CN115362778B - Screening method and biological combination suitable for slope ecological improvement - Google Patents
Screening method and biological combination suitable for slope ecological improvement Download PDFInfo
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Abstract
The invention relates to a screening method and a biological combination suitable for improving side slope ecology, which comprise the steps of 1) selecting biological materials A and preparing a biological material A suspension; step 2) selection of biological material B and preparation of biological material B suspension; step 3) spraying the suspension; step 4) shading; step 5) culturing; step 6) detecting the water holding capacity of the soil; step 7) detecting soil nutrient elements; step 8) biological material A biomass detection; step 9) analysis of variance. According to the invention, through the screening method suitable for slope ecological improvement, the optimal biological combination is found finally, and theoretical basis and technical guidance are provided for improving slope ecology.
Description
Technical Field
The invention belongs to the technical field of environmental remediation and improvement, and particularly relates to a technical method for changing water holding capacity and nutrient elements of soil by collocating moss and microorganisms to colonize a slope bare land.
Background
The side slope refers to the general name of slope forms such as natural slopes, river water bank slopes, tablelands, landslide fluid accumulation bodies, artificial side slopes (formed by traffic roads, open-pit mining, building sites, foundation engineering and the like), and the like. The side slope can cause various disasters such as weathering and peeling, flowing stone and mud, falling blocks and stones, collapsing, dumping, collapsing, crumbling, sliding and slumping, landslide, staggering and the like. After the slope is formed, soil on the slope is washed down during rainfall, serious water and soil loss is caused, plants on the slope feet and the like are damaged, the slope is exposed and is not attractive, dust is easily raised, and effective ecological management is urgently needed.
At present, the grass plants are most in the road slope restoration, and the ecological slope protection has relatively poor effect; the design and treatment measures of the side slope are single, and the side slope is greatly damaged by natural environment; the vegetation restoration technology level of the side slope is low, and the ecological restoration work is difficult. The bare land of the side slope is barren due to the soil layer of the side slope, the gradient of the side slope is large, the water content of the soil cannot be kept, the water and soil loss is serious, common plants are difficult to field plant, or the time spent in the manners of concrete, slurry rubble slope protection and the like is mainly used for restoring the side slope although the field planting can be carried out, the manpower and material resources are also large, the heat capacity is small, the environment is not protected, and a pioneer species which is fast and re-green is required to carry out ecological treatment.
Disclosure of Invention
The invention aims to solve the defects and provides a method for improving the water holding capacity and the soil nutrition of the slope soil.
The invention is realized by adopting the following technical scheme.
A screening method suitable for slope ecological improvement, said screening method comprising the steps of:
step 1) selection of biological material A and preparation of biological material A suspension;
step 2) selection of biological material B and preparation of biological material B suspension;
step 3) spraying the suspension
Mixing the biological material A suspension and the biological material B suspension, and then spraying the side slope soil; setting a comparison at the same time;
step 4) shading
Covering the soil surface with a shading net to avoid direct sunlight until biological material A grows, and removing the shading net;
step 5) cultivation
Placing in a greenhouse, and watering regularly;
step 6) soil moisture content detection
After one year of culture, the bottom of a plastic box with a soil PVC disc is perforated, the plastic box is horizontally placed on a beaker, 200ml of tap water is added for standing until the plastic box is free from dripping, and water in the beaker is poured into a measuring cylinder for measurement;
step 7) soil nutrient element detection
Detecting the difference of nutrient elements of the soil before and after culture, leaching the soil, and respectively detecting total nitrogen, ammonium nitrogen, total phosphorus, quick-acting phosphorus and soluble organic carbon of the soil by a ClevelChem 380 full-automatic intermittent chemical analyzer and a TOC (total organic carbon) instrument;
step 8) biological Material A biomass detection
After one year of culture, the biological material A in the PVC disc plastic box is collected, dried and weighed;
step 9) analysis of variance
Processing early experimental data by Excel 2019, performing two-factor analysis of variance by using R language, and setting the data significance level as followsP<0.05。
In the invention, when the biological material A is a biological species, when the biological material B is a biological species and the biological material B is a microorganism; the test was set to 125ml total suspension:
ck: 125ml of agar suspension;
test 1: 25ml of biological material A suspension plus 100ml of agar suspension;
test 2: 50ml of biological material A suspension plus 75ml of agar suspension;
test 3: 100ml of biological material A suspension and 25ml of agar suspension;
test 4: 25ml of biological material A suspension, 25ml of biological material B suspension and 75ml of agar suspension;
test 5: 50ml of biological material A suspension, 25ml of biological material B suspension and 50ml of agar suspension;
test 6: 100ml of biological material A suspension and 25ml of biological material B suspension.
In the invention, when the biological material A is two biological species, which are marked as A1 and A2, and when the biological material B is one biological species, the biological material B is a microorganism; the test was set to 125ml total suspension:
ck: 125ml of agar suspension;
test 1: 25ml of biological material A1 suspension+100 ml of agar suspension;
test 2: 50ml of biological material A1 suspension plus 75ml of agar suspension;
test 3: 100ml of biological material A1 suspension plus 25ml of agar suspension;
test 4: 25ml of biological material A2 suspension+100 ml of agar suspension;
test 5: 50ml of biological material A2 suspension plus 75ml of agar suspension;
test 6: 100ml of biological material A2 suspension plus 25ml of agar suspension;
test 7: 25ml of biological material A1 suspension, 25ml of biological material B suspension and 75ml of agar suspension;
test 8: 50ml of biological material A1 suspension, 25ml of biological material B suspension and 50ml of agar suspension;
test 9: 100ml of biological material A1 suspension and 25ml of biological material B suspension;
test 10: 25ml of biological material A2 suspension, 25ml of biological material B suspension and 75ml of agar suspension;
test 11: 50ml of biological material A2 suspension, 25ml of biological material B suspension and 50ml of agar suspension;
test 12: 100ml of biological material A2 suspension + 25ml of biological material B suspension.
In the invention, when the biological material A is two biological species and is marked as A1 and A2, when the biological material B is two biological species and is marked as B1 and B2, and the biological material B is microorganism; the test was set to 125ml total suspension:
ck: 125ml of agar suspension;
test 1: 25ml of biological material A1 suspension+100 ml of agar suspension;
test 2: 50ml of biological material A1 suspension plus 75ml of agar suspension;
test 3: 100ml of biological material A1 suspension plus 25ml of agar suspension;
test 4: 25ml of biological material A2 suspension+100 ml of agar suspension;
test 5: 50ml of biological material A2 suspension plus 75ml of agar suspension;
test 6: 100ml of biological material A2 suspension plus 25ml of agar suspension;
test 7: 25ml of biological material A1 suspension, 25ml of biological material B1 suspension and 75ml of agar suspension;
test 8: 50ml of biological material A1 suspension, 25ml of biological material B1 suspension and 50ml of agar suspension;
test 9: 100ml of biological material A1 suspension and 25ml of biological material B1 suspension;
test 10: 25ml of biological material A2 suspension, 25ml of biological material B1 suspension and 75ml of agar suspension;
test 11: 50ml of biological material A2 suspension, 25ml of biological material B1 suspension and 50ml of agar suspension;
test 12: 100ml of biological material A2 suspension and 25ml of biological material B1 suspension;
test 13: 25ml of biological material A1 suspension, 25ml of biological material B2 suspension and 75ml of agar suspension;
test 14: 50ml of biological material A1 suspension, 25ml of biological material B2 suspension and 50ml of agar suspension;
test 15: 100ml of biological material A1 suspension and 25ml of biological material B2 suspension.
Test 16: 25ml of biological material A2 suspension, 25ml of biological material B2 suspension and 75ml of agar suspension;
test 17: 50ml of biological material A2 suspension, 25ml of biological material B2 suspension and 50ml of agar suspension;
test 18: 100ml of biological material A2 suspension + 25ml of biological material B2 suspension.
The invention relates to a biological combination suitable for improving side slope ecology, wherein the biological material A is moss of yellow sand mossRacomitrium anomodontoidesOr bryophyte with crisp branchesBrachythecium thraustumThe method comprises the steps of carrying out a first treatment on the surface of the The biological material B is Trichoderma virideTrichoderma virideOr bacillus subtilisBacillus subtilis。
The biological material A of the invention is moss of Physcomitrella fragransBrachythecium thraustumThe biological material B is bacillus subtilisBacillus subtilisIs a combination of (a) and (b).
The combination of the invention consisted of 100 mL. Fragrans moss suspension and 25mL of bacillus subtilis suspension.
The biological material A of the invention is moss of yellow sand mossRacomitrium anomodontoidesThe biological material B is bacillus subtilisBacillus subtilisIs a combination of (a) and (b).
The combination of the invention consisted of 100mL moss yellow moss suspension and 25mL bacillus subtilis suspension.
The invention has the beneficial effects that 1) the ecological slope protection effect of the soil vegetation system is improved, the plant types of the biological slope protection engineering are selected and configured according to local conditions, the cost is reduced, and the environmental resilience is improved.
2) The ecological restoration method can effectively improve the exposed slope, can solve the problems of exposed large-area slope and soil erosion and easy landslide caused by artificial activities, and can beautify the environment.
3) The technical method can improve the water holding capacity and nutrient elements of the soil of the side slope by adding the bryophyte and microbial agent suspension, and can cause herbs, even shrubs and arbors to better colonize and grow to form succession.
4) Experimental results show that the addition of the moss and microbial inoculum suspension to the side slope soil can obviously increase the contents of total nitrogen, ammonium nitrogen, total phosphorus and quick-acting phosphorus in the side slope soil, and meanwhile, the microbial inoculum can effectively promote the colonization and growth of moss plants, increase the biomass accumulation of the moss plants and increase the water holding capacity of the soil.
The invention is further explained below with reference to the drawings and the detailed description.
Drawings
FIG. 1 is a graph showing the effect of a blank control group (125 mL of an agar suspension added) according to the present invention;
FIG. 2 is a graph showing the effect of adding 25ml of the Physcomitrella patens suspension (100 mL of agar suspension added, BT-25);
FIG. 3 is a graph showing the effect of adding 50ml of the Physcomitrella patens suspension (75 mL of agar suspension added, BT-50) according to the present invention;
FIG. 4 is a graph showing the effect of adding 100ml of the Physcomitrella patens suspension (adding 25mL of agar suspension, BT-100) according to the present invention;
FIG. 5 is a graph showing the effect of adding 25mL of the Physcomitrella patens suspension and 25mL of Trichoderma viride (75 mL of agar suspension, BT+TV-25);
FIG. 6 is a graph showing the effect of adding 50mL of a Physcomitrella patens suspension and 25mL of a Trichoderma viride suspension (50 mL of an agar suspension, BT+TV-50);
FIG. 7 is a graph showing the effect of adding 100 mL. Fragrans suspension and 25mL Trichoderma viride suspension (BT+TV-100) in accordance with the present invention;
FIG. 8 is a graph showing the effect of adding 25mL. Fragrans suspension and 25mL of Bacillus subtilis suspension (adding 75mL of agar suspension, BT+BS-25) according to the present invention;
FIG. 9 is a graph showing the effect of adding 50 mL. Fragrans suspension and 25mL of Bacillus subtilis suspension (50 mL of agar suspension, BT+BS-50);
FIG. 10 is a graph showing the effect of adding 100 mL. Fragrans suspension and 25mL of Bacillus subtilis suspension (BT+BS-100) according to the present invention;
FIG. 11 is a graph showing the effect of adding 25ml yellow sand moss suspension (100 mL of agar suspension added, RA-25) according to the present invention;
FIG. 12 is a graph showing the effect of adding 50ml yellow sand moss suspension (75 mL of agar suspension added, RA-50) according to the present invention;
FIG. 13 is a graph showing the effect of adding 100ml yellow sand moss suspension (25 mL of agar suspension added, RA-100) according to the present invention;
FIG. 14 is a graph showing the effect of adding 25mL yellow moss suspension and 25mL Trichoderma viride suspension (50 mL of agar suspension, RA+TV-25) in accordance with the present invention;
FIG. 15 is a graph showing the effect of adding 50mL yellow moss suspension and 25mL Trichoderma viride suspension (50 mL of agar suspension, RA+TV-50) in accordance with the present invention;
FIG. 16 is a graph showing the effect of adding 100mL yellow moss suspension and 25mL Trichoderma viride suspension (RA+TV-100) in accordance with the present invention;
FIG. 17 is a graph showing the effect of adding 25mL yellow sand moss suspension and 25mL of Bacillus subtilis suspension (adding 75mL of agar suspension, RA+BS-25) according to the present invention;
FIG. 18 is a graph showing the effect of adding 50mL yellow sand moss suspension and 25mL of Bacillus subtilis suspension (50 mL of agar suspension, RA+BS-50);
FIG. 19 is a graph showing the effect of adding 100mL yellow sand moss suspension and 25mL of Bacillus subtilis suspension (RA+BS-100) according to the present invention.
Detailed Description
See fig. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19.
A method for improving soil water holding capacity and soil nutrition of a side slope comprises the following steps:
step 1) moss selection and moss suspension preparation
Moss selection: selecting yellow sand moss with stronger environmental stress resistanceRacomitrium anomodontoides) Radix seu cortex Dictamni RadicisBrachythecium thraustum）；
Preparing moss suspension: adding 110g of the selected two air-dried moss into an agar suspension (prepared by boiling 1L of water sterile water and 1g of agar powder according to a proportion, and cooling for standby), crushing and uniformly mixing in a wall breaking machine to prepare 15L of yellow moss and yellow moss brittle branch suspension respectively;
step 2) microorganism selection and preparation of microbial agent suspension
Microorganism selection: selecting green trichoderma which can grow and reproduce on barren and dry soil, is easy to obtain and has low priceTrichoderma viride) And bacillus subtilis @Bacillus subtilis) Two bacterial agents;
preparation of a microbial inoculum suspension: adding 4g of each of the two selected microbial agents into suspension (prepared by boiling 1L of water sterile water and 1g of agar powder according to a proportion, and cooling for standby), and uniformly mixing in a wall breaking machine to prepare 15L of each of trichoderma viride and bacillus subtilis suspension;
step 3) spraying the suspension
The method comprises the steps of placing 120g of side slope soil in a PVC disc plastic box with the height of 5cm and the diameter of 15 and cm, mixing moss suspension and microbial inoculum suspension, uniformly spraying the moss suspension and microbial inoculum suspension on the surface of the side slope soil by using a watering can, and matching as follows:
1. blank (125 mL of agar suspension added, CK);
2. add 25ml Physcomitrella patens suspension (adding agar suspension 100mL, BT-25);
3. 50ml of a Physcomitrella patens suspension (75 mL of agar suspension, BT-50) was added;
4. 100ml of a Physcomitrella patens suspension (25 mL of agar suspension, BT-100) was added;
5. 25mL of a Physcomitrella crispa suspension and 25mL of Trichoderma viride (75 mL of an agar suspension, BT+TV-25) were added;
6. 50mL of a Physcomitrella patens suspension and 25mL of a Trichoderma viride suspension (50 mL of agar suspension, BT+TV-50) were added;
7. 100mL of a moss crispa suspension and 25mL of a trichoderma viride suspension (bt+tv-100) were added;
8. 25mL of a Physcomitrella patens suspension and 25mL of a Bacillus subtilis suspension (75 mL of an agar suspension, BT+BS-25) were added;
9. 50mL of a Physcomitrella patens suspension and 25mL of a Bacillus subtilis suspension (50 mL of an agar suspension, BT+BS-50) were added;
10. 100mL of a Physcomitrella patens suspension and 25mL of a Bacillus subtilis suspension (BT+BS-100) were added;
11. add 25ml yellow sand moss suspension (add agar suspension 100mL, RA-25);
12. 50ml yellow sphagnum suspension (75 mL of agar suspension, RA-50) was added;
13. add 100ml yellow sphagnum suspension (25 mL of agar suspension, RA-100);
14. 25mL yellow moss suspension and 25mL Trichoderma viride suspension (50 mL of agar suspension, RA+TV-25) were added;
15 adding 50mL yellow moss suspension and 25mL trichoderma viride suspension (50 mL agar suspension, ra+tv-50);
16. 100mL yellow sand moss suspension and 25mL of Trichoderma viride suspension (RA+TV-100) were added;
17. 25mL yellow moss suspension and 25mL of Bacillus subtilis suspension (75 mL of agar suspension, RA+BS-25) were added;
18. 50mL yellow moss suspension and 25mL of Bacillus subtilis suspension (50 mL of agar suspension, RA+BS-50) were added;
19. 100mL yellow sand moss suspension and 25mL of Bacillus subtilis suspension (RA+BS-100) were added.
125mL suspensions were added to each PVC disc plastic box and 5 replicates were set for each treatment.
Step 4) shading
Covering the soil surface with a shading net to avoid direct sunlight until moss grows, and removing the shading net.
Step 5) cultivation
Placing in a greenhouse, and watering regularly.
Step 6) soil moisture content detection
After one year of incubation, the bottom of the plastic box with the soil PVC disk was perforated, placed horizontally on a beaker, and 200ml of tap water was added to stand until it no longer dropped, and the water in the beaker was poured into a measuring cylinder for measurement.
Step 7) soil nutrient element detection
Detecting the difference of nutrient elements of the soil before and after culture, leaching the soil, and respectively detecting the total nitrogen, ammonium nitrogen, total phosphorus, quick-acting phosphorus and soluble organic carbon of the soil by a ClevelChem 380 full-automatic intermittent chemical analyzer and a TOC instrument.
Step 8) moss biomass detection
After one year of culture, moss in the PVC disc plastic box is collected, dried and weighed.
Step 9) analysis of variance
By Excel 2019 processes the early experimental data, performs two-factor analysis of variance by using R language, and sets the data significance level as followsP<0.05。
The operation key points are as follows:
1. adding moss plants into agar suspension (prepared by boiling 1L of water and 1g of agar powder in proportion, and cooling for later use), crushing and mixing in a wall breaking machine, and respectively preparing moss suspension;
2. selecting microbial agent, adding agar suspension (prepared by boiling 1L of water sterile water and 1g of agar powder in proportion, cooling for later use), breaking in a wall breaking machine, and mixing to obtain microbial agent suspension;
3. mixing the moss suspension and the microbial inoculum suspension, and uniformly spraying the mixture on the surface of slope soil by using a watering can;
4. the bryophyte and microorganism are selected to improve the soil water holding capacity and soil nutrient elements of the side slope;
5. the improvement of the soil water holding capacity and the soil nutrient elements of the side slope is embodied by detecting the soil water holding capacity detection and the accumulation of the soil nutrient elements and the moss biomass.
Table 1 table of test results according to the embodiment of the present invention
Specific results:
the addition of the physcomitrella patens and the bacillus subtilis can obviously increase the water holding capacity of soil, and is superior to the collocation of the physcomitrella patens and the trichoderma viride, and the physcomitrella patens and the bacillus subtilis.
The collocation of two mosses and two microorganisms can improve the nutrient elements of soil and increase the biomass of mosses,
in combination, the high concentration yellow sand moss suspension and the bacillus subtilis are matched (RA+BS-100) to have the best effect on the side slope soil. Mainly because bacillus subtilis and yellow sand moss growing in severe environment form biological crust, the geochemical circulation of soil is promoted, the nutrient elements of the soil are increased, and the growth of moss plants is facilitated. The growth of moss plant promotes the accumulation of soil nutrient elements to form virtuous circle, thereby improving the side slope soil.
Expansion:
1. the moss of the invention can be selected from other moss plants with stronger environmental stress resistance, fresh moss plants can be selected from air-dried moss, and the concentration of the moss suspension can be selected from other concentrations;
2. the microbial agent can be selected from other better microbial agents, and the concentration of the microbial agent suspension can be selected from other concentrations;
3. other plastic containers may be selected for Cheng Fangbian slope soil and other volumes may be selected for addition to the total suspension volume.
What has been described above is only a partial embodiment of the invention, and the details or common sense of the knowledge in the scheme are not described here too much. It should be noted that the above embodiments do not limit the present invention in any way, and it is within the scope of the present invention for those skilled in the art to obtain the technical solution by equivalent substitution or equivalent transformation. The protection scope of the present application shall be subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (9)
1. The screening method suitable for improving the ecology of the side slope is characterized by comprising the following steps of:
step 1) selection of biological material A and preparation of biological material A suspension;
step 2) selection of biological material B and preparation of biological material B suspension;
step 3) spraying the suspension
Mixing the biological material A suspension and the biological material B suspension, and then spraying the side slope soil; setting a comparison at the same time;
step 4) shading
Covering the soil surface with a shading net to avoid direct sunlight until biological material A grows, and removing the shading net;
step 5) cultivation
Placing in a greenhouse, and watering regularly;
step 6) soil moisture content detection
After one year of culture, the bottom of a plastic box with a soil PVC disc is perforated, the plastic box is horizontally placed on a beaker, 200ml of tap water is added for standing until the plastic box is free from dripping, and water in the beaker is poured into a measuring cylinder for measurement;
step 7) soil nutrient element detection
Detecting the difference of nutrient elements of the soil before and after culture, leaching the soil, and respectively detecting total nitrogen, ammonium nitrogen, total phosphorus, quick-acting phosphorus and soluble organic carbon of the soil by a ClevelChem 380 full-automatic intermittent chemical analyzer and a TOC (total organic carbon) instrument;
step 8) biological Material A biomass detection
After one year of culture, the biological material A in the PVC disc plastic box is collected, dried and weighed;
step 9) analysis of variance
Processing early experimental data by Excel 2019, performing two-factor analysis of variance by using R language, and setting the data significance level as followsP <0.05。
2. The screening method of claim 1, wherein when biological material a is a biological species, when biological material B is a biological species, and biological material B is a microorganism; the test was set to 125ml total suspension:
ck: 125ml of agar suspension;
test 1: 25ml of biological material A suspension plus 100ml of agar suspension;
test 2: 50ml of biological material A suspension plus 75ml of agar suspension;
test 3: 100ml of biological material A suspension and 25ml of agar suspension;
test 4: 25ml of biological material A suspension, 25ml of biological material B suspension and 75ml of agar suspension;
test 5: 50ml of biological material A suspension, 25ml of biological material B suspension and 50ml of agar suspension;
test 6: 100ml of biological material A suspension and 25ml of biological material B suspension.
3. The screening method according to claim 1, wherein when biological material a is two biological species, denoted as A1, A2, when biological material B is one biological species, and biological material B is a microorganism; the test was set to 125ml total suspension:
ck: 125ml of agar suspension;
test 1: 25ml of biological material A1 suspension+100 ml of agar suspension;
test 2: 50ml of biological material A1 suspension plus 75ml of agar suspension;
test 3: 100ml of biological material A1 suspension plus 25ml of agar suspension;
test 4: 25ml of biological material A2 suspension+100 ml of agar suspension;
test 5: 50ml of biological material A2 suspension plus 75ml of agar suspension;
test 6: 100ml of biological material A2 suspension plus 25ml of agar suspension;
test 7: 25ml of biological material A1 suspension, 25ml of biological material B suspension and 75ml of agar suspension;
test 8: 50ml of biological material A1 suspension, 25ml of biological material B suspension and 50ml of agar suspension;
test 9: 100ml of biological material A1 suspension and 25ml of biological material B suspension;
test 10: 25ml of biological material A2 suspension, 25ml of biological material B suspension and 75ml of agar suspension;
test 11: 50ml of biological material A2 suspension, 25ml of biological material B suspension and 50ml of agar suspension;
test 12: 100ml of biological material A2 suspension + 25ml of biological material B suspension.
4. The screening method according to claim 1, wherein when biological material a is two biological species, designated A1, A2, when biological material B is two biological species, designated B1, B2, and biological material B is a microorganism; the test was set to 125ml total suspension:
ck: 125ml of agar suspension;
test 1: 25ml of biological material A1 suspension+100 ml of agar suspension;
test 2: 50ml of biological material A1 suspension plus 75ml of agar suspension;
test 3: 100ml of biological material A1 suspension plus 25ml of agar suspension;
test 4: 25ml of biological material A2 suspension+100 ml of agar suspension;
test 5: 50ml of biological material A2 suspension plus 75ml of agar suspension;
test 6: 100ml of biological material A2 suspension plus 25ml of agar suspension;
test 7: 25ml of biological material A1 suspension, 25ml of biological material B1 suspension and 75ml of agar suspension;
test 8: 50ml of biological material A1 suspension, 25ml of biological material B1 suspension and 50ml of agar suspension;
test 9: 100ml of biological material A1 suspension and 25ml of biological material B1 suspension;
test 10: 25ml of biological material A2 suspension, 25ml of biological material B1 suspension and 75ml of agar suspension;
test 11: 50ml of biological material A2 suspension, 25ml of biological material B1 suspension and 50ml of agar suspension;
test 12: 100ml of biological material A2 suspension and 25ml of biological material B1 suspension;
test 13: 25ml of biological material A1 suspension, 25ml of biological material B2 suspension and 75ml of agar suspension;
test 14: 50ml of biological material A1 suspension, 25ml of biological material B2 suspension and 50ml of agar suspension;
test 15: 100ml of biological material A1 suspension and 25ml of biological material B2 suspension;
test 16: 25ml of biological material A2 suspension, 25ml of biological material B2 suspension and 75ml of agar suspension;
test 17: 50ml of biological material A2 suspension, 25ml of biological material B2 suspension and 50ml of agar suspension;
test 18: 100ml of biological material A2 suspension + 25ml of biological material B2 suspension.
5. A biological composition for slope ecology improvement selected by the screening method of claim 1, wherein the biological material a in the biological composition is bryophyteRacomitrium anomodontoidesOr bryophyte with crisp branchesBrachythecium thraustumThe method comprises the steps of carrying out a first treatment on the surface of the The biological material B in the biological combination is Trichoderma virideTrichoderma virideOr bacillus subtilisBacillus subtilis。
6. The biological combination for slope ecology improvement of claim 5 wherein the biological material a in the biological combination is bryophyteBrachythecium thraustumThe biological material B in the biological combination is bacillus subtilisBacillus subtilisIs a combination of (a) and (b).
7. The biological combination according to claim 6, characterized in that it consists of 100mL moss crispatus suspension and 25mL bacillus subtilis suspension.
8. The biological combination for slope ecology improvement of claim 5 wherein biological material a in the biological combination is moss yellow sandRacomitrium anomodontoidesThe biological material B in the biological combination is bacillus subtilisBacillus subtilisIs a combination of (a) and (b).
9. The biological combination for slope ecology improvement of claim 8 consisting of 100mL yellow moss suspension and 25mL bacillus subtilis suspension.
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