CN109168957A - A kind of high-yield flammulina velutipes culture medium matter and its cultural method - Google Patents
A kind of high-yield flammulina velutipes culture medium matter and its cultural method Download PDFInfo
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- CN109168957A CN109168957A CN201811174317.1A CN201811174317A CN109168957A CN 109168957 A CN109168957 A CN 109168957A CN 201811174317 A CN201811174317 A CN 201811174317A CN 109168957 A CN109168957 A CN 109168957A
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- flammulina velutipes
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- needle mushroom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention discloses a kind of high-yield flammulina velutipes culture medium matter, are made of the component of following parts by weight: 30-40 parts of obsolete fungus stick, 20-25 parts of cornstarch, 6-11 parts of poultry-dung, 7-13 parts of rice husk, 6-10 parts of nano diatomite, 8-15 parts of Cortex sophorae, 5-12 parts of rapeseed dregs of rice, 1-3 parts of rock phosphate powder, 1-2 parts of plant ash, 1-5 parts of beans stalk crumb, vitamin B20.3-0.6 parts, 4-6 parts of potassium superphosphate, 0.4-0.7 parts of lanthana, 0.5-0.8 parts of cerium oxide, 0.06-0.08 parts of gamma-polyglutamic acid, 8-14 parts of tea grounds, 1-5 parts of nutritional additive, 2-4 parts of precipitated calcium carbonate, culture medium for golden mushroom matter of the invention, its raw material passes through rational proportion, also add nutritional additive, comprehensive nutrition is provided for Growth of Flammulina Velutipes, the disease-resistant and anti-miscellaneous bacteria infection ability of needle mushroom is also improved simultaneously, the needle mushroom quality and yield planted are high.
Description
Technical field
The present invention relates to technical field of edible fungi cultivation, specifically a kind of high-yield flammulina velutipes culture medium matter and its cultivation side
Method.
Background technique
Needle mushroom is because its stem is elongated, like day lily, therefore claims needle mushroom.Needle mushroom is the edible of autumn and winter and early spring cultivation
Bacterium, with its cap it is sliding it is tender, handle is crisp, full of nutrition, tastes delicious and be world-famous for, full of nutrition, A sweety scent assails the nostrils and taste
It is delicious, it is deeply loved by the public.According to surveying and determination, the content of needle mushroom amino acid is very rich, is higher than general mushroom class, especially relies ammonia
The content of acid is especially high, and lysine has the function of promoting children's intelligence development.Contain protein 8.87%, carbon in needle mushroom dry product
Hydrate 60.2%, crude fibre are often edible to prevent and treat canker up to 7.4%.A kind of contained substance has very in needle mushroom
Good antitumaous effect.Needle mushroom is both a kind of ticbit and preferable health food, and the domestic and international market of needle mushroom is increasingly
It is wide.
The key of needle mushroom artificial cultivation technique is the collocation of compost at present.Compost is that edible mushroom depends on for existence
The source of various nutriments.Nutriment required for edible fungi growth generally has carbon nutrition, nitrogen nutrition, dimension life
Element and minerals etc..The ingredient of composition edible mushroom culture matter is broadly divided into major ingredient and auxiliary material.Major ingredient, which refers to, accounts for clinker in compost
Heavy carbon nutrition substance, auxiliary material refer to that ingredient is less, nitrogen content is higher in compost, the carbon for adjusting compost
The substance of nitrogen ratio.In golden mushroom plantation, traditional major ingredient has cottonseed, weed tree sawdust, crop material etc., and traditional auxiliary material has wheat bran, rice
Chaff, corn flour, soy meal etc..
There are many method of needle mushroom artificial cultivation, but since needle mushroom compost type is more single, higher cost, culture
Material nutrient collocation is unreasonable and environment temperature control is bad, and production management lacks scientific and effective method, current needle mushroom
Generally existing low yield, the disadvantages of growth cycle is long.
Summary of the invention
The purpose of the present invention is to provide a kind of high-yield flammulina velutipes culture medium matter and its cultural methods, to solve above-mentioned background
The problem of being proposed in technology.
To achieve the above object, the invention provides the following technical scheme:
A kind of high-yield flammulina velutipes culture medium matter, is made of the component of following parts by weight:
30-40 parts of obsolete fungus stick, 20-25 parts of cornstarch, 6-11 parts of poultry-dung, 7-13 parts of rice husk, nano diatomite 6-
10 parts, 8-15 parts of Cortex sophorae, 5-12 parts of rapeseed dregs of rice, 1-3 parts of rock phosphate powder, 1-2 parts of plant ash, 1-5 parts of beans stalk crumb, vitamin
B20.3-0.6 parts, 4-6 parts of potassium superphosphate, 0.4-0.7 parts of lanthana, 0.5-0.8 parts of cerium oxide, gamma-polyglutamic acid 0.06-
0.08 part, 8-14 parts of tea grounds, 1-5 parts of nutritional additive, 2-4 parts of precipitated calcium carbonate.
A kind of high-yield flammulina velutipes culture medium matter, is made of the component of following parts by weight:
30 parts of obsolete fungus stick, 20 parts of cornstarch, 6 parts of poultry-dung, 7 parts of rice husk, 6 parts of nano diatomite, 8 parts of Cortex sophorae,
5 parts of rapeseed dregs of rice, 1 part of rock phosphate powder, 1 part of plant ash, 1 part of beans stalk crumb, vitamin B20.3 part, 4 parts of potassium superphosphate, lanthana
0.4 part, 0.5 part of cerium oxide, 0.06 part of gamma-polyglutamic acid, 8 parts of tea grounds, 1 part of nutritional additive, 2 parts of precipitated calcium carbonate.
A kind of high-yield flammulina velutipes culture medium matter, is made of the component of following parts by weight:
40 parts of obsolete fungus stick, 25 parts of cornstarch, 11 parts of poultry-dung, 13 parts of rice husk, 10 parts of nano diatomite, Cortex sophorae 15
Part, 12 parts of rapeseed dregs of rice, 3 parts of rock phosphate powder, 2 parts of plant ash, 5 parts of beans stalk crumb, vitamin B20.6 part, 6 parts of potassium superphosphate, oxygen
Change 0.7 part of lanthanum, 0.8 part of cerium oxide, 0.08 part of gamma-polyglutamic acid, 14 parts of tea grounds, 5 parts of nutritional additive, 4 parts of precipitated calcium carbonate.
A kind of high-yield flammulina velutipes culture medium matter, is made of the component of following parts by weight:
36 parts of obsolete fungus stick, 23 parts of cornstarch, 8 parts of poultry-dung, 10 parts of rice husk, 8 parts of nano diatomite, Cortex sophorae 12
Part, 9 parts of rapeseed dregs of rice, 2 parts of rock phosphate powder, 1.5 parts of plant ash, 3 parts of beans stalk crumb, vitamin B20.5 part, 5 parts of potassium superphosphate, oxygen
Change 0.6 part of lanthanum, 0.7 part of cerium oxide, 0.07 part of gamma-polyglutamic acid, 11 parts of tea grounds, 3 parts of nutritional additive, 3 parts of precipitated calcium carbonate.
The nutritional additive is prepared from the following raw materials in parts by weight: 15-24 parts of yam flour, 1-2 parts of citric acid chelated zinc, sulphur
Sour iron 0.5-1 parts, 10-16 parts of conch meal, 5-9 parts of pod Opulus leaf stirrup cotyledon, 7-9 parts of kudzu-vine root powder, 3-6 parts of Folium Artemisiae Argyi powder, green lemon powder
4-6 parts, 1-2 parts of astragalus membranaceus powder, 8-13 parts of sugar residue, the preparation method of the nutritional additive is to mix each raw material, crushes and stirs
It mixes uniformly to obtain the final product.
The nutritional additive is prepared from the following raw materials in parts by weight: 20 parts of yam flour, 1.5 parts of citric acid chelated zinc, sulfuric acid
0.8 part of iron, 13 parts of conch meal, 7 parts of pod Opulus leaf stirrup cotyledon, 8 parts of kudzu-vine root powder, 5 parts of Folium Artemisiae Argyi powder, green 5 parts of lemon powder, astragalus membranaceus powder 1.5
Part, 10 parts of sugar residue, the preparation method of the nutritional additive is to mix each raw material, crushes and stirs evenly to obtain the final product.
The average molecular weight of the gamma-polyglutamic acid is 5-200KDa.
Using the golden mushroom plantation method of above-mentioned high-yield flammulina velutipes culture medium matter, include the following steps:
(1) select strain: selection has just covered with no aging without miscellaneous bacteria, mycelia growth key-like, mycelia, has been suitble to local weather growth
Varieties in Flammulina velutipes as provenance；
(2) preparation of culture substrate: being exposed to the sun two days after obsolete fungus stick is crushed with poultry-dung together, then by obsolete fungus stick,
Poultry-dung, cornstarch, Cortex sophorae, nano diatomite, rapeseed dregs of rice, rock phosphate powder, plant ash, beans stalk crumb, tea grounds are added high
In fast blender, suitable quantity of water is added, is stirred 50-60 minutes, half of heap fermentation in the case where speed of agitator is 100-200r/min
Month, centre was with 12 hours each frequency mechanical stirrings, by vitamin B2It is added in high-speed mixer, is 150- in speed of agitator
250r/min is stirred 20-30 minutes, and rice husk, potassium superphosphate, lanthana, cerium oxide, gamma-polyglutamic acid, nutrition addition is being added
Agent, precipitated calcium carbonate stir 20-30 minutes in the case where speed of agitator is 200-300r/min, obtain mixture, under gnotobasis
Pack obtains culture substrate；
(3) it sterilizes: being carried out disinfection sterilizing using ultraviolet light irradiation to Bag Material；
(4) it is inoculated with: accessing Needle mushroom strain toward Bag Material in an aseptic environment, every bottle of strain connects 50-80 bags, ties after having connect
Sack carries out bacterium germination culture；
(5) Bag Material after inoculation: being moved on to the culturing room that can build half-light environment by cultivation, and cultural hypha process keeps environment
In half-light state, vegetative stage carries out half-light culture, and to be conducive to nutrition concentration, fruiting neat, and control culture room temperature is
20 DEG C -21 DEG C, when culture was to 30-33 days, cool down to culturing room, every 2 days temperature decline 1 DEG C, are down to when cultivating room temperature
At 13-15 DEG C, needle mushroom bacterium bag grows nascent former base, continue to be cooled to nascent former base it is long to 4.8-5.2cm when cut at bag
Reason；Culture room temperature is dropped to 6-8 DEG C, needle mushroom bacterium bag carries out low temperature inhibition, stronger ventilation amount, 2-3 to cutting bag treated
Former base of coming into being after it lodging is wilted, and inhibits to complete；Culture room temperature is promoted to 12-13 DEG C, continues to cultivate needle mushroom bacterium bag, make
The nascent former base continued growth wilted goes out new former base, and former base appearance is regenerated after 3-5 days, complete when continued growth is to 4.8-5.2cm
At regeneration, bagging is carried out at this time and continues to grow；Mushroom house temperature is down to 6-8 DEG C again, makes to regenerate former base continued growth, directly
To stem it is long to 13-15cm when be mature on the whole；
(6) it harvests: being harvested in time after needle mushroom is mature, not so stem is easy discoloration, rots；Ripeness standard be cap
Start to extend, if volume is sufficiently mature when saying and reduce quality on cap edge, every bag of needle mushroom size is adopted simultaneously when picking, use
Hand pinches stem base portion, and gently waving can adopt down, and then the compost that stem base portion is stained with is cut off again, is fitly put
It is sold in large bamboo or wicker basket.
Compared with prior art, the beneficial effects of the present invention are:
High-yield flammulina velutipes culture medium matter of the invention, raw material are also added with nutritional additive by rational proportion, for gold
The growth of needle mushroom provides comprehensive nutrition, while also improving the disease-resistant and anti-miscellaneous bacteria infection ability of needle mushroom, using of the invention
Needle mushroom quality and yield that culture substrate is planted are high, and mycelial growth rate is high, and the purseful period is short, success ratio of inoculation and
Yield rate is high.
Obsolete fungus stick provides nutriment abundant and suitable growing environment, poultry for Strains of Flammulina velutipes in the present invention
Excrement, Cortex sophorae, rapeseed dregs of rice, tea grounds, rice husk provide necessary nutrient for Strains of Flammulina velutipes, and nano diatomite can be with
Inhibit the growth of certain miscellaneous bacterias, rapeseed dregs of rice is used to adjust the carbon-nitrogen ratio of compost, and potassium superphosphate and precipitated calcium carbonate can make up
Phosphorus element, potassium, the deficiency of calcium element in compost, conducive to the fermentation maturity of compost.Gypsum can directly supplement sulphur in compost,
Hypocalcia, fixed gaseous nitrogen, accelerates compost to decompose, improves the cultivation yield of needle mushroom.
It is also added into cornstarch simultaneously, the viscosity that cornstarch is capable of increasing liquid makes liquid become gel, so
After culture substrate becomes gel, all raw material and water are all generally evenly distributed in culture medium, are greatly reduced and are precipitated
Situation.The amount that cornstarch is added in this programme simultaneously is few, and culture medium will not lead to its viscosity because Maize Starch Content is high
It is too big, needle mushroom can be unfavorable for instead and absorb nutrient.
The agricultural wastes such as obsolete fungus stick, tea grounds, rice husk, beans stalk crumb and poultry-dung are used for golden mushroom plantation by the present invention,
The agricultural wastes wasting of resources is not only reduced, but also reasonable nutrition arrangement promotes Flammulina velutipes mycelium growth, improves needle mushroom
Yield.
Lanthana and cerium oxide are added in culture substrate, introduces rare earth element, by lanthanum ion and cerium ion to calcium ion,
The physiological function of the displacement of the metal ions such as magnesium ion, zinc ion and some biological enzyme of competitive influence or large biological molecule.Therefore,
The needle mushroom cultivated by the culture substrate, can promote Growth of Flammulina Velutipes, vigor of supplying mother seed, and improve original seed quality.Add γ-
Polyglutamic acid, gamma-polyglutamic acid and rare earth element play synergistic effect, and slow release nutrient prevents nutrition stream
It loses, keeps absorption of the mycelia to nutrition.Further increase the quality and survival rate of needle mushroom.
Golden mushroom plantation method simple process proposed by the invention is easy to implement.
Specific embodiment
Embodiment 1
A kind of high-yield flammulina velutipes culture medium matter, is made of the component of following parts by weight:
30 parts of obsolete fungus stick, 20 parts of cornstarch, 6 parts of poultry-dung, 7 parts of rice husk, 6 parts of nano diatomite, 8 parts of Cortex sophorae,
5 parts of rapeseed dregs of rice, 1 part of rock phosphate powder, 1 part of plant ash, 1 part of beans stalk crumb, vitamin B20.3 part, 4 parts of potassium superphosphate, lanthana
0.4 part, 0.5 part of cerium oxide, 0.06 part of gamma-polyglutamic acid, 8 parts of tea grounds, 1 part of nutritional additive, 2 parts of precipitated calcium carbonate.
The nutritional additive is prepared from the following raw materials in parts by weight: 20 parts of yam flour, 1.5 parts of citric acid chelated zinc, sulfuric acid
0.8 part of iron, 13 parts of conch meal, 7 parts of pod Opulus leaf stirrup cotyledon, 8 parts of kudzu-vine root powder, 5 parts of Folium Artemisiae Argyi powder, green 5 parts of lemon powder, astragalus membranaceus powder 1.5
Part, 10 parts of sugar residue, the preparation method of the nutritional additive is to mix each raw material, crushes and stirs evenly to obtain the final product.
The average molecular weight of the gamma-polyglutamic acid is 5-200KDa.
Using the golden mushroom plantation method of above-mentioned high-yield flammulina velutipes culture medium matter, include the following steps:
(1) select strain: selection has just covered with no aging without miscellaneous bacteria, mycelia growth key-like, mycelia, has been suitble to local weather growth
Varieties in Flammulina velutipes as provenance；
(2) preparation of culture substrate: being exposed to the sun two days after obsolete fungus stick is crushed with poultry-dung together, then by obsolete fungus stick,
Poultry-dung, cornstarch, Cortex sophorae, nano diatomite, rapeseed dregs of rice, rock phosphate powder, plant ash, beans stalk crumb, tea grounds are added high
In fast blender, suitable quantity of water is added, is stirred 50-60 minutes, half of heap fermentation in the case where speed of agitator is 100-200r/min
Month, centre was with 12 hours each frequency mechanical stirrings, by vitamin B2It is added in high-speed mixer, is 150- in speed of agitator
250r/min is stirred 20-30 minutes, and rice husk, potassium superphosphate, lanthana, cerium oxide, gamma-polyglutamic acid, nutrition addition is being added
Agent, precipitated calcium carbonate stir 20-30 minutes in the case where speed of agitator is 200-300r/min, obtain mixture, under gnotobasis
Pack obtains culture substrate；
(3) it sterilizes: being carried out disinfection sterilizing using ultraviolet light irradiation to Bag Material；
(4) it is inoculated with: accessing Needle mushroom strain toward Bag Material in an aseptic environment, every bottle of strain connects 50-80 bags, ties after having connect
Sack carries out bacterium germination culture；
(5) Bag Material after inoculation: being moved on to the culturing room that can build half-light environment by cultivation, and cultural hypha process keeps environment
In half-light state, vegetative stage carries out half-light culture, and to be conducive to nutrition concentration, fruiting neat, and control culture room temperature is
20 DEG C -21 DEG C, when culture was to 30-33 days, cool down to culturing room, every 2 days temperature decline 1 DEG C, are down to when cultivating room temperature
At 13-15 DEG C, needle mushroom bacterium bag grows nascent former base, continue to be cooled to nascent former base it is long to 4.8-5.2cm when cut at bag
Reason；Culture room temperature is dropped to 6-8 DEG C, needle mushroom bacterium bag carries out low temperature inhibition, stronger ventilation amount, 2-3 to cutting bag treated
Former base of coming into being after it lodging is wilted, and inhibits to complete；Culture room temperature is promoted to 12-13 DEG C, continues to cultivate needle mushroom bacterium bag, make
The nascent former base continued growth wilted goes out new former base, and former base appearance is regenerated after 3-5 days, complete when continued growth is to 4.8-5.2cm
At regeneration, bagging is carried out at this time and continues to grow；Mushroom house temperature is down to 6-8 DEG C again, makes to regenerate former base continued growth, directly
To stem it is long to 13-15cm when be mature on the whole；
(6) it harvests: being harvested in time after needle mushroom is mature, not so stem is easy discoloration, rots；Ripeness standard be cap
Start to extend, if volume is sufficiently mature when saying and reduce quality on cap edge, every bag of needle mushroom size is adopted simultaneously when picking, use
Hand pinches stem base portion, and gently waving can adopt down, and then the compost that stem base portion is stained with is cut off again, is fitly put
It is sold in large bamboo or wicker basket.
Embodiment 2
A kind of high-yield flammulina velutipes culture medium matter, is made of the component of following parts by weight:
40 parts of obsolete fungus stick, 25 parts of cornstarch, 11 parts of poultry-dung, 13 parts of rice husk, 10 parts of nano diatomite, Cortex sophorae 15
Part, 12 parts of rapeseed dregs of rice, 3 parts of rock phosphate powder, 2 parts of plant ash, 5 parts of beans stalk crumb, vitamin B20.6 part, 6 parts of potassium superphosphate, oxygen
Change 0.7 part of lanthanum, 0.8 part of cerium oxide, 0.08 part of gamma-polyglutamic acid, 14 parts of tea grounds, 5 parts of nutritional additive, 4 parts of precipitated calcium carbonate.
The nutritional additive is prepared from the following raw materials in parts by weight: 20 parts of yam flour, 1.5 parts of citric acid chelated zinc, sulfuric acid
0.8 part of iron, 13 parts of conch meal, 7 parts of pod Opulus leaf stirrup cotyledon, 8 parts of kudzu-vine root powder, 5 parts of Folium Artemisiae Argyi powder, green 5 parts of lemon powder, astragalus membranaceus powder 1.5
Part, 10 parts of sugar residue, the preparation method of the nutritional additive is to mix each raw material, crushes and stirs evenly to obtain the final product.
The average molecular weight of the gamma-polyglutamic acid is 5-200KDa.
Using the golden mushroom plantation method of above-mentioned high-yield flammulina velutipes culture medium matter, include the following steps:
(1) select strain: selection has just covered with no aging without miscellaneous bacteria, mycelia growth key-like, mycelia, has been suitble to local weather growth
Varieties in Flammulina velutipes as provenance；
(2) preparation of culture substrate: being exposed to the sun two days after obsolete fungus stick is crushed with poultry-dung together, then by obsolete fungus stick,
Poultry-dung, cornstarch, Cortex sophorae, nano diatomite, rapeseed dregs of rice, rock phosphate powder, plant ash, beans stalk crumb, tea grounds are added high
In fast blender, suitable quantity of water is added, is stirred 50-60 minutes, half of heap fermentation in the case where speed of agitator is 100-200r/min
Month, centre was with 12 hours each frequency mechanical stirrings, by vitamin B2It is added in high-speed mixer, is 150- in speed of agitator
250r/min is stirred 20-30 minutes, and rice husk, potassium superphosphate, lanthana, cerium oxide, gamma-polyglutamic acid, nutrition addition is being added
Agent, precipitated calcium carbonate stir 20-30 minutes in the case where speed of agitator is 200-300r/min, obtain mixture, under gnotobasis
Pack obtains culture substrate；
(3) it sterilizes: being carried out disinfection sterilizing using ultraviolet light irradiation to Bag Material；
(4) it is inoculated with: accessing Needle mushroom strain toward Bag Material in an aseptic environment, every bottle of strain connects 50-80 bags, ties after having connect
Sack carries out bacterium germination culture；
(5) Bag Material after inoculation: being moved on to the culturing room that can build half-light environment by cultivation, and cultural hypha process keeps environment
In half-light state, vegetative stage carries out half-light culture, and to be conducive to nutrition concentration, fruiting neat, and control culture room temperature is
20 DEG C -21 DEG C, when culture was to 30-33 days, cool down to culturing room, every 2 days temperature decline 1 DEG C, are down to when cultivating room temperature
At 13-15 DEG C, needle mushroom bacterium bag grows nascent former base, continue to be cooled to nascent former base it is long to 4.8-5.2cm when cut at bag
Reason；Culture room temperature is dropped to 6-8 DEG C, needle mushroom bacterium bag carries out low temperature inhibition, stronger ventilation amount, 2-3 to cutting bag treated
Former base of coming into being after it lodging is wilted, and inhibits to complete；Culture room temperature is promoted to 12-13 DEG C, continues to cultivate needle mushroom bacterium bag, make
The nascent former base continued growth wilted goes out new former base, and former base appearance is regenerated after 3-5 days, complete when continued growth is to 4.8-5.2cm
At regeneration, bagging is carried out at this time and continues to grow；Mushroom house temperature is down to 6-8 DEG C again, makes to regenerate former base continued growth, directly
To stem it is long to 13-15cm when be mature on the whole；
(6) it harvests: being harvested in time after needle mushroom is mature, not so stem is easy discoloration, rots；Ripeness standard be cap
Start to extend, if volume is sufficiently mature when saying and reduce quality on cap edge, every bag of needle mushroom size is adopted simultaneously when picking, use
Hand pinches stem base portion, and gently waving can adopt down, and then the compost that stem base portion is stained with is cut off again, is fitly put
It is sold in large bamboo or wicker basket.
Embodiment 3
A kind of high-yield flammulina velutipes culture medium matter, is made of the component of following parts by weight:
36 parts of obsolete fungus stick, 23 parts of cornstarch, 8 parts of poultry-dung, 10 parts of rice husk, 8 parts of nano diatomite, Cortex sophorae 12
Part, 9 parts of rapeseed dregs of rice, 2 parts of rock phosphate powder, 1.5 parts of plant ash, 3 parts of beans stalk crumb, vitamin B20.5 part, 5 parts of potassium superphosphate, oxygen
Change 0.6 part of lanthanum, 0.7 part of cerium oxide, 0.07 part of gamma-polyglutamic acid, 11 parts of tea grounds, 3 parts of nutritional additive, 3 parts of precipitated calcium carbonate.
The nutritional additive is prepared from the following raw materials in parts by weight: 20 parts of yam flour, 1.5 parts of citric acid chelated zinc, sulfuric acid
0.8 part of iron, 13 parts of conch meal, 7 parts of pod Opulus leaf stirrup cotyledon, 8 parts of kudzu-vine root powder, 5 parts of Folium Artemisiae Argyi powder, green 5 parts of lemon powder, astragalus membranaceus powder 1.5
Part, 10 parts of sugar residue, the preparation method of the nutritional additive is to mix each raw material, crushes and stirs evenly to obtain the final product.
The average molecular weight of the gamma-polyglutamic acid is 5-200KDa.
Using the golden mushroom plantation method of above-mentioned high-yield flammulina velutipes culture medium matter, include the following steps:
(1) select strain: selection has just covered with no aging without miscellaneous bacteria, mycelia growth key-like, mycelia, has been suitble to local weather growth
Varieties in Flammulina velutipes as provenance；
(2) preparation of culture substrate: being exposed to the sun two days after obsolete fungus stick is crushed with poultry-dung together, then by obsolete fungus stick,
Poultry-dung, cornstarch, Cortex sophorae, nano diatomite, rapeseed dregs of rice, rock phosphate powder, plant ash, beans stalk crumb, tea grounds are added high
In fast blender, suitable quantity of water is added, is stirred 50-60 minutes, half of heap fermentation in the case where speed of agitator is 100-200r/min
Month, centre was with 12 hours each frequency mechanical stirrings, by vitamin B2It is added in high-speed mixer, is 150- in speed of agitator
250r/min is stirred 20-30 minutes, and rice husk, potassium superphosphate, lanthana, cerium oxide, gamma-polyglutamic acid, nutrition addition is being added
Agent, precipitated calcium carbonate stir 20-30 minutes in the case where speed of agitator is 200-300r/min, obtain mixture, under gnotobasis
Pack obtains culture substrate；
(3) it sterilizes: being carried out disinfection sterilizing using ultraviolet light irradiation to Bag Material；
(4) it is inoculated with: accessing Needle mushroom strain toward Bag Material in an aseptic environment, every bottle of strain connects 50-80 bags, ties after having connect
Sack carries out bacterium germination culture；
(5) Bag Material after inoculation: being moved on to the culturing room that can build half-light environment by cultivation, and cultural hypha process keeps environment
In half-light state, vegetative stage carries out half-light culture, and to be conducive to nutrition concentration, fruiting neat, and control culture room temperature is
20 DEG C -21 DEG C, when culture was to 30-33 days, cool down to culturing room, every 2 days temperature decline 1 DEG C, are down to when cultivating room temperature
At 13-15 DEG C, needle mushroom bacterium bag grows nascent former base, continue to be cooled to nascent former base it is long to 4.8-5.2cm when cut at bag
Reason；Culture room temperature is dropped to 6-8 DEG C, needle mushroom bacterium bag carries out low temperature inhibition, stronger ventilation amount, 2-3 to cutting bag treated
Former base of coming into being after it lodging is wilted, and inhibits to complete；Culture room temperature is promoted to 12-13 DEG C, continues to cultivate needle mushroom bacterium bag, make
The nascent former base continued growth wilted goes out new former base, and former base appearance is regenerated after 3-5 days, complete when continued growth is to 4.8-5.2cm
At regeneration, bagging is carried out at this time and continues to grow；Mushroom house temperature is down to 6-8 DEG C again, makes to regenerate former base continued growth, directly
To stem it is long to 13-15cm when be mature on the whole；
(6) it harvests: being harvested in time after needle mushroom is mature, not so stem is easy discoloration, rots；Ripeness standard be cap
Start to extend, if volume is sufficiently mature when saying and reduce quality on cap edge, every bag of needle mushroom size is adopted simultaneously when picking, use
Hand pinches stem base portion, and gently waving can adopt down, and then the compost that stem base portion is stained with is cut off again, is fitly put
It is sold in large bamboo or wicker basket.
Claims (8)
1. a kind of high-yield flammulina velutipes culture medium matter, which is characterized in that be made of the component of following parts by weight:
30-40 parts of obsolete fungus stick, 20-25 parts of cornstarch, 6-11 parts of poultry-dung, 7-13 parts of rice husk, 6-10 parts of nano diatomite,
8-15 parts of Cortex sophorae, 5-12 parts of rapeseed dregs of rice, 1-3 parts of rock phosphate powder, 1-2 parts of plant ash, 1-5 parts of beans stalk crumb, vitamin B2
0.3-0.6 parts, 4-6 parts of potassium superphosphate, 0.4-0.7 parts of lanthana, 0.5-0.8 parts of cerium oxide, gamma-polyglutamic acid 0.06-0.08
Part, 8-14 parts of tea grounds, 1-5 parts of nutritional additive, 2-4 parts of precipitated calcium carbonate.
2. high-yield flammulina velutipes culture medium matter according to claim 1, which is characterized in that by the component system of following parts by weight
At:
30 parts of obsolete fungus stick, 20 parts of cornstarch, 6 parts of poultry-dung, 7 parts of rice husk, 6 parts of nano diatomite, 8 parts of Cortex sophorae, rape
5 parts of the seed dregs of rice, 1 part of rock phosphate powder, 1 part of plant ash, 1 part of beans stalk crumb, vitamin B20.3 part, 4 parts of potassium superphosphate, lanthana 0.4
Part, 0.5 part of cerium oxide, 0.06 part of gamma-polyglutamic acid, 8 parts of tea grounds, 1 part of nutritional additive, 2 parts of precipitated calcium carbonate.
3. high-yield flammulina velutipes culture medium matter according to claim 1, which is characterized in that by the component system of following parts by weight
At:
40 parts of obsolete fungus stick, 25 parts of cornstarch, 11 parts of poultry-dung, 13 parts of rice husk, 10 parts of nano diatomite, 15 parts of Cortex sophorae,
12 parts of rapeseed dregs of rice, 3 parts of rock phosphate powder, 2 parts of plant ash, 5 parts of beans stalk crumb, vitamin B20.6 part, 6 parts of potassium superphosphate, oxidation
0.7 part of lanthanum, 0.8 part of cerium oxide, 0.08 part of gamma-polyglutamic acid, 14 parts of tea grounds, 5 parts of nutritional additive, 4 parts of precipitated calcium carbonate.
4. high-yield flammulina velutipes culture medium matter according to claim 1, which is characterized in that by the component system of following parts by weight
At:
36 parts of obsolete fungus stick, 23 parts of cornstarch, 8 parts of poultry-dung, 10 parts of rice husk, 8 parts of nano diatomite, 12 parts of Cortex sophorae, oil
9 parts of rapeseed dregs, 2 parts of rock phosphate powder, 1.5 parts of plant ash, 3 parts of beans stalk crumb, vitamin B20.5 part, 5 parts of potassium superphosphate, lanthana
0.6 part, 0.7 part of cerium oxide, 0.07 part of gamma-polyglutamic acid, 11 parts of tea grounds, 3 parts of nutritional additive, 3 parts of precipitated calcium carbonate.
5. high-yield flammulina velutipes culture medium matter according to claim 1, which is characterized in that the gamma-polyglutamic acid is averaged
Molecular weight is 5-200KDa.
6. high-yield flammulina velutipes culture medium matter according to claim 1, which is characterized in that the nutritional additive is by following heavy
Amount part raw material is made: 10-16 parts of 15-24 parts of yam flour, 1-2 parts of citric acid chelated zinc, 0.5-1 parts of ferric sulfate, conch meal, Jia Opulus
5-9 parts of leaf stirrup cotyledon, 7-9 parts of kudzu-vine root powder, 3-6 parts of Folium Artemisiae Argyi powder, green 4-6 parts of lemon powder, 1-2 parts of astragalus membranaceus powder, 8-13 parts of sugar residue, institute
The preparation method for stating nutritional additive is to mix each raw material, crushes and stirs evenly to obtain the final product.
7. high-yield flammulina velutipes culture medium matter according to claim 6, which is characterized in that the nutritional additive is by following heavy
Amount part raw material is made: 13 parts of 20 parts of yam flour, 1.5 parts of citric acid chelated zinc, 0.8 part of ferric sulfate, conch meal, Jia Opulus leaf raspberries
7 parts of leaf, 8 parts of kudzu-vine root powder, 5 parts of Folium Artemisiae Argyi powder, green 5 parts of lemon powder, 1.5 parts of astragalus membranaceus powder, 10 parts of sugar residue.
8. using the golden mushroom plantation method of any one of the claim 1-7 high-yield flammulina velutipes culture medium matter, which is characterized in that
Include the following steps:
(1) it selects strain: selecting the gold for just having covered with no aging without miscellaneous bacteria, mycelia growth key-like, mycelia, being suitble to local weather growth
Needle mushroom kind is as provenance；
(2) preparation of culture substrate: being exposed to the sun two days after obsolete fungus stick is crushed with poultry-dung together, then by obsolete fungus stick, poultry
High-speed stirring is added in excrement, cornstarch, Cortex sophorae, nano diatomite, rapeseed dregs of rice, rock phosphate powder, plant ash, beans stalk crumb, tea grounds
It mixes in machine, adds suitable quantity of water, stirred 50-60 minutes in the case where speed of agitator is 100-200r/min, heap fermentation two weeks, in
Between with 12 hours each frequency mechanical stirrings, by vitamin B2It is added in high-speed mixer, is 150-250r/ in speed of agitator
Min stir 20-30 minute, be added rice husk, potassium superphosphate, lanthana, cerium oxide, gamma-polyglutamic acid, nutritional additive, gently
Matter calcium carbonate stirs 20-30 minutes in the case where speed of agitator is 200-300r/min, obtains mixture, pack under gnotobasis
To culture substrate；
(3) it sterilizes: being carried out disinfection sterilizing using ultraviolet light irradiation to Bag Material；
(4) it is inoculated with: accessing Needle mushroom strain toward Bag Material in an aseptic environment, every bottle of strain connects 50-80 bags, ties sack after having connect
Carry out bacterium germination culture；
(5) Bag Material after inoculation: being moved on to the culturing room that can build half-light environment by cultivation, and cultural hypha process keeps environment to be in
Half-light state, vegetative stage carries out half-light culture, and to be conducive to nutrition concentration, fruiting neat, and control culture room temperature is 20
DEG C -21 DEG C, when culture was to 30-33 days, cool down to culturing room, every 2 days temperature decline 1 DEG C, are down to 13- when cultivating room temperature
At 15 DEG C, needle mushroom bacterium bag grows nascent former base, continue to be cooled to nascent former base it is long to 4.8-5.2cm when carry out cutting bag processing；
Culture room temperature is dropped to 6-8 DEG C, needle mushroom bacterium bag carries out low temperature inhibition, stronger ventilation amount, after 2-3 days to cutting bag treated
Nascent former base lodging is wilted, and inhibits to complete；Culture room temperature is promoted to 12-13 DEG C, continues to cultivate needle mushroom bacterium bag, makes to wilt
Nascent former base continued growth go out new former base, former base is regenerated after 3-5 days and is occurred, when continued growth is to 4.8-5.2cm, is completed again
It is raw, bagging is carried out at this time to be continued to grow；Mushroom house temperature is down to 6-8 DEG C again, makes to regenerate former base continued growth, until bacterium
Handle it is long to 13-15cm when be mature on the whole；
(6) it harvests: being harvested in time after needle mushroom is mature, not so stem is easy discoloration, rots；Ripeness standard is that cap has started
Extension, if on cap edge volume when saying sufficiently maturation and reduce quality, every bag of needle mushroom size is adopted simultaneously when picking, is pinched with hand
Firmly stem base portion, gently waving can adopt down, and then the compost that stem base portion is stained with is cut off again, is fitly placed on square-bottomed bamboo basket
It is sold in basket.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110506567A (en) * | 2019-08-22 | 2019-11-29 | 江苏东越生物技术发展股份有限公司 | A kind of mushroom industrialized acid-proof of acupuncture needle loses highly-breathable culture medium and preparation method thereof |
| CN110972813A (en) * | 2019-12-27 | 2020-04-10 | 安徽农业大学 | Agaricus bisporus culture medium and preparation method thereof |
| CN116686630A (en) * | 2023-07-04 | 2023-09-05 | 上海永大菌业有限公司 | Method for cultivating flammulina velutipes by using crop straws |
-
2018
- 2018-10-09 CN CN201811174317.1A patent/CN109168957A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110506567A (en) * | 2019-08-22 | 2019-11-29 | 江苏东越生物技术发展股份有限公司 | A kind of mushroom industrialized acid-proof of acupuncture needle loses highly-breathable culture medium and preparation method thereof |
| CN110972813A (en) * | 2019-12-27 | 2020-04-10 | 安徽农业大学 | Agaricus bisporus culture medium and preparation method thereof |
| CN116686630A (en) * | 2023-07-04 | 2023-09-05 | 上海永大菌业有限公司 | Method for cultivating flammulina velutipes by using crop straws |
| CN116686630B (en) * | 2023-07-04 | 2024-01-30 | 上海永大菌业有限公司 | Method for cultivating flammulina velutipes by using crop straws |
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