CN114984196B

CN114984196B - Natural plant composition for preventing and treating arteriosclerosis, preparation method and application

Info

Publication number: CN114984196B
Application number: CN202210757395.4A
Authority: CN
Inventors: 孙美香; 潘建昂; 周术涛
Original assignee: Beijing Joy Time Biotechnology Co ltd
Current assignee: Beijing Joy Time Biotechnology Co ltd
Priority date: 2022-06-30
Filing date: 2022-06-30
Publication date: 2023-01-06
Anticipated expiration: 2042-06-30
Also published as: CN114984196A

Abstract

The invention provides a natural plant composition for preventing and treating arteriosclerosis, a preparation method and application thereof, wherein the natural plant composition comprises the following components in parts by mass: 100-200 parts of phytosterol, 100-200 parts of fish oil microcapsule powder, 10-40 parts of milk-benefiting polypeptide, 10-30 parts of red yeast rice, 30-70 parts of nattokinase, 1-5 parts of onion extract, 5-15 parts of mangosteen extract, 2-8 parts of broccoli seed extract and 5-25 parts of pomegranate extract. The natural plant composition can treat arteriosclerosis from the aspect of the formation mechanism of arteriosclerosis, thereby achieving the purpose of radical treatment.

Description

Natural plant composition for preventing and treating arteriosclerosis, preparation method and application

Technical Field

The invention relates to the technical field of treatment of arteriosclerosis, and particularly relates to a natural plant composition for preventing and treating arteriosclerosis, a preparation method and application thereof.

Background

Arteriosclerosis is a non-inflammatory disease of an artery, is a general name of degenerative and proliferative diseases of thickening, hardening and losing elasticity of an artery wall and narrowing a lumen, and is commonly known as atherosclerosis, medial calcification of an artery and arteriosclerotic 3. Arteriosclerosis is a vascular disease which appears with age, and the rule of the arteriosclerosis is usually that the arteriosclerosis occurs in adolescents, wherein the arteriosclerosis aggravates and develops in the elderly. The most important causes of arteriosclerosis are hypertension, hyperlipidemia and smoking. Other conditions such as obesity, diabetes, lack of exercise, stress, advanced age, family history, and irritable bowel syndrome can cause arteriosclerosis. In recent years, the disease is gradually increased in China and becomes one of the main causes of death of the old.

The mechanism of arteriosclerosis formation is that various pathogenic factors cause intimal damage to generate inflammation, mononuclear cells enter the intimal membrane, low-density lipoprotein cholesterol is oxidized, platelets are adhered and aggregated, smooth muscle cells proliferate and migrate to form a fibrous cap, vascular connective tissues are damaged, elastin cannot be well used, and then arteriosclerosis formation is caused.

The existing method for treating arteriosclerosis mainly treats arteriosclerosis symptomatically by using medicines, but the existing medicine treatment mode only prevents and treats arteriosclerosis by reducing indexes such as blood pressure, blood fat, blood sugar and the like, can relieve the disease condition, but often causes adverse reactions such as diarrhea, nausea, dizziness and the like, and cannot prevent and treat the arteriosclerosis fundamentally and needs to be taken for a lifetime.

In view of the above, the present invention is particularly proposed.

Disclosure of Invention

The invention aims to provide a natural plant composition for preventing and treating arteriosclerosis, which can effectively reduce the content of total cholesterol and low-density lipoprotein cholesterol and prevent platelet aggregation through mutual compatibility of a plurality of components, and can play roles in resisting inflammation and oxidation, repairing vascular intima and increasing vascular elasticity, thereby effectively preventing and treating arteriosclerosis.

The second purpose of the invention is to provide a preparation method of the natural plant composition, the preparation method is simple and convenient to operate, and the prepared composition has good prevention and treatment effects on arteriosclerosis.

The third purpose of the invention is to provide the application of the natural plant composition in medicines or health-care foods for treating diseases related to arteriosclerosis.

In order to achieve the above purpose of the present invention, the following technical solutions are adopted:

the invention provides a natural plant composition for preventing and treating arteriosclerosis, which comprises the following components in parts by mass: 100-200 parts of phytosterol, 100-200 parts of fish oil microcapsule powder, 10-40 parts of milk-benefiting polypeptide, 10-30 parts of red yeast rice, 30-70 parts of nattokinase, 1-5 parts of onion extract, 5-15 parts of mangosteen extract, 2-8 parts of broccoli seed extract and 5-25 parts of pomegranate extract.

Preferably, the food comprises 120-170 parts of phytosterol, 120-170 parts of fish oil microcapsule powder, 20-30 parts of milk polypeptide, 15-25 parts of red yeast rice, 40-60 parts of nattokinase, 1.5-4 parts of onion extract, 7-12 parts of mangosteen extract, 3-7 parts of broccoli seed extract and 10-20 parts of pomegranate extract.

Preferably 150 parts of phytosterol, 150 parts of fish oil microcapsule powder, 25 parts of milk benefiting polypeptide, 20 parts of red yeast rice, 50 parts of nattokinase, 2 parts of onion extract, 10 parts of mangosteen extract, 5 parts of broccoli seed extract and 15 parts of pomegranate extract.

In the prior art, arteriosclerosis is treated symptomatically mainly by using medicines, particularly, the arteriosclerosis is prevented and treated by reducing indexes such as blood pressure, blood fat, blood sugar and the like, although the mode can relieve the disease condition, adverse reactions such as diarrhea, nausea, dizziness and the like are often caused, the mode cannot be fundamentally prevented and treated, the treatment is not thorough, and the medicines are required to be taken for the whole life to control the disease condition.

In order to solve the above technical problems, the present invention provides a natural plant composition for preventing and treating arteriosclerosis, which prevents and treats arteriosclerosis from the viewpoint of arteriosclerosis formation mechanism, and achieves radical treatment of arteriosclerosis by:

1. reducing total cholesterol and low density lipoprotein cholesterol levels;

2. removing heavy metal toxin and endotoxin;

3. preventing platelet aggregation;

4. anti-inflammatory;

5. oxidation resistance;

6. repair the blood vessel intima and increase the blood vessel elasticity.

Specifically, when the components are compatible, the plant sterol and the red yeast rice are mutually cooperated as main materials, wherein the plant sterol mainly aims at exogenous cholesterol, can reduce the secretion of apolipoprotein B of intestinal tracts and liver cells, reduce the absorption of dietary cholesterol and the entrance of bile cholesterol into microbubbles, further reduce the absorption of the cholesterol, increase the secretion of bile acid, further improve the reverse transport of the cholesterol, and also can inhibit key enzyme (hydroxymethyl glutaryl coenzyme A reductase HMG-COA-R) for synthesizing the cholesterol to block the pathway for synthesizing the cholesterol. Monascus fungi in red yeast are inoculated to rice and prepared by fermentation, and contain a key enzyme (hydroxymethyl glutaryl coenzyme A reductase HMG-COA-R) specific inhibitor for human body to synthesize cholesterol, so that the endogenous pathway for synthesizing cholesterol can be blocked, and the monascus fungi not only can reduce total cholesterol, low density lipoprotein cholesterol and arteriosclerosis index, but also can reduce triglyceride and improve the remarkable comprehensive effect of high density lipoprotein cholesterol. The plant sterol and red rice can cooperate to play a synergistic role and inhibit endogenous cholesterol and exogenous cholesterol at the same time.

In the components of the invention, the broccoli seed extract is adopted to remove heavy metal toxin and endotoxin. Specifically, sulforaphane in broccoli seed extract can up-regulate transcription factor Nrf2, and can activate transcription of more than 500 genes in human genome, and most of the genes have cytoprotective function. Nrf2 produces cytoprotective effects through detoxification mechanisms that enhance detoxification and excretion of harmful foreign materials and toxic metals; nrf2 increases highly coordinated antioxidant activity through the action of more than 20 genes, which also has anti-inflammatory effects; in addition, nrf2 also enhances autophagy of cells to clear toxic protein aggregates and dysfunctional organelles, and the healthy nutrients (phenolic antioxidants, long-chain omega 3 fatty acids DHA and EPA, sulfides and terpenoids in allium sativum vegetables) are all acted upon by Nrf2 activity.

In the components of the invention, the nattokinase and the onion extract have the synergistic and cooperative effect. Specifically, nattokinase not only dissolves thrombus, but also inhibits platelet coagulation. The onion extract contains multiple active ingredients, wherein the polyphenol quercetin and sulfide isoalliin can inhibit platelet coagulation, and also has effects of inhibiting cholesterol oxidation, softening blood vessel, and inhibiting blood pressure increase. The two are matched with each other, so that good thrombolysis and platelet aggregation prevention effects can be achieved.

In the components of the invention, the fish oil microcapsule powder and the broccoli seed extract are compatible to play an anti-inflammatory role. In particular, the fish oil microcapsule powder contains omega 3 (EPA and DHA) fatty acids, which are capable of alleviating chronic inflammatory reactions. In fact, during the development of arteriosclerosis, the cell membrane and nucleus of immune cells are involved in inflammatory reactions, and when the vascular intima is damaged, monocytes in immune cells are activated. There are two molecules of inflammation, one is that inflammatory mediators acting on the cell membrane directly produce an inflammatory response including Prostaglandins (PG), leukotrienes (LT), and Thromboxanes (TX); the other is cell factor acting on cell nucleus, including tumor necrosis factor (TNF-alpha), interleukin IL-6, etc. Omega 3 polyunsaturated fatty acids can act not only on the nucleus but also on the cell membrane. Specifically, the omega 3 polyunsaturated fatty acid can inhibit NF-kB through PPARY, and can inhibit the nuclear expression of excessive proinflammatory cytokines, such as tumor necrosis factor TNF-alpha, interleukin IL-6 and the like. Meanwhile, omega 3 polyunsaturated fatty acids (EPA and DHA) competitively inhibit proinflammatory mediators derived from omega 6 polyunsaturated fatty acids (AA) on cell membranes, and the production of the proinflammatory mediators can be reduced. Omega 3 polyunsaturated fatty acids are furthermore capable of producing anti-inflammatory mediators with a direct resolution validation. The broccoli seed extract can play an anti-inflammatory role by up-regulating Nrf2 and reducing the activity of NF-kB and a series of inflammatory mediators (cytokines, chemokines, adhesion factors, COM-2, MMP-9, iNOS and the like). The two components cooperate with each other to achieve good anti-inflammatory effect.

The components of the invention also contain pomegranate extract and mangosteen extract. The two can be mutually compatible with broccoli seed extract to improve the multi-target synergistic antioxidation. Specifically, pomegranate extracts contain unique mixed polyphenols (punicalagin, anthocyanins, resveratrol and ellagic acid). Wherein, the punicalagin not only can activate the antioxidase, but also can inhibit the activity of lipid peroxidation; the anthocyanin is a strong antioxidant and has the functions of reducing the permeability and weakness of capillary vessels; resveratrol is a strong antioxidant and has the effects of inhibiting nucleotide reductase, and inhibiting low density lipoprotein cholesterol oxidation and lipid peroxidation; ellagic acid also has antioxidant and anti-inflammatory effects. The mangosteen extract contains various polyphenols such as xanthones, procyanidins, catechins, etc., which are good antioxidants. Nrf2 increases highly coordinated antioxidant activity through the action of more than 20 genes, which also has anti-inflammatory effects; the broccoli seed extract can up-regulate transcription factor Nrf2, and Nrf2 can play a role in increasing highly coordinated antioxidant activity through the action of more than 20 genes. The three components cooperate to achieve good synergistic antioxidation effect.

The health-care food is characterized in that the health-care food is prepared from the components of the health-care food, the components of the health-care food are added with milk-benefiting polypeptide, and the milk-benefiting polypeptide can be matched with pomegranate extracts, fish oil microcapsule powder and onion extracts to play a role in repairing blood vessel intima. In particular, atherosclerosis is induced by endothelial dysfunction that would result directly from insufficient nitric oxide production by endothelial nitric oxide synthase. The pomegranate extract contains various polyphenol compounds, the onion extract contains quercetin and sulfide, and the fish oil microcapsule powder contains omega-3 fatty acid, which can promote the production and secretion of nitric oxide. The functional factor of the milk-benefiting polypeptide is valine-proline tripeptide, which can effectively inhibit the generation of angiotensin, dilate blood vessels, stimulate the intimal cells of the blood vessels to efficiently synthesize nitric oxide, maintain the integrity of the intimal blood vessels, and increase the elasticity of the blood vessels. The components are compatible, so that the tunica intima can be repaired, and the elasticity of blood vessels can be increased.

The proportion of the components of the invention is not set at will, particularly, the main cause of the arteriosclerosis is that the excessive low-density lipoprotein cholesterol leads to direct swallowing and drinking of intima cells, when inflammation is caused, mononuclear cells can phagocytize a large amount of oxidized low-density lipoprotein cholesterol to form foam cells to deposit subcutaneous cells in blood vessels, and fibrous tissue hyperplasia is stimulated to promote the generation of atherosclerosis. A large amount of clinical practice data show that the normal standard reaching rate of low-density lipoprotein cholesterol index can be increased to more than 90% by using 100-200 parts of phytosterol and 10-30 parts of red yeast rice for 1-3 months, and the important reason is that the phytosterol can only achieve the optimal effect within the range of 100-200 parts. Aiming at exogenous cholesterol, the secretion of apolipoprotein B of intestinal tracts and liver cells can be reduced, the absorption of dietary cholesterol and the entrance of bile cholesterol into microbubbles are reduced, the absorption of cholesterol is further reduced, the secretion of bile acid is increased, the reverse transport of cholesterol is improved, a key enzyme (hydroxymethyl glutaryl coenzyme A reductase HMG-COA-R) for synthesizing the cholesterol can be inhibited, and the pathway for synthesizing the cholesterol is blocked. The indexes of the plant sterol are not obviously changed when the plant sterol is lower than 100 parts of low-density lipoprotein cholesterol, the low-density lipoprotein cholesterol is higher than 200 parts of low-density lipoprotein cholesterol, the metabolic burden of the liver is aggravated, the function of the liver is weakened, and the blood sugar of a few people is slightly increased. In fact, the phytosterol has more biological activity only when 100-200 parts, is more suitable for absorption and metabolism of human bodies, and is matched with 10-30 parts of red yeast rice, because the red yeast rice contains a key enzyme (hydroxymethyl glutaryl coenzyme A reductase HMG-COA-R) specific inhibitor for synthesizing the cholesterol by the human bodies, the endogenous pathway for synthesizing the cholesterol can be blocked, and the red yeast rice not only can reduce the total cholesterol, the low-density lipoprotein cholesterol and the arteriosclerosis index, but also can reduce triglyceride and improve the remarkable comprehensive effect of the high-density lipoprotein cholesterol. The ring-opening rate of the monacolin K of the selected red yeast rice is more than 65 percent, the bioavailability of the phytosterol to the liver is greatly improved, and the monacolin K are mutually cooperated and are monarch and minister in the range to exert the best effect on the inhibition of endogenous cholesterol and exogenous cholesterol.

According to the invention, the addition amount of the fish oil microcapsule powder is 100-200 parts, the broccoli seed extract is 2-8 parts, tumor necrosis factor alpha (TNF +) is clinically detected in 1-3 months, and C-reactive protein (CRP) and interleukin 6 (IL-6) are reduced by less than 58%, because the anti-inflammatory effect can only reach 3% when the fish oil microcapsule powder is less than 100 parts, and the anti-inflammatory effect is not substantially changed before when the fish oil microcapsule powder is more than 200 parts, so that omega 3 (EPA and DHA) fatty acid contained in the fish oil microcapsule powder has an obvious influence on mononuclear cell inflammation only when 100-200 parts is used, the optimal effect is achieved, and meanwhile, the broccoli seed extract is 2-8 parts, so that Nrf2 can be adjusted upwards, NF-kB and a series of inflammation media (cell factors, chemotactic factors, adhesion factors, COM-2, MMP-9, iNOS and the like) are reduced, so that the anti-inflammatory effect is achieved, and the activated transcription factor N2 accelerates the transcription of the fish oil microcapsule powder from cell membrane to nucleus to the anti-inflammatory effect, the broccoli seed extract is 2-8 parts, the anti-inflammatory agent, the COM-2, the iNOS and the monarch extract can achieve a synergistic effect when the anti-lipid-reducing effect is in a good range, so that the anti-reducing effect of the anti-inflammatory effect is achieved.

In the formula, 1-5 parts of onion extract, 5-15 parts of mangosteen extract and 5-25 parts of pomegranate extract are used as adjuvant medicines in the range, so that the lipid-lowering and anti-inflammatory effects of the monarch medicine are improved, the antioxidation effect is also realized, the oxidation of low-density lipoprotein cholesterol can be effectively reduced, the phagocytosis of mononuclear cells is reduced, the number of foam cell deposits is reduced, and the occurrence of arteriosclerosis is reduced.

In the formula of the invention, 30-70 parts of nattokinase and 10-40 parts of milk-benefiting polypeptide have the action principle that 30-70 parts of nattokinase can only act on fibrinolysis and platelet aggregation, and simultaneously the blood flow speed is increased, the lipid plaque reduction rate can reach 68.5 percent after the composition is used for 3 months, the plaque dissolving effect is not ideal when the composition is less than 30 parts, and the composition is more than 70 parts of nattokinase can cause diarrhea, vomiting and dyspepsia, and the main reason is that the nattokinase is digested and decomposed by intestinal tracts, and the excessive nattokinase easily stimulates intestines and stomach. The health milk polypeptide 10-40 parts can increase the blood vessel elasticity by more than 50% in 3 months, so that the health milk polypeptide and the health milk polypeptide can be used as a guiding drug together to enable all the natural active plants to better exert respective functions. The compatibility of the whole formula is to effectively intervene by using pure natural food materials according to the pathogenesis of arteriosclerosis and the whole process of arteriosclerosis formation, and meanwhile, the formula has innovative significance for preventing and treating 3 hundred million people's cardiovascular diseases at present.

The broccoli seed extract of the present invention is purchased from Korea-European trade (Shanghai) Co., ltd, or from Brassica corporation, shanghai Heat transfer industry Co., ltd. The content of sulforaphane and sulforaphane glycoside contained in the broccoli seed extract is not less than 13% as a purchase standard at the time of purchase.

Preferably, the pomegranate extract is prepared by the following method: pulverizing pericarpium Granati, drying to obtain powder, extracting with one or more of water, alcohol or other food processing aids, concentrating, and drying to obtain pericarpium Granati extract. Specifically, after the pomegranate peel is crushed and dried, 70% ethanol aqueous solution with the mass ratio of 1. Mixing filtrates, further concentrating under reduced pressure, and vacuum drying to obtain fructus Punicae Granati extract.

Preferably, the mangosteen extract is prepared by the following method: pulverizing mangosteen fruit shell, extracting with ethanol of 70% or more, filtering, centrifuging, concentrating under reduced pressure, and spray drying to obtain mangosteen extract. Specifically, mangosteen nutshell is smashed and then extracted by ethanol with the concentration of more than 70%, wherein the weight ratio of the mangosteen nutshell to the ethanol is 1.

Preferably, the onion extract is prepared by the following method: pulverizing Bulbus Allii Cepae, mixing with ethanol water solution, ultrasonic extracting, filtering, concentrating, centrifuging, and collecting supernatant to obtain Bulbus Allii Cepae extract. Specifically, the onion extract is obtained by taking fresh onions, crushing the onions by a food processor, adding 70% ethanol aqueous solution with the mass ratio of 1 to 10, carrying out ultrasonic extraction for 3 times at 40 ℃, wherein the ultrasonic power is 200W, combining extracting solutions, filtering, carrying out reduced pressure concentration, centrifuging concentrated solution, wherein the centrifugal speed is 4000r/min, the centrifugal time is 10min, and taking supernatant.

It is understood that the pomegranate extract, the mangosteen extract and the onion extract of the present invention can also be obtained commercially. For example, the mangosteen extract is available from Nippon Kabushiki Kaisha, the pomegranate extract is available from Mouthica Biotech, inc., and the onion extract is available from Shaanxi Fuwa Natural products, inc. In addition, the onion extract can be prepared by the extraction method described in JP4348425 or JP 2017-61431. In fact, these extracts, whether obtained from commercial products or extracted by themselves, are of uniform purity and with a certain guarantee, and therefore are all uniform as to the efficacy exerted by the composition after it has been prepared.

The invention also provides a preparation method of the natural plant composition for preventing and treating arteriosclerosis, which comprises the following steps:

pulverizing the above materials respectively, mixing, stirring, and making into preparation.

Preferably, the raw materials are mixed and sieved by a 60-100 mesh sieve.

Preferably, the preparation is any one of tablets, capsules and granules.

The preparation method is simple to operate, and the prepared composition has a good prevention and treatment effect on arteriosclerosis.

The invention also provides application of the natural plant composition for preventing and treating arteriosclerosis in medicines or health-care foods for treating diseases related to arteriosclerosis.

Compared with the prior art, the invention has the following beneficial effects:

the natural plant composition provided by the invention can effectively reduce the content of total cholesterol and low-density lipoprotein cholesterol and prevent platelet aggregation through mutual compatibility of various components, and can play roles in resisting inflammation and oxidation, repairing vascular intima and increasing vascular elasticity, thereby effectively preventing and treating arteriosclerosis.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

Example 1

The following components were weighed: 1000mg of phytosterol, 1000mg of fish oil microcapsule powder, 100mg of milk health polypeptide, 100mg of red yeast rice, 300mg of nattokinase, 10mg of onion extract, 50mg of mangosteen extract, 20mg of broccoli seed extract and 50mg of pomegranate extract.

Respectively pulverizing the above materials, mixing, sieving with 80 mesh sieve, and mixing with multidimensional mixer to obtain tablet with mass of 0.8 g/tablet.

Wherein the Broccoli seed extract is obtained from Korea-European trade (Shanghai) Co., or from Brassica corporation, shanghai Heat transfer industry Co., ltd. The content of sulforaphane and sulforaphane glycoside contained in the broccoli seed extract is not less than 13% as a purchase standard at the time of purchase.

The onion extract is purchased from Shaanxi forest Freund natural products, and can also be directly prepared by adopting the following method: crushing fresh onions by using a food processor, adding 70% ethanol water solution with the mass ratio of 1. In addition, the onion extract can be prepared by the extraction method described in the patent publication No. JP4348425 or the patent publication No. JP 2017-61431.

The mangosteen extract can be purchased from Nippon New drug Co., ltd, or can be directly prepared by the following method: pulverizing mangosteen fruit shell, extracting with ethanol with concentration of 70% or more at 80 deg.C for 1 hr at a weight ratio of 1.

The pomegranate extract can be purchased from Yuanhua Biotech limited, or can be directly prepared by the following method: pulverizing pericarpium Granati, drying, adding 70% ethanol water solution with mass ratio of 1. Mixing filtrates, further concentrating under reduced pressure, and vacuum drying to obtain fructus Punicae Granati extract.

Example 2

The following components were weighed: 1200mg of phytosterol, 1200mg of fish oil microcapsule powder, 200mg of AnYi milk polypeptide, 150mg of red yeast rice, 400mg of nattokinase, 15mg of onion extract, 70mg of mangosteen extract, 30mg of broccoli seed extract and 100mg of pomegranate extract.

Respectively pulverizing the above materials, mixing, sieving with 80 mesh sieve, adding into multidimensional mixer, mixing, and making into tablet with mass of 0.8 g/tablet.

The onion extract, the mangosteen extract, the broccoli seed extract, and the pomegranate extract were obtained in the same manner as in example 1.

Example 3

The following components were weighed: 1500mg of phytosterol, 1500mg of fish oil microcapsule powder, 250mg of milk health polypeptide, 200mg of red yeast rice, 500mg of nattokinase, 20mg of onion extract, 100mg of mangosteen extract, 50mg of broccoli seed extract and 150mg of pomegranate extract.

Example 4

The following components were weighed: 1700mg of phytosterol, 1700mg of fish oil microcapsule powder, 300mg of milk health polypeptide, 250mg of red yeast rice, 600mg of nattokinase, 40mg of onion extract, 120mg of mangosteen extract, 70mg of broccoli seed extract and 200mg of pomegranate extract.

Respectively pulverizing the above materials, mixing, sieving with 60 mesh sieve, and mixing with multidimensional mixer to obtain tablet with mass of 0.8 g/tablet.

Example 5

The following components were weighed: 2000mg of phytosterol, 2000mg of fish oil microcapsule powder, 400mg of AnYi milk polypeptide, 300mg of red yeast rice, 700mg of nattokinase, 50mg of onion extract, 150mg of mangosteen extract, 80mg of broccoli seed extract and 250mg of pomegranate extract.

Respectively pulverizing the above materials, mixing, sieving with 100 mesh sieve, adding into multidimensional mixer, mixing, and making into tablet with mass of 0.8 g/tablet.

Example 6

The specific operation process is the same as that of example 3, except that powder is prepared.

Example 7

The specific operation process is the same as that of example 3, and only capsules are prepared.

Comparative example 1

The specific procedure was as in example 3 except that the phytosterol was added at 2500mg.

Comparative example 2

The specific procedure was the same as in example 3 except that the broccoli seed extract was added in an amount of 100mg.

Comparative example 3

The specific procedure was the same as in example 3 except that the broccoli seed extract was not added.

Comparative example 4

The specific operation process is the same as that of example 3, except that the amount of nattokinase added is 100mg.

Comparative example 5

The specific operation process is the same as that of example 3, except that the addition amount of the fish oil microcapsule powder is 2500mg.

Comparative example 6

The specific operation process is the same as that of example 3, except that the addition amount of the milk benefiting polypeptide is 500mg.

Experimental example 1

The preparations of examples 1 to 7 and comparative examples 1 to 6 were tested for their effects on rat arteriosclerosis as follows: 150 rats were randomly divided into 15 groups of ten. One group was a control group, one group was a model group, and the other three groups were experimental groups. The control group is fed with basic feed, and the model group and the experimental group adopt a method of performing intraperitoneal injection on vitamin D60 ten thousand units/kg once, and simultaneously performing drenching on high-fat feed and 10 mL/kg of fat milk (each 500 mL of fat milk contains 30g of cholesterol, 5 g of sodium cholate, 3g of propylthiouracil and 50g of lard) every day to prepare the arteriosclerosis model.

The experimental group was administered with 0.2 g/(kg. D) of the formulations of examples 1 to 7 and comparative examples 1 to 6, respectively, and the control group and the model group were administered with the same amount of distilled water per day. The blood lipid, TNF-alpha, IL-6 and NF-kB are measured by taking blood from the ocular venous plexus 1 time per day for 60 days continuously after the last administration for 2 hours, and the abdominal aorta hemorheology index is measured. The rat is killed, abdominal aorta, heart, liver and the like are taken and soaked in 10% formalin, the pathological changes of the artery, the heart and the liver are observed through routine paraffin embedding, slicing and HE staining, the ratio of the mesolamella thickness to the radius of the inner cavity of the blood vessel is calculated, the positive expression of the mesolamella NF-kB in the artery is observed through immunohistochemical analysis by adopting an SABC method, and the pathological changes of the artery are observed.

The pathological morphological observation result shows that the control rat has clear vascular structure, complete internal elastic facial mask and regular arrangement of the smooth muscle cells of the mesolamella, and is parallel to the internal elastic facial mask. The aorta of the rat in the model group has atherosclerotic plaque, the elastic membrane in the blood vessel is damaged and broken, the tunica media is obviously irregularly thickened, the ratio of the thickness of the tunica media to the radius of the lumen of the blood vessel is obviously increased, the arrangement of smooth muscle cells is disordered, the cells tend to migrate to the tunica intima, and the smooth muscle fibers are obviously disordered; the lipid deposition of myocardial tissues is severe, and a large number of red-stained particles appear in cytoplasm of myocardial cells in a lesion area; liver sections were strongly positive by oil red O staining. Compared with the model group, the breakage of the elastic membrane in the blood vessel of rats in each experimental group is reduced, partial stretching is flat, the arrangement of the fibers of the smooth muscle of the mesolamella is slightly disordered, and compared with the effect of the example 3 and the comparative example 6, the repairing effect of the example 3 on the elastic membrane is not much different from that of the comparative example 6, which shows that the repairing effect cannot be improved by continuously increasing the amount of the milk polypeptide for benefiting health after the amount reaches a certain amount.

The lowering effect and anti-inflammatory effect on total cholesterol and low density lipoprotein cholesterol levels are shown in table 1 below:

TABLE 1

As can be seen from Table 1, the natural plant composition of the present invention can significantly reduce the total cholesterol and low density lipoprotein cholesterol levels and has significant anti-inflammatory effects. Comparing example 3 with comparative example 1, it can be seen that the results of example 3 and comparative example 1 are not very different in the performance of reducing the total cholesterol and low density lipoprotein cholesterol levels, and the effect of example 3 is slightly better than that of comparative example 1, probably because the excessive phytosterol is difficult to be absorbed by human body, and the synergistic promotion effect of red yeast rice on phytosterol is inhibited to a certain extent, which shows that the optimal effect of reducing the total cholesterol and low density lipoprotein cholesterol levels can be achieved only when the ratio of phytosterol to red yeast rice is a certain ratio.

Comparing the anti-inflammatory effects of example 3 with those of comparative examples 2, 3 and 5, it can be seen that the anti-inflammatory effect of example 3 is slightly superior to that of comparative examples 2 and 5, and is significantly superior to that of comparative example 3. The reason is that the broccoli seed extract and the fish oil microcapsule powder can mutually cooperate to achieve good anti-inflammatory effect, and the anti-inflammatory effect can be optimized only when the mass ratio of the broccoli seed extract to the fish oil microcapsule powder is maintained to be a certain ratio.

The effect on platelet aggregation in rats is shown in table 2 below:

TABLE 2

As can be seen from Table 2, the natural plant composition of the present invention can significantly inhibit the aggregation of platelets. Comparing example 3 with comparative example 4, it can be seen that in the case where the amount of nattokinase added is not as much as in comparative example 4, the platelet aggregation preventing effect of example 3 is slightly better than that of comparative example 4, probably because the excessive amount of nattokinase inhibits the synergistic effect with the onion extract to some extent, indicating that the platelet aggregation preventing effect is optimal only in the case where the two are in a certain ratio.

Experimental example 2

21 patients with arteriosclerosis were treated with the natural plant composition prepared in example 3, and serum was measured after a certain period of treatment, and the results are shown in Table 3.

TABLE 3

As can be seen from the above table, the natural plant composition of the present invention has a very good therapeutic effect on the significant reduction of total cholesterol and low density lipoprotein cholesterol, thereby having very good preventive and therapeutic effects on the prevention and recovery of arteriosclerosis.

Typical cases

Case 1: when a woman is 63 years old, CT shows that the liver is occupied and damaged, and regular examination is required. After taking the product for 1 month, the liver is rechecked to only leave uneven fatty liver, and other indexes are restored to normal.

Case 2: some Panzhi, male, 57 years old, the left common carotid artery intersection has plaque generation after blood vessel examination, the right side 1863 of the arteriosclerosis degree, the left side 1832, the product is taken for 3 months and then the double examination is carried out, the intima in the two common carotid arteries is thickened, the plaque disappears, the right side 1723 of the arteriosclerosis degree, and the left side 1667.

Case 3: for a certain woman in 53 years old with hyperlipidemia, the American direct marketing brand Yossana is taken for a long time, but the blood lipid is not obviously improved. After the product is taken for one month, the blood fat is recovered to be normal, and after the product is taken for three months, the liver index is recovered to be normal.

Case 4: some Hu, male, age 64, sudden cerebral infarction, inconvenient movement after discharge, unclear speaking and vomiting, edema of hands and feet on the affected side of hemiplegia and grade 3 grip strength. The hand and foot edema disappears after half a year of using the product, and the user can walk by himself.

Case 5: a certain horse, male, 62 years old, has been examined by physical examination to find subacute cerebral infarction, old cerebral infarction, cerebral white matter demyelination, essential hypertension, dyslipidemia, bilateral anterior cerebral artery A1 with far occlusion, internal carotid plaque formation and arteriosclerosis, left common carotid artery thickness 1.5mm, bilateral vertebral artery left 3.6mm, right 2.9mm, right femoral artery 2.0mm, left femoral artery 2.8mm, left common carotid artery thickness 1.1mm, bilateral vertebral artery left 3.5mm, right 3.0mm, right femoral artery 1.4mm, left femoral artery 2.3mm after half a year of conditioning by the product.

While particular embodiments of the present invention have been illustrated and described, it would be obvious that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.

Claims

1. A natural plant composition for preventing and treating arteriosclerosis is characterized by comprising the following components in parts by mass: 100-200 parts of phytosterol, 100-200 parts of fish oil microcapsule powder, 10-40 parts of milk-benefiting polypeptide, 10-30 parts of red yeast rice, 30-70 parts of nattokinase, 1-5 parts of onion extract, 5-15 parts of mangosteen extract, 2-8 parts of broccoli seed extract and 5-25 parts of pomegranate extract;

the pomegranate extract is prepared by the following method: pulverizing pericarpium Granati, drying to obtain powder, extracting with one or more of water, alcohol or other food processing aids, concentrating, and drying to obtain pericarpium Granati extract;

the mangosteen extract is prepared by adopting the following method: pulverizing mangosteen fruit shell, extracting with ethanol of 70% or more, filtering, centrifuging, concentrating under reduced pressure, and spray drying to obtain mangosteen extract;

the onion extract is prepared by the following method: pulverizing Bulbus Allii Cepae, mixing with ethanol water solution, ultrasonic extracting, filtering, concentrating, centrifuging, and collecting supernatant to obtain Bulbus Allii Cepae extract.

2. The natural plant composition for preventing and treating arteriosclerosis as set forth in claim 1, wherein the plant sterol is 120-170 parts, the fish oil microcapsule powder is 120-170 parts, the milk's galangal polypeptide is 20-30 parts, the red yeast rice is 15-25 parts, the nattokinase is 40-60 parts, the onion extract is 1.5-4 parts, the mangosteen extract is 7-12 parts, the broccoli seed extract is 3-7 parts, and the pomegranate extract is 10-20 parts.

3. The natural plant composition for preventing and treating arteriosclerosis as defined in claim 1, wherein the plant sterol is 150 parts, the fish oil microcapsule powder is 150 parts, the milk polypeptide is 25 parts, the red yeast rice is 20 parts, the nattokinase is 50 parts, the onion extract is 2 parts, the mangosteen extract is 10 parts, the broccoli seed extract is 5 parts, and the pomegranate extract is 15 parts.

4. A method for preparing the natural plant composition for the prevention and treatment of arteriosclerosis as defined in any one of claims 1-3, comprising the steps of:

5. The method of claim 4, wherein the raw materials are mixed and then screened through a 60-100 mesh screen.

6. The method of claim 4, wherein the preparation is any one of a tablet, a capsule and a granule.