CN115364028B

CN115364028B - Biological peptide activation repairing mask liquid and preparation method thereof

Info

Publication number: CN115364028B
Application number: CN202211137404.6A
Authority: CN
Inventors: 肖亚杰; 邹六均
Original assignee: Guangzhou La Doris Cosmetics Co ltd
Current assignee: Guangzhou La Doris Cosmetics Co ltd
Priority date: 2022-09-19
Filing date: 2022-09-19
Publication date: 2023-06-13
Anticipated expiration: 2042-09-19
Also published as: CN115364028A

Abstract

The application relates to a biological peptide activation repairing mask liquid and a preparation method thereof, wherein the mask liquid comprises the following raw materials: phase A: hyaluronic acid, sodium hyaluronate, glycerol polyether-26, carbomer, hydroxyethyl cellulose, butylene glycol, 1, 2-hexanediol, panthenol, emollient, disodium EDTA; and B phase: arginine; and C phase: an antioxidant; and D phase: purslane extract, beta-glucan, nicotinamide, wheat bran extract, a bio-peptide composition, a skin conditioner, a preservative and water. The preparation method comprises the following steps: s1, mixing the phase A with water, heating to 85 ℃, homogenizing and dispersing, and cooling to 50 ℃; s2, heating the phase C to 60 ℃, dissolving and transparentizing, and adding the phase A; s3, cooling to 45 ℃, putting the phase B and the phase D into the reactor while stirring, and uniformly stirring and dissolving; filtering and discharging to obtain the bioactive peptide activation repairing mask liquid. The biological peptide mask has the effects of improving the skin-activating and repairing effects of the biological peptide mask on the skin and enabling the functions of the biological peptide mask to be more comprehensive.

Description

Biological peptide activation repairing mask liquid and preparation method thereof

Technical Field

The application relates to the field of masks, in particular to a biological peptide activation repairing mask liquid and a preparation method thereof.

Background

The facial mask is a common skin care product for people. The types of the facial masks on the market are quite complicated, the effects are different, the biological peptide facial mask has very good caring effect on facial skin, and the biological peptide facial mask can inhibit cell deterioration, so that the loss of collagen in the skin is reduced, the skin relaxation state is improved, free radicals harmful to human bodies can be removed, the color spot breeding is reduced, and the biological peptide facial mask gradually becomes a new pet for consumer facial mask consumption.

However, in the process of using the bio-peptide mask, it is found that for users with symptoms such as redness, pimple, acne, itching, dryness and roughness caused by damaged skin horny layer of the face, the existing bio-peptide mask can only provide the most basic moisturizing and skin-brightening effects and cannot achieve good skin-activating repairing effects, so that the functions of the bio-peptide mask have larger limitations.

In view of the above problems, the inventors believe that existing biopeptid masks have the disadvantage of insufficient skin repair function.

Disclosure of Invention

In order to improve the skin-activating and repairing effects of the biological peptide mask on the skin and enable the functions of the biological peptide mask to be more comprehensive, the application provides a biological peptide-activating and repairing mask liquid and a preparation method thereof.

In a first aspect, the present application provides a bio-peptide activation repair mask solution, which adopts the following technical scheme:

a biological peptide activation repairing mask liquid comprises the following raw materials in percentage by mass:

phase A: 0.03-0.07% of hyaluronic acid, 0.02-0.04% of sodium hyaluronate, 0.8-2.2% of glycereth, 0.10-0.14% of carbomer, 0.01-0.03% of hydroxyethyl cellulose, 3.8-4.2% of butanediol, 0.013-0.017% of 1, 2-hexanediol, 0.3-0.7% of panthenol, 0.2-0.4% of emollient and 0.01-0.03% of EDTA disodium;

and B phase: arginine 0.08-0.1%;

and C phase: 2.0 to 2.4 percent of antioxidant;

and D phase: 0.17 to 0.21 percent of purslane extract, 0.004 to 0.006 percent of beta-glucan, 0.2 to 0.4 percent of nicotinamide, 0.08 to 0.1 percent of wheat bran extract, 0.25 to 0.35 percent of biological peptide composition, 0.8 to 1.2 percent of skin conditioner and 0.55 to 0.65 percent of preservative;

the balance of water.

According to the application, the synergistic combination of the sodium hyaluronate and the hyaluronic acid and the thickening adjustment of carbomer, glycereth-26 and hydroxyethyl cellulose enable the mask liquid to form a long-acting protective film on the surface of skin to lock skin moisture; by adding beta glucan, wheat bran extract, purslane extract, nicotinamide, biological peptide composition, skin conditioner, emollient and other components, the active ingredients in the plant extract and polysaccharide, protein and the like are mutually matched, so that the facial mask liquid has a good transdermal absorption effect, nutrient components in the facial mask liquid can quickly and fully permeate the skin bottom layer, the skin basal layer is helped to establish a water storage layer, abundant skin basal layer moisture is promoted to move to the skin surface, and the moisture is promoted to flow from a moisture-rich region to a moisture-deficient region, so that skin water circulation is realized, and further the long-term moisturizing effect is achieved; stimulating the generation of collagen in skin, scavenging free radicals, and realizing the skin activating and whitening effects; furthermore, the components interact, so that metabolism of skin epidermal cells can be promoted, a stratum corneum is repaired, bacteria such as propionibacterium acnes are inhibited, damaged cells on the face are repaired, the problems of facial acne and redness, dry skin and dark skin color are solved, the improvement of skin resistance is promoted, the tolerance of the skin is improved, and the effects of moisturizing, shining and uniform skin color of the skin are achieved from inside to outside.

Particularly, when the skin is subjected to skin activation by the mask liquid, and then water emulsion maintenance is carried out, the absorption capacity of the skin to the water emulsion is greatly improved, so that the effect of daily skin maintenance is enhanced, and the mask liquid prepared by the mask liquid is good in functionality and practicality.

Preferably, the hyaluronic acid comprises small molecular hyaluronic acid of 4-6000 daltons and macromolecular hyaluronic acid of 100-130W daltons, and the mass ratio of the small molecular hyaluronic acid to the macromolecular hyaluronic acid is 2:1.

Through adopting above-mentioned technical scheme, small molecule hyaluronic acid helps promoting skin to absorb the active material, and macromolecular hyaluronic acid then can stay in the skin top layer, with sodium hyaluronate and other moisturizing ingredient coaction, maintains skin external barrier, and then pinning skin moisture for the skin can be moisturized for a long time. The matching of the large molecule and the small molecule hyaluronic acid is more beneficial to improving the skin tendering and repairing effects of the mask liquid on the skin.

Preferably, the skin conditioning agent consists of hydrolyzed sweet almond protein 23-27% wt, yeast 2.3-2.7% wt, ethylhexyl glycerin 0.08-0.12% wt, and the balance of solvent.

By adopting the technical scheme, hydrolyzed sweet almond protein not only contains a plurality of amino acids, but also has higher lipoprotein content and good lipoprotein affinity, and can effectively moisten and condition skin; the yeast contains rich amino acids, polypeptides and other nutrient substances, is easy to be absorbed by skin, can well help skin to moisturize and lock water, can also play a role in repairing skin barrier to a certain extent, improves bad skin state, and has good functions of resisting aging, resisting oxidation, whitening and the like when a small amount of vitamins in the yeast are used on the skin. According to the skin conditioner prepared by mixing and dissolving hydrolyzed sweet tonsil protein, yeast and ethylhexyl glycerin, a skin channel can be opened, other active ingredients in the mask liquid can permeate into the inner layer of the skin more easily, proliferation of collagen and the like in the skin is promoted, and further activity of skin cells is improved, so that an effect of activating and conditioning the inside of the skin is achieved.

Preferably, the emollient comprises rhodophyta extract, sodium (ester) copolymer of acrylic acid and bis-PEG-15 methyl ether polydimethoxyane, and the mass ratio of rhodophyta extract, sodium (ester) copolymer of acrylic acid and bis-PEG-15 methyl ether polydimethoxyane is 1:4:10.

By adopting the technical scheme, the rhodophyta extract, the acrylic acid (ester) copolymer sodium and the bis-PEG-15 methyl ether polydimethylsiloxane are matched for use, so that the required moisture can be timely supplemented for the dry skin cuticle, the skin is strong in moisturizing and astringing performance, the excessive secretion sebum of oily skin can be improved, capillary shrinkage, skin fineness and skin softness and moisture are promoted; the deep water supplementing and puffing functions of the rhodophyta extract can enable the damaged or sunken skin to recover the firmness and elasticity after the skin is fully supplemented with water.

Preferably, the biopeptides composition includes decapeptides-40.011%wt, glycoprotein 0.011%wt, disodium hydrogen phosphate 0.15-0.22%wt, sodium chloride 0.8-1.2%wt, and water balance.

By adopting the technical scheme, the decapeptide can penetrate the dermis to increase collagen, reverse the skin aging process by rebuilding from inside to outside, stimulate collagen and elastic fiber proliferation, improve the water content and water locking degree of skin, and increase the thickness of skin barrier; the glycoprotein has the functions of improving skin water retention and moistening; on one hand, the biological peptide composition can relieve the stimulation of antibacterial and anti-inflammatory components in plant extracts to skin, increase the generation of intercellular mucilage of the skin and smooth acne pits; on the other hand, the skin water circulation construction can be promoted together with other components of the mask liquid, so that the skin is soft, moist and bright from inside to outside.

Preferably, the preservative consists of 5-7% wt of octanoyl hydroxamic acid, 2-4% wt of ethylhexyl glycerol and the balance propylene glycol; the antioxidant comprises 1, 3-propanediol and p-hydroxyacetophenone, and the mass ratio of the 1, 3-propanediol to the p-hydroxyacetophenone is 10:1.

Through adopting above-mentioned technical scheme, can add components such as vitamin C, citric acid in the current skin brightening facial mask, these components are easy oxidation and are discoloured, not only be unfavorable for the storage stability of product, but also seriously can lead to the person of taking turns to the allergy phenomenon, this application adopts octanoyl hydroxamic acid, ethylhexyl glycerol and propylene glycol as antiseptic, 1, 3-propylene glycol and p-hydroxyacetophenone as the antioxidant, the utility of this application anticorrosive system internal component is mild, oxidation resistance is stronger, and have certain moisturizing effect, and then be favorable to the production sales of this application preparation facial mask liquid, can also strengthen the moisturizing efficiency of facial mask liquid.

In a second aspect, the preparation method of the bioactive peptide activation repair mask liquid provided by the application adopts the following technical scheme:

a preparation method of a biological peptide activation repairing mask liquid comprises the following steps:

s1, mixing the phase A raw material and water, putting into an emulsifying pot, heating to 85 ℃, homogenizing, dispersing, uniformly stirring, and cooling to 50 ℃; s2, independently heating the C-phase raw material to 60 ℃, dissolving and transparentizing, and adding the C-phase raw material into an emulsifying pot;

s3, cooling to 45 ℃, putting the B phase and D phase raw materials into an emulsifying pot while stirring, and uniformly stirring and dissolving; filtering and discharging to obtain the bioactive peptide activation repairing mask liquid.

By adopting the technical scheme, the solvent moisturizing component in the A-phase raw material is firstly subjected to homogeneous dispersion to obtain the basic mask liquid, and then the independently treated antioxidant, the pH regulator, the plant extract in the D-phase raw material, the biological peptide and other active components are added, so that the prepared mask liquid is uniform in component and stable in performance, and has multiple effects of promoting skin water circulation, tendering and whitening, and repairing skin barriers.

In summary, the present application includes at least one of the following beneficial technical effects:

1. the mask liquid prepared by the application is added with a plurality of components, wherein the synergistic combination of sodium hyaluronate and hyaluronic acid and the thickening adjustment of carbomer, glycereth-26 and hydroxyethyl cellulose enable the mask liquid to form a long-acting protective film on the surface of skin and lock skin moisture; the active ingredients of the plant extracts, polysaccharide and protein in the components of beta glucan, wheat bran extract, purslane extract, nicotinamide, biological peptide composition, skin conditioner, emollient and the like are mutually matched, so that the facial mask liquid has a good transdermal absorption effect, and nutrient components in the facial mask liquid can quickly and fully permeate the bottom layer of skin, realize skin water circulation from the basal layer of skin to the epidermis, and further achieve the effect of long-term moisturizing; the active ingredients extending into the basal layer of the skin can stimulate the generation of collagen, remove free radicals and realize the skin activating and whitening effects of the skin; the components cooperate with each other to realize double coordination between the inside and the outside of the skin, which is beneficial to promoting metabolism of skin epidermal cells, repairing the stratum corneum, promoting improvement of skin resistance, promoting skin tolerance, and achieving the effects of moisturizing and brightening the skin and homogenizing skin color from inside to outside; particularly, the skin-activating effect of the mask liquid on the skin enables the absorption capacity of the skin on the water emulsion to be greatly improved when the water emulsion is maintained after the mask liquid is used, so that the daily skin maintenance effect is enhanced, and the mask liquid prepared by the method is good in functionality and practicality; 2. the hyaluronic acid comprises 4-6000 daltons of small-molecule hyaluronic acid and 100-130W daltons of large-molecule hyaluronic acid, the small-molecule hyaluronic acid is helpful for promoting the absorption of active substances by skin, the large-molecule hyaluronic acid can stay on the surface layer of the skin, and the large-molecule hyaluronic acid acts together with sodium hyaluronate and other moisturizing components to maintain the barrier of the outer layer of the skin; the matching of the macromolecule and the micromolecular hyaluronic acid establishes a good channel for the internal and external water circulation of the skin, is more beneficial to improving the moisturizing and repairing effects of the mask liquid on the skin, and achieves the effects of long-term moisturizing and skin tendering;

3. the skin conditioner comprises hydrolyzed sweet tonsil protein, yeast, ethylhexyl glycerol and the balance of solvent, and the skin conditioner with special configuration can help to open a skin channel, so that other active ingredients in the mask liquid can more easily permeate into the inner layer of the skin, promote proliferation of collagen and the like in the skin, further promote activity of skin cells, and realize the effect of activating and conditioning the inside of the skin.

Detailed Description

Preparation example

Preparation example 1

The preparation example discloses a preparation method of an emollient, which comprises the following specific steps: adding 0.7kg of rhodophyta extract, 2.7kg of acrylic acid (ester) copolymer sodium and 6.6 bis-PEG-15 methyl ether polydimethylsiloxane into an emulsifying pot, mixing, and stirring at normal temperature and rotating speed of 100r/min for 3min to obtain the emollient.

Preparation example 2

The preparation example discloses a preparation method of an emollient, which comprises the following specific steps: 8kg of lactobacillus/rice fermentation product, 1.5kg of sweet almond oil and 0.5kg of 1, 2-pentanediol are added into an emulsifying pot to be mixed, and stirred for 5min at the normal temperature and the rotating speed of 45r/min, thus obtaining the emollient.

Preparation example 3

The preparation example discloses a preparation method of a biological peptide composition, which comprises the following steps: 1.1g of decapeptide-4, 1.1g of glycoprotein, 16g of disodium hydrogen phosphate, 80g of sodium chloride and 9901.8g of water are added into an emulsifying pot to be mixed, and stirred for 5min at the normal temperature and the rotating speed of 30r/min, so as to obtain the biological peptide composition.

Preparation example 4

The preparation example discloses a preparation method of a biological peptide composition, which comprises the following steps: 1.1g of decapeptide-4, 1.1g of glycoprotein, 20g of disodium hydrogen phosphate, 120g of sodium chloride and 9857.8g of water are added into an emulsifying pot to be mixed, and stirred for 5min at the normal temperature and the rotating speed of 30r/min, so as to obtain the biological peptide composition.

Preparation example 5

The preparation example discloses a preparation method of a biological peptide composition, which comprises the following steps: 1.1g of decapeptide-4, 1.1g of glycoprotein, 18g of disodium hydrogen phosphate, 100g of sodium chloride and 9879.8g of water are added into an emulsifying pot to be mixed, and stirred for 5min at the normal temperature and the rotating speed of 30r/min, so as to obtain the biological peptide composition.

Preparation example 6

The preparation example discloses a preparation method of a skin conditioner, which comprises the following specific steps: 2.3kg of hydrolyzed sweet tonsil protein, 0.23kg of yeast, 0.008kg of ethylhexyl glycerol, 0.1kg of 1, 2-hexanediol and 7.362kg of water are added into an emulsifying pot and mixed, and stirred for 10min at the normal temperature and the rotating speed of 30r/min, thus obtaining the skin conditioner.

Preparation example 7

The preparation example discloses a preparation method of a skin conditioner, which comprises the following specific steps: 2.7kg of hydrolyzed sweet tonsil protein, 0.27kg of yeast, 0.012kg of ethylhexyl glycerol, 0.1kg of 1, 2-hexanediol and 6.918kg of water are added into an emulsifying pot and mixed, and stirred for 10min at the normal temperature and the rotating speed of 30r/min, thus obtaining the skin conditioner.

Preparation example 8

The preparation example discloses a preparation method of a skin conditioner, which comprises the following specific steps: 2.5kg of hydrolyzed sweet tonsil protein, 0.25kg of yeast, 0.01kg of ethylhexyl glycerol, 0.1kg of 1, 2-hexanediol and 7.14kg of water are added into an emulsifying pot and mixed, and stirred for 10min at the normal temperature and the rotating speed of 30r/min, so as to obtain the skin conditioner.

Preparation example 9

The preparation example discloses a preparation method of a skin conditioner, which comprises the following specific steps: 9.8kg of lactobacillus/rice fermentation product, 0.1kg of 1, 2-pentanediol and 0.1kg of 1, 2-hexanediol are added into an emulsifying pot to be mixed, and the mixture is stirred for 5min at the normal temperature and the rotating speed of 35r/min, thus obtaining the skin conditioning agent.

Preparation example 10

The preparation example discloses a preparation method of a preservative, which comprises the following steps: 0.5kg of octanoyl hydroxamic acid, 0.2kg of ethylhexyl glycerol and 9.3kg of propylene glycol are added into an emulsifying pot to be mixed, and stirred for 5min at the normal temperature and the rotating speed of 30r/min, thus obtaining the preservative.

PREPARATION EXAMPLE 11

The preparation example discloses a preparation method of a preservative, which comprises the following steps: 0.7kg of octanoyl hydroxamic acid, 0.4kg of ethylhexyl glycerol and 8.9kg of propylene glycol are added into an emulsifying pot to be mixed, and stirred for 5min at the normal temperature and the rotating speed of 30r/min, thus obtaining the preservative.

Preparation example 12

The preparation example discloses a preparation method of a preservative, which comprises the following steps: 0.6kg of octanoyl hydroxamic acid, 0.3kg of ethylhexyl glycerol and 9.1kg of propylene glycol are added into an emulsifying pot to be mixed, and stirred for 5min at the normal temperature and the rotating speed of 30r/min, thus obtaining the preservative.

Examples

Examples 1 to 5

The embodiments 1-5 disclose a biological peptide activation repairing mask liquid, which comprises the following raw materials in percentage by mass:

and B phase: arginine 0.08-0.1%;

and C phase: 2.0 to 2.4 percent of antioxidant;

and D phase: 0.15 to 0.20 percent of purslane extract, 0.004 to 0.006 percent of beta-glucan, 0.2 to 0.4 percent of nicotinamide, 0.08 to 0.1 percent of wheat bran extract, 0.25 to 0.35 percent of biological peptide composition, 0.8 to 1.2 percent of skin conditioner and 0.55 to 0.65 percent of preservative;

the balance of water;

wherein the hyaluronic acid comprises small molecular hyaluronic acid of 4-6000 daltons and macromolecular hyaluronic acid of 100W-130W daltons, and the mass ratio of the small molecular hyaluronic acid to the macromolecular hyaluronic acid is 2:1; the skin conditioner consists of 23-27% wt of hydrolyzed sweet tonsil protein, 2.3-2.7% wt of yeast, 0.08-0.12% wt of ethylhexyl glycerol and the balance of solvent; the emollient comprises rhodophyta extract, acrylic acid (ester) copolymer sodium and bis-PEG-15 methyl ether polydimethoxyane, and the mass ratio of the rhodophyta extract, the acrylic acid (ester) copolymer sodium and the bis-PEG-15 methyl ether polydimethoxyane is 1:4:10; the biological peptide composition comprises decapeptide-40.011%wt, glycoprotein 0.011%wt, disodium hydrogen phosphate 0.15-0.22%wt, sodium chloride 0.8-1.2%wt and water balance; the preservative consists of 5-7% wt of octanoyl hydroxamic acid, 2-4% wt of ethylhexyl glycerol and the balance of propylene glycol; the antioxidant comprises 1, 3-propanediol and p-hydroxyacetophenone, and the mass ratio of the 1, 3-propanediol to the p-hydroxyacetophenone is 10:1.

Examples 1-5 also disclose the preparation method of the above-described bio-peptide activation repair mask liquid:

s1, mixing the phase A raw material and water, putting into an emulsifying pot, heating to 85 ℃, homogenizing for 3min under the condition of 800r/min to completely dissolve the phase A raw material and water without particles, and cooling to 50 ℃;

s2, adding the C-phase raw material into a heating pot, independently heating to 60 ℃, dissolving until the C-phase raw material is transparent, and then adding the C-phase raw material into an emulsifying pot;

s3, cooling to 45 ℃, mixing the B phase and D phase raw materials in an emulsifying pot while stirring, and stirring for 15min under the condition of the rotating speed of 40 r/min; filtering and discharging by a 200-mesh filter screen to obtain the bioactive peptide activation repairing mask liquid.

The raw material components and the amounts (unit: kg) of the additives of examples 1 to 5 are shown in Table 1.

TABLE 1

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Comparative example

Comparative example 1

The preparation method of the bioactive peptide-activated repairing mask liquid is different from that of the embodiment 1 in that: the purslane extract was replaced with an equal amount of water.

Comparative example 2

The preparation method of the bioactive peptide-activated repairing mask liquid is different from that of the embodiment 1 in that: the beta-glucan was replaced with an equal amount of water.

Comparative example 3

The preparation method of the bioactive peptide-activated repairing mask liquid is different from that of the embodiment 1 in that: nicotinamide was replaced with an equal amount of water.

Comparative example 4

The preparation method of the bioactive peptide-activated repairing mask liquid is different from that of the embodiment 1 in that: the wheat bran extract was replaced with an equal amount of water.

Comparative example 5

The preparation method of the bioactive peptide-activated repairing mask liquid is different from that of the embodiment 5 in that: the skin conditioning agent is replaced with an equal amount of water.

Performance test

The non-woven fabric mask paper with the absorptivity of 95% and the same regulation is respectively soaked in the mask liquid prepared in the examples 1-5 and the comparative examples 1-5, and is taken out after 24 hours to serve as a test example, a commercial biological peptide mask with a certain brand is taken as a comparative example, and the moisture content of the mask in the test example and the moisture content in the comparative example are consistent within an allowable error range (+ -1%).

Test 1, propionibacterium acnes antibacterial detection: referring to GB15979-2002 'Disposable sanitary articles sanitary Standard' appendix C4 'dissolution antibacterial product antibacterial property test method', mask liquids of examples 1-5 and comparative examples are diluted into 2% diluent respectively; washing a culture of propionibacterium acnes for 24 hours with 0.03mol/L phosphate buffer solution to prepare 104-106CFU/mL bacterial suspension; 1g of test sample is respectively taken, 0.1mL of the bacterial suspension is added for uniform coating and mixing, timing is started, 9mL of 0.03mol/L phosphate buffer solution is added after 20min for full mixing, after proper dilution is carried out, 3 dilutions are taken, 1mL of the solution is respectively taken and placed in two parallel plates, and then the corresponding culture medium is added for culturing for 24 hours under the anaerobic condition at 36+/-1 ℃ for colony counting. Repeating the above test for 3 times, taking average, and setting blank control group; and calculating the bacteriostasis rate. The calculation formula of the bacteriostasis rate is as follows: the bacteriostasis rate X= (A-B)/A multiplied by 100 percent, wherein A is average recovered colony of blank control sample, and B is average recovered colony of test sample; the specific results are shown in Table 2.

Test 2, test for relief of facial redness: selecting 55 testers with the condition of redness of the face, wherein the ages of the testers are 18-45 years, one group of 5 testers is selected for men and women, the testers are divided into 11 groups, each group is respectively coated with the mask of the embodiment 1-5, the comparative embodiment 1-5 and the comparative embodiment at about 10 pm every day for 15 minutes, and the embodiment 1-5, the comparative embodiment 1-5 and the comparative embodiment mask are respectively scored according to the condition of red blood streak of the skin and the use feeling after one week of use, the specific results are shown in the table 2, and the evaluation criteria are as follows:

1 part-after one week of use, the redness degree of the face does not decline, and the effect is basically not achieved;

2 minutes-the redness degree of the face is reduced after one week of use, but the effect of the reduction is not obvious;

3 minutes-the redness degree of the face is reduced after one week of use, and the reduction effect is obvious;

4 minutes-the redness degree of the face is reduced after three days of use, and the reduction effect is obvious;

5 minutes-the redness of the face is reduced in the next day, and the reduction effect is obvious.

Test 3, long-acting moisturizing effect test: selecting 110 testers, respectively applying the masks of examples 1-5, comparative examples 1-5 and comparative examples for 15 minutes for each 10 testers, taking down the mask, cleaning the residual mask liquid on the face, and measuring the moisture content of the skin horny layer at the forehead of each tester group by adopting a Callgari Soft5.5 skin horny layer moisture content tester, and marking the moisture content as a basic value; under the conditions of room temperature and 75-80% humidity, after 30, 60 and 120 minutes of indoor waiting, the water content of the skin horny layer of the face of the test group tester is measured, and the skin water content increment (the water content is positive when the water content is increased compared with a basic value and negative when the water content is decreased compared with the basic value) is calculated for 30, 60 and 120 minutes; data are recorded and the results averaged for each panel, with specific results shown in Table 2.

Test 4, skin rejuvenation effect test: the skin elasticity change rates (%) of the skin elasticity before and after the skin elasticity tester Corneometer MPA580 was used to test the skin elasticity of the testers before and after one month of the facial mask were calculated and recorded using the example 1-5, comparative example 1-5 and control group facial masks after cleaning the face every monday, three, five, and daily, respectively. The rate of change of skin elasticity is calculated by: the change rate (%) = [ (skin elasticity after mask application-skin elasticity before mask application)/skin elasticity before mask application ] ×100% and the average value of each group of test results is obtained, the larger the change rate value is, the better the skin elasticity is, and the specific results are shown in table 2.

Test 5, DPPH-free radical scavenging test: the mask solutions prepared in examples 1 to 5 and comparative examples 1 to 5 and the mask solution of comparative example were diluted with deionized water to prepare test solutions having a mass fraction of 1%. Taking 4mL of DPPH-absolute ethanol solution with the concentration of 0.1mmol/L, adding 2mL of test sample into a container, uniformly mixing, keeping out of the sun for 30min at room temperature, measuring absorbance at 517nm wavelength, and recording the absorbance value as C1; taking 4mL of absolute ethanol solution and 2mL of test sample solution, and recording the absorbance value as C2 under the same conditions; the absorbance was measured under the same conditions as above using a 4mL dry ethanol solution of 4mL PPH and a 2mL dry ethanol solution and was recorded as C3. Calculating the DPPH free radical clearance: DPPH free radical clearance (%) = [1- (C1-C2)/C3 ]. Times.100%, taking the average value of three measurements, the greater the clearance, the stronger the antioxidant and anti-aging capabilities, and the specific results are shown in Table 2.

Test 6, skin transdermal absorption test:

(1) Preparation of mouse skin

After the mice are killed by breaking the necks, the abdomen Mao Ti of the mice are immediately cleaned by a rechargeable trimmer, the abdomen skin is immediately sheared, the removed skin is flatly laid on a clean glass plate, the stratum corneum is downward, subcutaneous fat layers and connective tissues are carefully erased by cotton balls stained with physiological saline, repeatedly washed clean by the physiological saline, placed in the physiological saline, preserved in a 4C refrigerator for standby, naturally thawed before the experiment, soaked in the physiological saline for 0.5h, and the experiment is carried out within 1 week. The integrity of the mouse skin was visually checked before each experiment and there could not be any breakage. Preparing 6 mouse skins, and dividing the mouse skins into an experimental group and a control group, wherein each group comprises three mouse skins; respectively marked as experimental groups 1-1, 1-2, 1-3, and control groups 2-1, 2-2, 2-3;

(2) The mask of example 3 and the mask of the comparative example are respectively applied on three mouse skins of each group, the mouse skins are taken down after 15min, and residual mask liquid on the mouse skins is washed clean by deionized water, so as to obtain the mouse skins after the mask is applied;

(3) Franz diffusion cell for in vitro transdermal absorption experiments

A suitable amount of water was added to the thermostatic bath of the in vitro permeation diffusion device. The power supply and the constant temperature tank are started to magnetically stir, and the water temperature in the constant temperature tank is set to be 37+/-0.1 ℃. Fixing the mouse skin prepared in the step (2) between two diffusion cells by using an iron clamp, adding 5mL of receiving liquid (namely sodium chloride solution with the mass concentration of 0.9%) into a receiving cell of a vertical diffusion cell, placing the receiving cell into a constant temperature tank of an external permeation diffusion device for preheating, and setting the stirring speed of the receiving cell to be 300r/min. The method comprises the steps of slowly adding sample solution into a supply tank by using an injector, uniformly selecting an emulsion of a brand A as the sample solution in an experiment group 1-1 and a control group 2-1, uniformly selecting an emulsion of a brand B as the sample solution in the experiment group 1-2 and the control group 2-2, uniformly selecting an emulsion of a brand C as the sample solution in the experiment group 1-3 and the control group 2-3, wherein the sample solution contains sodium hyaluronate components, the adding amount of the sample solution is 2mL, and the upper opening is sealed by a preservative film. At the beginning, when the sample permeates 0, 2, 4, 6, 8 and 10 hours, 2mL of the receiving liquid is respectively taken in a centrifuge tube, the receiving liquid is supplemented into the receiving tank and bubbles in the tank are removed at the same time of sampling each time, the content of sodium hyaluronate in the receiving liquid is measured, and the accumulated permeation quantity Q (mg/cm) is calculated according to a formula ² ). The calculation formula is as follows:

the bottom diameter of the diffusion cell for test is 1.50cm, and the contact area of the sample is 1.60cm ² The method comprises the steps of carrying out a first treatment on the surface of the Q is the accumulated permeation quantity, S is the transdermal diffusion area, V is the volume of the receiving chamber of the Franz diffusion cell, cn is the concentration of the receiving liquid in the nth sampling, ci is the concentration of the receiving liquid in the ith sampling, and 2 is the sampling quantity; the test records are detailed in Table 3.

Test 7, sensory evaluation: 110 testers respectively use the masks of the examples 1-5, the comparative examples 1-5 and the comparative examples after cleaning the face every Monday, three, five and daily at night, and the effects of moisturizing, tendering, brightening, whitening and the like of the masks of the examples 1-5, the comparative examples 1-5 and the comparative examples are scored according to skin conditions and use feelings after using the masks for one month, and the effects are completely ineffective according to the lowest 0 score; the highest 5 points, i.e. the effect is prominent, scoring is performed from low to high, and the specific scoring conditions are shown in Table 4.

The specific test data for runs 1-7 are detailed in tables 2-4.

TABLE 2

According to the performance detection data of the examples 1-3 and the comparative example in the table 2, the mask liquid prepared by the application examples 1-3 has a strong inhibition effect on propionibacterium acnes, which is the main germ causing acne, and the mask liquid prepared by the application has a high inhibition rate on propionibacterium acnes, so that the mask liquid prepared by the application can effectively relieve pimple and acne problems; the facial mask liquid prepared in the embodiments 1-3 can obviously relieve the problem of facial skin redness after being used for two or three days, and has quick and efficient relieving and repairing effects; the prior commercial bio-peptide mask used in the comparative example has poor effect of relieving the problems of acne inflammation and facial redness. The facial mask liquid prepared in the embodiments 1-3 of the application is more excellent in the aspects of skin elasticity change and free radical removal compared with the facial mask liquid prepared in the comparative example, and the skin tendering and antioxidation effects of the facial mask liquid prepared in the application are further improved compared with the prior art.

Particularly, the skin moisture content of the mask liquid prepared in the embodiment 1-3 of the application is remarkably improved after 30min of application, and the skin moisture content can also be increased after 120min, but the phenomenon of skin moisture loss already occurs in 120min after the mask of the comparison example is used, which fully illustrates that the mask liquid prepared in the embodiment 1-3 of the application has longer lasting moisturizing and water-locking effects, can realize skin water circulation, and solves the problem of dry and rough skin.

In examples 4 and 5, the emollient and the skin conditioner defined in the present application were replaced with components capable of achieving similar functions, but compared with the components of the mask liquid in examples 1 to 3, which have already formed a matching system, the components after replacement cannot produce the excellent effects of the mask liquid in examples 1 to 3 on the skin in terms of efficacy, so that the connectivity and the matching between the substances in the mask liquid system of the present application are fully described, and the components cannot be replaced at will.

In comparative examples 1 to 5, the purslane extract, beta-glucan, nicotinamide, wheat bran extract and skin conditioning agent are replaced by equal amounts of water, and each performance of the mask liquid prepared in comparative examples 1 to 5 is reduced compared with that of the mask liquid prepared in examples 1 to 3, so that the effects of the mask liquid can be well reflected mainly by the synergistic interaction between the components, the activity of other components can be influenced by the lack of one component, the additional gain effect generated by the interaction of the components can be eliminated, the effects of the mask liquid are further influenced, and only by the combination and compounding of the components in the mask liquid, the effects of the components can be fully exerted, and the effects of the components are mutually optimal, so that the effects of the prepared mask liquid are outstanding.

TABLE 3 Table 3

According to the results of the experiment group and the comparison group in the table 3, comparison analysis can be carried out, after the experiment group and the comparison group respectively use the mask liquid prepared in the embodiment 3 of the application and the mask of the comparison example for face application, although the types of emulsion brands are different, the experiment group mouse skin using the mask liquid prepared in the embodiment 3 of the application shows better transdermal absorption effect on the emulsion, the absorption of the mouse skin of the comparison group on the emulsion in the same time is only half of the data of the experiment group, the mask liquid prepared in the application is fully reflected through the experiment, the mask liquid prepared in the application has better skin activating effect, after the mask prepared in the application is used for face application, the water emulsion is used for skin care, the absorption capacity of the skin on the water emulsion is greatly improved, the absorption capacity has better universality on skin care products of various brands, and the daily skin care maintenance of users is more facilitated, and the functionality and the practicability of the mask prepared in the application are also greatly improved.

TABLE 4 Table 4

Scoring of	Moisturizing agent	Skin care product	Lightening up	Whitening skin
					Example 1	4	3	3	3
Example 2	5	4	4	3
					Examples3	5	5	4	4
Example 4	4	4	3	3
					Example 5	4	4	3	3
Comparative example 1	4	3	3	2
					Comparative example 2	2	3	2	2
Comparative example 3	3	2	1	1
					Comparative example 4	2	3	2	2
Comparative example 5	3	2	2	2
					Comparative example	4	4	3	2

According to the scoring results of sensory evaluation of the examples, the comparative examples and the comparative examples in table 4, the mask liquid prepared in examples 1 to 3 of the present application has more efficacy than the prior art of the comparative examples, and the corresponding moisturizing, skin tendering, brightening and whitening performances also have good actual measurement promoting effects.

The foregoing are all preferred embodiments of the present application, and are not intended to limit the scope of the present application in any way, therefore: all equivalent changes in structure, shape and principle of this application should be covered in the protection scope of this application.

Claims

1. A biological peptide activation repairing mask liquid is characterized in that: comprises the following raw materials in percentage by mass:

and B phase: arginine 0.08-0.1%;

and C phase: 2.0 to 2.4 percent of antioxidant;

the balance of water;

wherein the hyaluronic acid comprises 4000-6000 daltons of small molecular hyaluronic acid and 100-130 ten thousand daltons of macromolecular hyaluronic acid, and the mass ratio of the small molecular hyaluronic acid to the macromolecular hyaluronic acid is 2:1;

the skin conditioner consists of 23-27wt% of hydrolyzed sweet tonsil protein, 2.3-2.7wt% of yeast, 0.08-0.12wt% of ethylhexyl glycerol and the balance of solvent;

the biological peptide composition comprises 0.011wt% of decapeptide-4, 0.011wt% of glycoprotein, 0.15-0.22wt% of disodium hydrogen phosphate, 0.8-1.2wt% of sodium chloride and the balance of water.

2. The biopeptide activation repair mask liquid according to claim 1, wherein: the emollient comprises rhodophyta extract, acrylic acid (ester) copolymer sodium and bis-PEG-15 methyl ether polydimethoxyalkane, wherein the mass ratio of the rhodophyta extract to the acrylic acid (ester) copolymer sodium to the bis-PEG-15 methyl ether polydimethoxyalkane is 1:4:10.

3. The biopeptide activation repair mask liquid according to claim 1, wherein: the preservative consists of 5-7wt% of octanoyl hydroxamic acid, 2-4wt% of ethylhexyl glycerol and the balance of propylene glycol.

4. The biopeptide activation repair mask liquid according to claim 1, wherein: the antioxidant comprises 1, 3-propanediol and p-hydroxyacetophenone, and the mass ratio of the 1, 3-propanediol to the p-hydroxyacetophenone is 10:1.

5. A method for preparing the biopeptides activating repair mask fluid according to any one of claims 1 to 4, which is characterized in that: the method comprises the following steps:

s1, mixing the phase A raw material and water, putting into an emulsifying pot, heating to 85 ℃, homogenizing, dispersing, uniformly stirring, and cooling to 50 ℃;

s2, independently heating the C-phase raw material to 60 ℃, dissolving and transparentizing, and adding the C-phase raw material into an emulsifying pot;