CN115093942A - Detection equipment and method for simultaneously detecting multiple chrysanthemum common viruses - Google Patents

Detection equipment and method for simultaneously detecting multiple chrysanthemum common viruses Download PDF

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CN115093942A
CN115093942A CN202210817677.9A CN202210817677A CN115093942A CN 115093942 A CN115093942 A CN 115093942A CN 202210817677 A CN202210817677 A CN 202210817677A CN 115093942 A CN115093942 A CN 115093942A
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centrifugal
tube
groove
chrysanthemum
guide cylinder
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姜自红
孙钦花
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Chuzhou Vocational and Technical College
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Chuzhou Vocational and Technical College
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B11/00—Feeding, charging, or discharging bowls
    • B04B11/04—Periodical feeding or discharging; Control arrangements therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention relates to the technical field of biology, in particular to a detection device and a detection method for simultaneously detecting multiple chrysanthemum common viruses, which comprise a centrifugal tube; the guide cylinder is annularly provided with a plurality of conducting assemblies, and the conducting assemblies penetrate through the guide cylinder downwards and are used for conducting the centrifugal tube downwards and upwards; the rotating piece is arranged above the guide cylinder, a plurality of centrifugal grooves are annularly distributed on the bottom surface of the rotating piece, the centrifugal grooves are aligned with the conduction assembly, and stoppers are arranged in the centrifugal grooves and used for vertically limiting the centrifugal tubes in the centrifugal grooves; according to the automatic centrifugal extraction device, the centrifuge tube in the centrifugal groove is automatically replaced by controlling the lifting and twisting of the turntable and matching with the conduction of the conduction assembly to the centrifuge tube, the corresponding reagents are injected into the centrifuge tube by adopting a plurality of groups of injectors, and the absorbers are used for controlling the centrifuge tube to generate an adsorption effect, so that the centrifugal processing of the centrifuge tube and repeated supernatant extraction are realized, the automatic centrifugal extraction of chrysanthemum RNA is realized, the efficiency is high, and misoperation is avoided.

Description

Detection equipment and method for simultaneously detecting multiple chrysanthemum common viruses
Technical Field
The invention relates to the technical field of biology, in particular to a detection device and a detection method for simultaneously detecting multiple chrysanthemum common viruses.
Background
The chrysanthemum has higher medicinal value, the market is great to the demand of chrysanthemum, the cuttage is the main mode that current chrysanthemum was planted, but this mode spreads the virus easily, and the virus has the multiple, has greatly reduced the quality of chrysanthemum, does not have special effect medicine moreover and can reverse multiple virus in the chrysanthemum, consequently carries out virus detection at the cuttage reproduction to the chrysanthemum to it is comparatively ideal mode to screen out sick strain.
The existing chrysanthemum virus detection methods are various, an RT-PCR detection system is a relatively accurate plant virus detection method, RT-PCR is a detection technology combining reverse transcription of RNA virus and PCR amplification, and can simultaneously detect various viruses in chrysanthemum.
Adopt RT-PCR detecting system to need to extract the RNA composition of chrysanthemum plant to chrysanthemum virus detection, traditional chrysanthemum RNA extraction mode is ground the chrysanthemum plant into powder in liquid nitrogen, add multiple reagent in proper order with the powder again and centrifuge, extract the supernatant, centrifuge again and extract a plurality of flows such as supernatant, finally obtain the RNA composition of chrysanthemum, this process needs manual operation, need frequently change the container and get liquid subassembly for avoiding the reagent doping of a plurality of flows in addition, make whole process consuming time longer.
Disclosure of Invention
The invention aims to solve the following problems in the prior art: traditional chrysanthemum RNA extraction mode is ground the chrysanthemum plant into powder in the liquid nitrogen, adds multiple reagent in proper order with the powder again and centrifuges, draws a supernatant, again a plurality of flows such as centrifugation extraction supernatant, finally obtains the RNA composition of chrysanthemum, and this process needs manual operation, need frequently change the container and get the liquid subassembly for the reagent doping of avoiding a plurality of flows in addition, makes whole process consuming time longer.
In order to solve the problems in the prior art, the invention provides a detection device and a detection method for simultaneously detecting multiple chrysanthemum common viruses, which comprises a centrifugal tube;
the guide cylinder is annularly distributed with a plurality of conduction assemblies, and the conduction assemblies penetrate through the guide cylinder downwards and are used for conducting the centrifugal tube downwards and upwards;
the rotating piece is arranged above the guide cylinder, a plurality of centrifugal grooves are annularly distributed on the bottom surface of the rotating piece, the centrifugal grooves are aligned with the conduction assembly, and stoppers are arranged in the centrifugal grooves and used for vertically limiting the centrifugal tubes in the centrifugal grooves;
electric putter, electric putter arrange in the rotating member top, electric putter pass through the sealed shell with the guide cylinder is fixed, and electric putter's flexible end is connected perpendicularly the rotating member, the upper and lower removal of electric putter drive rotating member for the reagent groove with get between the conduction subassembly and put the centrifuging tube.
Preferably, the sealing shell is fixedly sleeved at the top of the material guide cylinder, the rotating part is rotatably matched inside the sealing shell, the electric push rod is installed at the top of the sealing shell, the telescopic end of the electric push rod is rotatably connected with the center of the top of the rotating part, the motor A is installed on the outer wall of the sealing shell, the outer wall of the rotating part is fixedly provided with a toothed ring, and the shaft end of the motor A is fixedly provided with a gear which is in meshing transmission with the toothed ring.
Preferably, the rotating member includes the carousel, the centrifugal slot annular distributes in the carousel bottom surface, the stopper includes flexible electro-magnet, and flexible electro-magnet imbeds at the centrifugal slot side, and the end fixing of flexible electro-magnet has the stopper that the level inserted the centrifugal slot, centrifuging tube and centrifugal slot clearance adaptation, centrifuging tube fixed surface have the ear piece of counterpointing the stopper.
Preferably, a plurality of reagent tanks are arranged in the rotary table, each reagent tank is connected with one liquid injector, a liquid injection pipe penetrates through the top of the centrifugal tank, each liquid injector is communicated with each liquid injection pipe through a serial pipe, an adsorber is arranged in the rotary table, and the adsorber is communicated with the liquid injection pipes through the serial pipes.
Preferably, the injection tube comprises a piston cylinder, two sets of check valves with opposite guide directions are arranged at the end part of the piston cylinder, the two sets of check valves are respectively connected with the serial pipe and the reagent tank and used for conducting the serial pipe in a one-way manner in the reagent tank, the piston cylinder slides elastically through a spring to form a piston handle, an electromagnet block is fixed at the end part of the piston handle, a permanent magnet is fixed on the outer wall of the piston cylinder, the adsorber and the injection tube have the same structure, the conducting directions of the two check valves of the adsorber are opposite to the conducting directions of the two check valves of the injection tube, and the two sets of check valves of the adsorber are used for conducting the external space in a one-way manner in the injection tube.
Preferably, the conduction subassembly include with the last silo of centrifuge tube clearance adaptation and arrange the silo, go up silo and row silo and run through perpendicularly the guide cylinder, the side of going up silo and row silo has the extension groove of adaptation ear piece, installs driving belt in the extension inslot, and driving belt's area body surface equidistance is fixed with the plectrum that is used for bearing the ear piece, and driving belt's axle head is fixed with bevel gear, the top of guide cylinder is rotated there is the drive ring, and the drive ring outside has the oblique flank of tooth of being connected with bevel gear meshing, and the guide cylinder top is provided with motor B, and motor B's axle head is fixed with the gear, the inboard of drive ring has the meshing the tooth face of gear.
Preferably, the centrifuging tube includes the tube, and the bottom of tube is for leaking hopper-shaped, and the inboard adaptation of the flare opening of tube has the awl piece, and tube inside is fixed with the needle handle, and the awl piece slides with the bottom of needle handle and pegs graft, and the awl piece passes through spring elastic connection needle handle, and the tube mouth has at the tube shell top, the mouth of pipe and the adaptation of annotating the agent pipe.
Preferably, the top of the tube shell is in threaded connection with a tube cover, the tube cover is provided with a funnel surface, the tube opening is arranged at the bottom of the funnel surface of the tube cover, and the funnel surface of the tube cover is uniformly provided with crack grooves.
A detection method for multiple chrysanthemum common viruses based on the detection equipment comprises the following specific steps:
A. placing the centrifuge tube for placing the chrysanthemum sample powder in a centrifuge tank, limiting and fixing by a limiter, placing a plurality of detection reagents in a reagent tank, and arranging clean centrifuge tubes in a feeding tank;
B. opening a corresponding liquid injector to inject a first reagent into the centrifugal tube, and driving a turntable to rotate through a motor A to centrifugally process sample liquid in the centrifugal tube;
C. the electric push rod is started to drive the rotary table to move downwards to remove the limit of the limit device, so that the centrifugal tube after the first centrifugal treatment is placed at the top of the discharge groove, the rotary table is twisted, the clean centrifugal tube in the feeding groove is placed in the centrifugal groove and limited by the limit device, and the rotary table is rotated to align a new centrifugal tube to the centrifugal tube after the first centrifugal treatment;
D. starting an electric push rod to drive the rotary table to move downwards, inserting a new centrifugal tube into the centrifugal tube after the first centrifugation, adsorbing gas in the centrifugal tube through the adsorber, and adsorbing supernatant in the centrifugal tube after the centrifugation;
E. opening a corresponding injector to inject a second reagent into the centrifugal tube, driving the turntable to rotate through the motor A, carrying out second centrifugal treatment on the sample liquid in the centrifugal tube, sequentially repeating the steps A-D, completing multiple centrifugal treatment on chrysanthemum tissue, and finally obtaining an RNA substance of chrysanthemum;
F. the centrifuge tube after being used is discharged downwards from the discharge groove through the conduction assembly, and a new centrifuge tube is conducted upwards from the feeding groove and is used for centrifuging and replacing the centrifuge tube at each time.
Compared with the related technology, the detection equipment and the method for simultaneously detecting the multiple chrysanthemum common viruses have the following beneficial effects:
1. according to the invention, through the lifting and torsion control of the turntable and the conduction of the conduction assembly to the centrifugal tube, the replacement of the centrifugal tube in the centrifugal groove is automatically realized, a plurality of groups of liquid injectors are adopted to inject corresponding reagents into the centrifugal tube, and the adsorber is used for controlling the centrifugal tube to generate adsorption, so that the centrifugal processing of the centrifugal tube and repeated supernatant extraction are realized automatically, the automatic centrifugal extraction of chrysanthemum RNA is realized, the efficiency is high, and the misoperation is avoided;
2. the top of the centrifugal tube is provided with the tearing type top opening, the bottom of the centrifugal tube is limited by the elastically moving conical block and is used for automatically sealing the centrifugal tube and automatically opening the centrifugal tube when supernatant is extracted, and the supernatant extraction action is simplified.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of a rotating member mounting structure of the present invention;
FIG. 3 is a schematic view of the internal structure of the turntable according to the present invention;
FIG. 4 is a schematic view of the stopper of the present invention;
FIG. 5 is a schematic view of a centrifuge tube according to the present invention;
FIG. 6 is a schematic cross-sectional view of a centrifuge tube of the present invention;
FIG. 7 is a schematic diagram of the alignment of the injection tube and the centrifuge tube according to the present invention;
FIG. 8 is a schematic view of the structure of the injector according to the present invention;
FIG. 9 is a schematic view of a conductive assembly of the present invention;
fig. 10 is a schematic diagram of the alignment of the pick and the ear piece of the present invention.
The reference numbers in the figures: 1. a material guide cylinder; 2. a turntable; 3. sealing the housing; 4. an electric push rod; 5. a motor A; 6. a toothed ring; 7. centrifuging the tube; 71. a pipe shell; 72. a tube cover; 73. a pipe orifice; 74. a crack mark groove; 75. a needle handle; 76. a conical block; 8. a liquid injector; 81. a piston cylinder; 82. a one-way valve; 83. a permanent magnet; 84. an electromagnet block; 85. a piston handle; 9. an adsorber; 10. a pipe is connected in series; 11. a reagent tank; 12. an agent injection pipe; 13. an ear piece; 14. a centrifugal groove; 15. a telescopic electromagnet; 16. a limiting block; 17. a feeding trough; 18. a discharge chute; 19. a drive belt; 20. a bevel gear; 21. a drive ring; 22. a motor B; 23. a shifting sheet.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Specific implementations of the present invention are described in detail below with reference to specific embodiments.
Example one
As shown in FIGS. 9-10, a device for simultaneously detecting a plurality of chrysanthemum common viruses, a guide cylinder 1 is cylindrical, a plurality of conducting assemblies are annularly distributed around the surface of the guide cylinder 1, each conducting assembly comprises an upper material groove 17 and a discharge groove 18, the upper material groove 17 and the discharge groove 18 vertically penetrate through the guide cylinder 1, the upper material groove 17 and the discharge groove 18 are in clearance fit with a centrifuge tube 7, a through extending groove is formed in the inner sides of the feeding groove 17 and the discharging groove 18, a transmission belt 19 is installed in the extending groove, shifting pieces 23 are fixed on the surface of the belt body of the transmission belt 19 at equal intervals, a bevel gear 20 is fixed at the top shaft end of the transmission belt 19, a transmission ring 21 is rotated at the top of the guide cylinder 1, an inclined tooth surface meshed with the bevel gear 20 is arranged on the outer side of the transmission ring 21, a motor B22 is arranged at the top of the guide cylinder 1, a gear is fixed at the shaft end of a motor B22, and a tooth surface meshed with the gear is arranged on the inner side of the transmission ring 21;
the ear blocks 13 are fixed on the side edges of the centrifuge tubes 7, a plurality of clean centrifuge tubes 7 are vertically placed in the feeding groove 17, the ear blocks 13 are placed in the extension grooves, each poking piece 23 supports one ear block 13, the centrifuge tubes 7 are arranged at equal intervals, the centrifuge tubes 7 after being used are placed in the discharging groove 18, the centrifuge tubes 7 after being used are also supported by the corresponding poking pieces 23, the motor B22 is started to drive the transmission ring 21 to rotate, the transmission belt 19 is driven by the bevel gear 20 to circularly guide, the centrifuge tubes 7 in the feeding groove 17 are supported by the poking pieces 23 to move upwards for replacing new centrifuge tubes 7 by rotating pieces, the centrifuge tubes 7 after being used in the discharging groove 18 are supported by the poking pieces 23 to move downwards until being discharged, and convenient replacement of the centrifuge tubes 7 is realized;
as shown in fig. 1-4, the rotating member is arranged above the guide cylinder 1, the rotating member includes a rotating disk 2, a plurality of centrifugal slots 14 are annularly distributed on the bottom surface of the rotating disk 2, the centrifugal slots 14 are aligned with a feeding slot 17, the stopper includes a telescopic electromagnet 15, the telescopic electromagnet 15 is embedded in the side edge of the centrifugal slot 14, a stopper 16 horizontally inserted into the centrifugal slot 14 is fixed at the end of the telescopic electromagnet 15, the centrifugal tube 7 is in clearance fit with the centrifugal slot 14, and an ear piece 13 of the alignment stopper 16 is fixed on the surface of the centrifugal tube 7;
the sealing shell 3 is fixedly sleeved on the top of the guide cylinder 1, the rotary table 2 is rotatably matched in the sealing shell 3, the electric push rod 4 is installed on the top of the sealing shell 3, the telescopic end of the electric push rod 4 is rotatably connected with the central position of the top of the rotary table 2, the outer wall of the sealing shell 3 is provided with a motor A5, the outer wall of the rotary table 2 is fixedly provided with a toothed ring 6, and the shaft end of the motor A5 is fixedly provided with a gear which is in meshing transmission with the toothed ring 6;
the centrifugal tube 7 is inserted into the centrifugal groove 14, the limiting block 16 is clamped below the lug 13 through the extension of the telescopic electromagnet 15 to limit the centrifugal tube 7 in the centrifugal groove 14, the motor A5 drives the toothed ring 6 to drive the turntable 2 to rotate for carrying out centrifugal treatment on substances in the centrifugal tube 7, the motor A5 drives the turntable 2 to twist and change positions, the electric push rod 4 is matched with the turntable 2 to drive the turntable 2 to move up and down, and the centrifugal groove 14 is used for changing positions between the feeding groove 17 and the discharging groove 18 to pick and place the centrifugal tube 7;
as shown in fig. 3 and 8, a plurality of reagent tanks 11 are arranged in a rotary disc 2, each reagent tank 11 is connected with one injector 8, the top of a centrifugal tank 14 is penetrated with an injection pipe 12, each injector 8 is communicated with each injection pipe 12 through a serial pipe 10, an absorber 9 is arranged in the rotary disc 2, the absorber 9 is communicated with the injection pipe 12 through the serial pipe 10, the injection pipe 12 comprises a piston cylinder 81, the end part of the piston cylinder 81 is provided with two sets of one-way valves 82 with opposite directions, the two sets of one-way valves 82 are respectively connected with the serial pipe 10 and the reagent tank 11 and are used for one-way conduction of the serial pipe 10 of the reagent tanks 11, the piston cylinder 81 elastically slides through a spring to be provided with a piston handle 85, the end part of the piston handle 85 is fixed with an electromagnet block 84, the outer wall of the piston cylinder 81 is fixed with a permanent magnet 83, the structure of the absorber 9 is the same as that of the injection pipe 12, the conduction direction of the two one-way valves 82 of the absorber 9 is opposite to the conduction direction of the two one-way valves 82 of the injection pipe 12, two sets of check valves 82 of the absorber 9 are used for the one-way conduction of the agent injection pipe 12 to the external space;
when the centrifugal tube 7 is limited in the centrifugal groove 14, the injection tube 12 is communicated with the centrifugal tube 7, and the working programs of each liquid injector 8 and the adsorber 9 are set by the programs, so that the reagents are orderly and quantitatively added into the centrifugal tube 7;
under a normal state, the electromagnet block 84 is attached with the permanent magnet 83, so that the compression spring of the piston handle 85 is inserted into the piston cylinder 81, when a reagent needs to be added into the centrifugal tube 7, the electromagnet block 84 is powered off, the piston handle 85 is expanded under the action of elastic force, the reagent in the corresponding reagent groove 11 is sucked into the piston cylinder 81 through the one-way valve 82, the electromagnet block 84 is powered on again, the piston handle 85 is closed into the piston cylinder 81, the reagent is introduced into the serial tube 10 through the other one-way valve 82, and then the reagent is injected into the centrifugal tube 7 through the reagent injection tube 12;
similarly, the absorber 9 is opened to move in a telescopic manner, so that the gas in the centrifugal tube 7 is pumped out, and the gas is used for extracting the supernatant in the centrifugal tube 7 after centrifugation by a new centrifugal tube 7;
as shown in fig. 5-7, the centrifugal tube 7 includes a tube shell 71, the bottom of the tube shell 71 is funnel-shaped, the top of the tube shell 71 is connected with a tube cap 72 through a screw thread, the tube cap 72 has a funnel surface, a tube mouth 73 is arranged at the bottom of the funnel surface of the tube cap 72, a crack groove 74 is uniformly formed on the funnel surface of the tube cap 72, a conical block 76 is adapted to the inner side of the funnel mouth of the tube shell 71, a needle handle 75 is fixed inside the tube shell 71, the conical block 76 is inserted into the bottom end of the needle handle 75 in a sliding manner, and the conical block 76 is elastically connected with the needle handle 75 through a spring.
When the tube shell 71 is limited in the centrifugal groove 14, the injection tube 12 is in fit communication with the nozzle 73 for conducting the reagent to the inside of the tube shell 71 and extracting the air in the tube shell 71;
when a new centrifugal tube 7 extracts supernatant from the centrifuged centrifugal tube 7, the bottom of the funnel of the upper centrifugal tube 7 is inserted into the tube opening 73, and with continuous insertion, the tube cover 72 is torn along the crack groove 74, so that the bottom of the funnel of the upper centrifugal tube 7 is inserted into the lower tube shell 71, the conical block 76 on the upper part of the upper centrifugal tube is extruded by the top end of the lower needle handle 75 to overcome the elasticity and move upwards, a gap is formed between the conical block 76 and the tube shell 71, the supernatant in the lower tube shell 71 is absorbed into the upper tube shell 71 through the gap, then the upper centrifugal tube 7 moves upwards, the conical block 76 blocks the bottom opening of the tube shell 71 under the action of the elasticity, and the adsorber 9 needs to be continuously opened to work in the process;
the cap 72 is threadably connected to the cartridge 71 for subsequent reuse of the cartridge 71 to replace the torn cap 72.
A detection method for multiple chrysanthemum common viruses based on the detection equipment comprises the following specific steps:
A. the centrifuge tube 7 for placing the chrysanthemum sample powder is placed in the centrifuge groove 14 and limited and fixed by a limiter, a plurality of detection reagents are placed in the reagent groove 11, and the clean centrifuge tube 7 is arranged in the feeding groove 17;
B. opening a corresponding liquid injector 8 to inject a first reagent into the centrifuge tube 7, and driving the turntable 2 to rotate through a motor A5 to centrifugally process sample liquid in the centrifuge tube 7;
C. starting the electric push rod 4 to drive the turntable 2 to move downwards to remove the limit of the limit device, placing the centrifugal tube 7 subjected to the first centrifugation treatment on the top of the discharge groove 18, twisting the turntable 2 to place the clean centrifugal tube 7 in the feed groove 17 into the centrifugal groove 14 to be limited by the limit device, and rotating the turntable 2 to align a new centrifugal tube 7 with the centrifugal tube 7 subjected to the first centrifugation;
D. starting the electric push rod 4 to drive the turntable 2 to move downwards, so that a new centrifugal tube 7 is inserted into the centrifugal tube 7 after the first centrifugation, and the adsorber 9 adsorbs gas in the centrifugal tube 7, so that the new centrifugal tube 7 adsorbs supernatant in the centrifugal tube 7 after the centrifugation;
E. opening a corresponding liquid injector 8 to inject a second reagent into the centrifuge tube 7, driving the turntable 2 to rotate through the motor A5, carrying out secondary centrifugal treatment on the sample liquid in the centrifuge tube 7, repeating the steps A-D in sequence, completing multiple centrifugal treatment on chrysanthemum tissues, and finally obtaining the RNA substance of chrysanthemum;
F. the used centrifuge tube 7 is discharged downwardly from the discharge chute 18 by the conducting assembly and a new centrifuge tube 7 is conducted upwardly from the loading chute 17 for each centrifuge change of centrifuge tube 7.

Claims (9)

1. A detection device for simultaneously detecting multiple chrysanthemum common viruses is characterized by comprising:
a centrifuge tube (7);
the guide cylinder (1), a plurality of conduction assemblies are annularly distributed on the guide cylinder (1), and the conduction assemblies penetrate through the guide cylinder (1) downwards and are used for conducting the centrifugal tube (7) downwards and upwards;
the rotating piece is arranged above the guide cylinder (1), a plurality of centrifugal grooves (14) are annularly distributed on the bottom surface of the rotating piece, the centrifugal grooves (14) are aligned with the conduction assembly, and stoppers are arranged in the centrifugal grooves (14) and used for vertically limiting the centrifugal tube (7) in the centrifugal grooves (14);
electric putter (4), electric putter (4) are arranged in the rotating member top, electric putter (4) through sealed shell (3) with guide cylinder (1) is fixed, and the flexible end of electric putter (4) is connected perpendicularly the rotating member, electric putter (4) drive rotating member move up and down for reagent groove (11) with get between the conduction subassembly and put centrifuging tube (7).
2. The detection device for simultaneously detecting multiple chrysanthemum common viruses according to claim 1, wherein the sealing shell (3) is fixedly sleeved on the top of the material guide cylinder (1), the rotating part is rotatably matched inside the sealing shell (3), the electric push rod (4) is installed on the top of the sealing shell (3), the telescopic end of the electric push rod (4) is rotatably connected with the center of the top of the rotating part, the outer wall of the sealing shell (3) is provided with a motor A (5), the outer wall of the rotating part is fixedly provided with a toothed ring (6), and the shaft end of the motor A (5) is fixedly provided with a gear which is in meshing transmission with the toothed ring (6).
3. The detection apparatus for detect common virus of multiple chrysanthemum simultaneously according to claim 2, characterized in that, the rotating member includes carousel (2), centrifugal groove (14) annular distribution is in carousel (2) bottom surface, the stopper includes flexible electro-magnet (15), and flexible electro-magnet (15) embedding is at centrifugal groove (14) side, and the end fixing of flexible electro-magnet (15) has stopper (16) that the level inserted in centrifugal groove (14), centrifuging tube (7) and centrifugal groove (14) clearance adaptation, centrifuging tube (7) fixed surface has ear piece (13) of counterpointing stopper (16).
4. The device for detecting multiple common chrysanthemum viruses simultaneously according to claim 3, wherein the rotary disc (2) is internally provided with a plurality of reagent tanks (11), each reagent tank (11) is connected with one liquid injector (8), the top of the centrifugal tank (14) is provided with a liquid injection pipe (12) in a penetrating way, each liquid injector (8) is communicated with each liquid injection pipe (12) through a serial pipe (10), an adsorber (9) is arranged in the rotary disc (2), and the adsorber (9) is communicated with the liquid injection pipes (12) through the serial pipes (10).
5. The apparatus for detecting multiple chrysanthemum common viruses according to claim 4, the injection pipe (12) comprises a piston cylinder (81), two sets of one-way valves (82) with opposite directions are arranged at the end part of the piston cylinder (81), the two sets of one-way valves (82) are respectively connected with the serial pipe (10) and the reagent tank (11), is used for the reagent tank (11) to be communicated with the serial pipe (10) in a one-way, the piston cylinder (81) is elastically slid by a spring to be provided with a piston handle (85), the end part of the piston handle (85) is fixed with an electromagnet block (84), the outer wall of the piston cylinder (81) is fixed with a permanent magnet (83), the structure of the adsorber (9) is the same as that of the injection pipe (12), the conduction direction of the two one-way valves (82) of the adsorber (9) is opposite to that of the two one-way valves (82) of the injection pipe (12), and the two groups of one-way valves (82) of the adsorber (9) are used for conducting the external space in one way through the injection pipe (12).
6. The device for simultaneously detecting multiple common chrysanthemum viruses according to claim 3, wherein the conducting assembly comprises a feeding groove (17) and a discharging groove (18) which are in clearance fit with the centrifuge tube (7), the feeding groove (17) and the discharging groove (18) vertically penetrate through the guide cylinder (1), the side edges of the feeding groove (17) and the discharging groove (18) are provided with extending grooves of the fitting lug blocks (13), a driving belt (19) is installed in the extending grooves, the surface of the belt body of the driving belt (19) is equidistantly fixed with shifting pieces (23) for bearing the lug blocks (13), the shaft end of the driving belt (19) is fixed with a bevel gear (20), the top of the guide cylinder (1) is rotated with a driving ring (21), the outer side of the driving ring (21) is provided with an inclined tooth surface meshed and connected with the bevel gear (20), the top of the guide cylinder (1) is provided with a motor B (22), a gear is fixed at the shaft end of the motor B (22), and the inner side of the transmission ring (21) is provided with a gear surface meshed with the gear.
7. The device for simultaneously detecting the multiple chrysanthemum common viruses according to claim 4, wherein the centrifuge tube (7) comprises a tube shell (71), the bottom of the tube shell (71) is funnel-shaped, a conical block (76) is adapted to the inner side of a funnel opening of the tube shell (71), a needle handle (75) is fixed inside the tube shell (71), the conical block (76) is in sliding insertion with the bottom end of the needle handle (75), the conical block (76) is elastically connected with the needle handle (75) through a spring, a tube opening (73) is formed in the top of the tube shell (71), and the tube opening (73) is adapted to the injection tube (12).
8. The device for detecting multiple chrysanthemum common viruses simultaneously according to claim 1, wherein a tube cover (72) is in threaded connection with the top of the tube shell (71), the tube cover (72) is provided with a funnel surface, the tube opening (73) is arranged at the bottom of the funnel surface of the tube cover (72), and the funnel surface of the tube cover (72) is uniformly provided with crack grooves (74).
9. A method for detecting multiple chrysanthemum common viruses based on the detection equipment of any one of claims 1 to 8 is characterized by comprising the following specific steps:
A. the centrifugal tube (7) for placing the chrysanthemum sample powder is placed in the centrifugal groove (14) and limited and fixed by the limiting stopper, a plurality of detection reagents are placed in the reagent groove (11), and the clean centrifugal tube (7) is arranged in the material loading groove (17);
B. opening a corresponding liquid injector (8) to inject a first reagent into the centrifugal tube (7), and driving the turntable (2) to rotate through the motor A (5) to carry out centrifugal treatment on the sample liquid in the centrifugal tube (7);
C. an electric push rod (4) is started to drive a rotating disc (2) to move downwards to release the limit of a limit stop, so that the centrifugal tube (7) after the first centrifugal treatment is placed at the top of a discharge groove (18), the rotating disc (2) is twisted, the clean centrifugal tube (7) in a feeding groove (17) is placed in a centrifugal groove (14) and limited by the limit stop, and the rotating disc (2) is rotated to enable a new centrifugal tube (7) to align the centrifugal tube (7) after the first centrifugal treatment;
D. starting an electric push rod (4) to drive the turntable (2) to move downwards, so that a new centrifugal tube (7) is inserted into the centrifugal tube (7) after the first centrifugation, and the gas in the centrifugal tube (7) is adsorbed through the adsorber (9), so that the new centrifugal tube (7) adsorbs the supernatant in the centrifugal tube (7) after the centrifugation;
E. opening a corresponding liquid injector (8) to inject a second reagent into the centrifuge tube (7), driving the turntable (2) to rotate through the motor A (5), carrying out second centrifugal treatment on the sample liquid in the centrifuge tube (7), sequentially repeating the steps A-D, completing multiple centrifugal treatment on chrysanthemum tissue, and finally obtaining an RNA substance of chrysanthemum;
F. the used centrifuge tube (7) is discharged downwards from the discharge groove (18) through the conducting assembly, and a new centrifuge tube (7) is conducted upwards from the feeding groove (17) for replacing the centrifuge tube (7) at each centrifugation.
CN202210817677.9A 2022-07-12 2022-07-12 Detection equipment and method for simultaneously detecting multiple chrysanthemum common viruses Withdrawn CN115093942A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117619573A (en) * 2024-01-25 2024-03-01 科泰(山东)健康营养有限公司 Centrifugal equipment for galactomannan experiments
CN117732611A (en) * 2024-02-21 2024-03-22 辽宁百隆生物工程有限公司 Heparin sodium crude product extraction device and extraction method

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117619573A (en) * 2024-01-25 2024-03-01 科泰(山东)健康营养有限公司 Centrifugal equipment for galactomannan experiments
CN117619573B (en) * 2024-01-25 2024-03-26 科泰(山东)健康营养有限公司 Centrifugal equipment for galactomannan experiments
CN117732611A (en) * 2024-02-21 2024-03-22 辽宁百隆生物工程有限公司 Heparin sodium crude product extraction device and extraction method
CN117732611B (en) * 2024-02-21 2024-04-26 辽宁百隆生物工程有限公司 Heparin sodium crude product extraction device and extraction method

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Application publication date: 20220923