CN115152531A - Production method of Dictyophora rubrovalvata glass bottle mother seeds - Google Patents
Production method of Dictyophora rubrovalvata glass bottle mother seeds Download PDFInfo
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- CN115152531A CN115152531A CN202210961419.8A CN202210961419A CN115152531A CN 115152531 A CN115152531 A CN 115152531A CN 202210961419 A CN202210961419 A CN 202210961419A CN 115152531 A CN115152531 A CN 115152531A
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- 239000011521 glass Substances 0.000 title claims abstract description 36
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 23
- 241001313734 Dictyophora Species 0.000 title claims abstract description 22
- 239000001963 growth medium Substances 0.000 claims abstract description 59
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 40
- 235000016068 Berberis vulgaris Nutrition 0.000 claims abstract description 31
- 241000335053 Beta vulgaris Species 0.000 claims abstract description 31
- 235000018185 Betula X alpestris Nutrition 0.000 claims abstract description 28
- 235000018212 Betula X uliginosa Nutrition 0.000 claims abstract description 28
- 239000000843 powder Substances 0.000 claims abstract description 28
- 239000002994 raw material Substances 0.000 claims abstract description 24
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 21
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 20
- 229930006000 Sucrose Natural products 0.000 claims abstract description 20
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 20
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 20
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 20
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 20
- 229910052602 gypsum Inorganic materials 0.000 claims abstract description 18
- 239000010440 gypsum Substances 0.000 claims abstract description 18
- 235000019764 Soybean Meal Nutrition 0.000 claims abstract description 13
- 239000004455 soybean meal Substances 0.000 claims abstract description 13
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 11
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 11
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 7
- 244000068988 Glycine max Species 0.000 claims abstract description 7
- 238000011081 inoculation Methods 0.000 claims description 50
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 241000890685 Dictyophora rubrovolvata Species 0.000 claims description 19
- 239000000463 material Substances 0.000 claims description 18
- 238000003756 stirring Methods 0.000 claims description 15
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 239000000126 substance Substances 0.000 claims description 13
- 238000001816 cooling Methods 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 235000013601 eggs Nutrition 0.000 claims description 6
- 238000004080 punching Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000002361 compost Substances 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 3
- 238000011218 seed culture Methods 0.000 claims description 3
- 239000002023 wood Substances 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 23
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 210000003813 thumb Anatomy 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
- A01G18/68—Cultivation bottles
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for producing Dictyophora rubrovalvata glass bottle mother strain, which comprises a novel culture medium, wherein the raw materials of the culture medium comprise birch sawdust, wheat bran powder, soybean powder, beet pulp, white sugar, gypsum, potassium dihydrogen phosphate and magnesium sulfate. The raw materials of the culture medium comprise, by weight, 70-80 parts of birch sawdust, 10-20 parts of wheat bran powder, 2-8 parts of soybean meal, 3-6 parts of beet pulp, 0.5-2.0 parts of white sugar, 0.5-2.5 parts of gypsum, 0.2-0.8 part of monopotassium phosphate and 0.2-1.0 part of magnesium sulfate. The production method of the invention obviously improves the amount of the single inoculable strain, improves the working efficiency, reduces the infection rate of the stock seeds, and improves the number of the inoculable stock seeds in a single glass bottle by more than 5 times compared with the stock seeds in the traditional test tube.
Description
Technical Field
The invention belongs to the technical field of Dictyophora rubrovalvata production, and particularly relates to the technical field of a production method of a Dictyophora rubrovalvata glass bottle mother strain.
Background
The prior art is as follows: publication No. CN110367047A, application date 2019, 6 and 3 months, invention name: a Dictyophora rubrovalvata test tube mother strain culture medium and a Chinese patent application applying the same. The culture medium provided by the patent application is very suitable for the production of Dictyophora rubrovalvata test tube stock seeds, but the test tube stock seeds are generally 50ml and 100ml, and the production mode adopting the test tube stock seeds has the following technical defects: 1) Compared with the same inoculation procedure test by using the same batch of people, the test tube mother seeds have less strain amount, each test tube mother seed can only be transferred to 4 bottles of stock seeds, 250 test tube mother seeds are needed for inoculating 1000 bottles of stock seeds, the operation is complicated, and the workload is large; 2) The test tube mother seeds have poor tightness and are easy to crack; 3) The pollution rate of the mother seeds adopting the test tubes is high; 4) The growth of hyphae is not easy to observe. Therefore, the tube replacement mode brings new technical problems after a new production mode is provided; and after the test tube is replaced, the raw material formula of the culture medium needs to be adjusted, because: 1) The Dictyophora rubrovalvata strain in the prior art grows slowly after growing in a test tube; 2) When the mother clock is transferred, the mother seeds which grow most strongly are difficult to take out from the test tube without damage due to narrow space; 3) Due to the increased space, the mother clock culture medium after environmental changes needs to be adjusted to better adapt and grow the dictyophora rubrovolvata mother seeds. In conclusion, a new method for producing Dictyophora rubrovalvata mother strains needs to be researched.
Disclosure of Invention
The invention aims to solve the defects of the problems and provides a method for producing Dictyophora rubrovalvata glass bottle mother strains.
The invention is realized by adopting the following technical scheme.
A culture medium for producing Dictyophora rubrovalvata mother strain comprises birch sawdust, wheat bran powder, soybean powder, beet residue (providing sugar with good water binding capacity), white sugar, gypsum Fibrosum, potassium dihydrogen phosphate, and magnesium sulfate.
Further, in the raw materials of the culture medium, by weight, 70-80 parts of birch sawdust, 10-20 parts of wheat bran powder, 2-8 parts of soybean meal, 3-6 parts of beet pulp, 0.5-2.0 parts of white sugar, 0.5-2.5 parts of gypsum, 0.2-0.8 parts of potassium dihydrogen phosphate and 0.2-1.0 parts of magnesium sulfate.
Further, in the raw materials of the culture medium, by weight, 74 parts of birch sawdust, 15 parts of wheat bran powder, 4 parts of soybean meal, 4 parts of beet pulp, 1.5 parts of white sugar, 1 part of gypsum, 0.3 part of monopotassium phosphate and 0.2 part of magnesium sulfate.
Furthermore, in the raw materials of the culture medium, by weight, the birch sawdust is 80 parts, the wheat bran powder is 10 parts, the soybean meal is 2 parts, the beet pulp is 6 parts, the white sugar is 0.5 part, the gypsum is 0.5 part, the potassium dihydrogen phosphate is 0.2 part, and the magnesium sulfate is 1.0 part.
Further, in the raw materials of the culture medium, by weight, 70 parts of birch sawdust, 20 parts of wheat bran powder, 8 parts of soybean meal, 3 parts of beet pulp, 2.0 parts of white sugar, 2.5 parts of gypsum, 0.8 part of monopotassium phosphate and 0.2 part of magnesium sulfate.
Furthermore, water is added into the culture medium on the basis of raw materials, and the water content of the culture medium is controlled to be 65-70%.
The production method of Dictyophora rubrovalvata glass bottle stock culture by using any one of the culture media comprises the following steps:
step 1) preparing a culture medium material required for producing Dictyophora rubrovalvata mother strains according to the mixture ratio requirement;
step 2) calculating the use amount of each raw material component of the produced mother culture medium, diluting the soluble substances by using a small basin, and pouring the diluted solution into a container filled with 80 percent of the calculated total water amount for secondary dilution;
uniformly scattering insoluble substances into a stirrer, performing dry stirring for 20 minutes to obtain a dry stirring material, finally adding the diluted solution into the dry stirring material, and stirring for 30 minutes, wherein water is continuously added during stirring, and the water content of the culture medium is controlled to be 65-70%; obtaining a culture material;
step 3) filling the compost into a glass bottle according to the standard of loosening and tightening, filling sawdust to two centimeters away from the bottle opening, pressing the surface to be flat, filling a punching rod for punching, cleaning the glass bottle opening, sealing the glass bottle opening, and then boxing and sterilizing;
and (3) sterilizing requirements: (1) the temperature of the sterilizer must reach more than 100 ℃ within 1 hour; (2) the high pressure lasts for 3 hours at 121 ℃, and the normal pressure lasts for 11 hours above 100 ℃; (3) after sterilization, naturally reducing the temperature in the sterilizer to 60 ℃, slowly opening a sterilizer door to 20-30 cm (preventing external cold air from adsorbing bacteria sticks to form water drops), carrying the original seed bottles to a cooling chamber after waiting for one hour, and carrying out next inoculation after cooling to 20 +/-2 ℃;
step 4), sterilizing and disinfecting the inoculation tool, wherein the whole inoculation environment is carried out in a sterile environment;
step 5) cutting the selected robust dictyophora rubrovolvata eggs by using an inoculation knife, and taking two stipe (high cell activity) parts in the dictyophora rubrovolvata eggs as inoculation blocks; inserting the inoculation block into a glass bottle for inoculation; the size of the inoculation block is controlled to be 1.5 cm (the size of a thumb nail cover), after inoculation, a rubber plug is wiped by alcohol, and then the opening is sealed;
and 6) uniformly placing the inoculated mother seeds in a culture chamber for mother seed culture.
Further, the soluble substances are white sugar, monopotassium phosphate and magnesium sulfate.
Further, the insoluble substances are birch sawdust, wheat bran powder, soybean powder, beet pulp and gypsum.
Furthermore, the inoculation block is inserted into the glass bottle for inoculation, namely, the inoculation block is obliquely inserted into the culture material from the bottle mouth at the positions 1 cm and 3 cm downwards and is close to the bottle surface for inoculation (the inoculation is convenient for observing the later growth condition at the bottle surface, and the original seeds can grow rapidly by adopting a two-point inoculation method, so that the growth period is shortened).
Two-point inoculation method: the inoculation is carried out at the position 1 cm and 3 cm below the culture medium and close to the bottle surface, namely a two-point inoculation method.
The invention has the beneficial effects that 1) the glass bottle adopted by the invention replaces a test tube to become a new production carrier of Dictyophora rubrovalvata mother seeds, the capacity of the glass bottle is 500ml, the inoculation quantity is 20 times of that of the traditional test tube mother seeds, the single inoculation quantity is improved compared with the traditional test tube mother seeds, the inoculation quantity of the single glass bottle mother seeds is improved by more than 5 times compared with the traditional test tube mother seeds, and the working efficiency is obviously improved; 2) Compared with the same inoculation program test by using the same batch of people, the strain quantity of the mother seeds in the glass bottles is large, 20 bottles of original seeds can be inoculated in each bottle, and the pollution rate is greatly reduced; 3) The sealing performance of the glass bottle is far higher than that of a test tube mother seed; the sterilization process is matched with the sterilization device, so that the infection rate of the mother seeds is greatly reduced; 4) The obtaining mode of the inoculation block is far more scientific and reasonable than that of the inoculation block in a test tube, and the inoculation block is obliquely inserted at the positions 1 cm and 3 cm below the culture material and is inoculated at the position close to the bottle surface; not only the strain which grows strongly is satisfied, but also the requirement of later observation is satisfied; 5) Because of the spatial change of the growth environment of the mother seeds, the raw material formula of the culture medium needs to be prepared to adapt, the newly selected beet pulp has two beneficial effects of a, providing sugar and having good water retention, so the invention improves the water content; b. the beet pulp can be well matched with the birch sawdust, the beet pulp and the birch sawdust have adaptation of higher density and lower density in a physical state, and the beet pulp and the birch sawdust can better provide nutrient substances for Dictyophora rubrovolvata after being mixed in a biological property without generating adverse effects; 6) The newly used bottle body has a small mouth and a big belly, the culture materials are required to be filled into the glass bottle according to the loose and tight standards in the operation method of the invention, and the oxygen concentration from the bottle mouth to the bottom of the bottle bottom in the bottle can be controlled through the arrangement; further adjusting the comprehensive adaptation of the strain, the dictyophora rubrovolvata strain and the culture medium to be optimal; 7) The newly researched culture medium has the advantages of waste utilization and environmental protection.
The invention is further explained below with reference to the drawings and the detailed description.
Drawings
FIG. 1 is a diagram showing a glass bottle of the present invention in a use state.
FIG. 2 is one of the growth vigor diagrams of Dictyophora rubrovolvata glass bottle mother strains produced by using the culture medium and the production method of the present invention. (mycelium has grown to half flask)
FIG. 3 is the second growth chart of Dictyophora rubrovolvata glass bottle stock seeds produced by using the culture medium and the production method of the invention. (mycelium had grown to almost all of the bottles)
FIG. 4 is a growth chart of Dictyophora rubrovolvata test tube stock seeds produced by using a culture medium and a production method in the prior art.
Detailed Description
See fig. 1, 2, 3, 4.
A culture medium for producing Dictyophora rubrovalvata mother strain comprises birch sawdust, wheat bran powder, soybean powder, beet residue (providing sugar with good water binding capacity), white sugar, gypsum Fibrosum, potassium dihydrogen phosphate, and magnesium sulfate.
Further, in the raw materials of the culture medium, by weight, 70-80 parts of birch sawdust, 10-20 parts of wheat bran powder, 2-8 parts of soybean meal, 3-6 parts of beet pulp, 0.5-2.0 parts of white sugar, 0.5-2.5 parts of gypsum, 0.2-0.8 parts of monopotassium phosphate and 0.2-1.0 parts of magnesium sulfate.
Further, in the raw materials of the culture medium, by weight, 74 parts of birch sawdust, 15 parts of wheat bran powder, 4 parts of soybean meal, 4 parts of beet pulp, 1.5 parts of white sugar, 1 part of gypsum, 0.3 part of monopotassium phosphate and 0.2 part of magnesium sulfate. The embodiment is the best embodiment, no visible residue exists in the culture medium after the culture medium is used at the later stage, and the quality of the cultured Dictyophora rubrovolvata mother strain is the best.
Furthermore, in the raw materials of the culture medium, by weight, the birch sawdust is 80 parts, the wheat bran powder is 10 parts, the soybean meal is 2 parts, the beet pulp is 6 parts, the white sugar is 0.5 part, the gypsum is 0.5 part, the potassium dihydrogen phosphate is 0.2 part, and the magnesium sulfate is 1.0 part. In this embodiment, the weight ratio of the birch sawdust is increased, and the weight parts of the beet pulp are mainly adjusted, so as to adjust the beet pulp: the maximum value of the birch sawdust under the fixed value is tested and researched, if the weight part of the beet pulp is continuously increased, a certain amount of macroscopic white spot residues exist in the culture medium after the culture medium is used at the later stage, the main component of the residues is the beet pulp after testing, and if the raw material is wasted.
Further, in the raw materials of the culture medium, by weight, 70 parts of birch sawdust, 20 parts of wheat bran powder, 8 parts of soybean meal, 3 parts of beet pulp, 2.0 parts of white sugar, 2.5 parts of gypsum, 0.8 part of monopotassium phosphate and 0.2 part of magnesium sulfate. In this embodiment, the weight ratio of the birch wood chips is determined, and the weight parts of the beet pulp are adjusted, so as to adjust the weight ratio of the beet pulp: the minimum value of the birch sawdust under the fixed value is experimentally researched, if the weight part of the beet pulp is continuously reduced, a grayish brown residue is caused after the culture medium is used at a later stage, and the main components of the residue are birch sawdust and wheat bran powder through testing, so that the formula is not scientific.
Furthermore, water is added into the culture medium on the basis of raw materials, and the water content of the culture medium is controlled to be 65-70%.
The production method of Dictyophora rubrovalvata glass bottle mother strain by using any one of the culture media comprises the following steps:
step 1) preparing a culture medium material required for producing Dictyophora rubrovalvata mother strains according to the mixture ratio requirement;
step 2) calculating the use amount of each raw material component of the production mother culture medium, diluting the soluble substances by using a small basin, and pouring the diluted solution into a container filled with 80% of the calculated total water amount for secondary dilution;
uniformly scattering insoluble substances into a stirrer, performing dry stirring for 20 minutes to obtain a dry stirring material, finally adding the diluted solution into the dry stirring material, and stirring for 30 minutes, wherein water is continuously added during stirring, and the water content of the culture medium is controlled to be 65-70%; obtaining a culture material;
step 3) filling the compost into a glass bottle according to the standard of loosening and tightening, filling sawdust to two centimeters away from the bottle opening, pressing the surface to be flat, filling a punching rod for punching, cleaning the glass bottle opening, sealing the glass bottle opening, and then boxing and sterilizing;
the sterilization requirements are as follows: (1) the temperature of the sterilizer must reach more than 100 ℃ within 1 hour; (2) the high pressure lasts for 3 hours at 121 ℃, and the normal pressure lasts for 11 hours above 100 ℃; (3) after sterilization, naturally reducing the temperature in the sterilizer to 60 ℃, slowly opening a door of the sterilizer to 20-30 cm (preventing external cold air from adsorbing bacteria sticks to form water drops), carrying the original seed bottles to a cooling chamber after waiting for one hour, and carrying out next inoculation after cooling to 20 +/-2 ℃;
step 4) sterilizing the inoculation tool, wherein the whole inoculation environment is in an aseptic environment;
step 5) cutting the selected robust dictyophora rubrovolvata eggs by using an inoculating knife, and taking two stipes (with high cell activity) parts in the dictyophora rubrovolvata eggs as inoculating blocks; inserting the inoculation block into a glass bottle for inoculation; the size of the inoculation block is controlled to be 1.5 centimeters long and wide (the size of a thumb nail cover), after inoculation, the rubber plug is wiped by alcohol, and then the opening is sealed;
and 6) uniformly placing the inoculated mother seeds in a culture chamber for mother seed culture.
Further, the soluble substances are white sugar, monopotassium phosphate and magnesium sulfate.
Further, the insoluble substances are birch sawdust, wheat bran powder, soybean meal, beet pulp and gypsum.
Further, the inoculation method of inserting the inoculation block into the glass bottle comprises the steps of obliquely inserting the inoculation block into the culture material from the bottle mouth at the position 1 cm and 3 cm below the culture material, and inoculating the inoculation block close to the bottle surface (the inoculation is convenient for observing the later growth condition at the bottle surface, and the two-point inoculation method can be adopted to enable the original seeds to grow rapidly, so that the growth period is shortened).
Two-point inoculation method: the two-point inoculation method is characterized in that the inoculation is carried out at the positions 1 cm and 3 cm below the culture material and close to the bottle surface.
TABLE 1 comparison of the present invention with the background art prior art (rough average)
The above description is only a part of specific embodiments of the present invention (since the formula of the present invention includes numerical ranges, the embodiments are not exhaustive, the protection scope of the present invention includes the numerical ranges and other technical point ranges), and the detailed contents or common knowledge known in the schemes are not described too much herein. It should be noted that the above-mentioned embodiments do not limit the present invention in any way, and all technical solutions obtained by means of equivalent substitution or equivalent transformation for those skilled in the art are within the protection scope of the present invention. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. The culture medium for producing the dictyophora rubrovolvata mother strain is characterized in that raw materials of the culture medium consist of birch sawdust, wheat bran powder, soybean meal, beet pulp, white sugar, gypsum, monopotassium phosphate and magnesium sulfate.
2. The culture medium for producing dictyophora rubrovolvata mother seeds according to claim 1, wherein 70-80 parts of birch sawdust, 10-20 parts of wheat bran powder, 2-8 parts of soybean powder, 3-6 parts of beet pulp, 0.5-2.0 parts of white sugar, 0.5-2.5 parts of gypsum, 0.2-0.8 part of monopotassium phosphate and 0.2-1.0 part of magnesium sulfate by weight are taken as raw materials of the culture medium.
3. The culture medium for producing dictyophora rubrovolvata mother seeds according to claim 1, wherein in raw materials of the culture medium, by weight, 74 parts of birch sawdust, 15 parts of wheat bran powder, 4 parts of soybean meal, 4 parts of beet pulp, 1.5 parts of white sugar, 1 part of gypsum, 0.3 part of potassium dihydrogen phosphate and 0.2 part of magnesium sulfate are contained.
4. The culture medium for producing dictyophora rubrovolvata mother seeds as claimed in claim 1, wherein raw materials of the culture medium comprise, by weight, 80 parts of birch sawdust, 10 parts of wheat bran powder, 2 parts of soybean powder, 6 parts of beet pulp, 0.5 part of white sugar, 0.5 part of gypsum, 0.2 part of potassium dihydrogen phosphate and 1.0 part of magnesium sulfate.
5. The culture medium for producing dictyophora rubrovolvata mother seeds according to claim 1, wherein in raw materials of the culture medium, by weight, 70 parts of birch sawdust, 20 parts of wheat bran powder, 8 parts of soybean meal, 3 parts of beet pulp, 2.0 parts of white sugar, 2.5 parts of gypsum, 0.8 part of monopotassium phosphate and 0.2 part of magnesium sulfate.
6. The culture medium for producing dictyophora rubrovolvata mother strains according to any one of claims 1 to 5, wherein water is added to the culture medium on the basis of raw materials, and the water content of the culture medium is controlled to be between 65 and 70 percent.
7. A production method of Dictyophora rubrovalvata glass bottle stock culture by using the culture medium of any one of claim 6, wherein the production method comprises the following steps:
step 1) preparing a culture medium material required for producing Dictyophora rubrovalvata mother strains according to the mixture ratio requirement;
step 2) calculating the use amount of each raw material component of the produced mother culture medium, diluting the soluble substances by using a small basin, and pouring the diluted solution into a container filled with 80 percent of the calculated total water amount for secondary dilution;
uniformly scattering insoluble substances into a stirrer, performing dry stirring for 20 minutes to obtain a dry stirring material, finally adding the diluted solution into the dry stirring material, and stirring for 30 minutes, wherein water is continuously added during stirring, and the water content of the culture medium is controlled to be 65-70%; obtaining a culture material;
step 3) filling the compost into a glass bottle according to the standard of loosening and tightening, filling sawdust to two centimeters away from the bottle opening, pressing the surface to be flat, filling a punching rod for punching, cleaning the glass bottle opening, sealing the glass bottle opening, and then boxing and sterilizing;
the sterilization requirements are as follows: (1) the temperature of the sterilizer must reach more than 100 ℃ within 1 hour; (2) the high pressure lasts for 3 hours at 121 ℃, and the normal pressure lasts for 11 hours above 100 ℃; (3) naturally cooling the temperature in the sterilizer to 60 ℃ after sterilization, slowly opening a sterilizer door to 20-30 cm, carrying the original seed bottle to a cooling chamber after waiting for one hour, and carrying out next inoculation after cooling to 20 +/-2 ℃;
step 4) sterilizing the inoculation tool, wherein the whole inoculation environment is in an aseptic environment;
step 5) cutting the selected robust dictyophora rubrovolvata eggs by using an inoculating knife, and taking two stipe parts in the dictyophora rubrovolvata eggs as inoculating blocks; inserting the inoculation block into a glass bottle for inoculation;
the size of the inoculation block is controlled to be 1.5 centimeters long and wide, and after inoculation, the rubber plug is wiped by alcohol and then sealed;
and 6) uniformly placing the inoculated mother seeds in a culture chamber for mother seed culture.
8. The method for producing Dictyophora rubrovalvata glass bottle stock culture according to claim 1, wherein the soluble substances are white sugar, potassium dihydrogen phosphate and magnesium sulfate.
9. The method for producing Dictyophora rubrovalvata glass bottle stock culture according to claim 1, wherein the insoluble substance is birch wood chips, wheat bran powder, soybean powder, beet pulp, gypsum.
10. The method for producing Dictyophora rubrovalvata glass bottle mother strain according to claim 1, wherein the inoculation block is inserted into the glass bottle for inoculation, and is obliquely inserted into the culture medium from the bottle mouth at 1 cm and 3 cm downward, and is inoculated at the position close to the bottle surface.
Priority Applications (1)
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