CN114948940A

CN114948940A - Natural product monomer composition with hair-blacking and hair-care functions and application thereof

Info

Publication number: CN114948940A
Application number: CN202210676346.8A
Authority: CN
Inventors: 刘小溪; 季辉; 乐艳; 陈露露
Original assignee: Haoxi Hainan Biotechnology Co ltd
Current assignee: Haoxi Hainan Biotechnology Co ltd
Priority date: 2022-06-15
Filing date: 2022-06-15
Publication date: 2022-08-30
Anticipated expiration: 2042-06-15
Also published as: CN114948940B

Abstract

The invention belongs to the field of medical cosmetics, and particularly relates to a natural product monomer composition with a hair blacking and hair care function and application thereof. The natural product monomer composition comprises pinocembrin and quercetin, wherein the proportion of the pinocembrin and the quercetin in the composition in parts by weight is 10: 1-1: 10. studies have shown that the composition significantly enhances tyrosinase activity, as well as promotes telogen-phase hair follicles into the regenerative cycle, promoting hair growth. The composition has the effects of blackening and strengthening hair and the like, has the advantages of simple formula, exact effective components, safety in use, no adverse reaction and the like, and has wide market prospect.

Description

Natural product monomer composition with hair-blacking and hair-care functions and application thereof

Technical Field

The invention belongs to the field of medical cosmetics, and particularly relates to a natural product monomer composition with a hair blacking and hair care function and application thereof.

Background

According to the traditional Chinese medicine, the hair is considered to be surplus in blood, the kidney stores essence, the essence is bloomed in the hair, the hair growth depends on the nourishment of the essence and the blood, the dense, dark and bright hair represents health, and a good image can be given to people. However, in recent years, there have been various problems of hair such as endocrine imbalance, exuberant secretion of hair oil and fat, alopecia, and canities in modern people due to environmental pollution and increased pressure for life. Alopecia, premature gray hair, white hair, etc. can cause bad psychology such as self-inferior and anxiety, and serious people even affect social activities, so modern young people generally pay more attention to hair care.

White hair is generally classified into premature gray hair, commonly called "pale hair", and senile white hair. Premature gray hair often occurs in young adults or children, and most of the premature gray hair belongs to autosomal dominant inheritance. Senile canities are common, generally, from the age of 35, tyrosinase in human hair is gradually lost, and the tyrosinase is specifically expressed in that melanin generated by melanocytes in hair papillae is reduced, so that pigment in hair shafts disappears, canities begin to appear, the canities increase after the age of 60, the number of the melanocytes in gray hair is normal, cells can contain vacuoles, and melanosomes with normal appearance but few melanin are contained. The number of melanocytes is rare or lacking in the white hair, the melanin granules are obviously reduced, and the melanocytes and the melanin granules are rarely found in the papilla of the hair bulb. Although the senile white hair belongs to a normal physiological phenomenon, the age of the senile white hair is obviously advanced, the degree is increased, and the speed is accelerated in recent years, which is obviously not a normal state.

Research shows that tyrosinase is a key enzyme for melanin synthesis, and the reduction and loss of tyrosinase elution function in melanin cells of skin and hair follicles can cause the reduction or loss of skin melanin, so that corresponding diseases, such as withered and yellow hair, no color, easy breakage and the like, are caused. At present, methods for solving the problems of white hair, alopecia, little hair and the like mainly comprise diet conditioning, oral administration of traditional Chinese medicines and direct use of hair dyes. However, dietary conditioning, while safe, is not effective significantly. The long-term use of the traditional Chinese medicine for oral administration may have certain toxic and side effects. The hair dye can treat the symptoms and not the root causes, and more importantly, the hair dye can also cause allergy to the human body and influence on the environment.

With the discovery of social economy, products with natural green components are favored by more and more consumers, and the Chinese herbal medicine resource is rich in China and the traditional medicine is profound, so the method has great advantages for developing herbal plant hair-blacking and hair-care products. The theory of traditional Chinese medicine considers that the principle of external treatment is that of internal treatment, and the medicine of external treatment is that of internal treatment, so that the traditional Chinese medicine formula for oral administration can be externally used. The traditional Chinese medicine considers that the formation of white hair and alopecia is related to deficiency of liver and kidney and deficiency of blood and qi, so the traditional Chinese medicine mainly treats the white hair and the alopecia by nourishing blood, promoting blood circulation, tonifying liver and kidney. At present, researches find that Chinese herbal medicines for preventing and blackening hair and strengthening hair recorded in medical books mainly comprise Chinese herbal medicines for promoting scalp blood circulation, improving hair follicle growth and development, enhancing tyrosinase activity and increasing melanin content in hair follicles. The development of safe, stable and strong herbal hair-blacking and hair-care products by combining modern science with ancient medical research of China is favored by consumers.

The inventors of the present application have concentrated on studies on natural products and related monomers of extracts thereof in terms of hair-blacking and hair-care for many years, and have unexpectedly found that combining vaccinium bracteatum thunb leaf extract, cacumen biotae extract, eclipta alba extract and peppermint leaf oil in a specific ratio can produce a strong hair-blacking and hair-care effect during the research.

The invention is further improved on the basis, and the inventor screens out a natural product monomer composition with the function of blacking and strengthening the hair, which consists of the main active ingredient, namely the pinocembrin from the cacumen biotae extract and the main active ingredient, namely the quercetin from the vaccinium bracteatum leaf extract, by deeply researching and compounding the natural product monomer related to blacking and strengthening the hair in the natural product extract, wherein the two ingredients have obvious synergistic interaction, so that a natural product monomer composition product with the function of blacking and strengthening the hair, which has a simpler formula, more exact effective ingredients, safety and effectiveness, is developed.

Disclosure of Invention

In order to solve the technical problems, the invention provides a natural product monomer composition with the functions of blacking and strengthening the hair, which has the effects of blacking the hair, strengthening the hair and the like, also has the advantages of simple formula, exact effective components, safe use, no adverse reaction and the like, and has wide market prospect.

Specifically, the invention is realized by the following technical schemes:

in a first aspect, the present invention provides a natural product monomer composition having a hair blackening and hair health function, the natural product monomer composition comprising pinocembrin and quercetin, wherein the ratio of pinocembrin and quercetin in the composition is 10: 1-1: 10.

as an alternative, wherein the ratio of pinocembrin and quercetin in the composition is 5: 1-1: 5.

as an alternative, wherein the ratio of pinocembrin and quercetin in the composition is 5: 1. 4: 1. 3: 1. 2: 1. 1: 1. 1: 2. 1: 3. 1: 4 or 1: 5.

preferably, wherein the ratio of pinosynolactone to quercetin in the composition in parts by weight is 4: 1.

more preferably, the composition consists of pinocembrin and quercetin.

Alternatively, in the above composition, the composition significantly enhances tyrosinase activity, and promotes telogen-phase hair follicles to enter a regenerative cycle, promoting hair growth.

In a second aspect, the present invention provides the use of a composition according to the first aspect above for the preparation of a hair-blackening and hair-care product.

Alternatively, in the above use, the product is a product for external use.

Alternatively, in the above use, the product is a pharmaceutical or cosmetic.

Alternatively, in the above use, the external product is a gel, an ointment, a cream, a foam, a shampoo, a conditioner, or a spray-type liquid preparation.

It is to be understood that within the scope of the present invention, the above-described features of the present invention and those specifically described below (e.g., in the examples) may be combined with each other to form new or preferred embodiments. For reasons of space, they are not described in detail herein.

Compared with the prior art, the invention has the following beneficial effects:

the invention combines the Korean pine lactone and the quercetin according to a specific proportion, can generate strong hair-blacking and hair-care effects, and the two components have obvious synergistic interaction. The natural product monomer composition has the effects of blackening and strengthening hair and the like, has the advantages of simple formula, exact effective components, safety in use, no adverse reaction and the like, and has wide market prospect.

Detailed Description

The invention is further illustrated with reference to specific examples. It should be understood that the specific embodiments described herein are illustrative only and are not limiting upon the scope of the invention.

The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or instruments used are conventional products which are not indicated by manufacturers and are available from normal sources.

The experimental procedures in the following examples are all conventional ones unless otherwise specified. The test materials used in the following examples are all commercially available products unless otherwise specified.

A medicinal plant, WUWEIZHISHUZI (Vaccinium bracteatum Thunb.) is a plant of Vaccinium of Ericaceae, such as Vaccinium bracteatum, and Shuangshu. The leaves of Vaccinium bracteatum from the dictionary of Chinese medicine have the effects of invigorating vital essence, strengthening tendons and bones, improving eyesight and stopping diarrhea. Traditional Chinese medicine holds that blueberry medicine prepared by juicing tender leaves of blueberry has the effects of reducing weight, improving eyesight, blackening hair, retaining youthful looks and prolonging life. Oriental blueberry leaf is complete or has sawteeth, and tender leaf is an important medicinal material, and contains anthocyanin, friedelin, epifriedelin alcohol, beta-sitosterol, ursolic acid, blue melanin (the main component is quercetin), p-hydroxycinnamic acid, ursolic acid, oleanolic acid and the like. Folium Vaccinii Bracteati has pharmacological activities of promoting resynthesis of rhodophyll, improving blood microcirculation, resisting ulcer, resisting inflammation, and resisting aging. Chinese patent CN102000017A discloses a vaccinium bracteatum plant hair blackening cream, and provides a plant hair dyeing cream which takes natural plant vaccinium bracteatum leaf extract as a main dyeing agent, has good blackening effect, and has hair care and oxidation functions. Chinese patent CN111920717A discloses a natural hair dye and a preparation method and a use method thereof. The hair dye comprises two separate preparations, wherein one preparation comprises folium Vaccinii Bracteati extract, and the other preparation comprises ferric ion. The hair dye is suitable for dyeing white hair black, and the hair black is pure and has good and natural color.

The main source of blue melanin in vaccinium bracteatum leaves is Quercetin, Quercetin (Quercetin), molecular formula C ₁₅ H ₁₀ O ₇ (ii) a Molecular weight: 302.236, its chemical name is 3,3',4',5, 7-pentahydroxyflavone. Quercetin belongs to flavonol compounds, which have various biological activities and can resist free radicals, complex or capture free radicals to prevent lipid peroxidation of organisms; can directly inhibit tumor and effectively play the role of cancer prevention and resistance; has strong biological activity in the aspects of antibiosis, anti-inflammation, anti-allergy and prevention of diabetic complications. In addition, quercetin has the effects of lowering blood pressure, enhancing capillary resistance, reducing capillary fragility, reducing blood lipid, dilating coronary artery, increasing coronary blood flow, and can be used for adjuvant treatment of coronary heart disease and hypertension. Quercetin is nontoxic, and therefore, has great significance for treating and preventing cancer, aging and cardiovascular diseases, and has great development value. Chinese patent CN112353819A discloses a hair growth agent for promoting hair growth or repairing hair follicle tissue, whereinTo contain quercetin. Chinese patent CN108201513A discloses a hair composition and a hair tonic, wherein the hair tonic composition contains fullerene component and optionally quercetin.

"biota orientalis (Platycladi cacume)" refers to the dried and tender tips and leaves of biota orientalis. According to traditional Chinese medicine, the cacumen biotae is fragrant in smell, bitter and astringent in taste and cold in nature and enters lung, liver and spleen channels. Has the effects of cooling blood, stopping bleeding, eliminating phlegm, relieving cough, promoting hair growth and blackening hair. Can be used for treating hematemesis, hemoptysis, hematochezia, metrorrhagia, metrostaxis, cough due to lung heat, alopecia due to blood heat, and canities. Chinese patent CN112842972A discloses a hair-blackening liquid containing cacumen biotae extract, which can effectively activate tyrosinase activity and significantly increase melanin in new hair roots. It is reported in the literature that petroleum ether extract of cacumen biotae can promote hair growth, and the main active ingredients of the active site of the traditional Chinese medicine are isopimarenol and pinocembrin (for example, see Miao Hui, "chemical ingredient and pharmacological activity research of cacumen biotae," Master academic paper of Jilin agriculture university, 2019, 02).

The natural product monomer pinosylvin and quercetin used in the invention can be extracted and separated from oriental blueberry leaves, cacumen biotae and other plants containing the active ingredients by adopting a conventional extraction and purification method in the field of natural medicinal chemistry, and can also be purchased from commercially available products.

Preparation examples:

example 1:

a natural product monomer composition with a hair blacking and hair care function, the natural product monomer composition consisting of Korean pine lactone and quercetin, wherein the ratio of Korean pine lactone to quercetin in parts by weight in the composition is 4: 1.

example 2:

a natural product monomer composition with a hair blacking and hair care function, the natural product monomer composition consisting of Korean pine lactone and quercetin, wherein the proportion of the Korean pine lactone and the quercetin in the composition in parts by weight is 5: 1.

example 3:

a natural product monomer composition with a hair blacking and hair care function, the natural product monomer composition consisting of Korean pine lactone and quercetin, wherein the proportion of the Korean pine lactone and the quercetin in the composition in parts by weight is 3: 1.

comparative example 1:

a natural product monomer pinocembrin.

Comparative example 2:

a natural product monomer quercetin.

Comparative example 3:

a natural product monomer composition consisting of pinocembrin and quercetin, wherein the ratio of pinocembrin and naringenin in the composition, in parts by weight, is 4: 1.

in the following effect experiments, the test substance was prepared by adding a certain amount of the compositions described in examples 1 to 3 and comparative examples 1 to 3 to a suitable solvent or cosmetic base according to actual needs by those skilled in the art.

Effect example 1: tyrosinase activation experiment

Preparing an L-dopa solution with the concentration of 1g/L by using a 0.2mol/L phosphoric acid buffer solution (the pH value is 6.8); preparing a tyrosinase solution with the concentration of 100U/mL by adopting 0.2mol/L phosphoric acid buffer solution (the pH value is 6.8); preparing a liquid medicine to be tested containing the composition of examples 1-3 or comparative examples 1-3 by using 0.2mol/L phosphoric acid buffer solution (pH is 6.8) (the concentration of each component in the composition is shown in Table 1); adding 40 mu L of L-dopa solution, 40 mu L of liquid medicine to be detected and/or 40 mu L of tyrosinase solution into a 96-well plate, and then reacting in a water bath at 30 ℃ for 40 min; testing the absorbance at 490nm after the reaction is finished, repeating each group of experiments for 3 times, and taking the average value of the 3 tests of the absorbance of each group of experiments to obtain the absorbance C; according to the method, a phosphate buffer solution is used for replacing a tyrosinase solution, and the absorbance D is obtained through testing; replacing the liquid medicine to be tested with a phosphate buffer solution, and testing to obtain absorbance A; and replacing the liquid medicine to be detected and the tyrosinase solution with a phosphate buffer solution, and testing to obtain an absorbance B.

Tyrosinase activation rate (%) [ (C-D) - (A-B) ]/(A-B) × 100%

Wherein, the higher the tyrosinase activation rate is, the stronger the melanin generation promoting ability is, namely, the better the hair blackening effect of the natural product monomer composition is.

Table 1: tyrosinase activation experiment result of natural product monomer composition

As can be seen from the experimental data in Table 1, the natural product monomer compositions of examples 1-3 all had significant tyrosinase activation. Among them, the tyrosinase activation rate was highest for the natural product monomer composition of example 1. Also, the tyrosinase activation rates of the natural product monomer compositions of examples 1-3 were significantly higher than the comparative examples 1-2, which used only a single natural product monomer.

As shown in the experimental results of the comparative example 1 group, the tyrosinase activation rate of pinocembrin alone was significantly lower than that of the example 1 group in which pinocembrin was used in combination with quercetin. As shown in the experimental results of the comparative example 2 group, the tyrosinase activation rate of quercetin alone is only 21.09%, which is almost equivalent to that quercetin does not have tyrosinase activation effect, and indeed some documents in the prior art report that quercetin may have tyrosinase inhibition effect. Surprisingly, however, the tyrosinase activation rate of the composition was significantly increased, up to 195.08%, when pinocembrin was combined with quercetin.

As shown in the experimental results of the comparative example 3 group, when the pinosylvin is used in combination with naringenin, naringenin cannot enhance the tyrosinase activation rate of pinosylvin although naringenin and quercetin belong to the same flavonoid natural products and have similar chemical structures.

Effect example 2: white to black test

100 white hair subjects (the number of white hairs is more than 100) were recruited and randomly divided into 5 groups of 20 persons each, half of both men and women. Group 1 was a blank control group using only a general pomade blank, and groups 2 to 5 used pomade bases including the natural product monomer compositions of example 1 and comparative examples 1 to 3, respectively. Besides, any product with the function of turning white hair into black hair is not used.

The test subject can uniformly apply the liquid medicine to the scalp, the hair root and the hair tip once a day in the morning and at night, and lightly massage the liquid medicine to ensure that the liquid medicine is uniformly absorbed. The number of white hairs in the head fixation area of the subject was recorded once a week, and each group of the test substance was continuously used for 4 weeks. The results of the experiment are shown in table 2 below:

table 2: effect of the Natural product monomer compositions of the invention on the number of white hairs in a subject

As can be seen from the experimental data in table 2, the example 1 group of natural product monomers was able to significantly reduce the number of white hairs in the subject. Among them, the reduction rate of white hair (42.62%) in the subject was significantly higher for the natural product monomer composition of example 1 group than for the comparative example 1-2 groups using only a single natural product monomer.

As shown in the experimental results of the comparative example 1 group, when pinocembrolide alone was used, the white hair reduction rate of the subjects was significantly lower than that of the example 1 group in which pinocembrolide was used in combination with quercetin.

As shown in the experimental results of the comparative example 2 group, unlike the results obtained in effect example 1, the use of quercetin alone can also slightly reduce white hair of the subject, which is presumed to be possibly related to the coloring effect of quercetin itself on hair.

As shown in the experimental results of the comparative example 3 group, when the pinosylvin is used in combination with naringenin, naringenin cannot enhance the rate of reduction of white hair of the subjects by the pinosylvin, although naringenin and quercetin belong to the same flavonoid natural products and have similar chemical structures.

Effect example 3: effect of the Natural product monomer composition of the invention on Hair growth in mice

Male C57BL/6J mice at 6-8 weeks of age were randomly divided into 5 groups of 10 mice each. Group 1 was a blank control group using only a general pomade blank, and groups 2 to 5 used pomade bases including the natural product monomer compositions of example 1 and comparative examples 1 to 3, respectively.

The test subjects were administered to each group 24h after shaving (3X 3cm) the backs of the mice, 1 time/day, for 21 consecutive days, after the experiment was completed, the experimental animals were euthanized, the newly grown hair lengths were measured and the test areas were counted for hair follicles.

Statistical data are expressed as mean ± standard error. Statistical analysis comparisons were made using analysis of variance with repeated measures, and differences between groups were compared using the Fisher LSD test.

The experimental data are shown in table 3 below.

Table 3: effect of the Natural product monomer compositions of the invention on mouse Hair growth Length and Hair follicle number (mean. + -. SE)

Note: ^** ，p<0.01, compared to the blank group; ^## ，p<0.01, compared with the group of comparative example 1

As can be seen from the experimental data in table 3, the natural product monomer of example 1 group significantly increased the hair growth length and number of hair follicles (p <0.01) in mice compared to the blank control group.

As shown in the experimental results of the comparative example 1 group, when pinocembrin alone was used, the hair growth length and the number of follicles of the mice were significantly inferior to those of the example 1 group in which pinocembrin was used in combination with quercetin, and the experimental results of the example 1 group were significantly different (p <0.01) from those of the comparative example 1 group.

As shown in the experimental results of the comparative example 2 group, quercetin alone only slightly increased the hair growth length and the number of follicles in the mice.

As shown in the experimental results of the comparative example 3 group, when pinosylvin is used in combination with naringenin, naringenin cannot enhance the effect of pinosylvin on the hair growth and the number of hair follicles in mice, although naringenin and quercetin belong to the same flavonoid natural products and have similar chemical structures.

It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims

1. A natural product monomer composition with the functions of blacking and strengthening the hair is characterized in that: the natural product monomer composition comprises pinocembrin and quercetin, wherein the proportion of the pinocembrin and the quercetin in the composition in parts by weight is 10: 1-1: 10.

2. the composition of claim 1, wherein: wherein the proportion of the pinosylvin and the quercetin in the composition in parts by weight is 5: 1-1: 5.

3. the composition according to claim 1 or claim 2, characterized in that: wherein the proportion of the pinosylvin and the quercetin in the composition in parts by weight is 5: 1. 4: 1. 3: 1. 2: 1. 1: 1. 1: 2. 1: 3. 1: 4 or 1: 5.

4. the composition according to any one of claims 1 to 3, characterized in that: the composition significantly enhances tyrosinase activity, and promotes telogen-phase hair follicles to enter the regenerative cycle, promoting hair growth.

5. Use of the composition of any one of claims 1 to 4 in the preparation of a hair-blackening and hair-care product.

6. Use according to claim 5, characterized in that: the product is an external product.

7. Use according to claim 5, characterized in that: the product is a medicament or a cosmetic.

8. Use according to claim 6, characterized in that: the external product is gel, ointment, cream, foam, shampoo, hair conditioner or spray type liquid preparation.