CN114982555A - Culture medium suitable for Dictyophora rubrovalvata cultivars and using and production method thereof - Google Patents
Culture medium suitable for Dictyophora rubrovalvata cultivars and using and production method thereof Download PDFInfo
- Publication number
- CN114982555A CN114982555A CN202210803489.0A CN202210803489A CN114982555A CN 114982555 A CN114982555 A CN 114982555A CN 202210803489 A CN202210803489 A CN 202210803489A CN 114982555 A CN114982555 A CN 114982555A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- cultivars
- beet pulp
- culture
- inoculation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000001963 growth medium Substances 0.000 title claims abstract description 45
- 241001313734 Dictyophora Species 0.000 title claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 title abstract description 17
- 235000016068 Berberis vulgaris Nutrition 0.000 claims abstract description 24
- 241000335053 Beta vulgaris Species 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 229910001868 water Inorganic materials 0.000 claims abstract description 21
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 20
- 239000000126 substance Substances 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 13
- 235000018185 Betula X alpestris Nutrition 0.000 claims abstract description 10
- 235000018212 Betula X uliginosa Nutrition 0.000 claims abstract description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 10
- 229930006000 Sucrose Natural products 0.000 claims abstract description 10
- 239000010440 gypsum Substances 0.000 claims abstract description 10
- 229910052602 gypsum Inorganic materials 0.000 claims abstract description 10
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 10
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 10
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 10
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 10
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 10
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 10
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 10
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 3
- 244000068988 Glycine max Species 0.000 claims abstract description 3
- 238000011081 inoculation Methods 0.000 claims description 21
- 239000000463 material Substances 0.000 claims description 20
- 241000894007 species Species 0.000 claims description 15
- 241000233866 Fungi Species 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 14
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 241000890685 Dictyophora rubrovolvata Species 0.000 claims description 11
- 235000019764 Soybean Meal Nutrition 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 239000004455 soybean meal Substances 0.000 claims description 7
- 238000011049 filling Methods 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 238000004080 punching Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000007580 dry-mixing Methods 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 3
- 239000000306 component Substances 0.000 abstract description 15
- 239000011521 glass Substances 0.000 abstract description 10
- 230000009467 reduction Effects 0.000 abstract description 2
- 239000002361 compost Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000012257 stirred material Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to a culture medium suitable for Dictyophora rubrovalvata cultivars and a using and production method thereof; the culture medium comprises the following components: birch sawdust, wheat bran powder, soybean powder, beet pulp, white sugar, gypsum, monopotassium phosphate, magnesium sulfate and water. After the culture medium substance and the culture medium components are used, the growth period of the culture medium substance and the culture medium components is shortened by 30-40 days by combining the production method compared with that of the traditional glass bottle culture seeds, and the pollution rate is greatly reduced due to the reduction of the growth period.
Description
Technical Field
The invention belongs to the technical field of plant production, and particularly relates to a culture medium suitable for Dictyophora rubrovalvata cultivars and the technical field of a using and production method.
Background
The existing cultivars are divided into two types, glass bottle cultivars and lantern ring cultivars. The conventional lantern ring cultivation mode needs a lantern ring process, manual operation is involved more, and fungus pollution is easily caused. The traditional glass bottle cultivation seeds can only contain 1 jin of cultivation material and can only be manually filled, so that the nutrient supply for the growth of hypha in the later period is insufficient, and new nutrient components are not easily supplemented. The process of supplementing new nutrient components in time faces the defects of secondary pollution, time waste and labor waste. Therefore, the manual operation is reduced, a proper culture medium substance is found, the production and the use of enough hypha are realized by filling once, and the complexity and the risk of filling after the filling are saved become technical problems to be solved.
Disclosure of Invention
The invention aims to solve the defects of the problems and is suitable for the culture medium of Dictyophora rubrovolvata cultivated species and the using and production method. The invention has the advantages that lantern rings are not needed, the labor input is greatly reduced, a specially-made bagging machine can be adopted for bagging operation, the manufacturing speed is greatly improved, the pollution rate of fungus sticks can be effectively reduced after labor is reduced, and the like. In particular, the formula of the invention increases beet pulp compared with the auxiliary materials required by the traditional formula, so that the water content is easier to preserve and the spawn running speed is improved compared with the traditional formula.
The invention is realized by adopting the following technical scheme.
The invention relates to a culture medium substance suitable for Dictyophora rubrovalvata cultivars, which is beet pulp.
The weight of the beet pulp accounts for 2-10% of the weight of the whole culture medium.
The weight of the beet pulp accounts for 2-4% of the weight of the whole culture medium.
The weight of the beet pulp accounts for 5-10% of the weight of the whole culture medium.
The culture medium using the substances comprises the following components: birch sawdust, wheat bran powder, soybean powder, beet pulp, white sugar, gypsum, monopotassium phosphate, magnesium sulfate and water.
The culture medium of the invention comprises the following components by weight: 70-80% of birch sawdust, 5-15% of wheat bran powder, 2-5% of soybean meal, 2-10% of beet pulp, 0.5-2% of white sugar, 1-2% of gypsum, 0.1-0.3% of monopotassium phosphate and 0.1-0.2% of magnesium sulfate; the water content is controlled to be 60-70%.
The culture medium of the invention comprises the following components by weight: 80-85% of birch sawdust, 5-10% of wheat bran powder, 2-5% of soybean meal, 2-4% of beet pulp, 0.5-2% of white sugar, 1-2% of gypsum, 0.1-0.3% of monopotassium phosphate and 0.1-0.2% of magnesium sulfate; the water content is controlled to be 60-70%.
The culture medium of the invention comprises the following components by weight: 70-80% of birch sawdust, 10-15% of wheat bran powder, 2-5% of soybean meal, 5-10% of beet pulp, 0.5-2% of white sugar, 1-2% of gypsum, 0.1-0.3% of monopotassium phosphate and 0.1-0.2% of magnesium sulfate; the water content is controlled to be 60-70%.
The method for producing Dictyophora rubrovalvata cultivars by using the culture medium comprises the following steps of:
step 1), preparing a culture medium material required by dictyophora rubrovolvata cultivars;
step 2) calculating the use amount of each component of the produced cultivar culture medium, diluting soluble substances by using a small basin, pouring the diluted solution into a container filled with 80 percent of the calculated water amount for secondary dilution, uniformly scattering insoluble substances into a stirrer for dry mixing for 20 minutes, and finally adding the diluted solution into the dry mixed material for stirring for 30 minutes;
step 3) uniformly mixing the culture materials, and filling the mixture into cultivation bags with the specification of 16cm multiplied by 35cm multiplied by 5cm by a bagging machine;
step 4), centralizing and sterilizing the cultivated species filled with the materials;
step 5) sterilizing the inoculation tool, wherein the whole inoculation environment is carried out in a sterile environment;
step 6) breaking the selected stock seed bottles with high hypha purity and good vitality, pulling out the punching rod of the cultivated species, clamping three pieces of edible finger-shaped stock seeds by using forceps, inoculating the edible finger-shaped stock seeds in the holes of the cultivated species by a three-point inoculation method of over, middle and lower parts, and sealing the holes by using sponges after inoculation;
and 7) uniformly placing the inoculated cultivated species in a culture room for mother culture.
The step 3) of the invention comprises the following steps: the tightness is moderate, and the standard tightness is not loose and compact and has a rod-shaped feeling when being pinched by hands; secondly, controlling the bagging time, and filling the compost into the bags for no more than 4 hours; and thirdly, when the bag is packed, the middle of the fungus stick is perforated by the perforating stick and then sealed, and a later inoculation space is reserved.
The step 3) of the invention comprises the following steps: a sterilization step: firstly, the sterilizer must reach more than 100 ℃ within 1 hour;
secondly, the high pressure lasts for 3 hours at 121 ℃, and the normal pressure lasts for 11 hours at more than 100 ℃;
and thirdly, after sterilization, naturally reducing the temperature in the sterilizer to 60 ℃, slowly opening a sterilizer door to 20-30 cm to prevent the outside cold air from adsorbing bacteria sticks to form water drops, and after one hour, carrying the original seed bottles to a cooling chamber to be cooled to about 20 ℃ and then carrying out next inoculation work.
The method has the beneficial effects that the problems of high cost, small strain quantity and complex production procedure of the traditional Dictyophora rubrovalvata glass bottle cultivation seed are solved. The invention can replace the glass bottle with the oiled paper, thereby effectively reducing the production cost; the traditional glass bottle cultivation seeds can only contain 1 jin of cultivation material, the new cultivation seeds can reach 2.2 jin/rod, the hypha growth nutrition supply is sufficient in the later period, and the Dictyophora rubrovalvata yield is obviously improved; the traditional glass bottle charging can be only manually carried out, and the novel cultivar production method can be carried out by using a bagging machine, so that the production speed is greatly improved, and a large amount of labor cost is reduced; the three-point inoculation method fully utilizes the reserved holes, and the hyphae are inoculated on the upper, middle and lower three points of the fungus stick, so that the growth speed of the hyphae can be greatly improved. After the culture medium substance and the culture medium components are used, the growth period of the culture medium substance and the culture medium components is shortened by 30-40 days by combining with a production method compared with that of the traditional glass bottle culture seeds, and the pollution rate is greatly reduced due to the reduction of the growth period.
The invention is further explained below with reference to the drawings and the detailed description.
Drawings
FIG. 1 is a diagram of a product of the present invention.
FIG. 2 is a pictorial view of a control product.
Detailed Description
See fig. 1 and fig. 2. Fig. 1 is a photograph of a dictyophora rubrovolvata entity produced by the formula 1 of the invention. Fig. 2 is a control product: photograph of a real object of formulation 3.
The specific production method comprises the following steps:
1) preparing culture medium materials required by production of dictyophora rubrovolvata cultivars, wherein the culture medium materials comprise the following components in percentage by mass/weight: 70-80% of birch sawdust, 10-15% of wheat bran powder, 2-5% of soybean meal, 5-10% of beet pulp, 0.5-2% of white sugar, 1-2% of gypsum, 0.1-0.3% of monopotassium phosphate and 0.1-0.2% of magnesium sulfate; the water content of the culture medium of the cultivar is controlled to be 60-70%.
Table 1 three groups of formulation ingredient tables for experiments of the invention (mass percentage)
Compared with the traditional formula, the formula is added with the beet pulp, can provide sufficient sugar for the dictyophora rubrovolvata, has good water binding capacity, and can ensure that the dictyophora rubrovolvata can keep the residual water required by growth and development during soil covering planting. In the formula, the reduced beet pulp component is filled by sawdust.
The formula 1 is added with 5-10% of beet pulp compared with the traditional formula, experimental comparison is carried out, the beet pulp is added into the experimental group 1, 2-5% of beet pulp is added into the experimental group 2, the beet pulp is not added into the experimental group 3, the planting procedure is controlled to be consistent, the watering amount is controlled to be consistent, and the yield of the experimental group 1 is 10% higher than that of the experimental group 2 and 20% higher than that of the experimental group 3;
the moisture of the fungus sticks in the same batch in the culture room is regularly measured, and the moisture of the fungus sticks in the experimental group 1 is found to be higher than that of the fungus sticks in the experimental group 2 and the experimental group 3, and the growth speed of hyphae is 2-5% faster than that of the fungus sticks in the experimental group 2 and 5-10% faster than that of the fungus sticks in the experimental group 3.
2) Calculating the use amount of each component of the culture medium for producing the cultivated species, diluting the soluble substance by using a small basin, pouring the diluted solution into a container filled with 80% of the calculated water amount for secondary dilution, uniformly scattering the insoluble substance into a stirrer for dry stirring for 20 minutes, finally adding the diluted solution into the dry stirred material for stirring for 30 minutes, and properly supplementing water when the water content is not enough;
3) the culture materials are uniformly mixed, the mixture is packaged into a cultivation bag with the specification of 16cm multiplied by 35cm multiplied by 5cm by a bagging machine (the cultivation bag with the size is easy to operate, the weight of a single fungus stick is controlled to be the maximum limit capable of being operated by one hand of an adult, the sufficient supply of nutrition required by the late growth of the dictyophora rubrovolvata can be ensured by loading more materials), and the following points are required to be paid attention when the cultivation bag is packaged:
the tightness is moderate, and the standard tightness is good in the sense of no looseness, compactness and rod shape when pinched by hands.
Secondly, the bagging time is controlled, the compost is placed into the bag, the temperature of the compost rises very quickly due to air impermeability, and the bagging time is preferably not more than 4 hours from the beginning to the end in order to prevent the fermentation of the culture medium. And thirdly, when the bag is packed, the middle of the fungus stick is perforated by the perforating stick and then sealed, and a later inoculation space is reserved. (the hole is punched in the middle of the cultivation material by the punching rod, so that not only is the subsequent inoculation operation convenient, but also the reserved hole can provide sufficient oxygen supply for hyphae, and the growth speed of the hyphae is 30 to 40 percent higher than that of the hyphae of a glass bottle cultivation seed)
Fourthly, bagging environment: the bag is required to be bagged in a ventilated, cool, low-temperature and dark and direct-lighting place.
4) And (3) centralizing and sterilizing the cultivated species filled with the materials in a unified way, wherein the sterilization requirements are as follows:
the sterilizer must reach over 100 deg.C within 1 hour.
② the high pressure lasts 3 hours at 121 ℃, and the normal pressure lasts 11 hours at more than 100 ℃.
And thirdly, after sterilization, naturally reducing the temperature in the sterilizer to 60 ℃, slowly opening a sterilizer door to 20-30 cm (preventing outside cold air from adsorbing bacteria sticks to form water drops), and after one hour, carrying the original seed bottles to a cooling chamber to be cooled to about 20 ℃ and then carrying out next inoculation work.
5) And (4) sterilizing the inoculation tool, wherein the whole inoculation environment is in an aseptic environment.
6) Breaking the selected stock bottle with high hypha purity and good activity, pulling out the perforating rod of the cultivated species, clamping three edible finger-shaped stock seeds with forceps, inoculating in the holes of the cultivated species by a three-point inoculation method of more than three edible finger-shaped stock seeds, middle and lower, and sealing with sponge after inoculation.
(three-point inoculation method fully utilizes the reserved holes, hypha is inoculated on the upper, middle and lower three points of the fungus stick, the culture speed of the fungus stick can be greatly improved, the growth period is shortened by 30-40 days compared with the traditional glass bottle culture seeds, and the pollution rate is greatly reduced due to the shortening of the growth period)
7) Uniformly placing the inoculated cultivated species in a culture room for mother species culture.
The above description is only a part of specific embodiments of the present invention (since the formula of the present invention includes numerical ranges, the embodiments are not exhaustive, the protection scope of the present invention includes the numerical ranges and other technical point ranges), and the specific contents or common knowledge in the schemes are not described too much. It should be noted that the above-mentioned embodiments do not limit the present invention in any way, and all technical solutions obtained by means of equivalent substitution or equivalent transformation for those skilled in the art are within the protection scope of the present invention. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. A culture medium substance suitable for Dictyophora rubrovalvata cultivars is characterized in that the culture medium substance is beet pulp.
2. The culture medium material of novel Dictyophora rubrovolvata cultivars according to claim 1, wherein the weight of beet pulp is 2-10% of the total weight of the culture medium.
3. The culture medium material of novel Dictyophora rubrovolvata cultivars according to claim 1, wherein the weight of beet pulp is 5-10% of the total weight of the culture medium.
4. A medium for use with the substance of claim 1, wherein the medium comprises the following components: birch sawdust, wheat bran powder, soybean powder, beet pulp, white sugar, gypsum, monopotassium phosphate, magnesium sulfate and water.
5. The culture medium according to claim 4, wherein the culture medium comprises, by weight: 70-80% of birch sawdust, 5-15% of wheat bran powder, 2-5% of soybean meal, 2-10% of beet pulp, 0.5-2% of white sugar, 1-2% of gypsum, 0.1-0.3% of monopotassium phosphate and 0.1-0.2% of magnesium sulfate; the water content is controlled to be 60-70%.
6. The culture medium according to claim 1, wherein the culture medium comprises, by weight: 80-85% of birch sawdust, 5-10% of wheat bran powder, 2-5% of soybean meal, 2-4% of beet pulp, 0.5-2% of white sugar, 1-2% of gypsum, 0.1-0.3% of monopotassium phosphate and 0.1-0.2% of magnesium sulfate; the water content is controlled to be 60-70%.
7. The culture medium according to claim 1, wherein the culture medium comprises, by weight: 70-80% of birch sawdust, 10-15% of wheat bran powder, 2-5% of soybean meal, 5-10% of beet pulp, 0.5-2% of white sugar, 1-2% of gypsum, 0.1-0.3% of monopotassium phosphate and 0.1-0.2% of magnesium sulfate; the water content is controlled to be 60-70%.
8. A process for producing Dictyophora rubrovolvata cultivars using the medium according to any one of claims 4 to 7, characterized in that said process comprises the steps of:
step 1), preparing a culture medium material required by dictyophora rubrovolvata cultivars;
step 2) calculating the use amount of each component of the produced cultivar culture medium, diluting soluble substances by using a small basin, pouring the diluted solution into a container filled with 80 percent of the calculated water amount for secondary dilution, uniformly scattering insoluble substances into a stirrer for dry mixing for 20 minutes, and finally adding the diluted solution into the dry mixed material for stirring for 30 minutes;
step 3) uniformly mixing the culture materials, and filling the mixture into cultivation bags with the specification of 16cm multiplied by 35cm multiplied by 5cm by a bagging machine;
step 4), centralizing and sterilizing the cultivated species filled with the materials;
step 5) sterilizing the inoculation tool, wherein the whole inoculation environment is in an aseptic environment;
step 6) breaking the selected stock seed bottles with high hypha purity and good vitality, pulling out the punching rod of the cultivated species, clamping three pieces of edible finger-shaped stock seeds by using forceps, inoculating the edible finger-shaped stock seeds in the holes of the cultivated species by a three-point inoculation method of over, middle and lower parts, and sealing the holes by using sponges after inoculation;
and 7) uniformly placing the inoculated cultivated species in a culture room for mother culture.
9. The method of claim 8, wherein said step 3) comprises: the tightness is moderate, and the standard tightness is not loose and compact and has a rod-shaped feeling when being pinched by hands; secondly, the bagging time is controlled, and the culture materials are placed into the bags for no more than 4 hours; and thirdly, when the bag is packed, the middle of the fungus stick is perforated by the perforating stick and then sealed, and a later inoculation space is reserved.
10. The method of claim 8, wherein the step 3) comprises: a sterilization step: firstly, the sterilizer must reach more than 100 ℃ within 1 hour;
secondly, the high pressure lasts for 3 hours at 121 ℃, and the normal pressure lasts for 11 hours at more than 100 ℃;
and thirdly, after sterilization, naturally reducing the temperature in the sterilizer to 60 ℃, slowly opening a sterilizer door to 20-30 cm to prevent the outside cold air from adsorbing bacteria sticks to form water drops, and after one hour, carrying the original seed bottles to a cooling chamber to be cooled to about 20 ℃ and then carrying out next inoculation work.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210803489.0A CN114982555A (en) | 2022-07-07 | 2022-07-07 | Culture medium suitable for Dictyophora rubrovalvata cultivars and using and production method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210803489.0A CN114982555A (en) | 2022-07-07 | 2022-07-07 | Culture medium suitable for Dictyophora rubrovalvata cultivars and using and production method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114982555A true CN114982555A (en) | 2022-09-02 |
Family
ID=83020240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210803489.0A Pending CN114982555A (en) | 2022-07-07 | 2022-07-07 | Culture medium suitable for Dictyophora rubrovalvata cultivars and using and production method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114982555A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017219394A1 (en) * | 2016-06-24 | 2017-12-28 | 四川省大真科技有限责任公司 | Seed production method of cultivated species of edible fungus and cultivation method of cultivated species and edible fungus prepared thereby |
| CN108040747A (en) * | 2017-11-13 | 2018-05-18 | 邱汪洋 | A kind of dictyophora phalloidea compost |
| CN110100657A (en) * | 2019-06-03 | 2019-08-09 | 贵州织金毫米农业科技发展有限公司 | A kind of production method of Dictyophora rubrovalvata test tube stock |
| CN110651668A (en) * | 2018-06-28 | 2020-01-07 | 贵州金蟾大山生物科技有限责任公司 | Dictyophora rubrovalvata three-point bagging inoculation method |
-
2022
- 2022-07-07 CN CN202210803489.0A patent/CN114982555A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017219394A1 (en) * | 2016-06-24 | 2017-12-28 | 四川省大真科技有限责任公司 | Seed production method of cultivated species of edible fungus and cultivation method of cultivated species and edible fungus prepared thereby |
| CN108040747A (en) * | 2017-11-13 | 2018-05-18 | 邱汪洋 | A kind of dictyophora phalloidea compost |
| CN110651668A (en) * | 2018-06-28 | 2020-01-07 | 贵州金蟾大山生物科技有限责任公司 | Dictyophora rubrovalvata three-point bagging inoculation method |
| CN110100657A (en) * | 2019-06-03 | 2019-08-09 | 贵州织金毫米农业科技发展有限公司 | A kind of production method of Dictyophora rubrovalvata test tube stock |
Non-Patent Citations (1)
| Title |
|---|
| 孔祥君等: "食用菌生产技术问答", vol. 1, 31 October 1986, 上海科学技术出版社, pages: 211 - 212 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103891524B (en) | The method of glossy ganoderma dish garden formula cultivation and the medium for cultivating ganoderma | |
| CN102845219B (en) | Cultivation technique of Pleurotus eryngii | |
| CN103355094A (en) | Method of cultivating tremella by utilizing mulberry twig | |
| CS237990A3 (en) | Process for cultivating tree dry-rot edible fungi | |
| CN105900690A (en) | Pleurotus ostreatus cultivation method | |
| CN107736184A (en) | A kind of Pleurotus eryngii industrial cultivation method based on high activity liquid spawn | |
| CN104761386B (en) | The compost and preparation method of a kind of utilization giant knotweed rhizome residue Xinbao mushroom culturing | |
| CN102742452A (en) | Manufacture method of corncob halimasch production seeds | |
| CN111386964A (en) | Tremella aurantialba liquefied strain inoculation culture method | |
| CN107266227A (en) | A kind of edible fungus culturing matrix and preparation method thereof | |
| CN102948324A (en) | Method for producing edible mushroom by used biological packing | |
| CN107135805A (en) | A kind of asparagus ecology planting method based on high nutrition | |
| CN103907477A (en) | Method and medium for cultivating mushrooms in potted landscape manner | |
| CN109566268A (en) | Effective original seed of a kind of gold ear and preparation method thereof | |
| CN108293592A (en) | A method of cultivating needle mushroom using sorghum flour mixture | |
| CN105519345B (en) | The gastrodia elata sexual propagation method that cotton seed hulls is that major ingredient makes Germination Strain production kind | |
| CN107980474A (en) | A kind of cultural method of needle mushroom | |
| CN111386963A (en) | Tremella aurantialba cultivation method using tremella aurantialba seedling inoculation | |
| CN105272473A (en) | Formula and method for cultivating tremella through pleurotus eryngii waste substrates | |
| CN107384824A (en) | Stalk rotten agent soon | |
| CN109168957A (en) | A kind of high-yield flammulina velutipes culture medium matter and its cultural method | |
| CN114982555A (en) | Culture medium suitable for Dictyophora rubrovalvata cultivars and using and production method thereof | |
| CN106045729A (en) | Blueberry seedling cultivation substrate and preparation method thereof | |
| CN107182782B (en) | A method of accelerating sunflower generation process | |
| CN110402761A (en) | A kind of Pleurotus eryngii culture medium and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |