CN115192650A - Natural galenical preparation for relieving animal stress and preparation method thereof - Google Patents
Natural galenical preparation for relieving animal stress and preparation method thereof Download PDFInfo
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- A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
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- A61K36/76—Salicaceae (Willow family), e.g. poplar
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention discloses a natural galenical preparation for relieving animal stress and a preparation method thereof, wherein the natural galenical preparation comprises the following raw materials in parts by weight: 0.1-20 parts of citrus; 0.1-10 parts of green olive; 0.5-10 parts of pine cone; 0.05-5 parts of fire mulberry leaves; 0.05-5 parts of buxine; 0.01-3 parts of plant extract; the plant extract is prepared by mixing the Amaranthus caudatus extract and the Salicornia europaea extract according to the weight ratio of 2-4, and the plant extract and the Salicornia europaea extract are matched for use, so that the effects of relieving and reducing blood pressure and tranquilizing the mind are achieved, the secretion and release of adrenocorticotropic hormone in the hypothalamus caused by the stress response of an animal can be obviously reduced, the over-activation of the hypothalamus-pituitary-adrenal axis is avoided, and the stress response and the brought damage to the body function of the animal are relieved. The preparation completely adopts natural plant components, does not contain any chemical additive, has no toxic or side effect under normal administration, has wide raw material source and low cost, can play an effective relieving role in aiming at various types of animal stress reactions, and is suitable for popularization and application.
Description
Technical Field
The invention relates to the technical field of veterinary drugs, and particularly relates to a natural galenical preparation for relieving animal stress and a preparation method thereof.
Background
The stress response of animals is an adaptive response of animal organisms to the stimulation of internal and external environment changes. The stress response is generated by stimulation of various factors, including climate factors, environmental scaring factors, feeding management factors, nutritional factors, group feeding stress, density stress, transportation stress, weaning stress, pathogenic factors or diseases, genetic factors, propagation factors and the like, and the stress factors, namely the stressors, stimulate animals to cause harm to animal bodies, and are expressed in that the animals are in mental retardation, loss of appetite, extreme excitement, mania and reduced ingestion; the anabolism is weakened, the feed conversion rate is reduced, the production performance is reduced, the immunity is weakened, and the clinical symptoms such as allergy and the like are caused.
In order to relieve the stress response of animals, various types of pharmaceutical preparations appear on the market at present, but the pharmaceutical preparations basically contain chemical components or take components required by organisms as direct raw materials, so that the price is high, and more or less toxic and side effects exist, and other physiological functions of the animals are influenced.
Disclosure of Invention
The invention aims to provide a natural galenical preparation for relieving animal stress and a preparation method thereof, which solve the defects of high price and toxic and side effects of the traditional medicinal preparation for relieving animal stress.
The invention realizes the purpose through the following technical scheme:
a natural galenical preparation for relieving animal stress comprises the following raw materials in parts by weight:
0.1-20 parts of citrus;
0.1-10 parts of green olive;
0.5-10 parts of pine cone;
0.05-5 parts of fire mulberry leaves;
0.05-5 parts of buxine;
0.01-3 parts of plant extract;
wherein the plant extract is prepared by mixing the Amaranthus caudatus extract and the Salicornia europaea extract according to the weight ratio of 2.
The further improvement is that the plant extract is formed by mixing the Amaranthus caudatus extract and the Salicornia Bigelovii extract according to the weight ratio of 1.
The further improvement is that the preparation raw materials also comprise an auxiliary agent, wherein the auxiliary agent accounts for 1-3% of the total weight of the preparation, and the auxiliary agent is linoleic acid, oleic acid or a mixture thereof.
The invention also provides a preparation method of the natural galenical preparation for relieving animal stress, which comprises the following steps:
step one, preparing a Amaranthus caudatus extract and a Suaeda salsa extract respectively and mixing to obtain plant extracts for later use;
step two, respectively removing impurities from peeled oranges, green olive fruits, mature pinecones, leaves of fire mulberry leaves and chrysine crystal blocks, cleaning with clear water, airing, mixing, and crushing to obtain main material pulp for later use;
and step three, dispersing the main material pulp into the plant extract while stirring, and after uniformly mixing, sterilizing and packaging to obtain the natural plant preparation.
The further improvement is that the preparation method of the Amaranthus caudatus extract comprises the following steps: crushing Amaranthus caudatus seeds, adding 5-10 times of water by weight, carrying out ultrasonic treatment for 5-20min, filtering to obtain filter residue, adding 5-20 times of 75-90% ethanol solution by weight into the filter residue, carrying out reflux extraction for 1-3h, filtering to obtain filtrate and filter residue, extracting the filter residue for 1 time in the same manner, obtaining filtrate again, combining the two filtrates, concentrating, drying and crushing to obtain the Amaranthus caudatus extract.
The further improvement is that the power of the ultrasonic treatment is 150-300W, and the frequency is 30-50kHz.
The further improvement is that the preparation method of the suaeda salsa extract comprises the following steps: pulverizing branches and stems of Salicornia Bigelivii Torr, adding 1-4% lactic acid water solution 3-5 times of Salicornia Bigelivii Torr, adjusting pH to 4.5-6, heating to 45-50 deg.C, adding cellulase 0.5-3% and pectinase 0.5-2% of Salicornia Bigelivii Torr, stirring, performing enzymolysis for 40-60min, applying electric field for 5-10min during enzymolysis to obtain enzymolysis solution, filtering to obtain filtrate, freeze drying, and pulverizing to obtain the final product.
The further improvement is that the electric field intensity of the electric field treatment is 30-40kV/cm, the pulse time is 400-600 mus, and the pulse frequency is 150-250Hz.
The invention has the beneficial effects that: the citrus in the preparation is rich in vitamin C and terpenoid, and has effects of calming central nerve; the green olive is rich in nutrient element calcium, and can avoid metabolism imbalance and dysfunction of endoplasmic reticulum; the pine cone is rich in N-acetyl-5-methoxytryptamine, and is helpful for regulating mental state and improving sleep quality; the gamma-aminobutyric acid in the fire mulberry leaves is used as an inhibitory neurotransmitter and can play the roles of calming, hypnotizing and resisting convulsion; the populus euphratica alkali is beneficial to maintaining the balance of electrolyte and acid-base of an animal body; the amaranthus caudatus extract and the salicornia europaea extract are matched for use, so that the effects of relieving and reducing blood pressure and calming are achieved, the secretion and release of adrenocorticotropic hormone in hypothalamus caused by the response of an animal to a stress can be obviously reduced, the excessive activation of the hypothalamus-pituitary-adrenal axis is avoided, and the stress response and the brought damage to the body function of the animal are relieved.
The preparation completely adopts natural plant components, does not contain any chemical additive, has no toxic or side effect under normal administration, has wide raw material source and low cost, can play an effective relieving role in aiming at various types of animal stress reactions, and is suitable for popularization and application.
Drawings
FIG. 1 is a graph showing the results of measurement of corticotropin levels.
Detailed Description
The present application is described in further detail below with reference to examples, and it should be noted that the following detailed description is provided for further explanation of the present application and should not be construed as limiting the scope of the present application, and that certain insubstantial modifications and adaptations of the present application may be made by those skilled in the art based on the above-mentioned disclosure.
Example 1
A natural galenical preparation for relieving animal stress comprises the following raw materials in parts by weight: 0.1 part of citrus; 0.1 part of green olive; 10 parts of pine nuts; 5 parts of fire mulberry leaves; 5 parts of populine; 0.01 part of plant extract; wherein the plant extract is prepared by mixing the Amaranthus caudatus extract and the Suaeda salsa extract according to the weight ratio of 2.
The preparation method of the natural galenical preparation for relieving the stress of the animals comprises the following steps:
step one, preparing a Amaranthus caudatus extract and a Suaeda salsa extract respectively and mixing to obtain plant extracts for later use;
the preparation method of the Amaranthus caudatus extract comprises the following steps: crushing Amaranthus caudatus seeds, adding 5 times of water by weight, carrying out ultrasonic treatment (power is 150W and frequency is 30 kHz) for 20min, filtering to obtain filter residues, adding 5 times of 75% ethanol solution by weight into the filter residues, carrying out reflux extraction for 1h, filtering to obtain filtrate and filter residues, extracting the filter residues again for 1 time in the same way, obtaining filtrate again, combining the two filtrates, concentrating, drying and crushing to obtain the Amaranthus caudatus extract;
the preparation method of the suaeda salsa extract comprises the following steps: crushing branches and stems of suaeda salsa, adding 1% lactic acid water solution with the weight 3 times that of the suaeda salsa, adjusting the pH value to 4.5, heating to 45 ℃, adding cellulase with the weight 0.5% of the weight of the suaeda salsa and pectinase with the weight 0.5%, uniformly stirring, performing enzymolysis for 60min, applying an electric field for 5min during the enzymolysis period for treatment (the electric field intensity is 40kV/cm, the pulse time is 600 mus, and the pulse frequency is 250 Hz), obtaining an enzymolysis solution, filtering to obtain a filtrate, and performing freeze drying and crushing on the filtrate to obtain the suaeda salsa extract;
step two, respectively removing impurities from peeled oranges, green olive fruits, mature pine nuts, leaves of fire mulberry leaves and chrysin crystal blocks, cleaning the crystal blocks with clear water, drying the crystal blocks in the air, mixing the crystal blocks, and crushing the crystal blocks to obtain main material pulp for later use;
and step three, dispersing the main material pulp into the plant extract while stirring, and after uniformly mixing, sterilizing and packaging to obtain the natural plant preparation.
Example 2
A natural galenical preparation for relieving animal stress comprises the following raw materials in parts by weight: 10 parts of citrus; 5 parts of green olive; 5 parts of pine cone; 3 parts of fire mulberry leaves; 3 parts of buxine; 1 part of plant extract; wherein the plant extract is prepared by mixing the Amaranthus caudatus extract and the Suaeda salsa extract according to the weight ratio of 1.
The preparation method of the natural galenical preparation for relieving the stress of the animals comprises the following steps:
step one, preparing a Amaranthus caudatus extract and a Suaeda salsa extract respectively and mixing to obtain plant extracts for later use;
the preparation method of the Amaranthus caudatus extract comprises the following steps: crushing Amaranthus caudatus seeds, adding 6 times of water by weight, carrying out ultrasonic treatment (power is 200W and frequency is 40 kHz) for 13min, filtering to obtain filter residue, adding 12 times of 80% ethanol solution by weight into the filter residue, carrying out reflux extraction for 2h, filtering to obtain filtrate and filter residue, extracting the filter residue for 1 time in the same way, obtaining filtrate again, combining the two filtrates, concentrating, drying and crushing to obtain the Amaranthus caudatus extract;
the preparation method of the suaeda salsa extract comprises the following steps: crushing branches and stems of the suaeda salsa, adding 2% of lactic acid aqueous solution 4 times the weight of the suaeda salsa, adjusting the pH value to 5, heating to 50 ℃, adding cellulase 2% of the weight of the suaeda salsa and pectinase 1%, uniformly stirring, performing enzymolysis for 50min, applying an electric field for 6min during the enzymolysis period, obtaining an enzymolysis solution, filtering to obtain a filtrate, and performing freeze drying and crushing on the filtrate to obtain the suaeda salsa extract;
step two, respectively removing impurities from peeled oranges, green olive fruits, mature pine nuts, leaves of fire mulberry leaves and chrysin crystal blocks, cleaning the crystal blocks with clear water, drying the crystal blocks in the air, mixing the crystal blocks, and crushing the crystal blocks to obtain main material pulp for later use;
and step three, dispersing the main material pulp into the plant extract while stirring, and after uniformly mixing, sterilizing and packaging to obtain the natural plant preparation.
Example 3
A natural galenical preparation for relieving animal stress comprises the following raw materials in parts by weight: 20 parts of citrus; 10 parts of green olive; 0.5 part of pine cone; 0.05 part of fire mulberry leaf; 0.05 part of buxine; 3 parts of plant extract and 1 part of linoleic acid; wherein the plant extract is prepared by mixing the Amaranthus caudatus extract and the Salicornia europaea extract according to the weight ratio of 1.
The preparation method of the natural galenical preparation for relieving the stress of the animals comprises the following steps:
step one, preparing a Amaranthus caudatus extract and a Suaeda salsa extract respectively and mixing to obtain plant extracts for later use;
the preparation method of the Amaranthus caudatus extract comprises the following steps: crushing Amaranthus caudatus seeds, adding 10 times of water by weight, carrying out ultrasonic treatment (power is 300W and frequency is 50 kHz) for 5min, filtering to obtain filter residues, adding 20 times of 90% ethanol solution by weight into the filter residues, carrying out reflux extraction for 3h, filtering to obtain filtrate and filter residues, extracting the filter residues again for 1 time in the same way, obtaining filtrate again, combining the two filtrates, concentrating, drying and crushing to obtain the Amaranthus caudatus extract;
the preparation method of the suaeda salsa extract comprises the following steps: crushing branches and stems of suaeda salsa, adding 4% lactic acid water solution which is 5 times of the weight of the suaeda salsa, adjusting the pH value to 6, heating to 50 ℃, adding cellulase and pectinase which are 3% of the weight of the suaeda salsa, uniformly stirring, performing enzymolysis for 40min, applying an electric field for 10min during the enzymolysis (the electric field strength is 30kV/cm, the pulse time is 400 mus, and the pulse frequency is 150 Hz), obtaining an enzymolysis solution, filtering to obtain a filtrate, and performing freeze drying and crushing on the filtrate to obtain the suaeda salsa extract;
step two, respectively removing impurities from peeled oranges, green olive fruits, mature pine nuts, leaves of fire mulberry leaves and chrysin crystal blocks, cleaning the crystal blocks with clear water, drying the crystal blocks in the air, mixing the crystal blocks, and crushing the crystal blocks to obtain main material pulp for later use;
and step three, dispersing the main material pulp into the plant extract while stirring, and after uniformly mixing, sterilizing and packaging to obtain the natural plant preparation.
Comparative example 1
A natural galenical preparation for relieving animal stress comprises the following raw materials in parts by weight: 200 parts of citrus; 10 parts of green olive; 0.5 part of pine cone; 0.05 part of fire mulberry leaf; 0.05 part of buxine; 3 parts of plant extract and 1 part of linoleic acid; wherein the plant extracts are all Amaranthus caudatus extracts.
The preparation method of the natural galenical preparation for relieving the stress of the animals comprises the following steps:
step one, preparing a Amaranthus caudatus extract as a plant extract for later use;
the preparation method of the Amaranthus caudatus extract comprises the following steps: crushing Amaranthus caudatus seeds, adding 10 times of water by weight, carrying out ultrasonic treatment (power is 300W and frequency is 50 kHz) for 5min, filtering to obtain filter residues, adding 20 times of 90% ethanol solution by weight into the filter residues, carrying out reflux extraction for 3h, filtering to obtain filtrate and filter residues, extracting the filter residues again for 1 time in the same way, obtaining filtrate again, combining the two filtrates, concentrating, drying and crushing to obtain the Amaranthus caudatus extract;
step two, respectively removing impurities from peeled oranges, green olive fruits, mature pine nuts, leaves of fire mulberry leaves and chrysin crystal blocks, cleaning the crystal blocks with clear water, drying the crystal blocks in the air, mixing the crystal blocks, and crushing the crystal blocks to obtain main material pulp for later use;
and step three, dispersing the main material pulp into the plant extract while stirring, and after uniformly mixing, sterilizing and packaging to obtain the natural plant preparation.
Comparative example 2
A natural galenical preparation for relieving animal stress comprises the following raw materials in parts by weight: 20 parts of citrus; 10 parts of green olive; 0.5 part of pine cone; 0.05 part of fire mulberry leaf; 0.05 part of buxine; 3 parts of plant extract and 1 part of linoleic acid; wherein the plant extracts are all the suaeda salsa extracts.
The preparation method of the natural galenical preparation for relieving the stress of the animals comprises the following steps:
step one, preparing a suaeda salsa extract as a plant extract for later use;
the preparation method of the suaeda salsa extract comprises the following steps: crushing branches and stems of the suaeda salsa, adding 4% of lactic acid aqueous solution which is 5 times of the weight of the suaeda salsa, adjusting the pH value to 6, heating to 50 ℃, adding cellulase and pectinase which are 3% of the weight of the suaeda salsa, uniformly stirring, performing enzymolysis for 40min, applying an electric field for 10min during the enzymolysis (the electric field intensity is 30kV/cm, the pulse time is 400 mus, and the pulse frequency is 150 Hz), obtaining an enzymolysis solution, filtering to obtain a filtrate, and performing freeze drying and crushing on the filtrate to obtain the suaeda salsa extract;
step two, respectively removing impurities from peeled oranges, green olive fruits, mature pine nuts, leaves of fire mulberry leaves and chrysin crystal blocks, cleaning the crystal blocks with clear water, drying the crystal blocks in the air, mixing the crystal blocks, and crushing the crystal blocks to obtain main material pulp for later use;
and step three, dispersing the main material pulp into the plant extract while stirring, and after uniformly mixing, sterilizing and packaging to obtain the natural plant preparation.
And (4) performance testing:
1. adrenocorticotropic hormone (ACTH) level test
A plurality of mice (female and male half-bodies, the weight of which is between 18 and 22g, purchased from great laboratory animals Co., ltd.) under the same breeding condition are taken and randomly divided into two groups, wherein one group comprises 60 mice as stress mice, the other group comprises 15 mice as normal mice, and all the mice are respectively placed in mouse cages. All stressed mice were placed at room temperature (25 ℃) and shaken continuously for 8h on a shaker at 180/min, while normal mice were placed only at room temperature (25 ℃) without any treatment. The stressed mice were randomly divided into 4 groups of 15 mice each, and immediately after shaking, the mice were subjected to gavage operation by separately feeding the preparations obtained in example 3 (first group), comparative example 1 (second group), and comparative example 2 (third group) and physiological saline (fourth group), and the normal mice were fed with physiological saline (fifth group) at the same time, and the gavage amounts were all 20ml/kg.
Femoral vein bleeds were performed at 6 time points 2, 4, 8, 12, 24, 36 hours after completion of the gavage procedure, with 5 per group collected at each time point. The blood sampling mode is as follows: injecting needle to puncture femoral vein for blood drawing, taking two blood portions of about 0.2-0.3 ml per white mouse, injecting one portion into the injection chamber, and using the injection chamber as 10%One part of the tube washed with DTA dipotassium and aprotinin was filled into a tube previously washed with heparin, two parts were centrifuged at 3000r/min and 4 ℃ for 10min, and plasma was separated and stored in a refrigerator at-20 ℃ for further use. Before the measurement of the frozen sample, the sample is put in room temperature Or cold water for re-melting, centrifuged again at 4 ℃ at 300Or/min for 5min, and then the supernatant is taken for the measurement of the adrenocorticotropic hormone level. The determination adopts ELISAL kit detection (purchased from Beijing northern biotechnological institute, and preserved at 2-8 ℃ in the dark place, and the composition of the ACTH standard substance (S) 0 ～S 5 )、 125 I-ACTH (red), rabbit anti-ACTH antiserum (blue), donkey anti-rabbit immunoseparation agent, ACTH buffer (colorless). ) The process is carried out.
Final corticotropin level measurements referring to fig. 1, it can be seen that the first group, gavaged with the natural galenical formulation prepared in example 3, had the highest ACTH concentration at 2h, but less than 20pg/mL, followed by a gradual decline in ACTH concentration, at 36h, even lower than the fifth group; the second group and the third group respectively adopt the natural plant preparations prepared in the comparative examples 1 and 2 for intragastric administration, and because only the Amaranthus caudatus extract and the Suaeda salsa extract are respectively adopted in the preparations, the ACTH concentration reaches the highest point in 4h and is obviously higher than the level of the first group, the subsequent ACTH concentration is gradually reduced, but still reaches about 23pg/mL in 36 h; the fourth group adopts normal saline for intragastric administration, the ACTH concentration of the group is the highest and exceeds 40pg/mL in 4 hours; the fifth group served as a blank control, and normal mice stabilized at ACTH concentrations around 15 pg/mL. Therefore, the natural plant preparation prepared by the invention can avoid over-activation of hypothalamus-pituitary-adrenal axis and reduce secretion and release of adrenocorticotropic hormone, thereby achieving the effect of effectively relieving stress response.
2. Breeding test
Selecting 50 healthy domestic pigs of 120 days old, numbering one by one, weighing, dividing into 5 groups, and recording 10 pigs in each group as 1-5 groups. Wherein group 1 was fed with a basal diet incorporating the natural plant preparation prepared in example 3, group 2 was fed with a basal diet incorporating the natural plant preparation prepared in comparative example 1, group 3 was fed with a basal diet incorporating the natural plant preparation prepared in comparative example 2, and groups 4 and 5 were fed with basal diets, the incorporation ratio being 0.5% by weight.
The feeding mode specifically comprises the following steps: carrying out transport stimulation on the pigs in the groups 1-4, wherein the pigs are transported and stimulated for 0.5 hour on a bumpy road by a truck in the morning every day from the day 1 of feeding; for group 5, no transport stimulation was performed.
The growth environment conditions of the 5 groups are consistent, the temperature of the piggery is kept at about 25 ℃, the relative humidity is kept at about 70%, the piggery is raised in a high bed, the piggery is fed freely, water is drunk freely, feces are cleared twice every day, the piggery is kept clean, and the mental state and the number of diarrhea heads of each group of domestic pigs are monitored and recorded at any time.
And after 15 days, weighing each pig, calculating the average daily feed intake, daily gain and feed conversion ratio of each pig, and calculating the average value of each data of each group.
The calculation result statistics obtain the following table:
as can be seen from the table above, the group 1 is fed by blending the natural plant preparation prepared in example 3, so that after transportation stimulation, the domestic pigs still keep good mental state, diarrhea is less, daily average feed intake reaches 2234.4g, daily average weight gain reaches 912.3g, feed-meat ratio is controlled at 2.45, and each item of data is basically close to the group 5, thereby indicating that the influence of transportation stimulation on the domestic pigs is small or even negligible due to the use of the natural plant preparation; and the 2 nd group and the 3 rd group have obviously poor effects because only the Amaranthus caudatus extract and the Amaranthus caudatus extract are respectively adopted in the preparation, wherein the daily average feed intake is respectively reduced by 6.2 percent and 7.7 percent, the daily average weight gain is respectively reduced by 7.4 percent and 8.8 percent, and the feed-meat ratio is also improved, thereby showing that the Amaranthus caudatus extract and the Amaranthus caudatus extract have the best effects only when being matched for use.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (8)
1. The natural galenical preparation for relieving animal stress is characterized by comprising the following raw materials in parts by weight:
0.1-20 parts of citrus;
0.1-10 parts of green olive;
0.5-10 parts of pine cone;
0.05-5 parts of fire mulberry leaves;
0.05-5 parts of buxine;
0.01-3 parts of plant extract;
wherein the plant extract is prepared by mixing the Amaranthus caudatus extract and the Salicornia europaea extract according to the weight ratio of 2.
2. The natural botanical preparation for relieving animal stress as claimed in claim 1, wherein the botanical extract is prepared by mixing Amaranthus caudatus extract and Salicornia europaea extract in a weight ratio of 1.
3. The natural galenical formulation for relieving animal stress according to claim 1, wherein the preparation raw materials further comprise an auxiliary agent, the auxiliary agent accounts for 1-3% of the total weight of the formulation, and the auxiliary agent is linoleic acid, oleic acid or a mixture thereof.
4. A method of preparing a natural botanical preparation for relieving animal stress as claimed in any of claims 1 to 3, comprising the steps of:
step one, preparing a Amaranthus caudatus extract and a Suaeda salsa extract respectively and mixing to obtain plant extracts for later use;
step two, respectively removing impurities from peeled oranges, green olive fruits, mature pine nuts, leaves of fire mulberry leaves and chrysin crystal blocks, cleaning the crystal blocks with clear water, drying the crystal blocks in the air, mixing the crystal blocks, and crushing the crystal blocks to obtain main material pulp for later use;
and step three, dispersing the main material pulp into the plant extract while stirring, and after uniformly mixing, sterilizing and packaging to obtain the natural plant preparation.
5. The method for preparing a natural galenical preparation for relieving animal stress according to claim 4, wherein the method for preparing the Amaranthus caudatus extract comprises the following steps: crushing Amaranthus caudatus seeds, adding 5-10 times of water by weight, carrying out ultrasonic treatment for 5-20min, filtering to obtain filter residue, adding 5-20 times of 75-90% ethanol solution by weight into the filter residue, carrying out reflux extraction for 1-3h, filtering to obtain filtrate and filter residue, extracting the filter residue for 1 time in the same manner, obtaining filtrate again, combining the two filtrates, concentrating, drying and crushing to obtain the Amaranthus caudatus extract.
6. The method for preparing a natural galenical preparation for relieving animal stress according to claim 5, wherein the ultrasonic treatment has a power of 150 to 300W and a frequency of 30 to 50kHz.
7. The method for preparing a natural galenical preparation for relieving animal stress according to claim 4, wherein the Salicornia herbacea extract is prepared by the following steps: pulverizing branches and stems of Salicornia Bigelivii Torr, adding 1-4% lactic acid water solution 3-5 times of the weight of Salicornia Bigelivii Torr, adjusting pH to 4.5-6, heating to 45-50 deg.C, adding cellulase 0.5-3% and pectinase 0.5-2% of the weight of Salicornia Bigelivii Torr, stirring, performing enzymolysis for 40-60min, applying electric field for 5-10min during enzymolysis to obtain enzymolysis solution, filtering to obtain filtrate, freeze drying and pulverizing to obtain the extract.
8. The method for preparing a natural galenical preparation for relieving animal stress according to claim 7, wherein the electric field treatment has an electric field strength of 30 to 40kV/cm, a pulse time of 400 to 600 μ s, and a pulse frequency of 150 to 250Hz.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379977A (en) * | 2015-11-30 | 2016-03-09 | 山东绿都安特动物药业有限公司 | Fattening cattle and sheep feed additive capable of relieving heat stress and improving meat quality |
| CN105748861A (en) * | 2014-12-16 | 2016-07-13 | 北京大北农动物保健科技有限责任公司 | Traditional Chinese medicine composition for resisting stress reaction, and preparation method and application thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105748861A (en) * | 2014-12-16 | 2016-07-13 | 北京大北农动物保健科技有限责任公司 | Traditional Chinese medicine composition for resisting stress reaction, and preparation method and application thereof |
| CN105379977A (en) * | 2015-11-30 | 2016-03-09 | 山东绿都安特动物药业有限公司 | Fattening cattle and sheep feed additive capable of relieving heat stress and improving meat quality |
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| Title |
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| 刘孝然，等: "动物运输应激预防药物和添加剂的研究进展" * |
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