CN112717047A

CN112717047A - Medicine for treating osteoporosis and treatment method thereof

Info

Publication number: CN112717047A
Application number: CN202110252864.2A
Authority: CN
Inventors: 韩鹏勃; 蒋锋
Original assignee: Second Affiliated Hospital of Xian Medical University
Current assignee: Second Affiliated Hospital of Xian Medical University
Priority date: 2021-03-09
Filing date: 2021-03-09
Publication date: 2021-04-30

Abstract

The invention discloses a medicine for treating osteoporosis and a preparation method thereof, wherein the medicine comprises the following components in parts by weight: 5-10 parts of rhizoma cibotii, 5-10 parts of teasel root, 5-10 parts of gentiana macrophylla, 5-10 parts of notopterygium root, 3-8 parts of peach kernel, 3-8 parts of safflower, 3-8 parts of angelica, 3-8 parts of ligusticum wallichii, 3-8 parts of myrrh, 3-10 parts of trogopterus dung, 3-10 parts of rhizoma cyperi, 3-10 parts of achyranthes bidentata, 4-6 parts of earthworm, 3-6 parts of liquorice, 5-10 parts of fructus cnidii and 5-10 parts of epimedium herb. The medicine can effectively solve the problem of poor treatment effect of the existing medicine formula.

Description

Medicine for treating osteoporosis and treatment method thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicines, in particular to a medicine for treating osteoporosis and a treatment method thereof.

Background

Osteoporosis is a systemic or systemic skeletal disease characterized by a decrease in bone mass, destruction of the microstructure of bone tissue, and an increase in bone fragility, which leads to an increase in the risk of fracture. With the aging world population, the disease has an increased incidence and is associated with serious complications, which place enormous physical and mental burdens on society and patients, and thus, the world health organization classifies it as one of four major elderly diseases.

Osteoporosis is caused by various factors, and the basic pathological mechanism of the osteoporosis is that the coupling of bone absorption and bone formation in the bone metabolic process is defective, so that the calcium and phosphorus metabolism in a human body is unbalanced, and the bone density is gradually reduced to cause clinical symptoms. The currently commonly used medicines for treating osteoporosis mainly comprise calcium agents, active vitamin D, estrogen replacement therapy, parathyroid hormone, diphosphonates and the like. These drugs suffer from the following disadvantages: the use of calcium agents alone has physiological absorption limits; active vitamin D is easy to cause hypercalcemia and hypercalcemia; the estrogen replacement therapy is a common means for preventing and treating postmenopausal osteoporosis, has strong effects of inhibiting bone resorption and promoting bone formation, and has side effects of inducing risks of breast cancer, endometrial cancer and the like; parathyroid hormone, although it promotes bone formation, has no established influence on endocrine system and clinical use; bisphosphonates are currently used in a wide range of applications, interfering with various biochemical functions of osteoclasts to inhibit bone resorption, but also interfering with the normal bone remodeling process of the body, which has many adverse effects over long-term use. Therefore, the development of the traditional Chinese medicine preparation for improving the osteoporosis and having small toxic and side effects is significant.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides a medicine for treating osteoporosis and a preparation method thereof, and the medicine can effectively solve the problem of poor treatment effect of the existing medicine formula.

In order to achieve the purpose, the technical scheme adopted by the invention for solving the technical problems is as follows:

the medicine for treating osteoporosis comprises the following components in parts by weight: 5-10 parts of rhizoma cibotii, 5-10 parts of teasel root, 5-10 parts of gentiana macrophylla, 5-10 parts of notopterygium root, 3-8 parts of peach kernel, 3-8 parts of safflower, 3-8 parts of angelica, 3-8 parts of ligusticum wallichii, 3-8 parts of myrrh, 3-10 parts of trogopterus dung, 3-10 parts of rhizoma cyperi, 3-10 parts of achyranthes bidentata, 4-6 parts of earthworm, 3-6 parts of liquorice, 5-10 parts of fructus cnidii and 5-10 parts of epimedium herb.

Further, the paint comprises the following components in parts by weight: 9 parts of rhizoma cibotii, 9 parts of teasel root, 6 parts of large-leaved gentian, 6 parts of notopterygium root, 6 parts of peach seed, 4 parts of safflower, 8 parts of angelica, 8 parts of ligusticum wallichii, 6 parts of myrrh, 6 parts of trogopterus dung, 8 parts of rhizoma cyperi, 10 parts of achyranthes bidentata, 6 parts of earthworm, 5 parts of liquorice, 10 parts of fructus cnidii and 10 parts of epimedium herb.

In the scheme, the epimedium herb is pungent, sweet and warm in nature and has the effect of moving the liver and kidney meridians; the traditional Chinese medicine is a key medicine for tonifying the Mingmen, benefiting vital essence and qi, strengthening bones and muscles, tonifying kidney and strengthening yang, and modern researches show that the traditional Chinese medicine has the effects of inhibiting bone resorption, promoting the generation of bone cells and increasing calcified bones.

Large-leaf gentian root, radix gentianae macrophyllae, pungent, bitter and mild in flavor, enters stomach, liver and gallbladder meridians, and has the effects of dispelling wind-damp, clearing away damp-heat and relieving arthralgia. Can be used for treating spasm of tendons and vessels, soreness of bone joints, and hectic fever.

Notopterygium root, rhizoma Et radix Notopterygii, being pungent, bitter and warm in flavor, enters bladder and kidney meridians, and has the effects of dispelling exterior cold, expelling wind-damp, benefiting joint and relieving pain.

Fructus Cnidii is warm in nature and bitter in taste, and has effects of eliminating dampness, dispelling pathogenic wind, warming kidney and tonifying yang. The traditional Chinese medicine composition is used for treating cold-dampness leukorrhea and damp arthralgia lumbago, and modern researches show that the traditional Chinese medicine composition has the effect of promoting osteoblast increase.

Peach kernel has the functions of promoting blood circulation to disperse blood clots, relaxing bowels, relieving cough and asthma. Can be used for treating traumatic injury, constipation due to intestinal dryness, cough and asthma.

Safflower is pungent and warm in nature, has the effects of promoting blood circulation, dredging channels, removing blood stasis and relieving pain, and is helpful for treating thoracic obstruction, heart pain, blood stasis, abdominal pain, stabbing pain in chest and hypochondrium, traumatic injury, sore and ulcer with swelling and pain.

The angelica has warm nature and sweet taste, and has the effects of activating blood, regulating menstruation, relieving pain, moistening dryness and lubricating intestines. Treating blood deficiency syndromes, deficiency cold abdominal pain, flaccidity arthralgia, numbness of skin and traumatic injury.

The rhizoma ligustici wallichii is pungent and warm in nature and has the effects of promoting qi circulation, relieving depression, dispelling wind dryness and dampness, promoting blood circulation and relieving pain. Can be used for treating traumatic injury, swelling and pain, headache, and rheumatic arthralgia.

The myrrh has the property of bitter and pungent, has the functions of promoting blood circulation, removing blood stasis, promoting qi circulation and relaxing muscles and tendons, and has the treatment effect on traumatic injury and muscle and bone pain.

Oletum Trogopterori has effects of promoting blood circulation and relieving pain.

The nutgrass galingale rhizome is pungent, warm, bitter and sweet in nature and has the functions of regulating qi, resolving depression, regulating menstruation and relieving pain. Can be used for treating stagnation of qi due to stagnation of liver-qi, chest, hypochondrium, abdominal distention and pain, dyspepsia, menoxenia, amenorrhea, dysmenorrhea, cold hernia, abdominal pain, and breast pain.

Achyranthes root is bitter and sweet in taste, has the effects of activating blood circulation to promote menstruation, tonifying liver and kidney and strengthening waist and knees, and is used for treating soreness and pain of waist and knees, liver and kidney deficiency and traumatic blood stasis and pain. The veterinary drug is used for treating cattle foot softening disease, bone fracture due to falling injury, and the like.

Lumbricus is cold in nature and salty in taste, and has effects of clearing heat, relieving spasm, relieving asthma, and promoting urination. Can be used for treating hyperpyrexia, mania, convulsion, headache, conjunctival congestion, asthma, phlegm heat, apoplexy, and hemiplegia.

The liquorice has the effects of tonifying spleen and qi, clearing away heat and toxic materials, eliminating phlegm and stopping cough, relieving spasm and pain and harmonizing the drugs, and modern researches show that the liquorice has an anti-inflammatory effect.

Xu Duan is warm in nature, bitter and pungent in flavor, and enters liver and kidney meridians. Has effects in nourishing liver and kidney, strengthening muscle and bone, promoting fracture, and relieving metrorrhagia and metrostaxis, and can be used for treating deficiency of liver and kidney, soreness of waist and knees, rheumatalgia, tendon injury, fracture, metrorrhagia, fetal leakage, and traumatic injury. Wherein, the wine teasel root is mainly used for treating rheumatism, arthralgia, tendon injury, fracture and traumatic injury, and the salt teasel root is mainly used for treating soreness and weakness of waist and knees.

Rhizoma Cibotii is bitter and sweet in taste, warm in nature, and enters liver and kidney meridians; hard mass moving and dissipating, descending and ascending; has effects of strengthening waist and knee, dispelling pathogenic wind and removing dampness, and invigorating kidney qi, and can be used for treating lumbago due to kidney deficiency, ankylosis, leg weakness, asthenia, and rheumatalgia.

The preparation method of the medicine for treating osteoporosis comprises the following steps:

(1) mixing the above materials, coarse pulverizing, adding water, soaking for 20-40min, reflux extracting for 1-3 hr, separating to obtain first extractive solution and residue, adding water to residue, adjusting pH to 3-5, reflux extracting for 1-2 hr, and separating to obtain second extractive solution and residue;

(2) continuously adding 60-80% ethanol solution into the filter residue, reflux-extracting for 1-2h, separating to obtain a third extractive solution and filter residue, continuously adding 60-80% ethanol into the filter residue, adjusting pH to 3-5, reflux-extracting for 1-2h, and separating to obtain a fourth extractive solution;

(3) mixing the first extractive solution, the second extractive solution, the third extractive solution and the fourth extractive solution, concentrating to obtain extract with relative specific density of 1.20-1.25, adding adjuvants, and making into corresponding preparation.

In the scheme, the medicine mixture is extracted in water and ethanol solution respectively, and due to the different polarities of the medicine mixture and the ethanol solution, the effective components with different polarities in the medicinal materials can be dissolved in the water and ethanol solution respectively according to the similar compatibility principle, so that the effective components in the medicinal materials are fully extracted, and the treatment effect of the medicine is improved.

In the extraction process by using water and ethanol as solvents, the pH value of the solution is adjusted to simulate the acidic environment in intestinal tracts in vitro, so that the solubility of active ingredients in medicinal materials can be further improved, and the treatment effect of the medicament is improved.

Further, in the step (1), the volume ratio of the first water adding amount to the medicine mixture is 2-4:1, and the volume ratio of the second water adding amount to the filter residue is 1-3: 1.

In the above scheme, the volume of the solvent is too large, so that the waste of the solvent is caused, the time for subsequent concentration and the energy consumption are prolonged, the volume of the solvent is too small, the dissolution rate of the active ingredients in the medicinal materials is poor, the active ingredients are reduced, and the treatment effect is reduced.

Further, in the step (1), the time for the first reflux extraction is 2 hours, and the time for the second reflux extraction is 1 hour.

In the scheme, the medicinal materials are always in a high-temperature environment, the extraction time is too long, the decomposition and the damage of the effective components can be caused, the treatment effect is reduced, the extraction time is too short, and the effective components cannot be fully dissolved out.

Further, in the step (1), the pH value of the solution is adjusted to 4.

Further, in the step (2), the volume ratio of the first ethanol addition to the filter residue is 1-3:1, and the volume ratio of the second ethanol addition to the filter residue is 1-2: 1.

Further, in the step (2), the time for the first reflux extraction is 1.5h, and the time for the second reflux extraction is 1 h.

Further, the pH value of the solution is adjusted to 4 in the step (2).

Further, the volume concentration of ethanol in the step (2) is 75%.

In the scheme, the polarities of the ethanol solutions with different volume concentrations are different, and when the ethanol with the volume concentration of 75% is selected as a solvent, effective ingredients can be fully dissolved out, so that the treatment effect of the medicine is improved.

Further, the preparation formulation is pills or granules.

The beneficial effects produced by the invention are as follows: the traditional Chinese medicine theory considers that the relationship between the skeletal development of human body and kidney is close, the kidney stores essence, essence produces marrow, the marrow is in the bone, and the growth and development of the skeleton depend on the abundance of the marrow and the nutrition provided by the marrow. According to the invention, the epimedium has the effects of benefiting vital essence and qi, tonifying kidney and supporting yang, the fructus cnidii has the effects of warming kidney and tonifying yang, the radix dipsaci has the effect of tonifying liver and kidney, and the rhizoma cibotii has the effect of reinforcing kidney qi; the gentiana macrophylla, the fructus cnidii and the notopterygium root are used for dispelling wind-cold-dampness, so that pain caused by cold-dampness can be reduced, damage of exogenous pathogenic factors to bones can be reduced, exogenous pathogenic factors are eliminated, and the growth and development speed of the bones is increased; peach kernel, safflower, angelica and rhizoma ligustici wallichii are used for activating blood and removing stasis, after stasis is eliminated, blood circulation is smooth, nutrition supply is sufficient, and bone growth can be accelerated; the myrrh, the trogopterus dung and the rhizoma cyperi are used for promoting qi and blood circulation and relieving pain, smooth qi movement, accelerated blood circulation and promoting the recovery of body functions; achyranthis radix and Lumbricus can be used for dredging channels and collaterals, promoting blood circulation, and accelerating nutrition supply. The formula of the invention jointly acts on the body of a patient through medicines of activating blood circulation and stimulating the menstrual flow, dispelling wind and removing dampness, tonifying kidney and producing sperm and the like, promotes the growth and development of bones, and has reasonable matching and synergistic effect among the medicines, so that the osteoporosis can be treated.

From the perspective of modern medicine, epimedium, cnidium fruit, teasel root and cibotium rhizome have the functions of inhibiting bone resorption, promoting bone cell generation and bone calcification, and enhancing bone density, so that osteoporosis can be relieved and treated.

Detailed Description

Example 1

The medicine for treating osteoporosis comprises the following components in parts by weight: 5 parts of rhizoma cibotii, 10 parts of teasel root, 5 parts of large-leaved gentian, 6 parts of notopterygium root, 4 parts of peach seed, 5 parts of safflower, 4 parts of angelica, 4 parts of ligusticum wallichii, 4 parts of myrrh, 5 parts of trogopterus dung, 4 parts of rhizoma cyperi, 4 parts of achyranthes root, 5 parts of earthworm, 3 parts of liquorice, 6 parts of fructus cnidii and 6 parts of epimedium herb.

(1) mixing the above medicines, coarsely crushing, adding water, soaking for 40min at a volume ratio of water to medicine mixture of 4:1, reflux-extracting for 1h, separating to obtain first extractive solution and residue, adding water to residue at a volume ratio of water to medicine mixture of 2:1, adjusting pH to 3, reflux-extracting for 1h, and separating to obtain second extractive solution and residue;

(2) continuously adding 60% ethanol solution into the filter residue, performing reflux extraction for 2h, wherein the volume ratio of the ethanol addition to the filter residue is 3:1, separating to obtain a third extract and the filter residue, continuously adding 60% ethanol into the filter residue, wherein the volume ratio of the ethanol addition to the filter residue is 2:1, adjusting the pH value of the solution to 4, performing reflux extraction for 1h, and separating to obtain a fourth extract;

(3) and mixing the first extract, the second extract, the third extract and the fourth extract, concentrating to obtain an extract with a relative ratio density of 1.20, adding auxiliary materials, and preparing into corresponding preparations.

Example 2

The medicine for treating osteoporosis comprises the following components in parts by weight: 9 parts of rhizoma cibotii, 8 parts of teasel root, 8 parts of large-leaved gentian, 8 parts of notopterygium root, 7 parts of peach seed, 6 parts of safflower, 6 parts of angelica, 8 parts of ligusticum wallichii, 8 parts of myrrh, 8 parts of trogopterus dung, 8 parts of rhizoma cyperi, 8 parts of achyranthes bidentata, 5 parts of earthworm, 6 parts of liquorice, 8 parts of fructus cnidii and 9 parts of epimedium herb.

(1) mixing the above medicines, coarsely crushing, adding water, soaking for 20min at a volume ratio of water to medicine mixture of 2:1, reflux-extracting for 3h, separating to obtain first extractive solution and residue, adding water to residue at a volume ratio of water to residue of 2:1, adjusting pH to 5, reflux-extracting for 1h, and separating to obtain second extractive solution and residue;

(2) continuously adding an ethanol solution with the volume concentration of 80% into the filter residue, performing reflux extraction for 2 hours, wherein the volume ratio of the ethanol addition to the filter residue is 3:1, separating to obtain a third extracting solution and the filter residue, continuously adding 80% ethanol into the filter residue, wherein the volume ratio of the ethanol addition to the filter residue is 2:1, adjusting the pH value of the solution to be 5, performing reflux extraction for 2 hours, and separating to obtain a fourth extracting solution;

(3) and mixing the first extracting solution, the second extracting solution, the third extracting solution and the fourth extracting solution, concentrating to obtain an extract with a relative ratio density of 1.25, adding auxiliary materials, and preparing into corresponding preparations.

Example 3

The medicine for treating osteoporosis comprises the following components in parts by weight: 9 parts of rhizoma cibotii, 9 parts of teasel root, 6 parts of large-leaved gentian, 6 parts of notopterygium root, 6 parts of peach seed, 4 parts of safflower, 8 parts of angelica, 8 parts of ligusticum wallichii, 6 parts of myrrh, 6 parts of trogopterus dung, 8 parts of rhizoma cyperi, 10 parts of achyranthes bidentata, 6 parts of earthworm, 5 parts of liquorice, 10 parts of fructus cnidii and 10 parts of epimedium herb.

(1) mixing the above medicines, coarsely crushing, adding water, soaking for 30min at a volume ratio of water to medicine mixture of 3:1, reflux-extracting for 2h, separating to obtain first extractive solution and residue, adding water to residue at a volume ratio of water to medicine mixture of 2:1, adjusting pH to 4, reflux-extracting for 1h, and separating to obtain second extractive solution and residue;

(2) continuously adding 75% ethanol solution into the filter residue, reflux-extracting for 1.5h, wherein the volume ratio of the ethanol addition to the filter residue is 2:1, separating to obtain a third extract and the filter residue, continuously adding 75% ethanol into the filter residue, wherein the volume ratio of the ethanol addition to the filter residue is 2:1, adjusting the pH value of the solution to 4, reflux-extracting for 1h, and separating to obtain a fourth extract;

Comparative example 1

The medicine for treating osteoporosis comprises the following components in parts by weight: 9 parts of teasel root, 6 parts of large-leaved gentian, 6 parts of notopterygium root, 6 parts of peach kernel, 8 parts of angelica, 8 parts of ligusticum wallichii, 6 parts of myrrh, 6 parts of trogopterus dung, 8 parts of rhizoma cyperi, 10 parts of achyranthes root, 6 parts of earthworm, 5 parts of liquorice and 10 parts of epimedium herb.

The preparation method of the medicine for treating osteoporosis is the same as that of example 3.

Comparative example 2

The medicine for treating osteoporosis comprises the following components in parts by weight: the traditional Chinese medicine composition comprises, by weight, 9 parts of cortex acanthopanacis, 9 parts of teasel root, 6 parts of large-leaved gentian, 6 parts of radix angelicae pubescentis, 6 parts of peach kernel, 4 parts of safflower, 8 parts of angelica sinensis, 8 parts of ligusticum wallichii, 6 parts of myrrh, 6 parts of trogopterus dung, 8 parts of rhizoma cyperi, 10 parts of radix achyranthis bidentatae, 6 parts of earthworm, 5 parts of liquorice, 10 parts of hippocampus and 10 parts of cistanche.

Comparative example 3

The medicine for treating osteoporosis comprises the following components in parts by weight: 3 parts of rhizoma cibotii, 3 parts of teasel root, 6 parts of large-leaved gentian, 3 parts of notopterygium root, 6 parts of peach seed, 4 parts of safflower, 8 parts of angelica, 12 parts of ligusticum wallichii, 6 parts of myrrh, 6 parts of trogopterus dung, 8 parts of rhizoma cyperi, 14 parts of achyranthes root, 8 parts of earthworm, 5 parts of liquorice, 3 parts of fructus cnidii and 6 parts of epimedium herb.

Comparative example 4

(1) mixing the above medicines, coarsely crushing, adding water, soaking for 30min at a volume ratio of water to medicine mixture of 3:1, reflux-extracting for 4h, separating to obtain first extractive solution and residue, adding water to residue at a volume ratio of water to medicine mixture of 2:1, reflux-extracting for 3h, and separating to obtain second extractive solution and residue;

(2) continuously adding 50% ethanol solution into the filter residue, performing reflux extraction for 3h, wherein the volume ratio of the ethanol addition to the filter residue is 2:1, separating to obtain a third extract and the filter residue, continuously adding 50% ethanol into the filter residue, wherein the volume ratio of the ethanol addition to the filter residue is 2:1, performing reflux extraction for 3h, and separating to obtain a fourth extract;

Test examples

Randomly finding 140 patients with osteoporosis at the age of 40-60 years, measuring and recording the bone density of each patient, then randomly dividing the patients into 7 groups of 20 patients, wherein the average age of each group is 48-49 years, each group has comparability, respectively taking the medicaments in the 7 groups of the patients according to the examples 1-3 and the comparative examples 1-4 three times a day and 20g each time for 2 months, measuring and recording the bone density of each patient, taking the average bone density of all the patients in each group as the final result of the group, and calculating and recording the effective rate of each group of the patients, wherein the specific result is shown in the table 1-2.

Treatment criteria were:

the effect is shown: the patient has increased bone density but does not reach the normal range;

the method has the following advantages: the bone density of the patient is recovered to a normal value;

and (4) invalidation: the bone density of the patients has no obvious change.

Effective rate is 100% of effective people/total people

Table 1: patient treatment for bone density conditions before and after treatment

As can be seen from the results in the above table, the drugs in examples 1-3 have a certain therapeutic effect on osteoporosis, and can significantly improve the bone density of patients, while the drugs in comparative examples 1-4 also have a certain therapeutic effect, but have poor therapeutic effects.

Table 2: statistics of effective rate

	Total number of people (human)	Number of effective people (human)	Number of valid persons (human)	Invalid number of people (human)	Effective rate (%)
						Example 1	20	20	18	0	90％
Example 2	20	20	18	0	90％
						Example 3	20	20	20	0	100％
Comparative example 1	20	13	2	7	10％
						Comparative example 2	20	14	3	6	15％
Comparative example 3	20	14	2	6	10％
						Comparative example 4	20	15	4	5	20％

As can be seen from the data in the table above, the effective rates in examples 1-3 are all higher than those in comparative examples 1-4, which proves that the drugs in examples 1-3 have better therapeutic effect on osteoporosis.

Comparing comparative example 1 with example 3, it can be seen that the treatment effect of the formula is reduced after the addition of rhizoma cibotii, fructus cnidii and safflower in the formula is cancelled, which proves that the formula of the application is an integral body, the medicinal materials in the formula have interaction, and the integral effect of the formula is reduced after one or more of the medicinal materials are lacked.

Comparing the comparative example 2 with the example 3, the rhizoma cibotii is replaced by the cortex acanthopanacis with similar effect, the notopterygium root is replaced by the radix angelicae pubescentis, the fructus cnidii is replaced by the hippocampus, and the herba epimedii is replaced by the herba cistanches, although the replaced medicine has similar effect with the medicine before replacement, the whole effect of the formula is still poor, and the interaction between different medicinal materials in the application is proved.

Comparing the comparative example 3 with the example 3, the proportion relation in the formula is changed, although the components in the formula are not changed, the whole effect of the formula is reduced after the dosage is changed, and the application proves that the formula is a whole and any factor is changed to influence the treatment effect.

Comparing comparative example 4 with example 3, in comparative example 4, the extraction method of the medicinal materials is changed, resulting in the decrease of the therapeutic effect of the drug, and it is also confirmed that the preparation method in the present application is different from the conventional preparation method, which can increase the extraction amount of the effective ingredients.

Claims

1. The medicine for treating osteoporosis is characterized by comprising the following components in parts by weight: 5-10 parts of rhizoma cibotii, 5-10 parts of teasel root, 5-10 parts of gentiana macrophylla, 5-10 parts of notopterygium root, 3-8 parts of peach kernel, 3-8 parts of safflower, 3-8 parts of angelica, 3-8 parts of ligusticum wallichii, 3-8 parts of myrrh, 3-10 parts of trogopterus dung, 3-10 parts of rhizoma cyperi, 3-10 parts of achyranthes bidentata, 4-6 parts of earthworm, 3-6 parts of liquorice, 5-10 parts of fructus cnidii and 5-10 parts of epimedium herb.

2. The medicament for treating osteoporosis as claimed in claim 1, which comprises the following components in parts by weight: 9 parts of rhizoma cibotii, 9 parts of teasel root, 6 parts of large-leaved gentian, 6 parts of notopterygium root, 6 parts of peach seed, 4 parts of safflower, 8 parts of angelica, 8 parts of ligusticum wallichii, 6 parts of myrrh, 6 parts of trogopterus dung, 8 parts of rhizoma cyperi, 10 parts of achyranthes bidentata, 6 parts of earthworm, 5 parts of liquorice, 10 parts of fructus cnidii and 10 parts of epimedium herb.

3. The process for preparing a medicament for the treatment of osteoporosis of claim 1 or 2, comprising the steps of:

(1) mixing the above materials, adding water, soaking for 20-40min, reflux extracting for 1-3 hr, separating to obtain first extractive solution and residue, adding water to the residue, adjusting pH to 3-5, reflux extracting for 1-2 hr, and separating to obtain second extractive solution and residue;

4. The method for preparing a medicament for treating osteoporosis as claimed in claim 3, wherein the volume ratio of the first water addition amount to the medicament mixture in step (1) is 2-4:1, and the volume ratio of the second water addition amount to the residue is 1-3: 1.

5. The method for preparing a medicament for treating osteoporosis as claimed in claim 3, wherein the first reflux extraction time in step (1) is 2 hours and the second reflux extraction time is 1 hour.

6. The method for preparing a medicament for treating osteoporosis as claimed in claim 3, wherein the volume ratio of the first ethanol addition amount to the residue in step (2) is 1-3:1, and the volume ratio of the second ethanol addition amount to the residue is 1-2: 1.

7. The method for preparing a medicament for treating osteoporosis as claimed in claim 3, wherein the first reflux extraction time in step (2) is 1.5 hours and the second reflux extraction time is 1 hour.

8. The method for preparing a medicament for treating osteoporosis as claimed in claim 3, wherein the ethanol concentration in step (2) is 75% by volume.

9. The method for preparing a medicament for treating osteoporosis of claim 3, wherein the formulation is in the form of pill or granule.