CN115197951B - 茶树黄酮醇类合成候选基因CsNAC086及其应用 - Google Patents
茶树黄酮醇类合成候选基因CsNAC086及其应用 Download PDFInfo
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Abstract
本发明公开了一种茶树NAC转录因子CsNAC086及其在调控黄酮醇类化合物代谢中的应用，所述茶树NAC转录因子CsNAC086的核苷酸序列如SEQ ID NO：1所示。在烟草中过量表达CsNAC086基因后，烟草花中的黄酮醇含量显著提高。
Description
技术领域
本发明涉及植物基因工程领域，具体涉及一种茶树NAC转录因子CsNAC086及其在调控黄酮醇类化合物代谢中的应用。
背景技术
茶兼具独特的风味口感与保健功效，是当今世界上最畅销的非酒精饮品之一，也是我国农产品中重要的农业作物与文化输出的产品。高黄酮醇含量的茶树鲜叶能为高品质茶叶的生产提供基础，并为饮茶者带来多重的保健功效。因此，调控黄酮醇合成基因的挖掘对于茶树新品种的选育也具有重要的意义。
黄酮醇属于黄酮类化合物，是黄酮类化合物中数量最多，分布最广的一类。常见的有山奈酚、槲皮素、杨梅素、异鼠李素和芦丁5种。普遍存在于植物的根、茎、叶、花、果实、种子中，具有药用、抗氧化、调节花色、调控激素、抗紫外线和抗病虫害等作用。黄酮醇在植物抗干旱、盐胁迫、低温、紫外线胁迫和植物激素胁迫等非生物胁迫中也具有重要作用。
黄酮醇类有多种生理活性，包括抗氧化、抗菌、抗炎、防癌、抗病毒和预防心血管疾病（Kumar and Pandey, 2013）。流行病学调查显示，黄酮醇类能降低罹患癌症的风险（Calderon-Montano et al., 2011 , Wang et al., 2016），其中槲皮素能使增殖的淋巴样细胞产生周期停滞，并可抑制多种恶性肿瘤细胞生长（Lamson and Brignall, 2000）。槲皮素对狂犬病病毒和脊髓灰质炎病毒有抗性，芦丁则可抵御流感病毒和马铃薯病毒侵袭（Middleton, 1998）。而且黄酮醇与黄酮在抗病毒中具有强烈的协同增效作用。据报道，山奈酚和木犀草素复配可显著提升其对单纯疱疹病毒（HSV）的防效，槲皮素还可增强 5-乙基-2-二氧尿苷和阿昔洛韦对 HSV和假狂犬病毒的治疗效果（Cushnie and Lamb, 2005）。
黄酮醇类是茶树中常见的黄酮类物质，约占到茶叶干重的3%~4%（宛晓春, 2003）。黄酮醇极易溶于水，呈现出黄绿色，被认为是形成绿茶汤色的主要成分。且该类物质易发生自动氧化，是多酚类化合物自动氧化的主要物质，从而导致绿茶汤色劣变，对茶饮料品质影响极大。研究表明，黄酮醇类不仅是茶汤中主要的涩味成分，还可以明显加强咖啡因的苦味（Scharbert and Hofmann, 2005）。
植物组织内黄酮醇的合成与积累受到植物种类、发育阶段、组织部位以及生长环境等多方因素的协同调控，且黄酮醇合成酶（FLS）、查尔酮合成酶（CHS）、查尔酮异构酶（CHI）等关键酶基因的表达水平在调控黄酮醇的生物合成和组织积累中发挥重要作用。研究显示，黄酮类化合物的合成途径在转录水平上主要受R2R3-MYB转录因子、bHLH转录因子和WD40蛋白的调控。MYB、bHLH和WD40转录因子单独或形成复合体后调节CHS、CHI和FLS等关键酶基因的表达，进而调控黄酮醇的生物合成。其他类转录因子，如NAC、WRKY和bZIP等家族部分成员也被证实在转录水平上调控黄酮醇生物合成关键基因表达进而影响植物黄酮醇的合成。从挪威云杉中鉴定的NAC家族转录因子PaNAC03通过负调控CHS、F3'H和PaLAR3的表达，抑制黄酮类化合物的合成（Dalman et al., 2017）。在拟南芥中的研究发现，NAC转录因子ANAC078在高光条件下促进植物类黄酮合成途径中相关基因的转录（Morishita et al.,2009）。虽然对于NAC转录因子的研究随着生物技术的进步已经有了许多成果，但是相比于MYB、bHLH和WD40等类型的转录因子来说，NAC转录因子仍有很大的探索空间。截止目前，茶树中NAC转录因子调控黄酮醇类化合物合成的相关机理尚不明确，有待于进一步研究。
发明内容
为了克服现有技术的上述缺点与不足，本发明的首要目的在于提供一种茶树NAC转录因子CsNAC086基因。
本发明的另一个目的在于提供上述茶树NAC转录因子CsNAC086蛋白。
本发明的另一个目的在于提供一种含有上述茶树NAC转录因子CsNAC086的重组表达载体。
本发明的另一个目的在于提供一种含有上述茶树NAC转录因子CsNAC086的转基因植株。
本发明上述目的是通过以下技术方案来实现的
第一方面，本发明提供一种茶树NAC转录因子，所述茶树NAC转录因子的氨基酸序列如SEQ ID NO：2所示。
第二方面，本发明提供一种上述茶树NAC转录因子的编码基因。
所述编码基因可以针对不同用途进行不同的优化，只要其编码的氨基酸序列与本发明相同即在本发明的保护范围内。本发明的一个实施例中所述茶树NAC转录因子的编码基因的核苷酸序列如SEQ ID NO：1所示。
第三方面，本发明提供一种上述编码基因构建的重组质粒。
进一步，所述重组质粒的载体为pCAMBIA2300载体。
具体地，所述重组质粒按如下方法构建：
（1）以从茶树‘紫娟’（Camellia sinensis CV. Zijuan）新梢叶片提取的总RNA为模板，反转录得到cDNA，以所述cDNA为模板，通过以下引物进行PCR扩增得到茶树NAC转录因子的编码基因：pCAMBIA2300-CsNAC086-F：5’-ATGGCACCTGTTTCGTTGCC-3’，
pCAMBIA2300-CsNAC086-R：5’-CTATGGGGAATTAGGTGCAATATGC-3’。
（2）用限制性内切酶BamH I与Sal I对 pCAMBIA2300载体进行双酶切，得到线性化载体；以ClonExpress ® II One Step Cloning Kit对步骤（1）所述茶树NAC转录因子的编码基因和所述线性化载体进行一步克隆，以所得一步克隆的产物转化感受态细胞，经含卡那霉素的培养基筛选和测序验证后，挑选阳性克隆培养并提取质粒，即得到所述重组质粒。
第四方面，本发明提供一种上述重组质粒转化宿主菌制备的重组基因工程菌。进一步，所述宿主菌为农杆菌。
优选地，所述重组基因工程菌按如下方法构建：热激法将所述重组质粒转入农杆菌GV3101感受态细胞，经含所述重组质粒的载体所携带的抗性基因指向的抗生素的YEB固体培养基筛选，菌落PCR检验，得到所述重组基因工程菌。
具体地，所述重组质粒所携带的抗性基因为卡那霉素和利福平抗性；所述重组基因工程菌按如下方法构建：
取-80℃保存的100 μL农杆菌GV3101感受态细胞置冰上，完全解冻后轻轻将细胞悬浮；加入5 μL所述重组质粒，混匀后冰上放置30 min；放入液氮中速冻5 min，37℃水浴中热激1 min，迅速转移至冰上，冰浴2 min；加入1 mL YEB液体培养基，28℃振荡培养1 hr；4000 rpm离心5 min，加100 μL YEB培养液悬浮细胞；将所得稀释菌液涂布于含50 mg/L 卡那霉素和100 mg/L利福平的YEB平板培养基上，28℃培养32～48 hr；挑取单克隆菌落接种于含50 mg/L 卡那霉素和50 mg/L利福平的YEB液体培养基中28℃培养24 hr，再通过PCR对菌落进行检测，筛选阳性克隆，即所述重组基因工程菌。
第五方面，本发明提供一种上述重组基因工程菌在构建过表达黄酮醇的植株中的应用。
优选地，所述植株为烟草；所述重组基因工程菌的宿主菌为农杆菌。
具体地，所述重组基因工程菌所含重组质粒的载体为pCAMBIA2300载体，宿主菌为农杆菌；所述过表达黄酮醇的植株按如下方法构建：
将本氏烟草（Nicotiana benthamiana）无菌苗上部完全展开的幼嫩叶片裁剪成小块浸泡于所述重组基因工程菌的菌液中10 min，所述重组基因工程菌的菌液的OD600为0.6；取出叶片后，转移无菌滤纸上吸干液体，然后于共培养培养基上28℃暗培养三天；然后转入出芽选择培养基中在28℃的培养温度下按照光照16 hr/d（所述光照的强度为1000~1500lx），黑暗8 hr/d进行培养，进行抗性芽的筛选；切下所得抗性芽转入壮苗选择培养基，在28℃的培养温度下按照光照16 hr/d（所述光照的强度为1000~1500 lx），黑暗8 hr/d进行生根培养，即得到所述过表达黄酮醇的植株。
进一步，所述共培养培养基为MS培养基，所述共培养培养基的终浓度组成如下：2.25 mg/L 6-苄氨基嘌呤，0.3 mg/L萘乙酸，30.0g/L蔗糖，8.0g/L琼脂，溶剂为水，pH6.0。
进一步，所述出芽选择培养基为MS固体培养基，所述出芽选择培养基的终浓度组成如下：2.25 mg/L 6-苄氨基嘌呤，0.3 mg/L萘乙酸，100 mg/L卡纳抗生素，400 mg/L头孢噻吩(CEF)，30.0 g/L蔗糖，8.0 g/L琼脂，溶剂为水，pH 6.0。
进一步，所述的壮苗选择培养基为MS固体培养基，所述的壮苗选择培养基的终浓度组成如下： 0.1 mg/L 6-苄氨基嘌呤，0.01 mg/L萘乙酸，100 mg/L卡那霉素，400 mg/L头孢噻吩(CEF)，30.0 g/L蔗糖，8.0 g/L琼脂，溶剂为水，pH 6.0。上述得到的是幼苗，按如下方法进行移栽：洗掉转基因烟草植株根上的残留培养基，将具有良好根系的幼苗转入温室，同时在培养的第一周保证土壤有充足的水分。
与现有技术相比，本发明具有如下有益效果：
本发明获得一种茶树NAC转录因子CsNAC086的核苷酸序列，并对其氨基酸序列进行分析，发现CsNAC086具有NAC转录因子的保守结构域。CsNAC086连接到具有35S启动子的过表达载体pCAMBIA2300上，利用农杆菌GV3101介导转化烟草，通过HPLC检测到过表达植株烟草花瓣的黄酮醇类化合物含量显著提高。附图说明
图1为其他物种中的NAC转录因子和CsNAC086的氨基酸序列分析图。
图2为野生型烟草花瓣和过量表达CsNAC86基因烟草花瓣的黄酮醇含量图。
图3为野生型烟草花瓣和过量表达CsNAC086基因烟草花瓣的黄酮醇HPLC图。
图4为黄酮醇合成酶基因FLS在野生型以及过表达CsNAC086基因烟草中的表达量情况图。
图5为CsNAC086调控黄酮醇合成通路的模拟示意图。
实施方式
下面结合说明书附图和具体实施例对本发明作出进一步地详细阐述，所述实施例
只用于解释本发明，并非用于限定本发明的范围。下述实施例中所使用的试验方法如无特
殊说明，均为常规方法；所使用的材料、试剂等，如无特殊说明，均为可从商业途径得到的试剂和材料。
（1）Yeast Extract Mannitol Broth （YEB）液体培养基：10 g胰蛋白胨，10 g酵母提取物，5 g NaCl，加蒸馏水定容至1 L，分装后121℃高压灭菌15~20 min；
（2）YEB固体培养基：每升YEB液体培养基加15 g琼脂粉；
（3）YEB固体选择培养基：待YEB液体培养基温度降至50℃左右时加入50 mg/L 卡纳抗生素；
（4）Murashige and Skoog Stock （MS）液体培养基：称取4.43 g MS培养基粉剂（不含琼脂）加蒸馏水定容至1 L，用NaOH调PH至5.8，121℃高压灭菌15 min；
（5）MS固体培养基：称取4.43 g MS培养基粉剂（不含琼脂）加8 g琼脂粉，加蒸馏水定容至1 L，用NaOH调PH至5.8，121℃高压灭菌15 min；
（6）共培养培养基：MS固体培养基，2.25 mg/L 6-苄氨基嘌呤，0.3 mg/L 萘乙酸，30.0g/L 蔗糖，8.0g/L琼脂，加蒸馏水定容至1L，pH调至6.0。
（7）出芽选择培养基：MS固体培养基，2.25 mg/L 6-苄氨基嘌呤，0.3 mg/L萘乙酸，100 mg/L卡纳抗生素，400 mg/L 头孢噻吩(CEF)，30.0 g/L蔗糖，8.0 g/L琼脂，加蒸馏水定容至1L，pH调至6.0。
（8）壮苗选择培养基：MS固体培养基，0.1 mg/L 6-苄氨基嘌呤，0.01 mg/L萘乙酸，100 mg/L 卡纳抗生素，400 mg/L 头孢噻吩(CEF)，30.0 g/L蔗糖，8.0 g/L琼脂，加蒸馏水定容至1L，pH调至6.0。
（9）生根选择培养基：MS固体培养基，100 mg/L 卡纳抗生素，400 mg/L头孢噻吩(CEF)，30.0 g/L蔗糖，8.0 g/L琼脂，加蒸馏水定容至1L，pH调至6.0。实施例1茶树NAC转录因子CsNAC086基因的克隆和过表达载体的构建
1、RNA的提取：使用天根生化科技（北京）有限公司的RNA prep Pure多糖多酚植物总RNA提取试剂盒以‘紫娟’茶树新梢叶片为材料提取总RNA，使用琼脂糖凝胶电泳分析检测RNA的完整性，使用Nanodrop检测RNA的浓度（OD260/OD280读数在1.8~2.1之间为高质量RNA），剩余RNA在-80℃保存备用。
2、 cDNA第一链的合成：使用莫纳生物科技有限公司的MonScript™ RTIII SuperMix with dsDNase反转录试剂盒合成cDNA第一条链。在高压灭菌过的RNase-Free离心管中依次加入反应试剂（表1），37 ℃恒温反应2 min去除RNA中的DNA。将去除DNA的反应产物，加入第一链cDNA合成试剂（表2）。在50℃恒温反应15 min，反应结束后，85℃温育5 min以终止反应，获得cDNA溶液。贮存于-20℃冰箱备用。
3、 CsNAC086过表达载体的构建：
根据CsNAC086的基因序列设计连接pCAMBIA2300载体的引物，pCAMBIA2300-CsNAC086-F：5’-ATGGCACCTGTTTCGTTGCC-3’，
pCAMBIA2300-CsNAC086-R：5’-CTATGGGGAATTAGGTGCAATATGC-3’，以‘紫娟’茶树cDNA为模板，进行PCR扩增。PCR扩增：反应条件：94℃预变性5 min；94℃ 20 s，58℃ 30 s，72℃ 2 min 30 s，30个循环；72℃延伸7 min。扩增得到CsNAC086基因。
用BamH I（TaKaRa）与Sal I（TaKaRa）双酶切pCAMBIA2300载体使其线性化后，采用ClonExpress ® II One Step Cloning Kit（南京诺唯赞生物科技有限公司）将扩增得到的基因和线性化的pCAMBIA2300载体进行连接。酶切体系：BamH I 1μL+Sal I 1μL+10×Buffer 2 μL+质粒3μL+ H2O补齐到10 μL，37℃ 温育2 hr，琼脂糖凝胶电泳分析酶切结果后回收。将所得回收产物和连接试剂混匀（表3），置于37℃反应30 min。待反应完成后，立即将反应管置于冰水浴中冷却5 min，待转化。
2、大肠杆菌感受态的制备
（1）从-80℃冰箱中取出保存的DH5α接种在YEB固体培养基上，37℃过夜培养（大约12 h）；
（2）挑取YEB固体培养基上的DH5α单菌落，接种到3~5 mL不含抗生素的 YEB液体培养基中，37℃过夜震荡培养（大约12 h）；
（3）将该菌液以1：100接种，37℃震荡培养2~3 hr至OD600=0.5；
（4）将菌液移入50 mL离心管中，在冰上放置10 min；
（5）4℃，4000 r/min离心10 min，弃上清液；
（6）用预冷的0.1 mol/L的CaCl2溶液将菌体轻轻悬浮，冰上放置30 min；
（7）4℃，4000 r/min离心10 min，弃上清液，加入2 mL预冷的0.1 mol/L的CaCl2溶液，轻轻重悬菌液，既制成感受态细胞；
（8）将制备好的感受态细胞菌液与30%甘油1:1混合（甘油终浓度为15%）；
（9）将感受态细胞分装为100 μL/管，液氮速冻后放入-80℃冰箱保存。
3、重组质粒转化大肠杆菌感受态以及阳性克隆鉴定
（1）在冰上融化大肠杆菌感受态细胞，取10 μL重组产物加入100 μL感受态细胞中，吸打混匀（避免剧烈震荡），冰上放置30 min；
（2）置于42℃水浴锅热激80 s后，立即置于冰上放置3~5 min；
（3）加入700 μL YEB液体培养基（不含任何抗生素），37℃ 200 r/min摇菌1 hr；
（4）将菌液室温5,000 r/min 离心5 min，弃700 μL上清液，用管中剩余培养基将菌块重新悬浮，涂布到含有卡纳抗生素（50 mg/L）的YEB固体培养基上；
（5）37℃培养12~16 hr；
（6）挑取单克隆，使用至少一条载体上的通用引物和克隆基因的特异性引物进行PCR扩增，按照PCR反应体系（表4）使用北京擎科新业生物技术有限公司的T5 DNA聚合酶进行阳性克隆的筛选。将阳性克隆的单菌落进行扩繁，送公司测序，序列比对正确后，提取质粒保存于-20℃备用。
将测序结果进行序列分析发现：CsNAC086的开放阅读框为2034个碱基，如SEQ IDNO：1所示，编码678个氨基酸（如SEQ ID NO：2所示），具有NAC转录因子的保守结构域（见图1）。
实施例2茶树NAC转录因子CsNAC086过表达烟草的转化
1、农杆菌感受态的制备
（1）挑取农杆菌（Agrobacterium tumefaciens）GV3101单菌落，接种于10 mL YEB培养基中，28℃振荡培养过夜；
（2）取400 μL菌液置于50 mL YEB培养液中，28℃振荡培养5～6 h至OD600为0.5；
（3）菌液倒入50 mL离心管中，6000 rpm 离心5 min 后，弃上清液；
（4）将菌体重悬于10 mL 0.15 mol/L的NaCl中，6000 rpm离心5 min，弃上清液；
（5）菌体用l mL预冷的20 mmol/L CaCl2溶液轻轻悬浮，加入预冷的15%甘油，100μL分装，-80℃冰箱保存备用。
2、重组质粒转化农杆菌感受态细胞
（1）取-80℃保存的100 μL农杆菌感受态细胞置冰上，完全解冻后轻轻将细胞悬浮；
（2）加入5 μL质粒混匀后冰上放置30 min；
（3）将离心管放入液氮中速冻5 min，37℃水浴中热激1 min，迅速转移至冰上，冰浴2 min；
（4）加入1 mL YEB液体培养基，28℃振荡培养1 hr；
（5）4000 rpm离心5 min，加100 μL YEB培养液悬浮细胞；
（6）将菌液涂布于含有卡纳抗生素（50 mg/L）和利福平抗生素（50 mg/L）的YEB平板上，28℃培养32～48 hr；
（7）挑取长出的农杆菌菌落于含有卡纳抗生素（50 mg/L）和利福平抗生素（50 mg/L）的YEB液体培养基28℃摇菌24 hr，再通过PCR对菌落进行检测筛选阳性克隆。
3、农杆菌介导的烟草遗传转化步骤
（1）农杆菌的培养
首先，在带有卡纳抗性的YEB固体培养基（10g/L蛋白胨 + 10g/L 酵母提取物 +5g/L氯化钠 + 50 mg/L 卡纳+ 15g/L琼脂）上28℃预培养GV3101农杆菌48h；挑取农杆菌单菌落，接种于卡纳抗性的YEB液体培养基（10g/L蛋白胨 + 10g/L 酵母提取物 + 5g/L氯化钠 + 50 mg/L 卡纳）中，于28℃，200 rpm震荡培养过夜，至菌液浓度OD600值大约为0.6。
（2）叶盘转化法转化烟草
a.剪取本氏烟草（Nicotiana benthamiana）无菌苗上部完全展开的幼嫩叶片，将叶片剪成0.8 cmÍ0.8 cm大小小块，放入无菌烧杯中；
b.将准备好的菌液倒入烧杯，轻轻摇晃烧杯。叶片在菌液中浸泡10 min；
c.将步骤b 中的叶片取出，转移至灭好菌的滤纸上吸干；然后放置在如上所述的共培养培养基上28℃暗培养三天；
d.三天后，将叶片转入如上所述的出芽选择培养基上，28℃光照和暗培养（光照强度1000-1500 lx，光照时间：16h/d，黑暗时间：8h/d）交替培养，进行卡纳抗性芽的筛选分化；
e.抗性芽形成后，将其切下，转入如上所述的壮苗选择培养基上，在28℃光照和暗培养（光照强度1000-1500 lx，光照时间：16h/d，黑暗时间：8h/d）交替培养，进行卡纳抗性苗的筛选；
f.将筛选得到的抗性苗转入如上所述的生根选择培养基上使其生根，在28℃光照和暗培养（光照强度1000-1500 lx， 光照时间：16h/d，黑暗时间：8h/d）交替培养。
（3）移栽
洗掉转基因本氏烟草植株根上的残留培养基，将具有良好根系的幼苗转入温室，同时在第一周保持土壤有充足的水分。
实施例3 NAC转录因子CsNAC086过表达烟草植株中黄酮醇含量的测定
1、分别取野生型和过表达CsNAC086的烟草花瓣0.2 g，在1 mL提取液（1%盐酸甲醇（v:v）盐酸浓度36%-38%）中均质，然后在4℃的黑暗环境中放置24 h。
2、使用TSK ODS-80Ts QA（4.6mm×250mm，5µm，Tosoh）柱通过HPLC分析黄酮醇的含量。流动像中溶剂A是10%的甲酸（水的体积分数），溶剂B是甲醇。梯度条件为：0 min，17%溶剂B；15 min，35%溶剂B；40 min，37%溶剂B；42 min，100%溶剂B；44 min，100%溶剂B；45 min，17%溶剂B；46 min，17%溶剂B。进样量为10 µL，色谱柱柱温为40℃，流速为1.0 mL/min，在360 nm处检测黄酮醇的吸收峰面积。黄酮醇以槲皮素-3-葡萄糖苷为标准品计算含量。测定结果如图2和图3。
实施例4黄酮醇合成酶基因FLS在野生型和CsNAC086过表达烟草中的表达分析
本实施例分析了黄酮醇合成酶基因FLS在野生型和过表达CsNAC086烟草中的表达情况。具体步骤为，根据FLS和内参基因Actin的序列设计引物（见表5），使用天根生化科技（北京）有限公司的RNA prep Pure多糖多酚植物总RNA提取试剂盒对烟草的叶片进行总RNA的提取，然后使用莫纳生物科技有限公司的MonScript™ RTIII Super Mix with dsDNase反转录试剂盒反转成cDNA，将cDNA稀释10倍作为qRT-PCR的模板。使用南京诺唯赞生物科技股份有限公司的ChamQ SYBR qPCR Master Mix进行qRT-PCR反应，每个反应重复3次。qRT-PCR反应体系如下：cDNA模板2 µL，2×SYBRGreenMIX 10 µL，10 µmol /L正向引物0.4 µL，10 μmol/ L反向引物0.4 µL，加水至总体积达到20 µL。qRT-PCR程序：95℃，10 min；95℃，10 s；58℃，10 s；72℃，15 s；35个循环；72℃，20 min。反应产物用融解曲线分析。使用耶拿分析仪器（北京）有限公司的实时荧光定量 PCR 仪收集数据，FLS基因的相对表达量用2-△△CT法计算，结果见图4。
序列表
<110> 浙江农林大学
<120> 茶树黄酮醇类合成候选基因CsNAC086及其应用
<160> 8
<170> SIPOSequenceListing 1.0
<210> 1
<211> 2034
<212> DNA
<213> 茶树(Camellia sinensis )
<400> 1
atggcacctg tttcgttgcc acctggtttc cggttccatc ctactgatga agaacttgtg 60
gcttactatc tcaagagaaa gattaatggg cgaaagatcg aactcgagat catccctgaa 120
gttgatctct acaagtgcga gccttgggac ttaccaggaa aatcattatt gccaagcaag 180
gatctagagt ggtacttctt tagtccacga gaccgcaaat acccaaatgg atcaaggaca 240
aatcgcgcaa ctaaagctgg gtactggaag gcgacaggga aggatcgaaa agtgaactca 300
cagatgaggg ctgtggggat gaagaaaacc ttagtttact acagagggag ggcaccgcat 360
ggcgctcgta ctgattgggt catgcatgag tacaggcttg atgagagaga atgtgaaact 420
ccctcaggct tgcaggatgc ttacgcgcta tgccgggtgt ttaagaagag tgcaacaggg 480
ccaaagatag gagagcatta tgtagggtca ggttcaaata ataaccagat gtctagtgat 540
cactcatcta gtatgaatga cctttattct gagggaagag gtgaggattt ggagagctat 600
gattataatc aaatggcatt gcatcacagt acaagctcac ccaacatgaa catccatgtt 660
ggatctccta cagtgaatac caataccagc agtgctgcaa ctgatgggaa ttggatgcgc 720
tacttatcgg aggatgcatt tagcttcacc aatccgtctt tttcaaattg cggaaacatt 780
tcctatcccc catccaaggt ggatatagca ttggagtgcg caaggttgca gcaccggctg 840
tcaatgccac cactggaggt gcatgacttc ccacagggtg gcttcgtcga tttaagaata 900
ccacagtccg attccatccg cagccacaac ccaaatgatc acgaagaaca cgacattttg 960
caggaaattc tgtctgtagc tcaggcttct caagaactga tcaatcaaga catgacatgg 1020
ggggttacta gtggtggtgg aagcttctat gcccaacaag ctggtgatga tttctctttt 1080
catcctcatg ctgtccaaat tcatgacatg atgaataatg gtggtggtgg tggttcttca 1140
agattgatta tggaagatca gcagcataca atctcaaggt ccatagagat tggggggttc 1200
gacaaagatt tcaaaactga aagaacggtg gagaacttgc gatgggttgg aatgtctagc 1260
aaagacctag agaagaactt ttcagaagat tacaagactg ttccaataga aaccatctcc 1320
tgtttccaga caagcgaaga ccaagaggtt cgaggagaaa ttgatcatca aagcaacttc 1380
aatgaattca atgacaggga agcgaatgac ttcccatttg gattcaccga cgataacctg 1440
aacgacaatt ttttagatga tagtcatgaa gttgatgact tttctagtac tccaaacttt 1500
gaagttcatg aaaaaattga ggtgagtcat ggactgttcg tctcaactcg ccaagtagct 1560
gagacattct ttcaccaaat agttccctca gagattgtca aggtctatct aaatccagtg 1620
ataacaacac atcactctcc aatgacaaaa ccagactcaa caacaaaacc caagaagcca 1680
tggaggaaaa tagtgagccc aagcatcact atcattgcac ttctattgac ttactattgc 1740
gtgtattttg gagaacactt ggaggatgag aagttgaggg acaactttgt agataacact 1800
agcaaaggtg tgaagaggag agagaaagat tgctctaatg atgatgaggt gaacaagatg 1860
aagaaacatg gtggaacatt aatgtggaag aattacaaga agaggaattg gtttgttggg 1920
agtataggag ggagcatatg tagtatggtt ttgaaccagg tttggcctta ccttactata 1980
gctttggctc tttgtagcat ttgggtgcac catattgcac ctaattcccc atag 2034
<210> 2
<211> 677
<212> PRT
<213> 茶树(Camellia sinensis )
<400> 2
Met Ala Pro Val Ser Leu Pro Pro Gly Phe Arg Phe His Pro Thr Asp
1 5 10 15
Glu Glu Leu Val Ala Tyr Tyr Leu Lys Arg Lys Ile Asn Gly Arg Lys
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Ile Glu Leu Glu Ile Ile Pro Glu Val Asp Leu Tyr Lys Cys Glu Pro
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Trp Asp Leu Pro Gly Lys Ser Leu Leu Pro Ser Lys Asp Leu Glu Trp
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Tyr Phe Phe Ser Pro Arg Asp Arg Lys Tyr Pro Asn Gly Ser Arg Thr
65 70 75 80
Asn Arg Ala Thr Lys Ala Gly Tyr Trp Lys Ala Thr Gly Lys Asp Arg
85 90 95
Lys Val Asn Ser Gln Met Arg Ala Val Gly Met Lys Lys Thr Leu Val
100 105 110
Tyr Tyr Arg Gly Arg Ala Pro His Gly Ala Arg Thr Asp Trp Val Met
115 120 125
His Glu Tyr Arg Leu Asp Glu Arg Glu Cys Glu Thr Pro Ser Gly Leu
130 135 140
Gln Asp Ala Tyr Ala Leu Cys Arg Val Phe Lys Lys Ser Ala Thr Gly
145 150 155 160
Pro Lys Ile Gly Glu His Tyr Val Gly Ser Gly Ser Asn Asn Asn Gln
165 170 175
Met Ser Ser Asp His Ser Ser Ser Met Asn Asp Leu Tyr Ser Glu Gly
180 185 190
Arg Gly Glu Asp Leu Glu Ser Tyr Asp Tyr Asn Gln Met Ala Leu His
195 200 205
His Ser Thr Ser Ser Pro Asn Met Asn Ile His Val Gly Ser Pro Thr
210 215 220
Val Asn Thr Asn Thr Ser Ser Ala Ala Thr Asp Gly Asn Trp Met Arg
225 230 235 240
Tyr Leu Ser Glu Asp Ala Phe Ser Phe Thr Asn Pro Ser Phe Ser Asn
245 250 255
Cys Gly Asn Ile Ser Tyr Pro Pro Ser Lys Val Asp Ile Ala Leu Glu
260 265 270
Cys Ala Arg Leu Gln His Arg Leu Ser Met Pro Pro Leu Glu Val His
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Asp Phe Pro Gln Gly Gly Phe Val Asp Leu Arg Ile Pro Gln Ser Asp
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Ser Ile Arg Ser His Asn Pro Asn Asp His Glu Glu His Asp Ile Leu
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Gln Glu Ile Leu Ser Val Ala Gln Ala Ser Gln Glu Leu Ile Asn Gln
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Asp Met Thr Trp Gly Val Thr Ser Gly Gly Gly Ser Phe Tyr Ala Gln
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Gln Ala Gly Asp Asp Phe Ser Phe His Pro His Ala Val Gln Ile His
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Asp Met Met Asn Asn Gly Gly Gly Gly Gly Ser Ser Arg Leu Ile Met
370 375 380
Glu Asp Gln Gln His Thr Ile Ser Arg Ser Ile Glu Ile Gly Gly Phe
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Asp Lys Asp Phe Lys Thr Glu Arg Thr Val Glu Asn Leu Arg Trp Val
405 410 415
Gly Met Ser Ser Lys Asp Leu Glu Lys Asn Phe Ser Glu Asp Tyr Lys
420 425 430
Thr Val Pro Ile Glu Thr Ile Ser Cys Phe Gln Thr Ser Glu Asp Gln
435 440 445
Glu Val Arg Gly Glu Ile Asp His Gln Ser Asn Phe Asn Glu Phe Asn
450 455 460
Asp Arg Glu Ala Asn Asp Phe Pro Phe Gly Phe Thr Asp Asp Asn Leu
465 470 475 480
Asn Asp Asn Phe Leu Asp Asp Ser His Glu Val Asp Asp Phe Ser Ser
485 490 495
Thr Pro Asn Phe Glu Val His Glu Lys Ile Glu Val Ser His Gly Leu
500 505 510
Phe Val Ser Thr Arg Gln Val Ala Glu Thr Phe Phe His Gln Ile Val
515 520 525
Pro Ser Glu Ile Val Lys Val Tyr Leu Asn Pro Val Ile Thr Thr His
530 535 540
His Ser Pro Met Thr Lys Pro Asp Ser Thr Thr Lys Pro Lys Lys Pro
545 550 555 560
Trp Arg Lys Ile Val Ser Pro Ser Ile Thr Ile Ile Ala Leu Leu Leu
565 570 575
Thr Tyr Tyr Cys Val Tyr Phe Gly Glu His Leu Glu Asp Glu Lys Leu
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Arg Asp Asn Phe Val Asp Asn Thr Ser Lys Gly Val Lys Arg Arg Glu
595 600 605
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Gly Thr Leu Met Trp Lys Asn Tyr Lys Lys Arg Asn Trp Phe Val Gly
625 630 635 640
Ser Ile Gly Gly Ser Ile Cys Ser Met Val Leu Asn Gln Val Trp Pro
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<210> 3
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<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
atggcacctg tttcgttgcc 20
<210> 4
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
ctatggggaa ttaggtgcaa tatgc 25
<210> 5
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 5
tgaagggaaa aggggttggg 20
<210> 6
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 6
tccacaactt ctcgcagcct ct 22
<210> 7
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
gctaaggttg ccaaggctgt c 21
<210> 8
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
taaggtattg actttctttg tctga 25
Claims (7)
1.一种茶树NAC转录因子在构建过表达黄酮醇的植株中的应用，其特征在于：所述茶树NAC转录因子的氨基酸序列如SEQ ID NO：2所示;所述植株为烟草。
2.如权利要求1所述的应用，其特征在于：所述茶树NAC转录因子的编码基因如SEQ IDNO：1所示。
3.如权利要求1或2所述的应用，其特征在于：以所述茶树NAC转录因子的编码基因构建重组质粒，将所述重组质粒转入农杆菌中，得到重组基因工程菌。
4.如权利要求3所述的应用，其特征在于：所述重组质粒的载体为pCAMBIA2300载体。
5.如权利要求3所述的应用，其特征在于所述重组质粒按如下方法构建：
（1）以从Camellia sinensis CV. Zijuan新梢叶片提取的总RNA为模板，反转录得到cDNA，以所述cDNA为模板，通过以下引物进行PCR扩增得到茶树NAC转录因子的编码基因：pCAMBIA2300-CsNAC086-F：5’-ATGGCACCTGTTTCGTTGCC-3’，
pCAMBIA2300-CsNAC086-R：5’-CTATGGGGAATTAGGTGCAATATGC-3’；
（2）用限制性内切酶BamH I与Sal I对 pCAMBIA2300载体进行双酶切，得到线性化载体；以ClonExpress ® II One Step Cloning Kit对步骤（1）所述茶树NAC转录因子的编码基因和所述线性化载体进行一步克隆，以所得一步克隆的产物转化感受态细胞，经含卡那霉素的培养基筛选和测序验证后，挑选阳性克隆培养并提取质粒，即得到所述重组质粒。
6.如权利要求3所述的应用，其特征在于所述重组基因工程菌按如下方法构建：热激法将所述重组质粒转入农杆菌GV3101感受态细胞，经含所述重组质粒的载体所携带的抗性基因指向的抗生素的YEB固体培养基筛选，菌落PCR检验，得到所述重组基因工程菌。
7.如权利要求3所述的应用，其特征在于所述过表达黄酮醇的植株按如下方法构建：
将本氏烟草Nicotiana benthamiana无菌苗上部完全展开的幼嫩叶片裁剪成小块浸泡于所述重组基因工程菌的菌液中10 min，所述重组基因工程菌的菌液的OD600为0.6；取出叶片后，转移无菌滤纸上吸干液体，然后于共培养培养基上28℃暗培养三天；然后转入出芽选择培养基中在28℃的培养温度下按照光照16 hr/d，黑暗8 hr/d进行培养，进行抗性芽的筛选；切下所得抗性芽转入壮苗选择培养基，在28℃的培养温度下按照光照16 hr/d，黑暗8hr/d进行生根培养，即得到所述过表达黄酮醇的植株。
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| A membrane-bound NAC transcription factor as an integrator of biotic and abiotic stress signals;Pil Joon Seo et al.;《Plant Signal Behav》;第481-483页 * |
| 拟南芥次生生长相关NAC转录因子保守结构域分析;陈莹等;《西北农林科技大学学报（自然科学版）》;第185-194页 * |
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