CN115212230A - 含有干细胞胞外囊泡的药物组合物及其在呼吸道炎症治疗中的应用 - Google Patents
含有干细胞胞外囊泡的药物组合物及其在呼吸道炎症治疗中的应用 Download PDFInfo
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PCT/CN2022/088118 WO2022222987A1 (zh) | 2021-04-21 | 2022-04-21 | 含有干细胞胞外囊泡的药物组合物及其在呼吸道炎症治疗中的应用 |
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---|---|---|---|---|
US20150190429A1 (en) * | 2012-07-18 | 2015-07-09 | Universitaet Duisburg-Essen | Use of Preparations Comprising Exosomes Derived From Mesenchymal Stem Cells (MSCs) in the Prevention and Therapy of Inflammatory Conditions |
CN105209881A (zh) * | 2013-03-13 | 2015-12-30 | 迈阿密大学 | 从细胞培养上清液和生物流体分离并纯化微泡的方法 |
US20180042847A1 (en) * | 2015-03-16 | 2018-02-15 | Duncan ROSS | Method of treatment comprising membrane-enclosed vesicle |
CN113384597A (zh) * | 2020-03-13 | 2021-09-14 | 西比曼生物科技(上海)有限公司 | 含人体细胞衍生的细胞膜外囊泡的雾化吸入制剂、制法及其应用 |
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MX2021001574A (es) * | 2018-08-10 | 2021-07-15 | Omnispirant Ltd | Vesiculas extracelulares para inhalacion. |
CN112538456B (zh) * | 2019-09-20 | 2024-05-07 | 北京干细胞与再生医学研究院 | 一种多能干细胞、药物组合物及其制备方法与用途 |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150190429A1 (en) * | 2012-07-18 | 2015-07-09 | Universitaet Duisburg-Essen | Use of Preparations Comprising Exosomes Derived From Mesenchymal Stem Cells (MSCs) in the Prevention and Therapy of Inflammatory Conditions |
CN105209881A (zh) * | 2013-03-13 | 2015-12-30 | 迈阿密大学 | 从细胞培养上清液和生物流体分离并纯化微泡的方法 |
US20180042847A1 (en) * | 2015-03-16 | 2018-02-15 | Duncan ROSS | Method of treatment comprising membrane-enclosed vesicle |
CN113384597A (zh) * | 2020-03-13 | 2021-09-14 | 西比曼生物科技(上海)有限公司 | 含人体细胞衍生的细胞膜外囊泡的雾化吸入制剂、制法及其应用 |
Non-Patent Citations (2)
Title |
---|
MARK A RIDER等: "ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles", 《SCIENTIFIC REPORTS》, vol. 6, pages 1 - 14 * |
熊文杰 等: "超速离心法与聚乙二醇沉淀法提取人多发性骨髓瘤细胞来源外泌体的比较", 《深圳中西医结合杂志》, vol. 28, no. 2, pages 6 - 8 * |
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Address after: North Zone, 1st Floor, Building 1, No. 159 Cailun Road, China (Shanghai) Pilot Free Trade Zone, Pudong New Area, Shanghai, 201210 Applicant after: Shibiman Biotechnology (Shanghai) Co.,Ltd. Address before: North Zone, 1st Floor, Building 1, No. 159 Cailun Road, China (Shanghai) Pilot Free Trade Zone, Pudong New Area, Shanghai, 201210 Applicant before: Shibiman Biotechnology (Shanghai) Co.,Ltd. |
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