CN108379521A - Prostate cancer therapy liquid and preparation method thereof - Google Patents

Prostate cancer therapy liquid and preparation method thereof Download PDF

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CN108379521A
CN108379521A CN201810526628.3A CN201810526628A CN108379521A CN 108379521 A CN108379521 A CN 108379521A CN 201810526628 A CN201810526628 A CN 201810526628A CN 108379521 A CN108379521 A CN 108379521A
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prostate cancer
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cancer therapy
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郭巧琴
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Beijing Kang Fen Traditional Chinese Medicine Research Institute
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Beijing Kang Fen Traditional Chinese Medicine Research Institute
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Abstract

The invention discloses a kind of prostate cancer therapy liquid and preparation method thereof.The prostate cancer therapy liquid includes Lowiaceae plant extraction concentrate and Chinese herbal medicine combination decocting liquid.Prostate cancer therapy liquid of the present invention, adjust body's immunity, by changing the body vivo environment of patients with prostate cancer, the disorder of the various physiological functions caused by the occurrence and development of prostate cancer is adjusted, growth of cancer cells is inhibited, promote apoptosis of tumor cells, realize the unapproachable late result of other treatment method and long-term survival rate, and treatment is milder, it is small to the injury of patient, risk is low, can mitigate the burden of patients with prostate cancer.

Description

Prostate cancer therapy liquid and preparation method thereof
Technical field
The present invention relates to pharmaceutical techniques fields, more particularly to prostate cancer therapy liquid and preparation method thereof.
Background technology
Prostate cancer refers to being happened at the epithelial malignancy of prostate.WHO in 2004《Urinary system and male genetic Organ tumor pathology and science of heredity》Including on gland cancer (acinar adenocarcinoma), duct adenocarcinoma, urinary tract in middle prostate cancer histological type Skin cancer, squamous cell carcinoma, adenosquamous carcinoma.Wherein adenocarcinoma of the prostate accounts for 95% or more, and therefore, the prostate cancer usually described in us is just It refer to adenocarcinoma of the prostate.China's tumour registration area prostate-cancer incidence in 2012 is 9.92/10 ten thousand, row male malignancy The 6th of incidence.Age of onset before 55 years old be in reduced levels, gradually risen after 55 years old, incidence with the age increasing It grows and increases, the peak age is 70~80 years old.A little earlier, the age is less than 55 years old familial inheritance type patients with prostate cancer age of onset Patient accounts for 43%.
The generation of prostate cancer is related with inherent cause.If in family without suffer from prostate cancer person relative risk be 1, Absolute danger level is 8；The relative risk that then genotype prostate cancer family member suffers from prostate cancer is 5, and absolute danger level is 35~45.In addition, the morbidity of prostate cancer is related with sexuality, eating habit.The more person of sexuality suffers from the risk of prostate cancer Increase.High fat diet also has certain relationship with morbidity.In addition, the morbidity of prostate cancer and race, area, religious belief are possible It is related.
Prostate cancer early stage is often asymptomatic, and with the development of tumour, symptom can be summarized as two major classes caused by prostate cancer：
1, pressure symptom
Gradual increased prostate gland urethra can cause progressive dysuria, show as urine line is thin, range is short, Bradyuria, uroflow interrupt, urine after the sound of rain pattering, urination not to the utmost, trouble urinating, in addition, also frequent micturition, urgent urination, enuresis nocturna increase, even The urinary incontinence.Oncothlipsis rectum can cause to have difficulty in passing one's motions or intestinal obstruction, can also oppress vas deferens and cause absence of ejaculation, pressuring nerve Cause perineum pain, and can be radiated to sciatic nerve.
2, metastasis symptom
Prostate cancer can invade and bladder, seminal vesicle, nervus vasculairs beam, causes blood urine, blood sperm, impotence.Lymph node transfers prognosi It can cause double lower limb oedema.Bone tumour often easily occurs for prostate cancer, causes ostalgia or pathologic fracture, paraplegia.Prostate cancer It can invade and marrow causes anaemia or whole blood as reducing.
As other oncotherapies, operation, radiotherapy, chemotherapy are three big means of prostate cancer therapy, though these methods So part lesion can be made temporarily to be controlled, but while killing tumor cell, also damage the certain functions of human body, reduced Patients ' life quality, and leave potential risk factor for the multiple method of tumour or transfer.Relatively more generally acknowledged another kind is swollen at present Tumor therapy is traditional Chinese medicine complex treatment, and traditional Chinese medicine and active components of plants are especially aided with while Radiotherapy chemotherapy Kidney and spleen invigorating, righting solid (training) this etc. be considered as a kind of base therapy.
Lowiaceae plant contains palmitic acid, sitosterol, monoglyceride, Hu screw rod glycosides isoreactivity substance, and antioxidant activity is very To vitamin E is better than, had potential application in anti-tumor aspect.But its root skin portion fibroid is stronger, is carrying During taking, active constituent, which hardly results in, effectively to be discharged.Inventor has found on the basis of Vietnam carries out long-term clinical trials The extract liquor of Lowiaceae plant has good antitumor efficacy.
Invention content
Purpose of the present invention is to be achieved through the following technical solutions ground：
The first technical problem to be solved by the present invention is to provide a kind of prostate cancer therapy liquid.
The invention discloses a kind of prostate cancer therapy liquid, including Lowiaceae plant extraction concentrate and Chinese herbal medicine to combine decocting Liquid.
Further, including 5~15 parts by weight of Lowiaceae plant extraction concentrate and Chinese herbal medicine combine 10~30 weight of decocting liquid Part；
The Chinese herbal medicine combination decocting liquid is prepared by following steps：
(1) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae are rinsed with water, and remove surface silt, and cold standby is set in drying；
(2) step (1) is weighed treated 8~15 parts by weight of Radix Astragali, 3~7 parts by weight of Chinese yew, 2~4 weight of Radix Codonopsis Part, 2~4 parts by weight of Rhizoma Atractylodis Macrocephalae, 5~10 parts by weight of Poria cocos, 6~10 parts by weight of matrimony vine, 3~7 parts by weight of Semen Cuscutae, Chinese lobelia 2~4 Parts by weight, 5~15 parts by weight of Radix Glycyrrhizae, 2~5 parts by weight of the fruit of glossy privet, 3~7 parts by weight of Herba Epimedii, 8~15 parts by weight of Cortex Phellodendri, Radix Curcumae 8~15 parts by weight；
(3) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae crush respectively, and sieving is uniformly mixed so as to obtain herb powder；100~150 grams of herb powder is taken, water is added to carry out 2~3 Secondary decoction；600~800mL of 1st decoction plus water, the 2nd~3 decoction adds 300~400mL of water respectively, every time in 70 when decoction ~80 DEG C decoct 30~40 minutes, and after the completion of decoction, filtering, collects the dregs of a decoction and decoction liquor, the dregs of a decoction are decocted next time again；It closes And the decoction liquor decocted every time, it is concentrated to give the Chinese herbal medicine combination decocting liquid of 1~1.2g of concentration crude drugs/mL；Set cold standby.
Radix Astragali, Classification system：Astragalus membranaceus(Fisch.)Bunge..Medicine elementary source is pulse family grass The root of this plant astragalus mongolicus, Astragalus membranacus.
Chinese yew, Classification system：Taxus chinensis(Pilger)Rehd.Taxus chinensis(Pilger) Rehd..Chinese yew is acknowledged as plants for anticancer, and the taxol being extract from Chinese yew protrudes cancer curative effect, is referred to as " controlling Treat the last line of defense of cancer ".Taxol has unique resistance mechanisms to tumour, while significantly inhibiting the work of tumour again With.
Radix Codonopsis, Classification system：Codonopsis pilosula(Franch.)Nannf..With tonifying middle-Jiao and Qi, invigorating the spleen benefit The effect of lung.Radix Codonopsis has strengthen immunity, expansion blood vessel, decompression, improves the effects that microcirculation, enhancing hematopoiesis function.In addition right Leucocyte decline caused by chemotherapy radiotherapy has castering action.
Rhizoma Atractylodis Macrocephalae, Classification system：Atractylodes macrocephala Koidz., Wen Xinlie bitter in taste have eliminating dampness, change It is turbid, analgesic the benefits of.
Poria cocos, Classification system：Wolfiporia cocos.Chinese medicine thinks that Poria cocos slightly sweet flavor is flat, enters the heart, lung, the spleen channel.Tool There is the effect of eliminating dampness and diuresis, strengthening the spleen and stomach, antitoxic heart-soothing and sedative.
Matrimony vine, property sweet and neutral.Has an impact effect to immune function.Lycium barbarum is uniquely to be loaded into《Traditional Chinese medicines in versions in 2010 Allusion quotation》Matrimony vine kind.
Semen Cuscutae, Classification system：Cuscuta chinensis.Semen Cuscutae is sweet, warm, and kidney,liver,spleen is returned to pass through, and has nourishing liver The effect of kidney, astringent sperm contracting urine, tocolysis, improving eyesight, antidiarrheal, begins to carry《Sheng Nong's herbal classic》, it is listed in top grade.
Chinese lobelia, Classification system：Lobelia chinensis Lour..Herb is medicinal, contains a variety of alkaloids, there is heat-clearing The benefits of removing toxic substances, inducing diuresis to remove edema, control venomous snake bite, cirrhotic ascites, advanced schistosomiasis ascites, appendicitis effect etc..
Radix Glycyrrhizae, Classification system：Glycyrrhiza uralensis Fisch.Pulse family, Glycyrrhiza perennial herb, are one Kind help Chinese herbal medicine.《Compendium of Materia Medica》Described in: Radix Glycyrrhizae is monarch in all medicines, controls 72 kinds of mammary calculus poison, and solution 1,002 is by every means careless The wooden poison, many medicines that reconcile are active therefore have number of state always.Radix Glycyrrhizae, Radix Salviae Miltiorrhizae, Classification system in embodiment：Salvia miltiorrhiza Bunge.For dicotyledon Lamiaceae Salvia category perennial vertical herbage plant.Root is used as medicine, and contains tanshinone, logical for robustness Through agent, there are the effectiveness such as dissolving stasis, raw new, promoting blood circulation, menstruation regulating.
The fruit of glossy privet, alias glossy privet reality, purpleflower holly fruit, wax tree, mouse Chinese catalpa, are Oleaceae plants glossy privet Ligustrumlucidum Ait. dry mature fruit.It is kidney tonifying enriching yin simply, the Chinese medicine to nourish the liver to improve visual acuity, it is early that kidney deficiency and liver, dizzy syrigmus, hair can be controlled The illnesss such as two blurred vision of bletilla paste.
Herba Epimedii, Classification system：Epimedium sagittatum are the plants of Berberidaceae Epimedium.It can be with kidney tonifying Sun, strengthening the bones and muscles, wind-damp dispelling.For impotence and seminal emission, muscles and bones impotence, arthralgia pain due to rheumatism, numbness contracture；Climacteric hypertension.
Cortex Phellodendri, Classification system：Phellodendron amurense Rupr..Bitter, it is cold in nature.Clearing heat and detoxicating, purging intense heat is dry It is wet.Cure mainly the inflammation such as acute bacillary dysentery, chordapsus, acute icteric hepatitis, urinary system infection contamination.
Radix Curcumae is zingiberaceous plant RADIX CURCUMAE Curcuma rcenyujin Y, H.Chenet C.Ling, turmeric Curcuma LongaL., Guangxi zedoary Curcumakwangsiensis S.G.Lee et C.F.Liang or zedoary Curcuma pha The dried root of eocaulis Val..
As one of technical scheme of the present invention, the Lowiaceae plant extraction concentrate obtains by the following method：
(1) 20~50 parts by weight of bark of Lowiaceae plant, 20~50 parts by weight of tree stem, 20~50 parts by weight of leaf are done It is dry, it crushes respectively, is sieved, is uniformly mixed so as to obtain mixture；
(2) by mixture and extraction solvent with solid-to-liquid ratio 1：(1~10) (g/mL) mixing, in 50~60 DEG C of ultrasonic extractions 40~100 minutes, extracting solution is collected, the Lowiaceae plant extraction concentrate is obtained.
As the two of technical scheme of the present invention, the Lowiaceae plant extraction concentrate obtains by the following method：
(1) 20~50 parts by weight of bark of Lowiaceae plant, 20~50 parts by weight of tree stem, 20~50 parts by weight of leaf are done It is dry, it crushes respectively, is sieved, is uniformly mixed so as to obtain mixture；
(2) mixture is immersed 30~40 DEG C, in the sodium hydrate aqueous solution of mass fraction 5~20%, dipping 30~40 After minute, filtering takes filter cake；Filter cake is washed with water to washing lotion to be in neutrality, is drained, extraction raw material is obtained；
(3) by extraction raw material and extraction solvent with solid-to-liquid ratio 1：(1~10) (g/mL) mixing, in 50~60 DEG C of ultrasounds Extracting solution is collected in extraction 40~100 minutes；
(4) extracting solution is obtained into the Lowiaceae plant extraction concentration by the ultrafiltration through membranes of 10~100KD of molecular cut off Liquid.
As the three of technical scheme of the present invention, the Lowiaceae plant extraction concentrate obtains by the following method：
(1) 20~50 parts by weight of bark of Lowiaceae plant, 20~50 parts by weight of tree stem, 20~50 parts by weight of leaf are done It is dry, it crushes respectively, is sieved, is uniformly mixed so as to obtain mixture；
(2) mixture is immersed 30~40 DEG C, in the sodium hydroxide solution of mass fraction 5~20%, impregnates 30~40 points Zhong Hou, filtering, Washing of Filter Cake to neutrality drains, obtains extraction raw material；
(3) extraction is placed in raw material in refiner, the ethyl alcohol of volume fraction 50~80%, extraction raw material and second is added The solid-to-liquid ratio of alcohol is 1：(2~5) (g/mL) is extracted 3~4 times in 20~30 DEG C of homogenate, is extracted 3~5 minutes every time；By extracting solution Filtered through gauze is used after merging, collects extracting solution A and filter cake；
(4) filter cake and extraction solvent obtained step (3) is with solid-to-liquid ratio 1：(1~10) (g/mL) mixing, in 50~ 60 DEG C of ultrasonic extractions 40~100 minutes collect extracting solution B；
(5) extracting solution A and extracting solution B are merged, vacuum distillation removes ethyl alcohol, obtains extracting solution；
(6) extracting solution is obtained into the Lowiaceae plant extraction concentration by the ultrafiltration through membranes of 10~100KD of molecular cut off Liquid.
Homogenate is that biological tissue is carried out tissue homogenate or defibrination by the way that solvent is added, to extract in living tissue effectively A kind of method of ingredient.By this method, the substances such as terpenol, flavones in Lowiaceae plant can be extracted, is played Good extraction effect.
The Lowiaceae plant both can be Streliziaceae, Cannaceae, heliconiaceae, one or more of Musaceae, Other kinds of Lowiaceae plant can also be selected.
The Extraction solvent is the sodium-chloride water solution of 3-10%, 35-55 DEG C of water or mass fraction.
Preferably, the prostate cancer therapy liquid further includes 0.1~1 parts by weight black garlic extracting solution and/or safflower pulp liquid. It is further preferred that the weight ratio of the black garlic extracting solution and safflower pulp liquid is 1：1.
The processing method of the black garlic extracting solution is：Fresh garlic is pre-processed；By pretreated garlic in 60~80 DEG C, place 20~45 days in the environment of humidity 40~70%, obtain black garlic；Black garlic is weighed, black garlic is broken into paste, is added black The distilled water that 1~2 times of garlic weight obtained black garlic extracting solution in 60~70 DEG C of ultrasonic extractions 20~40 minutes；
The processing method of the safflower pulp liquid is：Fresh safflower fruit is selected, is crushed, squeezes the juice, collects juice；By juice With filtered through gauze, filtrate is taken；Filtrate sterilizes, and sets cold standby.
The pretreatment mode of the fresh garlic is：Fresh garlic is small in -20~-18 DEG C of cryogenic freezing processing 24~36 When, or fresh garlic is handled 3~5 hours in 90~100 DEG C of water high temperature boilings.
The second technical problem to be solved by the present invention is to provide a kind of preparation method of prostate cancer therapy liquid.
The preparation method of the prostate cancer therapy liquid, includes the following steps：By Lowiaceae plant extraction concentrate and medium-height grass Medicine combine decocting liquid (or Chinese herbal medicine combination decocting liquid and black garlic extracting solution and/or safflower fruit liquid) be uniformly mixed to get.
The hyper-proliferative of cell is one of biological characteristics of malignant tumour.The drug of prostate cancer is treated in the prior art It is divided into following two situations：It is relatively low to the selectivity of tumour cell, it is thin to normally organizing while injuring tumor cell Born of the same parents also have to be damaged to a certain extent；Alternatively, being not directly placed on cancer cell, mitigate toxic and side effects of chemoradiotherapy, drug effect is slow.This hair The bright prostate cancer therapy liquid while changing the breeding cycle of prostate gland cancer cell, is adjusted by direct cytotoxicity The growing environment of prostate gland cancer cell inhibits the growth and breeding of tumour with number of ways.
Prostate cancer therapy liquid of the present invention utilizes traditional Chinese medicine theory and Immunization Update theory and cell differentiation Proliferating cycle is theoretical, adjusts body's immunity, by changing the body vivo environment of patient, adjust because prostate cancer generation and The disorder of various physiological functions caused by development, inhibits growth of cancer cells, promotes apoptosis of tumor cells, realizes other treatment method Unapproachable late result and long-term survival rate, and treatment is milder, and small to the injury of patient, risk is low, can subtract The burden of light patients with prostate cancer.
Specific implementation mode
It is further illustrated the present invention below by the mode of embodiment, but does not therefore limit the present invention to the reality It applies among a range.
In following embodiments,
Lowiaceae plant in embodiment selects the Banana tree for originating from Nanning, Musaceae.
Radix Astragali in embodiment, source area Anhui are purchased from gloomy medicine company sale Co., Ltd in Bozhou City woods.
Chinese yew in embodiment, source area Sichuan are purchased from Sichuan Fu Zheng medicine companies Co., Ltd.
Radix Codonopsis in embodiment, source area Yunnan are purchased from Xichou County Xiang Guang medicinal materials Co., Ltd.
Rhizoma Atractylodis Macrocephalae in embodiment, source area Anhui are purchased from gloomy medicine company sale Co., Ltd in Bozhou City woods.
Poria cocos in embodiment, source area Yunnan are purchased from ten thousand visitor of Bozhou City and carry out Chinese medicine wholesale house.
Matrimony vine in embodiment, source area Ningxia are purchased from Shang Zhi commerce and trade Co., Ltd of Anhui Province.
Semen Cuscutae in embodiment, the source area Inner Mongol are purchased from Bozhou City Xin Yuan Chinese medicines Science and Technology Ltd..
Chinese lobelia in embodiment, source area Jiangsu are purchased from Bozhou City often rich medicine company sale Co., Ltd.
Radix Glycyrrhizae in embodiment, the source area Inner Mongol are purchased from Bozhou Bai Chuan pharmaceutcal corporation, Ltds.
The fruit of glossy privet in embodiment, source area Gansu are purchased from gloomy medicine company sale Co., Ltd in Bozhou City woods.
Herba Epimedii in embodiment, source area Yunnan are purchased from Bozhou City Xin Yuan Chinese medicines Science and Technology Ltd..
Cortex Phellodendri in embodiment, source area Sichuan are purchased from gloomy medicine company sale Co., Ltd in Bozhou City woods.
Radix Curcumae in embodiment, source area Guangxi are purchased from gloomy medicine company sale Co., Ltd in Bozhou City woods.
Filtering gauze in embodiment, Nantong Chengtong textile mills provide, yarn count 40*40, plain weave, density 28 × 24, breadth 105,100% cotton of ingredient.
Ultrafiltration membrane in embodiment, Hangzhou Jiu Ling Science and Technology Ltd.s provide, material polyether sulfone, molecular cut off 10KD.
Garlic in embodiment is purchased from Guangxi Chang Sheng food industry Co., Ltd.
The title that safflower fruit (Malus asiatica) has Chinese pear-leaved crabapple, because color is scarlet, so also there is person, it is premium, safflower Fruit belongs to the plant of rose family Malus.《Kaibao Bencao》Cloud：It " can not eat, generate heat puckery gas, makes us liking to sleep, and feel cold phlegm, raw furuncle more Sore, arteries and veins close not all right.Safflower fruit in embodiment, kind sweet tea sand are red.
Embodiment 1
Prostate cancer therapy liquid, including 10 parts by weight of Lowiaceae plant extraction concentrate and Chinese herbal medicine combine 30 weight of decocting liquid Part.
Prepare prostate cancer therapy liquid：Lowiaceae plant extraction concentrate and Chinese herbal medicine combination decocting liquid are uniformly mixed, i.e., .
The Chinese herbal medicine combination decocting liquid is prepared by following steps：
(1) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae are rinsed with water, remove surface silt, in 50 DEG C dry 4 hours, be cooled in air 30 DEG C it is spare；
(2) step (1) is weighed treated 10 parts by weight of Radix Astragali, 5 parts by weight of Chinese yew, 2 parts by weight of Radix Codonopsis, 2 weight of Rhizoma Atractylodis Macrocephalae Measure part, 5 parts by weight of Poria cocos, 8 parts by weight of matrimony vine, 3 parts by weight of Semen Cuscutae, 2 parts by weight of Chinese lobelia, 10 parts by weight of Radix Glycyrrhizae, the fruit of glossy privet 2 Parts by weight, 3 parts by weight of Herba Epimedii, 8 parts by weight of Cortex Phellodendri, 10 parts by weight of Radix Curcumae；
(3) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae crush respectively, cross 80 mesh sieve, are uniformly mixed so as to obtain herb powder；100 grams of herb powder is taken, is added water to cook, is decocted altogether It boils three times；1st decoction plus water 700mL, the 2nd time and the 3rd time decoction add water 300mL respectively, every time in 80 DEG C of decoctions when decoction It 30 minutes, after the completion of decoction, with filtered through gauze, collects the dregs of a decoction and decoction liquor, the dregs of a decoction is decocted next time again；Merging is decocted three times The decoction liquor boiled, the Chinese herbal medicine that concentration 1g crude drugs/mL is concentrated under reduced pressure to give in 50 DEG C, absolute pressure 0.01MPa combine decocting liquid； Be cooled in air 30 DEG C it is spare.
The Lowiaceae plant extraction concentrate obtains by the following method：
It is (1) 30 parts by weight of bark of Lowiaceae plant, 25 parts by weight of tree stem, 20 parts by weight of leaf are 4 hours dry in 60 DEG C, It crushes respectively, crosses 40 mesh sieve, be uniformly mixed so as to obtain mixture；
(2) by mixture and water with solid-to-liquid ratio 1：6 (g/mL) mixings, in 50 DEG C in ultrasonic power 300W, supersonic frequency It is extracted 65 minutes under conditions of 20kHz, collects extracting solution, obtain the Lowiaceae plant extraction concentrate.
Embodiment 2
Prostate cancer therapy liquid, including 10 parts by weight of Lowiaceae plant extraction concentrate and Chinese herbal medicine combine 30 weight of decocting liquid Part.
Prepare prostate cancer therapy liquid：Lowiaceae plant extraction concentrate and Chinese herbal medicine combination decocting liquid are uniformly mixed, i.e., .
The Chinese herbal medicine combination decocting liquid is prepared by following steps：
(1) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae are rinsed with water, remove surface silt, in 50 DEG C dry 4 hours, be cooled in air 30 DEG C it is spare；
(2) step (1) is weighed treated 10 parts by weight of Radix Astragali, 5 parts by weight of Chinese yew, 2 parts by weight of Radix Codonopsis, 2 weight of Rhizoma Atractylodis Macrocephalae Measure part, 5 parts by weight of Poria cocos, 8 parts by weight of matrimony vine, 3 parts by weight of Semen Cuscutae, 2 parts by weight of Chinese lobelia, 10 parts by weight of Radix Glycyrrhizae, the fruit of glossy privet 2 Parts by weight, 3 parts by weight of Herba Epimedii, 8 parts by weight of Cortex Phellodendri, 10 parts by weight of Radix Curcumae；
(3) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae crush respectively, cross 80 mesh sieve, are uniformly mixed so as to obtain herb powder；100 grams of herb powder is taken, is added water to cook, is decocted altogether It boils three times；1st decoction plus water 700mL, the 2nd time and the 3rd time decoction add water 300mL respectively, every time in 80 DEG C of decoctions when decoction It 30 minutes, after the completion of decoction, with filtered through gauze, collects the dregs of a decoction and decoction liquor, the dregs of a decoction is decocted next time again；Merging is decocted three times The decoction liquor boiled, the Chinese herbal medicine that concentration 1g crude drugs/mL is concentrated under reduced pressure to give in 50 DEG C, absolute pressure 0.01MPa combine decocting liquid； Be cooled in air 30 DEG C it is spare.
The Lowiaceae plant extraction concentrate obtains by the following method：
It is (1) 30 parts by weight of bark of Lowiaceae plant, 25 parts by weight of tree stem, 20 parts by weight of leaf are 4 hours dry in 60 DEG C, It crushes respectively, crosses 40 mesh sieve, be uniformly mixed so as to obtain mixture；
(2) mixture is immersed 40 DEG C, in the sodium hydrate aqueous solution of mass fraction 5%, dipping uses after 30 minutes 100 mesh filter-cloth filterings, take filter cake；Filter cake is washed with water to washing lotion to be in neutrality, is drained, extraction raw material is obtained；
(3) by extraction raw material and water with solid-to-liquid ratio 1：6 (g/mL) mixings, in 50 DEG C in ultrasonic power 300W, supersonic frequency It is extracted 65 minutes under conditions of rate 20kHz, collects extracting solution；
(4) extracting solution is obtained into the Lowiaceae plant extraction concentrate by the ultrafiltration through membranes of molecular cut off 10KD.
Embodiment 3
Prostate cancer therapy liquid, including 10 parts by weight of Lowiaceae plant extraction concentrate and Chinese herbal medicine combine 30 weight of decocting liquid Part.
Prepare prostate cancer therapy liquid：Lowiaceae plant extraction concentrate and Chinese herbal medicine combination decocting liquid are uniformly mixed, i.e., .
The Chinese herbal medicine combination decocting liquid is prepared by following steps：
(1) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae are rinsed with water, remove surface silt, in 50 DEG C dry 4 hours, be cooled in air 30 DEG C it is spare；
(2) step (1) is weighed treated 10 parts by weight of Radix Astragali, 5 parts by weight of Chinese yew, 2 parts by weight of Radix Codonopsis, 2 weight of Rhizoma Atractylodis Macrocephalae Measure part, 5 parts by weight of Poria cocos, 8 parts by weight of matrimony vine, 3 parts by weight of Semen Cuscutae, 2 parts by weight of Chinese lobelia, 10 parts by weight of Radix Glycyrrhizae, the fruit of glossy privet 2 Parts by weight, 3 parts by weight of Herba Epimedii, 8 parts by weight of Cortex Phellodendri, 10 parts by weight of Radix Curcumae；
(3) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae crush respectively, cross 80 mesh sieve, are uniformly mixed so as to obtain herb powder；100 grams of herb powder is taken, is added water to cook, is decocted altogether It boils three times；1st decoction plus water 700mL, the 2nd time and the 3rd time decoction add water 300mL respectively, every time in 80 DEG C of decoctions when decoction It 30 minutes, after the completion of decoction, with filtered through gauze, collects the dregs of a decoction and decoction liquor, the dregs of a decoction is decocted next time again；Merging is decocted three times The decoction liquor boiled, the Chinese herbal medicine that concentration 1g crude drugs/mL is concentrated under reduced pressure to give in 50 DEG C, absolute pressure 0.01MPa combine decocting liquid； Be cooled in air 30 DEG C it is spare.
The Lowiaceae plant extraction concentrate obtains by the following method：
It is (1) 30 parts by weight of bark of Lowiaceae plant, 25 parts by weight of tree stem, 20 parts by weight of leaf are 4 hours dry in 60 DEG C, It crushes respectively, crosses 40 mesh sieve, be uniformly mixed so as to obtain mixture；
(2) mixture is immersed 40 DEG C, in the sodium hydrate aqueous solution of mass fraction 5%, dipping uses after 30 minutes 100 mesh filter-cloth filterings, take filter cake；Filter cake is washed with water to washing lotion to be in neutrality, is drained, extraction raw material is obtained；
(3) extraction is placed in raw material in refiner, the ethyl alcohol of volume fraction 50% is added, extraction raw material and ethyl alcohol Solid-to-liquid ratio is 1：2 (g/mL) are homogenized 3 times in 26 DEG C, every time extraction 5 minutes；Filtered through gauze, collection is used to carry after extracting solution is merged Take liquid A and filter cake；
(4) filter cake and water obtained step (3) is with solid-to-liquid ratio 1：6 (g/mL) mixings, in 50 DEG C ultrasonic power 300W, It is extracted 65 minutes under conditions of supersonic frequency 20kHz, collects extracting solution B；
(5) extracting solution A and extracting solution B are merged, vacuum distillation removes ethyl alcohol, obtains extracting solution；
(6) extracting solution is obtained into the Lowiaceae plant extraction concentrate by the ultrafiltration through membranes of molecular cut off 10KD.
Embodiment 4
Prostate cancer therapy liquid, including 10 parts by weight of Lowiaceae plant extraction concentrate, Chinese herbal medicine combine 30 weight of decocting liquid Part and 0.6 parts by weight of black garlic extracting solution.
Prepare prostate cancer therapy liquid：By Lowiaceae plant extraction concentrate, Chinese herbal medicine combination decocting liquid and black garlic extracting solution Be uniformly mixed to get.
The Chinese herbal medicine combination decocting liquid is prepared by following steps：
(1) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae are rinsed with water, remove surface silt, in 50 DEG C dry 4 hours, be cooled in air 30 DEG C it is spare；
(2) step (1) is weighed treated 10 parts by weight of Radix Astragali, 5 parts by weight of Chinese yew, 2 parts by weight of Radix Codonopsis, 2 weight of Rhizoma Atractylodis Macrocephalae Measure part, 5 parts by weight of Poria cocos, 8 parts by weight of matrimony vine, 3 parts by weight of Semen Cuscutae, 2 parts by weight of Chinese lobelia, 10 parts by weight of Radix Glycyrrhizae, the fruit of glossy privet 2 Parts by weight, 3 parts by weight of Herba Epimedii, 8 parts by weight of Cortex Phellodendri, 10 parts by weight of Radix Curcumae；
(3) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae crush respectively, cross 80 mesh sieve, are uniformly mixed so as to obtain herb powder；100 grams of herb powder is taken, is added water to cook, is decocted altogether It boils three times；1st decoction plus water 700mL, the 2nd time and the 3rd time decoction add water 300mL respectively, every time in 80 DEG C of decoctions when decoction It 30 minutes, after the completion of decoction, with filtered through gauze, collects the dregs of a decoction and decoction liquor, the dregs of a decoction is decocted next time again；Merging is decocted three times The decoction liquor boiled, the Chinese herbal medicine that concentration 1g crude drugs/mL is concentrated under reduced pressure to give in 50 DEG C, absolute pressure 0.01MPa combine decocting liquid； Be cooled in air 30 DEG C it is spare.
The Lowiaceae plant extraction concentrate obtains by the following method：
It is (1) 30 parts by weight of bark of Lowiaceae plant, 25 parts by weight of tree stem, 20 parts by weight of leaf are 4 hours dry in 60 DEG C, It crushes respectively, crosses 40 mesh sieve, be uniformly mixed so as to obtain mixture；
(2) mixture is immersed 40 DEG C, in the sodium hydrate aqueous solution of mass fraction 5%, dipping uses after 30 minutes 100 mesh filter-cloth filterings, take filter cake；Filter cake is washed with water to washing lotion to be in neutrality, is drained, extraction raw material is obtained；
(3) extraction is placed in raw material in refiner, the ethyl alcohol of volume fraction 50% is added, extraction raw material and ethyl alcohol Solid-to-liquid ratio is 1：2 (g/mL) are homogenized 3 times in 26 DEG C, every time extraction 5 minutes；Filtered through gauze, collection is used to carry after extracting solution is merged Take liquid A and filter cake；
(4) filter cake and water obtained step (3) is with solid-to-liquid ratio 1：6 (g/mL) mixings, in 50 DEG C ultrasonic power 300W, It is extracted 65 minutes under conditions of supersonic frequency 20kHz, collects extracting solution B；
(5) extracting solution A and extracting solution B are merged, vacuum distillation removes ethyl alcohol, obtains extracting solution；
(6) extracting solution is obtained into the Lowiaceae plant extraction concentrate by the ultrafiltration through membranes of molecular cut off 10KD.
The processing method of the black garlic extracting solution is：Fresh garlic is handled 24 hours in -20 DEG C of cryogenic freezings, is obtained pre- Treated garlic；Pretreated garlic is placed 40 days in the environment of 60 DEG C, humidity 70%, obtains black garlic；It weighs black Black garlic is broken into paste, the distilled water of 2 times of black garlic weight is added, in 60 DEG C in ultrasonic power 300W, supersonic frequency by garlic Ultrasonic extraction 30 minutes under conditions of 20kHz, obtain black garlic extracting solution.
Embodiment 5
Prostate cancer therapy liquid, including 10 parts by weight of Lowiaceae plant extraction concentrate, Chinese herbal medicine combine 30 weight of decocting liquid Part and 0.6 parts by weight of safflower pulp liquid.
Prepare prostate cancer therapy liquid：By Lowiaceae plant extraction concentrate, Chinese herbal medicine combination decocting liquid and safflower pulp liquid Be uniformly mixed to get.
The Chinese herbal medicine combination decocting liquid is prepared by following steps：
(1) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae are rinsed with water, remove surface silt, in 50 DEG C dry 4 hours, be cooled in air 30 DEG C it is spare；
(2) step (1) is weighed treated 10 parts by weight of Radix Astragali, 5 parts by weight of Chinese yew, 2 parts by weight of Radix Codonopsis, 2 weight of Rhizoma Atractylodis Macrocephalae Measure part, 5 parts by weight of Poria cocos, 8 parts by weight of matrimony vine, 3 parts by weight of Semen Cuscutae, 2 parts by weight of Chinese lobelia, 10 parts by weight of Radix Glycyrrhizae, the fruit of glossy privet 2 Parts by weight, 3 parts by weight of Herba Epimedii, 8 parts by weight of Cortex Phellodendri, 10 parts by weight of Radix Curcumae；
(3) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae crush respectively, cross 80 mesh sieve, are uniformly mixed so as to obtain herb powder；100 grams of herb powder is taken, is added water to cook, is decocted altogether It boils three times；1st decoction plus water 700mL, the 2nd time and the 3rd time decoction add water 300mL respectively, every time in 80 DEG C of decoctions when decoction It 30 minutes, after the completion of decoction, with filtered through gauze, collects the dregs of a decoction and decoction liquor, the dregs of a decoction is decocted next time again；Merging is decocted three times The decoction liquor boiled, the Chinese herbal medicine that concentration 1g crude drugs/mL is concentrated under reduced pressure to give in 50 DEG C, absolute pressure 0.01MPa combine decocting liquid； Be cooled in air 30 DEG C it is spare.
The Lowiaceae plant extraction concentrate obtains by the following method：
It is (1) 30 parts by weight of bark of Lowiaceae plant, 25 parts by weight of tree stem, 20 parts by weight of leaf are 4 hours dry in 60 DEG C, It crushes respectively, crosses 40 mesh sieve, be uniformly mixed so as to obtain mixture；
(2) mixture is immersed 40 DEG C, in the sodium hydrate aqueous solution of mass fraction 5%, dipping uses after 30 minutes 100 mesh filter-cloth filterings, take filter cake；Filter cake is washed with water to washing lotion to be in neutrality, is drained, extraction raw material is obtained；
(3) extraction is placed in raw material in refiner, the ethyl alcohol of volume fraction 50% is added, extraction raw material and ethyl alcohol Solid-to-liquid ratio is 1：2 (g/mL) are homogenized 3 times in 26 DEG C, every time extraction 5 minutes；Filtered through gauze, collection is used to carry after extracting solution is merged Take liquid A and filter cake；
(4) filter cake and water obtained step (3) is with solid-to-liquid ratio 1：6 (g/mL) mixings, in 50 DEG C ultrasonic power 300W, It is extracted 65 minutes under conditions of supersonic frequency 20kHz, collects extracting solution B；
(5) extracting solution A and extracting solution B are merged, vacuum distillation removes ethyl alcohol, obtains extracting solution；
(6) extracting solution is obtained into the Lowiaceae plant extraction concentrate by the ultrafiltration through membranes of molecular cut off 10KD.
The preparation of safflower pulp liquid：Fresh safflower fruit is selected, is crushed, is squeezed the juice 1 point with 2600 revs/min in juice extractor Clock collects juice；By juice filtered through gauze, filtrate is taken；Filtrate in 120 DEG C sterilizing 25 seconds after, be cooled to 30 DEG C it is spare.
Embodiment 6
Prostate cancer therapy liquid, including 10 parts by weight of Lowiaceae plant extraction concentrate, Chinese herbal medicine combine 30 weight of decocting liquid Part, 0.3 parts by weight of 0.3 parts by weight of black garlic extracting solution and safflower pulp liquid.
Prepare prostate cancer therapy liquid：By Lowiaceae plant extraction concentrate, Chinese herbal medicine combination decocting liquid, black garlic extracting solution and Safflower pulp liquid be uniformly mixed to get.
The Chinese herbal medicine combination decocting liquid is prepared by following steps：
(1) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae are rinsed with water, remove surface silt, in 50 DEG C dry 4 hours, be cooled in air 30 DEG C it is spare；
(2) step (1) is weighed treated 10 parts by weight of Radix Astragali, 5 parts by weight of Chinese yew, 2 parts by weight of Radix Codonopsis, 2 weight of Rhizoma Atractylodis Macrocephalae Measure part, 5 parts by weight of Poria cocos, 8 parts by weight of matrimony vine, 3 parts by weight of Semen Cuscutae, 2 parts by weight of Chinese lobelia, 10 parts by weight of Radix Glycyrrhizae, the fruit of glossy privet 2 Parts by weight, 3 parts by weight of Herba Epimedii, 8 parts by weight of Cortex Phellodendri, 10 parts by weight of Radix Curcumae；
(3) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, excessive sheep The leaves of pulse plants, Cortex Phellodendri, Radix Curcumae crush respectively, cross 80 mesh sieve, are uniformly mixed so as to obtain herb powder；100 grams of herb powder is taken, is added water to cook, is decocted altogether It boils three times；1st decoction plus water 700mL, the 2nd time and the 3rd time decoction add water 300mL respectively, every time in 80 DEG C of decoctions when decoction It 30 minutes, after the completion of decoction, with filtered through gauze, collects the dregs of a decoction and decoction liquor, the dregs of a decoction is decocted next time again；Merging is decocted three times The decoction liquor boiled, the Chinese herbal medicine that concentration 1g crude drugs/mL is concentrated under reduced pressure to give in 50 DEG C, absolute pressure 0.01MPa combine decocting liquid； Be cooled in air 30 DEG C it is spare.
The Lowiaceae plant extraction concentrate obtains by the following method：
It is (1) 30 parts by weight of bark of Lowiaceae plant, 25 parts by weight of tree stem, 20 parts by weight of leaf are 4 hours dry in 60 DEG C, It crushes respectively, crosses 40 mesh sieve, be uniformly mixed so as to obtain mixture；
(2) mixture is immersed 40 DEG C, in the sodium hydrate aqueous solution of mass fraction 5%, dipping uses after 30 minutes 100 mesh filter-cloth filterings, take filter cake；Filter cake is washed with water to washing lotion to be in neutrality, is drained, extraction raw material is obtained；
(3) extraction is placed in raw material in refiner, the ethyl alcohol of volume fraction 50% is added, extraction raw material and ethyl alcohol Solid-to-liquid ratio is 1：2 (g/mL) are homogenized 3 times in 26 DEG C, every time extraction 5 minutes；Filtered through gauze, collection is used to carry after extracting solution is merged Take liquid A and filter cake；
(4) filter cake and water obtained step (3) is with solid-to-liquid ratio 1：6 (g/mL) mixings, in 50 DEG C ultrasonic power 300W, It is extracted 65 minutes under conditions of supersonic frequency 20kHz, collects extracting solution B；
(5) extracting solution A and extracting solution B are merged, vacuum distillation removes ethyl alcohol, obtains extracting solution；
(6) extracting solution is obtained into the Lowiaceae plant extraction concentrate by the ultrafiltration through membranes of molecular cut off 10KD.
The processing method of the black garlic extracting solution is：Fresh garlic is handled 24 hours in -20 DEG C of cryogenic freezings, is obtained pre- Treated garlic；Pretreated garlic is placed 40 days in the environment of 60 DEG C, humidity 70%, obtains black garlic；It weighs black Black garlic is broken into paste, the distilled water of 2 times of black garlic weight is added, in 60 DEG C in ultrasonic power 300W, supersonic frequency by garlic Ultrasonic extraction 30 minutes under conditions of 20kHz, obtain black garlic extracting solution.
The preparation of safflower pulp liquid：Fresh safflower fruit is selected, is crushed, is squeezed the juice 1 point with 2600 revs/min in juice extractor Clock collects juice；By juice filtered through gauze, filtrate is taken；Filtrate in 120 DEG C sterilizing 25 seconds after, be cooled to 30 DEG C it is spare.
Test case 1
Experimental animal：Male SPF grades of rat, 180~220g of weight are purchased from Chengdu and reach large experimental animal Co., Ltd.It will Experimental animal is randomly divided into blank control group and 6 groups of experimental groups, every group 20.
Prepare Contained Serum：By the daily same time gavage prostate cancer therapy liquid of experimental animal 1 time, dosage 10mL/kg, Continuous 7 days.Rat Fast can't help water 12 hours before last time is administered.Etherization in 1 hour after last time is administered, eye socket take Blood detaches serum.The serum mixing of every group of experimental animal is placed in a pipe, 56 DEG C of inactivation antibody, using 0.22 μm of aperture Filter degerming, obtains Contained Serum.It is saved backup in -80 DEG C.
Prepare blank control serum：The same Contained Serum of preparation method, differs only in：Prostate cancer therapy liquid is replaced with Isometric distilled water.
The PBS solution of pH7.4 is prepared：It is accurate to weigh 8.5 grams of sodium chloride, 0.2 gram of potassium chloride, 2.85 grams of disodium hydrogen phosphates, 0.22 gram of potassium dihydrogen phosphate, stirring adjust pH to pH7.4, are settled to 1L using distilled water, high pressure sterilization is set to being completely dissolved It is saved backup in 4 DEG C.
Cell culture：Human Prostate Cancer PC-3 Cell Line (being purchased from the Shanghai bio tech ltd Bai Li) is inoculated in containing 10% In the DMEM complete mediums of fetal calf serum, 100U/mL penicillin and 100U/mL streptomysins, it is placed in 37 DEG C, 5%CO2CO2Training Routine culture in supporting replaces a subculture in 2~3 days, with 0.25% trypsin digestion, biography when cell grows to 80~90 Generation, propagated cell.
The flow cytomery cell cycle：It is dense with DMEM culture solutions adjustment cell by the PC-3 cells of exponential phase Degree is 4 × 106A/mL is inoculated in 48 well culture plates, per 400 μ L of hole.Culture plate is moved into CO2In culture, in 37 DEG C, 5%CO2 Under conditions of cultivate 24 hours.After 24 hours, the Contained Serum of concentration 10% is added, the blank of isoconcentration is added in blank control group Serum continues to cultivate cell 36 hours.Respectively collect each group cell, be added 5mL PBS solutions washing, with 1000 revs/min from The heart 5 minutes, abandons supernatant；70% ethyl alcohol of 2mL is injected with syringe, is placed in 4 DEG C of refrigerators and fixes 4 hours, with 1000 revs/min Zhongli's heart 5 minutes, abandons ethyl alcohol；After being cleaned using PBS solution, the PI dyes containing 50 μ g/L PI and 10mg/mL RNAase are added Liquid, 37 DEG C are protected from light incubation 30 minutes, and through 100 mesh nylon net filters, the cell cycle is measured using flow cytometer.
Data difference (P ＜ 0.05) between experimental group is statistically significant.
Specific test result is shown in Table 1.
Table 1：Influence of the prostate cancer therapy liquid to PC-3 cell cycles each phase
G0/G1 S G2/M
Blank control group 58.51 25.55 15.94
Embodiment 1 59.17 20.13 20.70
Embodiment 2 58.09 19.40 22.51
Embodiment 3 57.18 17.59 25.23
Embodiment 4 56.96 14.85 28.19
Embodiment 5 57.03 15.92 27.05
Embodiment 6 57.50 13.93 28.57
As known from Table 1, the medicine of liquid containing prostate cancer therapy serum handled PC-3 cells after 36 hours, and G2/M phase cell proportions go out Now increase, S phase cell proportions are substantially reduced, and G0/G1 phases cell proportion, without significant change, this illustrates prostate cancer therapy liquid pair The proliferation of prostate gland cancer cell PC-3 cells plays inhibiting effect.
Test case 2
The preparation of MTT solution：It accurately weighs 50mgMIT to be dissolved in 10mLPBS solution, stands overnight, use 0.22 μ of aperture The membrane filtration of m, sealed membrane sealing, is placed in 4 DEG C and is kept in dark place.
Mtt assay detects the Proliferation Ability of cell：The PC-3 cells of logarithmic growth phase, 1 is configured to DMEM culture solutions × 105The cell suspension of a/mL, with every hole 1 × 104A cell inoculation is in 96 orifice plate culture plates.Culture plate is moved into CO2Culture In, in 37 DEG C, 5%CO2Under conditions of cultivate 24 hours.After 24 hours, the Contained Serum of various concentration is added in experimental group.It will training Foster plate, which is placed in incubator, continues culture 36 hours, and the 20 μ L of MTT solution of 5mg/mL are then added per hole, continue culture 4 hours. Careful inhale abandons supernatant in hole, and 200 μ LDMSO, oscillation mixing 10 minutes are then added per hole.It is measured at 570nm using microplate reader OD values.Negative control group only adds the DMSO of isoconcentration, not increase serum；The blank serum of isoconcentration is added in blank control group.
5 parallel holes of every group of setting, calculate the inhibiting rate of cell：The inhibiting rate of cell=(blank control group OD values-experiment Group OD values)/(blank control group OD values-negative control group OD values) × 100%.Using cell inhibitory rate as ordinate, with drug containing blood Clear a concentration of abscissa, draws the growth curve of cell, calculates half-inhibition concentration (IC of the Contained Serum to cell50Value).
Data difference (P ＜ 0.05) between experimental group is statistically significant.
Specific test result is shown in Table 2.
Table 2：Prostate cancer therapy liquid is to IC50The influence of value
IC50Value refers to that the quantity for the cell survived after medication reduces commonly required drug concentration.IC50It is worth smaller, explanation The drug effect of prostate cancer therapy liquid is stronger, more notable to the inhibiting effect of prostate cancer cancer cell.From table 2 it can be seen that implementing The drug effect of example 2~3 is better than embodiment 1, and reason may be embodiment 2~3 when extracting Lowiaceae plant extraction liquid, first by any of several broadleaf plants Bark, leaf, the rhizome of section plant are impregnated with sig water, remove the fatty wax on surface so that active constituent is easy to release It puts；Further, embodiment 3 increases the processing step of homogenate extraction, to substances such as terpenol, flavones in Lowiaceae plant Bring up again and take, so as to improve with Lowiaceae plant extraction liquid with the drug effect of the prostate cancer therapy liquid of main active.
Test case 3
Patients with prostate cancer is recruited, the age, more than half a year, patient met the course of disease between 30~60 years old《New Chinese medicine Treat the guideline of clinical investigations of chronic prostatitis (non-specificity)》In diagnostic criteria.
Clinical symptoms foundation《TCM surgery disease Standardization of diagnosis and curative effect》Judgement is as follows：Paruria, frequent micturition is unsmooth, urethra Scorching hot, urine is just or the last pain of urine, pain emit to glans penis, perineum, groin, and testis has falling inflation sense of discomfort, urinates Posterior urethral Mouth has white secretion to overflow, once in a while concurrent sex dysfunction, including hyposexuality, premature ejaculation, painful ejaculation, decrease of erecing and sun Impotence.
Instructions of taking：Oral prostate cancer therapy liquid medicine, twice daily, once in the morning and once at night, each 10mL, treatment in 20 days Journey.
Effectively, effective and invalid evaluation criteria is as follows：
It is effective：Clinical symptoms all disappear；
Effectively：Clinical symptom relief, the state of an illness are controlled；
In vain：No significant improvement or aggravated in clinical symptoms.
Specific test result is shown in Table 3.
Table 3：Influence of the prostate cancer therapy liquid to curative effect
From table 3 it can be seen that taking the patient of the prostate cancer therapy liquid of the extracting solution containing black garlic, black garlic is free of compared to taking The patient of extracting solution, efficient and obvious effective rate increase.The Alkaloid of black garlic has very strong removing Both peroxyl radical Ability, and the present invention pre-processes garlic before preparing black garlic, promotes the generation of reduced sugar, accelerates Maillard reaction Progress, reduce bitter substance simultaneously, have it is anti-oxidant, improve hemorheology etc. to the advantageous effect of human body.
Test case 4
The bacteriostatic activity of the prostate cancer therapy liquid of embodiment 6 is measured.
The fungistatic effect of prostate cancer therapy liquid is measured using Odontothrips loti：Take concentration 2.0 × 108The bacteria suspension of CFU/mL 0.1mL is coated on solidification tablet, and each tablet is put into 2 sterilizing Oxford cups later, wherein 1 is parallel, 1 is used as pair According to (as a contrast with sterile water).It pipettes prostate cancer therapy liquid with liquid-transfering gun respectively to be added in Oxford cup, 37 DEG C of cultures of bacterium 24 hours, 28 DEG C of aspergillus niger was cultivated 48 hours, and Oxford cup is removed, and measures antibacterial circle diameter.Every group parallel 3 times, take its average value.
The UV stable of prostate cancer therapy liquid is measured：By prostate cancer therapy liquid power 20W purple It is handled 30 minutes under outer lamp, then measures the size of inhibition zone.
The ph stability of prostate cancer therapy liquid is measured：With the hydrochloric acid and mass fraction of mass fraction 10% The pH of prostate cancer therapy liquid is adjusted to 5,9 by 10% sodium hydrate aqueous solution respectively, then measures the size of its inhibition zone.
Specific test result is shown in Table 4.
Table 4：Prostate cancer therapy liquid antibacterial circle diameter
From table 3 it can be seen that prostate cancer therapy liquid of the present invention has natural fungistatic effect, and biocidal property will not It is influenced by hot, ultraviolet and acid or alkali environment, there is good ultraviolet stability and ph stability.
Concrete application example
Patient's basic document：Chen, man, 61 years old, the multiple Bone tumour of prostate cancer.
In July, 2015, Chen are diagnosed as prostate cancer.It has done the operation of bilateral testes castration under the suggestion of doctor. But in May, 2016 check shows disease relapse, and multiple Bone tumour occurs.Due to there is Bone tumour, patient not only get over by pain Carry out more serious, and difficulty in walking.After by the agency of, patient takes prostate cancer therapy liquid of the present invention, and after 8 courses for the treatment of, patient is anti- " being previously required to walk by cane, oneself can normally walk now " is reflected, and pain is eased, diet, sleep, spirit are It takes an evident turn for the better.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification should all belong to the covering scope of the present invention.

Claims (10)

1. prostate cancer therapy liquid, which is characterized in that combine decocting liquid including Lowiaceae plant extraction concentrate and Chinese herbal medicine.
2. prostate cancer therapy liquid as described in claim 1, which is characterized in that including Lowiaceae plant extraction concentrate 5~15 Parts by weight and Chinese herbal medicine combine 10~30 parts by weight of decocting liquid；
The Chinese herbal medicine combination decocting liquid is prepared by following steps：
(1) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, Herba Epimedii, Huang Cypress, Radix Curcumae are rinsed with water, and remove surface silt, and cold standby is set in drying；
(2) step (1) is weighed treated 8~15 parts by weight of Radix Astragali, 3~7 parts by weight of Chinese yew, 2~4 parts by weight of Radix Codonopsis, in vain 2~4 parts by weight of art, 5~10 parts by weight of Poria cocos, 6~10 parts by weight of matrimony vine, 3~7 parts by weight of Semen Cuscutae, 2~4 weight of Chinese lobelia Part, 5~15 parts by weight of Radix Glycyrrhizae, 2~5 parts by weight of the fruit of glossy privet, 3~7 parts by weight of Herba Epimedii, 8~15 parts by weight of Cortex Phellodendri, Radix Curcumae 8~ 15 parts by weight；
(3) by Radix Astragali, Chinese yew, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, matrimony vine, Semen Cuscutae, Chinese lobelia, Radix Glycyrrhizae, the fruit of glossy privet, Herba Epimedii, Huang Cypress, Radix Curcumae crush respectively, and sieving is uniformly mixed so as to obtain herb powder；100~150 grams of herb powder is taken, adds water to carry out 2~3 times and decocts It boils；600~800mL of 1st decoction plus water, the 2nd~3 decoction adds 300~400mL of water respectively, every time in 70~80 when decoction It DEG C decocts 30~40 minutes, after the completion of decoction, filtering is collected the dregs of a decoction and decoction liquor, the dregs of a decoction and decocted next time again；Merge every The decoction liquor of secondary decoction is concentrated to give the Chinese herbal medicine combination decocting liquid of 1~1.2g of concentration crude drugs/mL；Set cold standby.
3. prostate cancer therapy liquid as claimed in claim 2, which is characterized in that the Lowiaceae plant extraction concentrate by with Lower method obtains：
(1) 20~50 parts by weight of bark of Lowiaceae plant, 20~50 parts by weight of tree stem, 20~50 parts by weight of leaf are dried, point It does not crush, is sieved, is uniformly mixed so as to obtain mixture；
(2) by mixture and extraction solvent with solid-to-liquid ratio 1：(1~10) (g/mL) mixing, in 50~60 DEG C of ultrasonic extractions 40~ 100 minutes, extracting solution is collected, the Lowiaceae plant extraction concentrate is obtained.
4. prostate cancer therapy liquid as claimed in claim 2, which is characterized in that the Lowiaceae plant extraction concentrate by with Lower method obtains：
(1) 20~50 parts by weight of bark of Lowiaceae plant, 20~50 parts by weight of tree stem, 20~50 parts by weight of leaf are dried, point It does not crush, is sieved, is uniformly mixed so as to obtain mixture；
(2) mixture is immersed 30~40 DEG C, in the sodium hydrate aqueous solution of mass fraction 5~20%, impregnated 30~40 minutes Afterwards, it filters, takes filter cake；Filter cake is washed with water to washing lotion to be in neutrality, is drained, extraction raw material is obtained；
(3) by extraction raw material and extraction solvent with solid-to-liquid ratio 1：(1~10) (g/mL) mixing, in 50~60 DEG C of ultrasonic extractions 40~100 minutes, collect extracting solution；
(4) extracting solution is obtained into the Lowiaceae plant extraction concentrate by the ultrafiltration through membranes of 10~100KD of molecular cut off.
5. prostate cancer therapy liquid as claimed in claim 2, which is characterized in that the Lowiaceae plant extraction concentrate by with Lower method obtains：
(1) 20~50 parts by weight of bark of Lowiaceae plant, 20~50 parts by weight of tree stem, 20~50 parts by weight of leaf are dried, point It does not crush, is sieved, is uniformly mixed so as to obtain mixture；
(2) mixture is immersed 30~40 DEG C, in the sodium hydroxide solution of mass fraction 5~20%, dipping 30~after forty minutes, Filtering, Washing of Filter Cake to neutrality drains, obtains extraction raw material；
(3) extraction is placed in raw material in refiner, the ethyl alcohol of volume fraction 50~80% is added, extraction raw material and ethyl alcohol Solid-to-liquid ratio is 1：(2~5) (g/mL) is extracted 3~4 times in 20~30 DEG C of homogenate, is extracted 3~5 minutes every time；Extracting solution is merged Filtered through gauze is used afterwards, collects extracting solution A and filter cake；
(4) filter cake and extraction solvent obtained step (3) is with solid-to-liquid ratio 1：(1~10) (g/mL) mixing, in 50~60 DEG C Ultrasonic extraction 40~100 minutes collects extracting solution B；
(5) extracting solution A and extracting solution B are merged, vacuum distillation removes ethyl alcohol, obtains extracting solution；
(6) extracting solution is obtained into the Lowiaceae plant extraction concentrate by the ultrafiltration through membranes of 10~100KD of molecular cut off.
6. the prostate cancer therapy liquid as described in any one of claim 3~5, which is characterized in that the Extraction solvent is water Or the sodium-chloride water solution of 3-10%, 35-55 DEG C of mass fraction.
7. prostate cancer therapy liquid as claimed in claim 2, which is characterized in that the prostate cancer therapy liquid further includes 0.1 ~1 parts by weight black garlic extracting solution and/or safflower pulp liquid.
8. prostate cancer therapy liquid as claimed in claim 7, which is characterized in that the processing method of the black garlic extracting solution is： Fresh garlic is pre-processed；Pretreated garlic is placed 20~45 days in the environment of 60~80 DEG C, humidity 40~70%, Obtain black garlic；Black garlic is weighed, black garlic is broken into paste, the distilled water of 1~2 times of black garlic weight is added, in 60~70 DEG C of ultrasounds Extraction 20~40 minutes, obtains black garlic extracting solution；
The processing method of the safflower pulp liquid is：Fresh safflower fruit is selected, is crushed, squeezes the juice, collects juice；By juice yarn Cloth filters, and takes filtrate；Filtrate sterilizes, and sets cold standby.
9. prostate cancer therapy liquid as claimed in claim 8, which is characterized in that the pretreatment mode of the fresh garlic is： Fresh garlic is handled 24~36 hours in -20~-18 DEG C of cryogenic freezings, or fresh garlic is high in 90~100 DEG C of water Warm water boils processing 3~5 hours.
10. the preparation method of prostate cancer therapy liquid as claimed in claim 2, which is characterized in that include the following steps：By any of several broadleaf plants Section's plant extraction concentrate and Chinese herbal medicine combination decocting liquid be uniformly mixed to get.
CN201810526628.3A 2018-05-22 2018-05-22 Prostate cancer therapy liquid and preparation method thereof Withdrawn CN108379521A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108578557A (en) * 2018-07-26 2018-09-28 广西中绿医药科技有限公司 Additive and its application process for prostate cancer healing liquid
CN108671202A (en) * 2018-07-26 2018-10-19 广西中绿医药科技有限公司 Prostate cancer healing liquid and preparation method thereof
CN112274579A (en) * 2020-10-14 2021-01-29 广西天缘绿生物科技有限公司 Blood waste fast removing banana plant extract

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108578557A (en) * 2018-07-26 2018-09-28 广西中绿医药科技有限公司 Additive and its application process for prostate cancer healing liquid
CN108671202A (en) * 2018-07-26 2018-10-19 广西中绿医药科技有限公司 Prostate cancer healing liquid and preparation method thereof
CN112274579A (en) * 2020-10-14 2021-01-29 广西天缘绿生物科技有限公司 Blood waste fast removing banana plant extract

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