CN112244177A - Compound Chinese herbal medicine feed additive for enhancing immunity of pikes and application thereof - Google Patents
Compound Chinese herbal medicine feed additive for enhancing immunity of pikes and application thereof Download PDFInfo
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- CN112244177A CN112244177A CN202010883858.2A CN202010883858A CN112244177A CN 112244177 A CN112244177 A CN 112244177A CN 202010883858 A CN202010883858 A CN 202010883858A CN 112244177 A CN112244177 A CN 112244177A
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- 239000003674 animal food additive Substances 0.000 title claims abstract description 35
- 230000036039 immunity Effects 0.000 title claims abstract description 29
- 150000001875 compounds Chemical class 0.000 title claims abstract description 15
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 15
- 241000411851 herbal medicine Species 0.000 title claims abstract description 15
- 241000277301 Esociformes Species 0.000 title abstract description 4
- 241000656145 Thyrsites atun Species 0.000 claims abstract description 37
- 240000002234 Allium sativum Species 0.000 claims abstract description 19
- 235000006886 Zingiber officinale Nutrition 0.000 claims abstract description 19
- 108010019077 beta-Amylase Proteins 0.000 claims abstract description 19
- 235000004611 garlic Nutrition 0.000 claims abstract description 19
- 235000008397 ginger Nutrition 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 19
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 18
- 240000002900 Arthrospira platensis Species 0.000 claims abstract description 18
- 235000016425 Arthrospira platensis Nutrition 0.000 claims abstract description 18
- 235000007340 Hordeum vulgare Nutrition 0.000 claims abstract description 18
- 240000000249 Morus alba Species 0.000 claims abstract description 18
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 18
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims abstract description 18
- 108010084695 Pea Proteins Proteins 0.000 claims abstract description 18
- 240000004534 Scutellaria baicalensis Species 0.000 claims abstract description 18
- 235000017089 Scutellaria baicalensis Nutrition 0.000 claims abstract description 18
- 235000019702 pea protein Nutrition 0.000 claims abstract description 18
- 229940082787 spirulina Drugs 0.000 claims abstract description 18
- 241000208688 Eucommia Species 0.000 claims abstract description 16
- 230000001737 promoting effect Effects 0.000 claims abstract description 12
- 230000006872 improvement Effects 0.000 claims abstract description 7
- 240000005979 Hordeum vulgare Species 0.000 claims abstract 6
- 241000234314 Zingiber Species 0.000 claims description 17
- 241000169203 Eichhornia Species 0.000 claims description 16
- 241000251468 Actinopterygii Species 0.000 abstract description 41
- 230000004083 survival effect Effects 0.000 abstract description 8
- 229940088597 hormone Drugs 0.000 abstract description 2
- 239000005556 hormone Substances 0.000 abstract description 2
- 240000003826 Eichhornia crassipes Species 0.000 abstract 2
- 244000273928 Zingiber officinale Species 0.000 abstract 2
- 230000009044 synergistic interaction Effects 0.000 abstract 1
- 235000019688 fish Nutrition 0.000 description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 22
- 238000002474 experimental method Methods 0.000 description 20
- 241001502129 Mullus Species 0.000 description 15
- 210000004369 blood Anatomy 0.000 description 15
- 239000008280 blood Substances 0.000 description 15
- 235000003642 hunger Nutrition 0.000 description 15
- 241000209219 Hordeum Species 0.000 description 12
- 210000002966 serum Anatomy 0.000 description 10
- 235000019750 Crude protein Nutrition 0.000 description 6
- 235000019784 crude fat Nutrition 0.000 description 6
- 102000004506 Blood Proteins Human genes 0.000 description 5
- 108010017384 Blood Proteins Proteins 0.000 description 5
- 102000016943 Muramidase Human genes 0.000 description 5
- 108010014251 Muramidase Proteins 0.000 description 5
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000001900 immune effect Effects 0.000 description 5
- 239000004325 lysozyme Substances 0.000 description 5
- 229960000274 lysozyme Drugs 0.000 description 5
- 235000010335 lysozyme Nutrition 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 210000003462 vein Anatomy 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
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- 241000238557 Decapoda Species 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 230000037351 starvation Effects 0.000 description 3
- 241000252210 Cyprinidae Species 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 244000237330 gutta percha tree Species 0.000 description 2
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- 230000004048 modification Effects 0.000 description 2
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- 239000000126 substance Substances 0.000 description 2
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- 241000252203 Clupea harengus Species 0.000 description 1
- 206010013654 Drug abuse Diseases 0.000 description 1
- 241000252232 Hypophthalmichthys Species 0.000 description 1
- 241000252234 Hypophthalmichthys nobilis Species 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000019514 herring Nutrition 0.000 description 1
- 230000000091 immunopotentiator Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 208000011117 substance-related disease Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Mycology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a compound Chinese herbal medicine feed additive for enhancing the immunity of pikes and application thereof, belonging to the technical field of fish feed. The feed additive for promoting the improvement of the immunity of the pike is prepared from the following components in parts by weight: 5-10 parts of barley malt, 5-10 parts of pea protein, 0.15-0.35 part of eucommia bark, 0.05-0.25 part of garlic and ginger powder, 0.01-0.05 part of mushroom, 0.01-0.05 part of scutellaria baicalensis, 0.01-0.05 part of beta-amylase, 0.05-0.15 part of mulberry leaf, 2-5 parts of water hyacinth and 1-3 parts of spirulina. The feed additive for promoting the immunity of the pike is combined with barley malt, pea protein, eucommia bark, mushroom, scutellaria baicalensis, mulberry leaves, water hyacinth and spirulina by utilizing the synergistic interaction of the garlic and ginger powder and the beta-amylase, so that the immunity of the pike can be enhanced, and the survival rate of the pike is ensured without adding hormone.
Description
Technical Field
The invention belongs to the technical field of fish feed, and particularly relates to a compound Chinese herbal medicine feed additive for enhancing the immunity of pikes and application thereof.
Background
And (3) pike: ctenophagogon idellus is a fish of Cyprinaceae and Clinopodium, is an important freshwater aquaculture fish in China, and forms four Chinese carps together with silver carps, bighead carps and herring. The pike has a history of more than 1700 years of cultivation, but the pike is eaten or sold after natural fry in rivers are cultivated in a pond, and the full artificial cultivation is really realized from the artificial propagation of four Chinese carps in 1958.
With the development of pollution-free sustainable culture and green, efficient and pollution-free fishery medicine, the research on green ecological, residual pollution-free and pollution-free immunopotentiators becomes the research focus of the aquaculture industry at present. The phenomenon of drug abuse in the existing culture process is serious, and the abuse and the residue of chemical drugs such as antibiotics and the like can not only endanger the culture of the shrimps and crabs, but also endanger the whole shrimp and crab culture industry.
Disclosure of Invention
The technical problem to be solved is as follows: aiming at the technical problem that chemical drugs such as antibiotics are abused, the invention provides the compound Chinese herbal medicine feed additive for enhancing the immunity of the pike and the application thereof, and the compound Chinese herbal medicine feed additive has the advantages of enhancing the immunity of the pike, having high survival rate, increasing the body mass of the fish fast and the like.
The technical scheme is as follows: the compound Chinese herbal medicine feed additive for enhancing the immunity of the pike comprises the following components in parts by mass: 5-10 parts of barley malt, 5-10 parts of pea protein, 0.15-0.35 part of eucommia bark, 0.05-0.25 part of garlic and ginger powder, 0.01-0.05 part of mushroom, 0.01-0.05 part of scutellaria baicalensis, 0.01-0.05 part of beta-amylase, 0.05-0.15 part of mulberry leaf, 2-5 parts of water hyacinth and 1-3 parts of spirulina.
Preferably, the feed additive for promoting the improvement of the immunity of the pike is prepared from the following components in parts by weight: 5 parts of barley malt, 5 parts of pea protein, 0.15 part of eucommia bark, 0.05 part of garlic and ginger powder, 0.01 part of mushroom, 0.01 part of scutellaria baicalensis, 0.01 part of beta-amylase, 0.05 part of mulberry leaf, 2 parts of water hyacinth and 1 part of spirulina.
Preferably, the feed additive for promoting the improvement of the immunity of the pike is prepared from the following components in parts by weight: 10 parts of barley malt, 10 parts of pea protein, 0.35 part of eucommia bark, 0.25 part of garlic and ginger powder, 0.05 part of mushroom, 0.05 part of scutellaria baicalensis, 0.05 part of beta-amylase, 0.15 part of mulberry leaf, 5 parts of water hyacinth and 3 parts of spirulina.
Preferably, the feed additive for promoting the improvement of the immunity of the pike is prepared from the following components in parts by weight: 6 parts of barley malt, 8 parts of pea protein, 0.2 part of eucommia bark, 0.15 part of garlic and ginger powder, 0.02 part of mushroom, 0.04 part of scutellaria baicalensis, 0.03 part of beta-amylase, 0.1 part of mulberry leaf, 4 parts of water hyacinth and 2 parts of spirulina.
Preferably, the doping amount of the feed additive is 2% -6% of the feed of the mullets.
Preferably, the doping amount of the feed additive is 3% of the feed of the mullets.
Has the advantages that: according to the invention, barley malt, pea protein, eucommia ulmoides, garlic and ginger powder, mushroom, scutellaria baicalensis, beta-amylase, mulberry leaves, water hyacinth and spirulina are adopted, and the synergistic effect of the garlic and ginger powder and the beta-amylase is utilized to be combined with the barley malt, the pea protein, the eucommia ulmoides, the mushroom, the scutellaria baicalensis, the mulberry leaves, the water hyacinth and the spirulina, so that the immunity of the freshwater fish can be enhanced, and the survival rate of the mullet is ensured without adding hormone.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The feed additive for promoting the immunity of the pike to be improved comprises the following components in parts by mass: 5 parts of barley malt, 5 parts of pea protein, 0.15 part of eucommia bark, 0.05 part of garlic and ginger powder, 0.01 part of mushroom, 0.01 part of scutellaria baicalensis, 0.01 part of beta-amylase, 0.05 part of mulberry leaf, 2 parts of water hyacinth and 1 part of spirulina.
The above raw material products are all selected from the commercial products. Wherein, the eucommia bark, the mushroom, the scutellaria baicalensis, the mulberry leaf, the water hyacinth and the spirulina are cleaned by deionized water and dried in the shade, then ground into 80-100 meshes, then evenly mixed with the barley malt, the pea protein, the garlic ginger powder and the beta-amylase, and then doped according to 5 percent of the conventional feed of the pike feed, and processed into the commercial feed according to the conventional processing mode of the pike feed.
Experiments were conducted in the Jiangsu salt City Tongwei (Dafeng) fodder Co. The experimental mullets were domesticated in the pond for 2 weeks, during which commercial mullet juvenile feed (28% crude protein, 4% crude fat) was fed. After 24h of hunger treatment, selecting individuals with strong physique and similar physique and weighing, setting 3 times of hunger treatment, randomly distributing the hunger treated individuals into 800L water tanks, and stocking 20 tails of experimental fishes with initial body mass (53.22 +/-0.14) g in each water tank. The feed additive and commercial young mullet feed adulterants (8:30 and 17:00) in the embodiment are fed for 2 times every day, and natural illumination is adopted during the period, and the air inflation is ceaselessly carried out. The culture experiment lasts for 60 days. At the beginning of the culture experiment, 5 fish were randomly picked and stored at-80 ℃ for analysis of the body composition of the initial sample. At the end of the culture experiment, the experimental fish are fasted for 24h, and the fish tails and the total mass of each water tank are counted. Then, 1mL of blood is randomly taken from the vein of 5 experimental fishes, the blood is kept still for 4 hours at the temperature of 4 ℃, and then the blood is centrifuged (800 Xg, 10min, 4 ℃), and serum is taken for measuring the immunological index. Through determination, the survival rate of the experimental fish is 100%, the mass of the terminal body is 130 +/-0.18 g, the activity of the serum lysozyme is 8.63 +/-0.92 unit/mu L, and the content of the serum protein is 51.40 +/-1.29 mg/mL.
Example 2
The feed additive for promoting the immunity of the pike is prepared from the following components in parts by weight: 10 parts of barley malt, 10 parts of pea protein, 0.35 part of eucommia bark, 0.25 part of garlic and ginger powder, 0.05 part of mushroom, 0.05 part of scutellaria baicalensis, 0.05 part of beta-amylase, 0.15 part of mulberry leaf, 5 parts of water hyacinth and 3 parts of spirulina.
The above raw material products are all selected from the commercial products. Wherein, the eucommia bark, the mushroom, the scutellaria baicalensis, the mulberry leaf, the water hyacinth and the spirulina are cleaned by deionized water and dried in the shade, then ground into 80-100 meshes, then evenly mixed with the barley malt, the pea protein, the garlic ginger powder and the beta-amylase, and then doped according to 4 percent of the conventional feed of the pike feed, and processed into the commercial feed according to the conventional processing mode of the pike feed.
Experiments were conducted in the Jiangsu salt City Tongwei (Dafeng) fodder Co. The experimental fish were kept in a water tank for 2 weeks while commercial young mullet feed (crude protein content 28%, crude fat content 4%) was fed. After 24h of hunger treatment, selecting individuals with strong physique and similar physique and weighing, setting 3 times of hunger treatment, randomly distributing the hunger treatment to 800L water tanks, and stocking 20 tails of experimental fishes with initial body mass (54.11 +/-0.22) g in each water tank. The feed additive and commercial young mullet feed adulterants (8:30 and 17:00) in the embodiment are fed for 2 times every day, and natural illumination is adopted during the period, and the air inflation is ceaselessly carried out. The culture experiment lasts for 60 days. At the beginning of the culture experiment, 5 fish were randomly picked and stored at-80 ℃ for analysis of the body composition of the initial sample. At the end of the culture experiment, the experimental fish are fasted for 24h, and the fish tails and the total mass of each water tank are counted. Then, 1mL of blood is randomly taken from the vein of 5 experimental fishes, the blood is kept still for 4 hours at the temperature of 4 ℃, and then the blood is centrifuged (800 Xg, 10min, 4 ℃), and serum is taken for measuring the immunological index. Through determination, the survival rate of the experimental fish is 100%, the mass of the final body is 134.22 +/-0.25 g, the activity of the serum lysozyme is 8.69 +/-0.71 unit/mu L, and the content of the serum protein is 51.86 +/-1.62 mg/mL.
Example 3
The feed additive for promoting the immunity of the pike to be improved comprises the following components in parts by mass: 6 parts of barley malt, 8 parts of pea protein, 0.2 part of eucommia bark, 0.15 part of garlic and ginger powder, 0.02 part of mushroom, 0.04 part of scutellaria baicalensis, 0.03 part of beta-amylase, 0.1 part of mulberry leaf, 4 parts of water hyacinth and 2 parts of spirulina.
The above raw material products are all selected from the commercial products. Wherein, the eucommia bark, the mushroom, the scutellaria baicalensis, the mulberry leaf, the water hyacinth and the spirulina are cleaned by deionized water and dried in the shade, then ground into 80-100 meshes, then evenly mixed with the barley malt, the pea protein, the garlic ginger powder and the beta-amylase, and then doped according to 3 percent of the conventional feed of the pike feed, and processed into the commercial feed according to the conventional processing mode of the pike feed.
Experiments were conducted in the Jiangsu salt City Tongwei (Dafeng) fodder Co. The experimental fish were kept in a water tank for 2 weeks while commercial young mullet feed (crude protein content 28%, crude fat content 4%) was fed. After the domesticated experimental fish is subjected to starvation treatment for 24 hours, selecting individuals with strong physique and similar physique and weighing, setting 3 times of the starvation treatment, randomly distributing the starvation treatment to 800L water tanks, and stocking 20 experimental fish tails with initial body mass (58.33 +/-0.14) g in each water tank. The feed additive and commercial young mullet feed adulterants (8:30 and 17:00) in the embodiment are fed for 2 times every day, and natural illumination is adopted during the period, and the air inflation is ceaselessly carried out. The culture experiment lasts for 60 days. At the beginning of the culture experiment, 5 fish were randomly picked and stored at-80 ℃ for analysis of the body composition of the initial sample. At the end of the culture experiment, the experimental fish are fasted for 24h, and the fish tails and the total mass of each water tank are counted. Then, 1mL of blood is randomly taken from the vein of 5 experimental fishes, the blood is kept still for 4 hours at the temperature of 4 ℃, and then the blood is centrifuged (800 Xg, 10min, 4 ℃), and serum is taken for measuring the immunological index. Through determination, the survival rate of the experimental fish is 100%, the mass of the terminal body is 145 +/-0.41 g, the activity of the serum lysozyme is 8.96 +/-0.51 unit/mu L, and the content of the serum protein is 55.12 +/-1.16 mg/mL.
Comparative example 1
The pure commercial young mullet feed (with 28% crude protein and 4% crude fat) was used.
Experiments were conducted in the Jiangsu salt City Tongwei (Dafeng) fodder Co. The experimental fish were kept in a water tank for 2 weeks while commercial young mullet feed (crude protein content 28%, crude fat content 4%) was fed. After 24h of hunger treatment, selecting individuals with strong physique and similar physique and weighing, setting 3 times of hunger treatment, randomly distributing the hunger treatment to 800L water tanks, and stocking 20 tails of experimental fishes with initial body mass of (57.11 +/-0.18) g in each water tank. Commercial juvenile mullet feed (8:00 and 16:00) in this example was fed 2 times per day apparently after being fed with food, and during this period, natural light was used, and aeration was continued. The culture experiment lasts for 60 days. At the beginning of the culture experiment, 5 fish were randomly picked and stored at-80 ℃ for analysis of the body composition of the initial sample. At the end of the culture experiment, the experimental fish are fasted for 24h, and the fish tails and the total mass of each water tank are counted. Then, 1mL of blood is randomly taken from the vein of 5 experimental fishes, the blood is kept still for 4 hours at the temperature of 4 ℃, and then the blood is centrifuged (800 Xg, 10min, 4 ℃), and serum is taken for measuring the immunological index. Through determination, the survival rate of the experimental fish is 100%, the mass of the final body is 116.87 +/-0.21 g, the activity of the serum lysozyme is 5.21 +/-0.18 unit/mu L, and the content of the serum protein is 40.35 +/-0.21 mg/mL.
Comparative example 2
The difference from example 3 is that no garlic ginger powder and no beta-amylase are added.
Experiments were conducted in the Jiangsu salt City Tongwei (Dafeng) fodder Co. The experimental fish were kept in a water tank for 2 weeks while commercial young mullet feed (crude protein content 28%, crude fat content 4%) was fed. After 24h of hunger treatment, selecting individuals with strong physique and similar physique and weighing, setting 3 times of hunger treatment, randomly distributing the hunger treatment to 800L water tanks, and stocking 20 tails of experimental fishes with initial body mass (57.15 +/-0.19) g in each water tank. The feed additive and commercial young mullet feed adulterants (8:00 and 16:00) in the embodiment are fed for 2 times every day, and natural illumination is adopted during the feeding period, and the air inflation is ceaselessly carried out. The culture experiment lasts for 60 days. At the beginning of the culture experiment, 5 fish were randomly picked and stored at-80 ℃ for analysis of the body composition of the initial sample. At the end of the culture experiment, the experimental fish are fasted for 24h, and the fish tails and the total mass of each water tank are counted. Then, 1mL of blood is randomly taken from the vein of 5 experimental fishes, the blood is kept still for 4 hours at the temperature of 4 ℃, and then the blood is centrifuged (800 Xg, 10min, 4 ℃), and serum is taken for measuring the immunological index. Through determination, the survival rate of the experimental fish is 100%, the mass of the final body is 124.11 +/-0.39 g, the activity of the serum lysozyme is 5.12 +/-0.21 unit/mu L, and the content of the serum protein is 42.12 +/-0.62 mg/mL.
Although the embodiments of the present invention have been described, various modifications and changes may be made by those skilled in the art without departing from the spirit and scope of the present invention, and such modifications and changes fall within the scope defined by the appended claims.
Claims (7)
1. The compound Chinese herbal medicine feed additive for enhancing the immunity of the pike is characterized in that the feed additive for promoting the immunity of the pike is prepared from the following components in parts by weight: 5-10 parts of barley malt, 5-10 parts of pea protein, 0.15-0.35 part of eucommia bark, 0.05-0.25 part of garlic and ginger powder, 0.01-0.05 part of mushroom, 0.01-0.05 part of scutellaria baicalensis, 0.01-0.05 part of beta-amylase, 0.05-0.15 part of mulberry leaf, 2-5 parts of water hyacinth and 1-3 parts of spirulina.
2. The compound Chinese herbal medicine feed additive for enhancing the immunity of the pike according to claim 1, wherein the feed additive for promoting the improvement of the immunity of the pike is prepared from the following components in parts by weight: 5 parts of barley malt, 5 parts of pea protein, 0.15 part of eucommia bark, 0.05 part of garlic and ginger powder, 0.01 part of mushroom, 0.01 part of scutellaria baicalensis, 0.01 part of beta-amylase, 0.05 part of mulberry leaf, 2 parts of water hyacinth and 1 part of spirulina.
3. The compound Chinese herbal medicine feed additive for enhancing the immunity of the pike according to claim 1, wherein the feed additive for promoting the improvement of the immunity of the pike is prepared from the following components in parts by weight: 10 parts of barley malt, 10 parts of pea protein, 0.35 part of eucommia bark, 0.25 part of garlic and ginger powder, 0.05 part of mushroom, 0.05 part of scutellaria baicalensis, 0.05 part of beta-amylase, 0.15 part of mulberry leaf, 5 parts of water hyacinth and 3 parts of spirulina.
4. The compound Chinese herbal medicine feed additive for enhancing the immunity of the pike according to claim 1, wherein the feed additive for promoting the improvement of the immunity of the pike is prepared from the following components in parts by weight: 6 parts of barley malt, 8 parts of pea protein, 0.2 part of eucommia bark, 0.15 part of garlic and ginger powder, 0.02 part of mushroom, 0.04 part of scutellaria baicalensis, 0.03 part of beta-amylase, 0.1 part of mulberry leaf, 4 parts of water hyacinth and 2 parts of spirulina.
5. The application of the compound Chinese herbal medicine feed additive for enhancing the immunity of the pike is characterized in that the compound Chinese herbal medicine feed additive for enhancing the immunity of the pike is added into the conventional pike feed and then processed into commercial feed.
6. The application of the compound Chinese herbal medicine feed additive for enhancing the immunity of the pike as claimed in claim 5, wherein the doping amount of the feed additive is 2% -6% of that of the pike feed.
7. The use of the compound Chinese herbal medicine feed additive for enhancing the immunity of the pike as claimed in claim 5, wherein the doping amount of the feed additive is 3% of that of the pike feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010883858.2A CN112244177A (en) | 2020-08-28 | 2020-08-28 | Compound Chinese herbal medicine feed additive for enhancing immunity of pikes and application thereof |
Applications Claiming Priority (1)
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| CN202010883858.2A CN112244177A (en) | 2020-08-28 | 2020-08-28 | Compound Chinese herbal medicine feed additive for enhancing immunity of pikes and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115176940A (en) * | 2022-07-26 | 2022-10-14 | 通威（大丰）饲料有限公司 | Compound Chinese herbal medicine feed additive for enhancing immunity of grass carp and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931939A (en) * | 2014-04-16 | 2014-07-23 | 盐城工学院 | Feed additive for controlling hepatobiliary syndromes of Liza haematocheila |
| CN105104893A (en) * | 2015-09-28 | 2015-12-02 | 盐城工学院 | Special-purpose puffing compound feed for mugil soiuy |
| CN110012987A (en) * | 2019-04-30 | 2019-07-16 | 盐城工学院 | It is a kind of enhance fish immunity power feed addictive and its application |
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2020
- 2020-08-28 CN CN202010883858.2A patent/CN112244177A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931939A (en) * | 2014-04-16 | 2014-07-23 | 盐城工学院 | Feed additive for controlling hepatobiliary syndromes of Liza haematocheila |
| CN105104893A (en) * | 2015-09-28 | 2015-12-02 | 盐城工学院 | Special-purpose puffing compound feed for mugil soiuy |
| CN110012987A (en) * | 2019-04-30 | 2019-07-16 | 盐城工学院 | It is a kind of enhance fish immunity power feed addictive and its application |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115176940A (en) * | 2022-07-26 | 2022-10-14 | 通威（大丰）饲料有限公司 | Compound Chinese herbal medicine feed additive for enhancing immunity of grass carp and application thereof |
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Application publication date: 20210122 |