CN117619573A - Centrifugal equipment for galactomannan experiments - Google Patents

Abstract

The invention relates to the technical field of galactomannan experimental equipment, and discloses centrifugal equipment for galactomannan experiments, which comprises a centrifuge shell, a centrifuge upper cover arranged at the top of the centrifuge shell, a centrifugal rotor for centrifuging materials, a centrifugal test tube inserted on the centrifugal rotor, and a centrifugal motor for driving the centrifugal rotor to rotate, wherein a liquid taking lifting piece and a liquid taking air pump are arranged on the centrifuge upper cover, a liquid taking plate is connected at the lifting end of the liquid taking lifting piece, and a liquid taking mechanism for sucking supernatant in the centrifugal test tube is arranged on the liquid taking plate; the liquid taking mechanism comprises a liquid taking cylinder which is clamped with the liquid taking plate, a piston block which is arranged inside the liquid taking cylinder in a sliding manner, and a through hole which is formed in the bottom of the liquid taking cylinder, wherein the outer side of the through hole is detachably provided with a bevel needle, and when the operation of removing the supernatant can be automatically completed, the device replaces manual operation, so that the experimental manual steps are reduced, the experimental success rate is improved, and the difficulty and the pressure of the experimental operation are reduced.

Description

Centrifugal equipment for galactomannan experiments

Technical Field

The invention relates to the technical field of galactomannan experimental equipment, in particular to centrifugal equipment for galactomannan experiments.

Background

Galactomannans, or galactomannans, are polysaccharides that contain a mannose backbone with galactoside groups. Gastric cancer is a common tumor at present, and has the characteristics of high morbidity and high malignancy, and the mortality rate is the front of the related mortality rate of the tumor. New antitumor drugs are layered endlessly, but the long-term survival rate of gastric cancer patients is not obviously improved. Thus, there is an urgent need to find new methods and medicaments for inhibiting tumor proliferation. Galactomannans are widely used in foods and in clinic, and galactomannans extracted from beans are often used as stabilizers and viscosity enhancers in foods. However, the role of galactomannans in inhibiting gastric cancer cell proliferation has not been reported.

Human gastric cancer cells are adherent growth cells, and the culture method is the same as that of common adherent cells. After the cell growth medium has been digested, it is transferred to a centrifuge tube for centrifugation, and supernatant and precipitate are separated after centrifugation, since galactomannan is a polysaccharide, its solubility and viscosity may be affected by other substances in solution. If the supernatant contains substances that may affect the solubility and viscosity of the galactomannan, it may have an effect on the properties of the galactomannan. In order to ensure the accuracy of the experimental results, the supernatant after centrifugation needs to be removed before the galactomannan is added, only the precipitate is reserved, then the cells are inoculated, a dosing culture medium containing quantitative galactomannan is prepared by using the mother liquor, the dosing culture medium is added into an orifice plate, and a blank control group, namely a cell growth culture medium, is simultaneously arranged, and the cells are incubated in an incubator.

At present, the supernatant is usually removed manually by using a suction tube with a wing, and if a plurality of groups of control groups exist in the experiment, the step can take a long time, so that the progress and the result of the experiment are affected; therefore, the existing requirements are not met, and for this reason we propose a centrifugal device for galactomannan experiments.

Disclosure of Invention

The invention provides a centrifugal device for galactomannan experiments, which is provided with the beneficial effects that supernatant is automatically sucked and separated after centrifugation, manual operation is replaced, and experimental efficiency is improved, and the problems that in the background art, the supernatant is usually removed manually by using a suction tube with careful wings, and if a plurality of groups of control groups exist in the experiments, the step takes a long time, so that the progress and the result of the experiments are influenced are solved.

The invention provides the following technical scheme: the centrifugal equipment for the galactomannan experiments comprises a centrifugal machine shell, a centrifugal upper cover arranged at the top of the centrifugal machine shell, a centrifugal rotating head for centrifuging materials, a centrifugal test tube inserted on the centrifugal rotating head and a centrifugal motor for driving the centrifugal rotating head to rotate, wherein a liquid taking lifting piece and a liquid taking air pump are arranged on the centrifugal machine upper cover, the lifting end of the liquid taking lifting piece is connected with a liquid taking plate, and a liquid taking mechanism for sucking supernatant in the centrifugal test tube is arranged on the liquid taking plate;

the liquid taking mechanism comprises a liquid taking cylinder, a piston block and a through hole, wherein the liquid taking cylinder is clamped with the liquid taking plate, the piston block is arranged inside the liquid taking cylinder in a sliding mode, the through hole is formed in the bottom of the liquid taking cylinder, and a bevel needle is detachably arranged outside the through hole.

As an alternative to the centrifugal apparatus for galactomannan experiments described in the present invention, wherein: the utility model discloses a liquid taking device, including a liquid taking cylinder, a liquid taking plate, a piston block, a liquid taking pump, a sealing upper cover, a liquid taking cylinder top, a liquid taking plate and a plurality of sliding grooves, wherein the sealing upper cover is installed at the liquid taking cylinder top, the sealing upper cover is arranged, the liquid taking cylinder is arranged inside the liquid taking cylinder, a sealing space is formed above the piston block, the sealing upper cover is connected with a liquid taking air pipe, the other end of the liquid taking air pipe is connected with the air suction end of the liquid taking air pump, the piston block is in sliding fit with the inner wall of the liquid taking cylinder, and a plurality of sliding grooves are arranged on the liquid taking plate in an annular array manner.

As an alternative to the centrifugal apparatus for galactomannan experiments described in the present invention, wherein: the centrifugal machine upper cover bottom side fixed mounting has a plurality of spacing posts, and is a plurality of spacing post is vertical setting, spacing post runs through get the liquid board, get the liquid board with spacing post sliding fit.

As an alternative to the centrifugal apparatus for galactomannan experiments described in the present invention, wherein: get the liquid board and set up to the circular plate, centrifuge upper cover is inside to be provided with toothed sleeve, toothed sleeve sets up to the annular sleeve of inner wall distribution rack, toothed sleeve with get the coaxial setting of liquid board, it has offered a plurality of grooves of wearing to get liquid board edge annular array, wear to be provided with in the inslot and be used for driving get the tilting mechanism of liquid mechanism lift translation simultaneously.

As an alternative to the centrifugal apparatus for galactomannan experiments described in the present invention, wherein: the tilting mechanism is including rotating first gear and the second gear of installing in wearing the inslot, first gear with toothed sleeve's rack engagement, the second gear with first gear engagement, it is adjacent to get the liquid board wear groove department and install the sliding seat, slidable mounting has horizontal propulsion rack on the sliding seat, the second gear with horizontal propulsion rack engagement, horizontal propulsion rack's tip install with get liquid mechanism fixed connection's outsourcing ring.

As an alternative to the centrifugal apparatus for galactomannan experiments described in the present invention, wherein: the inner wall of the toothed sleeve is provided with a toothless part, the toothless part is a smooth inner wall without a rack, and when the liquid taking plate corresponds to the toothless part, the first gear is in clearance fit with the toothless part.

As an alternative to the centrifugal apparatus for galactomannan experiments described in the present invention, wherein: the tube mouth of the centrifugal test tube is provided with a test tube cover, the test tube cover is arranged into a disposable rubber leather sheath, and one side of the bevel needle, which is far away from the centrifugal rotor, is provided with a blade.

As an alternative to the centrifugal apparatus for galactomannan experiments described in the present invention, wherein: the centrifugal test tube is placed obliquely, the tube orifice of the centrifugal test tube is adjacent to the center of the centrifugal rotor, the bevel needle is arranged as a bevel needle, one end of the bevel needle, which is adjacent to the liquid taking mechanism, is arranged as a vertical part, the part, which is adjacent to the centrifugal test tube, is arranged as an inclined part, the inclined angle of the bevel needle inclined part is consistent with the inclined angle of the centrifugal test tube, an inner shell is arranged in the upper cover of the centrifugal test tube, a bottom cover is detachably arranged at the bottom of the inner shell, a plurality of outlet needle holes are formed in the bottom cover in an annular array, the bevel needle penetrates through the outlet needle holes, the outlet needle holes are arranged as obliquely-formed perforations, and the inclined angle of the outlet needle holes is consistent with the inclined angle of the centrifugal test tube.

As an alternative to the centrifugal apparatus for galactomannan experiments described in the present invention, wherein: the automatic test tube comprises a centrifugal inner container, a dispatching mounting plate, a dispatching motor, a dispatching driving gear, a clamping column, a tube wall rack, an arc clamping groove, a first gear ring and a second gear ring, wherein the dispatching mounting plate is mounted on the inner wall of the centrifugal inner container, the dispatching motor is mounted on the dispatching mounting plate, the dispatching driving gear is connected with an output shaft of the dispatching motor, the outer wall of the centrifugal test tube is provided with the clamping column and the tube wall rack, the arc clamping groove is formed in a slot of a centrifugal rotor in a sliding fit mode, the first gear ring and the second gear ring are integrally connected below the centrifugal rotor, the first gear ring is located below the second gear ring, the first gear ring is arranged to be an inner gear ring, the first gear ring is meshed with the dispatching driving gear, the second gear ring is arranged to be an outer gear ring, and the second gear ring is meshed with the arc clamping groove.

As an alternative to the centrifugal apparatus for galactomannan experiments described in the present invention, wherein: the centrifugal motor is arranged inside the centrifugal liner, a rotor seat is rotatably arranged at the top of the centrifugal liner, the centrifugal rotor is fixedly connected with the rotor seat, and the centrifugal rotor is simultaneously connected with an output shaft of the centrifugal motor.

The invention has the following beneficial effects:

1. according to the centrifugal equipment for the galactomannan experiment, a centrifugal test tube for the galactomannan experiment is centrifuged through the arrangement of a centrifugal motor and a centrifugal rotating head, layering is generated in the centrifugal test tube, supernatant and sediment are separated, a liquid taking mechanism can move up and down through the cooperation of a liquid taking lifting piece and a liquid taking plate, a bevel needle can be inserted into the centrifugal test tube, the liquid taking pump is operated to enable the upper half part of a liquid taking cylinder to be negative pressure through the cooperation of the liquid taking pump and the liquid taking mechanism, and a piston block moves upwards, so that the lower half part of the liquid taking cylinder is provided with suction force, and supernatant in the centrifugal test tube can be sucked away; therefore, after centrifugation, when the device can automatically complete the operation of removing the supernatant, manual operation is replaced, experimental manual steps are reduced, the supernatant is automatically removed in the centrifugal machine, and the centrifugal test tube does not need to be moved, so that the problem that the supernatant and sediment are mixed when the centrifugal test tube is moved is avoided, the experimental success rate is improved, and the difficulty and pressure of experimental operation are reduced.

2. This centrifugal equipment for galactomannan experiments is put through setting up centrifugal test tube into the slope for deposit can be located the lower position of test tube bottom after centrifuging, namely because the test tube that the slope was put can receive centripetal force in the centrifugation in-process, consequently the deposit can be along the outside deposit of test tube bottom, this overall arrangement makes supernatant be located the opposite side of test tube, this is favorable to separating deposit and supernatant more easily, and furthermore, the centrifugation test tube centrifugation is put to the slope, after the centrifugation finishes, with the manual work in order to be convenient for absorb the supernatant, and the demand of slope centrifugal test tube is unanimous, make the angle syringe needle can more conveniently follow the place imbibition of supernatant gathering, the effect and the efficiency of taking out the supernatant of this equipment have been improved, thereby further reduce the adverse effect of supernatant to galactomannan, the degree of accuracy of experimental result has been improved.

3. This centrifugal equipment for galactomannan experiments, through tilting mechanism's setting, liquid taking mechanism is when descending, first gear and toothed sleeve meshing and rotate, make horizontal propulsion rack promote liquid taking mechanism translation, thereby make liquid taking mechanism remove to the axle center direction when descending, reach liquid taking mechanism tilt movement's effect, make liquid taking mechanism's removal angle the same with centrifugal test tube inclination, thereby realize the dog-ear syringe needle when stretching into centrifugal test tube in the slope, the dog-ear syringe needle is parallel compared in centrifugal test tube, guaranteed the imbibition position of dog-ear syringe needle, the reliability of getting rid of the supernatant and the success rate of experiment have been improved.

4. This centrifugal equipment for galactomannan experiments, through the setting of toothless portion, when taking the liquid board to move down toothless portion, first gear and toothed sleeve remove the meshing for horizontal propulsion rack keeps motionless, lead to the liquid mechanism that gets at this moment only to move down and not translate, thereby make dog-ear syringe needle not go deep along centrifugal test tube's inclination this moment, but horizontal vertical downshift, when first gear is through toothless portion and toothed telescopic rack meshing again, dog-ear syringe needle is moved along centrifugal test tube inner wall, be convenient for dog-ear syringe needle is with centrifugal test tube bottom gathering's supernatant suction, reduce the residual of supernatant, further improve galactomannan experimental result's accuracy.

5. This centrifugal equipment for galactomannan experiments, through the setting of blade, when the dog-ear needle inserts centrifugal test tube, the blade can be with test tube skin lid puncture, when the dog-ear needle needs vertical downshift, the blade can be with test tube skin lid scratch to the dog-ear needle removes.

6. This centrifugal equipment for galactomannan experiments through setting up card post and pipe wall gear strip in the centrifugation test tube outside, when the dog-ear syringe needle removes along the inner wall of centrifugation test tube, dispatch motor starts, through dispatch drive gear, the transmission cooperation of second gear ring, first gear ring, the centrifugation test tube can take place the rotation, because the slip restriction of card post and arc draw-in groove for the centrifugation test tube can slightly reciprocate in the rotation, the whole removal orbit of centrifugation test tube is arc curve and removes like this, the dog-ear syringe needle slides relative centrifugation test tube inner wall when the adherence, the dog-ear syringe needle is imbibited along the wall-hanging edge of supernatant fluid slope arc, thereby reduce the wall-hanging residue of supernatant fluid, further improve the accuracy of galactomannan experimental result.

Drawings

Fig. 1 is a schematic overall perspective view of the present invention.

Fig. 2 is a schematic view of the overall three-dimensional explosion structure of the present invention.

Fig. 3 is a schematic perspective cut-away structure of the present invention.

Fig. 4 is a schematic cross-sectional view of the upper cover of the present invention.

FIG. 5 is a schematic diagram showing the structure of the centrifugation tube of the present invention when the centrifugation tube starts to aspirate the supernatant.

FIG. 6 is a schematic diagram showing the structure of the centrifugation tube of the present invention at the end of the supernatant aspiration.

Fig. 7 is an enlarged schematic view of the structure of the present invention at a.

Fig. 8 is an enlarged schematic view of the structure of the present invention at B.

Fig. 9 is a schematic diagram of the structure of the present invention at C before operation.

Fig. 10 is a schematic diagram of the structure at runtime C of the present invention.

Fig. 11 is an enlarged view of the structure of the present invention at D.

Fig. 12 is a schematic view of an arc-shaped slot structure of the present invention.

In the figure: 110. a centrifuge housing; 120. a centrifuge upper cover; 130. a liquid taking lifting part; 140. a liquid taking air pump; 141. a liquid taking air pipe; 150. centrifuging the inner container; 160. centrifuging the rotor; 170. a centrifugal motor; 180. a rotor seat; 190. centrifuging the test tube; 191. a test tube cover; 200. a liquid taking plate; 201. penetrating a groove; 210. a chute; 220. a toothed sleeve; 221. a toothless portion; 230. an inner case; 240. a bottom cover; 250. a pinhole is formed; 260. a limit column; 300. a liquid taking mechanism; 310. a liquid taking cylinder; 320. sealing the upper cover; 330. a piston block; 340. a through-flow port; 350. an outer wrapping ring; 400. a bevel needle; 410. a blade; 500. a tilting mechanism; 510. a first gear; 520. a second gear; 530. a transverse pushing rack; 540. a sliding seat; 610. dispatching a motor; 620. scheduling a mounting plate; 630. scheduling a drive gear; 640. a first gear ring; 650. a second gear ring; 660. a clamping column; 670. a pipe wall rack; 680. an arc-shaped clamping groove.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

First, this embodiment is intended to facilitate solving the problem that the progress and result of the experiment are affected by taking a long time if there are several groups of control groups in the experiment by manually removing the supernatant using a pipette with care, and referring to fig. 1 to 12, a centrifugal apparatus for galactomannan experiments includes a centrifuge housing 110, a centrifuge upper cover 120 mounted on top of the centrifuge housing 110, a centrifuge rotor 160 for centrifuging the material, a centrifuge tube 190 mounted on the centrifuge rotor 160, a tube cap 191 mounted on a tube opening of the centrifuge tube 190, and a centrifuge motor 170 for driving the centrifuge rotor 160 to rotate, a liquid taking lifter 130 and a liquid taking pump 140 mounted on the centrifuge upper cover 120, and the liquid taking lifter 130 is configured as a cylinder or an electric lifting table.

The liquid taking plate 200 is connected to the lifting end of the liquid taking lifting member 130, and a liquid taking mechanism 300 for sucking the supernatant liquid into the centrifugal test tube 190 is mounted on the liquid taking plate 200. In the specific arrangement, see fig. 2, the centrifuge rotor 160 is provided with an even number of pairs of tube slots, which are diagonally positioned when the centrifuge tube 190 is placed, because weight balance is important in centrifugation. The rotor of the centrifuge has a high dynamic energy in high speed motion. If not properly balanced, the entire centrifugal unit can be dislodged from its position, causing serious damage.

The centrifugal shell 110 internally mounted has centrifugal inner bag 150, and centrifugal motor 170 installs inside centrifugal inner bag 150, and rotor seat 180 is installed in the top rotation of centrifugal inner bag 150, and centrifugal rotor 160 and rotor seat 180 fixed connection, rotor seat 180 pass through the inner wall sliding fit of bearing and centrifugal inner bag 150, and such setting for centrifugal rotor 160 is more stable when rotatory, has improved centrifugal stability and the effect. The centrifugal rotor 160 is simultaneously connected to an output shaft of a centrifugal motor 170.

In the galactomannan experiment, 0.6. 0.6 g galactomannan powder is weighed in 15 mL DMEM culture medium, mixed evenly by shaking, filtered by a 0.22 mu m filter membrane, and the prepared galactomannan mother liquor concentration is 40 g/L, and the dosing concentration in the subsequent experiment is directly diluted by the mother liquor. In this example, the cell growth medium used was: 10% fetal bovine serum, 1% penicillin streptomycin diabody solution and 89% DMEM medium. After the cells were well adhered to the bottom wall in the T25 vial, the cells were washed once with 1 mL PBS. Adding 1 mL pancreatin digestion solution, digesting the cells for 1 min, and observing the cells under a mirror to turn round and lighten. Digestion was stopped by adding 2 mL cell growth medium, gently transferred to a 15 mL centrifuge tube, and centrifuged at 1200 r/min for 5 min. The supernatant was discarded, cells were seeded into 96-well plates at 6000 cells/well, dosing medium containing 1.6, 3.2, 6.4. 6.4 g/L galactomannan was prepared using stock solution and added to the plates while a blank, i.e., cell growth medium, was set and incubated in an incubator.

In order to remove the supernatant more efficiently, as shown in fig. 8, the liquid taking mechanism 300 includes a liquid taking barrel 310 engaged with the liquid taking plate 200, a piston block 330 slidably disposed inside the liquid taking barrel 310, and a through hole 340 disposed at the bottom of the liquid taking barrel 310, where a bevel needle 400 is detachably inserted outside the through hole 340. The bevel needle 400 may be provided as a disposable needle.

The top of the liquid taking barrel 310 is provided with a sealing upper cover 320, a sealing space is formed above a piston block 330 inside the liquid taking barrel 310 through the arrangement of the sealing upper cover 320, the sealing upper cover 320 is connected with a liquid taking air pipe 141, the other end of the liquid taking air pipe 141 is connected with the air suction end of the liquid taking air pump 140, the piston block 330 is in sliding fit with the inner wall of the liquid taking barrel 310, a plurality of sliding grooves 210 are formed in an annular array on the liquid taking plate 200, and the liquid taking barrel 310 is in sliding fit with the sliding grooves 210.

Fig. 4, a plurality of spacing posts 260 are fixedly installed on the bottom side of the upper cover 120 of the centrifugal machine, the plurality of spacing posts 260 are vertically arranged, the spacing posts 260 penetrate through the liquid taking plate 200, the liquid taking plate 200 is in sliding fit with the spacing posts 260, and through the arrangement of the spacing posts 260, the liquid taking plate 200 is more stable when moving, shaking does not easily occur, and the liquid taking precision of equipment is improved.

In this embodiment: through the arrangement of the centrifugal motor 170 and the centrifugal rotor 160, the centrifugal test tube 190 for galactomannan experiments is centrifuged, layering is generated in the centrifugal test tube 190, supernatant and sediment are separated, the liquid taking mechanism 300 can move up and down through the cooperation of the liquid taking lifting piece 130 and the liquid taking plate 200, so that the angle-folding needle 400 can be inserted into the centrifugal test tube 190, and through the cooperation of the liquid taking pump 140 and the liquid taking mechanism 300, the liquid taking pump 140 operates to enable the upper half part of the liquid taking cylinder 310 to be in negative pressure, and the piston block 330 moves upwards, so that the lower half part of the liquid taking cylinder 310 has suction force, and supernatant in the centrifugal test tube 190 can be sucked away; therefore, after centrifugation, when the device can automatically complete the operation of removing the supernatant, manual operation is replaced, experimental manual steps are reduced, the supernatant is automatically removed in the centrifuge, and the centrifugal test tube 190 does not need to be moved, so that the problem that the supernatant and sediment are mixed when the centrifugal test tube 190 is moved is avoided, the experimental success rate is improved, and the difficulty and pressure of experimental operation are reduced.

In a second embodiment, the present embodiment is intended to facilitate solving the problem that the supernatant residue needs to be further reduced, and the present embodiment is an improvement made on the basis of the first embodiment, specifically, referring to fig. 1-12, the centrifugal test tube 190 is disposed obliquely, the nozzle of the centrifugal test tube 190 is adjacent to the center of the centrifugal rotor 160, the bevel needle 400 is configured as a bevel needle, one end of the bevel needle 400 adjacent to the liquid taking mechanism 300 is configured as a vertical portion, the portion adjacent to the centrifugal test tube 190 is configured as an oblique portion, and the oblique angle of the oblique portion of the bevel needle 400 is consistent with the oblique angle of the centrifugal test tube 190. Through setting up centrifugal test tube 190 into the slope and put for the deposit can be located the lower position in test tube bottom after centrifuging, namely because the test tube that the slope put can receive centripetal force effect in the centrifugation in-process, consequently the deposit can be along the one side deposit of keeping away from the rotation axis in test tube bottom outside, this overall arrangement makes supernatant be located the opposite side of test tube, this is favorable to separating deposit and supernatant more easily, and not only this, the slope puts centrifugal test tube 190 centrifugation, after the centrifugation finishes, with the manual work in order to be convenient for absorb the supernatant, and the demand of slope centrifugal test tube 190 is unanimous, make dog-ear needle 400 can more conveniently follow the place imbibition of supernatant gathering, the effect and the efficiency of taking out the supernatant of this equipment have been improved, thereby further reduce the adverse effect of supernatant on galactomannan, the degree of accuracy of experimental result has been improved.

Referring to fig. 8, an inner casing 230 is disposed in the centrifuge upper cover 120, a bottom cover 240 is detachably mounted at the bottom of the inner casing 230, a plurality of pin holes 250 are formed in an annular array on the bottom cover 240, the bevel pins 400 penetrate the pin holes 250, the pin holes 250 are formed as obliquely-formed perforations, and the inclination angle of the pin holes 250 is consistent with that of the centrifuge test tube 190. After centrifugation or experimentation, cleaning of the device and replacement of the bevel needle 400 can be accomplished by removal of the bottom cap 240.

The liquid taking plate 200 is arranged as a circular plate, the toothed sleeve 220 is arranged inside the upper cover 120 of the centrifugal machine, the toothed sleeve 220 is arranged as an annular sleeve with rack distributed on the inner wall, the toothed sleeve 220 and the liquid taking plate 200 are coaxially arranged, a plurality of through grooves 201 are formed in an annular array on the edge of the liquid taking plate 200, and an inclined mechanism 500 for driving the liquid taking mechanism 300 to lift and translate simultaneously is arranged in the through grooves 201.

The tilting mechanism 500 comprises a first gear 510 and a second gear 520 which are rotatably installed in the through groove 201, the first gear 510 is meshed with racks of the toothed sleeve 220, the second gear 520 is meshed with the first gear 510, a sliding seat 540 is installed at the position, adjacent to the through groove 201, of the liquid taking plate 200, a transverse pushing rack 530 is installed on the sliding seat 540 in a sliding mode, the second gear 520 is meshed with the transverse pushing rack 530, and an outer wrapping ring 350 fixedly connected with the liquid taking mechanism 300 is installed at the end portion of the transverse pushing rack 530. Specifically, the outer ring 350 is engaged with the outer side of the liquid taking tube 310.

The trough 201, the tilting mechanism 500 and the liquid taking mechanism 300 are arranged in the same number and at the same angle.

In this embodiment: through the setting of tilting mechanism 500, liquid taking mechanism 300 is when descending, first gear 510 meshes with toothed sleeve 220 and rotates, make transversely impel rack 530 and promote liquid taking mechanism 300 translation, thereby make liquid taking mechanism 300 remove to the axle center direction when descending, reach liquid taking mechanism 300 tilt movement's effect, make liquid taking mechanism 300's removal angle and centrifugal test tube 190 inclination the same, thereby realize dog-ear needle 400 when stretching into centrifugal test tube 190 in the slope, dog-ear needle 400 is parallel compared with centrifugal test tube 190, the imbibition position of dog-ear needle 400 has been ensured, the reliability of getting rid of the supernatant and the success rate of experiment have been improved.

In the third embodiment, in order to facilitate solving the problem that the supernatant liquid is collected at the bottom of the test tube and is inconvenient to suck in the later period of the supernatant liquid suction, the present embodiment is an improvement made on the basis of the second embodiment, specifically, referring to fig. 1 to 12, the inner wall of the toothed sleeve 220 is provided with a toothless portion 221, the toothless portion 221 is provided with a smooth inner wall without a rack, and when the liquid taking plate 200 corresponds to the toothless portion 221, the first gear 510 is in clearance fit with the toothless portion 221.

Referring to fig. 5 and 6, the test tube cover 191 is configured as a disposable rubber boot, and the side of the bevel needle 400 remote from the centrifuge head 160 is provided with a blade 410.

By the arrangement of the blade 410, the blade 410 can puncture the tube cover 191 when the bevel needle 400 is inserted into the centrifugal tube 190, and the blade 410 can puncture the tube cover 191 when the bevel needle 400 needs to be moved vertically downward, so that the bevel needle 400 can be moved.

In this embodiment: through the setting of toothless portion 221, when liquid taking plate 200 moves down to toothless portion 221, first gear 510 and toothed sleeve 220 are disengaged for transversely impel rack 530 to keep motionless, lead to the liquid taking mechanism 300 of this moment only to move down and not translate, thereby make dog-ear needle 400 not go deep along centrifugal test tube 190's inclination this moment, but horizontal vertical downshift, when first gear 510 passes toothless portion 221 again and toothed sleeve 220's rack engagement, dog-ear needle 400 moves along centrifugal test tube 190 inner wall, dog-ear needle 400 is convenient for suck away centrifugal test tube 190 bottom gathering supernatant, reduce the residual of supernatant, further improve galactomannan experimental result's accuracy.

In the fourth embodiment, the solution to the problem of sucking the supernatant onto the wall is intended to be improved, and the solution is an improvement based on the third embodiment, specifically, referring to fig. 1 to 12, a dispatching installation plate 620 is installed on the inner wall of the centrifugal liner 150, a dispatching motor 610 is installed on the dispatching installation plate 620, an output shaft of the dispatching motor 610 is connected with a dispatching driving gear 630, a clamping column 660 and a pipe wall rack 670 are provided on the outer wall of the centrifugal test tube 190, an arc-shaped clamping groove 680 in sliding fit with the clamping column 660 is provided at a slot of the centrifugal rotor 160, a first gear ring 640 and a second gear ring 650 are integrally connected below the centrifugal rotor 160, the first gear ring 640 is located below the second gear ring 650, the first gear ring 640 is set as an inner gear ring, the first gear ring 640 is meshed with the dispatching driving gear 630, the second gear ring 650 is set as an outer gear ring, and the second gear ring 650 is meshed with the arc-shaped clamping groove 680.

The second gear ring 650 is always engaged with the arcuate catch groove 680 as the centrifuge tube 190 is displaced up and down from the rest.

Referring to fig. 7, in order to facilitate placement of the centrifugal test tube 190, the centrifugal rotor 160 is configured as a separate structure that is clamped up and down, and when the centrifugal test tube 190 is placed, the upper cover is opened, the pipe wall rack 670 is clamped with the second gear ring 650, and after the clamping column 660 is abutted with the arc-shaped clamping groove 680, the upper cover is clamped again, so as to fix the centrifugal test tube 190.

In this embodiment: through set up draw-in column 660 and pipe wall rack 670 in centrifugal test tube 190 outside, when dog-ear needle 400 moves along centrifugal test tube 190's inner wall, dispatch motor 610 starts, through dispatch drive gear 630, second gear ring 650, first gear ring 640's transmission cooperation, centrifugal test tube 190 can take place the rotation, because draw-in column 660 and the slip restriction of arc draw-in groove 680 for centrifugal test tube 190 can slightly reciprocate in the rotation, so centrifugal test tube 190 whole moving track is the arc curve and removes, make dog-ear needle 400 slide relative centrifugal test tube 190 inner wall curve when the adherence, dog-ear needle 400 is imbibed along the slope arc edge of supernatant fluid, thereby reduce the wall built-up residual of supernatant fluid, further improve the accuracy of galactomannan experimental result.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

The foregoing is merely a preferred embodiment of the present invention, and it should be noted that it will be apparent to those skilled in the art that several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the scope of the invention.

Claims (10)

1. The utility model provides a centrifugal equipment for galactomannan experiments, includes centrifuge shell (110), installs centrifuge upper cover (120) at centrifuge shell (110) top, is used for centrifuging to material centrifugal rotor (160), cartridge centrifugal test tube (190) on centrifugal rotor (160), and be used for driving centrifugal rotor (160) pivoted centrifugal motor (170), its characterized in that: the centrifugal machine upper cover (120) is provided with a liquid taking lifting piece (130) and a liquid taking air pump (140), the lifting end of the liquid taking lifting piece (130) is connected with a liquid taking plate (200), and the liquid taking plate (200) is provided with a liquid taking mechanism (300) for sucking supernatant in the centrifugal test tube (190);

the liquid taking mechanism (300) comprises a liquid taking cylinder (310) clamped with the liquid taking plate (200), a piston block (330) slidably arranged in the liquid taking cylinder (310), and a through hole (340) arranged at the bottom of the liquid taking cylinder (310), wherein a bevel needle (400) is detachably arranged outside the through hole (340).

2. A centrifugal device for galactomannan experiments according to claim 1, wherein: the utility model discloses a liquid collecting device, including a liquid collecting tube (310), a liquid collecting plate (200), a piston block (330), a sealing upper cover (320) is installed at liquid collecting tube (310) top, through the setting of sealing upper cover (320), liquid collecting tube (310) is inside the top of piston block (330) forms sealed space, sealing upper cover (320) is connected with gets liquid trachea (141), the other end of getting liquid trachea (141) with get the suction end of liquid pump (140) and be connected, piston block (330) with get liquid tube (310) inner wall slip laminating, annular array is provided with a plurality of spouts (210) on liquid collecting plate (200), liquid collecting tube (310) with spout (210) sliding fit.

3. A centrifugal device for galactomannan experiments according to claim 1, wherein: the centrifugal machine upper cover (120) bottom side fixed mounting has a plurality of spacing posts (260), and is a plurality of spacing posts (260) are vertical setting, spacing post (260) run through get liquid board (200), get liquid board (200) with spacing post (260) sliding fit.

4. A centrifugal device for galactomannan experiments according to claim 1, wherein: get liquid board (200) and set up to the circular plate, centrifuge upper cover (120) inside is provided with toothed sleeve (220), toothed sleeve (220) set up to the annular sleeve of inner wall distribution rack, toothed sleeve (220) with get liquid board (200) coaxial setting, get a plurality of grooves (201) of wearing have been seted up to liquid board (200) edge annular array, wear to be provided with in groove (201) and be used for driving get tilting mechanism (500) of liquid mechanism (300) lift translation simultaneously.

5. A centrifugal device for galactomannan experiments as claimed in claim 4, wherein: the tilting mechanism (500) is including rotating first gear (510) and second gear (520) of installing in wearing groove (201), first gear (510) with the rack-engaging of taking tooth sleeve (220), second gear (520) with first gear (510) meshing, get liquid board (200) adjacent wearing groove (201) department installs sliding seat (540), sliding mounting has horizontal propulsion rack (530) on sliding seat (540), second gear (520) with horizontal propulsion rack (530) meshing, the tip of horizontal propulsion rack (530) install with get outer packing ring (350) of liquid mechanism (300) fixed connection.

6. A centrifugal device for galactomannan experiments as claimed in claim 5, wherein: the inner wall of the toothed sleeve (220) is provided with a toothless part (221), the toothless part (221) is provided with a smooth inner wall without a rack, and when the liquid taking plate (200) corresponds to the toothless part (221), the first gear (510) is in clearance fit with the toothless part (221).

7. A centrifugal device for galactomannan experiments according to claim 1, wherein: the tube mouth of the centrifugal test tube (190) is provided with a test tube leather cover (191), the test tube leather cover (191) is arranged into a disposable rubber leather cover, and one side of the bevel needle (400) far away from the centrifugal rotor (160) is provided with a blade (410).

8. A centrifugal device for galactomannan experiments according to claim 1, wherein: the centrifugal test tube (190) is obliquely arranged, a tube orifice of the centrifugal test tube (190) is adjacent to the center of the centrifugal rotor (160), the bevel needle (400) is provided with a bevel needle, one end of the bevel needle (400) adjacent to the liquid taking mechanism (300) is provided with a vertical part, the part adjacent to the centrifugal test tube (190) is provided with an inclined part, and the inclined angle of the inclined part of the bevel needle (400) is consistent with the inclined angle of the centrifugal test tube (190);

the centrifugal tube is characterized in that an inner shell (230) is arranged in the upper cover (120) of the centrifugal machine, a bottom cover (240) is detachably arranged at the bottom of the inner shell (230), a plurality of pin holes (250) are formed in an annular array on the bottom cover (240), the bevel pins (400) penetrate through the pin holes (250), the pin holes (250) are obliquely formed through holes, and the inclination angle of the pin holes (250) is consistent with that of the centrifugal tube (190).

9. A centrifugal device for galactomannan experiments according to claim 1, wherein: centrifuge shell (110) internally mounted has centrifugal inner bag (150), dispatch mounting panel (620) are installed to centrifugal inner bag (150) inner wall, install dispatch motor (610) on dispatch mounting panel (620), the output shaft of dispatch motor (610) has dispatch drive gear (630), centrifugal test tube (190) outer wall is provided with draw-in post (660) and pipe wall rack (670), slot department of centrifugal rotor (160) seted up with draw-in post (660) sliding fit's arc draw-in groove (680), centrifugal rotor (160) below integration is connected with first gear ring (640) and second gear ring (650), first gear ring (640) are located the below of second gear ring (650), first gear ring (640) set up to the internal gear ring, first gear ring (640) with dispatch drive gear (630) meshing, second gear ring (650) set up to the external gear ring, second gear ring (650) with arc draw-in groove (680) meshing.

10. A centrifugal device for galactomannan experiments according to claim 1, wherein: the centrifugal motor (170) is arranged inside the centrifugal liner (150), a rotor seat (180) is rotatably arranged at the top of the centrifugal liner (150), the centrifugal rotor (160) is fixedly connected with the rotor seat (180), and the centrifugal rotor (160) is simultaneously connected with an output shaft of the centrifugal motor (170).