CN115024441A - Feed additive for promoting immunity improvement of grass carp and application thereof - Google Patents

Feed additive for promoting immunity improvement of grass carp and application thereof Download PDF

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Publication number
CN115024441A
CN115024441A CN202210739309.7A CN202210739309A CN115024441A CN 115024441 A CN115024441 A CN 115024441A CN 202210739309 A CN202210739309 A CN 202210739309A CN 115024441 A CN115024441 A CN 115024441A
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parts
feed additive
grass carp
immunity
promoting
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CN202210739309.7A
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刘飞
罗智
薛春雨
居伟
彭继伟
王爱民
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Tongwei Dafeng Feed Co ltd
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Tongwei Dafeng Feed Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Physiology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Insects & Arthropods (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Birds (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)

Abstract

The invention relates to the technical field of fish feed, and discloses a feed additive for promoting the immunity of grass carps to be improved and application thereof, wherein the feed additive for promoting the immunity of the grass carps to be improved is prepared from the following components in parts by weight: DDGS5-10 parts, broad bean protein 5-10 parts, dandelion 0.15-0.35 part, garlic and ginger powder 0.05-0.25 part, agrocybe cylindracea 0.01-0.05 part, berberis 0.01-0.05 part, alpha-amylase 0.01-0.05 part, wild chrysanthemum flower 0.05-0.15 part, alternanthera philoxeroides 2-5 parts and spirulina 1-3 parts. According to the feed additive for promoting the immunity of the grass carps to be improved and the application thereof, the feed additive for promoting the immunity of the grass carps to be improved is combined with DDGS, broad bean protein, dandelion, agrocybe cylindracea, berberis thunbergii, wild chrysanthemum flower, alternanthera philoxeroides and spirulina by utilizing the synergistic effect of the garlic and ginger powder and the alpha-amylase, so that the immunity of the grass carps can be enhanced, and the survival rate of the grass carps is ensured without adding hormones.

Description

Feed additive for promoting immunity improvement of grass carp and application thereof
Technical Field
The invention relates to the technical field of fish feed, in particular to a feed additive for promoting the immunity of grass carp to be improved and application thereof.
Background
Grass carp: ctenophagostin idellus is fish belonging to Cyprinaceae and grass carp, is an important freshwater fish for aquaculture in China, and forms four major Chinese carps together with silver carp, bighead carp and black carp. Grass carp has been cultivated for over 1700 years, but before the grass carp is eaten or sold after natural fry in rivers are cultivated in a pond, and the artificial cultivation is really realized from the artificial propagation of four Chinese carps in 1958.
With the development of pollution-free sustainable culture and green, efficient and pollution-free fishery medicine, the research on green ecological, residual pollution-free and pollution-free immunopotentiators becomes the research focus of the aquaculture industry at present. The phenomenon of drug abuse in the existing culture process is serious, and the abuse and the residue of chemical drugs such as antibiotics and the like can not only endanger the culture of the shrimps and crabs, but also endanger the whole shrimp and crab culture industry.
Therefore, we propose a feed additive for promoting the immunity improvement of grass carp and its application to solve the above problems.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the feed additive for promoting the immunity of the grass carp to be improved and the application thereof, has the advantages of enhancing the immunity of the grass carp, having high survival rate, fast growth of the body mass of the fish and the like, and solves the problem that chemical drugs such as antibiotics and the like are abused.
(II) technical scheme
In order to realize the aim of enhancing the immunity of the grass carp, having high survival rate and fast growth of fish body mass, the invention provides the following technical scheme: the feed additive for enhancing the immunity of the grass carps comprises the following components in parts by mass: DDGS5-10 parts, broad bean protein 5-10 parts, dandelion 0.15-0.35 part, garlic and ginger powder 0.05-0.25 part, agrocybe cylindracea 0.01-0.05 part, berberis 0.01-0.05 part, alpha-amylase 0.01-0.05 part, wild chrysanthemum flower 0.05-0.15 part, alternanthera philoxeroides 2-5 parts and spirulina 1-3 parts.
Preferably, the feed additive for promoting the immunity of the grass carp to be improved is prepared from the following components in parts by weight: DDGS5 parts, broad bean protein 5 parts, dandelion 0.15 parts, garlic and ginger powder 0.05 parts, tea mushroom 0.01 parts, barberry 0.01 parts, alpha-amylase 0.01 parts, wild chrysanthemum flower 0.05 parts, water peanut 2 parts and spirulina 1 part.
Preferably, the feed additive for promoting the immunity of the grass carp to be improved is prepared from the following components in parts by weight: DDGS10 parts, broad bean protein 10 parts, dandelion 0.35 parts, garlic and ginger powder 0.25 parts, tea mushroom 0.05 parts, barberry 0.05 parts, alpha-amylase 0.05 parts, wild chrysanthemum flower 0.15 parts, water peanut 5 parts and spirulina 3 parts.
Preferably, the feed additive for promoting the immunity of the grass carp to be improved is prepared from the following components in parts by weight: DDGS6 parts, broad bean protein 8 parts, dandelion 0.2 parts, garlic and ginger powder 0.15 parts, agrocybe aegerita 0.02 parts, barberry 0.04 parts, alpha-amylase 0.03 parts, wild chrysanthemum flower 0.1 parts, alternanthera philoxeroides 4 parts and spirulina 2 parts.
Preferably, the doping amount of the feed additive is 2% -6% of the grass carp feed.
Preferably, the doping amount of the feed additive is 3% of the grass carp feed.
(III) advantageous effects
Compared with the prior art, the invention provides the feed additive for promoting the immunity of the grass carp to be improved and the application thereof, and the feed additive has the following beneficial effects:
1. the feed additive for promoting immunity improvement of grass carp and the application thereof are characterized in that DDGS, broad bean protein, dandelion, garlic ginger powder, agrocybe cylindracea, berberis thunbergii, alpha-amylase, wild chrysanthemum flower, alternanthera philoxeroides and spirulina are adopted, and the synergistic effect of the garlic ginger powder and the alpha-amylase is utilized to be combined with DDGS, broad bean protein, dandelion, agrocybe cylindracea, barberry, wild chrysanthemum flower, alternanthera philoxeroides and spirulina, so that the immunity of the freshwater fish can be enhanced, and the survival rate of the grass carp is ensured without adding hormone.
Detailed Description
Example 1
The feed additive for promoting grass carp immunity improvement is prepared from the following components in parts by weight: DDGS5 parts, broad bean protein 5 parts, dandelion 0.15 parts, garlic and ginger powder 0.05 parts, tea mushroom 0.01 parts, barberry 0.01 parts, alpha-amylase 0.01 parts, wild chrysanthemum flower 0.05 parts, water peanut 2 parts and spirulina 1 part.
The raw material products are selected from the raw materials sold in the market, wherein dandelion, agrocybe aegerita, berberis thunbergii, wild chrysanthemum, alternanthera philoxeroides and spirulina are cleaned by deionized water, dried in the shade, ground into 80-100 meshes, uniformly mixed with DDGS, broad bean protein, garlic and ginger powder and alpha-amylase, doped according to 5% of the conventional feed of grass carp feed, and processed into the commercial feed according to the conventional processing mode of the grass carp feed.
The experimental grass carp was domesticated in a water pond for 2 weeks, during which commercial grass carp juvenile feed (crude protein content 32%, crude fat content 4%) was fed. After 24h of hunger treatment, selecting individuals with strong physique and similar physique and weighing, setting 3 times of hunger treatment, randomly distributing the hungry test grass carps into 800L water tanks, and stocking 20 test grass carps with initial body mass of (57.11 +/-0.19) g in each water tank. The feed additive and commercial grass carp juvenile feed adulterants (8:00 and 16:00) in the embodiment are fed for 2 times per day of apparent satiation, and natural illumination is adopted in the period, and air inflation and oxygenation are ceaselessly performed. The culture experiment lasts for 60 d. At the beginning of the culture experiment, 5 fish were randomly picked and stored at-80 ℃ for analysis of the body composition of the initial sample. At the end of the culture experiment, the experimental fish are fasted for 24h, and the fish tails and the total mass of each water tank are counted. Then randomly taking 1mL of blood from 5 experimental fish veins, standing the blood at 4 ℃ for 4h, centrifuging (836 Xg, 10min, 4 ℃), and taking serum for measuring immunological index. Through determination, the survival rate of the experimental fish is 100%, the mass of the terminal body is 131 +/-0.17 g, the activity of the serum lysozyme is 8.82 +/-0.93 unit/mu L, and the content of the serum protein is 52.11 +/-1.21 mg/mL.
Example 2
The feed additive for promoting the immunity of the grass carps to be improved comprises the following components in parts by weight: DDGS10 parts, broad bean protein 10 parts, dandelion 0.35 parts, garlic and ginger powder 0.25 parts, tea mushroom 0.05 parts, barberry 0.05 parts, alpha-amylase 0.05 parts, wild chrysanthemum flower 0.15 parts, water peanut 5 parts and spirulina 3 parts.
The above raw material products are all selected from the commercial products. Wherein, the dandelion, the agrocybe aegerita, the berberis thunbergii, the wild chrysanthemum, the alternanthera philoxeroides and the spirulina are cleaned by deionized water, dried in the shade, ground into 80-100 meshes, uniformly mixed with the DDGS, the broad bean protein, the garlic ginger powder and the alpha-amylase, doped according to 4 percent of the conventional feed of the grass carp feed, and processed into the commercial feed according to the conventional processing mode of the grass carp feed. .
The experimental fish were domesticated in the pond for 2 weeks, during which commercial young grass carp feed (crude protein content 32%, crude fat content 4%) was fed. After 24h of hunger treatment, selecting individuals with strong physique and similar physique and weighing, setting 3 times of hunger treatment, randomly distributing the hunger treatment to 800L water tanks, and stocking 20 tails of experimental fishes with initial body mass of (57.29 +/-0.17) g in each water tank. The feed additive and commercial grass carp juvenile feed adulterants (8:00 and 16:00) in the embodiment are fed for 2 times every day apparent after being fed fully, and natural illumination is adopted during the period, and the continuous aeration and oxygenation are carried out. The culture experiment lasts for 60 days. At the beginning of the culture experiment, 5 fish were randomly picked and stored at-80 ℃ for analysis of the body composition of the initial sample. At the end of the culture experiment, the experimental fish are fasted for 24h, and the fish tails and the total mass of each water tank are counted. Then, 1mL of blood is randomly taken from the vein of 5 experimental fishes, the blood is kept still for 4 hours at the temperature of 4 ℃, and then the blood is centrifuged (800 Xg, 10min, 4 ℃), and serum is taken for measuring the immunological index. Through determination, the survival rate of the experimental fish is 100%, the mass of the final body is 129.31 +/-0.22 g, the activity of the serum lysozyme is 8.79 +/-0.71 unit/mu L, and the content of the serum protein is 50.81 +/-1.42 mg/mL.
Example 3
The feed additive for promoting grass carp immunity improvement is prepared from the following components in parts by mass: DDGS6 parts, broad bean protein 8 parts, dandelion 0.2 parts, garlic and ginger powder 0.15 parts, tea mushroom 0.02 parts, barberry 0.04 parts, alpha-amylase 0.03 parts, wild chrysanthemum flower 0.1 parts, alternanthera philoxeroides 4 parts and spirulina 2 parts.
The above raw material products are all selected from the commercial products. Wherein, the dandelion, the agrocybe aegerita, the berberis thunbergii, the wild chrysanthemum, the alternanthera philoxeroides and the spirulina are cleaned by deionized water, dried in the shade, ground into 80-100 meshes, uniformly mixed with the DDGS, the broad bean protein, the garlic ginger powder and the alpha-amylase, doped according to 3 percent of the conventional feed of the grass carp feed, and processed into the commercial feed according to the conventional processing mode of the grass carp feed.
The experimental fish were kept in a water tank for 2 weeks, during which commercial young grass carp feed (crude protein content 32%, crude fat content 4%) was fed. After 24h of hunger treatment, selecting individuals with strong physique and similar physique and weighing, setting 3 times of hunger treatment, randomly distributing the hunger treatment to 800L water tanks, and stocking 20 experimental fishes of which the initial body mass is (56.12 +/-0.14) g in each water tank. The feed additive and commercial grass carp juvenile feed adulterants (8:00 and 16:00) in the embodiment are fed for 2 times per day of apparent satiation, and natural illumination is adopted in the period, and air inflation and oxygenation are ceaselessly performed. The culture experiment lasts for 60 d. At the beginning of the culture experiment, 5 fish were randomly picked and stored at-80 ℃ for analysis of the body composition of the initial sample. At the end of the culture experiment, the experimental fish are fasted for 24h, and the fish tails and the total mass of each water tank are counted. Then, 1mL of blood is randomly taken from the vein of 5 experimental fishes, the blood is kept still for 4 hours at the temperature of 4 ℃, and then the blood is centrifuged (800 Xg, 10min, 4 ℃), and serum is taken for measuring the immunological index. Through determination, the survival rate of the experimental fish is 100%, the mass of the terminal body is 141 +/-0.38 g, the activity of the serum lysozyme is 9.38 +/-0.42 unit/mu L, and the content of the serum protein is 55.22 +/-1.08 mg/mL.
Comparative example 1
The pure commercial young grass carp feed (the crude protein content is 32 percent, and the crude fat content is 4 percent) is adopted.
The experimental fish were kept in a water tank for 2 weeks, during which commercial young grass carp feed (crude protein content 32%, crude fat content 4%) was fed. After the domesticated experimental fish is subjected to starvation treatment for 24 hours, individuals with strong physique and similar physique are selected, grouped and weighed, 3 times of treatment are set, the individuals are randomly distributed into 800L water tanks, and 15 tails of the experimental fish with the initial body mass of (57.12 +/-0.19) g are stocked in each water tank. Commercial young grass carp feeds (8:00 and 16:00) sold in the embodiment are fed for 2 times every day after apparent feeding, and natural illumination is adopted during the feeding period, and the feeding is continuously carried out through aeration and oxygenation. The culture experiment lasts for 60 days. At the beginning of the breeding experiment, 5 fish were randomly selected and stored at-80 ℃ for analysis of the body composition of the initial sample. And (4) after the culture experiment is finished, fasting the experimental fish for 24 hours, and counting the fish tails and the total mass of each water tank. Then, 1mL of blood was randomly sampled from the vein of 5 experimental fishes, and the blood was left standing at 4 ℃ for 4 hours and centrifuged (836 Xg, 10min, 4 ℃) to obtain serum for measuring immunological index. Through determination, the survival rate of the experimental fish is 100%, the mass of the final body is 120.87 +/-0.26 g, the activity of the serum lysozyme is 5.53 +/-0.11 unit/mu L, and the content of the serum protein is 42.63 +/-0.21 mg/mL.
Comparative example 2
The difference from example 3 is that no garlic ginger powder and no alpha-amylase are added.
Experiments were conducted in Tongwei feed Limited in Jiangsu salt City. The experimental fish were domesticated in the pond for 2 weeks, during which commercial young grass carp feed (crude protein content 32%, crude fat content 4%) was fed. After the domesticated experimental fish is subjected to starvation treatment for 24 hours, selecting individuals with strong physique and similar physique and weighing, setting 3 times of the starvation treatment, randomly distributing the starvation treatment to 800L water tanks, and stocking 15 tails of the experimental fish with initial body mass of (56.12 +/-0.14) g in each water tank. The feed additive and commercial grass carp juvenile feed adulterants (8:00 and 16:00) in the embodiment are fed for 2 times per day of apparent satiation, and natural illumination is adopted in the period, and air inflation and oxygenation are ceaselessly performed. The culture experiment lasts for 60 days. At the beginning of the culture experiment, 5 fish were randomly picked and stored at-80 ℃ for analysis of the body composition of the initial sample. At the end of the culture experiment, the experimental fish are fasted for 24h, and the fish tails and the total mass of each water tank are counted. Then randomly taking 1mL of blood from 5 experimental fish veins, standing the blood at 4 ℃ for 4h, centrifuging (836 Xg, 10min, 4 ℃), and taking serum for measuring immunological index. Through determination, the survival rate of the experimental fish is 100%, the mass of the final body is 126 +/-0.32 g, the activity of the serum lysozyme is 5.78 +/-0.24 unit/mu L, and the content of the serum protein is 43.38 +/-0.68 mg/mL.
In conclusion, the feed additive for promoting the immunity of the grass carps to be improved and the application thereof are characterized in that DDGS, broad bean protein, dandelion, garlic ginger powder, agrocybe aegerita, berberis thunbergii, alpha-amylase, wild chrysanthemum flower, alternanthera philoxeroides and spirulina are adopted, the synergistic effect of the garlic ginger powder and the alpha-amylase is utilized, and the DDGS, broad bean protein, dandelion, agrocybe aegerita, barberry, wild chrysanthemum flower, alternanthera philoxeroides and spirulina are combined, so that the immunity of the freshwater fish can be enhanced, and the survival rate of the grass carps is ensured without adding hormones.
It is to be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (7)

1. The feed additive for promoting the immunity of the grass carps to be improved is characterized by comprising the following components in parts by mass: DDGS5-10 parts, broad bean protein 5-10 parts, dandelion 0.15-0.35 part, garlic and ginger powder 0.05-0.25 part, agrocybe cylindracea 0.01-0.05 part, berberis 0.01-0.05 part, alpha-amylase 0.01-0.05 part, wild chrysanthemum flower 0.05-0.15 part, alternanthera philoxeroides 2-5 parts and spirulina 1-3 parts.
2. The feed additive for promoting grass carp immunity improvement according to claim 1, wherein the feed additive comprises: the feed additive for promoting the immunity of the grass carp to be improved is prepared from the following components in parts by weight: DDGS5 parts, broad bean protein 5 parts, dandelion 0.15 parts, garlic and ginger powder 0.05 parts, tea mushroom 0.01 parts, barberry 0.01 parts, alpha-amylase 0.01 parts, wild chrysanthemum flower 0.05 parts, water peanut 2 parts and spirulina 1 part.
3. The feed additive for promoting grass carp immunity improvement according to claim 1, wherein the feed additive comprises: the feed additive for promoting the immunity of the grass carp to be improved is prepared from the following components in parts by weight: DDGS10 parts, broad bean protein 10 parts, dandelion 0.35 parts, garlic and ginger powder 0.25 parts, agrocybe aegerita 0.05 parts, barberry 0.05 parts, alpha-amylase 0.05 parts, wild chrysanthemum flower 0.15 parts, alternanthera philoxeroides 5 parts and spirulina 3 parts.
4. The feed additive for promoting grass carp immunity improvement according to claim 1, wherein the feed additive comprises: the feed additive for promoting the immunity of the grass carp to be improved is prepared from the following components in parts by weight: DDGS6 parts, broad bean protein 8 parts, dandelion 0.2 parts, garlic and ginger powder 0.15 parts, tea mushroom 0.02 parts, barberry 0.04 parts, alpha-amylase 0.03 parts, wild chrysanthemum flower 0.1 parts, alternanthera philoxeroides 4 parts and spirulina 2 parts.
5. The use of the feed additive according to claims 1-4 in grass carp feed, wherein the feed additive is added into conventional grass carp feed and then processed into commercial feed. .
6. The feed additive for promoting immunity of grass carp and the application thereof according to claim 5, wherein the feed additive comprises: the doping amount of the feed additive is 2% -6% of the grass carp feed.
7. The feed additive for promoting immunity of grass carp and the application thereof according to claim 5, wherein the feed additive comprises: the doping amount of the feed additive is 3% of that of the grass carp feed.
CN202210739309.7A 2022-06-28 2022-06-28 Feed additive for promoting immunity improvement of grass carp and application thereof Pending CN115024441A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110012987A (en) * 2019-04-30 2019-07-16 盐城工学院 It is a kind of enhance fish immunity power feed addictive and its application
CN112244177A (en) * 2020-08-28 2021-01-22 盐城工学院 Compound Chinese herbal medicine feed additive for enhancing immunity of pikes and application thereof
CN113907216A (en) * 2021-09-01 2022-01-11 盐城工学院 Chinese herbal medicine feed additive for preventing and treating liver and gall syndrome of grass carp

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110012987A (en) * 2019-04-30 2019-07-16 盐城工学院 It is a kind of enhance fish immunity power feed addictive and its application
CN112244177A (en) * 2020-08-28 2021-01-22 盐城工学院 Compound Chinese herbal medicine feed additive for enhancing immunity of pikes and application thereof
CN113907216A (en) * 2021-09-01 2022-01-11 盐城工学院 Chinese herbal medicine feed additive for preventing and treating liver and gall syndrome of grass carp

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