CN115363131A - Biological agent for silage total mixed ration fermentation and preparation method thereof - Google Patents

Biological agent for silage total mixed ration fermentation and preparation method thereof Download PDF

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CN115363131A
CN115363131A CN202211106707.1A CN202211106707A CN115363131A CN 115363131 A CN115363131 A CN 115363131A CN 202211106707 A CN202211106707 A CN 202211106707A CN 115363131 A CN115363131 A CN 115363131A
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silage
parts
fermentation
mixed ration
biological agent
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王诚
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Shandong Jianyuan Biological Technology Co ltd
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Shandong Jianyuan Biological Technology Co ltd
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Priority to ZA2023/05584A priority patent/ZA202305584B/en
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
    • A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
    • A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/113—Acidophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/41—Pediococcus
    • A23V2400/427—Pentosaceus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
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Abstract

The invention discloses a biological preparation for silage total mixed ration fermentation and a preparation method thereof. The biological agent remarkably reduces the pH value of the silage, improves the contents of organic acid and crude protein in the silage, and improves the mouthfeel of the silage total mixed ration through the synergistic effect of the cellulose decomposition microbial agent and the fermentation microbial agent.

Description

Biological agent for silage total mixed ration fermentation and preparation method thereof
Technical Field
The invention relates to the technical field of silage fermentation, in particular to a biological agent for silage total mixed ration fermentation and a preparation method thereof.
Background
The silage refers to greenish succulent feed which is preserved in a closed silage facility or through lactobacillus fermentation or by adopting chemical preparation or reducing water, and the silage is an effective method for preparing and storing the silage, root tubers and agricultural and sideline products. After the ensiling raw material is placed in a cellar, aerobic microorganisms and various enzymes attached to the raw material move by utilizing juice which is discharged by mechanical squeezing of the feed and is rich in nutrients such as carbohydrate and the like until oxygen in a container is exhausted and an anaerobic environment is formed for about 1-3 days, and then the breathing is stopped. Compared with other preparation methods, the ensiling can effectively preserve the contents of protein and vitamins, particularly carotene, in the feed; the feed is fermented, so that the flavor is fragrant, the feed is soft and juicy, and the palatability is good; the green feed which is redundant in summer and autumn can be stored for being used in winter and spring, and the balanced supply of nutrient substances is facilitated; the modulation method is simple and easy to master; is not limited by weather conditions; the taking is convenient and the taking can be carried out at any time; the storage space is smaller than that of hay, so that the storage field can be saved; the storage process is not influenced by wind, rain, sunshine and the like, and fire accidents such as spontaneous combustion and the like can not happen.
The ensiling additive can be mainly divided into two categories of biological additive and chemical additive. Lactic acid bacteria are the most commonly used biological additives, and can rapidly utilize fermentation substrates in the early stage of ensiling to generate lactic acid, reduce the pH value, inhibit the growth of harmful microorganisms and achieve the purpose of preserving feed. The commonly used chemical additives are mainly acids, such as formic acid, citric acid and the like, which are mainly used for rapidly reducing the pH value of the silage so as to achieve the aim of inhibiting fermentation.
At present, the silage production in China is still in the traditional stage, the utilization rate of raw materials is less than 10%, the secondary fermentation after silage is very serious, the silage utilization rate is low, and meanwhile, the technical problem of poor palatability exists, so that a biological agent for silage total mixed ration fermentation is urgently needed to solve the problems in the prior art.
Disclosure of Invention
Aiming at the prior art, the invention aims to provide a biological agent for silage total mixed ration fermentation and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a biological preparation for silage total mixed ration fermentation, which comprises the following raw materials in parts by weight: 12-20 parts of glucose, 10-16 parts of a fermentation microbial inoculum and 4-6 parts of a cellulose decomposition microbial inoculum.
Preferably, the fermentation inoculum comprises lactobacillus acidophilus, lactobacillus buchneri and pediococcus pentosaceus.
Further preferably, the ratio of the lactobacillus acidophilus to the lactobacillus buchneri to the pediococcus pentosaceus is (1.2-1.8): (1.0-2.0): (1.4-1.6).
More preferably, the total viable count of the fermentation inoculum is 3.6 multiplied by 10 5 – 5.4×10 5 cfu/g。
Preferably, the cellulose decomposition microbial inoculum is rumen albuginea.
More preferably, the number of viable bacteria of Ruminous albus is 1.2 × 10 5 – 1.8×10 5 cfu/g。
In a second aspect of the present invention, there is provided a method for preparing a biological agent for silage total mixed ration fermentation, comprising the steps of:
and uniformly mixing the glucose, the fermentation microbial inoculum and the cellulose decomposition microbial inoculum according to the weight part to obtain the biological preparation for the fermentation of the silage total mixed ration.
In a third aspect of the invention, there is provided the use of the biological agent in the preparation of a silage total mix ration.
Preferably, the silage raw materials are corn stalks and sweet potato vines.
The fourth aspect of the invention provides a preparation method of silage total mixed ration, which comprises the following steps:
(1) Collecting corn straws from a wax ripeness stage to a yellow ripeness stage and sweet potato vines 1-2 days before harvesting the sweet potatoes, removing rotten and dried stems and leaves in the corn straws, cutting the stems and leaves, and mixing the stems and leaves to obtain silage raw materials;
(2) Spreading out the silage raw materials and airing until the water content is 60-70%;
(3) Uniformly mixing the aired silage raw materials, the biological agent and the concentrated feed to obtain a mixture;
(4) And (3) paving a plastic film on the wall of the pit, stacking the mixture in the pit, compacting once every 50cm of the mixture is stacked, finally covering two layers of films on the top, compacting the splicing positions of different films by sand bags, and fermenting for 20-30 days to obtain the silage total mixed ration.
Preferably, in the step (3), the mass ratio of the silage raw materials, the concentrated feed and the biological agent is (8-10): (4-6): 1.
preferably, the concentrated feed in the step (3) is prepared from the following raw materials in parts by weight: 55-60 parts of corn, 7-9 parts of cottonseed meal, 4-6 parts of rapeseed meal, 8-10 parts of wheat bran, 10-16 parts of sodium chloride and 5 parts of premix.
Further preferably, the premix comprises the following components by taking the total weight of the total mixed ration as 1 kg: 10-20mg of copper, 100-200mg of manganese, 100-200mg of zinc, 0.4-0.6mg of cobalt, 0.4-0.8mg of selenium, 4-6mg of iodine, 16-18mg of calcium, 10-20 kiloIU of VA, 6-8 kiloIU of VD, and 40-80 kiloIU of VE.
Preferably, the compacted density in step (4) is from 500 to 580g/L.
Preferably, the fermentation temperature in step (4) is 22-26 ℃.
The invention has the beneficial effects that:
the biological agent for silage total mixed ration fermentation and the preparation method thereof provided by the invention are added in the process of mixing silage of corn straws and sweet potato vines, so that the generation of lactic acid in the silage process can be accelerated, the production of putrefying bacteria in the fermentation process can be inhibited, a good environment suitable for the growth of livestock is formed, the immune function of ruminants is improved, and the healthy growth of the ruminants is promoted; the silage prepared by the biological agent can avoid secondary fermentation; the biological preparation is added in the silage process, the pH value of the silage can be obviously reduced and the lactic acid content, butyric acid content and protein content of the silage are improved through the synergistic effect of the white rumen bacteria, the lactobacillus acidophilus, the lactobacillus buchneri and the pediococcus pentosaceus, and meanwhile, the glucose and the sodium chloride are added in the biological preparation, so that the balance of nutrition is ensured, the taste of the silage fully mixed ration is favorably improved, the feed intake of the ruminant is improved, the marketing time of the ruminant is shortened, and the practical value of the feed is increased.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
As described in the background art, the utilization rate of raw materials in the current production of silage is less than 10%, the secondary fermentation after silage is very serious, the silage utilization rate is low, and meanwhile, the ruminant feed intake is small due to poor palatability.
In order to make the technical solutions of the present application more clearly understood by those skilled in the art, the technical solutions of the present application will be described in detail below with reference to specific embodiments.
The test materials used in the examples of the present invention were all conventional in the art and commercially available.
The lactobacillus acidophilus (purchase source: china center for type culture Collection with the preservation number of CCTCC AB 2010208), lactobacillus buchneri (purchase source: china center for type culture Collection with the preservation number of CCTCC AB 2016284), pediococcus pentosaceus (purchase source: china center for type culture Collection with the preservation number of CCTCC CB 20081795) and ruminococcus albus (purchase source: aryobium sp, commodity product number of B89526) are included.
Example 1: preparation of biological agent for silage total mixed ration fermentation
The raw materials comprise: 12 parts of glucose, 10 parts of fermentation microbial inoculum and 4 parts of cellulose decomposition microbial inoculum.
The fermentation inoculum comprises lactobacillus acidophilus, lactobacillus buchneri and pediococcus pentosaceus, and the weight ratio of the fermentation inoculum is 1.2 5 cfu/g; the cellulose decomposition microbial inoculum is white rumen bacteria, and the viable count of the white rumen bacteria is 1.2 × 10 5 cfu/g。
And weighing and uniformly mixing the glucose, the fermentation microbial inoculum and the cellulose decomposition microbial inoculum according to the weight part to obtain the biological preparation for the silage total mixed ration fermentation.
Example 2: preparation of biological agent for silage total mixed ration fermentation
The raw materials comprise: 20 parts of glucose, 16 parts of fermentation microbial inoculum and 6 parts of cellulose decomposition microbial inoculum.
The fermentation inoculum comprises lactobacillus acidophilus, lactobacillus buchneri and pediococcus pentosaceus, and the weight ratio of the fermentation inoculum is 1.8 5 cfu/g; the cellulose decomposition microbial inoculum is white rumen bacteria, and the viable count of the white rumen bacteria is 1.8 × 10 5 cfu/g。
And weighing and uniformly mixing the glucose, the fermentation microbial inoculum and the cellulose decomposition microbial inoculum according to the weight part to obtain the biological preparation for the silage total mixed ration fermentation.
Example 3: preparation of biological agent for silage total mixed ration fermentation
The raw materials comprise: 16 parts of glucose, 13 parts of fermentation microbial inoculum and 5 parts of cellulose decomposition microbial inoculum.
The fermentation inoculum comprises lactobacillus acidophilus, lactobacillus buchneri and pediococcus pentosaceus, and the weight ratio of the fermentation inoculum is 1.5 5 cfu/g; the cellulose decomposition microbial inoculum is white rumen bacteria, and the viable count of the white rumen bacteria is 1.6 × 10 5 cfu/g。
And weighing and uniformly mixing the glucose, the fermentation microbial inoculum and the cellulose decomposition microbial inoculum according to the weight part to obtain the biological preparation for the silage total mixed ration fermentation.
Application example 1: preparation of silage total mixed ration
(1) Collecting corn straws from a wax ripeness stage to a yellow ripeness stage and sweet potato vines 1-2 days before harvesting the sweet potatoes, removing rotten and dried stems and leaves in the corn straws, cutting the stems and leaves, and mixing the stems and leaves to obtain silage raw materials;
(2) Spreading out the silage raw materials and airing until the water content is 60-70%;
(3) And (3) mixing the aired silage raw material, the biological agent prepared in the embodiment 3 and the concentrated feed according to a mass ratio of 9:5:1, uniformly mixing to obtain a mixture;
wherein the concentrated feed comprises 57 parts of corn, 8 parts of cottonseed meal, 5 parts of rapeseed meal, 9 parts of wheat bran, 14 parts of sodium chloride and 5 parts of premix;
the premix comprises the following components in percentage by total weight of 1 kg: 15mg of copper, 150mg of manganese, 150mg of zinc, 0.5mg of cobalt, 0.6mg of selenium, 5mg of iodine, 17mg of calcium, 15 thousand IU of VA, 7 thousand IU of VD, and 60 thousand IU of VE.
(4) Spreading a plastic film on the wall of the cellar, piling the mixture in the cellar once when the mixture is 50cm high, compacting once, finally covering two layers of films on the top, compacting the spliced parts of different films by sand bags, wherein the compaction density is 560g/L, and fermenting at 24 ℃ for 20 days to obtain the silage total mixed ration.
Comparative example 1:
the biological preparation comprises the following raw materials: 16 parts of glucose and 13 parts of fermentation inoculum.
The fermentation inoculum comprises lactobacillus acidophilus, lactobacillus buchneri and pediococcus pentosaceus, and the weight ratio of the fermentation inoculum is 1.5 5 cfu/g。
And weighing the glucose and the fermentation microbial inoculum according to the weight parts, and uniformly mixing to obtain the biological preparation for fermenting the silage total mixed ration.
(1) Collecting corn straws from a wax ripeness stage to a yellow ripeness stage and sweet potato vines 1-2 days before harvesting the sweet potatoes, removing rotten and dried stems and leaves in the corn straws, cutting the stems and leaves, and mixing the stems and leaves to obtain silage raw materials;
(2) Spreading out the silage raw materials and airing until the water content is 60-70%;
(3) And (3) mixing the aired silage raw material, the biological agent prepared in the embodiment 3 and the concentrated feed according to a mass ratio of 9:5:1, uniformly mixing to obtain a mixture;
wherein the concentrated feed comprises 57 parts of corn, 8 parts of cottonseed meal, 5 parts of rapeseed meal, 9 parts of wheat bran, 14 parts of sodium chloride and 5 parts of premix;
the premix comprises the following components by taking the total weight of the total daily mixed amount as 1 kg: 15mg of copper, 150mg of manganese, 150mg of zinc, 0.5mg of cobalt, 0.6mg of selenium, 5mg of iodine, 17mg of calcium, 15 thousand IU of VA, 7 thousand IU of VD, and 60 thousand IU of VE.
(4) Spreading a plastic film on the wall of the cellar, piling the mixture in the cellar once when the mixture is 50cm high, compacting once, finally covering two layers of films on the top, compacting the spliced parts of different films by sand bags, wherein the compaction density is 560g/L, and fermenting at 24 ℃ for 20 days to obtain the silage total mixed ration.
Comparative example 2:
the biological preparation comprises the following raw materials: 16 parts of glucose and 5 parts of cellulose decomposition microbial inoculum.
Wherein the cellulose decomposition microbial inoculum is Ruminous albus with viable count of 1.6 × 10 5 cfu/g。
And weighing the glucose, the sodium chloride and the cellulose decomposition microbial inoculum according to the weight parts, and uniformly mixing to obtain the biological preparation for the fermentation of the silage total mixed ration.
(1) Collecting corn stalks from a wax ripeness stage to a yellow ripeness stage and sweet potato vines 1-2 days before harvesting the sweet potatoes, removing rotten and dried stems and leaves in the corn stalks, cutting the stems and leaves, and mixing the stems and leaves to obtain a silage raw material;
(2) Spreading out the silage raw materials and airing until the water content is 60-70%;
(3) And (3) mixing the aired silage raw material, the biological agent prepared in the embodiment 3 and the concentrated feed according to a mass ratio of 9:5:1, uniformly mixing to obtain a mixture;
wherein the concentrated feed comprises 57 parts of corn, 8 parts of cottonseed meal, 5 parts of rapeseed meal, 9 parts of wheat bran, 14 parts of sodium chloride and 5 parts of premix;
the premix comprises the following components in percentage by total weight of 1 kg: 15mg of copper, 150mg of manganese, 150mg of zinc, 0.5mg of cobalt, 0.6mg of selenium, 5mg of iodine, 17mg of calcium, 15 thousand IU of VA, 7 thousand IU of VD, and 60 thousand IU of VE.
(4) Spreading a plastic film on the wall of the cellar, piling the mixture in the cellar once when the mixture is 50cm high, compacting once, finally covering two layers of films on the top, compacting the spliced parts of different films by sand bags, wherein the compaction density is 560g/L, and fermenting at 24 ℃ for 20 days to obtain the silage total mixed ration.
Comparative example 3:
the biological preparation comprises the following raw materials: 16 parts of glucose and 14 parts of sodium chloride.
And weighing glucose and sodium chloride according to the parts by weight, and uniformly mixing to obtain the biological preparation for fermenting the silage total mixed ration.
(1) Collecting corn straws from a wax ripeness stage to a yellow ripeness stage and sweet potato vines 1-2 days before harvesting the sweet potatoes, removing rotten and dried stems and leaves in the corn straws, cutting the stems and leaves, and mixing the stems and leaves to obtain silage raw materials;
(2) Spreading out the silage raw materials and airing until the water content is 60-70%;
(3) And (3) mixing the aired silage raw material, the biological agent prepared in the embodiment 3 and the concentrated feed according to a mass ratio of 9:5:1, uniformly mixing to obtain a mixture;
wherein the concentrated feed comprises 57 parts of corn, 8 parts of cottonseed meal, 5 parts of rapeseed meal, 9 parts of wheat bran, 14 parts of sodium chloride and 5 parts of premix;
the premix comprises the following components in percentage by total weight of 1 kg: 15mg of copper, 150mg of manganese, 150mg of zinc, 0.5mg of cobalt, 0.6mg of selenium, 5mg of iodine, 17mg of calcium, 15 thousand IU of VA, 7 thousand IU of VD, and 60 thousand IU of VE.
(4) Spreading a plastic film on the wall of the cellar, piling the mixture in the cellar once when the mixture is 50cm high, compacting once, finally covering two layers of films on the top, compacting the spliced parts of different films by sand bags, wherein the compaction density is 560g/L, and fermenting at 24 ℃ for 20 days to obtain the silage total mixed ration.
Test example 1
The silage total mixed ration obtained in application example 1 and comparative example 1-3 is taken respectively, the silage total mixed ration leachate is centrifuged, the organic acid content in the silage total mixed fermented ration is analyzed by using a high performance liquid chromatography analyzer model SHIMADZE-10A, and the crude protein content is measured by adopting a semi-micro Kjeldahl (kjeldahl) nitrogen determination method, wherein the silage total mixed ration is green after silage, has slight sour taste or fruit fragrance, is soft when being taken up and is not sticky, and is used as a silage standard, and the crude protein content is measured by adopting a semi-micro Kjeldahl (kjeldahl) nitrogen determination method, which is shown in Table 1.
TABLE 1 organic acid and crude protein content in silage total mixed fermentation ration for each group
Figure DEST_PATH_IMAGE001
As can be seen from table 1, the organic acid content and the crude protein content in the silage total mixed ration in application example 1 are much higher than those in comparative examples 1 to 3, and thus it can be seen that the biological agent of the present invention can significantly increase the organic acid content and the crude protein content in the silage by the synergistic effect of the fermentation inoculant and the cellulose inoculant.
Test example 2
Selecting 40 cows with the weight of about 200kg, dividing into 4 groups, feeding 10 cows in each group, respectively feeding silage total mixed fermentation ration prepared in application example 1 and silage total mixed fermentation ration prepared in comparative example 1-3, feeding for 30 days, weighing the initial weight and the milk yield in the morning of the first day of feeding, recording the average feed intake of each group of cows, and performing data comparison and analysis after 30 days, wherein the data comparison and analysis are shown in table 2.
TABLE 2 daily food intake and milk production by cows in each group
Figure DEST_PATH_IMAGE003
Test example 3
The silage total mixed ration of the same mass of application example 1 and comparative examples 1-3 was taken, and the time required for the first spoilage was recorded until the first spoilage occurred by opening the bag, as shown in table 3.
TABLE 3 time to first appearance of spoilage
Figure DEST_PATH_IMAGE005
As can be seen from tables 2 and 3, the daily average feed intake, daily gain, and daily average milk yield of each cow of the silage total mixed ration of application example 1 are significantly better than those of the silage total mixed ration of comparative examples 1 to 3, and the silage total mixed ration of application example 1 is easier to store and less prone to decay than those of the silage total mixed ration of comparative examples 1 to 3.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims (10)

1. A biological agent for silage total mixed ration fermentation is characterized by comprising the following raw materials in parts by weight:
12-20 parts of glucose, 10-16 parts of a fermentation microbial inoculum and 4-6 parts of a cellulose decomposition microbial inoculum;
the fermentation inoculum is lactobacillus acidophilus, lactobacillus buchneri and pediococcus pentosaceus;
the cellulose decomposition microbial inoculum is white rumen bacteria.
2. The biological agent according to claim 1, wherein the total viable count of said fermentation inoculum is 3.6 x 10 5 – 5.4×10 5 cfu/g, the viable count of the cellulose decomposition microbial inoculum is 1.2 multiplied by 10 5 – 1.8×10 5 cfu/g。
3. The biological agent according to claim 1, wherein the ratio of the lactobacillus acidophilus to the lactobacillus buchneri to the pediococcus pentosaceus is (1.2-1.8): (1.0-2.0): (1.4-1.6).
4. A method of preparing a biological agent as claimed in any one of claims 1 to 3, comprising the steps of:
and uniformly mixing the glucose, the fermentation microbial inoculum and the cellulose decomposition microbial inoculum according to the weight part to obtain the biological preparation.
5. Use of a biological agent according to any one of claims 1 to 3 in the preparation of a silage total mix ration.
6. A preparation method of silage total mixed ration is characterized by comprising the following steps:
(1) Collecting corn stalks and sweet potato vines, cutting and mixing to obtain silage raw materials;
(2) Airing the silage raw materials until the water content is 60-70%;
(3) Uniformly mixing the aired silage raw materials and concentrated feed with the biological agent of any one of claims 1-3 to obtain a mixture;
(4) And (4) piling the mixture in a cellar, compacting, and fermenting in a dark place to obtain the silage total mixed ration.
7. The method for preparing the silage total mixed ration according to claim 6, wherein the mass ratio of the silage raw material, the concentrated feed and the biological agent in the step (2) is (8-10): (4-6): 1.
8. the method for preparing the silage total mixed ration according to claim 6, wherein the concentrated feed comprises the following raw materials in parts by weight: 55-60 parts of corn, 7-9 parts of cottonseed meal, 4-6 parts of rapeseed meal, 8-10 parts of wheat bran, 10-16 parts of sodium chloride and 5 parts of premix.
9. The method for preparing the silage whole mixed ration according to claim 6, wherein the compacted density in the step (4) is 500 to 580g/L.
10. The method for preparing the silage whole mixed ration according to claim 6, wherein the fermentation temperature in the step (4) is 22-26 ℃, and the fermentation time is 20-30 days.
CN202211106707.1A 2022-09-13 2022-09-13 Biological agent for silage total mixed ration fermentation and preparation method thereof Pending CN115363131A (en)

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Publication number Priority date Publication date Assignee Title
WO2008073835A1 (en) * 2006-12-11 2008-06-19 Pioneer Hi-Bred International, Inc. Lactobacillus buchneri strain ln1284 and its use to improve aerobic stability of silage
CN112890019A (en) * 2021-02-04 2021-06-04 华中农业大学 Forage rape silage and preparation method thereof
CN113016945A (en) * 2021-04-19 2021-06-25 江苏赤山湖农业科技有限公司 Preparation method of silage total mixed fermentation ration

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008073835A1 (en) * 2006-12-11 2008-06-19 Pioneer Hi-Bred International, Inc. Lactobacillus buchneri strain ln1284 and its use to improve aerobic stability of silage
CN112890019A (en) * 2021-02-04 2021-06-04 华中农业大学 Forage rape silage and preparation method thereof
CN113016945A (en) * 2021-04-19 2021-06-25 江苏赤山湖农业科技有限公司 Preparation method of silage total mixed fermentation ration

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