CN115006480B - Traditional Chinese medicine composition for treating osteoporosis and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating osteoporosis and preparation method thereof Download PDFInfo
- Publication number
- CN115006480B CN115006480B CN202210408947.0A CN202210408947A CN115006480B CN 115006480 B CN115006480 B CN 115006480B CN 202210408947 A CN202210408947 A CN 202210408947A CN 115006480 B CN115006480 B CN 115006480B
- Authority
- CN
- China
- Prior art keywords
- parts
- chinese medicine
- traditional chinese
- deficiency
- treating osteoporosis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/02—Medicinal preparations containing materials or reaction products thereof with undetermined constitution from inanimate materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/24—Mucus; Mucous glands; Bursa; Synovial fluid; Arthral fluid; Excreta; Spinal fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/11—Pteridophyta or Filicophyta (ferns)
- A61K36/12—Filicopsida or Pteridopsida
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/11—Pteridophyta or Filicophyta (ferns)
- A61K36/12—Filicopsida or Pteridopsida
- A61K36/126—Drynaria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/236—Ligusticum (licorice-root)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/237—Notopterygium
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/238—Saposhnikovia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/284—Atractylodes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/286—Carthamus (distaff thistle)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/32—Burseraceae (Frankincense family)
- A61K36/328—Commiphora, e.g. mecca myrrh or balm of Gilead
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/533—Leonurus (motherwort)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/89—Cyperaceae (Sedge family)
- A61K36/8905—Cyperus (flatsedge)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
- A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The application relates to a traditional Chinese medicine composition for treating osteoporosis, which is prepared from the following traditional Chinese medicine raw materials: astragalus root, pilose asiabell root, largehead atractylodes rhizome, indian buead, safflower, szechuan lovage rhizome, motherwort herb, earthworm, tangerine peel, incised notopterygium rhizome, divaricate saposhnikovia root, myrrh, trogopterus dung, nutgrass galingale rhizome, eucommia bark, chinese taxillus twig, east Asian tree fern rhizome, drynaria rhizome, oyster shell and dragon bone.
Description
Technical Field
The application belongs to the field of traditional Chinese medicine preparations, and in particular relates to a traditional Chinese medicine composition for treating osteoporosis and a preparation method thereof.
Background
Osteoporosis is a systemic bone disease that is prone to fracture due to a decrease in bone density and bone mass caused by various causes, destruction of bone microstructure, and an increase in bone fragility. Osteoporosis often occurs in postmenopausal and elderly people and is caused by local or systemic degenerative changes in the bone joints due to senile physical weakness, loss of bone nourishment, loss of qi and blood, and the like. Su Wen-flaccid theory, day: kidney qi heat causes the waist and spine to be unstable, and the bone to be dry and marrow to be reduced, which is called flaccidity. "yet day: the kidney is also the reservoir of water, so that the water does not over fire, but the bone is dry and marrow is deficient, so that the foot is not in any condition and flaccidity of bone occurs. "the blood circulation depends on the promotion of primordial qi, which is the transformation of kidney essence, kidney essence deficiency, passive qi transformation, blood stasis, namely kidney deficiency and blood stasis; spleen deficiency is a source of qi deficiency due to the biochemical failure of qi, and qi deficiency is insufficient to promote blood circulation, so that blood stasis is necessary. As pointed out in Wangqing Jilin-adapted Imperial: the primordial qi is deficient and must not reach the blood vessels, so that the blood vessels have no qi and must stay for blood stasis. "kidney deficiency is the main mechanism leading to aging, and blood stasis accelerates this process. Since osteoporosis is a progressive disease, the osteoporosis is easily ignored in the early stage, and over time, the osteoporosis becomes more serious and presents symptoms of qi deficiency and blood stasis, and a medicament for treating the qi deficiency and blood stasis type osteoporosis, which treats both symptoms and root causes, is lacking at present.
Disclosure of Invention
In view of the defects of the prior art, the application aims to provide a traditional Chinese medicine composition for treating osteoporosis with the effects of treating both symptoms and root causes and qi deficiency and blood stasis.
Another object of the application is a process for the preparation and use of the application.
The purpose of the application is realized in the following way:
a traditional Chinese medicine composition for treating osteoporosis is prepared from the following traditional Chinese medicine raw materials in parts by weight: 20-30 parts of astragalus membranaceus, 15-25 parts of codonopsis pilosula, 20-30 parts of bighead atractylodes rhizome, 8-12 parts of poria cocos, 6-10 parts of safflower, 15-25 parts of ligusticum wallichii, 10-16 parts of motherwort herb, 8-12 parts of earthworm, 18-22 parts of dried orange peel, 12-16 parts of notopterygium root, 8-12 parts of radix sileris, 8-12 parts of myrrh, 10-16 parts of trogopterus dung, 3-6 parts of nutgrass galingale rhizome, 12-16 parts of eucommia bark, 10-16 parts of mistletoe, 18-22 parts of rhizoma cibotii, 10-14 parts of rhizoma drynariae, 10-16 parts of oyster and 12-16 parts of dragon bone.
The traditional Chinese medicine composition for treating osteoporosis is prepared from the following traditional Chinese medicine raw materials in parts by weight: 25 parts of astragalus, 20 parts of codonopsis pilosula, 25 parts of bighead atractylodes rhizome, 10 parts of poria cocos, 8 parts of safflower, 20 parts of ligusticum wallichii, 13 parts of motherwort herb, 10 parts of earthworm, 20 parts of dried orange peel, 14 parts of notopterygium root, 10 parts of radix sileris, 10 parts of myrrh, 13 parts of trogopterus dung, 5 parts of nutgrass galingale rhizome, 13 parts of eucommia bark, 13 parts of mistletoe, 20 parts of rhizoma cibotii, 12 parts of rhizoma drynariae, 14 parts of oyster and 14 parts of dragon bone.
The traditional Chinese medicine composition for treating osteoporosis is prepared from the following traditional Chinese medicine raw materials in parts by weight: 20 parts of astragalus, 25 parts of codonopsis pilosula, 20 parts of bighead atractylodes rhizome, 12 parts of poria cocos, 6 parts of safflower, 25 parts of ligusticum wallichii, 10 parts of motherwort, 12 parts of earthworm, 18 parts of dried orange peel, 16 parts of notopterygium root, 8 parts of radix sileris, 12 parts of myrrh, 10 parts of trogopterus dung, 6 parts of nutgrass galingale rhizome, 12 parts of eucommia bark, 16 parts of mistletoe, 18 parts of rhizoma cibotii, 14 parts of rhizoma drynariae, 10 parts of oyster and 16 parts of dragon bone.
The traditional Chinese medicine composition for treating osteoporosis is prepared from the following traditional Chinese medicine raw materials in parts by weight: 30 parts of astragalus, 15 parts of codonopsis pilosula, 30 parts of bighead atractylodes rhizome, 8 parts of poria cocos, 10 parts of safflower, 15 parts of ligusticum wallichii, 16 parts of motherwort, 8 parts of earthworm, 22 parts of dried orange peel, 12 parts of notopterygium root, 12 parts of radix sileris, 8 parts of myrrh, 16 parts of trogopterus dung, 3 parts of nutgrass galingale rhizome, 16 parts of eucommia bark, 10 parts of mistletoe, 22 parts of rhizoma cibotii, 10 parts of rhizoma drynariae, 16 parts of oyster and 12 parts of dragon bone.
The preparation method of the traditional Chinese medicine composition for treating osteoporosis comprises the following steps:
(1) Decocting Concha Ostreae and Os Draconis with 8 times of water for 1 hr;
(2) Extracting Carthami flos, rhizoma Ligustici Chuanxiong, pericarpium Citri Tangerinae, myrrha, oletum Trogopterori, and rhizoma Cyperi by steam distillation, and extracting volatile oil;
(3) Adding the residues and other medicinal materials obtained after the steam distillation extraction of the safflower, the ligusticum wallichii, the dried orange peel, the myrrh, the trogopterus dung and the rhizoma cyperi in the step (1) into the decoction of the oyster and the dragon bone in the step (2), adding the steam distillation decoction in the step (2), supplementing 10 times of total weight of water of all medicinal materials, decocting for three times, combining the water extracts each time for 2 hours, filtering to obtain a mixed water extract, heating and concentrating to obtain a mixed water extract concentrate, concentrating and drying under reduced pressure, and spraying the safflower, the ligusticum wallichii, the dried orange peel, the myrrh, the trogopterus dung and the rhizoma cyperi volatile oil obtained in the step (2);
(4) Adding adjuvants, and making into tablet, capsule, or granule.
The application of the traditional Chinese medicine composition for treating osteoporosis in preparing the medicine for treating osteoporosis.
The application of the traditional Chinese medicine composition for treating osteoporosis in preparation of the medicine for treating osteoporosis is that the osteoporosis is qi deficiency and blood stasis.
The application of the traditional Chinese medicine composition for treating osteoporosis in preparing the medicine for treating osteoporosis can improve the TGF-beta 1, BGP and ALP levels in serum.
The application of the traditional Chinese medicine composition for treating osteoporosis in preparing the medicine for treating osteoporosis can reduce the TRACP level in serum.
The application of the traditional Chinese medicine composition for treating osteoporosis in preparing the medicine for treating osteoporosis can improve the expression of TGF-beta 1 protein.
The radix astragali is root of Astragalus mongholicus bge of Leguminosae. Collected in spring and autumn, removed with soil, fibrous root and root head, sun dried to six seven times, bundled and dried. The main functions are as follows: tonify qi, strengthen superficies, expel pus, promote urination and promote tissue regeneration. Can be used for treating qi deficiency, debilitation, chronic diarrhea, rectocele, spontaneous perspiration, edema, uterine prolapse, chronic nephritis, albuminuria, diabetes, and chronic open sore.
The radix Codonopsis is dry root of radix Codonopsis, or radix Codonopsis Lanceolatae of Campanulaceae. Dang Shen is sweet in flavor and neutral in nature. Has effects in invigorating spleen, replenishing qi, quenching thirst, invigorating spleen, tonifying lung, nourishing blood, and promoting salivation. Can be used for treating spleen and lung qi deficiency, anorexia, cough, asthma, deficiency of qi and blood, sallow complexion, palpitation, short breath, thirst due to body fluid deficiency, and internal heat and diabetes. [1] Lazy speaking, weak limbs, poor appetite, qi deficiency, deficiency of both qi and fluid, deficiency of both qi and blood, and chlorosis due to blood deficiency. However, the exterior syndrome is not relieved but the excessive pathogenic factors in the middle-jiao cannot be used. The product has similar efficacy to that of Ginseng radix, but weak potency. For general deficiency syndrome, it can be used instead of Ginseng radix; for severe deficiency syndrome, it is usually combined with Shen. Collected in autumn, washed and dried in the sun.
The Atractylodis rhizoma is rhizome of Atractylodes macrocephala Atractylodes macrocephala Koidz. Warm nature, sweet and bitter. Enter spleen meridian and stomach meridian. Has effects in invigorating spleen, invigorating qi, eliminating dampness, promoting diuresis, relieving sweating, and preventing miscarriage. Belongs to qi-tonifying herbs in the category of deficiency-tonifying herbs. Has obvious and durable antidiuretic effect, and has obvious inhibiting effect on Ehrlich carcinoma of mice, ascites tumor of lymphosarcoma and esophageal carcinoma of human 109; has bidirectional regulating effect on gastrointestinal system, and can be used for treating gastric ulcer; in addition, it has the pharmacological actions of spasmolysis, protecting liver and resisting bacteria. It also has effects in promoting gastrointestinal peristalsis, promoting gallbladder function, resisting oxidation, reducing blood sugar, resisting blood coagulation, resisting bacteria, dilating blood vessel, inhibiting heart, and tranquilizing mind.
Poria is the dry sclerotium of Wolf, a fungus of the Polyporaceae family, poria cocos (Schw.) co. Sweet and light in taste and neutral in nature. It enters heart, lung, spleen and kidney meridians. Induce diuresis and excrete dampness, invigorate spleen and calm heart. Can be used for treating edema, oliguria, phlegm retention, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia.
Safflower is a dried flower of Compositae plant safflower Carthamus tinctorius L. The flowers are picked when the flowers turn from yellow to red, and dried in the shade or sun-dried. Cultivation is widely carried out in various places throughout the country. Has effects of promoting blood circulation, dredging channels, removing blood stasis, and relieving pain. Can be used for treating amenorrhea, dysmenorrhea, lochia, abdominal mass, chest pain, abdominal pain due to stagnation, chest and hypochondrium stabbing pain, traumatic injury, and pyocutaneous disease swelling and pain.
Ligusticum wallichii is a traditional Chinese medicine plant commonly used for promoting blood circulation and promoting qi circulation, dispelling wind and relieving pain, and being spicy, warm and fragrant, can travel without being blocked, can travel and can travel upwards to reach the peak; blood flows into the body and down to the sea. The effects of activating blood and removing stasis are wide, and the traditional Chinese medicine composition is suitable for various diseases of blood stasis retardation; can be used for treating headache due to wind-damp evil, etc. Sichuan lovage rhizome is a qi-flowing herb in blood, and has the functions of dispersing in the body, resolving depression, dredging the meridian passage, relieving pain, etc.
Herba Leonuri is fresh or dry aerial part of herba Leonuri Leonurus japonicus Houtt of Labiatae. Harvesting fresh spring seedling stage to early stage of early summer flower; the dry product is obtained by cutting and sun-drying the stem and leaf in summer, or cutting and sun-drying the stem and leaf in summer when the flower is not opened or is initially opened. Widely distributed throughout the country. Has effects of promoting blood circulation, regulating menstruation, inducing diuresis, relieving swelling, and clearing heat and toxic substances. Can be used for treating menoxenia, dysmenorrhea, amenorrhea, lochiorrhea, edema, oliguria, and pyocutaneous disease.
The Lumbricus has effects of clearing heat, arresting convulsion, dredging collaterals, relieving asthma, and promoting urination. It is used for treating hyperpyrexia, unconsciousness, convulsion, arthralgia, cough due to lung heat, oliguria, edema, and hypertension after parching. Mainly contains various amino acids.
The pericarpium Citri Tangerinae is dried mature pericarp of Citrus reticulata Blanco Citrus reticulata Blanco and its cultivar. The medicinal materials are divided into dried orange peel and dried orange peel. Picking ripe fruits, peeling peel, and sun drying or low temperature drying. Bitter and pungent in flavor and warm in nature. Enter lung and spleen meridians. Regulate qi, invigorate spleen, dry dampness and resolve phlegm. Can be used for treating abdominal distention, anorexia, vomiting and diarrhea, cough with excessive phlegm.
Notopterygium incisum is an umbrella-type plant of Notopterygium, perennial herb, 60-120 cm high, strong in rhizome, and bamboo-shaped in elongation. The root neck is provided with a wilt leaf sheath. The stem is upright, cylindrical, hollow, has vertical fine stripes and is purple. It has warm nature, and can be used for relieving exterior syndrome, dispelling cold, and eliminating cold dampness, and can be used for treating wind cold, headache, perspiration, arthralgia due to cold dampness, and rheumatalgia of upper limbs. Growing in the forest margin and the bush at the altitude of 2000-4000 meters; distributed in Shaanxi, sichuan, gansu, qinghai and Tibet of China.
Radix Saposhnikoviae is root of radix Saposhnikoviae belonging to Umbelliferae. Pungent and sweet in flavor and slightly warm in nature. It enters the urinary bladder, spleen and liver meridians. Dispel wind, release exterior, dispel dampness, alleviate pain and stop spasm. Is used for treating exterior syndrome, rubella pruritus, rheumatalgia, tetanus, spleen deficiency and excessive dampness.
Myrrh is a dry resin of the olivaceae plant land Ding Shu Commiphora myrrha engl or halibut Ding Shu Commiphora molmol engl. Is divided into natural myrrh and gum myrrh. Distributed in the south of Somali, egyptian, arabian peninsula, etc. Has the effects of removing blood stasis, relieving pain, relieving swelling and promoting granulation. It is used for treating chest pain, pain in stomach, dysmenorrhea, amenorrhea, puerperal stagnation, abdominal mass, abdominal pain, rheumatalgia, traumatic injury, carbuncle, swelling, and skin infection.
The Oletum Trogopterori is dry feces of peter mice and mice of the family Trogopterori. Can be collected all the year round, but the spring and autumn are more important, and the quality of the spring is better. After the extraction, the impurities such as sand, soil and the like are cleaned, and the impurities are respectively classified into two types of trogopterus dung blocks and trogopterus dung rice according to the shape. The main functions are as follows: unprocessed with blood circulation to stop pain. For pain due to blood and qi in heart and abdomen, amenorrhea, postpartum blood stasis; it is indicated for snake, scorpion and centipede bites. Stir-baked to stop bleeding. For woman metrorrhagia, excessive menstruation and leucorrhea with reddish discharge.
The rhizoma Cyperi is rhizome of Cyperaceae perennial herb Cyperus rotundus. Mainly produced in Zhejiang, hunan, guangdong, henan, sichuan, shandong and other places. Plucking in autumn, singeing, boiling in boiling water, or steaming, sun drying, or sun drying directly. Raw materials, stir-baked with vinegar or stir-baked into charcoal. Pungent, slightly bitter, slightly sweet, and flat. Enter liver and triple energizer meridians. Liver soothing, qi regulating, menstruation regulating and pain relieving. Can be used for treating hypochondriac pain, abdominal distention pain, hernia pain, menoxenia, and breast pain.
Eucommia ulmoides is bark of eucommia ulmoides Oliver belonging to family Eucommiaceae. To protect resources, a local peeling method is generally used. Selecting plants growing for more than 15-20 years from Qingming to summer, peeling off bark, peeling off coarse bark, and sun drying according to the specification and size of medicinal materials. And (5) placing the product at a ventilation drying place. The main functions are as follows: liver and kidney tonifying, tendons and bones strengthening, miscarriage preventing. For soreness and pain of waist and knees, weakness of feet and knees, dribbling urine, itching of yin, abortion, fetal movement, and hypertension.
The herba Taxilli is dry leaf and stem branch of herba Taxilli Taxillus chinensis (DC.) Danser. Harvesting in winter to next spring, removing coarse stems, cutting, drying, or steaming and drying. Bitter and sweet in flavor and neutral in nature. It enters liver and kidney meridians. Has effects of dispelling pathogenic wind and dampness, nourishing liver and kidney, strengthening tendons and bones, and tranquilizing. Can be used for treating rheumatalgia, soreness of waist and knees, weakness of tendons and bones, metrorrhagia, metrostaxis, blood leakage during pregnancy, fetal irritability, and dizziness.
The rhizoma Cibotii is dried rhizome of rhizoma Cibotii Cibotium barometz (L.) J.Sm. Digging in autumn and winter, removing silt, and drying; or removing hard root, petiole and golden yellow villus, slicing, drying to obtain rhizoma Cibotii tablet; steaming, sun drying to six or seven times, cutting into thick slices, and drying to obtain the final product. Bitter and sweet in flavor and warm in nature. It enters liver and kidney meridians. Dispelling wind-damp, tonifying liver and kidney, strengthening waist and knee. Can be used for treating rheumatalgia, soreness of waist and knees, and weakness of lower limbs.
Rhizoma Drynariae is rhizome of rhizoma Drynariae of Dryopteris Crassirhizomes of herba Emiliae or herba Emiliae of perennial plant of Polyphytic of Nymphaeaceae. Produced in the south and south China, the south and west China, zhejiang, fujian, taiwan, etc. Collecting at any time, removing leaf and silt, cleaning, slicing, and drying. Unprocessed or scalding with sand. It enters liver and kidney meridians. Tonify kidney, activate blood, stop bleeding, and strengthen tendons and bones. Can be used for treating lumbago due to kidney deficiency, tinnitus, deafness, traumatic injury, and swelling and pain.
Oyster is shell of oyster of Crassostreae, crassostrea gigas or crassostrea gigas. Can be collected all the year round. After obtaining, removing meat, taking out shell, cleaning and drying in the sun. The main functions are as follows: astringing yin, suppressing yang, suppressing sweating, astringing essence, eliminating phlegm, and softening hard masses. It is indicated for convulsive epilepsy, dizziness, spontaneous perspiration, night sweat, spermatorrhea, stranguria with turbid urine, metrorrhagia, leukorrhagia, scrofula and goiter. Can be used for treating palpitation, insomnia, dizziness, tinnitus, scrofula, phlegm nodule, spontaneous perspiration, night sweat, spermatorrhea, leukorrhagia, gastralgia, and pantothenic acid. Calcined oyster has the effect of astringing and inducing astringency. Can be used for treating spontaneous perspiration, night sweat, spermatorrhea, leukorrhagia, and gastralgia.
The Os Draconis is fossil of ancient large mammal animal such as animal, three-toe horse, rhinocerotis, deer, and cattle. Has effects of relieving convulsion, tranquilizing, suppressing hyperactive liver, subsiding yang, astringing, and inducing astringency. Is mainly used for treating restlessness of heart-mind, palpitation, insomnia, convulsive epilepsy, mania, dizziness due to liver-yang, and chronic ulcer due to the symptoms of damp sore, prurigo and skin. Astringent, sweet and neutral in nature. It enters heart, liver and kidney meridians. Calm heart and tranquilize mind, calm liver and subdue yang, induce astringency and astringe. Can be used for treating palpitation, insomnia, amnesia, convulsion, epilepsy, giddiness, spontaneous perspiration, night sweat, spermatorrhea, enuresis, metrorrhagia, leukorrhagia, chronic diarrhea, chronic dysentery, ulcer, and damp sore.
The beneficial effects are that: osteoporosis due to qi deficiency and blood stasis is frequently seen in middle and late stages of osteoporosis. Mainly due to the aging of organisms, kidney essence and qi deficiency, gradual consumption of primordial qi, weak blood circulation, slow blood circulation and stasis, clinically manifested as lumbago, soreness and weakness of waist and knees, flaccidity of lower limbs, blue and purple lips and tongue, and thready and astringent pulse. The application treats through five aspects, namely, due to insufficient qi transformation power of a body, spleen yang qi is consumed, phlegm dampness is heavy, yin is injured for a long time, qi transformation is unfavorable, and anorexia and abdominal distention are caused, so that qi is supplemented and spleen and stomach are regulated; secondly, tonifying qi and regulating spleen and stomach is easy to increase in-vivo moisture, and the qi is required to be regulated and dehumidified; thirdly, the blood circulation is slow and the blood stasis is needed to be activated; fourthly, because qi is the general of blood, blood is the mother of qi, blood is not generated alone, qi is relied on to generate, blood and qi are required to be moved, and pain is relieved; fifthly, osteoporosis due to qi deficiency and blood stasis occurs in the elderly and infirm patients, kidney is the congenital origin, the life root, kidney stores essence, main bone generates marrow, kidney excess causes bone to generate qi, and yang and liver and kidney are needed to be reinforced; the sixth herb is salty in nature and enters the kidney to strengthen the condyle, and the other herbs are led to enter the kidney meridian.
The application uses astragalus root, pilose asiabell root, largehead atractylodes rhizome and Indian buead for tonifying qi and regulating spleen and stomach, safflower, szechuan lovage rhizome, trogopterus dung and earthworm for activating blood and dissolving stasis, tangerine peel and notopterygium root for regulating qi and dehumidifying, and is matched with qi-tonifying and spleen-stomach-regulating medicines, myrrh, trogopterus dung and nutgrass galingale rhizome for promoting blood and qi, relieving pain and is matched with blood-activating and stasis-dissolving medicines, and all medicines are monarch medicines; eucommia ulmoides, chinese taxillus twig, rhizoma cibotii and rhizoma drynariae are used as ministerial drugs, oyster and fossil fragments are used as adjuvant drugs for tonifying kidney and strengthening bones, and the whole formula is used for treating qi deficiency and blood stasis type osteoporosis, so that the effect is remarkable. Clinical experiments show that the total effective rate of the application reaches 92 percent, which is higher than that of a control group; pharmacodynamic experiments also show that after patients take the application, the TGF-beta 1, BGP and ALP levels in serum can be increased, the TRACP level is reduced, and further research is related to up-regulating the expression of TGF-beta 1 protein.
Drawings
FIG. 1 is a graph showing the comparison of clinical effects of the application on osteoporosis patients with qi deficiency and blood stasis;
comparison of TGF-beta 1 protein expression levels in each group in FIG. 2.
Detailed Description
The above-described matters of the present application will be further described in detail by way of examples, but it should not be construed that the scope of the above-described subject matter of the present application is limited to the following examples, and all techniques realized based on the above-described matters of the present application are within the scope of the present application.
Example 1: taking 20g of astragalus, 25g of codonopsis pilosula, 20g of bighead atractylodes rhizome, 12g of poria cocos, 6g of safflower, 25g of ligusticum wallichii, 10g of motherwort, 12g of earthworm, 18g of dried orange peel, 16g of notopterygium root, 8g of radix sileris, 12g of myrrh, 10g of trogopterus dung, 6g of rhizoma cyperi, 12g of eucommia ulmoides, 16g of Chinese taxillus twig, 18g of rhizoma cibotii, 14g of rhizoma drynariae, 10g of oyster and 16g of dragon bone;
the preparation method comprises the following steps:
(1) Decocting Concha Ostreae and Os Draconis with 8 times of water for 1 hr;
(2) Extracting Carthami flos, rhizoma Ligustici Chuanxiong, pericarpium Citri Tangerinae, myrrha, oletum Trogopterori, and rhizoma Cyperi by steam distillation, and extracting volatile oil;
(3) Adding the residues and other medicinal materials obtained after the steam distillation extraction of the safflower, the ligusticum wallichii, the dried orange peel, the myrrh, the trogopterus dung and the rhizoma cyperi in the step (1) into the decoction of the oyster and the dragon bone in the step (2), adding the steam distillation decoction in the step (2), supplementing 10 times of total weight of water of all medicinal materials, decocting for three times, combining the water extracts each time for 2 hours, filtering to obtain a mixed water extract, heating and concentrating to obtain a mixed water extract concentrate, concentrating and drying under reduced pressure, and spraying the safflower, the ligusticum wallichii, the dried orange peel, the myrrh, the trogopterus dung and the rhizoma cyperi volatile oil obtained in the step (2);
(4) Adding starch, and making into tablet.
The embodiment 2 comprises 30g of astragalus, 15g of codonopsis pilosula, 30g of bighead atractylodes rhizome, 8g of poria cocos, 10g of safflower, 15g of ligusticum wallichii, 16g of motherwort, 8g of earthworm, 22g of dried orange peel, 12g of notopterygium root, 12g of radix sileris, 8g of myrrh, 16g of trogopterus dung, 3g of nutgrass galingale rhizome, 16g of eucommia bark, 10g of Chinese taxillus twig, 22g of rhizoma cibotii, 10g of rhizoma drynariae, 16g of oyster and 12g of dragon bone;
the preparation method comprises the following steps:
(1) Decocting Concha Ostreae and Os Draconis with 8 times of water for 1 hr;
(2) Extracting Carthami flos, rhizoma Ligustici Chuanxiong, pericarpium Citri Tangerinae, myrrha, oletum Trogopterori, and rhizoma Cyperi by steam distillation, and extracting volatile oil;
(3) Adding the residues and other medicinal materials obtained after the steam distillation extraction of the safflower, the ligusticum wallichii, the dried orange peel, the myrrh, the trogopterus dung and the rhizoma cyperi in the step (1) into the decoction of the oyster and the dragon bone in the step (2), adding the steam distillation decoction in the step (2), supplementing 10 times of total weight of water of all medicinal materials, decocting for three times, combining the water extracts each time for 2 hours, filtering to obtain a mixed water extract, heating and concentrating to obtain a mixed water extract concentrate, concentrating and drying under reduced pressure, and spraying the safflower, the ligusticum wallichii, the dried orange peel, the myrrh, the trogopterus dung and the rhizoma cyperi volatile oil obtained in the step (2);
(4) Adding starch, and making into capsule.
Example 3: taking 25g of astragalus, 20g of codonopsis pilosula, 25g of bighead atractylodes rhizome, 10g of poria cocos, 8g of safflower, 20g of ligusticum wallichii, 13g of motherwort, 10g of earthworm, 20g of dried orange peel, 14g of notopterygium root, 10g of radix sileris, 10g of myrrh, 13g of trogopterus dung, 5g of nutgrass galingale rhizome, 13g of eucommia bark, 13g of Chinese taxillus twig, 20g of rhizoma cibotii, 12g of rhizoma drynariae, 14g of oyster and 14g of dragon bone;
the preparation method comprises the following steps:
(1) Decocting Concha Ostreae and Os Draconis with 8 times of water for 1 hr;
(2) Extracting Carthami flos, rhizoma Ligustici Chuanxiong, pericarpium Citri Tangerinae, myrrha, oletum Trogopterori, and rhizoma Cyperi by steam distillation, and extracting volatile oil;
(3) Adding the residues and other medicinal materials obtained after the steam distillation extraction of the safflower, the ligusticum wallichii, the dried orange peel, the myrrh, the trogopterus dung and the rhizoma cyperi in the step (1) into the decoction of the oyster and the dragon bone in the step (2), adding the steam distillation decoction in the step (2), supplementing 10 times of total weight of water of all medicinal materials, decocting for three times, combining the water extracts each time for 2 hours, filtering to obtain a mixed water extract, heating and concentrating to obtain a mixed water extract concentrate, concentrating and drying under reduced pressure, and spraying the safflower, the ligusticum wallichii, the dried orange peel, the myrrh, the trogopterus dung and the rhizoma cyperi volatile oil obtained in the step (2);
(4) Adding dextrin, and making into granule.
Example 4: clinical trial
1. 100 cases of osteoporosis patients with qi deficiency and blood stasis, 50 cases for men and 50 cases for women, which are treated in China, are selected from 9 months in 2019 to 10 months in 2020; randomly dividing into a control group and an experimental group, wherein the ages are 59-84 years, and the average ages are 72.68); the course of the disease is 1-13 years, and the average course of the disease is 7.56 years. The clinical manifestations of traditional Chinese medicine include pain in the waist and back, soreness and weakness of the waist and knees, flaccidity of lower limbs, blue and purple lips and tongue, and thready and astringent pulse.
2. Inclusion criteria (1) inclusion criteria are in compliance with relevant diagnosis in the diagnosis and treatment guide for primary osteoporosis (2017); hormone medicines are not taken in a short period; the blood coagulation function is normal; no cognitive impairment; and signing the agreement. (2) exclusion criteria: severe organic diseases; liver insufficiency; mental disorders; osteoporosis due to other factors; intolerance to study drugs.
3. Observing the treatment effect of the index (1): the effect is shown: pain symptoms disappeared, bone density (BMD) and osteocalcin (BGP) improved significantly; the method is effective: the pain symptom is obviously relieved, and BMD and BGP are improved; invalidation: clinical symptoms did not disappear, pain did not subside, BMD and BGP did not improve, and even aggravated. The sum of obvious effect and effective effect is the total effect. (2) serological index: before and after treatment, 4ml of venous blood is extracted from the patient in early morning and after treatment, serum is centrifugally separated, and the serum conversion factor-beta 1 (TGF-beta 1), osteocalcin (BGP), bone alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRACP) levels of the patient are measured by adopting an enzyme-linked immunosorbent double antibody sandwich method. (3) security: two groups of nausea, vomiting, bone pain, joint pain and the like are counted.
4. The treatment method comprises the following steps: the granule prepared in the embodiment 3 of the application is taken by an experimental group, 3 times a day, two bags each time, and one month is a treatment course. Zoledronic acid (national standard of medicine: H20113138) was administered to the control group for treatment by intravenous drip at 15-20min,5mg/d, and mixed with 250ml of 0.9% sodium chloride solution for injection.
5. The results of the treatment effect comparison are shown in the following table and fig. 1.
| Example of obvious effect | Effective examples | Ineffective example | Total effective rate (%) | |
| Control group | 25 | 15 | 10 | 80 |
| Experimental group | 20 | 26 | 4 | 92 |
6. The two sets of serological indices are compared to the following table.
Comparison with Pre-treatment * p<0.05 ** p<0.01
Conclusion: serum transforming growth factor-beta 1 (TGF-beta 1) participates in various pathophysiological activities of organisms, and can mediate cartilage synthesis and inhibit collagen glycan decomposition; osteocalcin (BGP) is secreted by osteoblasts, and reflects the activity state of newly formed bone cells and regulates bone metabolism; bone alkaline phosphatase (ALP) is an osteoblast phenotype marker used for reflecting the activity and function of bone cells and evaluating the bone mineralization disorder of human body; tartrate-resistant acid phosphatase (TRACP) reflects bone cell resorption and osteoclast activity. The result of the application shows that compared with the control group, the total effective rate of the treatment of the observation group is higher; after treatment, TGF-beta 1, BGP and ALP levels were higher than that of the control group, and TRACP levels were lower than that of the control group. Therefore, the application has good effect for treating osteoporosis. The medicine is clinically used for osteoporosis with definite curative effect and less adverse reaction.
Example 5: pharmacodynamic study
1. Modeling and grouping
Healthy female SD rats were fed with rat-specific feed for 1 week and kept in indoor animal houses maintained at 22-26℃under the same lighting conditions and humidity conditions. After 1 week of adaptive feeding, 40 SD rats are taken and grouped according to a random number table method, and the SD rats are divided into 4 groups according to an experimental plan, namely a normal control group, a model group and a high-low dose group. The experiment uses Hulley method to build GIOP rat model. The method is that the rats in the model group and the high and low dose group of the application are injected subcutaneously with methylprednisolone (5.0 mg/kg,1 time/d). After successful molding, the high and low dose group rats of the application are irrigated with the granules of the embodiment 3, the concentration is respectively 20g crude drug/kg and 5g crude drug/kg, and the stomach is irrigated according to 10mL/kg for 6 weeks once a day; the model group and the normal control group were perfused with 0.75ml/kg of physiological saline, and were perfused with stomach for 6 weeks, once daily.
Western Blot detection
After blood is taken from each group of rats, cervical dislocation is killed, right femur is collected immediately by surgical sampling staff after the death of the rats, and TGF-beta 1 protein expression is determined by a Western Blot method.
(1) Femur tissue sample preparation: the femur tissue of the rat is cut into tiny tissue fragments on ice, 150-250ul of lysis solution is added according to the proportion of each 20mg of tissue, and the mixture is placed into a homogenizer for homogenization until the tissue is completely lysed. Samples generated after lysis were centrifuged for a few minutes and the supernatant was stored in a-80℃refrigerator.
(2) And (5) protein measurement.
(3) Loading: adding a proper amount of loading buffer solution, putting into a boiling water bath, centrifuging after Omin, and taking the supernatant for loading. And then placing the prepared PAGE gel into an electrophoresis tank, adding a proper amount of electrophoresis buffer solution, taking down a comb, and blowing a sample adding hole to avoid influencing sample adding due to residual gel in the hole. Finally, the prepared tissue sample is applied to the corresponding well with a loading gun, and care must be taken not to spill over the loading well.
(4) SDS-PAGE electrophoresis: the gel was separated by 10%, concentrated by 4%, diluted 1X with 6 Xloading buffer, and boiled in boiling water for 5-10 minutes to denature protein, and the protein sample was 50. Mu.g. Electrophoresis conditions: 20 MV/block gel, the bromophenol blue reaches the bottom of the electrophoresis gel to stop, and the transfer film is started.
(5) Transferring: putting a film transfer buffer solution into a enamel tray, soaking a clamp, two sponge pads, filter paper and a soaked film in the buffer solution, opening the clamp to keep the black surface horizontal, firstly, putting a sponge pad on the clamp, putting a layer of filter paper on the clamp, paving a separating glue on the filter paper, covering a PVDF film on the glue after no bubbles are ensured, covering the filter paper on the PVDF film, finally covering the sponge pad, closing the clamp, and performing the whole operation in the film transfer buffer solution to ensure no bubble residues among the layers. The clamp is placed in a groove of a transfer groove, so that the black surface of the clamp faces the black surface of the groove, the white surface of the clamp faces the red surface of the groove, heat is generated during electric transfer, one piece of ice is placed on one side of the groove to cool, and the heat is transferred for 1.5-2 hours at constant pressure of 100V.
(6) Immune response: after the transfer, the PVDF membrane is immediately placed in TBST, rinsed lmin-2min, blocked with 5% TBST-skimmed milk powder, and blocked by shaking on a decolorizing shaker at room temperature for 1-2 hours. The membrane was removed from the blocking solution, washed 3 times with TBST, the primary antibody was diluted in proportion with 5% BSA, then the PVDF membrane was placed in a hybridization bag and reacted with the primary antibody overnight at 4℃and, after the reaction, washed three times with TBST at room temperature on a decolorizing shaker for 10min each. PVDF membrane is placed in 5% skimmed milk containing secondary antibody with corresponding concentration to bind secondary antibody, after 2 hours, the secondary antibody is discarded, and TBST is rinsed for 3 times×10min.
(7) Chemiluminescence, development and fixing: rinsing with TBST for 3 times, adding ECL luminescent reagent onto PVDF film, reacting for 5min, taking into darkroom, exposing with X-ray film, developing, and fixing.
(8) Gel image analysis: the film is scanned or photographed and the molecular weight and net optical density values of the target bands are analyzed using a gel image processing system.
3. Statistical processing
Data are expressed as x-S.+ -. S.E.M. using correlation analysis and Student t-test in Microsoft Excel 2003 software.
4. Experimental results
FIG. 2 shows that the dose-dried prognosis, compared to the normal control group, has a significant difference in the down-regulation of TGF- β1 expression in the model group (P < 0.01); compared with the model group, TGF-beta 1 expression was significantly up-regulated in each of the administration groups, and there was a significant statistical difference (P < 0.01). TGF-beta in bone tissue is mainly synthesized by bone cells, osteoblasts, osteoclasts, chondrocytes, bone marrow cells and the like, is one of the most abundant cytokines in bone tissue, TGF-beta 1 is a cytokine secreted by the osteoblasts and having a strong regulation effect on bones, wherein the TGF-beta 1 subtype has the highest proportion in bones, has the effects of promoting proliferation and differentiation of the osteoblasts and inhibiting activity of the osteoclasts, and is an important coupling factor between bone formation and bone resorption. The research result shows that the expression level of the TGF-beta 1 protein in the rat bone tissue of the model group is obviously reduced compared with that of the normal group, and the expression level of the TGF-beta 1 protein in each administration group is obviously up-regulated, which suggests that the action mechanism of the TGF-beta 1 protein for preventing and treating GIMP is possibly related to the regulation and control of the expression level of the TGF-beta 1 protein by up-regulating the expression level of the TGF-beta 1 protein in each administration group.
Of course, the present application is not limited to the above-described embodiments, and those skilled in the art can make equivalent modifications or substitutions without departing from the spirit of the present application, and these equivalent modifications or substitutions are included in the present application.
Claims (9)
1. A traditional Chinese medicine composition for treating osteoporosis due to qi deficiency and blood stasis is characterized in that: the traditional Chinese medicine is prepared from the following traditional Chinese medicine raw materials in parts by weight: 20-30 parts of astragalus membranaceus, 15-25 parts of codonopsis pilosula, 20-30 parts of bighead atractylodes rhizome, 8-12 parts of poria cocos, 6-10 parts of safflower, 15-25 parts of ligusticum wallichii, 10-16 parts of motherwort herb, 8-12 parts of earthworm, 18-22 parts of dried orange peel, 12-16 parts of notopterygium root, 8-12 parts of radix sileris, 8-12 parts of myrrh, 10-16 parts of trogopterus dung, 3-6 parts of nutgrass galingale rhizome, 12-16 parts of eucommia bark, 10-16 parts of mistletoe, 18-22 parts of rhizoma cibotii, 10-14 parts of rhizoma drynariae, 10-16 parts of oyster and 12-16 parts of dragon bone.
2. The traditional Chinese medicine composition for treating osteoporosis due to qi deficiency and blood stasis according to claim 1, wherein the traditional Chinese medicine composition is characterized by: the traditional Chinese medicine is prepared from the following traditional Chinese medicine raw materials in parts by weight: 25 parts of astragalus, 20 parts of codonopsis pilosula, 25 parts of bighead atractylodes rhizome, 10 parts of poria cocos, 8 parts of safflower, 20 parts of ligusticum wallichii, 13 parts of motherwort herb, 10 parts of earthworm, 20 parts of dried orange peel, 14 parts of notopterygium root, 10 parts of radix sileris, 10 parts of myrrh, 13 parts of trogopterus dung, 5 parts of nutgrass galingale rhizome, 13 parts of eucommia bark, 13 parts of mistletoe, 20 parts of rhizoma cibotii, 12 parts of rhizoma drynariae, 14 parts of oyster and 14 parts of dragon bone.
3. The traditional Chinese medicine composition for treating osteoporosis due to qi deficiency and blood stasis according to claim 1, wherein the traditional Chinese medicine composition is characterized by: the traditional Chinese medicine is prepared from the following traditional Chinese medicine raw materials in parts by weight: 20 parts of astragalus, 25 parts of codonopsis pilosula, 20 parts of bighead atractylodes rhizome, 12 parts of poria cocos, 6 parts of safflower, 25 parts of ligusticum wallichii, 10 parts of motherwort, 12 parts of earthworm, 18 parts of dried orange peel, 16 parts of notopterygium root, 8 parts of radix sileris, 12 parts of myrrh, 10 parts of trogopterus dung, 6 parts of nutgrass galingale rhizome, 12 parts of eucommia bark, 16 parts of mistletoe, 18 parts of rhizoma cibotii, 14 parts of rhizoma drynariae, 10 parts of oyster and 16 parts of dragon bone.
4. The traditional Chinese medicine composition for treating osteoporosis due to qi deficiency and blood stasis according to claim 1, wherein the traditional Chinese medicine composition is characterized by: the traditional Chinese medicine is prepared from the following traditional Chinese medicine raw materials in parts by weight: 30 parts of astragalus, 15 parts of codonopsis pilosula, 30 parts of bighead atractylodes rhizome, 8 parts of poria cocos, 10 parts of safflower, 15 parts of ligusticum wallichii, 16 parts of motherwort, 8 parts of earthworm, 22 parts of dried orange peel, 12 parts of notopterygium root, 12 parts of radix sileris, 8 parts of myrrh, 16 parts of trogopterus dung, 3 parts of nutgrass galingale rhizome, 16 parts of eucommia bark, 10 parts of mistletoe, 22 parts of rhizoma cibotii, 10 parts of rhizoma drynariae, 16 parts of oyster and 12 parts of dragon bone.
5. The traditional Chinese medicine composition for treating osteoporosis due to qi deficiency and blood stasis according to any one of claims 1 to 4, wherein: the preparation method comprises the following steps:
(1) Decocting Concha Ostreae and Os Draconis with 8 times of water for 1 hr;
(2) Extracting Carthami flos, rhizoma Ligustici Chuanxiong, pericarpium Citri Tangerinae, myrrha, oletum Trogopterori, and rhizoma Cyperi by steam distillation, and extracting volatile oil;
(3) Adding the residues and other medicinal materials obtained after the steam distillation extraction of the safflower, the ligusticum wallichii, the dried orange peel, the myrrh, the trogopterus dung and the rhizoma cyperi in the step (1) into the decoction of the oyster and the dragon bone in the step (2), adding the steam distillation decoction in the step (2), supplementing 10 times of total weight of water of all medicinal materials, decocting for three times, combining the water extracts each time for 2 hours, filtering to obtain a mixed water extract, heating and concentrating to obtain a mixed water extract concentrate, concentrating and drying under reduced pressure, and spraying the safflower, the ligusticum wallichii, the dried orange peel, the myrrh, the trogopterus dung and the rhizoma cyperi volatile oil obtained in the step (2);
(4) Adding adjuvants, and making into tablet, capsule, or granule.
6. The application of a Chinese medicinal composition for treating osteoporosis due to qi deficiency and blood stasis in claim 1 in preparing medicaments for treating osteoporosis due to qi deficiency and blood stasis.
7. The application of a Chinese medicinal composition for treating osteoporosis due to qi deficiency and blood stasis in claim 1 in preparing medicaments for treating osteoporosis due to qi deficiency and blood stasis, wherein the Chinese medicinal composition can improve TGF-beta 1, BGP and ALP levels in serum.
8. The application of a Chinese medicinal composition for treating osteoporosis due to qi deficiency and blood stasis in claim 1 in preparing a medicament for treating osteoporosis due to qi deficiency and blood stasis, wherein the Chinese medicinal composition can reduce the TRACP level in serum.
9. The application of a Chinese medicinal composition for treating osteoporosis due to qi deficiency and blood stasis in claim 1 in preparing a medicament for treating osteoporosis due to qi deficiency and blood stasis, wherein the Chinese medicinal composition can improve the expression of TGF-beta 1 protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210408947.0A CN115006480B (en) | 2022-04-19 | 2022-04-19 | Traditional Chinese medicine composition for treating osteoporosis and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210408947.0A CN115006480B (en) | 2022-04-19 | 2022-04-19 | Traditional Chinese medicine composition for treating osteoporosis and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115006480A CN115006480A (en) | 2022-09-06 |
| CN115006480B true CN115006480B (en) | 2023-08-22 |
Family
ID=83066611
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210408947.0A Active CN115006480B (en) | 2022-04-19 | 2022-04-19 | Traditional Chinese medicine composition for treating osteoporosis and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115006480B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583069A (en) * | 2004-06-03 | 2005-02-23 | 陈根生 | Medicine for osteoporosis |
| CN104840937A (en) * | 2015-06-15 | 2015-08-19 | 王艳娣 | Traditional Chinese medicine preparation for treating osteoporosis |
| CN112717047A (en) * | 2021-03-09 | 2021-04-30 | 西安医学院第二附属医院 | Medicine for treating osteoporosis and treatment method thereof |
-
2022
- 2022-04-19 CN CN202210408947.0A patent/CN115006480B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583069A (en) * | 2004-06-03 | 2005-02-23 | 陈根生 | Medicine for osteoporosis |
| CN104840937A (en) * | 2015-06-15 | 2015-08-19 | 王艳娣 | Traditional Chinese medicine preparation for treating osteoporosis |
| CN112717047A (en) * | 2021-03-09 | 2021-04-30 | 西安医学院第二附属医院 | Medicine for treating osteoporosis and treatment method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 四君逐瘀汤"治疗原发性骨质疏松症40 例临床研究;朱辉军;《江苏中医药》;第45卷(第1期);33-34 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115006480A (en) | 2022-09-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103417904B (en) | Traditional Chinese medicine composition for treating chronic cheilitis and preparing method thereof | |
| KR101787432B1 (en) | Method for manufacturing oriental medicine composition which improves menorrhagia and dysmenorrhea comprising fermented velvet antler and yeast hydrolysate | |
| CN104187172B (en) | Functional feed for treating enterogastritis diseases of turtles and preparation method of functional feed | |
| CN104305205A (en) | Cuscuta chinensis oral liquid for improving immunity and preparation method of cuscuta chinensis oral liquid | |
| CN103055285B (en) | Traditional Chinese medicine for treating forepart hepetocirrhosis and preparation method thereof | |
| CN108379521A (en) | Prostate cancer therapy liquid and preparation method thereof | |
| CN103041262B (en) | Traditional Chinese medicine enema for curing chronic prostatitis and preparing method thereof | |
| CN112156113A (en) | Method for cultivating bezoar in ox gall by Chinese herbal medicine induction | |
| CN104223297A (en) | Aging-delaying health schisandra chinensis oral solution and preparation method thereof | |
| CN115006480B (en) | Traditional Chinese medicine composition for treating osteoporosis and preparation method thereof | |
| CN104998197A (en) | Traditional Chinese medicine composition for treating liver cancer | |
| CN104826012A (en) | Traditional Chinese medicine preparation for pituitary tumor surgery postoperative nursing and preparation method thereof | |
| CN104189413A (en) | Medicine composition for treating enterogastritis diseases of turtles and preparation method of medicine composition | |
| CN105998246A (en) | Externally-used traditional Chinese medicine paste for treating painful heel and preparation method thereof | |
| CN104983951A (en) | Drug for treating sicca syndrome | |
| CN115554372B (en) | Traditional Chinese medicine for treating primary fibromyalgia syndrome and preparation method and application thereof | |
| CN114767802B (en) | Traditional Chinese medicine composition for treating excessive phlegm-dampness type metabolic hypertension caused by diet loss and preparation method and application thereof | |
| CN105998532A (en) | Preparation method for traditional Chinese medicine for treating Qi-stagnancy and blood stasis painful heel and composition of traditional Chinese medicine | |
| CN114796434A (en) | Pill for keeping healthy qi and pathogenic factors dry | |
| CN105998448A (en) | Method for preparing mulberry oral solution for improving immunity and oral solution prepared by same | |
| CN106107152A (en) | A kind of feed additive improving meat rabbit immunity and preparation method thereof | |
| CN105920189A (en) | Traditional Chinese medicine composition for treating talalgia due to liver-kidney deficiency and preparation method thereof | |
| CN104922595A (en) | Traditional Chinese medicinal preparation for herpes zoster and preparation method thereof | |
| CN104800519A (en) | Medicine for treating breast hyperplasia with disharmony of thoroughfare and conception vessels and preparation method of medicine | |
| CN113975361A (en) | Traditional Chinese medicine soft capsule containing amomum oil and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |