CN115362778A

CN115362778A - Screening method and biological composition suitable for slope ecological improvement

Info

Publication number: CN115362778A
Application number: CN202211144716.XA
Authority: CN
Inventors: 陈德赟; 潘林涛; 吴建平; 石兴燕; 刘佳鑫
Original assignee: Yunnan University YNU
Current assignee: Yunnan University YNU
Priority date: 2022-09-20
Filing date: 2022-09-20
Publication date: 2022-11-22
Anticipated expiration: 2042-09-20
Also published as: CN115362778B

Abstract

The invention relates to a screening method and a biological combination suitable for slope ecological improvement, which comprises the following steps of 1) selecting a biological material A and preparing a biological material A suspension; step 2) selecting a biological material B and preparing a biological material B suspension; step 3), spraying suspension; step 4), shading; step 5), culturing; step 6), detecting the water holding capacity of the soil; step 7), detecting soil nutrient elements; step 8), detecting the biomass of the biological material A; step 9) analysis of variance. According to the method, the optimized biological combination is finally found through the screening method suitable for slope ecology improvement, and theoretical basis and technical guidance are provided for slope ecology improvement.

Description

Screening method and biological combination suitable for slope ecological improvement

Technical Field

The invention belongs to the technical field of environmental remediation and improvement, and particularly relates to a technical method for changing water holding capacity and nutrient elements of soil by colonizing a slope bare land with moss and microorganisms in a matching manner.

Background

The side slope generally refers to the general term of slope morphologies such as natural slopes, river and water bank slopes, tablelands, slide-flow-collapsing accumulation bodies, artificial slopes (formed by traffic roads, open-air mining, construction sites, foundation engineering and the like), and the like, and has the characteristics of large slope, difficulty in fixation of plants, soil impoverishment and low fertility, and the plants basically cannot grow and are easy to cause damages such as landslide and collapse. The side slope may cause various disasters such as weathering spalling, falling stones, mud running, falling blocks and falling stones, collapse, dumping, collapse, crumpling, slipping, slump, landslide, dislocation and the like. After the side slope is formed, soil on the side slope can be washed down when rainfall occurs, serious water and soil loss is caused, damage is caused to plants and the like at the toe of the slope, the slope is exposed and not attractive, dust is easy to raise, and effective ecological management is urgently needed.

At present, the road slope restoration has more herbaceous plants, and the ecological slope protection has relatively poor effect; the design and treatment measures of the side slope are single, and the side slope is greatly damaged by the natural environment; the slope vegetation recovery technology level is low, and the ecological recovery work is difficult. Side slope bare land is because the side slope soil horizon is infertile, and the side slope is big, can not keep soil water content, and this makes soil erosion and water loss serious, and ordinary plant is difficult the field planting, and perhaps even can the field planting, the side slope restoration of developing adopts the concrete more, the time of mode cost such as slabstone bank protection, and manpower and materials are also great, and the heat capacity is less, and not environmental protection, needs a fast green pioneer species to develop ecological improvement.

Disclosure of Invention

The invention aims to solve the defects of the problems and provides a method for improving the water holding capacity and the soil nutrition of slope soil.

The invention is realized by adopting the following technical scheme.

A screening method suitable for slope ecological improvement comprises the following steps:

step 1) selecting a biological material A and preparing a biological material A suspension;

step 2) selecting a biological material B and preparing a biological material B suspension;

step 3) spraying the suspension

Mixing the biological material A suspension and the biological material B suspension, and spraying the slope soil; setting comparison at the same time;

step 4) shading

Covering the soil surface with a shading net to avoid direct solar radiation until the biological material A grows, and removing the shading net;

step 5) cultivation

Placing in a greenhouse, and watering periodically;

step 6) soil water holding capacity detection

After one year of culture, making a small hole at the bottom of a PVC disc plastic box filled with soil, horizontally placing the small hole on a beaker, adding 200ml of tap water, standing until no water drops, and pouring the water in the beaker into a measuring cylinder for measurement; step 7) detection of soil nutrient elements

Detecting the difference of nutrient elements of the soil before and after culture, leaching the soil, and detecting total nitrogen, ammonium nitrogen, total phosphorus, quick-acting phosphorus and soluble organic carbon in the soil by using a ClevelChem 380 full-automatic intermittent chemical analyzer and a TOC (total organic carbon) instrument;

step 8) biological Material A Biomass detection

After culturing for one year, collecting, drying and weighing the biological material A in the PVC disc plastic box;

step 9) analysis of variance

The Excel 2019 is used for processing the early-stage experimental data, the R language is used for carrying out two-factor variance analysis, and the significance level of the data is set to be P <0.05.

When the biological material A is a biological species, when the biological material B is a biological species, and the biological material B is a microorganism; the test set-up was, based on the total suspension amount of 125 ml: ck: 125ml of agar suspension;

test 1: 25ml of biological material A suspension and 100ml of agar suspension;

test 2: 50ml of biological material A suspension and 75ml of agar suspension;

test 3: 100ml of biological material A suspension and 25ml of agar suspension;

test 4: 25ml of biological material A suspension, 25ml of biological material B suspension and 75ml of agar suspension;

test 5: 50ml of biological material A suspension, 25ml of biological material B suspension and 50ml of agar suspension;

test 6: 100ml of the suspension of the biological material A + 25ml of the suspension of the biological material B.

When the biological material A is two biological species, marked as A1 and A2, and when the biological material B is a biological species and the biological material B is a microorganism, the biological species is selected as a microorganism; the test set-up was, based on the total suspension amount of 125 ml: ck: 125ml of agar suspension;

test 1: 25ml of the biological material A1 suspension and 100ml of the agar suspension;

test 2: 50ml of biological material A1 suspension and 75ml of agar suspension;

test 3: 100ml of the biological material A1 suspension and 25ml of the agar suspension;

test 4: 25ml of biological material A2 suspension and 100ml of agar suspension;

test 5: 50ml of biological material A2 suspension and 75ml of agar suspension;

test 6: 100ml of the biological material A2 suspension and 25ml of the agar suspension;

test 7: 25ml of biological material A1 suspension, 25ml of biological material B suspension and 75ml of agar suspension;

test 8: 50ml of a biological material A1 suspension, 25ml of a biological material B suspension and 50ml of an agar suspension;

test 9: 100ml of biological material A1 suspension and 25ml of biological material B suspension;

test 10: 25ml of biological material A2 suspension, 25ml of biological material B suspension and 75ml of agar suspension;

test 11: 50ml of biological material A2 suspension, 25ml of biological material B suspension and 50ml of agar suspension;

test 12: 100ml of the suspension of the biological material A2 + 25ml of the suspension of the biological material B.

When the biological material A is two biological species, marked as A1 and A2, and when the biological material B is two biological species, marked as B1 and B2, and the biological material B is a microorganism; the test set-up was, based on the total suspension amount of 125 ml:

ck: 125ml of agar suspension;

test 2: 50ml of biological material A1 suspension and 75ml of agar suspension;

test 4: 25ml of biological material A2 suspension and 100ml of agar suspension;

test 5: 50ml of biological material A2 suspension and 75ml of agar suspension;

test 7: 25ml of biological material A1 suspension, 25ml of biological material B1 suspension and 75ml of agar suspension;

test 8: 50ml of a biological material A1 suspension, 25ml of a biological material B1 suspension and 50ml of an agar suspension;

test 9: 100ml of biological material A1 suspension and 25ml of biological material B1 suspension;

test 10: 25ml of biological material A2 suspension, 25ml of biological material B1 suspension and 75ml of agar suspension;

test 11: 50ml of a biological material A2 suspension, 25ml of a biological material B1 suspension and 50ml of an agar suspension;

test 12: 100ml of biological material A2 suspension and 25ml of biological material B1 suspension;

test 13: 25ml of biological material A1 suspension, 25ml of biological material B2 suspension and 75ml of agar suspension;

test 14: 50ml of biological material A1 suspension, 25ml of biological material B2 suspension and 50ml of agar suspension;

test 15: 100ml of the suspension of the biological material A1 + 25ml of the suspension of the biological material B2.

Test 16: 25ml of biological material A2 suspension, 25ml of biological material B2 suspension and 75ml of agar suspension;

test 17: 50ml of biological material A2 suspension, 25ml of biological material B2 suspension and 50ml of agar suspension;

test 18: 100ml of the suspension of the biological material A2 + 25ml of the suspension of the biological material B2.

A biological combination suitable for slope ecology improvement, the biological material A of the combination is Racomitrium anoplodonoides of sphagnum xanthum or Brachythelium thraustum of sphagnum crispum; the biological material B is Trichoderma viride or Bacillus subtilis.

According to the composition, the biological material A is a Brachyththecium thraustum of sphagnum fragranthum moss, and the biological material B is a composition of Bacillus subtilis.

The composition consists of 100mL of sphagnum crispum suspension and 25mL of bacillus subtilis suspension.

According to the invention, the biological material A is sphagnum lanomodonoides and the biological material B is a combination of Bacillus subtilis.

The composition consists of 100mL of sphagnum chrysosporium suspension and 25mL of bacillus subtilis suspension.

The invention has the beneficial effects that 1) the invention improves the ecological slope protection effect of the soil vegetation system, selects and configures plant species of biological slope protection engineering according to local conditions, reduces the cost and improves the environmental resilience.

2) The ecological restoration method for the slope can effectively improve the bare slope, accords with the development concept proposed by the government in recent years, can solve the problems of large-area bare slope left after artificial activities, water and soil loss caused by the bare slope, easy landslide and the like, and can beautify the environment.

3) The technical method can improve the water holding capacity and nutrient elements of the slope soil by adding the bryophyte and the microbial agent suspension, and can enable herbs, even shrubs and arbors to better colonize and grow to form succession.

4) The experimental result shows that the addition of the moss and the microbial inoculum suspension to the slope soil can obviously increase the contents of total nitrogen, ammonium nitrogen, total phosphorus and available phosphorus in the slope soil, and meanwhile, the addition of the microbial inoculum can effectively promote the colonization and growth of bryophytes, increase the biomass accumulation of the bryophytes and also increase the water holding capacity of the soil.

The invention is further explained below with reference to the drawings and the detailed description.

Drawings

FIG. 1 is a graph showing the effect of the blank control group (125mL agar suspension added, CK) of the present invention;

FIG. 2 is a graph showing the effect of adding 25ml of a sphagnum fragrans suspension (100mL of agar suspension added, BT-25) according to the invention;

FIG. 3 is a graph showing the effect of adding 50ml of a sphagnum fragrans suspension (75mL of agar suspension added, BT-50) according to the invention;

FIG. 4 is a graph showing the effect of adding 100ml of a sphagnum cristatum suspension (25mL of agar suspension, BT-100) according to the invention;

FIG. 5 is a graph showing the effect of adding 25mL of the Brachypodium crispum suspension and 25mL of Trichoderma viride (adding 75ml of agar suspension, BT + TV-25);

FIG. 6 is a graph showing the effect of adding 50mL of a Brachypodium crispum suspension and 25mL of a Trichoderma viride suspension (adding 50ml of an agar suspension, BT + TV-50);

FIG. 7 is a graph showing the effect of adding 100mL of a sphagnum cristatum suspension and 25mL of a trichoderma viride suspension (BT + TV-100) according to the present invention;

FIG. 8 is a graph showing the effect of adding 25mL of sphagnum fragrans suspension and 25mL of Bacillus subtilis suspension (75ml of agar suspension added, BT + BS-25);

FIG. 9 is a graph showing the effect of adding 50mL of the Brachytrium crispum suspension and 25mL of the Bacillus subtilis suspension (adding 50ml of agar suspension, BT + BS-50) according to the present invention;

FIG. 10 is a graph showing the effect of adding 100mL of a sphagnum fragrans suspension and 25mL of a bacillus subtilis suspension (BT + BS-100) according to the present invention;

FIG. 11 is a graph showing the effect of adding 25ml of Moss xanthum suspension (100mL of agar suspension added, RA-25) according to the present invention;

FIG. 12 is a graph showing the effect of adding 50ml of Moss xanthium suspension (75mL of agar suspension added, RA-50) according to the present invention;

FIG. 13 is a graph showing the effect of adding 100ml of Moss xanthium suspension (adding 25mL of agar suspension, RA-100) according to the present invention;

FIG. 14 is a graph showing the effect of the present invention when 25mL of sphagnum xanthum suspension and 25mL of Trichoderma viride suspension are added (addition of agar suspension 50ml, RA + TV-25);

FIG. 15 is a graph showing the effect of adding 50mL of sphagnum xanthum suspension and 25mL of Trichoderma viride suspension (adding 50ml of agar suspension, RA + TV-50) according to the present invention;

FIG. 16 is a graph showing the effect of adding 100mL of Moscomitrella xanthium suspension and 25mL of Trichoderma viride suspension (RA + TV-100) according to the present invention;

FIG. 17 is a graph showing the effect of the present invention when 25mL of Moss xanthium suspension and 25mL of Bacillus subtilis suspension (75ml of agar suspension, RA + BS-25) are added;

FIG. 18 is a graph showing the effect of adding 50mL of Moss xanthium suspension and 25mL of Bacillus subtilis suspension (adding agar suspension 50ml, RA + BS-50) according to the present invention;

FIG. 19 is a graph showing the effect of adding 100mL of Moscomitrella Moss suspension and 25mL of Bacillus subtilis suspension (RA + BS-100) according to the present invention.

Detailed Description

See fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7, fig. 8, fig. 9, fig. 10, fig. 11, fig. 12, fig. 13, fig. 14, fig. 15, fig. 16, fig. 17, fig. 18, fig. 19.

A method for improving the water holding capacity and the soil nutrition of slope soil comprises the following steps:

step 1) Moss selection and Moss suspension preparation

Selecting moss: selecting sphagnum xanthatum and sphagnum cristatum which are strong in environmental stress resistance (Racomitrium anomodonides) and sphagnum fragrans (Brachyththecium thrustum);

moss suspension preparation: respectively taking 110g of the two selected air-dried mosses, adding agar suspension (prepared by adding 1g of agar powder into 1L of water sterile water according to the proportion, boiling and cooling for later use) into a wall breaking machine, smashing and uniformly mixing, and respectively preparing 15L of each suspension of the sphagnum xanthatum and the sphagnum crispum;

step 2) microorganism selection and preparation of microbial inoculum suspension

And (3) selecting microorganisms: two microbial inoculums of Trichoderma viride (Trichoderma viride) and Bacillus subtilis (Bacillus subtilis) which can grow and propagate on barren and dry soil and are easy to obtain and low in price are selected;

preparation of a microbial inoculum suspension: adding 4g of each of the two selected microbial agents into suspension (prepared by adding 1g of agar powder into 1L of water sterile water according to a proportion and boiling, and cooling for later use) and uniformly mixing in a wall breaking machine to prepare 15L of each of trichoderma viride and bacillus subtilis suspension;

step 3) spraying the suspension

120g of slope soil is placed in a PVC disc plastic box with the height of 5cm and the diameter of 15cm, a moss suspension and a microbial inoculum suspension are mixed and are uniformly sprayed on the surface of the slope soil by a spray can, and the formula is as follows:

1. blank control group (agar suspension added 125mL, CK);

2. 25ml of a sphagnum cristatum suspension (100mL of agar suspension added, BT-25) was added;

3. add 50ml of the crinis carbonisatus suspension (75ml of agar suspension, bt-50);

4. 100ml of a brittle shoot moss suspension (25ml of agar suspension, BT-100) was added;

5. 25mL of the Brachymenium crispum suspension and 25mL of Trichoderma viride were added (75 mL of agar suspension added, BT + TV-25);

6. adding 50mL of the Brachymenium crispum suspension and 25mL of the trichoderma viride suspension (adding 50ml of agar suspension, BT + TV-50);

7. 100mL of a sphagnum cristatum suspension and 25mL of a trichoderma viride suspension (BT + TV-100) were added;

8. adding 25mL of sphagnum cristatum suspension and 25mL of bacillus subtilis suspension (adding 75ml of agar suspension, BT + BS-25);

9. adding 50mL of sphagnum cristatum suspension and 25mL of bacillus subtilis suspension (adding 50ml of agar suspension, BT + BS-50);

10. adding 100mL of the sphagnum fragilis suspension and 25mL of the bacillus subtilis suspension (BT + BS-100);

11. 25ml of Moss xanthium suspension (100mL of agar suspension added, RA-25) was added;

12. 50ml of the sphagnum gracile suspension (75ml of agar suspension added, RA-50);

13. 100ml of Moss xanthium suspension was added (25mL of agar suspension was added, RA-100);

14. adding 25mL of sphagnum xanthum suspension and 25mL of trichoderma viride suspension (addition of agar suspension 50ml, RA + TV-25);

15 add 50mL of the sphagnum xanthum suspension and 25mL of the trichoderma viride suspension (add agar suspension 50ml, RA + TV-50);

16. 100mL of the sphagnum xanthum suspension and 25mL of the trichoderma viride suspension (RA + TV-100) were added;

17. adding 25mL of sphagnum xanthum suspension and 25mL of bacillus subtilis suspension (addition of 75ml of agar suspension, RA + BS-25);

18. adding 50mL of Moss xanthium suspension and 25mL of Bacillus subtilis suspension (adding agar suspension 50ml, RA + BS-50);

19. 100mL of the sphagnum suspension and 25mL of the Bacillus subtilis suspension (RA + BS-100) were added.

125mL of suspension was added to each PVC disc plastic box, and 5 replicates were set up for each treatment.

Step 4) shading

Covering the soil surface with a shading net to avoid direct solar radiation until the moss grows, and removing the shading net.

Step 5) cultivation

Placing in a greenhouse, and watering regularly.

Step 6) soil water holding capacity detection

After one year of culture, a small hole is opened at the bottom of the PVC disc plastic box filled with soil, the PVC disc plastic box is horizontally placed on a beaker, 200ml of tap water is added, the PVC disc plastic box is kept still until no water drops, and the water in the beaker is poured into a measuring cylinder for measurement.

Step 7) detection of soil nutrient elements

Detecting the difference of nutrient elements of the soil before and after culture, leaching the soil, and detecting total nitrogen, ammonium nitrogen, total phosphorus, quick-acting phosphorus and soluble organic carbon of the soil by using a ClevelChem 380 full-automatic intermittent chemical analyzer and a TOC (total organic carbon) instrument.

Step 8) moss biomass detection

After one year of culture, moss in the PVC disc plastic box is collected, dried and weighed.

Step 9) analysis of variance

Early-stage experimental data are processed by using Excel 2019, two-factor variance analysis is performed by using R language, and the significance level of the data is set to be P <0.05.

The operation key points are as follows:

1. selecting bryophyte, adding agar suspension (prepared by adding 1g of agar powder into 1L of water sterile water according to a proportion, boiling, and cooling for later use), crushing and uniformly mixing in a wall breaking machine, and respectively preparing the bryophyte suspension;

2. selecting a microbial agent, adding agar suspension (prepared by adding 1g of agar powder into 1L of water sterile water according to a proportion, boiling, and cooling for later use), smashing and uniformly mixing in a wall breaking machine, and respectively preparing microbial agent suspension;

3. mixing the moss suspension and the microbial inoculum suspension, and uniformly spraying the moss suspension and the microbial inoculum suspension on the surface of the slope soil by using a spraying pot;

4. moss plants and microorganisms are selected to improve the water holding capacity and soil nutrient elements of the slope soil;

5. the water holding capacity of the slope soil and the improvement of soil nutrient elements are embodied by detecting the water holding capacity detection of the soil and the accumulation amount of soil nutrient elements and moss biomass.

Table 1 table of test results of the present invention

Water capacity of soil (%)

Total nitrogen (g/kg)

Ammonium nitrogen (g/kg)

Total phosphorus (g/kg)

Quick-acting phosphorus (g/kg)

Soluble organic carbon (g/kg)

Moss biomass (g)

CK

23.6±0.733

0.354±0.027

5.768±1.024

0.355±0.009

0.112±0.058

41.934±16.373

0.132±0.065

BT-25

72.8±0.270

1.019±0.059

10.201±1.751

0.319±0.003

0.162±0.023

40.725±14.927

0.764±0.561

BT-50

69.1±0.286

0.943±0.043

12.253±1.398

0.561±0.016

0.19±0.017

40.542±4.791

0.676±0.232

BT-100

65±0.381

0.929±0.109

12.677±0.984

0.666±0.013

0.17±0.023

25.447±3.742

0.992±0.504

BT+TV-25

68.8±0.502

0.88±0.030

10.997±1.201

0.653±0.006

0.13±0.02

32.911±7.531

1.148±0.5

BT+TV-50

61.1±0.820

0.619±0.051

13.223±2.251

0.738±0.023

0.109±0.003

38.407±15.431

2.19±0.403

BT+TV-100

61.1±0.378

0.896±0.039

12.552±2.699

0.705±0.011

0.212±0.025

35.246±11.038

1.58±0.425

BT+BS-25

78±0.430

0.451±0.051

9.364±0.969

0.75±0.026

0.186±0.004

36.473±4.515

2.492±1.215

BT+BS-50

67.1±0.512

0.376±0.063

10.165±3.662

0.763±0.034

0.143±0.012

30.999±3.822

3.492±1.386

BT+BS-100

75.8±0.594

0.396±0.051

12.565±1.055

0.591±0.013

0.169±0.013

27.308±5.063

3.854±1.947

RA-25

40.7±0.358

1.07±0.032

5.166±1.943

0.325±0.003

0.057±0.004

68.915±24.427

0.402±0.254

RA-50

48.9±0.589

1.06±0.096

9.504±4.192

0.518±0.019

0.136±0.021

23.264±14.807

0.206±0.125

RA-100

35.7±0.581

1.249±0.095

5.501±1.932

0.696±0.024

0.144±0.015

70.942±36.136

0.996±0.579

RA+TV-25

59±0.561

0.533±0.041

8.342±0.834

0.342±0.029

0.141±0.028

38.013±8.618

1±0.233

RA+TV-50

60.9±0.506

0.574±0.032

10.256±2.738

0.608±0.011

0.143±0.024

43.14±9.069

0.598±0.241

RA+TV-100

62±0.464

0.771±0.023

10.25±2.776

0.703±0.005

0.166±0.013

39.912±6.706

1.538±0.368

RA+BS-25

51.2±0.740

0.704±0.053

22.137±4.011

0.633±0.043

0.191±0.031

57.184±9.286

1.92±0.777

RA+BS-50

39.9±0.383

1.098±0.049

9.822±1.655

0.541±0.01

0.181±0.026

39.131±4.613

2.714±0.706

RA+BS-100

50±1.457

0.635±0.061

9.301±2.352

0.727±0.009

0.16±0.023

62.106±9.359

1.854±0.686

The concrete results are as follows:

the soil water-holding capacity can be obviously improved by adding the sphagnum fragrans and the bacillus subtilis, and the soil water-holding capacity is superior to that of sphagnum chrysosporium and trichoderma viride, and the sphagnum chrysosporium and the bacillus subtilis are matched.

The matching of the two mosses and the two microorganisms can improve the soil nutrient elements and increase the biomass of the mosses,

in conclusion, the sphagnum xanthatum suspension with high concentration is matched with bacillus subtilis (RA + BS-100) to achieve the best effect on the slope soil. The bacillus subtilis and the sphagnum xanthatum growing in a severe environment form biological crusts, so that the geochemical cycle of the soil is promoted, the nutrient elements of the soil are increased, and the growth of bryophytes is facilitated. The growth of the bryophyte promotes the accumulation of soil nutrient elements, forms virtuous cycle and further improves the slope soil.

Expanding:

1. the moss can be selected from other bryophytes with strong environmental stress resistance, the air-dried moss can also be selected from fresh bryophytes, and the concentration of the moss suspension can be selected from other concentrations;

2. the microbial agent can be selected from other better microbial agents, and the concentration of the microbial agent suspension can be selected from other concentrations;

3. other plastic containers can be selected for containing slope soil, and other volumes can be selected for adding the total suspension volume.

The foregoing is only a part of the specific embodiments of the present invention and specific details or common general knowledge in the schemes have not been described herein in more detail. It should be noted that the above-mentioned embodiments do not limit the present invention in any way, and all technical solutions obtained by means of equivalent substitution or equivalent transformation for those skilled in the art are within the protection scope of the present invention. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims

1. A screening method suitable for slope ecological improvement is characterized by comprising the following steps:

step 3) spraying the suspension

step 4) shading

step 5) cultivation

Placing in a greenhouse, and watering periodically;

step 6) soil water holding capacity detection

After one year of culture, making a small hole at the bottom of a PVC disc plastic box filled with soil, horizontally placing the small hole on a beaker, adding 200ml of tap water, standing until no water drops, and pouring the water in the beaker into a measuring cylinder for measurement;

step 7) detection of soil nutrient elements

step 8) biological Material A Biomass detection

step 9) analysis of variance

2. The screening method according to claim 1, wherein when the biological material a is a biological species, when the biological material B is a biological species, and the biological material B is a microorganism; the test set-up was, based on the total suspension amount of 125 ml:

ck: 125ml of agar suspension;

test 1: 25ml of biological material A suspension and 100ml of agar suspension;

test 2: 50ml of biological material A suspension and 75ml of agar suspension;

test 3: 100ml of biological material A suspension and 25ml of agar suspension;

test 6: 100ml of suspension of biological Material A + 25ml of suspension of biological Material B.

3. The screening method according to claim 1, wherein when the biological material a is two biological species, denoted as A1, A2, when the biological material B is one biological species, and the biological material B is a microorganism; the test set-up was, based on the total suspension amount of 125 ml:

ck: 125ml of agar suspension;

test 2: 50ml of biological material A1 suspension and 75ml of agar suspension;

test 4: 25ml of biological material A2 suspension and 100ml of agar suspension;

test 5: 50ml of biological material A2 suspension and 75ml of agar suspension;

test 8: 50ml of biological material A1 suspension, 25ml of biological material B suspension and 50ml of agar suspension;

4. The screening method according to claim 1, wherein when the biological material a is two biological species, denoted as A1, A2, and when the biological material B is two biological species, denoted as B1, B2, and the biological material B is a microorganism; the test set-up was, based on the total suspension of 125 ml:

ck: 125ml of agar suspension;

test 2: 50ml of biological material A1 suspension and 75ml of agar suspension;

test 4: 25ml of biological material A2 suspension and 100ml of agar suspension;

test 5: 50ml of biological material A2 suspension and 75ml of agar suspension;

test 8: 50ml of biological material A1 suspension, 25ml of biological material B1 suspension and 50ml of agar suspension;

test 11: 50ml of biological material A2 suspension, 25ml of biological material B1 suspension and 50ml of agar suspension;

test 12: 100ml of the suspension of the biological material A2 + 25ml of the suspension of the biological material B1.

test 15: 100ml of biological material A1 suspension and 25ml of biological material B2 suspension;

5. A biological combination suitable for slope ecology improvement, wherein the biological material a of the combination is sphagnum lecanitum anomodondoides or sphagnum crispatus brachythusticum; the biological material B is Trichoderma viride or Bacillus subtilis.

6. A biological combination suitable for slope ecology improvement according to claim 5, wherein the combination is that the biological material A is Brachyththecium brachyporum and the biological material B is a combination of Bacillus subtilis.

7. A biological combination suitable for slope ecology improvement according to claim 6, wherein the combination consists of 100mL of a Moscomitrella friable suspension and 25mL of a Bacillus subtilis suspension.

8. A biological combination suitable for slope ecology improvement according to claim 5, wherein the combination is the Moss sphaericus anomodonides, and the biological material B is a combination of Bacillus subtilis.

9. A biological combination suitable for slope ecology improvement according to claim 8, wherein the combination consists of 100mL of Moss sphaerica suspension and 25mL of Bacillus subtilis suspension.