TWI802101B - Compositions of red-fleshed apples and cherry plums and their use in lowering blood sugar - Google Patents

Abstract

The invention relates to the fields of food and medicine, in particular to a composition of red-fleshed apples and cherry plums with the effect of lowering blood sugar. The weight ratio of the red-flesh apples and cherry plums is 1:10 to 10:1. Zebrafish and mouse animal experiments show that the composition of red-fleshed apples and cherry plums has a synergistic effect on hypoglycemic efficacy, and the hypoglycemic effect of the composition is better than that of red-fleshed apples or cherry plums alone. The product forms of the red flesh apple and cherry plum composition include fruit juice, jam, freeze-dried powder, oral liquid products, capsules, tablets and the like.

Description

Compositions of red-fleshed apples and cherry plums and their use in lowering blood sugar

The invention relates to the fields of food and medicine, in particular to a composition of red-fleshed apples and cherry plums and their application in reducing blood sugar.

Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia caused by absolute or relative insulin secretion deficiency and (or) insulin utilization disorder. Statistics from the International Diabetes Federation (IDF) in 2017 show that the prevalence of diabetes in Chinese adults ranges from 9.7% to 11.6%, and the number of people with diabetes is about 110 million, ranking first in the world.

At present, there is no cure for diabetes. The clinically used drugs for the treatment of diabetes mainly include sulfonylureas (such as glipizide, glimepiride, gliclazide, glibenclamide, etc.), biguanides ( Metformin), α-glucosidase inhibitors (acarbose, voglibose), insulin sensitizers (rosiglitazone, pioglitazone, etc.), insulin secretagogues (benzoic acid derivatives), DPP-4 Inhibitors (saxagliptin, sitagliptin, etc.), SGLT-2 inhibitors (dapagliflozin, empagliflozin, etc.), and insulin therapy. However, hypoglycemia is a common serious side effect of hypoglycemic therapy. α-Glucosidase inhibitors, biguanides and insulin-stimulating agents generally do not cause hypoglycemia when used alone, but it may still occur when used in combination with other drugs. Biguanide hypoglycemic drugs are more harmful to the stomach. Therefore, it is necessary to find natural resources with good effect and no side effects for the prevention and treatment of diabetes.

Red flesh apple ( Malus neidzwetzkyana Dieck) is a Rosaceae (Rosaceae) Malus Mill plant. The flesh of its fruit is red, and the color of the fruit is mainly caused by the accumulation of anthocyanins. At present, there are many varieties of red-fleshed apples around the world, such as Hongxun series in Xinjiang, China, Robert Crab in the United States, Ruby Sweet in Japan, and Redlove in Switzerland. The flowers, branch and leaf colors, fruit size, coloring degree, taste and flavor of each variety of red flesh apple are different. Red-fleshed apples in Xinjiang play an important role in the origin of red-fleshed apples.

Cherry plum ( Prunus cerasifera Ehrhart), commonly known as wild sour plum, belongs to the genus Prunus in the family Rosaceae. It is an extremely precious, rare and endangered original wild forest fruit in the world. According to the records of "Chinese Tree Records" and the research of the National Institute of Wild Plants, wild cherry plums are only found in the western part of the Tianshan Mountains in Xinjiang, the Caucasus, and the mountains of Asia Minor, and wild resources are extremely scarce. Cherry and plum fruits have a long history of eating in the Xinjiang region of my country. The Kazak and Mongolian herdsmen who live in Daxigou, Tianshan Mountains mainly eat beef and mutton, but few of them suffer from hyperlipidemia and high blood pressure. This is different from the herdsmen It is related to the habit of picking wild cherry plums for a long time to make jam or drying the fruits to make tea. Cherry plums include many varieties, and according to the color, they mainly include black fruit, red fruit, purple fruit, purple black fruit, yellow fruit and so on.

Red-fleshed apples and cherry plums are rich in various amino acids, vitamins, minerals, and functional components such as anthocyanins, polyphenols, and flavonoids, and have high development value. However, the current research is still relatively limited, mainly focusing on chemical components and antioxidant aspects, and is still in the preliminary research stage.

After a lot of experimental research, the inventors of the present application found that the combination of red-fleshed apples (or red-fleshed apple extracts) and cherry plums (or cherry plum extracts) has a synergistic effect on lowering blood sugar.

Therefore, in the first aspect of the present invention, the present invention provides the use of the composition in the preparation of medicine (such as traditional Chinese medicine, Chinese patent medicine, etc.), common food or health food, the medicine (such as Chinese medicine, Chinese patent medicine, etc.), common The food or health food is used for lowering blood sugar or for preventing and/or treating blood sugar diseases, wherein the composition comprises: a first component that is a red-fleshed apple or a red-fleshed apple extract; and The second component, the second component is cherry plum or cherry plum extract.

In some embodiments, the glycemic disease is selected from diabetes and diabetes-related diseases (such as type I diabetes, type II diabetes, diabetic nephropathy, etc.).

In some embodiments, the weight ratio of the first component to the second component is 1:10 to 10:1 (such as 1:5-10:1, 1:2-10:1, 1:1- 10:1, 1:10-5:1, 1:5-5:1, 1:2-5:1, 1:1-5:1, 1:10-2:1, 1:5-2: 1, 1:2-2:1, 1:1-2:1, 1:10-1:1, 1:5-1:1, 1:2-1:1, 1:10-5:1, 1:10-2:1, 1:10-1:1, 1:5-10:1, 1:5-5:1, 1:5-2:1, 1:5-1:1, 1:1: 2-10:1, 1:2-5:1, 1:2-2:1, 1:2-1:1, 1:1-10:1, 1:1-5:1, 1:1- 2:1, such as 1:5, 1:2, 1:1, 2:1, 5:1).

In some embodiments, the red apple extract is obtained by one of the following extraction methods: leaching extraction, enzymatic ultrasonic extraction, ultra-high pressure extraction, subcritical extraction or other extraction methods.

In some embodiments, the cherry plum extract is obtained by one of the following extraction methods: leaching extraction, enzymatic ultrasonic extraction, ultra-high pressure extraction, subcritical extraction or other extraction methods.

In some embodiments, the composition is in the form of fresh fruit, fruit juice, jam, fruit powder (such as freeze-dried powder), candy (such as compressed candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or pastry, etc. form exists. In other words, the product form of the composition can be fresh fruit, fruit juice, jam, fruit powder (such as freeze-dried powder), candy (such as compressed candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or pastry wait. That is to say, the composition can be made into fresh fruit, fruit juice, jam, fruit powder (such as freeze-dried powder), candy (such as compressed candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or pastry, etc.

In some embodiments, the red-fleshed apple refers to the fruit of a plant of the genus Malus in the family Rosaceae with red flesh.

In some embodiments, the red meat apple is selected from: Hongxun No. 1 (Hongxun No. 1), Hongxun No. 3 (Hongxun No. 3), Hongxun No. 6 (Hongxun No. 6), Xia Hongrou, Dai Hong, Red Love, Roberts Crab, Red Dream, Ruby Sweet, etc.

In some embodiments, the cherry plum is selected from the group consisting of red fruit of plum cherry, black fruit of plum cherry, purple fruit of plum cherry, purple black fruit of plum cherry, yellow fruit of plum cherry, and yellow fruit of plum cherry.

In a second aspect of the present invention, the present invention provides a combination product comprising: A first product, the first product being the aforementioned composition; The second product, the second product is a drug for preventing and/or treating blood sugar disorders.

In some embodiments, the glycemic disease is selected from diabetes and diabetes-related diseases (such as type I diabetes, type II diabetes, diabetic nephropathy, etc.).

In some embodiments, the drug for preventing and/or treating blood sugar disorders is selected from sulfonylurea drugs (such as glipizide, glimepiride, gliclazide, glibenclamide, etc.), biguanide drugs (Metformin), α-glucosidase inhibitors (acarbose, voglibose), insulin sensitizers (rosiglitazone, pioglitazone, etc.), insulin secretagogues (benzoic acid derivatives), DPP- 4 Inhibitors (saxagliptin, sitagliptin, etc.), SGLT-2 inhibitors (dapagliflozin, empagliflozin, etc.), insulin, etc.

In some embodiments, the weight ratio of the first product to the second product (such as metformin or dapagliflozin) is 20000:0.75 to 20000:600 (such as 20000:0.75, 20000:1.5, 20000:2, 20000 : 2.5, 20000: 3, 20000: 3.5, 20000: 4, 20000: 4.5, 20000: 5, 20000: 5.5, 20000: 6, 20000: 75, 20000: 150, 20000: 200, 20000: 250, 20000: 300 . .

In some embodiments, when the second product is metformin, the weight ratio of the first product to the second product is 20000:75 to 20000:600 (such as 20000:75, 20000:150, 20000:200, 20000: 250, 20000: 300, 20000: 350, 20000: 400, 20000: 450, 20000: 500, 20000: 550, 20000: 600; 20000: 250, 20000: 300).

In some embodiments, when the second product is dapagliflozin, the weight ratio of the first product to the second product is 20000:0.75 to 20000:6 (such as 20000:0.75, 20000:1.5, 20000:2 , 20000: 2.5, 20000: 3, 20000: 3.5, 20000: 4, 20000: 4.5, 20000: 5, 20000: 5.5, 20000: 6; : 2, 20000: 2.5, 20000: 3).

In the third aspect of the present invention, the present invention provides the use of the aforementioned combination product in the preparation of medicine (such as traditional Chinese medicine, Chinese patent medicine, etc.), common food or health food. Or health food is used for lowering blood sugar, or used for preventing and/or treating blood sugar disease.

In the fourth aspect of the present invention, the present invention provides the aforementioned composition or the aforementioned combination product, which is used for lowering blood sugar, or used for preventing and/or treating blood sugar disorders.

In the fifth aspect of the present invention, the present invention provides a method for lowering blood sugar, or preventing and/or treating blood sugar disorders, which comprises administering the aforementioned composition or the aforementioned combination product to individuals in need thereof.

In some embodiments, the above-mentioned blood sugar disease is selected from diabetes and diabetes-related diseases (such as type I diabetes, type II diabetes, diabetic nephropathy, etc.).

In addition, the present invention also discloses a hypoglycemic combination product of red-fleshed apples and cherry plums and a preparation method thereof, including fresh fruit, fruit juice, jam, freeze-dried powder, compressed candy, oral liquid, capsules and other product forms. Animal experiments have shown that this series of products has a good synergistic hypoglycemic effect. In some embodiments, the composition of the present invention can also be used to prepare traditional Chinese medicines, Chinese patent medicines and other medicines.

In addition, in one aspect, the present application also provides a combination product, which includes a first product and a second product packaged independently, the first product is a combination product of red apples and cherry plums, and the second product is a hypoglycemic drug (such as sulfonylureas (such as glipizide, glimepiride, gliclazide, glibenclamide, etc.), biguanides (metformin), α-glucosidase inhibitors (acarbose, voltaic acid, etc.) Glibose), insulin sensitizers (rosiglitazone, pioglitazone, etc.), insulin secretagogues (benzoic acid derivatives), DPP-4 inhibitors (saxagliptin, sitagliptin, etc.), SGLT -2 inhibitors (dapagliflozin, empagliflozin, etc.), insulin, etc.).

In the present invention, "individual" includes human or non-human animal. Exemplary human subjects include human subjects suffering from a disease (eg, a disease described herein) (referred to as a patient) or normal subjects. "Non-human animals" in the present invention include all vertebrates, such as non-mammals (such as fish, birds, amphibians, reptiles) and mammals, such as non-human primates, livestock and/or domesticated animals (such as mice, sheep, dogs, cats, cows, pigs, etc.).

In the present invention, "cherry plum extract" or "red-flesh apple extract" refers to the extraction and enrichment of cherry plum or red-flesh apples through physical and/or chemical extraction and separation processes using cherry plums or red-flesh apples as raw materials It is a product formed by one or more active ingredients, which can be used as the raw material or auxiliary material of food (such as general food or health food) or medicine. Exemplary extraction methods include, but are not limited to: leaching extraction, enzymatic ultrasonic extraction, ultra-high pressure extraction and subcritical extraction.

Exemplary methods of preparing red-fleshed apple and cherry plum extracts are provided below. Extraction

In certain embodiments, the red-fleshed apple and cherry plum extracts of the present invention are obtained by leaching extraction (eg, water leaching or alcohol leaching extraction).

In certain embodiments, the leaching extraction comprises the steps of: Step 1: Deseeding or pitting red apples and cherry plums, respectively, to obtain pulp directly, or to separate juice and pomace; in some embodiments, using gauze (such as 100 to 300 mesh gauze, such 200 mesh gauze) to filter and separate the juice and pomace; Step 2: Mix the fruit pulp or pomace with the extraction medium (such as water or ethanol), and extract at normal temperature or heating (for example, 10-100°C, such as 50°C) (for example, extract for 30-100min, such as 60min), Filtration to obtain a pulp or pomace extract; in certain embodiments, the pulp or pomace is mixed with the extraction medium in a weight ratio of 1:(2-10) (eg, 1:5); in some embodiments , the ethanol is 50% to 95% ethanol; Step 3: Concentrating the fruit pulp extract or the combined liquid of the fruit juice and the pomace extract to obtain extracts of red-fleshed apples and cherry plums; in some embodiments, concentrating by rotary evaporation; In some embodiments, the temperature of the rotary evaporation is 30-60°C, such as 50°C; Step 4: Mix the extracted red flesh apple and cherry plum extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10: 1) Prepare a composition, or in step 1, mix the raw materials of red flesh apple and cherry plum according to the above-mentioned different ratios, and then extract.

In certain embodiments, the red-fleshed apple and cherry plum extracts of the present invention are obtained by water leaching extraction, and in some embodiments, the water leaching extraction comprises the following steps: Step 1: Deseeding or pitting the red-fleshed apples and cherry plums respectively, and beating to obtain pulp directly, or filtering the juices with 100 to 300 mesh gauze to obtain red-flesh apples and cherry plum juices, and separating the juice and pomace; In some embodiments, the mesh number of the gauze is selected as 200 mesh; Step 2: Red meat apple and cherry plum pulp or pomace is mixed with water according to the ratio of material to liquid: (2-10), leached and extracted at 10-100°C for 30-100min, and filtered to obtain pulp or pomace extract ; In some embodiments, the solid-liquid ratio is selected as 1:5; in some embodiments, the leaching extraction temperature is selected at 50°C; in some embodiments, the extraction time is selected for 60 minutes; Step 3: red flesh apple and cherry plum pulp extract or the combined solution of juice and pomace extract, concentrated by rotary evaporation at 30-60 ° C to obtain red flesh apple and cherry plum water extract; in some In an embodiment, the rotary evaporation temperature is selected at 50°C; Step 4: Mix the extracted red flesh apple and cherry plum water extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) to prepare a composition, or in step 1, mix the raw materials of red flesh apple and cherry plum according to the aforementioned different ratios, and then extract.

In certain embodiments, the red-fleshed apple and cherry plum extracts of the present invention are extracted by alcoholic extraction. In certain embodiments, the alcoholic extraction comprises the following steps: Step 1: Deseeding or pitting the red-fleshed apples and cherry plums respectively, and beating to obtain pulp directly, or filtering the juices with 100 to 300 mesh gauze to obtain red-flesh apples and cherry plum juices, and separating the juice and pomace; In some embodiments, the mesh number of the gauze is selected as 200 mesh; Step 2: Red meat apple and cherry plum pulp or pomace is mixed with 50% to 95% ethanol according to the ratio of material to liquid: (2-10), extracted at 10-100°C for 30-100min, and filtered to obtain fruit pulp or pomace extract; in some embodiments, the ratio of solid to liquid is selected as 1:5; in some embodiments, the concentration of ethanol used is 50%; in some embodiments, leaching extraction The temperature is selected as 50°C; in some embodiments, the extraction time is selected as 60min; Step 3: Red-flesh apple and cherry plum pulp extract or the combined liquid of juice and pomace extract is concentrated by rotary evaporation at 30-60°C, and the fruit pulp extract is directly concentrated to obtain red-flesh apple and cherry plum alcohol dip Extract the extract; in some embodiments, the rotary evaporation temperature is selected at 50° C.; optionally, the extract is evaporated to remove residual ethanol; Step 4: Mix the extracted red-flesh apple and cherry plum alcohol extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) to prepare a composition, or in step 1, mix the raw materials of red flesh apple and cherry plum according to the aforementioned different ratios, and then extract. Enzymatic Ultrasonic Extraction

In certain embodiments, the red-fleshed apple and cherry plum extracts of the present invention are obtained by enzymatic ultrasonic extraction (eg, enzymatic ultrasonic water extraction or enzymatic ultrasonic alcohol extraction).

In certain embodiments, the enzymatic ultrasonic extraction comprises the steps of: Step 1: Deseeding or pitting red apples and cherry plums, respectively, to obtain pulp directly, or to separate juice and pomace; in some embodiments, using gauze (such as 100 to 300 mesh gauze, such 200 mesh gauze) to filter and separate the juice and pomace; Step 2: Mix fruit pulp or pomace with extraction medium (such as water or ethanol) and cellulase, and extract under heating conditions (such as 30-50°C, such as 45°C) (such as extraction for 30-100min, such as 60min) , and then under ultrasonic conditions (such as 100-500W ultrasonic power), ultrasonic (for example, ultrasonic 20-60min, such as ultrasonic 30min), filtered to obtain fruit pulp or pomace extract; in some embodiments, The amount of cellulase added (based on the weight of pomace or pulp) is 0.2% to 1% (eg 0.5%); in some embodiments, the ratio of pulp or pomace to extraction medium is 1:(2- 10) mixing in a weight ratio (eg 1:5); in certain embodiments, the ethanol is 50% to 95% ethanol; Step 3: concentrating red-flesh apple and cherry plum pulp extracts or the combined liquid of juice and pomace extracts to obtain enzymatic ultrasonic extracts of red-flesh apples and cherry plums; in some embodiments, by Concentrating by rotary evaporation; in some embodiments, the temperature of the rotary evaporation is 30-60°C, such as 50°C; Step 4: Mix the extracted red flesh apple and cherry plum enzymatic ultrasonic extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5: 1. 10:1) is prepared into a composition, or in step 1, the raw materials of red flesh apple and cherry plum are mixed according to the aforementioned different ratios, and then extracted.

In some embodiments, the red-fleshed apple and cherry plum extracts of the present invention are obtained by enzymatic ultrasonic water extraction. In some embodiments, the enzymatic ultrasonic water extraction includes the following steps: Step 1: Deseeding or pitting the red-fleshed apples and cherry plums respectively, and beating to obtain pulp directly, or filtering the juices with 100 to 300 mesh gauze to obtain red-flesh apples and cherry plum juices, and separating the juice and pomace; In some embodiments, the mesh number of the gauze is selected as 200 mesh; Step 2: Mix red flesh apple and cherry plum pulp or pomace with water according to the ratio of material to liquid 1: (2-10), add 0.2% to 1% (based on the weight of pomace or pulp) of cellulose Enzyme, 30-50°C water bath extraction for 30-100min, 100-500W ultrasonic wave for 20-60min, filter to obtain fruit pulp or pomace extract; in some embodiments, the ratio of solid to liquid is selected as 1:5; in some In some embodiments, the added amount of cellulase is 0.5%; in some embodiments, the water bath temperature is 45°C; in some embodiments, the extraction time is 60min; in some embodiments, the ultrasonic power is 500W ; In certain embodiments, the ultrasound time is 30 minutes; Step 3: Red flesh apple and cherry plum pulp or the combined liquid of fruit juice and pomace extract is concentrated by rotary evaporation at 30-60°C to obtain red flesh apple and cherry plum enzymolysis ultrasonic extraction water extract; In some embodiments, the rotary evaporation temperature is selected at 50°C; Step 4: Mix the extracted red flesh apple and cherry plum enzymolysis ultrasonic water extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5 : 1, 10: 1) to prepare a composition, or in step 1, mix the raw materials of red flesh apple and cherry plum according to the aforementioned different ratios, and then extract.

In some embodiments, the red-flesh apple and cherry plum extracts of the present invention are obtained by enzymatic ultrasonic alcohol extraction. In some embodiments, the enzymatic ultrasonic alcohol extraction includes the following steps: Step 1: Deseeding or pitting the red-fleshed apples and cherry plums respectively, and beating to obtain pulp directly, or filtering the juices with 100 to 300 mesh gauze to obtain red-flesh apples and cherry plum juices, and separating the juice and pomace; In some embodiments, the mesh number of the gauze is selected as 200 mesh; Step 2: Mix red flesh apple and cherry plum pulp or pomace with 50% to 95% ethanol according to the ratio of material to liquid 1: (2-10), add 0.2% to 1% (by weight of pomace or pulp 30-50°C water bath extraction for 30-100min, 100-500W ultrasonic wave for 20-60min, and filter to obtain pulp or pomace extract; in some embodiments, the ratio of solid to liquid is selected as 1 : 5; in some embodiments, the concentration of ethanol used is 50%; in some embodiments, the cellulase addition amount is 0.5%; in some embodiments, the water bath temperature is 45 ℃; In some embodiments, the extraction time is 60min; in some embodiments, the ultrasonic power is 500W; in some embodiments, the ultrasonic time is 30min; Step 3: Red-flesh apple and cherry plum pulp extract or the combined liquid of juice and pomace extract is concentrated by rotary evaporation at 30-60°C to obtain red-flesh apple and cherry plum enzymolysis ultrasonic extraction alcohol extract; In some embodiments, the rotary evaporation temperature is selected at 50° C.; optionally, the extract is evaporated to remove residual ethanol; Step 4: Mix the extracted red flesh apple and cherry plum enzymolysis ultrasonic alcohol extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5 : 1, 10: 1) to prepare a composition, or in step 1, mix the raw materials of red flesh apple and cherry plum according to the aforementioned different ratios, and then extract. Ultra-high pressure extraction

In certain embodiments, the red-fleshed apple and cherry plum extracts of the present invention are obtained by ultra-high pressure extraction (eg, ultra-high pressure water extraction or ultra-high pressure alcohol extraction). As used herein, "ultrahigh pressure" refers to pressures in excess of 100 MPa (equivalent to 1000 atmospheres).

In certain embodiments, the ultrahigh pressure extraction comprises the steps of: Step 1: Deseeding or pitting red apples and cherry plums, respectively, to obtain pulp directly, or to separate juice and pomace; in some embodiments, using gauze (such as 100 to 300 mesh gauze, such 200 mesh gauze) to filter and separate the juice and pomace; Step 2: Mix the fruit pulp or pomace with the extraction medium (such as water or ethanol), treat it under ultra-high pressure (such as 200-600MPa, such as 400MPa) (such as 3-10min, such as 5min), and filter to obtain fruit pulp or pomace extract; in certain embodiments, the pulp or pomace is mixed with the extraction medium in a weight ratio of 1:(2-10), such as 1:5; in some embodiments, the ethanol 50% to 95% ethanol; Step 3: Concentrating red-fleshed apple and cherry plum pulp or a combined liquid juice and pomace extract to obtain an ultra-high pressure extract of red-fleshed apple and cherry plum; in certain embodiments, by rotary evaporation Concentrating; in some embodiments, the temperature of the rotary evaporation is 30-60°C, such as 50°C; Step 4: Mix the extracted red flesh apple and cherry plum UHP extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare a composition, or in step 1, mix the raw materials of red flesh apple and cherry plum according to the aforementioned different ratios, and then extract.

In certain embodiments, the red-fleshed apple and cherry plum extracts of the present invention are obtained by ultrahigh pressure water extraction, and in certain embodiments, the ultrahigh pressure water extraction comprises the following steps: Step 1: Deseeding or pitting the red-fleshed apples and cherry plums respectively, and beating to obtain pulp directly, or filtering the juices with 100 to 300 mesh gauze to obtain red-flesh apples and cherry plum juices, and separating the juice and pomace; In some embodiments, the mesh number of the gauze is selected as 200 mesh; Step 2: Red meat apple and cherry plum pulp or pomace is mixed with water according to the material-to-liquid ratio of 1: (2-10), treated with ultra-high pressure 200-600MPa for 3-10min, and filtered to obtain pulp or pomace extract; In some embodiments, the material-to-liquid ratio is selected as 1:5; in some embodiments, the ultra-high pressure is 400 MPa; in some embodiments, the ultra-high pressure treatment time is selected for 5 minutes; Step 3: Red flesh apple and cherry plum pulp extract or the combined liquid of juice and pomace extract is concentrated by rotary evaporation at 30-60°C to obtain red flesh apple and cherry plum ultra-high pressure water extract; In some embodiments, the rotary evaporation temperature is selected at 50°C; Step 4: Mix the extracted red flesh apple and cherry plum UHP water extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) to prepare a composition, or in step 1, mix the raw materials of red flesh apple and cherry plum according to the aforementioned different ratios, and then extract.

In certain embodiments, the red-fleshed apple and cherry plum extracts of the present invention are obtained by ultrahigh pressure alcohol extraction. In certain embodiments, the ultrahigh pressure alcohol extraction comprises the following steps: Step 1: Deseeding or pitting the red-fleshed apples and cherry plums respectively, and beating to obtain pulp directly, or filtering the juices with 100 to 300 mesh gauze to obtain red-flesh apples and cherry plum juices, and separating the juice and pomace; In some embodiments, the mesh number of the gauze is selected as 200 mesh; Step 2: Red meat apple and cherry plum pulp or pomace is mixed with 50% to 95% ethanol according to the ratio of material to liquid: (2-10), treated at ultra-high pressure 200-600MPa for 3-10min, and filtered to obtain pulp Or pomace extract; In some embodiments, the solid-liquid ratio is selected as 1:5; In some embodiments, the ultra-high pressure is 400MPa; In some embodiments, the ultra-high pressure treatment time is selected for 5min; In some embodiments, the ethanol is 50% ethanol; Step 3: Red flesh apple and cherry plum pulp extract or the combined solution of fruit juice and pomace extract is concentrated by rotary evaporation at 30-60°C to obtain red flesh apple and cherry plum ultra-high pressure alcohol extract; In some embodiments, the rotary evaporation temperature is selected at 50° C.; optionally, the extract is evaporated to remove residual ethanol therein; Step 4: Mix the extracted red flesh apple and cherry plum UHP alcohol extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) to prepare a composition, or in step 1, mix the raw materials of red flesh apple and cherry plum according to the aforementioned different ratios, and then extract. Subcritical extraction (aka "subcritical extraction")

In certain embodiments, the red-fleshed apple and cherry plum extracts are obtained by subcritical extraction (eg, subcritical water extraction or subcritical alcohol extraction).

In certain embodiments, the subcritical extraction comprises the steps of: Step 1: Deseeding or pitting red apples and cherry plums, respectively, to obtain pulp directly, or to separate juice and pomace; in some embodiments, using gauze (such as 100 to 300 mesh gauze, such 200 mesh gauze) to filter and separate the juice and pomace; Step 2: Mix the pulp or pomace with an extraction medium (such as water or ethanol), perform subcritical extraction treatment (such as treatment for 10-50min, such as 30min), and filter to obtain pulp or pomace extract; in some embodiments , the pulp or pomace is mixed with the extraction medium in a weight ratio of 1:(2-10), such as 1:5; in certain embodiments, the ethanol is 50% to 95% ethanol; in some In an embodiment, the treatment temperature of the subcritical extraction is 100-150°C (for example, 110°C); Step 3: concentrating the combined pulp or juice and pomace extract to obtain subcritical extracts of red-fleshed apple and cherry plum; in some embodiments, concentrating by rotary evaporation; in some embodiments In the scheme, the temperature of the rotary evaporation is 30-60°C, such as 50°C; Step 4: Mix the extracted red flesh apple and cherry plum subcritical extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare a composition, or in step 1, mix the raw materials of red flesh apple and cherry plum according to the aforementioned different ratios, and then extract.

In certain embodiments, the red-fleshed apple and cherry plum extracts of the present invention are obtained by subcritical water extraction, and in certain embodiments, the subcritical water extraction comprises the steps of: Step 1: Deseeding or pitting the red-fleshed apples and cherry plums respectively, and beating to obtain pulp directly, or filtering the juices with 100 to 300 mesh gauze to obtain red-flesh apples and cherry plum juices, and separating the juice and pomace; In certain embodiments, the gauze has a mesh of 200 mesh; Step 2: Red meat apple and cherry plum pulp or pomace is mixed with water according to the ratio of material to liquid: (2-10), subcritical extraction is carried out for 10-50 minutes, and the treatment temperature is 100-150 ° C, and the pulp or pomace is obtained by filtration Pomace extract; in some embodiments, the ratio of solid to liquid is 1:5; in some embodiments, the treatment temperature is 110°C; in some embodiments, the treatment time is 30min; Step 3: Red flesh apple and cherry plum pulp extract or the combined liquid of juice and pomace extract is concentrated by rotary evaporation at 30-60°C to obtain red flesh apple and cherry plum subcritical water extract; In some embodiments, the rotary evaporation temperature is selected at 50°C; Step 4: Mix the extracted red-flesh apple and cherry plum subcritical water extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) to prepare a composition, or in step 1, mix the raw materials of red flesh apple and cherry plum according to the aforementioned different ratios, and then extract.

In certain embodiments, the red-fleshed apple and cherry plum extracts of the present invention are obtained by subcritical alcohol extraction, and in certain embodiments, the subcritical alcohol extraction comprises the steps of: Step 1: Deseeding or pitting the red-fleshed apples and cherry plums, respectively, and beating to obtain pulp directly, or filtering the juices with 100-300 mesh gauze to obtain red-flesh apples and cherry plum juices, and separating the juice and pomace; In certain embodiments, the gauze has a mesh of 200 mesh; Step 2: Red meat apple and cherry plum pulp or pomace is mixed with 50% to 95% ethanol according to the ratio of solid to liquid: (2-10), subcritical extraction for 10-50min, and the treatment temperature is 100-150°C. Filtration to obtain fruit pulp or pomace extract; in some embodiments, the ratio of solid to liquid is 1:5; in some embodiments, the treatment temperature is 110°C; in some embodiments, the treatment time is 30min ; Step 3: Red flesh apple and cherry plum pulp extract or the combined liquid of juice and pomace extract is concentrated by rotary evaporation at 30-60°C to obtain red flesh apple and cherry plum subcritical alcohol extract; In some embodiments, the rotary evaporation temperature is selected at 50° C.; optionally, the extract is evaporated to remove residual ethanol therein; Step 4: Mix the extracted red flesh apple and cherry plum subcritical alcohol extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) to prepare a composition, or in step 1, mix the raw materials of red flesh apple and cherry plum according to the aforementioned different ratios, and then extract.

As mentioned above, the product form of the composition of the present invention can be fresh fruit, fruit juice, jam, fruit powder (such as freeze-dried powder), candy (such as compressed candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, Wine or pastries. In addition, the product form of the composition of the present invention may be medicine, general food or health food. In some embodiments, in product forms such as fruit juice, jam, fruit powder (such as freeze-dried powder), candy (such as compressed candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or cake, the present invention The composition can be used as the main raw material, for example, accounting for more than 30wt%, more than 40wt%, more than 50wt%, more than 60wt%, more than 70wt%, more than 80wt%, more than 90wt% or 100wt% of the total raw materials; optionally, the product It may also contain other ingredients such as water, sweetening substances, or other kinds of fruits or their extracts.

Exemplary methods for preparing red-fleshed apple and cherry plum products (eg, fresh fruit, juice, jam, freeze-dried powder, compressed candy, oral liquid, capsule) are provided below. fresh fruit

In certain embodiments, red-fleshed apples and cherry plums can be obtained by a method comprising the steps of: Step 1: Harvest red-fleshed apples and cherry plums from one month before maturity to full maturity, and choose sunny days for fruit picking; Step 2: Pick and remove rotten or poorly colored fruits; Step 3: refrigerated storage after harvest; Step 4: Combine red flesh apples and fresh cherry plums according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1).

The obtained fresh apples with red flesh and cherry plums can be eaten directly. juice

In certain embodiments, red apple and cherry plum juices may be obtained by a process comprising the steps of: Step 1: Deseeding or pitting the red flesh apples and cherry plums respectively, and beating; Step 2: Filtrating the slurry (for example, filtering with gauze of 100 to 300 mesh) to obtain turbid fruit juice of red apple and cherry plum juice; in some embodiments, the mesh of the gauze is 200 mesh; Step 3: Mix red flesh apple and cherry plum juice in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare Composition, or in step 1 first mix the raw materials of red flesh apple and cherry plum according to the above-mentioned different ratios, and then make pulp to prepare fruit juice.

In some embodiments, red apple and cherry plum juices can also be obtained by a process comprising the steps of: Step 1: Deseeding or pitting the red flesh apples and cherry plums respectively, and beating; Step 2: filter the slurry (for example, filter with 100 to 300 mesh gauze), add pectinase to enzymatically filter; make red apple and cherry plum juice clear juice; in some embodiments, the mesh of the gauze 200 mesh; Step 3: Mix red flesh apple and cherry plum juice in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare Composition, or in step 1, firstly mix the raw materials of red flesh apple and cherry plum according to the aforementioned different ratios, and then perform beating to prepare fruit juice. jam

In certain embodiments, red apple and marmalade are obtainable by a method comprising the steps of: Step 1: Deseeding or pitting the red flesh apples and cherry plums respectively, and beating; Step 2: Boil and heat the pulp to make red apple and cherry plum jam; Step 3: Mix red apple and cherry plum jam in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare Composition, or in step 1 first mix the raw materials of red apples and cherry plums according to the aforementioned different ratios, and then beat to prepare jam. freeze-dried powder

In some embodiments, the freeze-dried powder of red-fleshed apple and cherry plum can be obtained by a method comprising the following steps: Step 1: Remove the seeds or pits of the red flesh apples and cherry plums respectively, and beat Step 2: Freeze-drying the pulp to make a freeze-dried pulp powder; Step 3: Mix red flesh apple and cherry plum freeze-dried powder according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) It is prepared into a composition, or in step 1, the raw materials of red-fleshed apples and cherry plums are mixed according to the aforementioned different ratios, and then beaten to prepare freeze-dried powder.

In some embodiments, the freeze-dried powder of red-flesh apple and cherry plum can also be obtained by a method comprising the following steps: Step 1: Deseeding or pitting the red flesh apples and cherry plums respectively, and beating; Step 2: filtering the slurry (for example, filtering with 100 to 300 mesh gauze) to obtain red flesh apple and cherry plum juice; in some embodiments, the gauze has a mesh of 200 mesh; Step 3: freeze-drying the fruit juice to make fruit juice freeze-dried powder; Step 4: Mix red flesh apple and cherry plum freeze-dried powder according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) It is prepared into a composition, or in step 1, the raw materials of red-fleshed apples and cherry plums are mixed according to the aforementioned different ratios, and then beaten to prepare freeze-dried powder. Tablet Candy

In certain embodiments, red apple and cherry plum candies are obtainable by a method comprising the steps of: Step 1: Deseeding or pitting the red flesh apples and cherry plums respectively, and beating; Step 2: Freeze-drying the pulp to make a freeze-dried pulp powder; Step 3: tableting the freeze-dried fruit pulp and auxiliary materials to make tablet candy; Step 4: Mix red apples and cherry plum candies in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) It is prepared into a composition, or in step 1, the raw materials of red-fleshed apples and cherry plums are first mixed according to the aforementioned different ratios, and then beating is performed to prepare tableted candies.

In some embodiments, red apple and cherry plum candies are also obtainable by a process comprising the steps of: Step 1: Deseeding or pitting the red flesh apples and cherry plums respectively, and beating; Step 2: filtering the slurry (for example, filtering with gauze of 100 to 300 mesh) to obtain apple juice with red flesh; in some embodiments, the mesh of the gauze is 200 mesh; Step 3: freeze-drying the fruit juice to make fruit juice freeze-dried powder; Step 4: Tablet the freeze-dried fruit juice powder and auxiliary materials to make tablet candy. Step 5: Mix red apples and cherry plum candies in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) It is prepared into a composition, or in step 1, the raw materials of red-fleshed apples and cherry plums are first mixed according to the aforementioned different ratios, and then beating is performed to prepare tableted candies. oral liquid

In some embodiments, the red-fleshed apple and cherry plum oral liquid can be obtained by a method comprising the following steps: Step 1: Prepare red-fleshed apple and cherry plum extracts (e.g. , prepared by leaching extraction, enzymatic ultrasonic extraction, ultra-high pressure extraction or subcritical extraction, the steps are the same as above); Step 2: Sterilize the red-flesh apple and cherry plum extracts to prepare the red-flesh apple oral liquid.

In some embodiments, the red-flesh apple and cherry plum oral liquid can also be obtained by a method comprising the following steps: Step 1: Prepare red-fleshed apple and cherry plum extracts (e.g. , prepared by leaching extraction, enzymatic ultrasonic extraction, ultra-high pressure extraction or subcritical extraction, the steps are the same as above); Step 2: adding pectinase to enzymatically hydrolyze and filter to prepare clear juice extract composition; Step 3: Sterilize the extracts of red-flesh apple and cherry plum juice to prepare red-flesh apple oral liquid. capsule

In certain embodiments, red-fleshed apple and cherry plum capsules are obtainable by a method comprising the steps of: Step 1: Deseeding or pitting the red flesh apples and cherry plums respectively, and beating; Step 2: Freeze-drying the pulp to make a freeze-dried pulp powder; Step 3: Mix red flesh apple and cherry plum freeze-dried powder according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) Prepare a composition, or mix the raw materials of red-fleshed apples and cherry plums according to the aforementioned different ratios in step 1, and then perform beating to prepare freeze-dried powder; Step 4: Put the freeze-dried fruit pulp powder into the capsule shell to make a capsule.

In some embodiments, red flesh apple and cherry plum capsules can also be obtained by a method comprising the following steps: Step 1: Deseeding or pitting the red flesh apples and cherry plums respectively, and beating; Step 2: filtering the slurry (for example, filtering with 100 to 300 mesh gauze) to obtain red flesh apple and cherry plum juice; in some embodiments, the gauze has a mesh of 200 mesh; Step 3: freeze-drying the fruit juice to make fruit juice freeze-dried powder; Step 4: Mix red flesh apple and cherry plum freeze-dried powder according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) Prepare a composition, or mix the raw materials of red-fleshed apples and cherry plums according to the aforementioned different ratios in step 1, and then perform beating to prepare freeze-dried powder; Step 5: The fruit juice freeze-dried powder is packed into a capsule shell to make a capsule. Beneficial effect

The present invention selects natural red-flesh apples and cherry plums, and the prepared combination product of red-flesh apples and cherry plums has a good hypoglycemic effect. effect. The effect of the combined use of the composition and the medicine is better than that of the composition or the medicine alone.

Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. Example 1:

The red-flesh apple and cherry plum products described in this embodiment refer to red-flesh apple and cherry plum fresh fruit.

Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) are harvested from one month before maturity to full maturity. When picking fruits, it is preferred to pick fruits on sunny days, select and remove rotten or poor-colored fruits, and harvest them. Then store in the refrigerator. The obtained red flesh apples and cherry plums are combined according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1), and the fresh fruits can be used directly edible. Example 2:

The red-fleshed apple and cherry plum products described in this embodiment refer to red-flesh apple and cherry plum juice cloudy juice.

Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) were harvested and then pulped after deseeding or pitting. The juice was filtered with 200-mesh gauze to obtain turbid juice from red-fleshed apples and cherry plums. Red meat apple and cherry plum cloudy juice are mixed according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare a composition , or first mix the raw materials of red apples and cherry plums according to the aforementioned different ratios, and then beat to prepare juice. Example 3:

The red-flesh apple and cherry plum products described in this embodiment refer to the clear juice of red-flesh apple and cherry plum juice.

Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) were harvested and then pulped after deseeding or pitting. The juice was filtered with 200-mesh gauze to obtain turbid juice from red-fleshed apples and cherry plums. Add pectinase to hydrolyze and filter to obtain clear juice. The composition is prepared by mixing red-fleshed apples and cherry-plum juice in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1), Or firstly mix red meat apples and cherry plum raw materials according to the above-mentioned different ratios, and then make a beating to prepare fruit juice. Example 4:

The red-flesh apple and cherry plum products described in this embodiment refer to red-flesh apple and cherry plum jam.

Red-fleshed apples (Hong Xun No. 1) and cherry plums (black fruit) are deseeded or pitted, then pulped, boiled and heated to make a thicker jam. Red flesh apple and cherry plum jam are mixed according to different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare a composition, Or mix the raw materials of red-fleshed apples and cherry plums according to the above-mentioned different ratios, and then make a beating to prepare jam. Example 5:

The red-fleshed apple and cherry plum products described in this embodiment refer to the freeze-dried powder of red-flesh apple and cherry plum.

Red-fleshed apples (Hongxun No. 1) and cherry plums (Heiguo) were pulped after deseeding or pitting, and freeze-dried to make pulp freeze-dried powder. Mix red flesh apple and cherry plum freeze-dried powder according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare a combination or mix the raw materials of red-fleshed apples and cherry plums according to the above-mentioned different ratios, and then perform beating to prepare freeze-dried powder. Embodiment 6:

The red-flesh apple and cherry plum products described in this embodiment refer to red-flesh apple and cherry plum sliced candies.

Red-fleshed apples (Hong Xun No. 1) and cherry plums (black fruit) are deseeded or pitted, then pulped, freeze-dried to make pulp freeze-dried powder, and the pulp freeze-dried powder and auxiliary materials are compressed to make tablet candy. Mix red apples and cherry plum candies in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare a combination or mix the raw materials of red flesh apple and cherry plum according to the aforementioned different proportions, and then beat the pulp to prepare tablet candy. Embodiment 7:

The red-flesh apple and cherry plum products described in this embodiment refer to the oral liquid of red-flesh apple and cherry plum water extraction. (1) Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) were deseeded or pitted and then pulped to obtain red-flesh apple and cherry plum pulp. (2) Add water to the pulp of red-fleshed apples and cherry plums according to the ratio of solid to liquid 1:5, extract with water at 50°C for 60 minutes, and filter to obtain the pulp extract. (3) The extract was concentrated by rotary evaporation at 50°C to obtain water extracts of red flesh apple and cherry plum. (4) Mix the extracted red flesh apple and cherry plum extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10: 1) Prepared into a composition. (5) The oral liquid was prepared by sterilizing the combined extracts of red-flesh apple and cherry plum. Embodiment 8:

The red-flesh apple and cherry plum products described in this example refer to red-flesh apple (red love) and cherry plum (red fruit) water extraction oral liquid. The preparation steps are the same as in Example 7. Embodiment 9:

The red-flesh apple and cherry plum products described in this example refer to the oral liquid of red-flesh apple (Hongxun No. 1) and cherry plum (black fruit) alcohol extraction.

Refer to the steps (1) to (5) of Example 7, wherein the fruit pulp is added with 50% ethanol at a ratio of 1:5 to material. The alcoholic extract was evaporated to dryness with ethanol. Example 10:

The red-flesh apple and cherry plum products described in this embodiment refer to the oral liquid of red-flesh apple and cherry plum enzymatically hydrolyzed with ultrasonic water extraction. (1) Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) were deseeded or pitted and then pulped to obtain red-flesh apple and cherry plum pulp. (2) Red flesh apple and cherry plum pulp are prepared by adding water at a material-to-liquid ratio of 1:5, adding 0.5% (based on the weight of the pulp) cellulase, extracting in a water bath at 45°C for 60 minutes, ultrasonic wave at 500W for 30 minutes, and filtering. Fruit pulp extract. (3) The extract was concentrated by rotary evaporation at 50°C to obtain enzymolysis ultrasonic water extracts of red flesh apple and cherry plum. (4) Mix the extracted red flesh apple and cherry plum extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10: 1) Prepared into a composition. (5) The oral liquid was prepared by sterilizing the combined extracts of red-flesh apple and cherry plum. Example 11:

The red-flesh apple and cherry plum products described in this example refer to red-flesh apple (Hongxun No. 3) and cherry plum (red fruit) enzymatically hydrolyzed ultrasonic water extraction oral liquid. The preparation steps are the same as in Example 10. Example 12:

The red-flesh apple and cherry plum products described in this example refer to the oral liquid of red-flesh apple (Hongxun No. 1) and cherry plum (black fruit) enzymolysis ultrasonic alcohol extraction.

Refer to the steps (1) to (5) of Example 10, wherein the pulp is added 50% according to the ratio of material to liquid: 1:5. The alcoholic extract was evaporated to dryness with ethanol. Example 13:

The red-fleshed apple and cherry plum products described in this embodiment refer to the ultra-high pressure water extraction oral liquid of red-flesh apple and cherry plum. (1) Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) were deseeded or pitted and then pulped to obtain red-flesh apple and cherry plum pulp. (2) Add water to the pulp of red-fleshed apples and cherry plums at a material-to-liquid ratio of 1:5, process at an ultra-high pressure of 400Mpa for 5 minutes, and filter to obtain pulp extracts. (3) The extract was concentrated by rotary evaporation at 50°C to obtain ultra-high pressure water extracts of red flesh apple and cherry plum. (4) Mix the extracted red flesh apple and cherry plum extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10: 1) Prepared into a composition. (5) The oral liquid was prepared by sterilizing the combined extracts of red-flesh apple and cherry plum. Example 14:

The red-fleshed apple and cherry plum products described in this example refer to red-flesh apple (Hong Xun No. 6) and cherry plum (red fruit) ultra-high pressure water extraction oral liquid. The preparation steps are the same as in Example 13. Example 15:

The red-flesh apple and cherry plum products described in this example refer to the ultra-high pressure alcohol extraction oral liquid of red-flesh apple (Hongxun No. 1) and cherry plum (black fruit).

Refer to the steps (1) to (5) of Example 13, in which the pulp is added 50% according to the ratio of material to liquid: 1:5. The alcoholic extract was evaporated to dryness with ethanol. Example 16:

The red-fleshed apple and cherry plum products described in this embodiment refer to the subcritical water extraction oral liquid of red-flesh apple and cherry plum. (1) Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) were deseeded or pitted and then pulped to obtain red-flesh apple and cherry plum pulp. (2) Red flesh apple and cherry plum pulp were added with water according to the ratio of solid to liquid 1:5, subcritical extraction was carried out for 30 minutes, the treatment temperature was 110°C, and the pulp extract was obtained by filtration. (3) The extract was concentrated by rotary evaporation at 50°C to obtain subcritical water extracts of red-fleshed apple and cherry plum. (4) Mix the extracted red flesh apple and cherry plum extracts in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10: 1) Prepared into a composition. (5) The oral liquid was prepared by sterilizing the combined extracts of red-flesh apple and cherry plum. The hypoglycemic effect evaluation of embodiment product: Experimental example 1: Evaluation of the hypoglycemic effect of zebrafish (1) Test method

Zebrafish juveniles with an embryonic development phase of 5 dpf were selected and divided into model group, control group, positive drug group (metformin) and sample group. The model group, the positive drug group and the sample group were soaked in glucose solution and fed with high-fat feed; the control group was cultured with system water and fed with high-fat feed. Feed the juveniles 2 times a day. The positive drug group was given metformin, and the sample group was given the test sample. At 9dpf, collect juvenile fish, add water after grinding, and measure the glucose content in the total interstitial fluid with a blood glucose meter. Blood sugar suppression rate (%) = (model group blood sugar - drug group blood sugar) / model group blood sugar × 100 (2) Test results

The test results are shown in Table 1 below. In Table 1 below, the samples of the red-flesh apple group and the cherry plum group are the red-flesh apple enzymatically hydrolyzed ultrasonic water extract and cherry plum enzymatically hydrolyzed ultrasonic water extract prepared in step (3) of Example 10, respectively. The samples of the composition group of red-flesh apple and cherry plum are compositions of various weight ratios prepared in step (4) of Example 10. In addition, the administration concentration corresponding to each group is shown by a single bottom line, in which: 1mg/mL or 0.5mg/mL represents the concentration of the active drug dispersed in water; 0.01% represents the enzymatic hydrolysis ultrasonic water extract of red meat apple, or cherry plum Concentration of enzymatic hydrolysis ultrasonic water extract, or composition composed of red flesh apple enzymatic hydrolysis ultrasonic water extract and cherry plum enzymatic ultrasonic water extract in different weight ratios dispersed in water.

Afterwards, with reference to "CN104127427B An Anti-cancer Synergistic Composition" and the document "The Synergistic Effect of Curcumin and Adriamycin in Combined Application of Anti-tumor", the interaction coefficient q=E(A+B)/(EA+(1-EA ) × EB). Among them, E(A + B) is the inhibition rate of the two drugs combined, and EA and EB are the inhibition rates of the two drugs alone. A value of q between 0.85 and 1.15 means that the effects are additive, q>1.15 means that the effects are enhanced and there is a synergistic effect, and q<0.85 means that the effects of the two drugs are antagonistic. The calculation results are shown in Table 1 below. Table 1 Hypoglycemic effect on zebrafish of red meat apple and cherry plum composition (Example 10) sample Blood sugar value mmol/L Blood sugar suppression rate% q value Model 8.325 Metformin 1mg/mL 5.675* 31.83 / Metformin 0.5mg/mL 6.961* 16.38 / Red flesh apple 0.01% 6.400* 23.12 / Cherry plum 0.01% 6.767* 18.71 / Red flesh apple: cherry plum 10:1 (0.01%) 4.631** 44.37 1.18 Red flesh apple: cherry plum 5:1 (0.01%) 4.712** 43.40 1.16 Red flesh apple:cherry plum 2:1 (0.01%) 4.623** 44.47 1.19 Red flesh apple: cherry plum 1:1 (0.01%) 4.570** 45.11 1.20 Red flesh apple: cherry plum 1:2 (0.01%) 4.386** 47.32 1.26 Red flesh apple: cherry plum 1:5 (0.01%) 4.650** 44.14 1.18 Red flesh apple: cherry plum 1:10 (0.01%) 4.734** 43.14 1.15 Red flesh apple + cherry plum 1:1 (0.01%) + Metformin 1mg/mL 3.428*** 58.82 0.93 Red flesh apple + cherry plum 1:1 (0.01%) + Metformin 0.5mg/mL 3.520*** 57.72 1.06 The significance is compared with the model group; * p<0.05, ** p<0.01, *** p<0.001

It can be seen from the above table 1 that the blood sugar suppression rate of the combination of red flesh apple and cherry plum (10:1 to 1:10) is higher than that when used alone, and the calculated q values are all greater than 1.15, indicating that it has a synergistic effect.

In addition, the composition of red-flesh apple and cherry plum has an additive effect (0.85<q<1.15) when used in combination with the active drug. The hypoglycemic effect of the combination of the composition and the active drug is better than that of the single use. Combined use with lower doses of active drugs can significantly improve the effect. Experimental example 2: Evaluation of hypoglycemic effect in mice (1) Test method

Healthy db/db mice were taken and divided into positive drug group (metformin, dapagliflozin), model group and sample group; in addition, there was a normal control group, which was C57 wild-type mice with BKS background; Group 20. After adaptive feeding, the model group was given distilled water (0.5mL/rat), the positive drug group was given metformin or dapagliflozin, and the sample group was given samples (0.5mL/rat), and the experimental period was 14 days. After blood was taken from the tip of the tail, the glucose content in the mouse blood was measured with a blood glucose meter. Blood sugar suppression rate (%) = (blood sugar value of model group - blood sugar value of drug group) / blood sugar value of model group × 100 (2) Test results

The test results are shown in Table 2 below. In Table 2 below, the samples of the red-flesh apple group and the cherry plum group are the red-flesh apple enzymatically hydrolyzed ultrasonic water extract and cherry plum enzymatically hydrolyzed ultrasonic water extract prepared in step (3) of Example 10, respectively. The samples of the composition group of red-flesh apple and cherry plum are compositions of various weight ratios prepared in step (4) of Example 10. In addition, the corresponding administered doses for each group are shown with a single bottom line.

Afterwards, with reference to "CN104127427B An Anti-cancer Synergistic Composition" and the document "The Synergistic Effect of Curcumin and Adriamycin in Combined Application of Anti-tumor", the interaction coefficient q=E(A+B)/(EA+(1-EA ) × EB). Among them, E(A + B) is the inhibition rate of the two drugs combined, and EA and EB are the inhibition rates of the two drugs alone. A value of q between 0.85 and 1.15 means that the effects are additive, q>1.15 means that the effects are enhanced and there is a synergistic effect, and q<0.85 means that the effects of the two drugs are antagonistic. The calculation results are shown in Table 2 below. Table 2 The hypoglycemic effect of the red meat apple and cherry plum composition (Example 10) on mice sample Blood sugar value mmol/L Blood sugar suppression rate% q value Model 22.9 Metformin 300mg/kg 10.6 53.72 / Metformin 150mg/kg 15.1 34.06 / Dapagliflozin 3 mg/kg 9.5 58.52 / Dapagliflozin 1.5 mg/kg 16.4 28.38 / Red meat apple 20g/kg 15.5 32.31 / Cherry plum 20g/kg 15.7 31.44 / Red flesh apple: cherry plum 10:1 20g/kg 8.7 62.01 1.16 Red flesh apple: cherry plum 5:1 20g/kg 8.5 62.88 1.17 Red flesh apple: cherry plum 2:1 20g/kg 8.1 64.63 1.21 Red flesh apple: cherry plum 1:1 20g/kg 8.5 62.88 1.17 Red flesh apple: cherry plum 1:2 20g/kg 8.4 63.32 1.18 Red flesh apple: cherry plum 1:5 20g/kg 8.2 64.19 1.20 Red flesh apple: cherry plum 1:10 20g/kg 8.7 62.01 1.16 Red flesh apple + cherry plum 1:1 (20g/kg) + Metformin 300mg/kg 6.5 71.62 0.86 Red flesh apple + cherry plum 1:1 (20g/kg) + metformin 150mg/kg 7.8 65.94 0.87 Red flesh apple + cherry plum 1:1 (20g/kg) + dapagliflozin 3mg/kg 6.2 72.93 0.86 Red flesh apple + cherry plum 1:1 (20g/kg) + Dapagliflozin 1.5mg/kg 7.6 66.81 0.91 The significance is compared with the model group; * p<0.05, ** p<0.01, *** p<0.001

It can be seen from the above table 2 that the blood sugar suppression rate of the combination of red flesh apple and cherry plum (10:1 to 1:10) is higher than that when used alone, and the calculated q values are all greater than 1.15, indicating that it has a synergistic effect. In addition, the composition of red-flesh apple and cherry plum has an additive effect (0.85<q<1.15) when used in combination with the active drug.

The above examples are only used to illustrate the technical solution of the present invention rather than limit it. Any modification or equivalent replacement of the technical solution of the present invention without departing from the purpose and scope of the technical solution of the present invention shall be covered by the protection scope of the present invention within.

Claims (9)

A use of a composition in the preparation of medicine, common food or health food, the medicine, common food or health food is used for lowering blood sugar or for preventing and/or treating a blood sugar disease, wherein the composition comprises: a first A component, the first component is a red apple extract; and a second component, the second component is a cherry plum extract; wherein, the first component and the second component The weight ratio is 1:10 to 10:1; wherein, the red-fleshed apple ( Malus neidzwetzkyana Dieck) is a plant of the genus Malus Mill of the family Rosaceae, and the cherry plum ( Prunus cerasifera Ehrhart) is a plant of the genus Prunus of the family Rosaceae; wherein The red-flesh apple extract and the cherry plum extract are obtained by enzymolysis ultrasonic water extraction, and the enzymolysis ultrasonic water extraction includes the following steps: Step 1: deseeding the red-flesh apple and the cherry plum respectively Beating after pitting to obtain fruit pulp directly, or filter the juice with 100 to 300 mesh gauze to obtain red flesh apple and cherry plum juice, and separate juice and pomace; Step 2: Put red flesh apple and cherry plum juice or fruit juice The residue is mixed with water according to the ratio of material to liquid 1: (2-10), and 0.2% to 1% cellulase is added based on the weight of pomace or fruit pulp, extracted in a water bath at 30-50°C for 30-100min, and supercharged at 100-500W Sonic for 20-60 minutes, filter to obtain fruit pulp or pomace extract; Step 3: Red flesh apple and cherry plum pulp or the combined solution of fruit juice and pomace extract, concentrate by rotary evaporation at 30-60°C to obtain red Enzymatic Sonication Water Extract of Flesh Apple and Enzymatic Sonication Water Extract of Cherry Plum. The use according to claim 1, wherein the blood sugar disease is selected from diabetes and diabetes-related diseases. The use as described in Claim 1, wherein the composition exists in the form of fresh fruit, fruit juice, jam, fruit powder, candy, oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or cake. Use as described in claim item 1, wherein, the red meat apple is selected from: Hongxun No. 1 (Hongxun No. 1), Hongxun No. 3 (Hongxun No. 3), Hongxun No. 6 (Hongxun No. 6) , Xia Hongrou, Dai Hong, Red Love, Roberts Crab, Red Dream, Ruby Sweet; or, the cherry plum is selected from Cherry Plum Red Fruit, Cherry Plum Black Fruit, Cherry Plum Purple Fruit, Cherry Plum Purple Black Fruit, Cherry Plum Yellow fruit. A combination product, which includes: a first product, the first product is the composition defined in any one of claim 1 to claim 4; a second product, the second product is a preventive and/or therapeutic Drugs for blood sugar disorders. The combination product as described in claim 5, wherein, the blood sugar disease is selected from diabetes and diabetes-related diseases; or, the drug for preventing and/or treating blood sugar disease is selected from sulfonylurea drugs, biguanide drugs, α- Glycosidase inhibitors, insulin sensitizers, insulin secretagogues, DPP-4 inhibitors, SGLT-2 inhibitors, insulin. The combined product according to claim 5, wherein the weight ratio of the first product to the second product is 20000:0.75 to 20000:600. A use of a combination product as described in any one of claim 5 to claim 7 in the preparation of a medicine, common food or health food, the medicine, common food or health food is used for lowering blood sugar, or for preventing and /or to treat a blood sugar disorder. The use according to claim 8, wherein the blood sugar disease is selected from diabetes and diabetes-related diseases.