CN115316347A - Spodoptera frugiperda control method - Google Patents
Spodoptera frugiperda control method Download PDFInfo
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- CN115316347A CN115316347A CN202211014475.7A CN202211014475A CN115316347A CN 115316347 A CN115316347 A CN 115316347A CN 202211014475 A CN202211014475 A CN 202211014475A CN 115316347 A CN115316347 A CN 115316347A
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- larvae
- spodoptera frugiperda
- pyemotes
- zhonghuajia
- culture dish
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- 241000256251 Spodoptera frugiperda Species 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 17
- 241000238706 Pyemotes Species 0.000 claims abstract description 60
- 235000013601 eggs Nutrition 0.000 claims abstract description 24
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 13
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 13
- 235000012015 potatoes Nutrition 0.000 claims abstract description 13
- 238000004166 bioassay Methods 0.000 claims abstract description 9
- 238000009395 breeding Methods 0.000 claims abstract description 9
- 230000001488 breeding effect Effects 0.000 claims abstract description 9
- 235000021186 dishes Nutrition 0.000 claims abstract description 9
- 241000196324 Embryophyta Species 0.000 claims abstract description 8
- 239000000872 buffer Substances 0.000 claims abstract description 4
- 241001439019 Phthorimaea operculella Species 0.000 claims description 10
- 240000008042 Zea mays Species 0.000 claims description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- 230000003203 everyday effect Effects 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000012447 hatching Effects 0.000 claims description 2
- 231100000518 lethal Toxicity 0.000 claims description 2
- 230000001665 lethal effect Effects 0.000 claims description 2
- 101000693688 Spodoptera frugiperda Allatostatin Proteins 0.000 claims 2
- 241000607479 Yersinia pestis Species 0.000 description 4
- 241001057636 Dracaena deremensis Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241000255777 Lepidoptera Species 0.000 description 2
- 241001442207 Monochamus alternatus Species 0.000 description 2
- 101000693622 Spodoptera frugiperda Allatotropin Proteins 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 230000001617 migratory effect Effects 0.000 description 2
- 230000003071 parasitic effect Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 241000254173 Coleoptera Species 0.000 description 1
- 241000353522 Earias insulana Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 241000238704 Pyemotidae Species 0.000 description 1
- 241000256248 Spodoptera Species 0.000 description 1
- 241001454294 Tetranychus Species 0.000 description 1
- 241000132125 Tyrophagus Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Images
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M99/00—Subject matter not provided for in other groups of this subclass
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for preventing and controlling Spodoptera frugiperda, which comprises the following steps: step one, breeding pyemotes zhonghuajia by using aged larvae of tuber moths of potatoes, placing the aged larvae of the tuber moths of the potatoes parasitized with the pyemotes zhonghuajia into culture dishes after the parasitization of the pyemotes zhonghua is finished, wherein each culture dish contains the aged larvae of the tuber moths of potatoes until the dilatant body of the pyemotes zhonghuajia grows to 7-8 days old; step two, flatly placing a culture dish containing a 7-8 day old pyemotes zhonghuajia puffer body to the apical leaf heart or serious damage position of a plant damaged by spodoptera frugiperda; step three, after the pyemotes zhonghuajia puffiness grows mature, new pyemotes zhonghuajia comes out from the puffy bodies, and the pyemotes zhonghuajia can automatically search eggs and larvae of spodoptera frugiperda on the whole plant and parasitize the eggs and larvae to death; and step four, performing biological assay on spodoptera frugiperda larvae and spodoptera frugiperda eggs. The invention can well prevent and control Spodoptera frugiperda larvae in hidden positions, and is green and pollution-free.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to a Spodoptera frugiperda control method.
Background
Spodoptera frugiperda (Smith) belongs to Lepidoptera lepidoptera, noctudiae in Spodoptera, is a species of host, and is a typical migratory pest. The problem of drug resistance faced in the field of control of spodoptera frugiperda at present. Biological control is a typical migratory pest biological control, which is one of important measures in green prevention and control means of pests, and natural enemy insects have important regulation and natural control functions on the population occurrence of the pests.
Pyemotes zhonghuaajia belongs to Pyemotidae, has the characteristics of small size, quick movement, high toxicity, high male-female ratio and the like, belongs to an ectoparasitic natural enemy, and is originally used for preventing and controlling forestry stem borers such as monochamus alternatus, monochamus alternatus and the like. The body length of the adult is about 200 mu generally, the abdomen of the female pyemotes sinensis can expand by several times after parasitization, 250-350 progenies can be produced after 7-8 days of expansion, and the female percentage of the progenies reaches 95-99%. The pyemotes zhonghuajia can kill hosts 640000 times heavier than the pyemotes zhonghuajia and has better prevention and control effects on coleoptera, hymenoptera and lepidoptera insects.
The tuber moth is a substitute host with good effect for the pyemotes zhonghuajia, and the number of the pyemotes zhonghuajia which can parasitize and complete the expansion and development on the body surface of the mature larva of the tuber moth can reach about 150.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a method for preventing spodoptera frugiperda.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a Spodoptera frugiperda control method comprises the following steps:
step one, breeding pyemotes zhonghuajia by using aged larvae of tuber moths of potatoes, placing the aged larvae of the tuber moths of the potatoes, which are parasitized with the pyemotes zhonghuajia, in culture dishes after the pyemotes are parasitized, wherein each culture dish contains 4 to 5 aged larvae of the tuber moths of the potatoes until the bodies of the pyemotes zhonghuajia grow to 7 to 8 days old;
step two, flatly placing the culture dish containing the 7-8 day old pyemotes zhonghuajia puffer bodies in the step one to the top leaf heart of the plant endangered by spodoptera frugiperda or the serious damage part;
step three, after the pyemotes zhonghuajia grows to be mature, new pyemotes zhonghuajia comes out from the broken body, and the pyemotes zhonghuajia can automatically search eggs and larvae of spodoptera frugiperda on the whole plant and parasitize the eggs and the larvae to be lethal;
and step four, performing biological assay on spodoptera frugiperda larvae by using pyemotes zhonghuajia.
Further, in the fourth step, the spodoptera frugiperda larvae are subjected to bioassay by using the pyemotes zhonghuajia, and the specific process is as follows:
and 3, dripping water every day to keep moisture on the filter paper, recording the death condition of the larvae, and continuously recording the time of one week.
Further, in the fourth step, the pyemotes zhonghuajia is used for carrying out bioassay on spodoptera frugiperda eggs, and the specific process is as follows:
and 3, step 3: daily water drops were kept moist on filter paper while egg death and hatching was recorded for a period of one week.
Has the beneficial effects that: the invention utilizes the pyemotes zhonghuajia to prevent and control the field spodoptera frugiperda, is a green pollution-free biological control method, can well solve the problem of drug resistance in the field of preventing and controlling the spodoptera frugiperda at present, and can well prevent and control the spodoptera frugiperda larvae in hidden positions due to the characteristics of small size, quick movement and strong toxicity of the pyemotes zhonghuajia, thereby achieving the effect which cannot be achieved by the conventional control method.
Drawings
FIG. 1 is a schematic view of the structure of a culture dish according to an embodiment of the present invention; .
In the figure, 1 culture dish cover, 2 dish body, 3 buckle structure and 4 small hole.
Detailed Description
The invention is illustrated below with reference to specific examples. It will be understood by those skilled in the art that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention in any way.
A spodoptera frugiperda control method comprises the following steps:
step one, breeding pyemotes zhonghuajia by using aged larvae of tuber moths of potatoes, placing the aged larvae of the tuber moths of the potatoes parasitized with the pyemotes zhonghuajia into culture dishes after the parasitization of the pyemotes zhonghua is finished, wherein each culture dish contains 4 to 5 aged larvae of the tuber moths of potatoes until the puffiness of the pyemotes zhonghuajia grow to 7 to 8 days old;
step two, flatly placing the culture dish containing the 7-8 day old pyemotes zhonghuajia puffer bodies in the step one to the top leaf heart of the plant endangered by spodoptera frugiperda or the serious damage part;
step three, after the pyemotes zhonghuajia puffiness grows mature, new pyemotes zhonghuajia comes out from the puffy bodies, and the pyemotes zhonghuajia can automatically search eggs and larvae of spodoptera frugiperda on the whole plant and parasitize the eggs and larvae to death;
and step four, carrying out biological assay on spodoptera frugiperda larvae by using the pyemotes zhonghuajia.
Example 1: bioassay of Tetranychus zhonghuajia Tyrophagus hubner larvae
In an artificial climate room with the temperature of 25 +/-1 ℃ and the humidity of 60 +/-5%, feeding Spodoptera frugiperda larvae to 1, 2, 3, 4, 5 and 6 instars by using fresh corn leaves in a culture dish with the diameter of 9cm and containing filter paper respectively, wherein the number of the larvae in each instar is 20, and three groups of the larvae in each instar are added, and sterile water is dripped on the filter paper in the culture dish every day to keep the humidity in the culture dish. In a special culture dish paved with filter paper and 5cm in diameter, breeding pyemotes zhonghuajia with aged larvae of tuber moths, after parasitic pyemotes puffiness reach 7-8 days old, artificially selecting, respectively inoculating the tuber moths with the densities of 5, 10 and 15 puffiness into three groups of culture dishes for feeding larvae at all ages, recording the death condition of the larvae at all ages of spodoptera frugiperda every day, slightly touching the larvae with a writing brush to death, observing for 5 days totally, and counting the mortality of the spodoptera frugiperda.
Table 1 shows the mortality in 5 days (%)
Example 2: bioassay of pyemotes zhonghuajia against spodoptera frugiperda eggs
100 Spodoptera frugiperda eggs were placed on filter paper in a petri dish containing filter paper and having a diameter of 9cm in an artificial climate chamber having a temperature of 25 + -1 deg.C and a humidity of 60% + -5%. In a special culture dish paved with filter paper and having a diameter of 5cm, breeding pyemotes zhonghuajia with aged larvae of tuber moths, after parasitic pyemotes puffiness bodies reach 7-8 days old, artificially selecting, inoculating the tuber moths with the densities of 5, 10 and 15 puffiness bodies into the culture dish placed with 100 spodoptera frugiperda eggs, observing the death condition of the spodoptera frugiperda eggs recorded every day, and changing the color and damaging the eggs to death.
Table 2 shows the egg death after 5 days.
| 5 puffy bodies | 10 puffy bodies | 15 puffy bodies | |
| Death rate of ovum | 54% | 89% | 94% |
Example 3: control of spodoptera frugiperda larvae on corn by pyemotes zhonghuajia
Sowing corn seeds in a flowerpot with the caliber of 20cm, releasing Spodoptera frugiperda larvae of 2 years raised under the conditions that the temperature is 25 +/-1 ℃ and the humidity is 60 +/-5% onto corn leaves when the corn grows to a small trumpet-shaped period, releasing 20 young larvae from each corn plant, and starting the test after the corn plants are placed for 24 hours. In a special culture dish paved with filter paper and having a diameter of 5cm, breeding the pyemotes zhonghuajia with aged larvae of the tuber moths of potatoes, placing the tuber moths parasitized with the pyemotes zhonghuajia on the special culture dish according to the density of 1, 3 and 5 heads after the parasitized pyemotes puffiness reaches 7-8 days old, and then respectively placing on the heart leaves at the top ends of three corn plants. The death of Spodoptera frugiperda on maize plants was observed daily for 5 days.
Table 3 shows the spodoptera frugiperda larvae death status.
| 1 head of potato tuber moth | 3-headed potato tuber moth | 5-headed potato tuber moth | |
| Mortality of larvae | 45% | 80% | 95% |
The culture dish comprises a culture dish cover 1 and a dish body 2, wherein a buckle structure 3 is arranged between the culture dish cover 1 and the dish body 2 and is fastened or loosened according to requirements; the whole culture dish is a cylinder with the diameter of 5cm and the height of 1cm, and small holes 4 with the aperture of 500 mu m are uniformly distributed on the side surface of the culture dish.
Claims (4)
1. A Spodoptera frugiperda control method is characterized by comprising the following steps:
step one, breeding pyemotes zhonghuajia by using aged larvae of tuber moths of potatoes, placing the aged larvae of the tuber moths of the potatoes parasitized with the pyemotes zhonghuajia into culture dishes after the parasitization of the pyemotes zhonghua is finished, wherein each culture dish contains 4 to 5 aged larvae of the tuber moths of potatoes until the puffiness of the pyemotes zhonghuajia grow to 7 to 8 days old;
step two, flatly placing the culture dish containing the 7-8 day old pyemotes zhonghuajia puffer bodies in the step one to the top leaf heart of the plant endangered by spodoptera frugiperda or the serious damage part;
step three, after the pyemotes zhonghuajia grows to be mature, new pyemotes zhonghuajia comes out from the broken body, and the pyemotes zhonghuajia can automatically search eggs and larvae of spodoptera frugiperda on the whole plant and parasitize the eggs and the larvae to be lethal;
and step four, performing biological assay on spodoptera frugiperda larvae and spodoptera frugiperda eggs by using pyemotes zhonghuajia.
2. The method for controlling spodoptera frugiperda as claimed in claim 1, wherein in the fourth step, the spodoptera frugiperda larvae are bioassayed by pyemotes zhonghuanii, and the specific process is as follows:
step 1, feeding Spodoptera frugiperda larvae to 1, 2, 3, 4, 5 and 6 instars by using corn leaves in a culture dish containing filter paper under the environment that the temperature is 25 +/-1 ℃ and the humidity is 60% +/-5%, wherein 20 larvae are grown in each instar, and three groups are formed in each instar;
step 2, artificially treating the aged potato tuber moth larvae parasitized with 7-8 days old pyemotes zhonghuajia in the step one, and respectively inoculating the aged potato tuber moth larvae with the density of 5, 10 and 15 puffiness into three culture dishes for breeding 1, 2, 3, 4, 5 and 6 days old spodoptera frugiperda larvae;
and 3, dripping water every day to keep moisture on the filter paper, recording the death condition of the larvae, and continuously recording the time of one week.
3. The method for controlling spodoptera frugiperda as claimed in claim 1, wherein in the fourth step, the pyemotes zhonghuanii is used for performing bioassay on spodoptera frugiperda eggs, and the specific process is as follows:
step 1, placing 100 spodoptera frugiperda eggs in a culture dish containing filter paper in an environment with the temperature of 25 +/-1 ℃ and the humidity of 60% +/-5%, and combining 3 groups;
step 2, artificially treating the aged potato tuber moth larvae parasitized with 7-8 days old pyemotes zhonghuajia in the step one, and respectively inoculating the density of 5, 10 and 15 puffiness into three groups of culture dishes placed with ova spodoptera frugiperda eggs;
and 3, step 3: the eggs were kept moist on filter paper by dripping water every day while their death and hatching was recorded for a period of one week.
4. The spodoptera frugiperda control method as claimed in any of claims 1 to 3, wherein the culture dish comprises a culture dish cover and a dish body, a buckle structure is arranged between the culture dish cover and the dish body, and the culture dish cover and the dish body are fastened or loosened according to requirements; the whole culture dish is a cylinder with the diameter of 5cm and the height of 1cm, and small holes with the aperture of 500 mu m are uniformly distributed on the side surface of the culture dish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211014475.7A CN115316347A (en) | 2022-08-23 | 2022-08-23 | Spodoptera frugiperda control method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211014475.7A CN115316347A (en) | 2022-08-23 | 2022-08-23 | Spodoptera frugiperda control method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569297A (en) * | 2009-06-08 | 2009-11-04 | 河北省农林科学院昌黎果树研究所 | Breeding method of pyemotes zhonghuajia and application thereof in pest biological control |
| US20160100583A1 (en) * | 2013-05-17 | 2016-04-14 | Lee Antimicrobial Solutions, Llc | Methods for the Control of Arthropods Using Near-Ideal Gas Phase Hydrogen Peroxide |
| CN114403097A (en) * | 2021-11-19 | 2022-04-29 | 贵州大学 | Breeding device and breeding method for pyemotes zhonghuajia |
-
2022
- 2022-08-23 CN CN202211014475.7A patent/CN115316347A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569297A (en) * | 2009-06-08 | 2009-11-04 | 河北省农林科学院昌黎果树研究所 | Breeding method of pyemotes zhonghuajia and application thereof in pest biological control |
| US20160100583A1 (en) * | 2013-05-17 | 2016-04-14 | Lee Antimicrobial Solutions, Llc | Methods for the Control of Arthropods Using Near-Ideal Gas Phase Hydrogen Peroxide |
| CN114403097A (en) * | 2021-11-19 | 2022-04-29 | 贵州大学 | Breeding device and breeding method for pyemotes zhonghuajia |
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