CN114981291B - Hmo产生 - Google Patents

Hmo产生 Download PDF

Info

Publication number
CN114981291B
CN114981291B CN202180010202.2A CN202180010202A CN114981291B CN 114981291 B CN114981291 B CN 114981291B CN 202180010202 A CN202180010202 A CN 202180010202A CN 114981291 B CN114981291 B CN 114981291B
Authority
CN
China
Prior art keywords
lacto
lnt
gene
hmo
genetically modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202180010202.2A
Other languages
English (en)
Chinese (zh)
Other versions
CN114981291A (zh
Inventor
M·帕帕扎基斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Glycom AS
Original Assignee
Glycom AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glycom AS filed Critical Glycom AS
Publication of CN114981291A publication Critical patent/CN114981291A/zh
Application granted granted Critical
Publication of CN114981291B publication Critical patent/CN114981291B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN202180010202.2A 2020-01-23 2021-01-22 Hmo产生 Active CN114981291B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA202000088 2020-01-23
DKPA202000088 2020-01-23
PCT/EP2021/051466 WO2021148610A1 (en) 2020-01-23 2021-01-22 Hmo production

Publications (2)

Publication Number Publication Date
CN114981291A CN114981291A (zh) 2022-08-30
CN114981291B true CN114981291B (zh) 2024-12-06

Family

ID=74505190

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180010202.2A Active CN114981291B (zh) 2020-01-23 2021-01-22 Hmo产生

Country Status (6)

Country Link
US (1) US20230109661A1 (https=)
EP (1) EP4093748A1 (https=)
JP (1) JP2023511524A (https=)
CN (1) CN114981291B (https=)
BR (1) BR112022014420A2 (https=)
WO (1) WO2021148610A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK181497B1 (en) 2021-05-17 2024-03-12 Dsm Ip Assets Bv ENHANCING FORMATION OF THE HMOS LNT AND/OR LNnT BY MODIFYING LACTOSE IMPORT IN THE CELL
DK181991B1 (en) 2021-11-11 2025-05-15 Dsm Ip Assets Bv Combined fermentation process for producing one or more human milk oligosaccharide(s) (hmo(s)) in escherichia coli
US20260028634A1 (en) 2022-03-02 2026-01-29 Dsm Ip Assets B.V. New sialyltransferases for in vivo synthesis of 3'sl and 6'sl
DK181319B1 (en) 2022-03-02 2023-08-10 Dsm Ip Assets Bv Genetically engineered cells and methods comprising use of a sialyltransferase for in vivo synthesis of 3’sl
DK181683B1 (en) 2022-03-02 2024-09-20 Dsm Ip Assets Bv Cells exprssing new sialyltransferases for in vivo synthesis of lst-a, methods using same and constructs encoding said sialyltransferases
US20250290078A1 (en) 2022-04-29 2025-09-18 Dsm Ip Assets B.V. Hmo producing microorganism with increased robustness towards glucose gradients
EP4577061A1 (en) 2022-08-25 2025-07-02 DSM IP Assets B.V. Hybrid method for producing complex hmos
WO2024110667A1 (en) 2022-11-25 2024-05-30 Dsm Ip Assets B.V. Two-strain system for producing oligosaccharides
CN120677242A (zh) 2023-02-24 2025-09-19 帝斯曼知识产权资产管理有限公司 用于体内合成人乳寡糖的产物特异性转运蛋白
DK181822B1 (en) 2023-06-07 2025-01-30 Dsm Ip Assets Bv Genetically engineered strains with reduced byproduct formation and methods and uses of same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015150328A1 (en) * 2014-03-31 2015-10-08 Jennewein Biotechnologie Gmbh Total fermentation of oligosaccharides

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2440661T3 (en) * 2009-06-08 2018-03-12 Jennewein Biotechnologie Gmbh HMO synthesis
EP2675899A4 (en) 2011-02-16 2014-09-10 Glycosyn LLC BIOSYNTHESIS OF HUMAN MILK OLIGOSACCHARIDES IN MANIPULATED BACTERIA
DE202015009782U1 (de) 2014-06-11 2020-02-10 Glycom A/S Trennung von 2'-O-Fucosyllactose aus Fermentationsbrühe
EP3191499A4 (en) 2014-09-09 2018-06-06 Glycosyn LLC Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides
US10676770B2 (en) 2014-12-16 2020-06-09 Glycom A/S Separation of 2′-FL from a fermentation broth
EP3141610A1 (en) 2015-09-12 2017-03-15 Jennewein Biotechnologie GmbH Production of human milk oligosaccharides in microbial hosts with engineered import / export
DE202017007249U1 (de) 2016-03-07 2020-04-23 Glycom A/S Abtrennung von Oligosacchariden aus der Fermentationsbrühe
EP4674526A3 (en) 2016-04-19 2026-04-01 Glycom A/S Separation of oligosaccharides from fermentation broth
ES2856749T3 (es) 2016-10-29 2021-09-28 Chr Hansen Hmo Gmbh Proceso para la producción de oligosacáridos fucosilados
US11608504B2 (en) * 2017-12-21 2023-03-21 Glycom A/S Nucleic acid construct for in vitro and in vivo gene expression
US20190323053A1 (en) 2018-04-23 2019-10-24 Dupont Nutrition Biosciences Aps Increasing activity of 2? fucosyllactose transporters endogenous to microbial cells
US20190323052A1 (en) 2018-04-23 2019-10-24 Dupont Nutrition Biosciences Aps Increasing export of 2? fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
EP3569713A1 (en) * 2018-05-16 2019-11-20 Jennewein Biotechnologie GmbH Use of glycosidases in the production of oligosaccharides
US10519475B1 (en) 2018-11-21 2019-12-31 Amyris, Inc. Biosynthesis of compounds in yeast

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015150328A1 (en) * 2014-03-31 2015-10-08 Jennewein Biotechnologie Gmbh Total fermentation of oligosaccharides

Also Published As

Publication number Publication date
JP2023511524A (ja) 2023-03-20
US20230109661A1 (en) 2023-04-06
BR112022014420A2 (pt) 2022-09-27
WO2021148610A1 (en) 2021-07-29
CN114981291A (zh) 2022-08-30
WO2021148610A9 (en) 2022-04-28
EP4093748A1 (en) 2022-11-30

Similar Documents

Publication Publication Date Title
CN114981290B (zh) Hmo生产
CN115003687B (zh) Hmo生产
US12351806B2 (en) HMO production
CN114981291B (zh) Hmo产生
US20240102063A1 (en) New major facilitator superfamily (mfs) protein (fred) in production of sialylated hmos
US20240043891A1 (en) A dfl-producing strain
US20230109937A1 (en) New major facilitator superfamily (mfs) protein (fred) in hmo production
EA046260B1 (ru) Получение огм
CN116802302A (zh) 唾液酸化hmo生产中的新主要易化子超家族（mfs）蛋白（fred）
EA046241B1 (ru) Получение огм
EA046005B1 (ru) НОВЫЙ БЕЛОК СУПЕРСЕМЕЙСТВА ОСНОВНЫХ ПЕРЕНОСЧИКОВ (MFS) (Fred) В ПРОИЗВОДСТВЕ ОГМ
EA046248B1 (ru) Получение огм

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant