-scale outdoor culture area was 12006m 3. The results show that the test groups can greatly reduce the discharge amount of pollutants after implementation, effectively reduce the pollution load in rivers, and achieve the purposes of increasing carbon sink and reducing carbon emission, and the effect of the first test group is the most obvious in the three test groups. Therefore, compared with the common granulated feed in the market, the aquatic product antimicrobial-free fermented feed disclosed by the invention is beneficial to reducing water quality pollution, improving the culture environment and promoting healthy and sustainable development of aquaculture after being put into use.
TABLE 7 reduction of pollutant emissions for different feed sources (data expressed as mean based on control)
Those not described in detail in this specification are well within the skill of the art.
Claims (3)
1. An aquatic product antimicrobial-free fermented feed is characterized by comprising the following components in percentage by weight:
the feed comprises, by weight, 2-5 parts of mulberry leaf powder, 2-5 parts of spirulina powder, 12-20 parts of fermented soybean meal, 4-8 parts of fish meal, 5-9 parts of shrimp meal, 2-6 parts of cottonseed protein concentrate, 2-6 parts of soybean protein concentrate, 5-13 parts of tenebrio molitor powder, 13-24 parts of flour, 8-18 parts of bran, 0.5-3 parts of soybean oil, 0.5-3 parts of fish oil, 0.5-2 parts of soybean lecithin, 0.5-3.5 parts of aquatic multivitamin, 0.1-1.5 parts of choline chloride, 1-4 parts of trace element premix, 0.5-3 parts of monocalcium phosphate, 0-1 part of salt and 2-6 parts of microbial leavening agent.
2. The aquatic antimicrobial-free fermented feed according to claim 1, wherein the mass ratio of activated strains in the microbial starter is as follows:
45% of bacillus subtilis, 10% of lactobacillus plantarum, 20% of saccharomyces cerevisiae and 25% of clostridium butyricum.
3. A method for preparing the aquatic non-microbial fermented feed according to claim 1, comprising the steps of:
step 1, preparing feed raw materials according to the content of the fermented feed components described in claim 1 or 2;
step 2, fully and uniformly mixing the microbial starter prepared in the step 1, flour, bran fish meal and fish meal shrimp meal, and pre-reacting for 1.5 hours;
and 3, continuously adding other fermented feed components prepared in the step 1 into the mixture obtained in the step 2, adjusting the water content to be 30%, and fermenting in a closed manner at the temperature of 30 ℃ for 48 hours to obtain the aquatic product antimicrobial-free fermented feed.
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