CN112156113A - Method for cultivating bezoar in ox gall by Chinese herbal medicine induction - Google Patents

Abstract

A method for cultivating bezoar in ox gall by using Chinese herbal medicine induction relates to a cultivation method of Chinese medicinal therapeutic medicines, in particular to a method for artificially cultivating bezoar. The specific operation method comprises the following steps: selecting cattle: the breed and the male and female are not limited, but the cattle losing the reproduction or the work ability can also be selected, and the local breed yellow cattle is preferably selected, the older is the better; the traditional Chinese medicine is taken by feeding, and the traditional Chinese medicine formula comprises 30 parts of lucid ganoderma, fructus cannabis, pine nut kernel, cortex acanthopanacis, radix angelicae pubescentis, rhizoma atractylodis, mangnolia officinalis, poria cocos, polyporus umbellatus, spina date seed, semen boitae, angelica sinensis, prepared rehmannia root, white chrysanthemum, polygonum multiflorum, eucommia ulmoides, astragalus mongholicus, rhizoma atractylodis macrocephalae, Chinese yam, lily and radix ophiopogonis respectively; as a result of the application, the cow yellow production per head of cow is about 100 g, and the bilirubin content in cow-bezoar is above 70%.

Description

Method for cultivating bezoar in ox gall by Chinese herbal medicine induction

Technical Field

The invention relates to a cultivation method of a Chinese medicinal treatment medicine, in particular to an artificial bezoar cultivation method.

Background

Calculus bovis is a dried body of gallstone of animals of the family Bovidae. Is a granular substance or a block substance formed by the inflammation of the biliary tract of a sick cattle or the elimination of bile, and the precipitation and coagulation of cholesterol, calcium salt and cholesterol in the bile. If calculus bovis is found in the old cattle, taking out calculus bovis, removing external film, and drying in the shade. The amount of the natural bezoar is small, the natural occurrence rate of the natural bezoar is only 0.921 percent, and the medicine source is deficient. The annual bezoar demand of China is about 5 tons, but the self-produced natural bezoar is less than 1 ton. The artificial bezoar has inferior efficacy, and the relevant substances extracted from the pig bile can be prepared into the artificial bezoar with the price less than 0.5 percent of the natural bezoar. Moreover, the efficacy of calculus bovis factitius cannot completely replace natural calculus bovis. The bezoar cultivated in the cattle body has good efficacy, but is difficult to grow like pearl cultivation, and foreign matters are embedded in the cattle body at present to promote the growth of calculus. In the highest peak of China, only 1000 cattle are subjected to the operation. But the success rate is not high and the yield is low.

Disclosure of Invention

The invention aims to utilize the Chinese herbal medicine oral administration of cattle to condition cattle gallbladder to quickly form gallstone, thereby improving the yield of bezoar.

The technical scheme of the invention is as follows: a method for cultivating calculus bovis in fel bovis Seu Bubali by inducing Chinese herbal medicines comprises the following steps:

1. cattle selection for cultivating bezoar: the breed and the male and female are not limited, but the cattle losing the reproduction or working ability can also be selected, in order to ensure that the bezoar has good quality and high yield, the local breed cattle is selected, and the larger the age is, the better the breed is;

2. the traditional Chinese medicine is taken by feeding, and the traditional Chinese medicine formula comprises 30 parts of lucid ganoderma, fructus cannabis, pine nut kernel, cortex acanthopanacis, radix angelicae pubescentis, rhizoma atractylodis, mangnolia officinalis, poria cocos, polyporus umbellatus, spina date seed, semen boitae, angelica sinensis, prepared rehmannia root, white chrysanthemum, polygonum multiflorum, eucommia ulmoides, astragalus mongholicus, rhizoma atractylodis macrocephalae, Chinese yam, lily and radix ophiopogonis respectively;

3. preparing the medicaments according to the formula, weighing corresponding fractions, respectively pulverizing into powder, and uniformly mixing for later use; when in use, each cow is weighed with the dose of 100 g/Qianjin every time, mixed with brown sugar or edible vegetable oil or mixed with brown sugar and edible vegetable oil, the medicine is filled in the morning with empty stomach, once a day, and continuously fed for 15 days, and bezoar begins to form in the gallbladder; continuously culturing for about six months, killing cattle and taking out calculus bovis.

The medicinal properties of the components of the formula are as follows:

the ganoderma lucidum powder has the following functions: firstly, the development induced allergy is caused, especially the immune system of old cattle is reduced, and the ganoderma lucidum powder interferes with normal metabolism. And secondly, the ganoderma lucidum powder can generate strong effect on old cows. And thirdly, for serious liver and kidney deficiency, particularly liver cirrhosis or renal failure, the application of the ganoderma lucidum powder can cause obvious organ failure aggravation. It is sweet in flavor and neutral in nature; it enters heart, lung, liver and kidney meridians.

Hemp seed, fructus Cannabis, can cause some digestive tract disorders, such as nausea, vomiting, diarrhea, numbness in the extremities. Overdosing may also have an effect on the kidneys. Because the fructus cannabis can stimulate the intestinal tract, the motility of the gastrointestinal tract can be promoted, and the function of relaxing the bowels can be achieved. Therefore, patients with frequent diarrhea, such as those with weak spleen and stomach, may have symptoms of diarrhea aggravated due to inability to form stool. .

The semen Pini kernel is seed kernel of Pinus koraiensis of Pinaceae, and has side effect 1, high oil content, and rapid heat supply. The excessive consumption of the food can accumulate excessive fat, and the atherosclerosis, even the hypertension and the heart disease can be caused after the long-term consumption. 2. Pine nuts can cause taste disturbances in humans, produce bitter and astringent or metallic tastes, and typically produce symptoms 1-3 days after consumption, and last for days or weeks. Semen Pini kernel contains a large amount of unsaturated fatty acid, has effects of strengthening body constitution, invigorating kidney, invigorating qi, nourishing blood, moistening intestine, and nourishing body, and is especially suitable for senile asthenia, lumbago, constipation, giddiness, and infantile growth and development retardation.

Acanthopanacis cortex is effective in the development of insomnia and allergy, even anxiety and depressed mood, or precordial pain and headache symptoms, even leading to the development of blood pressure elevation and dyspnea. Promoting hyperactivity of fire due to yin deficiency.

Pubescent angelica root, name of Chinese traditional medicine. Is dried root of Angelica gigas nakai of Umbelliferae. Has the effects of dispelling pathogenic wind, removing dampness, relieving arthralgia and relieving pain. Can be used for treating arthralgia due to wind-cold-dampness, lumbago, gonalgia, headache due to wind-cold-yin deficiency, and headache due to wind-cold-dampness. It should be used with cautions for yin deficiency and blood dryness, and not used for general pain due to qi and blood deficiency and flaccidity of lower body due to yin deficiency. For all kinds of deficient wind, salty is not suitable for Du Huo.

Rhizoma atractylodis, the name of traditional Chinese medicine. Is dried rhizome of Atractylodes lancea or Atractylodes lancea. Dry dampness and invigorate spleen, dispel wind and cold, improve vision. Compendium of materia Medica: peach, plum, sparrow meat, woad and black carp are avoided.

Magnolia bark, name of traditional Chinese medicine. Is dried bark, root bark and branch bark of Magnolia officinalis or Magnolia obovata Thunb of Magnoliaceae. Dry dampness and resolve phlegm, descend qi and remove fullness. Can be used for treating damp stagnation, abdominal distention, vomiting, diarrhea, food stagnation, qi stagnation, abdominal distention, constipation, phlegm retention, asthma, and cough.

Tuckahoe, name of traditional Chinese medicine. Is dried sclerotium of Poria cocos (Schw.) wolf of Polyporaceae. The functions of promoting diuresis, eliminating dampness, invigorating spleen and calming heart. It can be used for treating edema, oliguria, palpitation due to phlegm and fluid retention, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia. It is contraindicated for yin deficiency with effulgent fire.

Polyporus umbellatus, medicinal fungus of Grifola of Polyporaceae of Aphyllophorales. The fruit body is large or very large, the flesh has stems and multiple branches, the tail end of the mushroom cap is rounded and white to light brown, the fruit body is edible when being tender, and the taste is very delicious. The underground sclerotium is black and various in shape, is a famous traditional Chinese medicine, and has the effects of promoting urination and treating edema. Contains Polyporus polysaccharide, and is used for resisting cancer.

Semen Ziziphi Spinosae is seed of Ziziphus jujube Mill of Rhamnaceae. Harvesting in autumn when the fruit is mature, soaking the fruit for a night, removing pulp, taking out, grinding the kernel with stone roller, taking out the seed, and drying in the sun. Enter heart, spleen, liver and gallbladder meridians. The main functional indications are as follows: nourish liver, calm heart, induce tranquilization, arrest sweating. It is indicated for restlessness and insomnia, palpitation, polydipsia, and sweating due to debility.

Baizi ren, name of Chinese medicine. Is dried mature kernel of Platycladus orientalis of Cupressaceae. Harvesting mature seeds in autumn and winter, drying in the sun, removing seed coats, and collecting kernels. Has the effects of nourishing heart, tranquilizing mind, loosening bowel to relieve constipation, and arresting sweating. Can be used for treating yin and blood deficiency, vexation, insomnia, palpitation, constipation due to intestinal dryness, and night sweat due to yin deficiency. Has the effects of nourishing heart, tranquilizing mind, loosening bowel to relieve constipation, and arresting sweating. The main treatment is as follows: can be used for treating yin and blood deficiency, vexation, insomnia, palpitation, constipation due to intestinal dryness, and night sweat due to yin deficiency. Cautious clothes for loose stool and profuse sputum

The Chinese angelica is also called dry angelica, Qina, western angelica, Min angelica, golden angelica, culvert angelica tail, angelica yeast and angelica sinensis, and the root of the Chinese angelica can be used as a medicine, and is one of the most commonly used Chinese medicines. Has effects in nourishing blood, regulating menstruation, relieving pain, moistening dryness, smoothing intestine, resisting cancer, resisting aging, and enhancing immunity.

The prepared rehmannia root has the main functions of nourishing yin and enriching blood. It is indicated for deficiency of yin and blood, atrophy and weakness of waist and knees, fatigue, cough, steaming bone, seminal emission, metrorrhagia, menoxenia, diabetes, scanty urine, deafness and blurred vision. Nourishing kidney water, sealing and filling bone marrow, promoting blood circulation, invigorating yin, improving hearing and eyesight, blackening hair, and blackening beard. Can also tonify spleen yin, stop chronic diarrhea, treat overstrain wind arthralgia, yin deficiency fever, dry cough with phlegm, short breath, dyspnea, deficiency of stomach qi and discouraging, acne syndrome with no pus due to heart deficiency, shin and thigh ache after illness, postpartum umbilical and abdominal pain, cold syndrome with yin deficiency, no sweat and constipation, various bleeding, all liver and kidney yin deficiency, deficiency and all diseases, and is the main medicine for strengthening water.

The feverfew belongs to Compositae and perennial flowers. The chrysanthemum contains effective chemical components, such as adenine, choline, stachydrine, etc., and the volatile oil extracted from chrysanthemum contains chrysanthemum ketone, borneol acetate, etc. Can enhance capillary resistance, inhibit capillary permeability, and has antiinflammatory and body constitution strengthening effects.

The fleece-flower root is also called polygonum multiflorum, glaucescent fissistigma root, tuber fleeceflower stem and the like. The tuber root is used as a medicine for soothing the nerves, nourishing the blood, activating the collaterals, detoxifying (preventing malaria) and eliminating carbuncle, and the prepared fleece flower root can tonify the essence and blood, blacken beard and hair, strengthen the bones and muscles and tonify the liver and kidney, and is a common precious and thin Chinese medicinal material.

Eucommia ulmoides, also known as Bakelite, is a plant of the family Eucommiaceae. The eucommia ulmoides has few free amino acids, contains a small amount of protein and 15 mineral elements, and is medicinal eucommia ulmoides, namely dried bark of eucommia ulmoides of the family eucommia ulmoides, which is a famous and precious Chinese medicinal material. It is sweet in taste and warm in nature. Has effects in tonifying liver and kidney, strengthening muscle and bone, regulating Chong and ren meridians, and consolidating channels.

Astragalus membranaceus, also known as astragalus membranaceus. Has effects in enhancing immunity, protecting liver, promoting urination, resisting aging, resisting stress, lowering blood pressure, and inhibiting bacteria. But excessive superficial pathogenic factors, qi stagnation and dampness obstruction, food stagnation, initial carbuncle and deep-rooted carbuncle or excessive heat-toxicity after ulceration, and yin deficiency and yang hyperactivity are prohibited.

Rhizoma Atractylodis Macrocephalae and Oxalid floating thistle, Atractylodes chinensis, Thunberg, Populus raft, Wushu, Yeshu, Atractylodes lancea, etc., belonging to Compositae and Atractylodes perennial herb. In cool climate, the rhizome is used as medicine, and has multiple medicinal functions. The rhizoma atractylodis macrocephalae has the effects of strengthening spleen and tonifying qi, eliminating dampness and promoting diuresis, suppressing sweating and preventing miscarriage.

Rhizoma Dioscoreae and rhizoma Dioscoreae are plants of Dioscorea of Dioscoreaceae, and are wound with grass vine. It can tonify spleen and stomach deficiency, and is used for treating qi deficiency, asthenia, dyspepsia, spermatorrhea, enuresis, and innominate toxic swelling. The medicine is that the tuber of Chinese yam is the common Chinese medicine 'Huaishan', the root can be used as medicine, is sweet, warm and flat and has no toxicity. Mainly tonify deficiency and win, remove cold and heat evil qi, tonify middle-jiao, benefit strength, strengthen muscles, strengthen yin, wind wandering to the head and face, dizziness and dizzy, descend qi, stop lumbago, treat consumptive disease and thin, fill five internal organs, and remove dysphoria with smothery sensation. Tonify the five consumptive diseases and seven injuries, remove cold wind, calm the heart, tranquilize mind, tonify heart qi deficiency and open up the heart hole for recording many events. And (6) screening. Strengthen tendons and bones, induce emission and induce amnesia. The swelling and toxicity of raw pounded plaster can be dissipated.

The lily is a perennial herb bulbous plant of lily of Liliaceae, and the fresh lily has the effects of nourishing heart, tranquilizing mind, moistening lung and relieving cough in traditional Chinese medicine. Nourishing yin and moistening lung; clearing away heart-fire and tranquilizing. Chronic cough due to yin deficiency; blood in sputum; late stage of febrile disease; restlessness, pavor, insomnia, dreaminess and absentmindedness due to residual heat or emotional disorder; abscess and swelling; eczema

Ophiopogon japonicus of Liliaceae, Ophiopogon genus of perennial evergreen herb, radix Ophiopogonis is Chinese medicinal material with effects of promoting fluid production, quenching thirst, moistening lung and relieving cough. Radix Ophiopogonis has effects of promoting recovery of islet cell function, increasing hepatic glycogen, and reducing blood glucose. Some diabetics have deficiency of both qi and yin, so when drinking the radix ophiopogonis water, the radix codonopsis can be matched with a little radix codonopsis, and the effect of tonifying qi can be achieved.

The principle of action of the components in the formula is as follows:

the prescription utilizes Chinese herbal medicines to increase the phospholipase in the ox gall, phospholipid is converted into lysolecithin and free fatty acid under the hydrolysis action of the phospholipase in the ox gall, and the combined bile salt in the gall is converted into free bile salt under the action of bacterial enzyme, so that the combined bile salt in the gall is reduced, and the concentration of free calcium in the gall is increased due to the reduction of micelles and vesicles of the bile salt-phospholipid which dissolves free calcium and is not combined with bilirubin. At this time, the bile contains a large increase in micelles and vesicles of bile salt-phospholipid which do not bind bilirubin, and the concentration of free calcium in the bile increases. Resulting in a large increase of calcium in the bile combined with unbound bilirubin to form a precipitate and calculus, forming calculus bovis in the traditional Chinese medicine.

Specifically, the method comprises the following steps: the ganoderma lucidum, the cortex acanthopanacis, the spina date seed, the angelica sinensis, the polygonum multiflorum, the eucommia ulmoides, the mangnolia officinalis and the radix angelicae pubescentis can increase the absorption of protein, fat and confectionery, change the level of endocrine hormone and cause bovine hypercholesterolemia. The sick cattle are weaker and the bacterial infection is accelerated to form primary bile duct calculi.

The fructus cannabis, pine nut kernel, tuckahoe, grifola, lily and radix ophiopogonis can reduce cholesterol in blood plasma, lead the cholesterol in the blood plasma to be discharged from the liver into the cholesterol and increase the cholesterol content in the bile.

Thirdly, the Chinese atractylodes rhizome, platycladi seed, white chrysanthemum, Chinese yam, prepared rehmannia root, astragalus root and white atractylodes rhizome can damage the emptying function of a gall bladder, block the circulation of liver and intestine, lead the gall to be silted up, lead bile salt to be lost and increased through excrement to become supersaturated cholesterol bile, lead the concentration of cholesterol or calcium in the bile to be too high, lead the bile to be easy to form crystals, precipitate, aggregate and fuse to form calculus.

The method for cultivating the bezoar is characterized in that the bezoar is cultivated according to the combination of a bezoar forming mechanism and a modern biological principle, the constitution of a cow is weakened, the immunity of a gall bladder is reduced, and the biological environment in the gall bladder is changed. The results of comparative analysis and experimental research on various indexes of the cultured bezoar and the natural bezoar in the aspect of pharmacological efficacy have no significant difference. The experimental result shows that the cow yellow production of each cow is about 100 g, the bilirubin content of the cow is more than 70%, and the method is successfully applied to 36 clinical experiments.

Detailed Description

The clinical experiment proves that the invention comprises the following steps: selecting two aged cattle with the weight of 200-300KG and local yellow cattle in Jiangxi, Fujian and northeast Jilin each year from 2014 to 2020, continuously drenching the formula for 15 days, combining captive breeding and stocking, and slaughtering after six months to obtain about 100 g of bezoar.

The inspection shows that the content of bilirubin in the bezoar is more than 70%, and the quality of the bezoar reaches the national natural medicinal standard.

Claims (2)

1. A method for cultivating bezoar in ox gall by using Chinese herbal medicine induction is characterized in that: the specific operation method comprises the following steps:

1) cattle selection for cultivating bezoar: the variety and the male and female are not limited, and the local variety of cattle is selected, wherein the older the cattle is, the better the cattle is;

2) the traditional Chinese medicine is taken by feeding, and the traditional Chinese medicine formula comprises 30 parts of lucid ganoderma, fructus cannabis, pine nut kernel, cortex acanthopanacis, radix angelicae pubescentis, rhizoma atractylodis, mangnolia officinalis, poria cocos, polyporus umbellatus, spina date seed, semen boitae, angelica sinensis, prepared rehmannia root, white chrysanthemum, polygonum multiflorum, eucommia ulmoides, astragalus mongholicus, rhizoma atractylodis macrocephalae, Chinese yam, lily and radix ophiopogonis respectively;

3) preparing the medicaments according to the formula, weighing corresponding fractions, respectively pulverizing into powder, and uniformly mixing for later use; when in use, each cow is weighed with the dose of 100 g/Qianjin every time, mixed with brown sugar or edible vegetable oil or mixed with brown sugar and edible vegetable oil, the medicine is filled in the morning with empty stomach, once a day, and continuously fed for 15 days, and bezoar begins to form in the gallbladder; continuously culturing for about six months, killing cattle and taking out calculus bovis.

2. The method for cultivating calculus bovis in the gallbladder of cattle induced by Chinese herbal medicine as claimed in claim 1, wherein the cattle for cultivating calculus bovis are selected from: cattle that lose reproduction or work capacity.