CN114988949A - Preparation method and application of enzyme fertilizer for improving yield and quality of fruits and vegetables - Google Patents
Preparation method and application of enzyme fertilizer for improving yield and quality of fruits and vegetables Download PDFInfo
- Publication number
- CN114988949A CN114988949A CN202210704546.XA CN202210704546A CN114988949A CN 114988949 A CN114988949 A CN 114988949A CN 202210704546 A CN202210704546 A CN 202210704546A CN 114988949 A CN114988949 A CN 114988949A
- Authority
- CN
- China
- Prior art keywords
- parts
- fertilizer
- fruits
- quality
- yield
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Inorganic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to the technical field of compound biofertilizers, in particular to a preparation method and application of a ferment fertilizer for improving the yield and quality of fruits and vegetables, wherein the ferment fertilizer comprises the following ingredients: 20-30 parts of composite straw powder such as edible fungus residues, waxy corn green stalks, tomatoes, watermelons, melon green stalks and the like; 10-15 parts of brown sugar; 1-2 parts of bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens and 2-4 parts of EM composite microbial inoculum; 6-8 parts of a fruit and vegetable stock solution; 5-7 parts of vinasse; 5-6 parts of rape oil meal; 3-5 parts of traditional Chinese medicine residues; 5-6 parts of cow dung; 4-6 parts of biomass charcoal; 3-5 parts of a multi-chain ethylene high polymer; 2-3 parts of wheat bran; 1-2 parts of alginic acid; a fertilizer synergist. The ferment fertilizer of the invention is prepared only in 30-37 days, and has short fermentation time and lower time cost.
Description
Technical Field
The invention relates to the technical field of compound biofertilizers, in particular to a preparation method and application of a ferment fertilizer for improving the yield and quality of fruits and vegetables.
Background
The unreasonable use of industrial fertilizers not only causes huge resource waste, but also causes deterioration of natural ecology and environmental pollution. With the national demand for ecological agriculture and sustainable agriculture, and the rise of global green food production and consumption, the development of green and nontoxic biological fertilizers becomes a hot tide. Biological fertilizers refer to a class of biological products rich in effective active microorganisms, which exert their specific fertilizer effects by improving the growth environment, nutritional conditions, conversion of nutrients of crops through the activity of microorganisms and inhibiting the biological activity of harmful microorganisms. At present, microbial fertilizers are divided into two categories, one is that the supply of plant nutrient elements is increased through the life activity of microbes contained in the fertilizer, and the nutrient condition of plants is improved; the other is that the plant growth hormone produced by the contained microorganism is utilized to promote the absorption and utilization of nutrient elements by plants, or the pathogenic action of antagonistic pathogenic microorganisms is utilized to relieve the plant diseases and insect pests of crops so as to increase the yield of the crops. The biological fertilizer is mainly applied to rhizobia fertilizers, azotobacter fertilizers, phosphorus bacterial fertilizers, silicate fertilizers, VA mycorrhizal fungi fertilizers and compound microbial fertilizers on a large scale, and is also originated from 5406 bacterial fertilizers, azospirillum, Plant Growth Promoting Rhizobacteria (PGPR) and the like in China. In recent years, the microbial enzyme fertilizer contains active microorganisms, microbial metabolites and various minerals, can reduce phosphorus, potassium and trace elements absorbed and dissolved by fertilizer and pesticide, and can increase the content of available nutrients in soil, so the microbial enzyme fertilizer is selected as an ideal green fertilizer.
China is one of the main countries for producing fruits in the world, the total yield of fruits reaches 2400 ten thousand tons every year, and the number of the fruits is second to the yields of grains, oil materials and sugar materials, and is the 4 th of the yield of main agricultural products in China. However, in China, the fruit residues are not reasonably utilized, and even some of the fruit residues are discarded as garbage, so that the environment is polluted. The ferment fertilizer is produced by fermenting the fruit residues, so that the environmental pollution and the resource waste are reduced, the use amount of the pesticide of the cash crops is reduced, and the green planting, the ecological planting and the healthy planting are accelerated.
In the prior art, the patent application for 'plant enzyme biological fertilizer and a preparation method thereof' (application number: 201410807328.4) provides a plant enzyme biological fertilizer which is prepared by taking brown sugar, fresh plants or fruits and vegetables and water as raw materials, sealing, storing, fermenting for 80-100 days, and filtering.
The patent application 'fermented ferment fertilizer and a preparation and use method thereof' (application number: 201510162424.2) is that vegetable garbage in facility agriculture and/or vegetable garbage in agricultural product trade markets are cut into pieces, vegetables, brown sugar and water are mixed according to a certain mass ratio, and the mixture is subjected to sealed fermentation for more than 3 months to obtain the fermented ferment fertilizer.
In the prior art, as no special bacterial liquid for fermentation is added in the fermentation process, the time for fermenting the materials is long, and the production performance of the produced ferment fertilizer needs to be further improved.
Disclosure of Invention
In order to overcome the defects, the invention provides a preparation method and application of an enzyme fertilizer for improving the yield and quality of fruits and vegetables.
The technical implementation scheme of the invention is as follows:
a preparation method of ferment fertilizer for improving the yield and quality of fruits and vegetables comprises the following ingredients: 20-30 parts of composite straw powder such as edible fungus residues, waxy corn green stalks, tomatoes, watermelons, melon green stalks and the like; 10-15 parts of brown sugar; 1-2 parts of bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens and 2-4 parts of EM composite microbial inoculum; 6-8 parts of a fruit and vegetable stock solution; 5-7 parts of vinasse; 5-6 parts of rape oil meal; 3-5 parts of traditional Chinese medicine residues; 5-6 parts of cow dung; 4-6 parts of biomass charcoal; 3-5 parts of a multi-chain ethylene high polymer; 2-3 parts of wheat bran; 1-2 parts of alginic acid; a fertilizer synergist.
Preferably, the preparation method of the ferment fertilizer for improving the yield and the quality of the fruits and the vegetables comprises the following steps:
(1) taking waxy corn green stalks, edible fungus residues, tomatoes, watermelons and melon green stalks according to the mass ratio of 1:3:2:2:1, crushing the waxy corn green stalks, the edible fungus residues, the tomatoes, the watermelons and the melon green stalks into straw powder, and performing ultraviolet sterilization treatment for 30 min;
(2) treating cow dung with ozone for 10-20 min. Adding water and brown sugar into straw powder and wheat bran, mixing well, adding bacillus subtilis and bacillus amyloliquefaciens, sealing, and standing at 25-28 deg.C for 25-30 days to obtain A;
(3) mixing fruit and vegetable stock solution, distiller's grains, rape oil meal and Chinese medicinal residue with water, adding Bacillus licheniformis, sealing, and standing at 25-28 deg.C for 5-7 days to obtain B;
(4) adding biomass charcoal, multi-chain ethylene high polymer, alginic acid and a fertilizer synergist into the mixture A and B to form a mixture C;
(5) and drying, sieving, subpackaging and ultraviolet sterilizing the composition C to obtain the ferment fertilizer for improving the yield and the quality of the fruits and vegetables.
Preferably, the application method of the ferment fertilizer for improving the yield and the quality of the fruits and vegetables comprises the following steps: it is used as base fertilizer for fruit and vegetable and is matched with compound fertilizer.
Preferably, in the application method of the ferment fertilizer for improving the yield and quality of fruits and vegetables, 400 jin of the organic fertilizer is used per mu; 30-40kg of compound fertilizer per mu, and applying the compound fertilizer in the sowing and fertilizing integrated machine.
The invention has the following advantages:
(1) the ferment fertilizer contains a large number of growth promoting factors, can increase the disease resistance of crops, greatly reduces pesticide residues, and promotes the growth and development of the crops.
(2) The ferment fertilizer also contains a large amount of amino acid and nucleic acid, and can be directly absorbed by plants, thereby improving the metabolic capability of the plants and the growth speed of crops.
(3) The ferment fertilizer of the invention is prepared only in 30-37 days, and has short fermentation time and lower time cost.
(4) The ferment fertilizer disclosed by the invention is simple in preparation process, easily available in raw materials and low in production cost, can reduce the pollution of the pomace waste to the environment, and is convenient for realizing industrial production.
Detailed Description
Reference herein to an embodiment means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the invention. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.
Examples
A preparation method of ferment fertilizer for improving the yield and quality of fruits and vegetables comprises the following ingredients:
20-30 parts of composite straw powder such as edible fungus residues, waxy corn green stalks, tomatoes, watermelons, melon green stalks and the like; 10-15 parts of brown sugar; 1-2 parts of bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens and 2-4 parts of EM composite microbial inoculum; 6-8 parts of a fruit and vegetable stock solution; 5-7 parts of vinasse; 5-6 parts of rape oil meal; 3-5 parts of traditional Chinese medicine residues; 5-6 parts of cow dung; 4-6 parts of biomass charcoal; 3-5 parts of a multi-chain ethylene high polymer; 2-3 parts of wheat bran; 1-2 parts of alginic acid; a fertilizer synergist.
Preferably, the preparation method of the ferment fertilizer for improving the yield and the quality of the fruits and the vegetables comprises the following steps: (1) taking waxy corn green stalks, edible fungus dregs, tomatoes, watermelons and melon green stalks according to the mass ratio of 1:3:2:2:1, crushing the waxy corn green stalks, the edible fungus dregs, the tomatoes, the watermelons and the melon green stalks into straw powder, and sterilizing the straw powder for 30min by using ultraviolet rays;
(2) treating cow dung with ozone for 10-20 min. Adding water and brown sugar into straw powder and wheat bran, mixing well, adding bacillus subtilis and bacillus amyloliquefaciens, sealing, and standing at 25-28 deg.C for 25-30 days to obtain A;
(3) mixing fruit and vegetable stock solution, distiller's grains, rape oil meal and Chinese medicinal residue with water, adding Bacillus licheniformis, sealing, and standing at 25-28 deg.C for 5-7 days to obtain B;
(4) adding biomass charcoal, multi-chain ethylene high polymer, alginic acid and a fertilizer synergist into the mixture A and B to form a mixture C;
(5) and drying, sieving, subpackaging and ultraviolet sterilizing the composition C to obtain the ferment fertilizer for improving the yield and the quality of the fruits and vegetables.
Preferably, the application method of the ferment fertilizer for improving the yield and the quality of the fruits and vegetables comprises the following steps: it is used as base fertilizer for fruit and vegetable and is matched with compound fertilizer.
Preferably, in the application method of the ferment fertilizer for improving the yield and quality of fruits and vegetables, 400 jin of the organic fertilizer is used per mu; 30-40kg of compound fertilizer per mu, and applying the compound fertilizer in the sowing and fertilizing integrated machine.
The invention has the following advantages:
(1) the ferment fertilizer contains a large number of growth promoting factors, can increase the disease resistance of crops, greatly reduces pesticide residues, and promotes the growth and development of the crops.
(2) The ferment fertilizer also contains a large amount of amino acid and nucleic acid, and can be directly absorbed by plants, so that the metabolic capability of the plants is improved, and the growth speed of crops is improved.
(3) The ferment fertilizer only needs 30-37 days for preparation, and has short fermentation time and lower time cost.
(4) The ferment fertilizer disclosed by the invention is simple in preparation process, easily available in raw materials and low in production cost, can reduce the pollution of the pomace waste to the environment, and is convenient for realizing industrial production.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (4)
1. The preparation method of the ferment fertilizer for improving the yield and the quality of fruits and vegetables is characterized by comprising the following ingredients: 20-30 parts of composite straw powder such as edible fungus residues, waxy corn green stalks, tomatoes, watermelons, melon green stalks and the like; 10-15 parts of brown sugar; 1-2 parts of bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens and 2-4 parts of EM composite microbial inoculum; 6-8 parts of a fruit and vegetable stock solution; 5-7 parts of vinasse; 5-6 parts of rape oil meal; 3-5 parts of traditional Chinese medicine residues; 5-6 parts of cow dung; 4-6 parts of biomass charcoal; 3-5 parts of a multi-chain ethylene high polymer; 2-3 parts of wheat bran; 1-2 parts of alginic acid; a fertilizer synergist.
2. The manufacturing method and application of the ferment fertilizer for improving the yield and quality of fruits and vegetables according to claim 1 are characterized in that the manufacturing method of the ferment fertilizer for improving the yield and quality of fruits and vegetables comprises the following steps:
(1) taking waxy corn green stalks, edible fungus dregs, tomatoes, watermelons and melon green stalks according to the mass ratio of 1:3:2:2:1, crushing the waxy corn green stalks, the edible fungus dregs, the tomatoes, the watermelons and the melon green stalks into straw powder, and sterilizing the straw powder for 30min by using ultraviolet rays;
(2) treating cow dung with ozone for 10-20min, adding water and brown sugar into straw powder and wheat bran, mixing, adding Bacillus subtilis and Bacillus amyloliquefaciens, sealing, and standing at 25-28 deg.C for 25-30 days to obtain A;
(3) mixing fruit and vegetable stock solution, distiller's grains, rape oil meal and Chinese medicinal residue with water, adding Bacillus licheniformis, sealing, and standing at 25-28 deg.C for 5-7 days to obtain B;
(4) adding biomass charcoal, multi-chain ethylene high polymer, alginic acid and a fertilizer synergist into the mixture A and B to form a mixture C;
(5) and drying, sieving, subpackaging and ultraviolet sterilizing the composition C to obtain the ferment fertilizer for improving the yield and the quality of the fruits and vegetables.
3. The manufacturing method and application of the ferment fertilizer for improving the yield and quality of fruits and vegetables according to claim 1 are characterized in that the application method of the ferment fertilizer for improving the yield and quality of fruits and vegetables comprises the following steps: it is used as base fertilizer for fruit and vegetable and is matched with compound fertilizer.
4. The method for manufacturing the ferment fertilizer for improving the yield and the quality of the fruits and the vegetables and the application thereof according to claim 1, wherein the method for applying the ferment fertilizer for improving the yield and the quality of the fruits and the vegetables comprises the steps of adding 400 jin of organic fertilizer per mu; 30-40kg of compound fertilizer per mu, and applying the compound fertilizer in the sowing and fertilizing integrated machine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210704546.XA CN114988949A (en) | 2022-06-21 | 2022-06-21 | Preparation method and application of enzyme fertilizer for improving yield and quality of fruits and vegetables |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210704546.XA CN114988949A (en) | 2022-06-21 | 2022-06-21 | Preparation method and application of enzyme fertilizer for improving yield and quality of fruits and vegetables |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114988949A true CN114988949A (en) | 2022-09-02 |
Family
ID=83037157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210704546.XA Pending CN114988949A (en) | 2022-06-21 | 2022-06-21 | Preparation method and application of enzyme fertilizer for improving yield and quality of fruits and vegetables |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114988949A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115772052A (en) * | 2022-12-23 | 2023-03-10 | 北京仁博堂中医研究院 | Animal manure organic fertilizer and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096319A (en) * | 2006-06-27 | 2008-01-02 | 荆向河 | Method for producing vegetable organic fertilizer by using edible fungus dregs |
| CN113698245A (en) * | 2021-09-09 | 2021-11-26 | 河南农业大学 | Biological organic fertilizer for improving sand ginger soil and preparation method and application thereof |
-
2022
- 2022-06-21 CN CN202210704546.XA patent/CN114988949A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096319A (en) * | 2006-06-27 | 2008-01-02 | 荆向河 | Method for producing vegetable organic fertilizer by using edible fungus dregs |
| CN113698245A (en) * | 2021-09-09 | 2021-11-26 | 河南农业大学 | Biological organic fertilizer for improving sand ginger soil and preparation method and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115772052A (en) * | 2022-12-23 | 2023-03-10 | 北京仁博堂中医研究院 | Animal manure organic fertilizer and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100572337C (en) | A kind of whole-fertilizer compound fertilizer and preparation method thereof | |
| CN102531720B (en) | Fermented biofertilizer prepared from waste vegetables, straw and livestock and poultry feces and preparation method thereof | |
| CN100528813C (en) | A biologic organic fertilizer, and preparation method | |
| AU2008331378B2 (en) | Microorganisms, microbial phosphate fertilizers and methods for preparing such microbial phosphate fertilizers | |
| CN101863695B (en) | Microbial organic fertilizer and preparation method thereof | |
| CN101289338B (en) | Biological salt-decomposition bacterial manure and method for preparing same | |
| CN106748341A (en) | Potato microbial bacterial manure special and preparation method thereof | |
| CN101712566B (en) | Preparation method of soil moisturizing bio-fertilizer | |
| CN104193431B (en) | A kind of feces of livestock and poultry harmless treatment specific complex microbial bacterial agent and preparation method | |
| CN101463334B (en) | Fermentation liquor composition for preparing biological organic fertilizer and preparation and use thereof | |
| CN106386163A (en) | Method for cultivating edible fungi by using crop straw | |
| CN101165008B (en) | Vegetable residual strain microorganism-earthworm multiple step inoculation conversion method | |
| CN103194405B (en) | Growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and microorganism organic fertilizer produced from growth-promoting bacteria | |
| CN102212494A (en) | Organic matter decomposing inoculant, and preparation method and application thereof | |
| CN104016748A (en) | Organic compound fertilizer and manufacturing method thereof | |
| CN104030751A (en) | Bioorganic compound fertilizer and preparation method thereof | |
| CN104774045B (en) | A kind of method that microbial organic bacterial manure is processed using sugar grass distiller's wort | |
| CN109180236A (en) | A kind of new process of aerobic fermentation processing chicken manure | |
| CN1974490A (en) | Process of fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure | |
| CN102910970A (en) | Method for preparing multifunctional Chinese medicinal organic bacterial manure | |
| CN102718596A (en) | Biological straw fertilizer, and production method and application thereof | |
| CN111646852A (en) | Efficient and environment-friendly composite biological organic fertilizer and preparation method thereof | |
| CN105198512B (en) | A kind of method that double-microbial fermentation prepares gamma-polyglutamic acid organic fertilizer | |
| CN103351188B (en) | Preparation method of agricultural organic fertilizer by treating pure straws as raw material | |
| CN110981635A (en) | Agricultural microbial agent with nitrogen fixation, phosphorus dissolution, potassium dissolution and cellulose dissolution functions and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |